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  • 1
    Publication Date: 2021-05-19
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 138pp.
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  • 2
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25792 | 18721 | 2018-10-13 08:54:26 | 25792 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Cheshmehkileh River and adjacent mountainous streams, play a strategic role as a historical axis for anthropogenic civilization, human welfare also habitat and migration pathway of commercial – biologic valuable fishes e.g. Caspian trout, Caspian kuttum, members of Cyprinidae family in south Caspian Sea drainage. Treats such as overfishing of Caspian trout and Red spotted trout stocks in mountainous headwaters, barriers construction and manipulations those are out of river carrying capacity developed by human activities, affected normal function of river as well. Sand mining big factories establishment next to the river, legal and illegal trade of river sediments, direct entry of Tonekabon landfill leakage into the river, development of Rainbow trout farms since 3 decades and huge effluents into the river containing dead fish and types of solids, escapement of cultured Rainbow trouts into the river, … are major minimum factors which needs basic information for integrating inclusively drainage management system. Cheshmehkileh River contains Headwaters of Dohezar (Daryasar & Nusha), Sehezar and Valamroud rivers during 13 monthly sampling phases between September 2009 and October 2010 based on macrozoobenthoses investigations by EPT, EPT/C EPA protocols, measurements of nominated physic-chemical and microbiologic parameters. Probability of Rainbow trouts escapement and invasion, existence, nutrition in Cheshmehkileh environment indeed investigated. Data analysis explained significant differences (P〈0.05) between groups of measured parameters in different sampling stations. Dendogram of clustered analysis based on consolidation of major biologic/ physic-chemical and microbiologic parameters, separated stations No. 1, 3, 2, 4 in one group and remained classified in different groups. Station 8 and 9 similarly separated which expressed general similarities according to Sehezar river environment which were differs in comparison with other stations. Station 11 separated according to its natural quality of water and environment. Similarities between station 10 to Sehezar river stations 8 and 9 expressed general influence of Sehezar River more than Dohezar River in Cheshmehkileh condition especially in station No. 10. High scores of EPT and EPT/C indices in upstream stations 1, 3 and 8 also low score of indices in stations 7, 13 and 6 expressed levels of environment quality between these groups of stations. Maximum average biomass of macroinvertebrates belongs to Trichoptera order in Cheshmehkileh River. Significant decrease of biomass in stations 11, 12 and 13 in comparison with other stations stated environment degradation in mentioned stations relevant to excessive sand mining as well. Pollution resistant groups of invertebrates significantly increased in downstreams against upstream stations. Also disappearing of Plecoptera order in station No. 7, 9, 10 and 13 stated low quality of environment in comparison with upstream stations. Confirmation of effects quality and quantity for point and non-point sources of imported pollutants require specific management considerations in order to present exploitations, pollutants control and emergencies for river monitoring in forthcoming years.
    Keywords: Aquaculture ; Management ; Iran ; Tonekabon ; Cheshmehkileh ; River ; Pollution ; Aquaculture ; EPTC ; Assessment ; Macroinvertebrates ; Chemistry ; Microbiology
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 138
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  • 3
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25642 | 18721 | 2018-10-14 02:40:17 | 25642 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol(7), based on their spectral data and from comparison with those previously reported in the literature.
    Keywords: Biology ; Chemistry ; Iran ; Oman Sea ; Persian Gulf ; Brown Algae ; Padina boergesenii ; Steroids compounds ; Extraction ; Purification ; Identification ; Amount verification ; Sargassum glaucescens ; Algae ; Abundant ; Sampling ; Chromatography ; Cephadex ; Dehydrocholesterol ; Cholesterol ; Fucosterol ; β-sitosterol ; Stigmasterol ; Ostreasterol
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 42
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  • 4
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25179 | 18721 | 2018-09-03 17:23:17 | 25179 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: In this study two species of algae, Sargassum glaucescens and Padina boergeseni that found plenteously in Persian Gulf and Bushehr coast, were collected and hot water extracts of them were lyophilized. F. indicus (11.32±1.20 g), after two weeks adaptation in Shoghab research station were immersed in seawater (39 ppt and 25±1 ○C) containing hot-water extract of each brown algae, S.glaucescens and P.boergeseni, at 100, 300 and 500 mg/l concentration, Survival rate and immunological parameters (total haemocyte count (THC), total plasma protein (TPP), Phagocytic activity, bacterial clearance efficiency and bactericidal activity) were examined. In addition effect of dietary administration of beta 1, 3 1, 6 glucan on prevention of White Spot Disease and immunological parameters of shrimp were investigated. According to results, immersion in seawater containing 300 and 500 mg/l concentration of algal hot-water extract after 2 and 3 hours or oral administration of beta 1,3 1,6 glucan at level of 10 g/kg diet for 14 days significantly enhanced THC, TPP, Phagocytic activity, bacterial clearance efficiency and bactericidal activity. Immersion in seawater containing 100, 300 and 500 mg/l hot-water extract of S.glaucescens after 3 hours, improved the survival rate of WSSV-infected F. indicus.
    Keywords: Biology ; Chemistry ; Iran ; Persian Gulf ; Bushehr Province ; Beta-glucan ; Sargassum ; Padina algae ; WSD ; Fenneropenaeus indicus ; Species ; Algae ; Brown Algae ; S.glaucescens ; P.boergeseni ; Survival rate ; White Spot Disease ; Shrimp
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 57
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  • 5
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25772 | 18721 | 2018-10-13 08:08:05 | 25772 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Seaweed belonged to baseborn marine plants with cell wall containing of valued polysaccharides such as Agar, Alginate, Limen sulfate and Carmine, used as food additives and in different industries. Base on previous studies, more than 1000 MT. of Sargassum seaweed withdraw from Oman Sea in Sistan and Baluchistan coastal line which can be collect, dried and powdered for using as supplement in shrimp feed. In this project, Sargassum ilicifolium collected from 6 coastal areas, rinsed, dried, powdered and measured the nutritional values in laboratory for surveying statistically. According to the high nutritional value of Tis coastal seaweed, this variety seaweed powder, replaced with protein resources (fish meal and Soy and Wheat) of white-leg shrimp feed which was formulated by Havorash feed factory of Boshehr in four treatments (A: as control without any replacement) B: with 5%, C: 10 % and D: 15% seaweed replacement, each with three replicates in order to obtain isonitrogenus 33% CP., and iso-caloric (13% fat and 15% carbohydrate) feed using winfeed software. The weighed milled ingredients were carefully mixed using a laboratory food mixer. The mixtures were primed with 30% hot water to yield a suitable pulp. Wet diets were made into 2 mm pellet size and dried at 40 °C in a drying cabinet and maintained in standard condition which was used for water stability and absorption capacity test of the pellets in sea water, statistically one way- ANOVA. The Tis coastal seaweed with 9.8% CP, 2% lipid and 23% carbohydrate had higher nutritional value compared to the other gathered seaweed. Also amino acid and fatty acid profiles, vitamins and minerals were measured in all seaweed samples each, with three replications. As result, for using Sargassum ilicifolium as vitamins and mineral premixes in white- leg shrimp feed, Zinc, Cobalt and Phenylalanine with dose of 1.1, 06.0 and 4.0 ml. 100% dried seaweed must be added respectively. The water stability of D feed treatment in seawater (98%) and C (97%) had statistical differences with A and B (95% stability) (P〈0.05). Water absorption capacity of feeds after one hour immersion in seawater showed significance difference between D (110%) and three others, C(100%), B(85% and control(80%) (P〈0.05). As final aim of this project, enriched product of this seaweed as minerals and vitamins supplements were handsel joinery in the Second Medicine Plant Festival, 2016 and registered in recording organization to give the final certificate.
    Keywords: Aquaculture ; Chemistry ; Iran ; Sargassum illicifolium ; Sargassum illicifolium ; Mineral and vitamins supplements ; Natural binder ; Litopenaeus vannamei ; Seaweed ; Vitamin ; White leg shrimp
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 82
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  • 6
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25713 | 18721 | 2018-10-10 10:11:15 | 25713 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Chitin nanofibers are prepared from the exoskeletons of shrimp by a simple mechanical treatment after a series of purification steps. The nanofibers have fine nanofibers networks with a uniform width of approximately 10 nm. Grinders and highpressure water jet systems are effective for disintegrating chitin into nanofibers. Acidic conditions are the key factor to facilitate mechanical fibrillation. Ultrafine fibers were successfully fabricated chitosan and fish skinextracted gelatin via electrospinning (ES). Important ES parameters, such as concentration of aqueous acid and fish gelatin solutions, and electric field intensity were examined to investigate the effects on the morphology of the gelatin nanofibers. Due to the poor mechanical properties of the fish gelatin membranes, composite nanofibers made of fish gelatin and poly(L-lactide)(PLLA) were produced with a novel solution. The introduction of PLLA remarkably improved the mechanical properties of the gelatin membranes. With a combination of good biocompatibility and mechanical properties, fish gelatin/PLGA blending non-woven mats are considered to be very promising in fish fillet coating application.. in this study, we fabricated a novel nanofibers composed of fish collagen (FC) and polycaprolactone (PCL) blends by using the electrospinning method. Nanofibers were characterized using a scanning electron microscope (SEM), and it was revealed that the diameter of nanofibers decreases as FC content was increased in the FC/PCL composite nanofibers. Several modifications to the chitin NF surface are achieved, including acetylation, deacetylation and maleylation. The results of this study revealed that: 1–It is possible to produce Nanofibers from chitosan and fish gelatin. 2– Covering and coating of processed fish by nanofibers are applicable and increasing the possibility of shell life for the processed fish. 3– Nanofibers which have been produced from chitosan and fish gelation not only is environmentally friendly but also it will be eatable while has been covered for fish fillets. biocompatible chitosan and gelatin made from fish, fresh fish fillets do not have the ability to cover and packaging, but is edible and used.
    Keywords: Biology ; Chemistry ; Fisheries ; Iran ; Chitin ; Chitosan ; Nanofiber ; Chemical modification ; Fish ; Aquatic ; Maintenance
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 66
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  • 7
    Publication Date: 2021-07-16
    Description: This study was conducted to determine phosphorous and nitrogen species at the surface sediment in the southern Caspian Sea (Mazandaran Coast, Kelarabad). Surface sediments samples were collected at three stations from autumn of 2011 to winter of 2012. All samples were prepared by digesting and extracting processes and then phosphorous and nitrogen species concentrations determined using spectroscopy instrument. Results of this study showed that annual mean of Loosely-P, Fe-P, Al-P, Bioava.-P, Ca-P, TIP, Rese.-P and TP was obtained as 5.06±0.33, 55.11±2.52, 42.38±3.74, 102.52±5.68, 172.91±7.12, 275±9.12, 333.30±28.52 and 608±52 µg/g.dw, respectively. The results also showed that inorganic phophorous was less than organic phophorous during different seasons, however, mean percentage of residue-P containing organic compounds and non-degradable compounds was more than 50 percent. Percentage of Ca-P was higher than 60, whereas Bioava.-P was less than 40 percent. In addition, Fe-P and Loosely-P attained the maximum and minimum values, respectively, among the bioavailable phophorous. The order of different forms of phosphorous were recorded as Org-P〉Ca-P〉FeP〉Al-P〉Loosely-P. Annual mean of NH4/N, NO2/N, NO3/N, TIN, TON and TN were observed as 4.23±0.50, 0.06±0.01, 0.74±0.12, 5.02±0.53, 2.48±0.63 and 7.53±0.51 µg/g.dw, respectively. Annual percentage of TIN was two folds than TON and concentration of NH4/N was also four times than NO3/N. As a conclusion, the results revealed that main causes of Bioava.-P adsorption and desorption were temperature, Eh and pH. Also, the form of NH4+/N was of a high percentage because of anaerobic condition in the sediments. According to the high ratios of nitrogen/phosphorous of sediments to nitrogen and phosphorous of bottom water, it finds that released of those from the sediments to water will be happened with high rates. Therefore, it is expected that the establishment of fish farming cages should be carried out with more precautionary approaches which not leads to increased algae bloom.
    Keywords: Biology ; Chemistry ; Ecology ; Iran ; Caspian Sea ; Kelarabad ; Mazandaran coast ; Phophorous ; Nitrogen ; Surface sediment ; Determination ; Breeding
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 42
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  • 8
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25580 | 18721 | 2018-10-07 10:35:48 | 25580 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: By-products consist of more than half of the total weight of fish. Most part of these wastes made from connective tissue proteins that provide good potential to produce gelatin. Nowadays, consumers are more health conscious and have responded to the call for a diet that contains low calorie and low fat. This work involved the characterization of a low-fat spread made of fish gelatin as gelling agent, emulsifier and fat replacer. Six treatments with Fish gelatin (FG) to pectin ratio 1:1 and 2:1, with pectin concentration of 1, 2 and 3%, were produced and evaluated for quality changes and storage-life at refrigerated storage. The results showed that prepared emulsions were physically stable and no phase separation observed during 8 wks. The pH of treatments was 4.5 to 4.9. The moisture content of treatments varied from 68.9% for T1 (FG/pectin ratio 1:1) to 65.4% for T6 (FG/pectin ratio 6:3). The Max. lipid of samples was measured 28.4%. Max. and min. protein of samples were 3.8% for T6 and 1.6% for T2 (FG/pectin ratio 2:1), respectively. No significant differences (p≥0.05) were observed between ash content of all treatments and control (commercial margarin). The acidity of treatments increased during storage and were significantly different from production day (p≤0.05). Peroxide value (PV) of treatments increased significantly at refrigerated storage. No significant differences were observed between PV of treatments during different wks of sampling. The results of texture profile analysis showed that firmness, compressibility and adhesiveness properties were significantly increase with higher substitution of FG with pectin. Color, Aroma, Taste and texture properties of prepared samples were found to significantly lower than control, according to sensory evaluation. Significant increases were observed in mold/yeast and psychrophilic count of treatments during storage. The macroscopic growth of molds was observed on all samples in 8th wks. No coliform growth was observed in all treatments at any time. Results suggest that low-fat spread properties were significantly influenced by different ratios of fish gelatin and pectin incorporated. It seems that these results can provide new opportunities to develop market with introducing novel products to response consumer demands. Some quality defects observed in this study can be improved by using suitable machinery at industrial scale.
    Keywords: Biology ; Chemistry ; Iran ; Fish gelatin ; Low-fat spread ; Fish wastes ; Quality characterictics ; Texture profile ; Shelf-life ; Refrigerated temperature ; Cultured Carp ; Diet ; Lipid
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 74
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