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  • 1
    Electronic Resource
    Electronic Resource
    Cryopreservation of Doritaenopsis suspension culture by vitrification (2000)
    Springer
    Plant cell reports 19 (2000), S. 1160-1164 
    Details
    ISSN: 1432-203X
    Keywords: KeywordsDoritaenopsis ; Cryopreservation ; Abscisic acid ; Protocorm-like body ; Abbreviations ABA: Abscisic acid ; BA6: Benzylaminopurine ; DMSO: Dimethylsulphoxide ; NAA: Naphthaleneacetic acid ; ND: New¶Dogashima ; PLB: Protocorm-like body ; TTC: 2,3,5-Triphenyltetrazolium chloride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1–3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazolium chloride stainability) was obtained when the cells were precultured in liquid New Dogashima medium with 0.1 M sucrose and 1.0 mg/l abscisic acid for 1 week at 25  °C in the light. Dehydration by PVS2 was important for the cryopreservation of Doritaenopsis cells. Protocorm-like bodies were induced from cryopreserved cells without morphological variations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000255
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  • 2
    Electronic Resource
    Electronic Resource
    Computer-aided peptide sequencing of an unknown cyclic peptide from Bacillus subtilis (1988)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 23 (1988), S. 290-291 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210230415
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  • 3
    Electronic Resource
    Electronic Resource
    Computer-aided peptide sequencing by fast atom bombardment mass spectrometry (1986)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 13 (1986), S. 373-380 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This paper proposes an efficient method for computer-aided peptide sequencing based on fast atom bombardment mass spectrometry. The method allows us to analyse peptides much larger than 1000 u within a reasonable cpu time and to have a set of amino acid sequences which are consistent with the observed fast atom bombardment mass spectrum to varying degrees. Another feature is that there is no need to pick sequence ion peaks out of the spectrum; all the ions observed are equally taken into account in the analysis so that one can exhaust the possibilities expected from the whole spectrum. For most samples of penta- to tridecapeptides, parts of an amino acid sequence could be reproduced satisfactorily without prior knowledge of amino acid composition. The degree of success in sequence elucidation depends entirely on the spectrum used in the analysis. When fragment ion peaks are clearly observed, the sequence can be predicted successfully. The result obtained for a given amino acid composition was always satisfactory.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200130709
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  • 4
    Electronic Resource
    Electronic Resource
    Sequence determination of unknown cyclic peptide antibiotics by fast atom bombardment mass spectrometry (1990)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 19 (1990), S. 395-399 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The primary structures of unknown cyclic peptides produced by a Bacillus strain have been determined by fast atom bombardment (FAB) mass spectrometry, which has established the peptides as a new family of the iturin group antibiotics. FAB mass spectra of the intact peptides gave the immonium ions characteristic of constituent amino acids which made it possible to distinguish Asn from Asp and Glu from Gln. Collision-induced dissociation (CID) spectra of protonated molecules provided complete information on the connectivity of amino acid residues but did not reveal the direction of peptide bonding, while those obtained for fragment ions allowed us to make a discrimination between the correct sequence and its retro sequence. The amino acid sequences derived are c(Thr-Xaa-Asn-Tyr-Asn-Ser-Glu-Ser) (Xaa: C14 or C15 β-amino acid) which are closely related to that of bacillomycin L. The structure is confirmed by the FAB mass spectra of the partial acid hydrolysate and the peptide mixture obtained from its single-step Edman degradation. Fragmentation processes involved in the CID spectra of the cyclic peptides are discussed based on the established amino acid sequence.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200190703
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  • 5
    Electronic Resource
    Electronic Resource
    Raman scattering from phonons in MnCO3 (1983)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 14 (1983), S. 330-332 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Measurements of Raman scattering from the vibronic origin in MnCO3 (rhodochrosite) are reported. The observed five Raman lines (184, 289, 719, 1086 and 1415 cm-1) are identified with and classified into the relevant phonon modes. Those lines show the theoretically expected polarization characteristics.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250140507
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