ALBERT

Description: We evaluated the relationship between interferon-induced transmembrane protein 1 (IFITM1) expression, epithelial–mesenchymal transition (EMT) signature and angiogenesis in lung adenocarcinoma. Additionally, we examined prognostic significance of IFITM1 according to pTNM stage to confirm that IFITM1 can serve as a complement to the pTNM stage. A total of 141 lung adenocarcinoma specimens were evaluated retrospectively by immunohistochemical staining for IFITM1, EMT markers (e-cadherin, β-catenin, and vimentin), and CD31 to measure microvessel density. IFITM1was expressed in 46.8% of the specimens. IFITM1 expression was significantly correlated with increased microvessel density (P = 0.048). However, IFITM1 expression was not associated with three EMT markers. In a multivariate analysis, IFITM1 was an independent prognostic factor for overall survival in a multivariate analysis (hazard ratio: 2.59, P = 0.01). Online database with data from 720 lung adenocarcinoma patients also revealed a negative prognostic significance of IFITM1 (P 〈 0.001). Furthermore, high IFITM1 expression was significantly correlated with decreased OS rates in each pTNM stage. IFITM1 is significantly correlated with angiogenesis and it may be used as a useful additional prognostic marker to aid pTNM classification.

Description: Correlation of the Paleoproterozoic rocks of the Korean Peninsula and China has been considered to be important for the tectonic evolution of Northeast Asia, yet it is still unclear. Recently considerable new data have been obtained from the Korean Peninsula and China providing a better opportunity to correlate the Paleoproterozoic rocks of the Korean Peninsula and China. Intermediate–P/T metamorphism and post–collisional magmatism have been found to have occurred during ca. 1.95–1.83 Ga not only in the Jiao–Liao–Ji belt on the eastern North China Craton but also in the Nangrim and northern Gyeonggi Massifs within the Korean Peninsula representing that these Paleoproterozoic rocks can be correlated. These events occurred as a result of the collision between the Longgang Block of the eastern North China Craton and the Nangrim Massifs of the Korean Peninsula. On the other hand, the southeastern Gyeonggi Massif underwent an arc–related magmatic and metamorphic events during ca. 1.96–1.91 Ga suggesting that the Paleoproterozoic rocks in southeastern Gyeonggi Massif cannot be correlated with those in northern Gyeonggi Massif. The Yeongnam Massif is unlikely correlated to the Nangrim and northern Gyeonggi Massifs and the eastern North China Craton because arc–related igneous activity occurred during ca. 2.00–1.85 Ga in the Sobaeksan Gneiss Complex in the central to northeastern Yeongnam Massif. The Jirisan Gneiss Complex in the southwestern Yeongnam Massif underwent intermediate–P/T metamorphism that is followed by post–collisional magmatic and low–P/T metamorphic events during ca. 1.92–1.86 Ga and these events may be correlated to those in the eastern Cathaysia Block on the South China Craton. The Paleoproterozoic correlation between the Korean Peninsula and China in this study supports a tectonic model in which the Permo–Triassic Dabie–Sulu continental collision belt in China extends into the Hongseong–Odaesan collision belt in the Gyeonggi Massif within the Korean Peninsula.

Description: Pretreatment of microalgal biomass possessing rigid cell wall is a critical step for enhancing the efficiency of microalgal biorefinery. However, the conventional pretreatment processes suffer the drawbacks of complex processing steps, long processing time, low conversion efficiency and high processing costs. This significantly hinders the industrial applicability of microalgal biorefinery. The innovative electricity-aid pretreatment techniques serve as a promising processing tool to extensively enhance the release of intracellular substances from microalgae. In this review, application of electric field-based techniques and recent advances of using electrical pretreatments on microalgae cell focusing on pulsed electric field, electrolysis, high voltage electrical discharges and moderate electric field are reviewed. In addition, the emerging techniques integrating electrolysis with liquid biphasic flotation process as promising downstream approach is discussed. This review delivers broad knowledge of the present significance of the application of these methods focusing on the development of electric assisted biomolecules extraction from microalgae.

Description: The cause of rapid hydrological changes in the tropical West Pacific during the last deglaciation remains controversial. In order to test whether these changes were triggered by abrupt climate change events in the North Atlantic Ocean, variations in precipitation during the last deglaciation (18–10 ka) were extracted from proxy records of chemical weathering and terrigenous input in the western Philippine Sea (WPS). The evolution of chemical weathering and terrigenous input since 27 ka was reconstructed using the chemical index of alteration (CIA), elemental ratios (K/Al, TOC/TN and Ti/Ca), δ13Corg, terrigenous fraction abundance and flux data from International Marine Global Change Study Program (IMAGES) core MD06-3054 collected on the upper continental slope of eastern Luzon (northern Philippines). Sediment deposited during the Last Glacial Maximum (LGM) shows weathering equal to or slightly greater than Holocene sediment in the WPS. This unusual state of chemical weathering, which is inconsistent with lower air temperatures and decreased precipitation in Luzon during the LGM, may be due to reworking of poorly consolidated sediments on the eastern Luzon continental shelf during the LGM sea-level lowstand. Rapid changes in chemical weathering, characterized by higher intensity during the Heinrich event 1 (H1) and Younger Dryas (YD) and lower intensity during the Bølling-Allerød (B/A), were linked to rapid variations in precipitation in the WPS during the last deglaciation. The higher terrigenous inputs during the LGM relative to those of the Holocene were controlled by sea-level changes rather than precipitation. The terrigenous inputs show a long-term decline during the last deglaciation, punctuated by brief spikes during the H1 and YD related to sea-level rises and rapid precipitation changes in the WPS, respectively. The proxy records of chemical weathering and terrigenous input from eastern Luzon suggest high rainfall during the H1 and YD events, consistent with inferred rainfall patterns based on Fe/Ca records from offshore Mindanao. Rapid precipitation changes in the WPS did not coincide with migrations of the Intertropical Convergence Zone (ITCZ) but, rather, were related to state shifts of the El Niño-Southern Oscillation (ENSO) during the last deglaciation. Based on proxy records and modeling results, we argue that the Atlantic meridional overturning circulation (AMOC) controlled rapid precipitation changes in the tropical West Pacific through zonal shifts of ENSO or meridional migration of the ITCZ during the last deglaciation. Our findings highlight the dominant role of the North Atlantic Ocean in the tropical hydrologic cycle during the last deglaciation.

Description: A major highlight of restoration efforts is to improve the ecological structure and function of the natural ecosystem in the restored habitat. Assessment of restoration success is a crucial component of an optimal ecological management strategy. In studies to determine the restoration success of a transplanted seagrass habitat by assessing trophic recovery, we examined carbon and nitrogen stable isotope ratios of organic matter sources and macrobenthic assemblages in a transplanted eelgrass Zostera marina bed. The eelgrass bed was restored about 2 years after transplantation in a southern coastal bay of Korea, and consequently, the food web structure in the bed was compared with that in a natural reference site. Our results revealed no significant differences in isotopic values of both macrobenthic consumers and their putative food sources between the transplanted and natural seagrass beds. These isotopic similarities in florae and faunae in the two beds suggest a uniformity in food web structure formed by the diversity and availability of resources, and thereby suggest similarities in the resource–consumer relationship. Isotopic niche indices and high dietary overlaps of feeding guilds in the transplanted and natural beds further suggest the transplanted habitat provides similar ecological functions and ecosystem services to its natural counterpart. Collectively, our results suggest the eelgrass transplantation led to successful restoration of a common seagrass bed, with recovery of the functional properties of the food web structure. Finally, our findings support the idea that stable isotope measures can provide a better understanding of the functioning of restored ecosystems, and improve post-transplantation monitoring efforts for the future planning and managing of successful habitat restoration.

Description: The Pb isotope compositions of galena in hydrothermal deposits obtained by drillings from two hydrothermal fields in the middle Okinawa Trough were studied. One of the study fields was the Iheya North field located on a volcanic complex and the other was the Izena field located in the sediment-filled caldera structure. LA-MC-ICP-MS was applied to directly measure Pb isotope compositions in individual galena grains which highlighted variations not only in regional scale, but also in microscopic scale so that changes of Pb isotope compositions within a galena grain can be tracked. Homogeneous Pb isotope compositions were found within the same hydrothermal site, irrespective of the mineral assemblage, texture and sampling depth beneath the seafloor. In contrast, the isotope compositions varied significantly between the two hydrothermal fields. The Pb isotope composition from the Iheya North field was isotopically close to the volcanic rocks of the Okinawa Trough, whereas that from the Izena field was more radiogenic with values intermediate between sediments and volcanic rocks of the Okinawa Trough. Within the Iheya North field, intra-field variation of Pb isotope compositions was recognized between two active sites 2.5 km apart from each other. The intra-field variation was recognized also in the Izena field. The sub-seafloor massive sulfide layer has a more sediment-like Pb isotope composition, compared to the inactive sulfide mound. These results illustrate that the Pb-mineralizing hydrothermal fluids originate from their local host rocks with/without sediment and that the isotope compositions of the galena grains reflect their metal sources; either the volcanic rocks and/or the sediments via water-rock interactions.

Description: The Muju area, located on the north–central margin of the Yeongnam Massif, mainly consists of Precambrian orthogneisses (granitic, leucogranitic, augen and dioritic gneisses) with minor migmatite. Zircon U–Pb dating indicates that the protoliths of the orthogneisses intruded at ca. 2.00–1.97 Ga and were metamorphosed at ca. 1.87–1.86 Ga. Magmatic zircon grains within the orthogneisses have positive to negative εHf(t) values (−7.63 to +3.3) and a Neoarchean two-stage model age (TDM2 = 2.78 Ga), indicating that the protoliths of most of the orthogneisses may have been derived from Archean crustal material. The results of geochemical analysis indicate that the protoliths of the orthogneisses formed by partial melting of metagraywacke and mafic igneous rocks in an arc-related tectonic setting. The intrusion ages and geochemical data of the Paleoproterozoic orthogneisses in the study area match well with those of Paleoproterozoic (ca. 2.00–1.97 Ga) orthogneisses in the northeastern Yeongnam Massif, indicating the presence of regional Paleoproterozoic subduction zones along the northern margin of the Yeongnam Massif at ca. 2.00–1.97 Ga. Meanwhile, ca. 2.00–1.97 Ga subduction-related magmatism has not been reported from the northern Gyeonggi and Nangrim Massifs in the Korean Peninsula or the Jiao–Liao–Ji belt in the eastern North China Craton, indicating that the Yeongnam Massif may not be correlatable with the northern Gyeonggi and Nangrim Massifs or the Jiao–Liao–Ji belt. The Yeongnam Massif may be correlated with the Cathaysia Block in the South China Craton and may have been located near Laurentia and the Siberian Craton within the Columbia supercontinent.

Description: Egg production, egg viability and fecal pellet production were determined for individual Acartia omorii, which were fed diets of two species of diatoms (Skeletonema costatum and Phaeodactylum tricornutum) and three species of dinoflagellates (Scrippsiella trochoidea, Heterocapsa triquetra and Cochlodinium polykrikoides). Diets were analyzed for fatty acid content as an indicator of food quality. Depending on the diet, egg production of A. omorii varied over time, diminishing with some diets (S. trochoidea, C. polykrikoides, P. tricornutum). This rate of reduction was much more rapid for a diet of C. polykrikoides, which caused egg production to decrease to ca. 2.4 eggs f−1 d−1 in only four days. As for all diets, egg viability was high at the beginning but with the C. polykrikoides and P. tricornutum diets, it rapidly decreased with time. Fecal pellet production also varied with time, depending on the diet. Egg production rate was closely correlated with fecal pellet production. There was no direct relationship between egg viability and egg production rate, but both egg production and viability were affected by the nutritional quality of food. Egg viability was also highly dependent on the composition of fatty acids in the eggs. Egg viability showed positive correlation with the ratio of ω3:ω6 groups among egg fatty acids, and negative correlation with the ratio of 20:5 (n−3) : 22:6 (n−3). While comparing several diets, egg production rate was higher on diets (H. triquetra and S. trochoidea) containing ample amounts of essential fatty acids such as 18:4 (n−3) and 22:6 (n−3). The results suggest that fertility of A. omorii was dependent upon the quality of the food, and dinoflagellate diets, with the exception of C. polykrikoides, were preferable to diatom diets.