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  • 11
    ISSN: 1432-0789
    Keywords: Key words Nitrous oxide ; Methane ; Clover ; Herb ley ; Cultivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Nitrous oxide (N2O) emissions and methane (CH4) consumption were quantified following cultivation of two contrasting 4-year-old pastures. A clover sward was ploughed (to 150–200 mm depth) while a mixed herb ley sward was either ploughed (to 150–200 mm depth) or rotovated (to 50 mm depth). Cumulative N2O emissions were significantly greater following ploughing of the clover sward, with 4.01 kg N2O-N ha–1 being emitted in a 48-day period. Emissions following ploughing and rotovating of the ley sward were much less and were not statistically different from each other, with 0.26 and 0.17 kg N2O-N ha–1 being measured, respectively, over a 55-day period. The large difference in cumulative N2O between the clover and ley sites is presumably due to the initially higher soil NO3 – content, greater water filled pore space and lower soil pH at the clover site. Results from a denitrification enzyme assay conducted on soils from both sites showed a strong negative relationship (r=–0.82) between soil pH and the N2O:(N2O+N2) ratio. It is suggested that further research is required to determine if control of soil pH may provide a relatively cheap mitigation option for N2O emissions from these soils. There were no significant differences in CH4 oxidation rates due to sward type or form of cultivation.
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 28 (1999), S. 253-258 
    ISSN: 1432-0789
    Keywords: Key words Grazing animals ; Enzyme activity ; Microbial biomass ; Pasture ; Soil organic matter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The size and activity of the soil microbial biomass in grazed pastures was compared on the main grazing area and on stock camp areas where animals congregate. Two sites were on hill country and three on gently sloping border-dyke irrigated land. Due to the transfer of nutrients and organic matter to the camp areas via dung and urine there was an accumulation of soil organic C, organic and inorganic P and S and soluble salts in the camp areas. Soil pH also tended to be higher in camp areas due to transfer of alkalinity by the grazing animals. Water soluble organic C, microbial biomass C and basal respiration were all higher in soils from camp areas but the proportion of organic C present as microbial C and the microbial respiratory quotient were unaffected. Microbial activity as quantified by arginine ammonification rate and fluorescein diacetate (FDA) hydrolysis was higher in camp than non-camp soils but dehydrogenase activity remained unaffected. Activities of protease, histidase, urease, acid phosphatase and aryl-sulphatase were all higher in stock camp soils. The activities of both histidase and aryl-sulphatase were also higher when expressed per unit of microbial biomass C, indicating that the increased activity was the result of increased enzyme production by the microbial community. Prolonged regular applications of dairy shed effluent (diluted dung and urine from cattle) to a field had a similar effect to stock camping in increasing soil organic matter content, nutrient accumulation and soil biological activity. It was concluded that the stock camping activity of grazing animals results in an increase in both the fertility and biological activity in soils from camp areas at the expense of these properties on the main grazing areas.
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  • 13
    ISSN: 1432-0789
    Keywords: Key words Nitrous oxide ; Clover ; Herb ley ; Soil pH ; Allium cepa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  N2O emissions were measured from three contrasting onion (Allium cepa L.) production systems over an 8.5-month period. One system was established on soil where a clover sward had 3 months earlier been ploughed in (ploughed clover site). This production system followed conventional production management practices. The other two systems were established on soil where a mixed herb ley had 3 months earlier been either ploughed or rotovated. These last two production systems followed the guidelines of the International Federation of Organic Agriculture Movements (IFOAM). Cumulative N2O emissions were significantly greater from the ploughed clover site compared to the ploughed ley site (3.8 and 1.6 kg N2O-N ha–1, respectively), while cumulative N2O emissions from the ploughed ley and rotovated ley sites were not significantly different from each other. Emissions from all sites were dominated by episodes of high N2O flux activity following seedbed preparation and drilling, when soil water suction (SWS) was shown to be the rate-controlling variable. The decline in the N2O fluxes after these peak emissions followed clear exponential relationships of the form F=Ae– kt (r≥0.91), where F is the daily flux and A is the y-intercept. First-order decay constants (k) during these periods of declining N2O fluxes (corresponding to half-lives of 2.6–3.0 days) were not significantly different in magnitude from the first-order rate constants that characterised the increasing SWS. Gross differences in cumulative emissions between the clover and ley sites were attributed to the influence of differing soil pHs at the two sites on the N2O:(N2O+N2) ratio in the denitrification products. It also appeared that fertiliser applications to the clover site had both direct and indirect effects on N2O emissions by: (1) enhancing N2O emissions via potential nitrification, (2) increasing the NO3 – supply for enhanced N2O emissions via denitrification, and (3) influencing the N2O:(N2O+N2) ratio by lowering soil pH and increasing NO3 – concentrations. Onion crop yields were greater at the clover site, mainly due to the higher density of planting made possible under a conventional production philosophy. Expressing the yield on the basis of net N2O emissions, 23 t onions kg–1 N2O-N was obtained from the ploughed clover, which was double that obtained for the two systems based on the ley site. However, when the N2O emissions from the cultivation of the soils prior to the sowing of the onions was included, all three systems produced a similar yield per kilogram of N2O-N emitted, averaging 10 t kg–1.
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  • 14
    ISSN: 1432-0789
    Keywords: Earthworms ; Enzyme activity ; Microbial biomass ; Pasture ; Soil organic matter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We investigated the quantity and distribution of organic C, microbial biomass C, protease, arylsulphatase and arylphosphatase activity, and earthworm numbers and biomass in the soil from a 37-year-old grazed pasture supplied with superphosphate at rates of 0, 188, and 376 kg ha-1 annually. The results were compared with a non-irrigated wilderness site which had not been used for agriculture and an arable site that had been intensively cultivated for 11 consecutive years. In the 0- to 5-cm layer, organic C followed the trend arable〈wilderness = control〈low phosphate = high posphate and soil biological activity generally followed a similar trend. For example, protease and arylsulphatase activity and microbial biomass C followed the order arable〈wilderness〈control〈low phosphate = high phosphate. The greater activity in the control than the wilderness site was attributed to the more regular turnover of organic matter throughout the year in the control due to the activity of the grazing animals. Earthworm numbers increased in the order arable〈wilderness〈control〈low phosphate〈high phosphate. In the improved pasture sites the earthworm population was dominated by Aporrectodea caliginosa (77–89% of total numbers) although Lumbricus rubellus made an increasing contribution to the population with increasing superphosphate rates. In the unirrigated wilderness site the population consisted of 56% A. caliginosa and 44% L. rubellus. While Octolasion cyaneum and A. rosea made up a small proportion of the population in the improved pasture sites, they were not present in the wilderness or arable sites. A. caliginosa was the only species present in the arable site. The mean fresh weight of individuals followed the order arable〈control = low phosphate = high phosphate〈wilderness and the proportion of jeveniles in the population was greatest in the arable and lowest in the wilderness site.
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 107 (1976), S. 63-70 
    ISSN: 1432-072X
    Keywords: Yeast mitochondria ; Cytochrome c peroxidase ; Catalase ; Peroxisomes ; Petite mutation ; Cytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytochemical and ultrastructural analysis of wild-type cells of Saccharomyces cerevisiac, grown aerobically in a glucose-limited chemostat, shows that cytochrome c peroxidase is localized between the membranes of the cristae, that is, in the intracristal space. This enzyme is thus positioned appropriately within the organelle to act as an alternate terminal oxidase for the respiratory chain. The proximity of the peroxidase to major sites of generation of its two substrates may account for the small leakage of hydrogen peroxide from yeast mitochondria, as compared with the larger outflow from mammalian mitochondria. In the cytoplasmic petite mutant, gross distortion of promitochondrial membrane arrangement is evident. Nevertheless, cytochrome c peroxidase activity is present in the same amounts as is found in wildtype cell, and is localized predominantly within annuli of membrane which constitute the promitochondria in these cells. No unequivocal evidence was obtained for the localization of catalase in microbodies or other organelles in either wild-type or petite cells.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 110 (1976), S. 173-175 
    ISSN: 1432-072X
    Keywords: Rust ; Barley ; Axenic culture ; Puccinia striiformis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Uredospores of a barley isolate ofPuccinia striiformis West. were germinated on glucose peptone agar and the development of colonies was observed with a microscope. A small number (0.1–1%) of germ tubes did not abort during the first 20 h of incubation and either differentiated an infection structure or continued slowly to elongate for several days. In 4 out of 9 experiments some of these sporelings produced colonies that reached maximum size (0.15–0.20 mm) after 3 weeks at 15° C.
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Annales geophysicae 14 (1997), S. 1480-1486 
    ISSN: 0992-7689
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The random-noise errors involved in measuring the Doppler shift of an ‘incoherent-scatter’ spectrum are predicted theoretically for all values of Te/Ti from 1.0 to 3.0. After correction has been made for the effects of convolution during transmission and reception and the additional errors introduced by subtracting the average of the background gates, the rms errors can be expressed by a simple semi-empirical formula. The observed errors are determined from a comparison of simultaneous EISCAT measurements using an identical pulse code on several adjacent frequencies. The plot of observed versus predicted error has a slope of 0.991 and a correlation coefficient of 99.3%. The prediction also agrees well with the mean of the error distribution reported by the standard EISCAT analysis programme.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Annales geophysicae 14 (1997), S. 1403-1412 
    ISSN: 0992-7689
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Incoherent-scatter radar and ionospheric sounding are powerful and complementary techniques in the study of the Earth’s ionosphere. The work presented here involves the use of the Tromsø Dynasonde as a correlative diagnostic with the EISCAT incoherent-scatter radar. A comparison of electron-density profiles shows how a Dynasonde can be used to calibrate an incoherent-scatter radar and to monitor changes in the system. Sky-maps of the direction of Dynasonde echoes are compared with EISCAT-derived density profiles to illustrate how a Dynasonde can be used to measure the drift velocity of auroral features. Vector velocities fitted to Dynasonde echoes are compared with EISCAT-derived plasma velocities. The results show good agreement when the data are taken during quiet to moderately active conditions and averaged over time scales of 30 min or more.
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  • 19
    ISSN: 0992-7689
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract EISCAT observations of interplanetary scintillation have been used to measure the velocity of the solar wind at distances between 15 and 130R⊙ (solar radii) from the Sun. The results show that the solar wind consists of two distinct components, a fast stream with a velocity of ∼ 800 km s−1 and a slow stream at ∼ 400 kms−1. The fast stream appears to reach its final velocity much closer to the Sun than expected. The results presented here suggest that this is also true for the slow solar wind. Away from interaction regions the flow vector of the solar wind is purely radial to the Sun. Observations have been made of fast wind/slow wind interactions which show enhanced levels of scintillation in compression regions.
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  • 20
    ISSN: 0992-7689
    Keywords: Solar physics ; Astrophysics ; Astronomy ; Magnetic fields ; Space plasma physics ; Charged particle motion and acceleration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract EISCAT observations of the interplanetary scintillation of a single source were made over an extended period of time, during which the orientation of the baselines between the two observing sites changed significantly. Assuming that maximum correlation between the scintillations observed at the two sites occurs when the projected baseline is parallel to the direction of plasma flow, this technique can be used to make a unique determination of the direction of the solar wind. In the past it has usually been assumed that the plasma flow is radial, but measurements of eleven sources using this technique have indicated conclusively that in at least six cases observed at mid or high heliocentric latitude there is a significant non-radial component directed in four cases towards the heliocentric equator and in two cases towards the pole.
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