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  • Litopenaeus vannamei  (24)
  • Health  (8)
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  • 1
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    Development gene data bank of cultured shrimp species in Iran (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. in this research, samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: DNA barcode ; Shrimp ; Litopenaeus vannamei ; Penaeus semisulcatus ; Fenneropenaeus merguiensis ; Metapenaeus affinis ; Parapenaeopsis stylifera ; Fenneropenaeus indicus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 72pp.
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  • 2
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    Probiotic effects of Bacillus spp. bacteria on the survival and growth of White shrimp (Litopenaeus vannamei) in 30 and 40 g/lit salinities (2018)
    Details
    Publication Date: 2021-05-19
    Description: The probiotic effects of Bacillus spp. bacteria on the growth and survival rate of Litopenaeus vannamei in 30 and 40 gii, salinities was investigated. We conducted the experiment in Bandargah Research Station, Bushehr province during 45 days. Four treatments with three replicates, and two controls were used and probiotic powder, dissolved in water along with the growth stage pellet was applied in 3Oppt and 4Oppt salinities. The control treatments were only fed by the growth stage pellet in 3Oppt and 4Oppt salinities. The results revealed that growth and survival rate of treatment groups in 3Oppt salinity exposed to the probiotic powder were higher than those of the controls without increasing probiotic powder (P〈0.05). Although the growth rate of treatment groups in 4Oppt salinity was higher than those of the control without increasing probiotic powder in 4Oppt salinity, but there was no significant difference between the two (P〉0.05). Survival rate of the treatment groups in 4Oppt salinity was significantly higher than those of the control (P〈0.05). Also, the results indicated that growth and survival factors of the treatment groups in 3Oppt salinity in comparison with those of the control in 4Oppt salinity were significantly higher. We conclude that addition of Bacillus spp. bacteria to 3Oppt salinity together with application of growth stage pellets to white shrimp, Litopenaeus vannaiiiei, could be very useful in comparison with 4Oppt salinity. The results of this study were obtained through ANOVA and LSD tests.
    Description: Published
    Keywords: Shrimp culture ; Litopenaeus vannamei ; Bacillus ; Growth rate ; Salinity effects ; Survival ; Marine
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.27-34
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  • 3
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    The effects of density on culture of Litopenaeus vannamei in laboratory condition (fiberglass tank) (2018)
    Details
    Publication Date: 2021-05-19
    Description: The white leg shrimp (Litopenaeus vannamei) was introduced to Iran in 2005 by Iranian Fisheries Research Organization. In this study which was carried out at the Iran Shrimp Research Center in Bushehr, shrimp growth in three density treatments 50, 100 and 150 PCs/m super(3) with three replications were compared. The experiment was conducted for 50 days in fiberglass tanks (three ton capacity). Daily tests of salinity, pH ,temperature and dissolved oxygen were also carried out. We found that the mean salinity, pH, temperature and dissolved oxygen were 30.2 ppt, 7.9, 25.5 degrees centigrade and 6.2mg/l respectiely. We recorded a mean weight of 2.96, 2.17 and 1.87 grams for shrimps in 50, 100 and 150 PCs/m super(3) respectively.
    Description: Published
    Keywords: Stocking (organisms) ; Shrimp culture ; Culture tanks ; Fibre glass ; Litopenaeus vannamei ; Juveniles ; Tolerance ; Growth ; Density ; Survival ; Marine
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.159-164
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  • 4
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    Evalution close relationship between broodstock and seeds of Litopenaus vannamei each generation (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: In this study, two population of Molokaei and High health were selected as zero generation of Litopenaeus vannamei broodstocks. After mating males and females adult within inbreeding and crossbreeding three various storage were produced: H♂ × M♀, M♂ × H♀ and H♀ × H♂. The second generation of shrimp were obtained from storage of broodstocks (MH) first generation. The aim of this study was investigated close relationship between different generations of specific pathogenic free shrimp. The analysis d-loop region of mitochondrial genomes of different generations shrimp was observed 997 positions with 3-6 and 766-799 of haplotype and monomorphic, respectively. Despite haplotype diversity and nucleotide diversity of all generations SPF shrimp were 0.877 and 0.12, respectively. But, results showed homozygosity rate is increasing from one generation to next generation, so low genetic distance were observed between different generations of SPF shrimp certain disease-free shrimp. Regarding the results of in this study can be concluded that lack of arriving new broodstocks from outside the country and was mating between adult males and females relative (full-sib) addition to small effective population size there was both genetic drift and number of haplotypes was gradually reduced from zero generation to second generation. Hence, due to the high close relationship between different generations shrimp evaluation of d-loop region of the mitochondrial genome were little genetic difference between them
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; Mitochondrial genome ; D-loop region ; Close relationship ; Population ; Males ; Females ; Adult
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 54pp.
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  • 5
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    Supply and protected different population of Litopenaus vannamei subadult zero foster (F0) from difference provices Iran (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Nowadays, Litopenaeus vannamei are the most important species of farmed penaeidae shrimp in the world that is rapidly replacing native species in areas aquaculture. Due to demand increase for this species culture, shrimp displacement to different areas may be associated with some potential pathogens transferred to new areas farmed. Therefore, in this study were prepared bi-osecurity conditions for specific disease-free production of L. vannamei. Thereafter, three populations (Molokaei, High health and mix of Molokaei and High health) of the shrimp various reserves were detected base on origin and genetic indexes such as: observed heterozygosity, expected heterozygosity, allele frequency, coefficient inbreeding, genetic differentiation, genetic distance and genetic identity. On the other hand, epidemiological studies indicate non pathogens (viral, bacterial, fungal and parasitic) recognition of different populations selected in the quarantine salon. The bioassay results showed that the average weight and length of the populations of High health and Mix significantly greater than was a population of Molokaei. The shrimp populations were stocked in fiberglass tank (five ton) and were kept separated in the quarantine salon. During maintenance shrimp of populations in the quarantine salon were evaluated living and non-living pathogens with PCR, microbiology and biochemical methods. There is not any pathogens detection from shrimp populations stocking in the quarantine salon, so the shrimps were carried over to pond for broodstock culture of specific pathogenic free.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; Specific pathogenic free ; Bi-osecurity ; Population
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 48pp.
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  • 6
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    Monitoring, Isolation and identification bacterial and parasitic agents in specific pathogen free shrimp production (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Aquaculture is the fastest growing food industry in the world. Shrimp culture industry is also part of it, unfortunately, like other marine animal culture economic losses caused by the disease has been one of the major challenges of this industry. The major cause of mortality in shrimp hatcheries and rearing centers is related to water quality and the presence of pathogenic bacteria and parasites. These are common opportunistic microorganisms in the hatchery, rearing centers, flora and living food but poor conditions of culture are caused diseases. Since the development of aquaculture in the countries need health management, one of the important additional rings in the shrimp strategic plan is specific pathogen free shrimp production, which has been addressed in this plan. Specific pathogen free shrimp define as the shrimps which are free of the specific pathogens listed in world organization for animal health (OIE). These factors should be conclusively diagnosed and can be isolated from shrimp hatcheries and culture system. Therefore in this project screening and surveillance of shrimp in several generations according to the list of OIE were done and they were monitored for of bacterial pathogens (Necrosis Hepatopancreas Bacteria) and parasites (Microsporidian and Gregarins). At total 756 pieces of shrimp, 6 sample of dry food and 97 samples of live foods were controlled and tested. 1.35 percent of live foods were positive for NHPB and 5.6 percent of pre broodstocks have eppicommensal and microsporidia which were disposed in quarantine phase. Because of biosecurity and surveillance system establishment, there were no bacterial or parasitic isolation or diagnosis during SPF shrimp production.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; SPF ; Bacteria ; Parasite ; Aquaculture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 60pp.
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  • 7
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    Study on effect of enriched Artemia with (HUFA) on reproductive parameters and comparing with current method (used marine worm) in Litopenaeus vannamei (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: Nowadays, the use of live food enrichment is very common in aquaculture and as a practical method introduced. Artemia a potential food source and quality is suitable, it will have used for feeding fish and crustaceans (shrimp). In this study, Artemia enriched with unsaturated fatty acids used for increase the sexual maturity of the white leg shrimp (Litopenaeus vannamei). Thus, the young brood stocks transferred to Bandarga Research station. In order to achieve optimal weight, Brood stocks were feeding by food concentrate and food frozen fresh. In this study such as 3 treatments each with 3 replicates, which brood stocks were stocking in the fiberglass thank with density 2 to 3 (pieces/m2). They were feeding with meals different including 1) squid , pen shale and marine polychaeta worm (Perinereis nuntia), 2) squid , pen shale and Artemia biomass "fransiscan", 3) squid , pen shale and Artemia biomass enriched with unsaturated fatty acid (HUFA) SPARI SELCO solution after were eye ablation. The results showed that sexual maturity increased in the brood stocks fed with Artemia enriched which with significantly higher brood stocks fed with Artemia biomass and the marine worm (P 〈0.05) whereas significant difference was not found in the rate of sexual maturity brood stocks fed with Artemia biomass and marine worm (P〉 0.05). The other hand, histological studies showed that brood stocks fed with Artemia enriched than other two treatments contain were many mature follicles ready for ovulation. However, there was more absolute fecundity in the brood stocks fed with Artemia enriched, but don't found any significant differences in competition with other two treatments (P 〉 0.05). In addition, during this period, measured and recorded all factors physicochemical water and environment daily. Following this study showed that if the Artemia enriched as part of the diet can both caused eliminate unsaturated fatty acid deficiency diet and improved ovarian development and increase sexual maturity brood stocks female in a short interval. Finally, the results analyzed with statistical software spss 9.0, one way ANOVA and Tukey test.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Histological ; Physicochemical ; White leg shrimp ; Litopenaeus vannamei ; Artemia ; Biomass ; Enrichment ; Sexual maturation ; HUFA ; Aquaculture ; Feeding ; Fatty acids ; Brood stocks ; Density ; Squid ; Polychaeta worm ; Perinereis nuntia ; ANOVA
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 45pp.
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  • 8
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    Study of growth, survival rate and final harvesting of Litopenaeus vannamei impact of 25, 35 and 50 (per/m2) stocking densities (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publication Date: 2021-05-19
    Description: For the first time in research program and species diversity the white legged shrimp (Litopenaeus vannamei) was introduced in Iran in 2005 by Iranian Fisheries Research Organization. In two recent years two projects was carried out successfully in Hele Research Station and according of these projects study of growth, survival rate and final harvesting of Litopenaeus vannamei impact of 25, 35 and 50 (per/m2) stocking densities was done .Nine 0.4 ha earthen ponds were stocked from 26 June 2006 to 11 November 2006 and the rate of stocking was 100000,140000 and 200000 Pl in 25, 35 and 50 per /m2 respectively. We also carried out daily test of pH, temperature and dissolve oxygen two times per day but transparency, depth and salinity one time per day. The mean weight in 120 days was 17/84±2/6, 16/03±1/9 and14/09±2/3 grams in 25, 35 and 50 per /m2 respectively and highest weight was seen in 25 density. Results demonstrated that there was a significant relation (P〈0.05) between mean weight. The yield were 1745, 2055 and 2425 kg/pond although 4/3, 5/1 and 6 ton/ha in 25, 35 and 50 per /m3 respectively. The maximum and minimum survival rate 97/96 and 88/46 was seen in 25 and 50 per/m2. The minimum FCR 1.43 was seen in 25 per /m2. For economical calculating just by using the cost of larva and feeding indicates that the highest benefit percentage was seen in 25 density. The average of salinity, pH, temperature and dissolve oxygen were 36.1-37 ppt, 8.1, 30.2-30.6 degrees centigrade and 5.9- 6.4 mg/l.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; Density ; Mean Weight ; Species ; Diversity ; Shrimp ; Growth ; Survival rate ; pH ; Temperature ; Dissolved oxygen ; Transparency ; Salinity
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 29pp.
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  • 9
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    (Fenneropenaeus indicus) study on protozoa emerging diseases of shrimp (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25494 | 18721 | 2018-10-05 07:36:19 | 25494 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Certain diseases such as bacterial , viruses, parasirtic ones Protozoa parasites Could severe mortalities in farmed shrimp last decades. Two hundred and seventy Fenneropenaeus indicus with the weight of 10± 1.09 were selected, transferred to Iran Shrimp Center and distributed to the treatmentsand controls (3 groups). The objective of our study was to describe the signs, prevalence, and intensity of Hemocytosis, a new disease in shrimp, Fenneropenaeus indicus. The experimental design included three groups, one treatment as infected shrimp or group B and 2 controls with non-infected but exposure to White Spot Syndrom Virus (WSSV) shrimp as group A and non-infected non- exposure to WSSV shrimp as group C in triplicate. The results revealed that Hemocytosis associated with a decrease in hyalinocytes and large-granulocytes (less than 8%) and a considerable increase in semi-granulocytes in group A and B in contrast to group C that Hyalinocyte increased. This infection made the shrimp susceptible to WSSV disease. It is believed that Hemocytosis causes a condition which can result in a rapid mortality among susceptible species, F. indicus when exposure to WSSV is occurred.
    Keywords: Health ; Iran ; White Spot Syndrome Virus ; Fenneropenaeus vannemai ; Challenge ; Protozoa ; Shrimp ; Fenneropenaeus indicus ; Bacterial ; Parasites ; Mortalities ; Hemocytosis ; Hyalinocyte ; Species ; F.indicus
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 56
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  • 10
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    Implementation biosecurity standard in shrimp specific pathogen free (SPF) research pilot study (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25552 | 18721 | 2018-10-05 17:30:01 | 25552 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-16
    Description: Biosecurity means” all activities that decrease the introducing and expanding the pathogen in the shrimp farm” the main activity consisit physical, chemical and biological methods. Some factores has low risck and some of them have high risck level. The level of biosecurity in the shrimp farm and hatcheries is depends the equipmemt and expense that used in the program. For each situation in biosecurity, the scientist will be prepared the standard operation program (SOP) for each risck factor.In the Specific Pathogen Free program that conduted in Bousher, 10 SOP design for different risck factor in the banderga station and Persian Golf Centre for SPF production. The SOP, detecting the main pathogen in shrimp farm in shrimp, feed, water and any equipment in the project. The SOP also consider the disinfectant water with physical and chemival methods such as different net in different size, chemical such as chlorine and ozon and biological filter. During the project studied the methods exclude all pathogen from shrimp and feed and decreased the risck factors in program. The result showed if the culturist used these methods in the production, the safty of shrimp production increased and the sustainable shrimp culture will be available.
    Keywords: Health ; Iran ; Biosecurity ; Ciritical point ; Standard Operation Porogram ; Disaese ; SOP ; Implementation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 65
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