Publication Date:
2015-11-21
Description:
Genetically encoded voltage indicators (GEVIs) are a promising technology for fluorescence readout of millisecond-scale neuronal dynamics. Previous GEVIs had insufficient signaling speed and dynamic range to resolve action potentials in live animals. We coupled fast voltage-sensing domains from a rhodopsin protein to bright fluorophores through resonance energy transfer. The resulting GEVIs are sufficiently bright and fast to report neuronal action potentials and membrane voltage dynamics in awake mice and flies, resolving fast spike trains with 0.2-millisecond timing precision at spike detection error rates orders of magnitude better than previous GEVIs. In vivo imaging revealed sensory-evoked responses, including somatic spiking, dendritic dynamics, and intracellular voltage propagation. These results empower in vivo optical studies of neuronal electrophysiology and coding and motivate further advancements in high-speed microscopy.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gong, Yiyang -- Huang, Cheng -- Li, Jin Zhong -- Grewe, Benjamin F -- Zhang, Yanping -- Eismann, Stephan -- Schnitzer, Mark J -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2015 Dec 11;350(6266):1361-6. doi: 10.1126/science.aab0810. Epub 2015 Nov 19.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉James H. Clark Center, Stanford University, Stanford, CA 94305, USA. CNC Program, Stanford University, Stanford, CA 94305, USA. Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA. yiyang.gong@duke.edu mschnitz@stanford.edu. ; James H. Clark Center, Stanford University, Stanford, CA 94305, USA. ; James H. Clark Center, Stanford University, Stanford, CA 94305, USA. CNC Program, Stanford University, Stanford, CA 94305, USA. ; James H. Clark Center, Stanford University, Stanford, CA 94305, USA. CNC Program, Stanford University, Stanford, CA 94305, USA. Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA. ; James H. Clark Center, Stanford University, Stanford, CA 94305, USA. CNC Program, Stanford University, Stanford, CA 94305, USA. Howard Hughes Medical Institute, Stanford University, Stanford, CA, USA. yiyang.gong@duke.edu mschnitz@stanford.edu.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26586188" target="_blank"〉PubMed〈/a〉
Keywords:
*Action Potentials
;
Animals
;
*Bioluminescence Resonance Energy Transfer Techniques
;
*Biosensing Techniques
;
Dendrites/physiology
;
Drosophila melanogaster/physiology
;
*Evoked Potentials, Somatosensory
;
*Fluorescence Resonance Energy Transfer
;
Green Fluorescent Proteins/chemistry/genetics
;
Mice
;
Neurons/*physiology
;
Recombinant Fusion Proteins/chemistry/genetics
;
Rhodopsin/chemistry/genetics
;
Smell
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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