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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Weed research 28 (1988), S. 0 
    ISSN: 1365-3180
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Treatment of both tolerant and susceptible species with dinitroaniline herbicides results in root swelling typical of mitotic disrupters, however, no root swelling was observed in carrots treated with saturated solutions of all the dinitroaniline herbicides tested, with the exception of oryzalin treatments at ≥ 10−5 M. These levels of treatment are 100–1000 times the levels that caused root-tip swelling, even in tolerant plants. This properly classifies carrot as dinitroaniline-resistant rather than merely tolerant. Cross-resistance was noted to the structurally related mitotic disrupter, hexanitrodiphenylamine, and to the structurally unrelated herbicide, amiprophos-methyl. No resistance was noted to a number of other mitotic-disrupting herbicides. Both immunofluorescence and electron microscopy indicate that the microtubules of carrot roots were unaffected by dinitroaniline treatment. All organized microtubule configurations (cortical, pre-prophase bands, spindle, and phragmoplast) were observed in the treated material.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 86 (1987), S. 533-535 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An auto-antibody from human serum of patients with the autoimmune disease scleroderma was used to localize the nucleolus in meristematic cells of onion and soybean roots using indirect immunofluorescence microscopy. Similar lots of antiserum recognized a single 34 kD, nucleolar protein, fibrillarin, in a variety of animal cells (Ochs, et al. 1984, 1985). In both plants, antibody linked fluorescence is associated with the one to several nucleoli present in the interphase nucleus. The fluorescence becomes diffuse around condensing prophase chromosomes and becomes more diffused at metaphase with slightly more intense fluorescence surrounding the chromosomes. At anaphase-telophase the fluorescence is localized in dense areas within the chromosomes, presumably representing prenucleolar bodies which will form the interphase nucleoli of the daughter nuclei. This antiserum provides a new, valuable tool for the study of the nucleolus and the highly conversed nucleolar antigen(s) that it recognizes.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 87 (1987), S. 129-137 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Maize root tip cells were examined for the distribution of actin microfilaments in various cell types and to determine the effects of microfilament disrupters. Fluorescence microscopy on fixed, stabilized, squashed cells using the F-actin specific probe, rhodamine-labelled phalloidin, allowed for a three-dimensional visualization of actin microfilaments. Microfilaments were observed as long, meandering structures in root cap cells and meristematic cells, while those in immature vascular parenchyma were abundant in the thin band of cytoplasm and were long and less curved. By modifying standard electron microscopic fixation procedures, microfilaments in plant cells could be easily detected in all cell types. Treatment with cytochalasin B, cytochalasin D and lead acetate, compounds that interfere with microfilament related processes, re-organized the microfilaments into abnormal crossed and highly condensed masses. All the treatments affected not only the microfilaments but also the accumulation of secretory vesicles. The vivid demonstration of the effects of all of these microfilament disrupters on the number and size of Golgi vesicles indicates that these vesicles may depend on microfilaments for intracellular movement.
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  • 4
    Publication Date: 1987-01-01
    Print ISSN: 0018-2222
    Electronic ISSN: 1432-119X
    Topics: Biology , Medicine
    Published by Springer
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  • 5
    Publication Date: 1987-01-01
    Print ISSN: 0018-2222
    Electronic ISSN: 1432-119X
    Topics: Biology , Medicine
    Published by Springer
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  • 6
    Publication Date: 2011-02-15
    Description: The Cloud-Aerosol Lidar with Orthogonal Polarization (CALIOP) instrument on the Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observations (CALIPSO) spacecraft has provided global, high-resolution vertical profiles of aerosols and clouds since it became operational on 13 June 2006. On 14 June 2006, the NASA Langley Research Center (LaRC) High Spectral Resolution Lidar (HSRL) was deployed aboard the NASA Langley B-200 aircraft for the first of a series of 86 underflights of the CALIPSO satellite to provide validation measurements for the CALIOP data products. To better assess the range of conditions under which CALIOP data products are produced, these validation flights were conducted under both daytime and nighttime lighting conditions, in multiple seasons, and over a large range of latitudes and aerosol and cloud conditions. This paper presents a quantitative assessment of the CALIOP 532 nm calibration (through the 532 nm total attenuated backscatter) using internally calibrated airborne HSRL underflight data and is the most extensive study of CALIOP 532 nm calibration. Results show that HSRL and CALIOP 532 nm total attenuated backscatter agree on average within 2.7% ± 2.1% (CALIOP lower) at night and within 2.9% ± 3.9% (CALIOP lower) during the day, demonstrating the accuracy of the CALIOP 532 nm calibration algorithms. Additionally, comparisons with HSRL show consistency of the CALIOP calibration before and after the laser switch in 2009 as well as improvements in the daytime version 3.01 calibration scheme compared with the version 2 calibration scheme. Potential biases and uncertainties in the methodology relevant to validating satellite lidar measurements with an airborne lidar system are discussed and found to be less than 4.5% ± 3.2% for this validation effort with HSRL. Results from this study are also compared with prior assessments of the CALIOP 532 nm attenuated backscatter calibration.
    Print ISSN: 1680-7316
    Electronic ISSN: 1680-7324
    Topics: Geosciences
    Published by Copernicus on behalf of European Geosciences Union.
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  • 7
    Publication Date: 2011-04-29
    Description: The Cloud Aerosol LIdar with Orthogonal Polarization (CALIOP), on board the CALIPSO platform, has measured profiles of total attenuated backscatter coefficient (level 1 products) since June 2006. CALIOP's level 2 products, such as the aerosol backscatter and extinction coefficient profiles, are retrieved using a complex succession of automated algorithms. The goal of this study is to help identify potential shortcomings in the CALIOP version 2 level 2 aerosol extinction product and to illustrate some of the motivation for the changes that have been introduced in the next version of CALIOP data (version 3, released in June 2010). To help illustrate the potential factors contributing to the uncertainty of the CALIOP aerosol extinction retrieval, we focus on a one-day, multi-instrument, multiplatform comparison study during the CALIPSO and Twilight Zone (CATZ) validation campaign on 4 August 2007. On that day, we observe a consistency in the Aerosol Optical Depth (AOD) values recorded by four different instruments (i.e. space-borne MODerate Imaging Spectroradiometer, MODIS: 0.67 and POLarization and Directionality of Earth's Reflectances, POLDER: 0.58, airborne High Spectral Resolution Lidar, HSRL: 0.52 and ground-based AErosol RObotic NETwork, AERONET: 0.48 to 0.73) while CALIOP AOD is a factor of two lower (0.32 at 532 nm). This case study illustrates the following potential sources of uncertainty in the CALIOP AOD: (i) CALIOP's low signal-to-noise ratio (SNR) leading to the misclassification and/or lack of aerosol layer identification, especially close to the Earth's surface; (ii) the cloud contamination of CALIOP version 2 aerosol backscatter and extinction profiles; (iii) potentially erroneous assumptions of the aerosol extinction-to-backscatter ratio (Sa) used in CALIOP's extinction retrievals; and (iv) calibration coefficient biases in the CALIOP daytime attenuated backscatter coefficient profiles. The use of version 3 CALIOP extinction retrieval for our case study seems to partially fix factor (i) although the aerosol retrieved by CALIOP is still somewhat lower than the profile measured by HSRL; the cloud contamination (ii) appears to be corrected; no particular change is apparent in the observation-based CALIOP Sa value (iii). Our case study also showed very little difference in version 2 and version 3 CALIOP attenuated backscatter coefficient profiles, illustrating a minor change in the calibration scheme (iv).
    Print ISSN: 1680-7316
    Electronic ISSN: 1680-7324
    Topics: Geosciences
    Published by Copernicus on behalf of European Geosciences Union.
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  • 8
    Publication Date: 2013-03-25
    Description: The CALIOP lidar, carried on the CALIPSO satellite, has been acquiring global atmospheric profiles since June 2006. This dataset now offers the opportunity to characterize the global 3-D distribution of aerosol as well as seasonal and interannual variations, and confront aerosol models with observations in a way that has not been possible before. With that goal in mind, a monthly global gridded dataset of daytime and nighttime aerosol extinction profiles has been constructed, available as a Level 3 aerosol product. Averaged aerosol profiles for cloud-free and all-sky conditions are reported separately. This 6-yr dataset characterizes the global 3-dimensional distribution of tropospheric aerosol. Vertical distributions are seen to vary with season, as both source strengths and transport mechanisms vary. In most regions, clear-sky and all-sky mean aerosol profiles are found to be quite similar, implying a lack of correlation between high semi-transparent cloud and aerosol in the lower troposphere. An initial evaluation of the accuracy of the aerosol extinction profiles is presented. Detection limitations and the representivity of aerosol profiles in the upper troposphere are of particular concern. While results are preliminary, we present evidence that the monthly-mean CALIOP aerosol profiles provide quantitative characterization of elevated aerosol layers in major transport pathways. Aerosol extinction in the free troposphere in clean conditions, where the true aerosol extinction is typically 0.001 km−1 or less, is generally underestimated, however. The work described here forms an initial global 3-D aerosol climatology which we plan to extend and improve over time.
    Print ISSN: 1680-7316
    Electronic ISSN: 1680-7324
    Topics: Geosciences
    Published by Copernicus on behalf of European Geosciences Union.
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  • 9
    Publication Date: 2010-08-09
    Description: The Cloud-Aerosol Lidar with Orthogonal Polarization (CALIOP) is carried on the CALIPSO satellite and has acquired global aerosol profiles since June 2006. CALIPSO is flown in formation with the Aqua satellite as part of the A-train satellite constellation, so that a large number of coincident aerosol observations are available from CALIOP and the MODIS-Aqua instrument. This study compares column aerosol optical depth at 0.532 μm derived from CALIOP aerosol profiles with MODIS-Aqua 0.55 μm aerosol optical depth over the period June 2006 through August 2008. The study is based on the CALIOP Version 2 Aerosol Layer Product and MODIS Collection 5. While CALIOP is first and foremost a profiling instrument, this comparison of column aerosol optical depth provides insight into quality of CALIOP aerosol data. It is found that daytime aerosol optical depth from the CALIOP Version 2 product has a small global mean bias relative to MODIS Collection 5. Regional biases, of both signs, are larger and biases are seen to vary somewhat with season. In northern mid-latitudes, aerosol optical depth from CALIOP is lower, on average, than from MODIS. This may be partly due to a latitude-dependent calibration error in Version 2 CALIOP Level 1 daytime 0.532 μm profiles. This comparison of CALIOP and MODIS also provides insight into possible biases in the MODIS aerosol optical depth product due to cloud masking and errors in modeling land surface reflectance.
    Electronic ISSN: 1867-8610
    Topics: Geosciences
    Published by Copernicus on behalf of European Geosciences Union.
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  • 10
    Publication Date: 2015-02-25
    Description: This paper provides a detailed evaluation of cloud absorption optical depths retrieved at 12.05 μm and comparisons to extinction optical depths retrieved at 0.532 μm from perfectly co-located observations of single-layered semi-transparent cirrus over ocean made by the Imaging Infrared Radiometer (IIR) and the Cloud and Aerosol Lidar with Orthogonal Polarization (CALIOP) flying on-board the CALIPSO (Cloud-Aerosol Lidar and Infrared Pathfinder Satellite Observations) satellite. The blackbody radiance taken in the IIR Version 3 algorithm is evaluated, and IIR retrievals are corrected accordingly. IIR infrared absorption optical depths are then compared to CALIOP visible extinction optical depths when the latter can be directly derived from the measured apparent 2-way transmittance through the cloud. Numerical simulations and IIR retrievals of ice crystal sizes suggest that the ratios of CALIOP extinction and IIR absorption optical depths should remain roughly constant with respect to temperature. Instead, these ratios are found to increase quasi-linearly by about 40% as the temperature at the layer centroid altitude decreases from 240 to 200 K. This behavior is explained by variations of the multiple scattering factor ηT to be applied to correct the measured transmittance, which is taken equal to 0.6 in the CALIOP Version 3 algorithm, and which is found here to vary with temperature (and hence cloud particle size) from ηT = 0.8 at 200 K to ηT = 0.5 at 240 K for clouds with optical depth larger than 0.3. The revised parameterization of ηT introduces a concomitant temperature dependence in the simultaneously derived CALIOP lidar ratios that is consistent with observed changes in CALIOP depolarization ratios and particle habits derived from IIR measurements.
    Electronic ISSN: 1867-8610
    Topics: Geosciences
    Published by Copernicus on behalf of European Geosciences Union.
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