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  • Huso huso  (14)
  • Management
  • Microsatellite
  • 2020-2022  (20)
Collection
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Years
Year
  • 1
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    In:  http://aquaticcommons.org/id/eprint/21944 | 18721 | 2018-01-18 08:55:31 | 21944 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-05
    Description: A total of 120 samples of adult common Kilka fish (Clupeonella cultriventris) were collected during spring and summer from the southern coasts of Caspian Sea (Bandar Anzali and Babolsar). Fifteen sets of microsatellite primers were developed from Clupeidae being tested on genomic DNA of common Kilka. Allele frequency, observed and expected heterozygosity, FST, RST, FIS index were determined. Five primer sets as polymorphic loci were used to analyze the genetic variation in adults of the common Kilka population. Results revealed that average alleles per locus was 13.1 (range 5 to 22 alleles per locus in regions, Ne=9.5). All sampled regions contained private alleles. Average observed and expected heterozygosity was 0.348 and 0.877, respectively. Deviations from Hardy Weinberg equilibrium were observed in most cases. FST, RST and gene flow estimates in AMOVA and the genetic distance between populations indicated that the genetic difference among the studied populations was pronounced. The data generated in this study provide primary information on the genetic variation and differentiation in populations of Caspian common Kilka.
    Keywords: Biology ; Fisheries ; Population ; genetic ; Microsatellite ; Clupeidae ; Common kilka ; Clupeonella cultriventris ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 139-148
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  • 2
    Publication Date: 2021-07-06
    Description: The objective of this study was to analyse the population genetic structure of the Persian sturgeon (Acipenser persicus) in Sefidrud and Gorganrud rivers watershed based on the characterization of microsatellite markers during 2006 - 2008. 100 samples of Persian sturgeon were collected from two regions. Four microsatellite loci (Ls68, Spl168, Spl173 and Afu68) were analyzed for the molecular characterization of this species which resulted in polymorphic patterns. DNA bands were analysed using Biocapt and GenAlex software package. A total of 109 alleles were observed of which the maximum number of alleles (17) were found in Spl168 locus which belonged to sturgeons from Sefidrud river's watershed and the minimum number of alleles (10) in Ls68 locus belonging to the sturgeons from Gorganrud river's watershed. Results of microsatellite analysis revealed that the differences between samples of two regions were not statistically significant (p〉0.05), neither for the average number of alleles per locus nor for observed heterozygosities. The calculated Fst and Rst between two regions was 0.07 and 0.17 showing that the genetic difference was significant (p〈 0.01). Samples from Sefidrud river's watershed in Spl173, Afu68 and Spl168 loci and samples of other regions in Afu68 and Spl168 loci were at Hardy-Weinberg equation. The genetic distance was calculated as 0.4 which represents a significant genetic difference between samples of two studied areas. In conclusion, this study suggests that the Persian sturgeons in two regions of the southern part of the Caspian Sea are genetically differentiated, therefore fisheries management of these unique stocks for restocking and conservation of gene pools is highly recommended.
    Description: Article includes abstract in Farsi on last page.
    Keywords: Biology ; Fisheries ; Persian sturgeon ; Acipenser persicus ; Caspian Sea ; Microsatellite ; Genetic structure ; Population genetic ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 596-606
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  • 3
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    In:  http://aquaticcommons.org/id/eprint/23736 | 18721 | 2018-07-21 17:18:02 | 23736 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-15
    Description: Six diets supplemented with 0, 100, 200, 400, 800, and 1600mg/kg L-Ascorbyl-2-Polyphosphate (APP) as vitamin C, were fed to juvenile beluga sturgeon in three replicates for 16 weeks. Fifty five fish with an average weight 38.1 plus or minus 0.5 g were distributed to each tank after adaptation to the experimental diets. Most of the growth performance indices at week 4 indicated significant differences (P〈0.05), except in CF and PER (P〈0.05). The survival was affected by dietary treatment at week 4 (P〈0.05). No deficiency sign was observed during the trial. Results of this study indicated that vitamin C can have remarkable effects on growth indices in different growth periods. Therefore, regarding the effects of vitamin C on the growth indices and in the fish weight range used in this study, 100-200 mg/kg vitamin C (APP) can be considered as the optimum level for rearing the fish.
    Keywords: Aquaculture ; Growth rate ; Vitamin C ; Feeding ; Juveniles ; Condition factor ; Huso huso ; Brackish ; Caspian Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 107-120
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  • 4
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25651 | 18721 | 2018-10-14 02:48:20 | 25651 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This study was conducted to codify the guideline and indexes of broodstock management in salmonid fish and cryopreservation, selective breeding and sex reversal in hatchery to attain responsible aquaculture development. Cryopreservation includes of sperm and egg in term of economic is important in aquaculture. In this report mentioned the type and name of material. All female and sex reversal fish are produced by steroid hormone which mentioned in this report. Selective breeding include individual selection and family selection which could be used for genetic improvement of fish.
    Keywords: Aquaculture ; Biology ; Management ; Iran ; Broodstock ; Reproduction ; Cryopreservation ; Genetic ; Breeding ; Aquaculture ; Salmonid fish ; Sex reversal ; Hatchery ; Sperm ; Egg ; Female ; Steroid hormones
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 46
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  • 5
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    In:  http://aquaticcommons.org/id/eprint/22103 | 18721 | 2018-02-08 03:27:52 | 22103 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-06-26
    Description: A feeding trial was conducted to examine the effects of dietary supplementation of lysine and methionine on growth, nutrient utilization and carcass compositions in beluga, Huso huso. Four experimental soy protein based diets D0 (without lysine or methionine supplementation), D1 (lysine supplementation alone), D2 (methionine supplementation alone) and D3 (both lysine and methionine supplementation) were fed to triplicate groups. 240 fish with an average weight of 144.6 ± 4.64 g were fed the experimental diets for 12wk. L-Lysine and DL-methionine were added to the diets containing 344 g kg-1 soybean meals at 22.02 and 12.94 g kg-1 of dry diet respectively. Significant higher weight gain, specific growth rate, protein efficiency ratio, condition factor, feed efficiency ratio, whole muscle protein and protein apparent digestibility were observed in fish fed soya protein-based diet supplemented with both lysine and methionine than that of fish of other dietary groups. There were no significant differences among fish fed diet supplemented with either methionine or lysine. Growth performances, feed utilization, carcass composition and apparent digestibility coefficients of protein, lipid and dry matter of fish fed control diet was significantly lower than those of fish fed diet supplemented both lysine and methionine and supplemented with either methionine or lysine. It can be concluded that beluga, fingerlings effectively utilize the supplemental amino acids (L-Lysine at 22.02 g kg-1 dry diet and DL-methionine at 12.94 g kg-1 dry diet) in the diets containing 344 g kg-1 soybean meals for better carcass proximate compositions, growth performance and feed efficiency and utilization.
    Keywords: Aquaculture ; Biology ; Chemistry ; Soy protein ; Nutrient utilization ; Apparent digestibility coefficients ; L-Lysine ; DLmethionine ; Beluga ; Huso huso ; diet ; growth ; compositions ; lysine ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 119-134
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  • 6
    Publication Date: 2021-07-07
    Description: In order to have a sustainable management on Persian sturgeon as a highly commercial species in the South Caspian Sea, we need to identify its population structure and the level as well as its conservation status in their natural habitat. To develop a conservation program for this all Caspian Sea' sturgeon species it requires knowledge of its genetic diversity using reliable molecular marker to study population genetic structure. For these purposes, an enriched library was prepared based on a modified biotin-capture method. Approximately 1800 positive clones were screened for microsatellites in an Acipenser persicus genomic library. Of these 350 positively hybridizing clones were sequenced, and 81 clones were identified as having microsatellites with adequate flanking regions. We developed and tested 68 microsatellite primer pairs for Persian sturgeon. Out of 68 primer pairs developed, 11 pairs resulted in poor or no amplification, 13 were ambiguous, 6 were monomorphic, 20 were tetrasomic and 18 were octosomic in Persian sturgeon. While none of the markers showed disomic inheritance in Persian sturgeon and Russian sturgeon (A. gueldenstaedtii). Several of the markers appeared useful for studies stellate sturgeon (A. stellatus), ship sturgeon (A. nudiventris) and beluga (Huso huso). Nearly all the polymorphic pattern for ship, stellate and beluga displayed the simple banding patterns characteristic of disomic loci, while those for Russian sturgeon displayed banding patterns characteristic of tetraploid or higher polyploid levels. These markers may prove useful in a variety of future sturgeon population genetic studies in the Caspian Sea.
    Keywords: Biology ; Fisheries ; Persian sturgeon ; Acipenser persicus ; Caspian Sea ; Microsatellite ; Population genetic ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 548-558
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  • 7
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    In:  http://aquaticcommons.org/id/eprint/22321 | 18721 | 2018-03-21 02:12:20 | 22321 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-03
    Description: The effect of feed supplemented with L-carnitine at four levels of 0, 300, 600 and 900mg kg-1 on growth performance and body composition of beluga, H. huso, juveniles were evaluated in two separate trials. In the first trial, 180 juveniles (525±9gw) were fed with the test diet for a period of 71 days, and the trial extended to another 126 days in the second phase by randomly selecting 120 juveniles (870.7±32g) and rearing them with similar diets. All trials, in triplicates, were conducted in circular fiberglass tanks (200cm diameter, 40cm height).The fish were fed 4 times daily to apparent satiation. In the first phase, L-carnitine did not significantly affect mean final weight, feed conversion ratio (FCR) or specific growth rate (SGR) of the fish, however, the fish receiving 900mg kg-1 L-carnation showed better growth increment, SGR and feed efficiency (FE) than those in control group (p³0.05); neither did it significantly affect protein, lipid, moisture or ash as well as leptosomatic index (HSI) (p³0.05). The protein efficiency ratio (PER) was significantly affected by L-carnitine supplemented diet, particularly at 900mg kg-1(p£0.05). In the second phase, fish fed L-carnitine-supplemented diets produced significantly higher W2, SGR and PER than the control group (p£0.05), but HSI decreased significantly (P£0.05). At the end of the trial, the whole body composition of fish did not differ significantly among experimental treatments, but slightly reduced lipid content was observed at 600 and 900mg kg-1 L-carnitine (p£0.05).The results of this study indicated that supplementation of diets with 300-600mg carnitine kg-1 improved growth rate, feed utilization and stimulated protein-sparing effect in this species.
    Keywords: Biology ; Fisheries ; L-carnitine ; Growth rate ; Body composition ; Beluga ; Huso huso ; iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 157-170
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  • 8
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    In:  http://aquaticcommons.org/id/eprint/22336 | 18721 | 2018-03-22 17:51:18 | 22336 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-03
    Description: Population genetic structure of stellate sturgeon (Acipenser stellatus) in 197 adult specimens from four fishery regions along the Iranian coastline of the southern Caspian Sea was investigated using microsatellite markers. Out of 15 microsatellite primers, 11 loci were produced, in which 10 of them were polymorphic and 1 was monomorph. Totally, 184 alleles were identified and on average 13.1 alleles per locus were found (ranged 8 to 18 alleles). All sampled regions contained unique alleles. Average observed and expected heterozygosity was 0.667 and 0.854, respectively and significant genetic differences between 4 regions were observed (p≤0.01). Deviations from Hardy-Weinberg equilibrium were found in most cases. Population differentiation test was modest and significant (p≤0.01). Based on FST estimate (p≤0.01), more than one population of stellate sturgeon is identified in the south Caspian Sea. Therefore, fishery management for restocking and conservation of gene pool is highly recommended.
    Keywords: Biology ; Fisheries ; Stellate sturgeon ; Population genetics ; Microsatellite ; South Caspian Sea ; Iran ; Acipenser stellatus
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 73-84
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  • 9
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    In:  http://aquaticcommons.org/id/eprint/24686 | 18721 | 2018-08-04 15:58:43 | 24686 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-15
    Description: A two-phase random growth test was conducted to evaluate the effects of feeding rate on growth performance, food conversion ratio (FCR), specific growth rate (SGR), feeding efficiency and price index of reared Huso huso fingerlings in fiberglass tanks (2x2x0.53 cm). Rearing conditions such as diet, dissolved oxygen, light, water flow, fish density and feeding frequency were kept similar for the tanks. In the first phase, 180 fingerlings with a mean weight 867.86±17.42g were fed for 100 days in four treatments and three replications at feeding rates 1, 2, 3 and 4 percent of their biomass. In the second phase, also four treatments and three replications were applied to 84 Huso huso with a mean weight 2096.1±35.6g. In this phase, the fish were fed for 125 days with 0.75, I .5, 2.5 and 3 percent of their biomass. During rearing period, water temperature was 25.52±1.78 and 14.82±0.48 degrees centigrade and dissolved oxygen was 7.6±0.3 and 7.89±0.18 mg/lit, for the two phases respectively. The fishes were fed four times daily with a diet containing 40% protein, 13% fat and 9.9% ash. All treatments induced fast growth in the fish, but feeding ratios were different in their effects on the growth (P〈0.05). Weight gain percentage, specific growth rate (SGR), food conversion ratio (FCR), feeding efficiency and price index indicated that lower feeding ratios were more effective in causing fish growth (P〈0.05). So, increase in feeding ratio directly increased daily food consumption (D.F.C) and negatively affected the feeding efficiency, food conversion ratio, specific growth rate and price index (P〈0.05). The results showed that in phase one, when the fish were given food as much as two percent of their body weight, one unit of meat was produced by consuming 1.92 units of food. In the second phase, feeding fish with 0.75 percent of their body weight resulted in producing one unit of fish meat per 1.82 units of food consumed. Also, in the two phases, application of higher than 50% feeding ratio increased costs while no significant trend in fish growth was detected. It can be concluded that regarding the water temperature, the optimal feeding rate for fishes weighing 850 to 1900 grams and those weighing 2050 to 3300 grams are 2 % and 1 % of body weight, respectively.
    Keywords: Aquaculture ; Huso huso ; Food Conversion Ratio ; Growth Trend ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 165-180
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  • 10
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25645 | 18721 | 2018-10-14 02:39:41 | 25645 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: The lack of sustainable management programs has resulted in the drastic decline of sturgeons stocks in the Caspian Sea in the past two decades. Legal catch quotas for all Caspian littoral states has dropped from 28500 tons in 1985 to 460 tons in 2007 while caviar production in the Caspian Sea during the same period decreased from 3000 tons to 70 tons. Caviar production in the I.R. of Iran dropped from 305 tons in 1985 to about 11 tons in 2007. The "Strategic and applied research planning for sturgeon management and conservation" was developed with the collaboration of academicians from universities, researchers, experts, fishery authorities and representatives of the executive government to maintain sustainable development and rational management of sturgeon stocks in the Caspian Sea. The preparation and development of this planning was carried out through 121 sessions (2100 person hours) A comprehensive report (375 pg) was prepared which was divided into five volumes; Volume I comprises an introduction, objectives, strategies and planning, present status of production and caviar harvest, catch and export quotas, revenue generated and release of sturgeon fingerlings, Volume II comprises analysis of the problem by constructing a problem tree with 8 broad categories to analyze 344 problems and an objective tree which is the hierarchic flowchart of objectives with 9 broad categories with 241 items, Volume III comprises a review and analysis of previous and ongoing research (414 projects) on different aspects of sturgeon during the past 40 years, Volume IV comprises prioritizing research objectives outlined by the objective tree and finally Volume V which prioritizes primary objectives for 14 executive and 10 research programs. On the basis of the evaluation of the present status and for the sustainable development and rational management of sturgeon stocks a strategic and applied research planning program was proposed and developed within the framework of three primary objectives; 1) Management and sustainable use of Caspian Sea resources (5 programs), 2) Rehabilitation and restoration of stocks (5 programs), 3) Development of aquaculture (4 programs). The applied research program for each proposed executive program was outlined in 10 programs, 42 comprehensive plans and 222 projects. The primary objective on management and sustainable use of Caspian Sea resources includes 5 programs, 14 comprehensive plans and 63 projects, while the primary objective on rehabilitation and restoration of stocks includes 4 programs, 16 comprehensive plans and 87 projects. The primary objective to address sturgeon aquaculture includes 1 program, 12 comprehensive plans and 72 projects. The impacts and outcome of each of these programs was determined and presented. It is evident that if the present situation persists, the catch figures for adult sturgeon specimens and caviar production in Iran will reach zero in 2021. But if concerted efforts are taken and the proposed strategic and applied research planning program is executed (commencing from 2009) we can put a halt to these declining trends. By producing sturgeon fingerlings to restore population abundances, by conserving and protecting them in the Caspian Sea and by conducting applied research we can produce 206.4 tons of caviar by the year 2033. Apart from harvesting caviar from the Caspian Sea we can also annually produce 3000 tons sturgeon meat and 60 tons farmed caviar starting from the year 2023. The total budget proposed for implementation of this program for a period of 14 years (2008-2022) is USD 2483 million. If this budget is allocated in time and the proposed strategic program is properly and totally implemented, we can not only save many sturgeon populations from extinction but also ensure job opportunities for 6000 fisherman, provide 1480 new job opportunities and annually produce 266 tons of caviar which will generate USD 4957 million.
    Keywords: Management ; Iran ; Sturgeons ; Strategic Program ; Conservation
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 350
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