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  • starch composition  (5)
  • plant regeneration  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Revertants of the amylose-free potato clone 86.040 (2n = 1x = 12)
    Amsterdam : Elsevier
    Plant Science 67 (1990), S. 177-182 
    Details
    ISSN: 0168-9452
    Keywords: granule bound starch synthase ; mutation ; potato ; reversion ; starch composition
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(90)90241-F
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  • 2
    Electronic Resource
    Electronic Resource
    Rapid estimation of the amylose/amylopectin ratio in small amounts of tuber and leaf tissue of the potato (1988)
    Springer
    Potato research 31 (1988), S. 241-246 
    Details
    ISSN: 1871-4528
    Keywords: minitubers ; starch composition ; mutant detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Small amounts of potato tuber and leaf tissues are extracted in perchloric acid. After staining with I2-KI solution absorbancies at 618 and 550 nm are measured. The amylose/amylopectin ratio can be estimated from the ratio of the absorbancies by using a formula or a graph in which the specific absorptions of the two compounds are introduced. Weighing of samples is not required. The method can be used for the estimation of the starch composition of minitubers and is especially suitable for the detection of mutants with an altered starch composition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02365532
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  • 3
    Electronic Resource
    Electronic Resource
    Induction, germination and shoot development of somatic embryos in cassava (1993)
    Springer
    Plant cell, tissue and organ culture 33 (1993), S. 151-156 
    Details
    ISSN: 1573-5044
    Keywords: cassava ; plant regeneration ; somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four Indonesian and two Latin-American cassava genotypes (Manihot esculenta Crantz), were evaluated for their ability to develop somatic embryos from young leaf lobes. All genotypes formed somatic embryos but they differed in the frequency of embryos induced. The best genotypes, M. Col 22 and Tjurug, produced germinating embryos (GE) on 81% (22.1 GE/initial leaf lobe) and 46% (4.3 GE/initial leaf lobe) of the cultured leaf lobes, respectively. Up to 57% of the germinating embryos of M. Col 22 and 12% of Tjurug produced either normal or malformed shoots. Most malformed shoots developed into shoots with normal morphology after prolonged culture. All shoots formed roots after transfer to medium without BAP. Roots of all normal and most malformed regenerants had the original ploidy level (2n=36). Regardless of whether the plants were multipliedin vitro (150 plants) or in the greenhouse (30 plants) there were no morphological differences compared to parent plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01983228
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  • 4
    Electronic Resource
    Electronic Resource
    Field evaluation of antisense RNA mediated inhibition of GBSS gene expression in potato (1991)
    Springer
    Euphytica 59 (1991), S. 83-91 
    Details
    ISSN: 1573-5060
    Keywords: Agrobacterium rhizogenes ; antisense RNA ; granule-bound starch synthase ; Solanum tuberosum ; starch composition ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Granule-bound starch synthase (GBSS) catalyses the synthesis of amylose in starch granules. Analysis of antisense RNA mediated inhibition of GBSS gene expression in large numbers of tubers from in vitro grown, greenhouse grown and field grown transgenic potato plants revealed stable and total inhibition of GBSS gene expression in one clone. In three other transgenic genotypes partial and unstable inhibition was found. In these genotypes both GBSS activity and amylose content were remarkably reduced compared with the non-transformed control genotype. No relationship was found between the level of inhibition of GBSS gene expression and yield and dry matter content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025364
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  • 5
    Electronic Resource
    Electronic Resource
    Increased frost tolerance and amino acid content in leaves, tubers and leaf callus of regenerated hydroxyproline resistant potato clones (1987)
    Springer
    Euphytica 36 (1987), S. 369-380 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; amino acids ; amino acid analogue resistance ; frost tolerance ; plant regeneration ; proline ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A number of previously selected hydroxyproline (hyp) resistant cell lines of a diploid potato (Solanum tuberosum L., clone H2578, 2n=2x=24) could be regenerated into plants which were further analysed. Hyp resistance, although lower than in the originally selected calli, was still present in regenerated shoots and in callus initiated from these shoots and it was not lost upon (mini)tuber propagation. Regenerated shoots showed a wide range of phenotypic variation. The chromosome number, analysed in 4 clones, appeared to be hypotetraploid (44 or 45). Tuber-propagated regenerants generally showed increased frost tolerance both at the plant and the cell level. In leaves this seemed to be associated with increased levels of both proline and total amino acid content. However, in callus only the total amino acid content but not proline was still elevated. In the wild type the frost tolerance of the tubers appeared to be lower than that of the leaves. Between tubers of wild type and the hyp resistant regenerants no differences in frost tolerance were found, although proline and total amino acid content tended to be higher in tubers from the regenerants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00041480
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  • 6
    Electronic Resource
    Electronic Resource
    Phenotypic and genotypic characterization of an amylose-free starch mutant of the potato (1989)
    Springer
    Euphytica 44 (1989), S. 43-48 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; mutant ; starch composition ; amylose-free ; embryo rescue ; inheritance ; phenotype ; breeding ; karyotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The amylose-free (amf) potato mutant 86.040 has been characterized phenotypically and genotypically. Not only storage starch in tubers and metabolic starch in leaves but also starch in cells with specific functions, such as columella cells in the root cap and guard cells of stomata, was amylose-free. Doubled amf-clones of 86.040 flowered well, but the percentage of stainable pollen was low (8.2%). Therefore, they were used as female parent in crosses with diploid amylose-containing plants. Over 400 pollinations gave 13 berries, all dropping off prematurely; they contained 78 ovules with embryos at different developmental stages. Twenty F1 plants were obtained by embryo rescue, all having amylose starch. Three of them were characterized more extensively. They flowered well and pollen stainabilitity as well as seed set were high. Analysis of the F1 plants and of F1 × F1 progeny plants showed that the amf-character is monogenic and recessive. The locus involved was designated amf +and the mutated allele amf.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022597
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  • 7
    Electronic Resource
    Electronic Resource
    Cytological studies on adventitious shoots and minitubers of a monoploid potato clone (1988)
    Springer
    Euphytica 39 (1988), S. 213-219 
    Details
    ISSN: 1573-5060
    Keywords: Solanum tuberosum ; potato ; adventitious shoot induction ; minituber induction ; starch composition ; cytology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A three step procedure for adventitious shoot regeneration on leaf explants of monoploid potato clone H7322 and a minituber induction procedure on stem segments have been described. Chromosome counts on 92 adventitious shoots showed that 85% of them had been polyploidized, i.e., 71% were diploid, 1% tetraploid, and 13% were mixoploid. Cytophotometric studies on nuclei of soil grown tubers of tetraploid cv Astarte, of 1x, 2x and 4x adventitious shoots of H7322, and of diploid H2578 showed in all cases polyploidization with prominent classes up to 8C and 16C. However, nuclei of pith cells of 5 weeks old minitubers which had developed on monoploid H7322 itself or on 1x adventitious shoots of H7322 showed predominantly 1C and 2C values. Pith cells of minitubers of monoploid H7322 were screened, after iodine staining, for the presence of variant cells containing reddish-brown staining (amylose-free) starch. In more than 75% of the investigated minitubers one or a few of such variant cells were found indicating that such a variation occurs in minitubers of monoploid potato and that this variant character is expressed in cells of vegetative storage organs like potato tubers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037098
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  • 8
    Electronic Resource
    Electronic Resource
    Secondary somatic embryogenesis and applications in plant breeding (1995)
    Springer
    Euphytica 81 (1995), S. 93-107 
    Details
    ISSN: 1573-5060
    Keywords: plant regeneration ; cyclic somatic embryogenesis ; plant breeding ; review
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Secondary somatic embryogenesis is the phenomenon whereby new somatic embryos are initiated from somatic embryos. Such cultures have been described in at least 80 Gymnosperm and Angiosperm species. In the initial step (primary somatic embryogenesis) such cultures have to be started from plant explants. In general, primary somatic embryogenesis from vegetative plant explants is, indirect and mostly driven by auxin (AUX) or auxin and cytokinin (AUX/CYT) supplemented media, whereas, from zygotic embryos it is direct and driven, to a larger extent, by CYT or growth regulator free media. Primary somatic embryogenesis from floral plant explants is between these two extremes. Indirect and direct somatic embryogenesis should be seen as two extremes of one continuum: in indirect somatic embryogenesis the embryos develop up to the (pre)-globular stage and in direct somatic embryogenesis to mature stages before they are subjected to secondary embryogenesis. In general, secondary embryogenesis requires no growth regulators in species with CYT driven primary embryogenesis. Whereas, continuous exposure to growth regulators is needed in species with CYT/AUX or AUX driven primary embryogenesis. In most species somatic embryos can be converted into shoots, although the frequencies are mostly low. In general, somatic embryos induced by growth regulator free or CYT supplemented media meet more difficulties in shoot development than embryos induced by AUX supplemented media. Applications of secondary somatic embryogenesis for plant breeding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022463
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