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  • Iran  (15)
  • Fisheries  (8)
  • Astacus leptodactylus  (4)
  • 11
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25726 | 18721 | 2018-10-10 10:00:38 | 25726 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Today, due to population increase and anthropogenic activities together with sewage and agricultural waste water entrance, aquatic ecosystems have been exposed to high pollutions. Phytoplankton is a group of water floating algae that have crucial roles in providing nutrients and oxygen for other organisms, nitrogen and CO_2 fixation. These organisms are considered as primary producers in aquatic ecosystems. They are found in various water habitats all over the world, affected by environmental variables such as pH, light and temperature and used for determination of water pollution degree and quality. Phytoplankton composition and density may be used as a complementary indicator of water trophy state. Phytoplankton communities indicate short and long term variations of aquatic systems. One of the most obvious problems in freshwater ecosystems is algal bloom or over growth of some blue- green algae which can decrease oxygen, and in some cases bring about toxin excretion and fish and human kills. In addition, many of bloom forming cyanobacteria produce secondary metabolites which can create sever poisoning in mammals including human. The genus Microcystis is a key bloom forming cyanobacteria in aquatic ecosystems. Populations of this genus form intense blooms in water bodies that has attracted more attentions in recent years. Various species of this alga have been distributed in stagnant and eutroph freshwater around the world. Microcystis has been defined by genetic criteria such as 16S rRNA molecular sequencing, but its classification in levels lower than genus is unclear and the presence of its classical morphospecies is doubtful. However, this genus creates sever blooms in eutrophic waters all over the world and many species produce toxins. Therefore, identification of its natural diversity in the levels lower than genus has high importance. However, several characteristics of Microcystis morphotypes which are classified a traditional species, actually are present and observed in different regions of the world. At present, they can be considered as morphospecies that belong to one genotype and have similar ecology. These traditional species with definite phenotypic and ecophysiological characteristics cannot be eliminated completely. Their identification is essential for ecological and ecotoxicological studies. Interistic transcribed separator (ITS) gene is a section of genome which is located between 16s rRNA and 23s genes. This gene has more heterogeneity than 16s rRNA; so it is used to identify many genera of cyanobacteria. The Aras reservoir located in the north-west of Iran plays important roles as fisheries, drinking and agricultural water supply and recreational activities in the region. The present thesis was undertaken to: analyze the Microcystis sp. populations by molecular methods such as ITS in Aras Reservoir. Samples for molecular analysis were collected from 10 sampling sites on 18 August 2013. Samples for molecular study of Microcystis were collected from two different depths (surface and 1 m depth) and transferred to laboratory without any treatments. Microscopic images of Microcystis were sent to professor Komárek and was confirmed.
    Keywords: Biology ; Iran ; Aras dam ; Molecular ; Barcoding ; Microcystis aeruginosa ; ITS-2 ; Gene ; Population ; Aquatic ; 16S rRNA
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 44
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  • 12
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25749 | 18721 | 2018-10-13 07:31:35 | 25749 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: After the outbreak of various viral diseases, specifically white spot syndrome (WSS) in farmed Litopenaeus vannamei in the past few years, this non-native shrimp species in Iran requires a generator-builder with specific a pathogen-free approach. This Research was done by the Iran Shrimp Research Center (ISRC) to access accurate information regarding specific pathogen-free shrimp production and avoid using shrimp broodstock production through foreign investors. This research was part of the molecular viral study. For this trial, selected shrimps in farms were sampled and screened for main viruses (OIE list). Shrimps without infection were brought to the quarantine system for one month and at the end of the quarantine they were screened again. In addition, in winter spending, before and after spawning, it’s offspring in F1 and F2 were also screened for viral pathogens by IQ2000 kit at the Iran Shrimp Research Center lab and Iranian veterinary organization lab. All shrimp products such as fresh and consumed food were tested for OIE list at all stages during project performance and the result of tests were negative. Sequenced and molecular tests with specific primers were used to determine the presence of infected samples carrying the virus in any of the products, such as shrimp and consumed food, the infected samples. The result of all the tests during last three years, shows that all the shrimp and their food products were pathogen-free and safe to use.
    Keywords: Biology ; Iran ; Shrim ; SPF ; Virus ; White Spot Syndrome Virus ; WSS ; Spawning ; Molecular
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 48
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  • 13
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    In:  http://aquaticcommons.org/id/eprint/22516 | 18721 | 2018-04-16 21:36:31 | 22516 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-06
    Description: Fish Protein Concentrate (FPC) is a healthy, sustainable and high nutritive product that produced from fish and protein and other nutrients are more concentrated than in the fresh fish. The aim of this research is to study the sustainability of FPC produced from Kilka (combination of three Caspian Sea Kilka species, Clupeonella engrauliformis, C. grimmi and C. cultriventris which were not identified and processed separately) in VP (Vacuum Packaging) and MAP (Modified Atmosphere Packaging) at different temperatures during six months of storage. According to result of chemical analysis performed, protein content was evaluated 91.2%, lipid 0.5%, ash 3.6%, moisture 2.3%, TVN 10 mg/100g and peroxide 5 meq/kg in the produced FPC before packing. Amino acids and fatty acids were also determined. Lipid amount in FPC after 6 months at 35°C in VP changed from 0.50 to 0.45 and in MAP (combined of 60% CO2, 30% N2 and 10% O2), decreased from 0.50 to 0.36. It was also detected that increase in temperature leads to more decrease in lipid content but it was not significant (P〉0.05). Protein content of FPC has changed from 91.2% to 73.6% during six months at 35°C in VP and 69.4% in MAP. But at 5°C, protein contents were changed from 91.2% to 88.4% and 81.2% in VP and MAP, respectively; these changes were significant (P〈0.05) but the decrease in MAP was again more than VP.
    Keywords: Biology ; Chemistry ; Fisheries ; Fish Protein Concentrate ; Kilka ; Vacuum Packaging ; Modified Atmosphere Packaging ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 338-346
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  • 14
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    In:  http://aquaticcommons.org/id/eprint/24722 | 18721 | 2018-08-04 16:30:26 | 24722 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-15
    Description: Anzali Lagoon is one of the most important aquatic ecosystems of Iran which was registered as a Ramsar Convention site in 1999. This valuable ecosystem is located in the south west shores of the Caspian Sea, in Guilan Province. We randomly collected 30 and 20 samples of the bivalve Anodonta cygnea in autumn 2004 and spring 2005 respectively. The Selkeh area was chosen for the sampling because of its availability during autumn. The area receives water from the southern part of the lagoon basin. Nutrient content of the soft tissue of the bivalve was measured. Live sampled bivalves were transferred to laboratory and their length, width, height; total body weight and wet weight of the internal soft part were measured. Moisture, ash, protein, T.V.N, lipid and amino acid contents in soft tissue were also determined. Moisture content in spring and autumn samples were 84.84% and 83.6%, respectively. Ash content in autumn samples was higher than spring samples, being 4% and 2.32%, respectively. Assessment showed protein content in the autumn samples to be 12% while in spring samples this was 10.5%. T.V.N content in autumn and spring samples were 0.031 and 0.003% respectively. Measurements showed that autumn bivalves had 4% lipid content whereas this value in spring samples was 3%. We found Sixtheen amino acids, including seven essential ones in the samples.
    Keywords: Ecology ; Nutrient values ; Anodonta cygnea ; Anzali Lagoon ; Caspian Sea ; Guilan Province ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 9-22
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  • 15
    Publication Date: 2021-07-16
    Description: The comparative study of the streptococcinum, Hepar sulfur, Rosmarinus officinalis and erythromycin effects on cultured rainbow trout (Oncorhynchus mykiss) with experimental streptococcosis Homeopathy is one of alternative medicines that is very useful for soul and body diseases with accurate prescription. The goal of this study was “survey of the effects of homeopathic remedies. There is not any more research about homeopathy on aquatics in the world especially in Iran, thus Some research about the effects of homeopathy on aquatics is needed. In this study, the effects of streptococcinum, Hepar sulfure, Rosmarinus officinalis(homeopathic remedies) and erythromycin in cultured rainbow trout, with experimental streptococcosis, also the mortality, were compared. There was 6 treatment and 2 reviews in 300 liter tanks that each of treatment contained 40 juvenile rainbow trout with 25±5 gr arrange weight. Treatment 1: contained of erythromycin. Treatment 2: Streptococcinum C30. Treatment 3: Rosmarinus officinalis Q. Treatment 4: Hepar sulfur C30 . Treatment 5:(control treatment )without any injection and any therapy. Treatment 6: (positive control treatment) with injection but without any therapy. Daily estimation of the water temperature, oxygen, pH and salinity and some other chemical factors. Tretment 1 had significance with the other treatments. Survival percent in treatments and their analysis showed that treatment 4(Hep-s) had significance (p〈0.05) with treatment 3(Ros-off) and its survival percent is more than the other homeopathic remedies. Erythromycin is chemical drug and has many side effects but Hep-s has not any side effect and is an natural remedy for Streptococcosis in homeopathy. Thus we offer the Hep-s to cure the streptococcosis but some research with disk diffusion test about the different doses of Hep-s is needed. Daily survey of clinical symptoms such as hemorrhages in the external organs, Under eyes, under skin, under fins and gills, hemorrhages and exophthalmos, were the most symptoms. Important pathological symptoms were: necrosis, hyperplasia and melanosis in branches, liver and kidneys, hemorrhages in heart, kidneys and in visceral tissues. According to the survival results, there was significant difference between the treatment 1 and the other treatments (p〈0.05). Also there was significant difference between treatment 3, treatment 4 and treatment 2(p〈0.05), this difference is due to the high dose(Q) of R.officinalis, while the two other homeopathic remedies were in a moderated dose (C30), 30× 100 diluted dose. Survival percent of treatment 4(Hep-s) was more than the other homeopathic remedies and was related to erythromycin. Erythromycin is chemical medicine and has many side effects, while Hepar sulfur has not any side effects if the prescription would be accurate. Hep-s is suggested with disk diffusion tests for relief the symptoms of streptococcosis.
    Keywords: Aquaculture ; Health ; Iran ; Homeopathy ; Oncorhynchus mykiss ; Hep-s ; Ros-off ; Streptococcinum ; Erythromycin ; Streptococcusis ; Rainbow trout
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 38
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  • 16
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25600 | 18721 | 2018-10-07 16:21:40 | 25600 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Astacus leptodactylus is an important economical fisheries resource of Aras dam reservoir, in Iran. The infection of freshwater crayfish by gram negative and gram positive bacteria is often observed in natural and cultural environments which are considered as secondary disease agents or opportunities. This study was conducted to investigate infection conditions on 70 crayfish from the Aras reservoir regarding the bacterial agents by culturing from hemolymph in aseptic condition on Blood Agar, Trypton Soya Agar (TSA) and Cytophaga Agar The study was performed by biochemical tests. In this study Aeromonas hydrophila, Staphylococcus aureus, Micrococcus luteus and Flavobacterium johnsonaewere isolated and determined. According to the results of this study which was performed for first time on Aras reservoir freshwater crayfish, the percentage of crayfish which carried bacteria in hemolymph were variable ( 20-58.5%). Also, the gram negative bacteria particularly Aeromonas hydrophyla were dominant among the bacteria isolated from studied crayfishes.
    Keywords: Health ; Iran ; Aras dam reservoir ; Freshwater ; Crayfish ; Bacterial infection ; Aeromonas hydrophila ; Staphylococcus aureus ; Micrococcus luteus ; Flavobacterium johnsonae
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 32
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  • 17
    Publication Date: 2021-07-24
    Description: The aim of this study was to determine the qualitative characteristics of ready-made fish paste made from minced meat (Clupeonella cultriventris) and silver carp (Hypophthalmichthys molitrix) and its shelf-life at a refrigerated temperature.This compilation was performed in five treatments including amounts 100, 75, 50 and 25 percent of processed fish meat were cured. The treatments were packed in heat-resistant glass after pasteurization. Samples were stored at 4oC for 40 days.The amount of TVB-N in the experimental treatments during the course of the change was increased that this factor varied between15.25-31.25 mg/100 g of meat in experimental treatments. This factor was in the 100% rolled silver carp treatment until the end of the shelf life in the standard range. The amount of tybarbibacteric acid increased during storage period varied between 0.13- 2.11mg malondialdehyde/1000g in experimental treatments, but this factor was in the 100% treatment of silver carp paste after the end of the maintenance period in the standard range (less than 1.8 mg/100gr), in other treatments, after 30 days, was more than standard limitation. The pH changes in treatments did not significantly decrease (P〉0.05), this factor varied between 5.05 to 5.93 in the experimental period. Total bacterial counts, psychrophilic bacteria and coliform and molds were not observed in experimental treatments during storage period. Microbial contamination was not observed during the storage period in the refrigerator in the experimental treatments. The taste and texture in silver carp treatment (100%) were better than other treatments. The ash, protein and fat in 5 treatments did not significantly change during storage (p〉0.05). The moisture content was not significantly increased in all five treatments during storage (p〉0.05). Results showed treatment of processed carp (100%) was better than other treatments (p〈0.05).
    Keywords: Aquaculture ; Fisheries ; Iran ; Sensory evaluation ; Fish paste ; Silver carp ; Minced meat ; Kilka ; Qualitative ; Clupeonella cultriventris ; Hypophthalmichthys molitrix
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 111-120
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  • 18
    Publication Date: 2021-07-16
    Description: Regarding the increase in world population and decrease in fish stock, there is an urgent need to aquaculture practices. Coldwater fish culture is aiming at production and providing a part of protein needs of the country and a way reaching to self- sufficiency and preserve the water resources. Recently, multi- purpose use of water resources potential and especially the rivers of west Azarbaijan, for example, construction of fish farms at upstream of permanent rivers in order to make optimum use of the resources and the increase in employment has been considered . The waste water of these pools is usually discharged into the rivers. Regarding to increased production per unit area due to water resources and cold water fish culture restrictions to use regional facilities and fish farm construction, it is nessacery to determine limits between 2 farms. In this study with the goal of evaluation of the impacts of trout farm effluents on water quality parameters of Pirdanan River, the factors of water current, temperature, pH, EC, TDS, TSS, DO, BOD_5, NH_3, N-NO_2, N-NO_3, TP P-PO_4 according to standard methods were determined montly during 2014-2015 . Sampling sites were entrance, exit of Maroofi trout farm and the distances of 700, 1400, 2100 and 2800 m after the farm exit. Seasonal sampling of macro invertebrates from mentioned sites was conducted using stone leaching. The family and genus of macro invertebrates were identified using identification keys and abundance of macro invertebrate phylums for each site was determined. The results revealed that the values for water current, EC, TDS, TSS, N-NO_3 were significant. the values of water temperature, BOD5, TDS, TSS, N-NO^3- , N-NO^2-, NH_3, P-PO^4- , NH4+, N-NH3 were increased in site 2 compare to site 1 in all months and gradually in the river and its self- purification were decreased. In this study, 4 phylums, 4 classes, 7orders and 14 families of macroinvertbrates were identified from Pirdanan River. Ephemeroptera had the highest abundance in site 4; while Diptera had the highest abundance in site 2 (exit). Regarding that most of Diptera macro invertebrates were from Chironomidae, so it can be concluded that site 2 was the most pollutant site and sites 5 and 6 were more suitable condition. Therefore, considering the physic- chemical factors and macro inverteberates indices, 2100 m from entrance, (site 5) is recommended compliance with veterinary organization rules and other conditions of aquatic engineering, environmental and water operation standards.
    Keywords: Aquaculture ; Ecology ; Management ; Iran ; West Azerbaiejan Province ; Pirdanan River ; Onchorhynchus mykiss ; Culture ; Water quality ; Permitable distance ; Macroinvertbrates ; Coldwater ; Common water
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 54
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  • 19
    Publication Date: 2021-05-19
    Description: Aras reservoir freshwater crayfish is an important economic fisheries resource of West Azarbaijan, Iran. This study was concluded to evaluate the prevalence of parasitic infestation of Crayfish in this area during different seasons of 2012. Among 390 different sizes of Astacus leptodactylus which were examined, arange of ectocommensals or ectosymbionts from a number of different phyla including 9 phylm and 11 class infested the different anatomic units of the surface and appendages such as gills, head , thorax, abdomen, walking legs, uropod, telson, antennae and antennulae of freshwater crayfish. Common groups such as peritrich ciliates, suctorian ciliates, free living nematodes, branchiobdellids, and Algae, copepods, rotifers and oligochaetes have also been observed in association with freshwater crayfish.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Crayfish ; Freshwater ; Parasitic andcommensal infestation ; Astacus leptodactylus
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 58pp.
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