Abstract
Quantitative studies of the adhesion of pollen grains to the stigma in Brassica oleracea revealed that self-pollen is initially less firmly bound than cross-pollen. The pollen grain tryphine, believed to be important in the adhesion process, has been shown to differ in mobility following self- and cross-pollination when observed using fluorescent probes. The hydration of the pollen grains has been investigated in vitro by measuring the changes in shape, volume and fresh weight of the imbibing grains. Whilst little change in volume could be detected there was a considerable increase in fresh weight together with a change of shape. The significance of these events, which occur prior to pollen germination, is discussed in relation to their effect upon subsequent germination and expression of self-incompatibility.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- RH:
-
relative humidity
- SI:
-
self-incompatibility
- ConA:
-
concanavalin A
- I-ANS:
-
I-anilino-napthyl-sulfonic acid
References
Carter, A. L., McNeilly, T.: Effects of increased humidity on pollen growth and seed set following self-pollination in Brussels sprout (Brassica oleracea var. gemmifera). Euphytica 24, 805–813 (1975)
Carter, A. L., McNeilly, T.: Increased atmospheric humidity post pollination: a possible aid to the production of inbred line seed from mature flowers in the Brussels sprout (Brassica oleracea var. gemmifera). Euphytica 25, 531–536 (1976)
Carter, A. L., Williams, S. T., McNeilly, T.: Scanning electron microscope studies of pollen behaviour on immature and mature Brussels sprout (Brassica oleracea var. gemmifera) stigmas. Euphytica 24, 133–141 (1975)
Dickinson, H. G., Lewis, D.: Cytochemical and ultrastructural differences between intra-specific compatible and incompatible pollinations in Raphanus. Proc. R. Soc. London. Ser. B 183, 21–38 (1973)
Ferrari, T. E., Wallace, D. H.: Germination of Brassica pollen and expression of incompatibility in vitro. Euphytica 24, 757–765 (1975)
Ferrari, T. E., Wallace, D. H.: Pollen protein synthesis and control of incompatibility in Brassica. Theor. Appl. Genet. 48, 1243–1249 (1976)
Ferrari, T. E., Wallace, D. H.: A model for self-recognition and regulation of the incompatibility response of pollen. Theor. Appl. Genet. 50, 211–225 (1977)
Gilissen, L. J. W.: The influence of relative humidity on the swelling of pollen grains in vitro. Planta 137, 299–301 (1977)
Hale, L. J.: Biological Laboratory Data. London: Methuen 1965
Heslop-Harrison, Y.: Localization of concanavalin A binding sites on the stigma surface of a grass species. Micron 7, 33–36 (1976)
Heslop-Harrison, Y., Shivanna, K. E.: The receptive surface of the angiosperm stigma. Ann. Bot. 41, 1233–1258 (1977)
Kwack, B.: Stylar culture of pollen and physiological studies of self-incompatibility in Oenothera organensis. Physiol. Plant. 18, 297–305 (1965)
Ockendon, D. J.: Effect of hexane and humidity on self-incompatibility in Brassica oleracea. Theor. Appl. Genet. 52, 113–117 (1978)
Roggen, H.: Scanning electron microscopical observations on compatible and incompatible pollen stigma interactions in Brassica. Euphytica 21, 1–10 (1972)
Roggen, H.: Stigma application of an extract from rape pollen (Brassica rapus L.) effects self-incompatibility in Brussels sprout (Brassica oleracea L. var. gemmifera DC). Incompatibility Newslett. 6, 80–84 (1975)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stead, A.D., Roberts, I.N. & Dickinson, H.G. Pollen-pistil interaction in Brassica oleracea . Planta 146, 211–216 (1979). https://doi.org/10.1007/BF00388234
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00388234