ISSN:
1432-203X
Keywords:
KeywordsDoritaenopsis
;
Cryopreservation
;
Abscisic acid
;
Protocorm-like body
;
Abbreviations ABA: Abscisic acid
;
BA6: Benzylaminopurine
;
DMSO: Dimethylsulphoxide
;
NAA: Naphthaleneacetic acid
;
ND: New¶Dogashima
;
PLB: Protocorm-like body
;
TTC: 2,3,5-Triphenyltetrazolium chloride
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1–3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazolium chloride stainability) was obtained when the cells were precultured in liquid New Dogashima medium with 0.1 M sucrose and 1.0 mg/l abscisic acid for 1 week at 25 °C in the light. Dehydration by PVS2 was important for the cryopreservation of Doritaenopsis cells. Protocorm-like bodies were induced from cryopreserved cells without morphological variations.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990000255
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