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  • 1
    Electronic Resource
    Electronic Resource
    Cryopreservation of Doritaenopsis suspension culture by vitrification (2000)
    Springer
    Plant cell reports 19 (2000), S. 1160-1164 
    Details
    ISSN: 1432-203X
    Keywords: KeywordsDoritaenopsis ; Cryopreservation ; Abscisic acid ; Protocorm-like body ; Abbreviations ABA: Abscisic acid ; BA6: Benzylaminopurine ; DMSO: Dimethylsulphoxide ; NAA: Naphthaleneacetic acid ; ND: New¶Dogashima ; PLB: Protocorm-like body ; TTC: 2,3,5-Triphenyltetrazolium chloride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1–3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazolium chloride stainability) was obtained when the cells were precultured in liquid New Dogashima medium with 0.1 M sucrose and 1.0 mg/l abscisic acid for 1 week at 25  °C in the light. Dehydration by PVS2 was important for the cryopreservation of Doritaenopsis cells. Protocorm-like bodies were induced from cryopreserved cells without morphological variations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000255
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  • 2
    Electronic Resource
    Electronic Resource
    Helicoidal distributed-feedback cavity action in a ferroelectric liquid crystal (1995)
    Springer
    Applied physics 60 (1995), S. 485-487 
    Details
    ISSN: 1432-0649
    Keywords: 42.80. Jr ; 78.20. Wc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Second-Harmonic (SH) generation was observed in homeotropically aligned ferroelectric liquid-crystal cells using fundamental light propagating along the helicoidal axis. Relatively strong SH light was observed in the helicoidal structure when the optical pitch was nearly the same as the SH-light wavelength, though, otherwise, the cancellation of the generated SH light by the helicoid results in negligibly weak SH intensity. Because the observed SH light is independent of the cell thickness, the SH light is attributed to the one generated from the surface region and the SH light generated inside the cell is confined and lost by the helicoid. This phenomenon indicates the helicoidal Distributed-FeedBack (DFB) cavity action and suggests the possibility of the helicoidal DFB laser using dye-doped ferroelectric liquid-crystal cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01081331
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