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An autoimmune antibody from scleroderma patients recognizes a component of the plant cell nucleolus

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Summary

An auto-antibody from human serum of patients with the autoimmune disease scleroderma was used to localize the nucleolus in meristematic cells of onion and soybean roots using indirect immunofluorescence microscopy. Similar lots of antiserum recognized a single 34 kD, nucleolar protein, fibrillarin, in a variety of animal cells (Ochs, et al. 1984, 1985). In both plants, antibody linked fluorescence is associated with the one to several nucleoli present in the interphase nucleus. The fluorescence becomes diffuse around condensing prophase chromosomes and becomes more diffused at metaphase with slightly more intense fluorescence surrounding the chromosomes. At anaphase-telophase the fluorescence is localized in dense areas within the chromosomes, presumably representing prenucleolar bodies which will form the interphase nucleoli of the daughter nuclei. This antiserum provides a new, valuable tool for the study of the nucleolus and the highly conversed nucleolar antigen(s) that it recognizes.

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References

  • Beck JS, Anderson JR, McElhinney AJ, Rowell NR (1962) Antinucleolar antibodies. Lancet 2:575–577

    Google Scholar 

  • Bernstein RW, Steigerwald JC, Tan EM (1982) Association of antinuclear and antinucleolar antibodies in systemic sclerosis. Clin Exp Immunol 48:43–51

    Google Scholar 

  • Clayton L, Black CM, Lloyd CW (1985) Microtubule nucleating sites in higher plant, cells identified by an auto-antibody against pericentrcolar material. J Cell Biol 101:319–324

    Google Scholar 

  • Coleman AW, Maguire MJ, Coleman JR (1981) Mithramycin- and 4′-6-diamidino-2-phenylindole, (DAPI)-DNA staining for fluorescence microspectrophotometric measurement of DNA in nuclei, plastids, and virus particles. J Histochem Cytochem 29:959–968

    Google Scholar 

  • Hernandez-Verdun D, Bouteille M, Ege T, Ringertz NR (1979) Fine structure of nucleoli in micronucleated cells. Exp Cell Res 124:223–235

    Google Scholar 

  • Lischwe MA, Ochs RL, Reddy R, Cook RG, Yeoman LC, Tan EM, Reichlin M, Busch H (1985) Purification and partial characterization of a nucleolar seleroderma antigen (Mr=34,000; pI, 8.5) rich in NG, NG-dimethylarginine. J Biol Chem 260:14304–14310

    Google Scholar 

  • Lloyd CW, Clayton L, Dawson PJ, Doonan JH, Hulme JS, Roberts IN, Wells B (1985) The cytoskeleton underlying side walls and cross walls in plants: molecules and macromolecular assemblies. J Cell Sci (Suppl) 2:143–155

    Google Scholar 

  • McLean G, Hightower R, Meagher R (1986) Observation, of 62 kD actin-related proteins in soybean. Plant Physiol 805:79

    Google Scholar 

  • Miyawaki S, Ritchie RF (1973) Nucleolar antigen specific for antinucleolar antibody in the sera of patients with systemic rheumatic disease. Arthritis Rheumatism 16:726–736

    Google Scholar 

  • Ochs RL, Spohn WH, Lischwe MA (1984) Autoimmuneserum from a patient with scleroderma recognizes a new protein of the nucleolus: fibrillarin. J Cell Biol 99:12a

    Google Scholar 

  • Ochs RL, Lischwe MA, Spohn WH, Busche H (1985) Fibrillarin: a new protein of the nucleolus identified by autoimmune sera. Biol Cell 54:123–134

    Google Scholar 

  • Pinnas JL, Northway JD, Tan EM (1973) Antinucleolar antibodies in human sera. J Immunol 111:996–1004

    Google Scholar 

  • Ritchie RF (1969) Nucleolar structure by immunologic techniques. Cancer Res 29:267–269

    Google Scholar 

  • Ritchie RF (1970) Antinucleolar antibodies. Their frequency and diagnostic association. N Engl J Med 282:1174–1178

    Google Scholar 

  • Sato S, Shigematsu T (1985) Light and electron microscopic study on the behavior of the nucleolar material during mitosis in root tip meristem of Vicia faba. Caryologia 38:103–120

    Google Scholar 

  • Steele RE, Thomas PS, Reeder RH (1984) Anucleolate frog embryos contain ribosomal DNA sequences and a nucleolar antigen. Dev Biol 102:409–416

    Google Scholar 

  • Stetler DA, Rose KM, Wenger ME, Berlin CM, Jacob ST (1982) Antibodies to distinct polypeptides of RNA polymerase I in sera from patients with rheumatic autoimmune disease. Proc Natl Acad Sci USA 79:7499–7503

    Google Scholar 

  • Tuffanelli DL, McKeon F, Kleinsmith DM, Burnham TK, Kirschner M (1983) Anticentromere and anticentriole antibodies in the scleroderma spectrum. Arch Dermatol 119:560–566

    Google Scholar 

  • Wick SM (1985) Immunofluorescence microscopy of tubulin and microtubules arrays in plant cells. III. Transition between mitotic/cytokinetic and interphase microtubule arrays. Cell Biol Int Rep 9:357–371

    Google Scholar 

  • Wick SM, Seagull RW, Osborn M, Webber K, Gunning BES (1981) Immunofluorescence microscopy of organized microtubule arrays in structurally stabilized meristematic plant cells. J Cell Biol 89:635–690

    Google Scholar 

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Authors and Affiliations

  1. Southern Weed Science Laboratory, USDA, ARS, P.O. Box 350, 38776, Stoneville, MS, USA

    M. A. Vaughan

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  1. M. A. Vaughan
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Vaughan, M.A. An autoimmune antibody from scleroderma patients recognizes a component of the plant cell nucleolus. Histochemistry 86, 533–535 (1987). https://doi.org/10.1007/BF00500629

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  • DOI: https://doi.org/10.1007/BF00500629

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