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  • 1
    Publication Date: 2014-10-07
    Description: Crystal Growth & Design DOI: 10.1021/cg500813q
    Print ISSN: 1528-7483
    Electronic ISSN: 1528-7505
    Topics: Chemistry and Pharmacology , Geosciences , Physics
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  • 2
    Publication Date: 2014-01-18
    Description: Transcription factors (TFs) are key players in evolution. Changes affecting their function can yield novel life forms but may also have deleterious effects. Consequently, gene duplication events that release one gene copy from selective pressure are thought to be the common mechanism by which TFs acquire new activities. Here, we show that LEAFY, a major regulator of flower development and cell division in land plants, underwent changes to its DNA binding specificity, even though plant genomes generally contain a single copy of the LEAFY gene. We examined how these changes occurred at the structural level and identify an intermediate LEAFY form in hornworts that appears to adopt all different specificities. This promiscuous intermediate could have smoothed the evolutionary transitions, thereby allowing LEAFY to evolve new binding specificities while remaining a single-copy gene.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Sayou, Camille -- Monniaux, Marie -- Nanao, Max H -- Moyroud, Edwige -- Brockington, Samuel F -- Thevenon, Emmanuel -- Chahtane, Hicham -- Warthmann, Norman -- Melkonian, Michael -- Zhang, Yong -- Wong, Gane Ka-Shu -- Weigel, Detlef -- Parcy, Francois -- Dumas, Renaud -- New York, N.Y. -- Science. 2014 Feb 7;343(6171):645-8. doi: 10.1126/science.1248229. Epub 2014 Jan 16.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉CNRS, Laboratoire de Physiologie Cellulaire et Vegetale (LPCV), UMR 5168, 38054 Grenoble, France.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24436181" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Arabidopsis Proteins/chemistry/classification/genetics ; DNA, Plant/*chemistry ; DNA-Binding Proteins/*chemistry/classification/*genetics ; Electrophoretic Mobility Shift Assay ; *Evolution, Molecular ; Gene Dosage ; Molecular Sequence Data ; Mutation ; Phylogeny ; Plant Proteins/*chemistry/classification/*genetics ; Protein Binding/genetics ; Protein Structure, Tertiary ; Species Specificity ; Transcription Factors/chemistry/classification/genetics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
    Publication Date: 2015-02-07
    Description: Brunkard et al. propose that the identification of novel LEAFY sequences contradicts our model of evolution through promiscuous intermediates. Based on the debate surrounding land plant phylogeny and on our analysis of these interesting novel sequences, we explain why there is no solid evidence to disprove our model.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Brockington, Samuel F -- Moyroud, Edwige -- Sayou, Camille -- Monniaux, Marie -- Nanao, Max H -- Thevenon, Emmanuel -- Chahtane, Hicham -- Warthmann, Norman -- Melkonian, Michael -- Zhang, Yong -- Wong, Gane Ka-Shu -- Weigel, Detlef -- Dumas, Renaud -- Parcy, Francois -- New York, N.Y. -- Science. 2015 Feb 6;347(6222):621. doi: 10.1126/science.1256011.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK. ; Wellcome Trust Center for Cell Biology, Michael Swann Building 5.1, King's Buildings. Edinburgh, EH9 3JR, UK. ; Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linne Weg 10, 50829, Koln, Germany. ; European Molecular Biology Laboratory (EMBL), 6 Rue Jules Horowitz, BP 181, 38042 Grenoble, France. Unit of Virus Host-Cell Interactions, Universite Grenoble Alpes (UGA), Centre National de la Recherche Scientifique (CNRS), EMBL, UMI 3265, 6 Rue Jules Horowitz, 38042 Grenoble Cedex 9, France. francois.parcy@cea.fr mnanao@embl.fr. ; CNRS, Laboratoire de Physiologie Cellulaire et Vegetale (LPCV), UMR 5168, 38054 Grenoble, France. UGA, LPCV, F-38054 Grenoble, France. Commissariat a l'energie atomique et aux energies alternatives, Direction des Sciences du Vivant, Institut de Recherches en Technologies et Sciences pour le Vivant, LPCV, F-38054 Grenoble, France. Institut National de la Recherche Agronomique, LPCV, F-38054 Grenoble, France. ; Research School of Biology, The Australian National University, Acton, ACT 0200, Australia. ; Botanisches Institut, Lehrstuhl I, Universitat zu Koln, Biozentrum Koln, Zulpicher Strasse 47b, 50674 Koln, Germany. ; Beijing Genomics Institute, Shenzhen, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China. ; Beijing Genomics Institute, Shenzhen, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China Department of Biological Sciences, Department of Medicine, University of Alberta, Edmonton AB, T6G 2E9, Canada. ; Department of Molecular Biology, Max Planck Institute for Developmental Biology, 72076 Tubingen, Germany. ; CNRS, Laboratoire de Physiologie Cellulaire et Vegetale (LPCV), UMR 5168, 38054 Grenoble, France. UGA, LPCV, F-38054 Grenoble, France. Commissariat a l'energie atomique et aux energies alternatives, Direction des Sciences du Vivant, Institut de Recherches en Technologies et Sciences pour le Vivant, LPCV, F-38054 Grenoble, France. Institut National de la Recherche Agronomique, LPCV, F-38054 Grenoble, France. francois.parcy@cea.fr mnanao@embl.fr.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/25657241" target="_blank"〉PubMed〈/a〉
    Keywords: DNA, Plant/*chemistry ; DNA-Binding Proteins/*chemistry/*genetics ; *Evolution, Molecular ; Plant Proteins/*chemistry/*genetics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biosystems 12 (1980), S. 85-104 
    ISSN: 0303-2647
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Journal of Ultrasructure Research 72 (1980), S. 90-102 
    ISSN: 0022-5320
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 0014-5793
    Keywords: Eyespot ; Green algae ; Photoreceptive organelle ; Phototaxis ; Retinal
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    FEBS Letters 180 (1985), S. 253-258 
    ISSN: 0014-5793
    Keywords: Ca^2^+ ; Chloroplast ; Electrogenic uptake ; Spinach ; Uniport
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Cell Biology International Reports 6 (1982), S. 269-277 
    ISSN: 0309-1651
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-2048
    Keywords: Calcium channel blocker ; Calcium flux ; Chloroplast (Ca2+ flux) ; Membrane potential ; NAD+level ; Spinacia (chloroplast, calcium)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Calcium fluxes across the envelope of intact spinach chloroplasts (Spinacia oleracea L.) in the light and in the dark were investigated using the metallochromic indicator arsenazo III. Light induces Ca2+ influx into chloroplasts. The action spectrum of light-induced Ca2+ influx and the inhibitory effect of 3-(3',4'-dichlorophenyl)-1,1-dimethylurea (DCMU) indicate an involement of photosynthetic electron transport in this process. The driving force for light-induced Ca2+ influx is most likely a change in the membrane potential component of the proton motive force. This was demonstrated by the use of agents modifying the membrane potential (lipophilic cations, ionophores, different KCl concentrations). The activation energy of the observed Ca2+ influx is about 92 kJ mol-1. Verapamil and nifedipine, two Ca2+-channel blockers, have no inhibitory effect on light-induced Ca2+ influx, but enhance ferricyanide-dependent oxygen evolution. Inhibition of Ca2+ influx by ruthenium red reduces the light-dependent decrease in stromal NAD+ level.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1438-3888
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Flagellar regeneration after experimental amputation was studied in synchronized axenic cultures of the scaly green flagellateTetraselmis striata (Prasinophyceae). After removal of flagella by mechanical shearing, 95% of the cells regrow all four flagella (incl. the scaly covering) to nearly full length with a linear velocity of 50 nm/min under standard conditions. Flagellar regeneration is independent of photosynthesis (no effect of DCMU; the same regeneration rate in the light or in the dark), but depends on de novo protein synthesis: cycloheximide at a low concentration (0.35 μM) blocks flagellar regeneration reversibly. No pool of flagellar precursors appears to be present throughout the flagellated phase of the cell cycle. A transient pool of flagellar precursors, sufficient to generate 2.5 μm of flagellar length, however, develops during flagellar regeneration. Tunicamycin (2 μg/ml) inhibits flagellar regeneration only after a second flagellar amputation, when flagella reach only one third the length of the control. Flagellar regeneration inT. striata differs considerably from that ofChlamydomonas reinhardtii and represents an excellent model system for the study of synchronous Golgi apparatus (GA) activation, and transport and exocytosis of GA-derived macromolecules (scales).
    Type of Medium: Electronic Resource
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