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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Planta 182 (1990), S. 129-135 
    ISSN: 1432-2048
    Keywords: Arum ; Brassica (β-oxidation) ; β-Oxidation ; (enzyme location) ; Microbody ; Mitochondrion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract β-Oxidation enzymes were detected both in the mitochondria and microbodies of Arum maculatum L. spadices and Brassica napus L. seeds. It is apparent that the mitochondrial membrane barrier, which remains intact after sucrose-density-gradient centrifugation, prevents rapid access of acyl-GoA substrates to matrix βoxidation tes. Thus intact mitochondria showed little β-oxidation enzyme activity. Rupturing of the mitochondrial membrane allowed rapid access of acyl CoAs to matrix sites. Consequently, in ruptured mitochondria, high β-oxidation enzyme activities were measured.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Planta 168 (1986), S. 261-266 
    ISSN: 1432-2048
    Keywords: Carnitine palmitoyltransferase (location, function) ; Cotyledon (β-oxidation) ; Microbody ; Mitochondrion ; β-Oxidation ; Pisum (β-oxidation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two sites for β-oxidation of fatty acids in pea (Pisum sativum L.) cotyledons exist. One site is the microbody, the other the mitochondrion. Mitochondrial β-oxidation of fatty acids is carnitine-dependent. The fatty acid permeates the membrane as palmitoylcarnitine which is formed from cytosolic-side palmitoyl-CoA by a carnitine palmitoyltransferase located on the exterior face of the inner mitochondrial membrane as a peripheral protein. A single-gated pore integral membrane translocator is proposed to exchange the palmitoylcarnitine for carnitine or acetylcarnitine across the membrane. An internal (matrix side) carnitine palmitoyltransferase then reforms palmitoyl-CoA which enters β-oxidation and subsequently the tricarboxylic-acid cycle.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Planta 173 (1988), S. 263-266 
    ISSN: 1432-2048
    Keywords: Carnitine ; Carnitine palmitoyltrans-ferase ; Long-chain acylCoA synthetase ; Mitochondrion (outer membrane) ; Mitoplast ; β-Oxidation ; Palmitate ; PalmitoylCoA ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondria from pea (Pisum sativum L.) seeds were separated into two fractions, mitoplasts (intact inner membrane) and the outer-membrane fraction. The mitoplasts only oxidised palmitate in the presence of carnitine and added outermembrane fraction. Mitoplasts were able to oxidise palmitoylCoA in the presence of carnitine and added outer-membrane fraction had no effect on this oxidation. It was concluded that a long-chain acylCoA synthetase (EC 6.2.1.3) was located on the outer membrane and that the activity of this enzyme in assays was more than sufficient to account for any observed rate of O2 uptake during palmitate oxidation by pea mitochondria. The location of carnitine long-chain acyltransferase (carnitine palmitoyl transferase EC 2.3.1.21) would appear to be the mitoplast i.e. the inner mitochondrial membrane, and confirms the previous work at Newcastle.
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  • 4
    facet.materialart.
    Unknown
    In:  Other Sources
    Publication Date: 2019-06-28
    Description: Accuracy at high temperature improved. Furnace heats specimen to experimental temperature, and flash tube raises specimen temperature by small amount and for short time so diffusivity (composite property of heat capacity and conductivity) determined. Specimen mount ensures minimum heat loss during temperature-rise measurement. Measurement temperatures up to 1,000 degrees C realized with fused-quartz light pipe and up to 1,600 degrees C with sapphire light pipe.
    Keywords: PHYSICAL SCIENCES
    Type: NPO-16280 , NASA Tech Briefs (ISSN 0145-319X); 9; 2; P. 76
    Format: text
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  • 5
    Publication Date: 2019-07-12
    Description: Common experimental errors reduced because pulse technique suppresses drifts in thermoelectric measurements. Differential-heat-pulse apparatus measures Seebeck coefficient in semiconductors at temperatures up to 1,900 K. Sample heated to measuring temperature in furnace. Ends of sample then differentially heated a few degrees more by lamps. Differential temperature rise and consequent Seebeck voltage measured via thermocouple leads. Because pulse technique used, errors that often arise from long-term drifts in thermoelectric measurements suppressed. Apparatus works with temperature differences of only few degrees, further increasing accuracy of coefficients obtained.
    Keywords: PHYSICAL SCIENCES
    Type: NPO-16506 , NASA Tech Briefs (ISSN 0145-319X); 10; 1; P. 70
    Format: text
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  • 6
    Publication Date: 2019-07-12
    Description: Apparatus takes measurements and analyzes data automatically. Cylindrical sample is pressed between heater and water-cooled baseplate. Thermocouples at opposite ends of sample provide both temperatures and Seeback voltages. Conveniently shaped samples used, and results calculated by microcomputer and printed out.
    Keywords: PHYSICAL SCIENCES
    Type: NPO-16667 , NASA Tech Briefs (ISSN 0145-319X); 10; 3; P. 57
    Format: text
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