Abstract
Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1–3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazolium chloride stainability) was obtained when the cells were precultured in liquid New Dogashima medium with 0.1 M sucrose and 1.0 mg/l abscisic acid for 1 week at 25 °C in the light. Dehydration by PVS2 was important for the cryopreservation of Doritaenopsis cells. Protocorm-like bodies were induced from cryopreserved cells without morphological variations.
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Received: 18 January 2000 / Revision received: 16 June 2000 / Accepted: 22 June 2000
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Tsukazaki, H., Mii, M., Tokuhara, K. et al. Cryopreservation of Doritaenopsis suspension culture by vitrification. Plant Cell Reports 19, 1160–1164 (2000). https://doi.org/10.1007/s002990000255
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DOI: https://doi.org/10.1007/s002990000255