Abstract
Transcription factors of the nuclear factor-kappaB/rel (NF-kappaB) family may be important in cell survival by regulating unidentified, anti-apoptotic genes. One such gene that protects cells from apoptosis induced by Fas or tumor necrosis factor type alpha (TNF), IEX-1L, is described here. Its transcription induced by TNF was decreased in cells with defective NF-kappaB activation, rendering them sensitive to TNF-induced apoptosis, which was abolished by transfection with IEX-1L. In support, overexpression of antisense IEX-1L partially blocked TNF-induced expression of IEX-1L and sensitized normal cells to killing. This study demonstrates a key role of IEX-1L in cellular resistance to TNF-induced apoptosis.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Apoptosis / genetics
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Apoptosis / physiology*
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Apoptosis Regulatory Proteins
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Cell Line
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Cell Survival
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Cloning, Molecular
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DNA, Antisense / genetics
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Gene Expression Regulation
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Genetic Vectors
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Humans
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Immediate-Early Proteins / genetics
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Immediate-Early Proteins / physiology*
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Jurkat Cells
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / physiology*
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Membrane Proteins
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Mice
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NF-kappa B / physiology*
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Neoplasm Proteins*
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Transfection
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Tumor Necrosis Factor-alpha / physiology
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fas Receptor / physiology
Substances
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Apoptosis Regulatory Proteins
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DNA, Antisense
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IER3 protein, human
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Immediate-Early Proteins
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Membrane Glycoproteins
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Membrane Proteins
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NF-kappa B
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Neoplasm Proteins
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Tumor Necrosis Factor-alpha
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fas Receptor
Associated data
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GENBANK/AF039067
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GENBANK/AF071596