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  • 1
    Electronic Resource
    Electronic Resource
    Revertants of the amylose-free potato clone 86.040 (2n = 1x = 12)
    Amsterdam : Elsevier
    Plant Science 67 (1990), S. 177-182 
    Details
    ISSN: 0168-9452
    Keywords: granule bound starch synthase ; mutation ; potato ; reversion ; starch composition
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(90)90241-F
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  • 2
    Electronic Resource
    Electronic Resource
    Development of a cyclic somatic embryogenesis regeneration system for leek (Allium ampeloprasum L.) using zygotic embryos (1995)
    Springer
    Plant cell reports 14 (1995), S. 227-231 
    Details
    ISSN: 1432-203X
    Keywords: leek ; Allium ampeloprasum L. ; somatic embryogenesis ; genotypic effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In leek (Allium ampeloprasum L.) a cyclic system of somatic embryogenesis was developed. Somatic embryos used for cyclic embryogenesis were able to develop the same type of embryogenic callus as zygotic embryos in the primary cycle. For the first time a comparison of the efficiencies of both expiants was made. Ten families were investigated for somatic embryogenesis. There was a genetic relationship with respect to somatic embryo production between the reciprocal crosses. From each family one genotype was selected for investigating cyclic somatic embryogenesis. Different levels of somatic embryo production were found between the expiants of zygotic and somatic embryos. The two best genotypes, 92.001-03 and 92.002-33 produced twice as many somatic embryos as the overall average. On average, 56% of the somatic embryos finally developed into greenhouse plantlets.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233638
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  • 3
    Electronic Resource
    Electronic Resource
    Increased frost tolerance and amino acid content in leaves, tubers and leaf callus of regenerated hydroxyproline resistant potato clones (1987)
    Springer
    Euphytica 36 (1987), S. 369-380 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; amino acids ; amino acid analogue resistance ; frost tolerance ; plant regeneration ; proline ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A number of previously selected hydroxyproline (hyp) resistant cell lines of a diploid potato (Solanum tuberosum L., clone H2578, 2n=2x=24) could be regenerated into plants which were further analysed. Hyp resistance, although lower than in the originally selected calli, was still present in regenerated shoots and in callus initiated from these shoots and it was not lost upon (mini)tuber propagation. Regenerated shoots showed a wide range of phenotypic variation. The chromosome number, analysed in 4 clones, appeared to be hypotetraploid (44 or 45). Tuber-propagated regenerants generally showed increased frost tolerance both at the plant and the cell level. In leaves this seemed to be associated with increased levels of both proline and total amino acid content. However, in callus only the total amino acid content but not proline was still elevated. In the wild type the frost tolerance of the tubers appeared to be lower than that of the leaves. Between tubers of wild type and the hyp resistant regenerants no differences in frost tolerance were found, although proline and total amino acid content tended to be higher in tubers from the regenerants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041480
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  • 4
    Electronic Resource
    Electronic Resource
    Phenotypic and genotypic characterization of an amylose-free starch mutant of the potato (1989)
    Springer
    Euphytica 44 (1989), S. 43-48 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; mutant ; starch composition ; amylose-free ; embryo rescue ; inheritance ; phenotype ; breeding ; karyotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The amylose-free (amf) potato mutant 86.040 has been characterized phenotypically and genotypically. Not only storage starch in tubers and metabolic starch in leaves but also starch in cells with specific functions, such as columella cells in the root cap and guard cells of stomata, was amylose-free. Doubled amf-clones of 86.040 flowered well, but the percentage of stainable pollen was low (8.2%). Therefore, they were used as female parent in crosses with diploid amylose-containing plants. Over 400 pollinations gave 13 berries, all dropping off prematurely; they contained 78 ovules with embryos at different developmental stages. Twenty F1 plants were obtained by embryo rescue, all having amylose starch. Three of them were characterized more extensively. They flowered well and pollen stainabilitity as well as seed set were high. Analysis of the F1 plants and of F1 × F1 progeny plants showed that the amf-character is monogenic and recessive. The locus involved was designated amf +and the mutated allele amf.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022597
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  • 5
    Electronic Resource
    Electronic Resource
    Introduction of an amylose-free (amf) mutant into breeding of cultivated potato, Solanum tuberosum L. (1991)
    Springer
    Euphytica 53 (1991), S. 247-253 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; amylose-free mutant ; unreduced gametes ; analytic breeding ; desynapsis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Using an amylose-free (amf)mutant of diploid potato (Solanum tuberosum), diploid and tetraploid clones with different genotypes at the amf-locus were produced. In order to make use of the diploid material in analytic breeding of amf-potatoes, clones were selected that produced a considerable frequencies of 2n-pollen and 2n-eggs. Successful attempts were made to select normal synaptic as well as desynaptic clones producing 2n-gametes. Based on the phenotype of starch in the microspores, tetraploid clones with nulliplex, simplex and duplex genotypes at the amf-locus were selected. Prospects of using this material in conventional as well as in analytic breeding of potato are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023276
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  • 6
    Electronic Resource
    Electronic Resource
    Spontaneous and induced loss of chromosomes in slow-growing somatic hybrid calli of Solanum tuberosum and Nicotiana plumbaginifolia (1991)
    Springer
    Euphytica 56 (1991), S. 287-296 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; Nicotiana plumbaginifolia ; asymmetric hybrids ; radiation damage ; electrofusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rate and extent of spontaneous and induced chromosome loss have been determined at the callus level of somatic hybrids of mutants of Solanum tuberosum and Nicotiana plumbaginifolia. AEC (amino ethyl cystein) resistance in potato and Nitrate-Reductase deficiency in N. plumbaginifolia have been used as genetic markers and chromosome morphology as a cytological marker. In this combination, development of hybrid callus was late and slow. Only a limited number of non-regenerable hybrid calli have become available. Chromosome loss could clearly be established in these hybrids from loss of markers and from chromosome cytology. Loss of markers occurred independantly. The best conditions to induce loss of chromosomal material in donor cells by irradiation were found by cytological investigations. A very drastic reduction in chromosome transfer by fusion could be effected by irradiation of plant tissue and subsequent preparation of protoplasts after a few days. Following fusion, hybrid callus was recovered with the potato genome drastically reduced. The amount of loss was deduced from the presence of a few fragments in metaphase cells or from interphase nuclei after in situ hybridization with a repetitive potato DNA probe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042374
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  • 7
    Electronic Resource
    Electronic Resource
    Plant regeneration via organogenesis and somatic embryogenesis in callus cultures derived from mature zygotic embryos of leek (Allium ampeloprasum L.) (1996)
    Springer
    Euphytica 91 (1996), S. 261-270 
    Details
    ISSN: 1573-5060
    Keywords: Allium ampeloprasum ; 2,4-dichlorophenoxy acetic acid ; Murashige and Skoog medium ; organogenesis ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A high frequency plant regeneration system via organogenesis and somatic embryogenesis was established with callus cultures derived from mature zygotic embryos of different leek genotypes (Allium ampeloprasum L.). Four different callus types with varying morphogenetic potential were obtained. Relatively high concentrations of the auxin 2,4-dichlorophenoxy-acetic acid reduced callus weight and subsequent shoot regeneration and primordia formation of the callus. Shoot regeneration and primordia formation of the callus decreased after prolonged subculture on media containing 2,4-dichlorophenoxy acetic acid. A callus growth period of six weeks on Murashige and Skoog medium with 0.25–0.5 mg l-1 2,4-dichlorophenoxy acetic acid showed the highest rate of shoot regeneration after transfer of callus to regeneration medium with 1 mg l-1 kinetin. Differences between leek genotypes in callus type, callus weight, shoot regeneration and primordia formation were observed. Histological observations showed that plant regeneration took place, both via the pathway of somatic embryogenesis and organogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033086
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  • 8
    Electronic Resource
    Electronic Resource
    Behaviour of genetically modified amylose free potato clones as progenitors in a breeding program (1997)
    Springer
    Euphytica 98 (1997), S. 169-175 
    Details
    ISSN: 1573-5060
    Keywords: potato ; Solanum tuberosum ; genetic modification ; amylose free ; transgenic clones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three amylose-free genetically modified potato clones were used both as male and female parents in a breeding program with non-GMO potato clones. Segregation data on the expression of the inserted antisense gene construct in tubers of progeny plants were in agreement with previous molecular analysis of the transgenic clones. The inheritance of the inserted genes was according to Mendelian segregation. Therefore, these clones can be very useful in a breeding program for large scale introduction of amylose free potato cultivars into agriculture. Because of varying number and expression levels of inserts in the GMO-clones, but also because of the varying strength of the endogenous GBSS-alleles of the non-GMO-clones, a segregation into a range of amylose contents occurred. The segregation of the starch colour after iodine staining of pollen of transgenic clones did not follow the obtained segregation in the progeny and was, therefore, not useful in predicting the breeding result.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003109015151
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  • 9
    Electronic Resource
    Electronic Resource
    Cytological studies on adventitious shoots and minitubers of a monoploid potato clone (1988)
    Springer
    Euphytica 39 (1988), S. 213-219 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; adventitious shoot induction ; minituber induction ; starch composition ; cytology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A three step procedure for adventitious shoot regeneration on leaf explants of monoploid potato clone H7322 and a minituber induction procedure on stem segments have been described. Chromosome counts on 92 adventitious shoots showed that 85% of them had been polyploidized, i.e., 71% were diploid, 1% tetraploid, and 13% were mixoploid. Cytophotometric studies on nuclei of soil grown tubers of tetraploid cv Astarte, of 1x, 2x and 4x adventitious shoots of H7322, and of diploid H2578 showed in all cases polyploidization with prominent classes up to 8C and 16C. However, nuclei of pith cells of 5 weeks old minitubers which had developed on monoploid H7322 itself or on 1x adventitious shoots of H7322 showed predominantly 1C and 2C values. Pith cells of minitubers of monoploid H7322 were screened, after iodine staining, for the presence of variant cells containing reddish-brown staining (amylose-free) starch. In more than 75% of the investigated minitubers one or a few of such variant cells were found indicating that such a variation occurs in minitubers of monoploid potato and that this variant character is expressed in cells of vegetative storage organs like potato tubers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037098
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  • 10
    Electronic Resource
    Electronic Resource
    Isolation and characterisation of somatic hybrids of diploid Solanum tuberosum and Solanum brevidens and the use of amylose-free starch mutation for detection of introgression (1993)
    Springer
    Euphytica 69 (1993), S. 191-201 
    Details
    ISSN: 1573-5060
    Keywords: interspecific somatic hybridisation ; male and female fertility ; meiotic recombination ; potato ; Solanum brevidens ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Somatic hybrids of diploid amylose-free (amf) Solanum tuberosum and diploid S. brevidens were made by Poly-Ethylene-Glycol (PEG) or electrofusion methods. For the isolation of interspecific hybrids the use of selection markers (kanamycin and hygromycin resistance) was useful but not essential. In this 2x+2x interspecific combination 4x and 6x somatic hybrids were obtained. Seed set was the best in 4x×4x (S. tuberosum) backcrosses, but seed germination was the best in 6x×4x combinations, using in vitro germination of unripe seeds harvested 25 days after pollination. A high degree of pollen stainability (30–40%) was observed in 7 tetraploid hybrids and very low in all hexaploids. After iodine staining, the recessive amf marker was expressed by a red colour instead of blue, visible in starch-containing cells like columella cells of root tips, (micro)tubers or microspores. As expected, complementation was observed in starch-containing cells of the fusion hybrids. Segregation of the amf marker was clearly observed in microspores of 4x and 6x hybrids. Segregation ratios in the 4x hybrids showed variable recombination frequencies. In the backcross progeny of hexaploid F12-5 with a tetraploid amf mutant one amylose-free recombinant among 67 plants was found, indicating the occurrence of meiotic recombination in the megaspore mother cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022365
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