Publication Date:
2015-10-10
Description:
Cellulose biosynthesis in plant secondary cell walls forms the basis of vascular development in land plants, with xylem tissues constituting the vast majority of terrestrial biomass. We used plant lines that contained an inducible master transcription factor controlling xylem cell fate to quantitatively image fluorescently tagged cellulose synthase enzymes during cellulose deposition in living protoxylem cells. The formation of secondary cell wall thickenings was associated with a redistribution and enrichment of CESA7-containing cellulose synthase complexes (CSCs) into narrow membrane domains. The velocities of secondary cell wall-specific CSCs were faster than those of primary cell wall CSCs during abundant cellulose production. Dynamic intracellular of endomembranes, in combination with increased velocity and high density of CSCs, enables cellulose to be synthesized rapidly in secondary cell walls.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Watanabe, Y -- Meents, M J -- McDonnell, L M -- Barkwill, S -- Sampathkumar, A -- Cartwright, H N -- Demura, T -- Ehrhardt, D W -- Samuels, A L -- Mansfield, S D -- New York, N.Y. -- Science. 2015 Oct 9;350(6257):198-203. doi: 10.1126/science.aac7446.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Botany, University of British Columbia, Vancouver, BC, Canada. Department of Wood Science, University of British Columbia, Vancouver, BC, Canada. ; Department of Wood Science, University of British Columbia, Vancouver, BC, Canada. ; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, USA. ; Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA. ; Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan. ; Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA. Department of Biological Sciences, Stanford University, Stanford, CA, USA. ; Department of Botany, University of British Columbia, Vancouver, BC, Canada. annelacey.samuels@botany.ubc.ca shawn.mansfield@ubc.ca. ; Department of Wood Science, University of British Columbia, Vancouver, BC, Canada. annelacey.samuels@botany.ubc.ca shawn.mansfield@ubc.ca.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26450210" target="_blank"〉PubMed〈/a〉
Keywords:
Arabidopsis/*enzymology/growth & development
;
Arabidopsis Proteins/*analysis/chemistry/metabolism
;
Bacterial Proteins/analysis
;
Cell Wall/*enzymology/ultrastructure
;
Cellulose/biosynthesis/metabolism
;
Fluorescence
;
Glucosyltransferases/*analysis/chemistry/metabolism
;
Golgi Apparatus/enzymology
;
Luminescent Proteins/analysis
;
Microtubules/enzymology
;
Protein Transport
;
Xylem/enzymology/growth & development
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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