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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 58 (1980), S. 241-246 
    ISSN: 1432-2242
    Keywords: Brassica ; Pollen germination ; Pollen surface ; Self-incompatibility ; Stigma surface
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Recent studies on the mechanism of self-incompatibility in Brassica indicate the location, nature and mode of action of the molecules involved. Characteristics of the pollen surface and the stigma surface are described in detail, together with new information pertaining to the recognition molecules located therein. A sequence of events is outlined leading from pollination, through adhesion, hydration, germination, and tube growth to acceptance and ultimate compatibility. The characteristics of rejection of incompatible grains are described for each stage of the pollen-stigma interaction. It is proposed that recognition of proteins from the coating of self-pollen by the molecules in the pellicle results in the formation of a biologically-active complex which inhibits water supply to the incompatible grain, and that all other manifestations of incompatibility are a consequence of this initial response.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Planta 146 (1979), S. 211-216 
    ISSN: 1432-2048
    Keywords: Brassica ; Pollen adhesion ; Pollen hydration ; Self-incompatibility ; Tryphine mobility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Quantitative studies of the adhesion of pollen grains to the stigma in Brassica oleracea revealed that self-pollen is initially less firmly bound than cross-pollen. The pollen grain tryphine, believed to be important in the adhesion process, has been shown to differ in mobility following self- and cross-pollination when observed using fluorescent probes. The hydration of the pollen grains has been investigated in vitro by measuring the changes in shape, volume and fresh weight of the imbibing grains. Whilst little change in volume could be detected there was a considerable increase in fresh weight together with a change of shape. The significance of these events, which occur prior to pollen germination, is discussed in relation to their effect upon subsequent germination and expression of self-incompatibility.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2048
    Keywords: Brassica ; Cellular recognition ; Glycoproteins ; Self-incompatibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Iso-electric focusing of extracts derived from stigmatic homogenates of Brassica oleracea reveals that the mature stigma possesses large quantities of a glycoprotein not present in earlier stages of development in the bud. Pollen germination experiments carried out in parallel with the biochemical tests suggest that the appearance of this glycoprotein, which has an isoelectric point of pH 5.8, is coincident with the development of the self-incompatibility response. The site of this protein, and the role it may play in pollen-stigma interactions are discussed in terms of current models of the self-incompatibility system in Brassica.
    Type of Medium: Electronic Resource
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