ALBERT

Description: In order to introduce a new sturgeon hybrid to aquaculture industry, the possibility of hybrid production by crossing between Siberian and Persian sturgeons and its comparison with its pure parents using morphometric, molecular, cytogenetic and growth performance were conducted. For this purposes eggs and sperms of two pairs of Persian and Siberian sturgeon were collected and reciprocal crosses with 4 treatments were conducted. In treatment 1, Persian sturgeon as control (male and female of Persian sturgeon), treatment 2 (as type I hybrid) from crosses of male Persian sturgeon with eggs of female Siberian sturgeon in treatment 3 (type II hybrid) using crosses between male Siberian sturgeon and female Persian sturgeon and finally treatment 4 by crossing male and female of Siberian sturgeon were used. For each treatment 200 gr. of eggs were fertilized with one ml. of sperm and morphological, meristic parameters of parents and offspring were recorded. For growth comparison of fingerlings of control and hybrids in 3 different phases were measured. In phase (I), larvae rearing from 46 mg. up to 6600 mg. were conducted for a period of 10 weeks. Samples were collected once every two weeks and biological parameters were recorded. In rearing phase II for a period of 330 days, growth comparison were conducted for fish from 6.5 gr. to 750gr. and biological parameter were recorded once every month, where daily growth rate (gr./day), FCR, Specific growth coefficient (percentage per day), increase of body weight, food efficiency and protein efficiency ration were analyzed. In rearing phase (III), from 600 gr. to 2000 gr. for a period of 43 weeks, similar to phase (II) all biological parameters were analyzed, where 26 morphometric and meristic parameters of pure and hybrid parents and offspring were compared both for individual and ratio status of parameters. For genetic analysis and variation between control and hybrid specimens the microsatellite analysis were conducted. For this purposes, genomic DNA were extracted from fin tissues of brood stocks and fingerlings and 10 pairs of specific primers (Afug 56, Afug 63, Afug 241, Afug 229, OX 27, Afug 686, Afug 195, Afug 12, IS 68 and Afug 160) were used. Then PCR Products were electrophoresed on 6% polyacrylamide gel and then were stained with Nitrate silver and alleles were evaluated based on its length (base pair). Cytogenetic analysis and number of chromosome of control fish and hybrid were compared using white blood cell culture method and chromosome spread of parents as well as offspring were prepared by Gimsa staining and were visualized under light microscope. For sexual gonad development, laparoscopic method with light camera (Model M-CAM 1700) were used to differentiate 34 pieces of control and hybrid fish and gonad status of ovary and testis were analyzed. In present investigation the results indicate that, in phase (I) of larvae culture, a significant differences were observed after 10 weeks of feeding (p≤ 0.05) in hybrid and control groups. Maximum growth rate were observed in Siberian sturgeon larvae, followed by type I hybrid, type II hybrid and Persian sturgeon larvae. In phase (II) , growth rate of Persian sturgeon were lower than Siberian sturgeon. For period of 330 days of rearing periods, the control Siberian sturgeon had 1.4gr. weight increment per day, while Persian sturgeon had only1. gr./day. However the hybrid type (I) had an average daily growth of 1.5gr/day and lower growth performance were observed in type II hybrid with daily growth of 1.5 gr./day. In phase (III), growth rates were varied at different age group. The daily growth rate of Siberian sturgeon was 4.5gr/day, while is control Persian sturgeon was 2.6 gr/day. The Siberian sturgeon within 47 weeks of rearing grown from 655 gr. to 2016 gr., however Persian sturgeon for the same period reached from Siberian sturgeon with male’s Persian sturgeon had higher type II hybrid showed opposite results for the same growing period and reached from 640 gr. to 1680 gr. In conclusion of growth performance can be stated that the hybrid from crosses between female Siberian sturgeon with male Persian sturgeon presented highest growth rate in comparison to control hybrid. This excellent achievement can be considered as a big step toward sturgeon aquaculture for meat and Caviar production. In analysis of morphometric characters 26 parameters were investigated which in 18 parameters showed significant differences (p≤ 0.05) which indicate clear distinguish able of hybrid performance in compare with parents where Siberian sturgeon has longer snout compare to Persian sturgeon and its size are intermediate in hybrids. In relative ratio of characters, out of 9 characters totally 7 characters showed significant differences (p≤ 0.05). In molecular analysis using microsatellite primers totally 400 DNA samples of hybrid as well as the control fish were investigated. Totally 784 allele were detected in which 363 alleles were belong to control fish and 421 alleles in hybrid. In this study, 19 alleles with different sizes were observed in hybrids rang from (120bp) in Aox27 primers (400bp) in AFUG229 primers. In comparing of all primers, the primer AFUG160 and AFUG195 with 2 alleles and primer AFUG686 with 7 alleles showed the minimum and maximum allele numbers.The banding patterns were monomorphic in 66 loci of control fish and 65 loci of hybrids, and in 77 loci of control fish and 73 loci of hybrids were diploid in 9 loci of control fish and 12 loci of hybrid fish were tetraploid, in one locus of control samples and 3 hybrid samples were pentaploid and finally within 10 pairs of primers 7 sets were disomic, 3 primers (Afug241, Afug686, Aox27) were tetraploid and 6 primers of (Afug63, Aox27, Afug688, Afug12, Is68, Afug160) showed excellent allele heritability which indicate the high efficiency of microsatellite techniques. In evaluation of the status of gonad development as well as determination of sexual stages laprascopic method indicated that out of 9 samples of Persian sturgeon (4 females and 5 male) were in stage of 2 & 3 gonad development. Out of 8 Siberian sturgeon (4 males+4 females) stage 2, 3, 4 were detected. In 7 gonad samples of hybrid (Female Persian and Male Siberian) totally 5 male and 2 female were observed at stage 3 and 4 gonad development. In analysis of 10 samples of type II hybrid (female Siberian × male Persian) 3 female, 6 males and 1 deformetic were observed. Cytological studies on 30 metaphase palate from 6 fish samples showed that the number of chromosome in hybrid are as same as parents 2n=250±10 where all control and hybrids similar to its parents showed 240 chromosome. In conclusion, It can stated that, this study successfully introduced a new sturgeon hybrid for sturgeon farming both for meat and caviar production. Considering the common name of sturgeon parents used for production this hybrid, we call this hybrid as (Siper) where Si instant for Siberian sturgeon and Per comes from Persian sturgeon.

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: In order to evaluate the possible production of hybrids using two species of sturgeon; beluga (Huso huso) and Persian sturgeon (Acipenser persicus) a reciprocal crosses with three treatments and three replicates for each treatment was conducted. Reproduction normatives including number of eggs per gram, fertilization rate, survival rate, and also 32 mophmetric and merestic parameters of parents, hybrids and control groups were compared. Genetic analysis of hybrid was conducted using two methods of cytogenetic (chromosome preparation) and molecular (microsatellite) techniques. Histological analysis was performed for sexual gonad development. The growth comparison between hybrids and control fish was conducted in fiberglass tanks for 18 months. Fish were fed using pellets and biometric measurements were carried out 17 times during the study period. Means, analysis of variance, standard deviation, Duncan s test and percentage of hetrosis were calculated using Quatro Pro and SPSS programs. Significant differences were detected between beluga controls and hybrids (male beluga x female A. persicus) and between A. persicus controls and hybrids (male beluga x female A. persicus) regarding number of eggs per gram (P 0.003). However no significant differences were detected between the control groups and hybrids regarding fertilization rate at the four celled and 35 celled stages, number of larvae produced, mortality rate up to the onset of exogenous feeding and the number of larvae surviving (P 0.01). Growth rates differed in hybrid fish and fish in the control groups and highest weight increase at the end of the rearing period belonged to beluga control (975 ± 10 g) followed by hybrids produced by crossing female beluga with male A. persicus (840 ± 143 g), hybrids produced by crossing female A. persicus with male beluga (681.85 ± 281 g) and lowest growth increases belonged to the A. persicus control group (535.15 ± 131 g). Specific growth rate in the second and third six months of rearing in hybrids produced by crossing female beluga with male A. persicus was higher than those recorded in the beluga control group. Percentage of hetrosis was negative during the early rearing period (-18.93), however at the end of the rearing period offspring were superior to parents and percentage of hetrosis was 0.79. Comparison of 32 morphologic and merestic parameters showed significant differences between 23 parameters between beluga controls and hybrids and between 31 parameters between A. persicus controls and hybrids (P 0.05). The hybrids production was proved using the cytogenetic (chromosomal count) as well as microsatellite techniques. The number of chromosomes in hybrids was intermediate to the parents (2n =190 ± 9) and like all other sturgeon species, microchromosomes comprised more than 50% of the chromosomes. The chromosome number in hybrids was half the number of chromosomes in the parents (A. persicus 2n=258±4 and beluga 2n=118±3). With regard to the fact that the number of chromosomes in A. persicus is 4N and that in beluga is 2N the number of chromosomes in hybrids is 3N or triploid. DNA bands produced by PCR in parents and offspring showed genetic inheritance. Histological analysis of control fish and hybrids after 18 months of rearing showed that male and female cells were observed in hybrids that is a characteristic feature of impotent or sterile fish. However only one type of sexual cells were observed in fishes in the control groups (A. persicus and beluga). Results obtained from the present study show that the hybrids produced are triploid (3N) and histologically sterile. Also hybrids produced showed good growth. With regard to the scarcity of female beluga and the limitation in the production of beluga fingerlings, it is suggested that sturgeon hatcheries produce hybrids and thus meet the fingerling demands of sturgeon farms. Also considering that the hybrids produced are sterile they can be considered as a candidate for export for aquarium fish. With regard to the fact that the hybrids produced are a new species it is suggested that this species is named Belupars which is a taken from the names of the two parents Beluga and Persicus .

Description: Iranian Fisheries Science Research Institute

Description: Published

Description: This study was conducted to codify the guideline and indexes of broodstock management in salmonid fish and cryopreservation, selective breeding and sex reversal in hatchery to attain responsible aquaculture development. Cryopreservation includes of sperm and egg in term of economic is important in aquaculture. In this report mentioned the type and name of material. All female and sex reversal fish are produced by steroid hormone which mentioned in this report. Selective breeding include individual selection and family selection which could be used for genetic improvement of fish.

Description: The lack of sustainable management programs has resulted in the drastic decline of sturgeons stocks in the Caspian Sea in the past two decades. Legal catch quotas for all Caspian littoral states has dropped from 28500 tons in 1985 to 460 tons in 2007 while caviar production in the Caspian Sea during the same period decreased from 3000 tons to 70 tons. Caviar production in the I.R. of Iran dropped from 305 tons in 1985 to about 11 tons in 2007. The "Strategic and applied research planning for sturgeon management and conservation" was developed with the collaboration of academicians from universities, researchers, experts, fishery authorities and representatives of the executive government to maintain sustainable development and rational management of sturgeon stocks in the Caspian Sea. The preparation and development of this planning was carried out through 121 sessions (2100 person hours) A comprehensive report (375 pg) was prepared which was divided into five volumes; Volume I comprises an introduction, objectives, strategies and planning, present status of production and caviar harvest, catch and export quotas, revenue generated and release of sturgeon fingerlings, Volume II comprises analysis of the problem by constructing a problem tree with 8 broad categories to analyze 344 problems and an objective tree which is the hierarchic flowchart of objectives with 9 broad categories with 241 items, Volume III comprises a review and analysis of previous and ongoing research (414 projects) on different aspects of sturgeon during the past 40 years, Volume IV comprises prioritizing research objectives outlined by the objective tree and finally Volume V which prioritizes primary objectives for 14 executive and 10 research programs. On the basis of the evaluation of the present status and for the sustainable development and rational management of sturgeon stocks a strategic and applied research planning program was proposed and developed within the framework of three primary objectives; 1) Management and sustainable use of Caspian Sea resources (5 programs), 2) Rehabilitation and restoration of stocks (5 programs), 3) Development of aquaculture (4 programs). The applied research program for each proposed executive program was outlined in 10 programs, 42 comprehensive plans and 222 projects. The primary objective on management and sustainable use of Caspian Sea resources includes 5 programs, 14 comprehensive plans and 63 projects, while the primary objective on rehabilitation and restoration of stocks includes 4 programs, 16 comprehensive plans and 87 projects. The primary objective to address sturgeon aquaculture includes 1 program, 12 comprehensive plans and 72 projects. The impacts and outcome of each of these programs was determined and presented. It is evident that if the present situation persists, the catch figures for adult sturgeon specimens and caviar production in Iran will reach zero in 2021. But if concerted efforts are taken and the proposed strategic and applied research planning program is executed (commencing from 2009) we can put a halt to these declining trends. By producing sturgeon fingerlings to restore population abundances, by conserving and protecting them in the Caspian Sea and by conducting applied research we can produce 206.4 tons of caviar by the year 2033. Apart from harvesting caviar from the Caspian Sea we can also annually produce 3000 tons sturgeon meat and 60 tons farmed caviar starting from the year 2023. The total budget proposed for implementation of this program for a period of 14 years (2008-2022) is USD 2483 million. If this budget is allocated in time and the proposed strategic program is properly and totally implemented, we can not only save many sturgeon populations from extinction but also ensure job opportunities for 6000 fisherman, provide 1480 new job opportunities and annually produce 266 tons of caviar which will generate USD 4957 million.

Description: The objective of the present study was to determine the possible production of Persian sturgeon (Acipenser persicus) and Beluga (Huso huso) gynogen/triploids and also to determine the most appropriate type of thermal shock and the duration of induced shock after fertilization. Persian sturgeon and Beluga spawners were collected from Guilan's sturgeon catch stations and transported to the Shahid Beheshti sturgeon hatchery for artificial breeding and restocking programs. Ovulated eggs and sperms were collected based on common procedures in hatcheries. In order to separate the seminal fluids and dilute the milts, sperms were centrifuged at 6000 rpm for 20 min. and seminal fluids stored in refrigerator for further use. Sperm motility was investigated. In order to determine the best duration for radiation, the milt was diluted (1:9) with immobilizing solution. Samples of diluted milt were placed for UV irradiation (UV lamp model UVG-54, 254 nm, made by UVP America) for 0.5, 1, 1.5, 1.45, 2, to 5 min. The motility of radiated sperms and controls were examined under the light microscope and the motility curve was drawn. For application of thermal shock two types of heat shock (32, 34 and 37°C) and cold shock (0±1°C) were used for duration of 2.5 and 60 min respectively. Both thermal shock were applied at 12, 15, 18 min after fertilization. Four experimental groups were designed including; normal eggs as control group and sperms without UV thermal shock), gynogenesis (Sperm irradiated with UV and thermal shock were applied), triploid (thermal shock without radiation by UV on sperm) and haploid group (without thermal shock but using irradiated sperm for fertilization). Verification of the success of treatments was assessed using genetic analysis on sturgeon larvae and fingerlings. In triploids the total surface area, volume of cells and nucleus as well as chromosome number were determined. To identify a gynogenetic larva, microsatellite markers were used to analysis specific loci by using primers designed for lake sturgeon. The results were analyzed using SPSS, Excell software. To determine the significant levels between various parameters and comparison between controls and various treatments, one way of Analysis of Variance (ANOVA) was used. Whenever the significant level was observed to determine its level a Duncan test were examined. Results of present study showed that the best duration for UV radiation on sperms of Beluga was 105-110 seconds. Average fertilization rate for control Beluga was 51%, while in heat shock group it was 2-5 % and in cold shock it was 44.6%. There was a significant difference in fertilization rate in cold shock group compared to heat shock group (P〈0.05), however no difference was observed between 32 and 34°C treatments. The average survival rate of larvae in control group was 51%, while in heat shock treatment (32 and 34°C) it was very low close to zero. However in cold shock treatment the results was better and hatching percentage of larvae was between 30 -35%. Triploid treatment showed better results than gynogenesis group. A minimum triploid larvae obtained from heat shock was zero but using cold shock, the maximum number of 170 specimen was harvested. There was no significant difference in the number of larvae obtained between 32 and 34° C treatments (P〈0.05). Although some difference was observed on large and small axes, surface areas and volume of red blood cells but no significant differences were observed between control and triploid groups (P 0.05). In the meantime, the chromosome number in triploid beluga was (3N=177±3) as compared to diploid 2N= 118±3, which indicated an extra set of chromosome (n=60) in triploid fish. Totally 26.6% of investigated fish was triploids. Microsatellite molecular markers clearly differentiate gynogenetic fish on the bases of allele inheritance of male and female parents, and were proven that this technique can clearly identify allelic inheritance of parents to offspring. In Persian sturgeon in compare to beluga a different results were observed. Heat shock (37°C) not present any positive results therefore has no application in induce gynogenesis on this species, also no significant difference was observed between 32 and 34°C treatment. Cold shock showed better results, especially when duration of UV radiation was adjusted to 105 seconds. Molecular analysis using microsatellite marker positively proved the gynogenetic offspring by counting the allelic inheritance. However Persian sturgeon as a tetraploid species (2N=240) has its difficulty on scoring the banding patterns. We highly recommend disomic primers application for allelic inheritance on gynogene Persian sturgeon.

Description: Based on an agreement between Jahad Nasr Hamzeh Company as "the employer" and the Iranian Fisheries Research Organization as "the executor", a project entitled “culture of Huso huso up to 10 kg" was conducted. A total of 600 Huso huso with the average weight of 5000 g were distributed in four 50 m^2 circular concrete tanks (4 diameters) with the density of 25 kg/m^2 . The experimental system was equipped with aeration. Dissolved oxygen concentration was maintained at 6-7 mg/l. Fish were fed experimental diets composed of 43% protein, 22 % lipids, 16.2% carbohydrates and 22 MJ/kg energy for 12 months. At the end of the first rearing phase (365 d), fish with the mean specific growth rate (SGR) and feed conversion ratios (FCR) of 0.27 and 2.91, respectively reached a weight of 9.06 kg. The highest growth parameters and the best FCR were recorded in spring and autumn. The mean body weight increase (BWI %), specific growth rate (SGR) and feed conversion ratio (FCR) in these seasons were estimated at (0.48, 0.36 % per day), (27.4, 20.13 % during the rearing period) and 1.85 and 1.74, respectively. Results obtained from this project revealed that Huso huso is a prospective candidate for intensive culture in concrete tanks and these results can be developed in many parts of the country.

Description: In this study, two population of Molokaei and High health were selected as zero generation of Litopenaeus vannamei broodstocks. After mating males and females adult within inbreeding and crossbreeding three various storage were produced: H♂ × M♀, M♂ × H♀ and H♀ × H♂. The second generation of shrimp were obtained from storage of broodstocks (MH) first generation. The aim of this study was investigated close relationship between different generations of specific pathogenic free shrimp. The analysis d-loop region of mitochondrial genomes of different generations shrimp was observed 997 positions with 3-6 and 766-799 of haplotype and monomorphic, respectively. Despite haplotype diversity and nucleotide diversity of all generations SPF shrimp were 0.877 and 0.12, respectively. But, results showed homozygosity rate is increasing from one generation to next generation, so low genetic distance were observed between different generations of SPF shrimp certain disease-free shrimp. Regarding the results of in this study can be concluded that lack of arriving new broodstocks from outside the country and was mating between adult males and females relative (full-sib) addition to small effective population size there was both genetic drift and number of haplotypes was gradually reduced from zero generation to second generation. Hence, due to the high close relationship between different generations shrimp evaluation of d-loop region of the mitochondrial genome were little genetic difference between them

Description: In this study, after determining history of origin entering white leg shrimp of broodstocks at hatcheries of country, finally, two population of Molokaei and High health were considered as shrimp broodstocks of specific disease free (SPF) zero generation. After inbreeding and cross breeding among adult males and females of two population above, three storage different were produced in the first generation: H♂ × M♀, M♂ × H♀ and H♀ × H♂. The second generation were produced from selective breeding of M♂ × H♀. The aim of this study was to determine genetic differences between different generations of specific pathogenic free shrimp by evaluation of 16S rRNA region mitochondrial of different generations. Result of this study showed that of 486 sites have been identified 484 sites were conserved. Also, monomorphic sites ranged between 482 - 486 sites and was consists 2 polymorphic sites and 2 transitional sites. The number haplotypes, haplotypes diversity, nucleotide diversity revealed in this region were 2, 0.356±0.159 and 0.00147, respectively. Due to the high genetic identify and reduce of genetic distance of shrimp between different generations of SPF shrimp, genetic differentiation and gene flow (Nm) between them were -.142 and -2.00, respectively. This rates clearly shows a low level of polymorphism in this region of mitochondrial of different generation of SPF. This may be occurred because of small effective populations and genetic drift. Thus, offspring had inherited mitochondrial genome from your mother, therefore, it is expected that rate of variation in this genome is influenced by several factors, including genetic drift, due to unavailability of new-broodstock from different areas. It was observed that due to high conservation sites in 16S rRNA region of mitochondrial genome, nothing genetic differentiation was observed among different generations of SPF.

Description: Seaweed belonged to baseborn marine plants with cell wall containing of valued polysaccharides such as Agar, Alginate, Limen sulfate and Carmine, used as food additives and in different industries. Base on previous studies, more than 1000 MT. of Sargassum seaweed withdraw from Oman Sea in Sistan and Baluchistan coastal line which can be collect, dried and powdered for using as supplement in shrimp feed. In this project, Sargassum ilicifolium collected from 6 coastal areas, rinsed, dried, powdered and measured the nutritional values in laboratory for surveying statistically. According to the high nutritional value of Tis coastal seaweed, this variety seaweed powder, replaced with protein resources (fish meal and Soy and Wheat) of white-leg shrimp feed which was formulated by Havorash feed factory of Boshehr in four treatments (A: as control without any replacement) B: with 5%, C: 10 % and D: 15% seaweed replacement, each with three replicates in order to obtain isonitrogenus 33% CP., and iso-caloric (13% fat and 15% carbohydrate) feed using winfeed software. The weighed milled ingredients were carefully mixed using a laboratory food mixer. The mixtures were primed with 30% hot water to yield a suitable pulp. Wet diets were made into 2 mm pellet size and dried at 40 °C in a drying cabinet and maintained in standard condition which was used for water stability and absorption capacity test of the pellets in sea water, statistically one way- ANOVA. The Tis coastal seaweed with 9.8% CP, 2% lipid and 23% carbohydrate had higher nutritional value compared to the other gathered seaweed. Also amino acid and fatty acid profiles, vitamins and minerals were measured in all seaweed samples each, with three replications. As result, for using Sargassum ilicifolium as vitamins and mineral premixes in white- leg shrimp feed, Zinc, Cobalt and Phenylalanine with dose of 1.1, 06.0 and 4.0 ml. 100% dried seaweed must be added respectively. The water stability of D feed treatment in seawater (98%) and C (97%) had statistical differences with A and B (95% stability) (P〈0.05). Water absorption capacity of feeds after one hour immersion in seawater showed significance difference between D (110%) and three others, C(100%), B(85% and control(80%) (P〈0.05). As final aim of this project, enriched product of this seaweed as minerals and vitamins supplements were handsel joinery in the Second Medicine Plant Festival, 2016 and registered in recording organization to give the final certificate.

Description: In order to evaluate the possible production of hybrids using two species of sturgeon; beluga (Huso huso) and Persian sturgeon (Acipenser persicus) a reciprocal crosses with three treatments and three replicates for each treatment was conducted. Reproduction normative including number of eggs per gram, fertilization rate, survival rate, and also 32 mophmetric and merestic parameters of parents, hybrids and control groups were compared. Genetic analysis of hybrid was conducted using two methods of cytogenetic (chromosome preparation) and molecular (microsatellite) techniques. Histological analysis was performed for sexual gonad development. The growth comparison between hybrids and control fish was conducted in fiberglass tanks for 18 months. Fish were fed using pellets and biometric measurements were carried out 17 times during the study period. Means, analysis of variance, standard deviation, Duncan s test and percentage of hetrosis were calculated using Quatro Pro and SPSS programs. Significant differences were detected between beluga controls and hybrids (male beluga x female A. persicus) and between A. persicus controls and hybrids (male beluga x female A. persicus) regarding number of eggs per gram (P 0.003). However no significant differences were detected between the control groups and hybrids regarding fertilization rate at the four celled and 35 celled stages, number of larvae produced, mortality rate up to the onset of exogenous feeding and the number of larvae surviving (P〈0.01). Growth rates differed in hybrid fish and fish in the control groups and highest weight increase at the end of the rearing period belonged to beluga control (975±10 g) followed by hybrids produced by crossing female beluga with male A. persicus (840±143 g), hybrids produced by crossing female A. persicus with male beluga (681.85±281 g) and lowest growth increases belonged to the A. persicus control group (535.15±131 g). Specific growth rate in the second and third six months of rearing in hybrids produced by crossing female beluga with male A. persicus was higher than those recorded in the beluga control group. Percentage of hetrosis was negative during the early rearing period (-18.93), however at the end of the rearing period offspring were superior to parents and percentage of hetrosis was 0.79. Comparison of 32 morphologic and merestic parameters showed significant differences between 23 parameters between beluga controls and hybrids and between 31 parameters between A. persicus controls and hybrids (P,0.05). The hybrids production was proved using the cytogenetic (chromosomal count) as well as microsatellite techniques. The number of chromosomes in hybrids was intermediate to the parents (2n=190 ± 9) and like all other sturgeon species, microchromosomes comprised more than 50% of the chromosomes. The chromosome number in hybrids was half the number of chromosomes in the parents (A. persicus 2n=258±4 and beluga 2n=118±3). With regard to the fact that the number of chromosomes in A. persicus is 4N and that in beluga is 2N the number of chromosomes in hybrids is 3N or triploid. DNA bands produced by PCR in parents and offspring showed genetic inheritance. Histological analysis of control fish and hybrids after 18 months of rearing showed that male and female cells were observed in hybrids that is a characteristic feature of impotent or sterile fish. However only one type of sexual cells were observed in fishes in the control groups (A. persicus and beluga). Results obtained from the present study show that the hybrids produced are triploid (3N) and histologically sterile. Also hybrids produced showed good growth. With regard to the scarcity of female beluga and the limitation in the production of beluga fingerlings, it is suggested that sturgeon hatcheries produce hybrids and thus meet the fingerling demands of sturgeon farms. Also considering that the hybrids produced are sterile they can be considered as a candidate for export for aquarium fish. With regard to the fact that the hybrids produced are a new species it is suggested that this species is named Belupars which is a taken from the names of the two parents Beluga and Persicus.