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β-Oxidation enzymes in the mitochondria of Arum and oilseed rape (1990)

Masterson, C. ; Wood, C. ; Thomas, D. R.

Springer

Planta 182 (1990), S. 129-135

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ISSN: 1432-2048

Keywords: Arum ; Brassica (β-oxidation) ; β-Oxidation ; (enzyme location) ; Microbody ; Mitochondrion

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract β-Oxidation enzymes were detected both in the mitochondria and microbodies of Arum maculatum L. spadices and Brassica napus L. seeds. It is apparent that the mitochondrial membrane barrier, which remains intact after sucrose-density-gradient centrifugation, prevents rapid access of acyl-GoA substrates to matrix βoxidation tes. Thus intact mitochondria showed little β-oxidation enzyme activity. Rupturing of the mitochondrial membrane allowed rapid access of acyl CoAs to matrix sites. Consequently, in ruptured mitochondria, high β-oxidation enzyme activities were measured.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00239994

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