Publication Date:
2004-03-20
Description:
Chromosome substitution strains (CSSs) have been proposed as a simple and powerful way to identify quantitative trait loci (QTLs) affecting developmental, physiological, and behavioral processes. Here, we report the construction of a complete CSS panel for a vertebrate species. The CSS panel consists of 22 mouse strains, each of which carries a single chromosome substituted from a donor strain (A/J) onto a common host background (C57BL/6J). A survey of 53 traits revealed evidence for 150 QTLs affecting serum levels of sterols and amino acids, diet-induced obesity, and anxiety. These results demonstrate that CSSs greatly facilitate the detection and identification of genes that control the wide diversity of naturally occurring phenotypic variation in the A/J and C57BL/6J inbred strains.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Singer, Jonathan B -- Hill, Annie E -- Burrage, Lindsay C -- Olszens, Keith R -- Song, Junghan -- Justice, Monica -- O'Brien, William E -- Conti, David V -- Witte, John S -- Lander, Eric S -- Nadeau, Joseph H -- GM07250/GM/NIGMS NIH HHS/ -- HD07518/HD/NICHD NIH HHS/ -- RR12305/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2004 Apr 16;304(5669):445-8. Epub 2004 Mar 18.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Broad Institute, Cambridge, MA 02142, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15031436" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acids/blood
;
Animals
;
Anxiety/genetics
;
*Chromosome Mapping
;
Chromosomes, Mammalian/*genetics
;
Crosses, Genetic
;
Diet
;
Female
;
Genetic Predisposition to Disease
;
Genetic Variation
;
Male
;
Mice
;
Mice, Inbred A
;
Mice, Inbred C57BL
;
Microsatellite Repeats
;
Obesity/genetics/physiopathology
;
Phenotype
;
*Quantitative Trait Loci
;
*Quantitative Trait, Heritable
;
Sterols/blood
;
Weight Gain
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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