Publication Date:
2006-06-17
Description:
In contrast to naive T cells that recognize short antigen-derived peptides displayed by specialized antigen-presenting cells, immunoglobulin receptors of B lymphocytes primarily recognize intact proteins. How and where within a lymph node such unprocessed antigens become available for naive B cell recognition is not clear. We used two-photon intravital imaging to show that, after exiting high-endothelial venules and before entry into lymph node follicles, B cells survey locally concentrated dendritic cells. Engagement of the B cell receptor by the dendritic cell (DC)-associated antigen leads to lymphocyte calcium signaling, migration arrest, antigen acquisition, and extrafollicular accumulation. These findings suggest a possible role for antigen-specific B-DC interactions in promoting T cell-dependent antibody responses in vivo.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Qi, Hai -- Egen, Jackson G -- Huang, Alex Y C -- Germain, Ronald N -- Intramural NIH HHS/ -- New York, N.Y. -- Science. 2006 Jun 16;312(5780):1672-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Lymphocyte Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Bethesda, MD 20892, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16778060" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antigens/*immunology
;
B-Lymphocytes/cytology/*immunology
;
Calcium/metabolism
;
Calcium Signaling
;
Cell Movement
;
Cell Shape
;
Coculture Techniques
;
Dendritic Cells/*immunology
;
Endocytosis
;
Lymph Nodes/cytology/*immunology
;
*Lymphocyte Activation
;
Mice
;
Mice, Inbred C57BL
;
Mice, Transgenic
;
Muramidase/immunology
;
Receptors, Antigen, B-Cell/immunology
;
T-Lymphocytes/immunology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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