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  • Fisheries  (11)
  • Genetic  (3)
  • 1
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    Comparison of genetic variation of Ship sturgeon (Acipenser nudiventris) in the southern Caspian Sea and Ural River using PCR-RFLP (2018)
    Details
    Publication Date: 2021-05-19
    Description: Genetic variation of ship sturgeon (Acipenser nudiventris) from the Caspian Sea was investigated using NADH5/6 gene and PCR-RFLP analysis. A total of 80 specimens of the fish were collected from the south Caspian Sea and the Ural River from Kazakhstan. mtDNA ND5/6 gene was amplified by polymerase chain reaction (PCR) digested using 39 Endonucleases Restriction Enzyme. Of the 39 enzymes, five showed polymorphism. Totally, ten composite haplotypes among 80 specimens were detected. Haplotype AAAAA showed maximum frequency (57.5%) whereas haplotypes BBAAA and BABAA showed minimum frequency (1.2%). Haplotype AAAAB was recognized specifically in Ural River specimens. Average haplotype and nucleotide diversity was 0.8516 and 0.007 respectively. Compared to other sturgeon species living in the Caspian Sea, nucleotide diversity of Ship Sturgeon was much lower (0.007). This may be due to smaller population size of this species. Monte-Carol simulation using 1000 interaction did not show any significant differences between haplotype distribution of the fish sampled in the south Caspian Sea (X2=35.48 , P=0.74). However, we detected a significant difference between haplotype of Ship Sturgeon from Ural River and the south Caspian Sea. We conclude that Ship Sturgeon from Ural River is different from the fish in the south Caspian Sea and suggest Cfr131 enzyme as a molecular marker for population differentiation in the Caspian Sea.
    Description: Published
    Keywords: Ship sturgeon ; Acipenser nudiventris ; Ural River ; PCR-RFLP ; ND5/6 gene ; Enzyme ; Diversity ; Population ; Species ; Genetic ; Specimens
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.151-164
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  • 2
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    Effects of lecithin on growth and hematological indices in juveniles of Siberian sturgeon (Acipenser baeri Brandet 1869) (2021)
    In:  http://aquaticcommons.org/id/eprint/21857 | 18721 | 2018-01-12 14:43:59 | 21857 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-03
    Description: This study was carried out to evaluate the effects of dietary lecithin on growth performance and hematological indices in juveniles of Siberian sturgeon (Acipenser baeri). Fish with initial average weight of 32.9±0.3 grams were fed five isoproteic and isolipidic formulated diets with different soybean lecithin levels including 0, 2.5, 5, 7.5 and 10% in triplicate groups for 8 weeks. Results showed that lecithin supplementation to 7.5% significantly increased some growth indices such as body weight increase (BWI), specific growth rate (SGR), feed conversion ratio (FCR ) final weight (Wf), condition factor (CF) and protein efficiency ratio (PER). Also, increasing dietary lecithin to the level of 10% significantly decreased growth indices. According to the results of hematological assays, hemoglobin (Hb) in treatments of 5% and 7.5% in comparison to other treatments had higher value. Hematocrit (Hct) in fish fed with 7.5% lecithin was higher than those fed with 0 and 2.5% lecithin in the diets. Other hematological indices such as WBC, RBC, MCV, MCH and MCHC showed no significant differences among the treatments. In conclusion, the results of this study indicated that Siberian sturgeon juveniles have a relatively moderate lecithin requirement (between 5 and 7.5 percent of the diet) and these levels of lecithin in diet induces increase of growth performance and improves health status of this species.
    Keywords: Aquaculture ; Biology ; Fisheries ; Diet ; Food items ; Siberian sturgeon ; Growth ; Hematology ; Acipenser baeri ; juvenile ; iran ; WBC ; RBC ; MCV ; MCH ; MCHC
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 143-154
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  • 3
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    Effects of incubation and larval rearing temperature on histological changes of muscles in Caspian trout (Salmo trutta caspius Kessler, 1877) larvae (2021)
    In:  http://aquaticcommons.org/id/eprint/22101 | 18721 | 2018-02-08 03:06:51 | 22101 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-06-26
    Description: Out of considerable environmental factors, temperature and its possible effects on life stages of Caspian trout investigated by natural incubation (8 ͦC) condition simulation comparing with cold (4 ͦC) and warm (12 ͦC) constant incubation temperatures in 3 well equipped incubators by water recycling systems. Green eggs triple treatments of wild and F1 cultured brooders were incubated. Incubation implemented in dark by using REDD water and DO–pH – temperature digital monitoring ended to yolk sac absorption and entering larval stage. Numbers and diameters of white fiber muscles measured and significant differences considered between three thermal treatments (P〈0.05) in both wild and cultured stocks. The numbers of white fiber muscles in warm treatment by highest means (72.54) and lowest diameter (8.46 micrometer) compared with Cold treatment white fiber muscles diameter (20.59 micrometer) and numbers (50.72) which were the highest diameter and lowest numbers means between treatments. Hatching success stated considerable mortality for cold treatments and 8°C incubator improved the best temperature in wild treatment. Incubation temperature induced significant effect on white fiber muscles stated considerable index for flesh precursor muscles condition which is subject of natural stocks rehabilitation and domestication projects considerations.
    Keywords: Biology ; Fisheries ; Caspian trout ; Temperature ; White fiber muscle ; Domestication ; Stocks rehabilitation ; Salmo trutta caspius ; larvae ; temperature ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 95-104
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  • 4
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    Optimization of UV irradiation for production of gynogenetic rainbow trout, Oncorhynchus mykiss: emphasising Hertwig effect and photoreactivation (2021)
    In:  http://aquaticcommons.org/id/eprint/22287 | 18721 | 2018-03-13 02:18:42 | 22287 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-02
    Description: The optimum dose of UV irradiation to produce gynogenesis rainbow trout, Oncorhynchus mykiss with emphasis of Hertwig effect and photoreactivation (PR) was investigated. For this purpose, the sperm of rainbow trout were irradiated with UV at 2010±200 µw.cm-2intensity in different alternatives of 0, 1, 3, 5, 8, 10, 15, 20, 25, 35 and 45 minutes and allowed to fertilize normal ova; the fertilization, eyed and hatching rate were calculated to assess the performance. Using the irradiated sperm decreased the fertilization, eyed as well as hatching rates and the so-called "Hertwig effect" was observed, with the time-dependent decrease in hatching rate at 0+to 10 min of irradiation, but a better hatching rate at greater time of irradiation was observed. The highest hatching rate was observed in 20 min of UV irradiation; after that the survival rate decreased rapidly. Interestingly, irradiation even up to 45 min could not eradicate sperm fertility and a hatching rate above zero was observed at this treatment. For PR studies the semen was irradiated with UV for 5, 30 and 120 seconds and untreated semen (Os) was used as control. Irradiated semen and/or fertilized eggs by treated semen were exposed to visible light (60 W) at a distance of 30 cm for 10 min; the eyed and hatching rates were measured. The results showed that UV irradiation in as low as 5s could cause deleterious effect on semen chromatin and decrease the eyed and hatching rates (p〈0.05). Illumination of semen and\or eggs with visible light resulted in PR in rainbow trout when sperm was irradiated by UV at 120s. We could conclude that the best UV irradiation time for production of gynogenesis rainbow trout was 20 min and UV irradiation, fertilization and egg hardening must be done far from visible light to eliminate the PR mechanism.
    Keywords: Biology ; Fisheries ; Gynogenesis ; Hertwig effect ; Oncorhynchus mykiss ; Photoreactivation ; Rainbow trout ; Ultraviolet ; Iran irraciation
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 19-34
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  • 5
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    Inheritance of RAPD marker in female grass carp (Ctenopharyngodon idella), male bighead carp (Hypophthalmichthys nobilis) and their F1 hybrids (2021)
    In:  http://aquaticcommons.org/id/eprint/22333 | 18721 | 2018-03-21 22:25:24 | 22333 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-03
    Description: Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic DNA. Results from 77 produced bands showed that all RAPD bands pattern of parents were present in F1 offspring hybrid, which indicated the high influence and dominance of the mentioned markers. The low difference in polymorphisms in used markers between F1 offspring hybrid and parents shows that parents are heterozygous in some loci, which can cause low difference in hybrids of two distinct genera. Although our results revealed that RAPD markers had a suitable efficiency in distinguishing parents from F1 hybrids, genetic diversity of triploid and diploid F1 hybrid were not detected by introduced primers.
    Keywords: Biology ; Fisheries ; RAPD Marker ; Grass carp ; Bighead carp ; Hybridization ; Triploidy ; Hypophthalmichthys nobilis ; Ctenopharyngodon idella ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 47-56
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  • 6
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    Microsatellite polymorphism reveals low genetic differentiation between fall and spring migratory forms of endangered Caspian trout, Salmo trutta caspius (Kessler, 1870) (2021)
    In:  http://aquaticcommons.org/id/eprint/21692 | 17408 | 2017-11-27 11:25:55 | 21692 | University of Guilan, Faculty of Natural Resources, Iran
    Details
    Publication Date: 2021-06-28
    Description: The main objective of this study was to assess genetic comparison of two migratory forms of Caspian trout Salmo trutta caspius namely fall-run and spring-run. Owing to the lack of information on its genetic differences, 5 microsatellite loci were used for 58 sample analyses. Genomic DNA was extracted from caudal fin using Roche DNA extraction kit and each PCR reaction was performed in a 25 µl reaction volume. Results revealed that the most allelic frequencies were observed in fall-runs of Caspian trout. The average observed and expected heterozygosity in fall-runs and spring-runs were 0.7719, 0.6108 and 0.4435, 0.5911, respectively. In both groups except Str543INRA in spring runs, all loci had deviation from Hardy Weinberg equilibrium. Furthermore except Str543INRA in spring runs, expected heterozygosity in all loci was more than observed heterozygosity. In conclusion microsatellite loci polymorphism in this study reveals low genetic differentiation between fall-runs and spring-runs. In order to increase gene flow between Caspian trout populations of different rivers and to increase the production of these fishes, restoration of rivers habitats, as well as using more breeders originating from various rivers is highly recommended.
    Keywords: Biology ; Fisheries ; Salmo trutta caspius
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 9-16
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  • 7
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    Analysis of saline activator solution effects on sperm quality indices of Barbus sharpeyi by Image J software (2021)
    In:  http://aquaticcommons.org/id/eprint/22604 | 18721 | 2018-05-01 23:37:40 | 22604 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-08
    Description: Regarding low fertilization rate of Barbus sharpeyi, one of the main Barbus species in south of Iran, saline activator solutions (SAS 1 and 2) were used for increasing of sperm activation and its sperm quality indices were assessed by using Image J software as a fish sperm adapted tools. SAS1 (45mM NaCl, 5mM KCl, 30mM Tris, 188±2.3 mOsmol/kg) and SAS2 (50mM NaCl, 30mM KCl, 30mM Tris, 189±1.7 mOsmol.kg) were added to a mixture of 12 male semen comparing with distilled and tap water and their effects on VCL, VSL, ALH, Linearity, %motility, spermatozoa path, % A, B and C spermatozoa type, fertilization, hatching and deformity rate were analyzed in triplicate. Results showed that SAS2 had the highest VCL (21.37±0.36 µm/s), VSL (11.6±0.3 µm/s) and motility percentage (85.75±1.56 %) among treatments. The highest A type spermatozoa percentage was recorded for SAS1 (47.16±3.15 %) and SAS2 (43.89±4.54%). The lowest C type spermatozoa percentage was recorded using SAS2 (14.24±1.56 %). Also, the highest fertilization rate was achieved from SAS2 (79.5±0.37%) and SAS1 (77.74±4%) respectively. Furthermore the lowest larval deformity rate (13.77±1.03%) was shown due to the use of SAS2. Final conclusion confirmed that SAS1 and SAS2 have positive and significant effects (P 〈 0.05) on extending the time length of sperms movement, VCL and VSL of B. sharpeyi which consequently resulted in better efficiency of propagation of this species in south of Iran.
    Keywords: Biology ; Fisheries ; Sperm ; Saline activator solution ; Fertilization ; Barbus sharpeyi ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 357-377
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  • 8
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    Karyological study on bighead goby (Neogobius kessleri) from southern part of the Caspian Sea (2021)
    In:  http://aquaticcommons.org/id/eprint/22312 | 18721 | 2018-03-20 17:55:05 | 22312 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-03
    Description: Karyological characteristics of bighead goby (Neogobius kessleri) in the Caspian Sea were studied by examining 30 metaphase chromosome spreads from the kidney tissue of 10 specimens. The chromosome number of this species was found 2n=46 and the arm number as NF=46. The prepared karyotype of this species consisted of 23 pairs acro-telocentric (a-t) chromosomes. The chromosomal formula can be stated as 2n=46 (a-t). Karyological parameters showed that relative length was between 2.34-7.04 and length variation range of chromosomes was between 1.67-5.01 and total length was 71.16µm. It was found that the best chromosomal spread quality were obtained from intraperitonial injection of 40µg/g colchicine for 5 hours, hypotonization of samples in %1 sodium tri-citrate solution in 4ºC and preparation of spreads on cooled slide with flame technique.
    Keywords: Biology ; Fisheries ; Chromosome ; Karyology ; Bighead goby ; Neogobius kessleri ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 15-26
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  • 9
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    Mixed milt fertilization of endangered Caspian brown trout Salmo trutta caspius influences effective population size of breeders (2021)
    In:  http://aquaticcommons.org/id/eprint/22784 | 18721 | 2018-05-22 17:50:27 | 22784 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-10
    Description: The maintenance of the endangered Caspian brown trout Salmo trutta caspius populations in Iran depends on its artificial breeding. There is no information on how current hatchery breeding protocol influences effective population size of breeders (Ne), which is a critical parameter to maintain genetic diversity in future generations. The current hatchery protocol (CHP) was comparatively evaluated with a balanced hatchery protocol (BHP), where mixtures of equal amounts of gametes per male and female breeder were used to balance parental contributions to progeny. To evaluate putative differences in viability between families, individual crosses were performed and fertilized ova of different families were mixed to constitute balanced family pools. 1440 alevins were totally sampled in the crosses performed from the 11 breeders. An exclusion-based parentage approach using three polymorphic microsatellite markers unambiguously assigned more than 93% of progeny to a single pair of parents. Significantly different contributions of breeders to progeny were observed in CHP (p〈0.05). The primary constraint on Ne in BHP was the unbalanced contribution of males, which seemed a consequence of sperm competition in mixed fertilization caused by differences in sperm quality. Sperm motility duration was positively correlated with the number of sired progeny by each male. The results illustrate the limitations of the BHP in minimizing the loss of genetic diversity observed in CHP. A protocol based on mixture of equal number of fertilized ova from individual male × female crosses emerged as the best alternative for conservation of Caspian brown trout.
    Keywords: Aquaculture ; Biology ; Conservation ; Fisheries ; Caspian brown trout ; Salmo trutta caspius ; Mixed milt fertilization ; Effective population size ; Sperm competition ; Microsatellite ; aquaculture ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 393-408
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  • 10
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    Conspecific relation between two seasonal migratory forms of endangered Caspian trout, Salmo trutta caspius Kessler, 1877, revealed by RAPD markers (2021)
    In:  http://aquaticcommons.org/id/eprint/22435 | 18721 | 2018-04-09 17:55:33 | 22435 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-05
    Description: Random amplified polymorphic DNA (RAPD) markers were used to estimate genetic relationships between two seasonal immigrant forms (namely fall-run and spring-run) of Caspian trout Salmo trutta caspius. In this regard, 62 fin tissue samples were collected from southern parts of the Caspian Sea basin and examined with 16 oligodecamer primers to asses their genetic diversity as well as probable specific population bands. The total number of RAPD bands produced in spring and fall-run were 162 bands, of which 69 and 53 bands were polymorphic for each population. Nei's genetic identity and genetic distance between spring-run and fall-run populations were 0.9858 and 0.01430, respectively. The RAPD based data revealed that the two migratory forms of Caspian salmon were categorized in conspecific value.
    Keywords: Biology ; Fisheries ; Genetic diversity ; RAPD ; Caspian trout ; Fall ; spring run ; Salmo trutta caspius ; Iran ; genetic ; DNA
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 437-446
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