Publication Date:
2012-09-01
Description:
Identification of the causal genes that control complex trait variation remains challenging, limiting our appreciation of the evolutionary processes that influence polymorphisms in nature. We cloned a quantitative trait locus that controls plant defensive chemistry, damage by insect herbivores, survival, and reproduction in the natural environments where this polymorphism evolved. These ecological effects are driven by duplications in the BCMA (branched-chain methionine allocation) loci controlling this variation and by two selectively favored amino acid changes in the glucosinolate-biosynthetic cytochrome P450 proteins that they encode. These changes cause a gain of novel enzyme function, modulated by allelic differences in catalytic rate and gene copy number. Ecological interactions in diverse environments likely contribute to the widespread polymorphism of this biochemical function.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872477/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3872477/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Prasad, Kasavajhala V S K -- Song, Bao-Hua -- Olson-Manning, Carrie -- Anderson, Jill T -- Lee, Cheng-Ruei -- Schranz, M Eric -- Windsor, Aaron J -- Clauss, Maria J -- Manzaneda, Antonio J -- Naqvi, Ibtehaj -- Reichelt, Michael -- Gershenzon, Jonathan -- Rupasinghe, Sanjeewa G -- Schuler, Mary A -- Mitchell-Olds, Thomas -- R01 GM086496/GM/NIGMS NIH HHS/ -- R01-GM079530/GM/NIGMS NIH HHS/ -- R01-GM086496/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2012 Aug 31;337(6098):1081-4. doi: 10.1126/science.1221636.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biology, Institute for Genome Sciences and Policy, Duke University, Durham, NC 27708, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22936775" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
Amino Acid Sequence
;
Amino Acid Substitution
;
Arabidopsis/genetics/metabolism/parasitology
;
*Brassicaceae/genetics/metabolism/parasitology
;
Cytochrome P-450 Enzyme System/*genetics
;
Gene Dosage
;
Gene-Environment Interaction
;
Glucosinolates/biosynthesis/*genetics
;
Herbivory/physiology
;
Methionine/genetics/metabolism
;
Molecular Sequence Data
;
Plant Leaves/genetics/metabolism/parasitology
;
Plants, Genetically Modified/genetics/metabolism/parasitology
;
Polymorphism, Genetic
;
*Quantitative Trait Loci
;
*Quantitative Trait, Heritable
;
*Selection, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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