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  • 1
    Electronic Resource
    Electronic Resource
    Genetic Variation Within and Among Rainbow Trout, Onchorhynchus mykiss, Hatchery Populations from Iran Assessed by PCR-RFLP Analysis of Mitochondrial DNA Segments (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 68 (2003), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Samples were collected from 3 hatchery populations of 2 trout inbreeding centers (Kelardasht and Jajrood) and mitochondrial DNA (mtDNA) of these samples was studied by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). ND5/6 segment and a fragment that includes 12SrRNA, tRNA F, P, T, D-loop, and a part of cyt.b of mtDNA were amplified and investigated using different restriction enzymes. Two patterns were observed using HaeIII, AvaII, RsaI, TaqI, and Hinf enzymes on gel electrophoresis, resulting in 9 haplotypes in ND5/6 segment, whereas only 1 pattern was observed for the fragment containing D-loop. As a measure of intrapopulation diversity and interpopulation diversity, both heterozygocity and genetic distance were determined, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2003.tb08258.x
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  • 2
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    Molecular study of the populations of Artemia partenogenetica in Iran using PCR-RFLP Method (2018)
    Details
    Publication Date: 2021-05-19
    Description: Considering the importance of genetic studies to manifest inter population differences in species, samples of Artemia partenogenetica were collected from seven inland lakes including Shoor and Inche-Borun lakes in Golestan Province, Hoze-Soltan and Namak lakes in Qom Province, Maharloo and Bakhteghan lakes in Fars Province and Mighan pool in Markazi Province. A total of 210 samples were subjected to DNA extraction by phenol-chloroform method. Primers were designed on a ribosomal fragment (16SrRNA) of the species' mtDNA sequence and the PCR was conducted on the samples. Digestion of the PCR product with approximately 1584bp lengths by 10 restriction endonuclease (AluI, EcoRI, Eco47I, HaeIII, HindIII, HinfI, MboI, MspI, RsaI, TaqI) showed 12 different haplotypes: 4 haplotypes in Shoor and Inche-Borun, 1 in Namak and Hoze-Soltan, 3 in Mighan pool, 1 in Bakhtegan and Maharloo and 3 in Maharloo. Haplotype diversity values within collected samples varied from zero in Hoze-Soltan, Namak and Bakhteghan samples to 0.7425 in Inche-Borun and Shoor while nucleotide diversity varied from zero in Hoze-Soltan, Namak and Bakhteghan, to 0.0077 in Mighan. The minimum nucleotide diversity among samples was zero between Hoze-Soltan vs. Namak and the maximum was 0.1700 between Inche-Borun and Shoor vs. Mighan. Nucleotide divergences among samples were least in Inche-Borun vs. Shoor (%-0.02) and most in Inche-Borun and Shoor vs. Mighan (%16.18), averaging to %3.40. The evolutionary distances between 12 haplotype showed that the maximum value belonged to Mighan haplotypes vs. Inche-Borun and Shoor haplotypes. Regarding the digestive patterns produced by each enzyme in the studied region, Eco47I is introduced as the population-specific marker of A. partenogenetica in Iran. Test of population differentiation based on haplotype frequencies were statistically significant (P~,0.001) with the exception of Hoze-Soltan vs. Namak and Inche-Borun vs. Shoor. We conclude that there are enough evidences in haplotypic level for dividing A. partenogenetica in Iran into five populations: Hoze-Soltan and Namak, Mighan, Maharloo, Bakhtegan, Incheh-Borun and Shoor.
    Description: Published
    Keywords: RNA ; Nucleotide sequence ; Primers ; Nucleotides ; Artemia partenogenetica ; Population Genetics ; Genetics ; DNA ; Enzymes ; Marine
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.53-68
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  • 3
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    Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay (2019)
    Details
    Publication Date: 2021-05-19
    Description: Caspian trout (Salmo trutta caspius Kessler, 1877) as endemic migratory fish, possessing great ecological economic and biodiversity value. Among the habitat drivers, temperature related effects on the genome were considered in this research. During the embryonic developmental stages, genomic alterations of single cells were assessed by Comet assay. After artificial propagation of wild broodstocks, the batches of eggs, were transferred to incubators with constant temperatures, REDD water recycling, 3 stages water filtration, UV-C application on effluent of each incubator and DO–pH–temperature control. Samples were collected from normal (8°C), cold (4°C) and warm (12°C) treatments on eyed egg, alevin and larval stages. Single Cell Gel Electrophoresis were applied on samples for evaluation of Comet Tail length (TL), DNA in tail (DT) and Tail Moment (TM) indices as DNA damage markers. Comparison between mean values of Comet TL index indicated 85.19 ±9.2, 9.88 ±2.3 and 256.54±14.6 for 4, 8 and 12°C treatments, respectively. DT were 18.07±6.3, 9.72±1.9 and 31.47±2.8 and then TM were measured as 15.27± 4.1, 1.21±0.2 and 95.25±8.6 for 4, 8, and 12°C treatments, respectively. Significant difference between thermal treatments (p〈0.05) observed. Warm treatments demonstrated greater DNA damage in compare with others. TM explained better information in comparison with TL and DT in all analyzed samples. In conclusion, temperatures out of the range of the optimal conditions could significantly affect Caspian trout genome which could be the subject of management considerations pertaining to Caspian trout stocks rehabilitation and captive rearing strategies.
    Description: Published
    Keywords: Ecological ; Caspian trout ; Salmo trutta caspius ; Temperature ; DNA damage ; Comet assay ; Evaluation
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.48-59
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  • 4
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    Effects of incubation and larval rearing temperature on histological changes of muscles in Caspian trout (Salmo trutta caspius Kessler, 1877) larvae (2017)
    Details
    Publication Date: 2021-05-19
    Description: Out of considerable environmental factors, temperature and its possible effects on life stages of Caspian trout investigated by natural incubation (8 ͦC) condition simulation comparing with cold (4 ͦC) and warm (12 ͦC) constant incubation temperatures in 3 well equipped incubators by water recycling systems. Green eggs triple treatments of wild and F1 cultured brooders were incubated. Incubation implemented in dark by using REDD water and DO–pH – temperature digital monitoring ended to yolk sac absorption and entering larval stage. Numbers and diameters of white fiber muscles measured and significant differences considered between three thermal treatments (P〈0.05) in both wild and cultured stocks. The numbers of white fiber muscles in warm treatment by highest means (72.54) and lowest diameter (8.46 micrometer) compared with Cold treatment white fiber muscles diameter (20.59 micrometer) and numbers (50.72) which were the highest diameter and lowest numbers means between treatments. Hatching success stated considerable mortality for cold treatments and 8°C incubator improved the best temperature in wild treatment. Incubation temperature induced significant effect on white fiber muscles stated considerable index for flesh precursor muscles condition which is subject of natural stocks rehabilitation and domestication projects considerations.
    Description: Published
    Keywords: Caspian trout ; White fiber muscle ; Stocks rehabilitation ; Salmo trutta caspius ; REDD ; Temperature ; Domestication ; Muscles ; Histological ; Larval ; Incubation ; Eggs ; Water ; Factors
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.95-104
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  • 5
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    Molecular study of the populations of Artemia partenogenetica in Iran using PCR-RFLP Method (2021)
    In:  http://aquaticcommons.org/id/eprint/23679 | 18721 | 2018-07-18 06:36:39 | 23679 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: Considering the importance of genetic studies to manifest inter population differences in species, samples of Artemia partenogenetica were collected from seven inland lakes including Shoor and Inche-Borun lakes in Golestan Province, Hoze-Soltan and Namak lakes in Qom Province, Maharloo and Bakhteghan lakes in Fars Province and Mighan pool in Markazi Province. A total of 210 samples were subjected to DNA extraction by phenol-chloroform method. Primers were designed on a ribosomal fragment (16SrRNA) of the species' mtDNA sequence and the PCR was conducted on the samples. Digestion of the PCR product with approximately 1584bp lengths by 10 restriction endonuclease (AluI, EcoRI, Eco47I, HaeIII, HindIII, HinfI, MboI, MspI, RsaI, TaqI) showed 12 different haplotypes: 4 haplotypes in Shoor and Inche-Borun, 1 in Namak and Hoze-Soltan, 3 in Mighan pool, 1 in Bakhtegan and Maharloo and 3 in Maharloo. Haplotype diversity values within collected samples varied from zero in Hoze-Soltan, Namak and Bakhteghan samples to 0.7425 in Inche-Borun and Shoor while nucleotide diversity varied from zero in Hoze-Soltan, Namak and Bakhteghan, to 0.0077 in Mighan. The minimum nucleotide diversity among samples was zero between Hoze-Soltan vs. Namak and the maximum was 0.1700 between Inche-Borun and Shoor vs. Mighan. Nucleotide divergences among samples were least in Inche-Borun vs. Shoor (%-0.02) and most in Inche-Borun and Shoor vs. Mighan (%16.18), averaging to %3.40. The evolutionary distances between 12 haplotype showed that the maximum value belonged to Mighan haplotypes vs. Inche-Borun and Shoor haplotypes. Regarding the digestive patterns produced by each enzyme in the studied region, Eco47I is introduced as the population-specific marker of A. partenogenetica in Iran. Test of population differentiation based on haplotype frequencies were statistically significant (P≤0.001) with the exception of Hoze-Soltan vs. Namak and Inche-Borun vs. Shoor. We conclude that there are enough evidences in haplotypic level for dividing A. partenogenetica in Iran into five populations: Hoze-Soltan and Namak, Mighan, Maharloo, Bakhtegan, Incheh-Borun and Shoor.
    Keywords: Biology ; Population Genetics ; Genetics ; DNA ; Enzymes ; Marine ; RNA ; Nucleotide sequence ; Primers ; Nucleotides ; Artemia partenogenetica ; ISW ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 53-68
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  • 6
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    Effects of incubation and larval rearing temperature on histological changes of muscles in Caspian trout (Salmo trutta caspius Kessler, 1877) larvae (2021)
    In:  http://aquaticcommons.org/id/eprint/22101 | 18721 | 2018-02-08 03:06:51 | 22101 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-06-26
    Description: Out of considerable environmental factors, temperature and its possible effects on life stages of Caspian trout investigated by natural incubation (8 ͦC) condition simulation comparing with cold (4 ͦC) and warm (12 ͦC) constant incubation temperatures in 3 well equipped incubators by water recycling systems. Green eggs triple treatments of wild and F1 cultured brooders were incubated. Incubation implemented in dark by using REDD water and DO–pH – temperature digital monitoring ended to yolk sac absorption and entering larval stage. Numbers and diameters of white fiber muscles measured and significant differences considered between three thermal treatments (P〈0.05) in both wild and cultured stocks. The numbers of white fiber muscles in warm treatment by highest means (72.54) and lowest diameter (8.46 micrometer) compared with Cold treatment white fiber muscles diameter (20.59 micrometer) and numbers (50.72) which were the highest diameter and lowest numbers means between treatments. Hatching success stated considerable mortality for cold treatments and 8°C incubator improved the best temperature in wild treatment. Incubation temperature induced significant effect on white fiber muscles stated considerable index for flesh precursor muscles condition which is subject of natural stocks rehabilitation and domestication projects considerations.
    Keywords: Biology ; Fisheries ; Caspian trout ; Temperature ; White fiber muscle ; Domestication ; Stocks rehabilitation ; Salmo trutta caspius ; larvae ; temperature ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 95-104
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  • 7
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    Inheritance of RAPD marker in female grass carp (Ctenopharyngodon idella), male bighead carp (Hypophthalmichthys nobilis) and their F1 hybrids (2021)
    In:  http://aquaticcommons.org/id/eprint/22333 | 18721 | 2018-03-21 22:25:24 | 22333 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-03
    Description: Genetic variation and inheritance of randomly amplified polymorphic DNA (RAPD) markers in female grass carp (Ctenopharyngodon idella) and male bighead carp (Hypophthalmichthys nobilis) and their F1 offspring hybrid have been studied. For this purpose, genomic DNA was extracted from muscle tissue according to phenol-chloroform method, and six decamer primers were used for amplifying polymorphic DNA. Results from 77 produced bands showed that all RAPD bands pattern of parents were present in F1 offspring hybrid, which indicated the high influence and dominance of the mentioned markers. The low difference in polymorphisms in used markers between F1 offspring hybrid and parents shows that parents are heterozygous in some loci, which can cause low difference in hybrids of two distinct genera. Although our results revealed that RAPD markers had a suitable efficiency in distinguishing parents from F1 hybrids, genetic diversity of triploid and diploid F1 hybrid were not detected by introduced primers.
    Keywords: Biology ; Fisheries ; RAPD Marker ; Grass carp ; Bighead carp ; Hybridization ; Triploidy ; Hypophthalmichthys nobilis ; Ctenopharyngodon idella ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 47-56
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  • 8
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    Evaluation of incubation temperatures on DNA damages of the Caspian trout (Salmo trutta caspius Kessler, 1877) single cells through developmental stages by Comet assay (2021)
    In:  http://aquaticcommons.org/id/eprint/26443 | 18721 | 2019-05-06 10:11:09 | 26443 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-24
    Description: Caspian trout (Salmo trutta caspius Kessler, 1877) as endemic migratory fish, possessing great ecological economic and biodiversity value. Among the habitat drivers, temperature related effects on the genome were considered in this research. During the embryonic developmental stages, genomic alterations of single cells were assessed by Comet assay. After artificial propagation of wild broodstocks, the batches of eggs, were transferred to incubators with constant temperatures, REDD water recycling, 3 stages water filtration, UV-C application on effluent of each incubator and DO–pH–temperature control. Samples were collected from normal (8°C), cold (4°C) and warm (12°C) treatments on eyed egg, alevin and larval stages. Single Cell Gel Electrophoresis were applied on samples for evaluation of Comet Tail length (TL), DNA in tail (DT) and Tail Moment (TM) indices as DNA damage markers. Comparison between mean values of Comet TL index indicated 85.19 ±9.2, 9.88 ±2.3 and 256.54±14.6 for 4, 8 and 12°C treatments, respectively. DT were 18.07±6.3, 9.72±1.9 and 31.47±2.8 and then TM were measured as 15.27± 4.1, 1.21±0.2 and 95.25±8.6 for 4, 8, and 12°C treatments, respectively. Significant difference between thermal treatments (p〈0.05) observed. Warm treatments demonstrated greater DNA damage in compare with others. TM explained better information in comparison with TL and DT in all analyzed samples. In conclusion, temperatures out of the range of the optimal conditions could significantly affect Caspian trout genome which could be the subject of management considerations pertaining to Caspian trout stocks rehabilitation and captive rearing strategies.
    Keywords: Biology ; Fisheries ; Caspian trout ; Salmo trutta caspius ; Temperature ; DNA damage ; Comet assay
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 48-59
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