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  • Biotechnology
  • 2020-2022  (10)
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  • 1
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25716 | 18721 | 2018-10-14 03:15:28 | 25716 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Genetic knowledge helps to protect biodiversity and optimal harvest resources by several ways. This knowledge can help to reduce the risk of extinction to those populations that have high genetic diversity or diminished by detected them. Also, studies of population genetics can present guidelines for improving the structure of the population and understanding the biology of species. One of the most important applications of DNA database is detecting species, fishing offense, diagnose of anemia and genetic classification of animals. DNA bank has helped to identify the species that are hunted and were discovered only parts of their meat and texture. Optimal Storage of aquatic genetic resources and the conservation of biological diversity, as the national capital by using biotechnology methods are the most important goals of gene bank and database reserves of the country creation. Collecting biological specimens of aquatic species, preparation and long-term maintenance of them, genetic registration of endangered and threatened species and the use of biotechnology techniques for the protection, conservation and management of aquatic genetic resources is one of the other functions of the gene bank. Gene bank of inland waters of Iran (Gorgan) is planned and implemented to identify, collect, maintain and protect species at the level of genes, cells, tissues and live fish research and commercial exploitation. Despite the predicted projected funding for this project, unfortunately funding sources have been considered, does not allocated. Therefore some parts of the aims of this project has been organized by Golestan province credits to build molecular laboratory and aquaculture pond in Alang farm and exploitation of potential Gharasou station and Sijoval center restocking and the conditions for the bulk of the project goals is provided. Some parts like tissue culture laboratory and bioinformatics as well as parts and equipment related to the freezing of sperm and embryo development are the items that should be considered in the development of the this bank.
    Keywords: Biology ; Iran ; Inland Water ; GenBank ; Gene Bank ; Fish ; Species ; Genetic ; Biotechnology
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 74
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  • 2
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25503 | 18721 | 2018-10-05 07:54:25 | 25503 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Specialists in Marine biotechnology using marine biological engineering and scientific principles to develop natural products and regarded services. One of the most important applications of this new branch is produce the natural products such as enzymes, natural colors, vaccines and drugs and etc. which produced from marine living organisms. These products are used in various industries such as pharmaceutical, food or health industry. In the current study, we try to remark the application of marine biological products in the northern (Caspian Sea), southern (Persian Gulf and Oman Sea) or internal waters of Iran into six categories, including aquaculture, pharmaceutical industry, food, healthcare and cosmetic industries. For this purpose, the required information will be collected using investigations which conducted inside and also outside of our country by reviewing and analysis of derived data by attention to our economical facilities for national self-sufficiency in production. Applied research projects will be introduced in three parts including research, technology development and pilot plant as well. Hope that using this strategic document will be effective and practical to production of biological products from marine resources until 2025.
    Keywords: Management ; Iran ; Enzymes ; Marine ; Biological ; Biotechnology ; Aquatic
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 131
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  • 3
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    In:  http://aquaticcommons.org/id/eprint/22914 | 18721 | 2018-06-06 01:29:12 | 22914 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-11
    Description: Fatty acids composition in edible parts of blue swimming crab (Portunus pelagicus) caught in the Persian Gulf close to the Bushehr Province were investigated. Among saturated fatty acids (SFA), the 16:0 and 18:0 fatty acids were the most two dominant fatty acids in meat tissues that were analyzed. 18:1n-9 and 16:1n-7 were the major fatty acids among the MUFAs of male and female crab. The main PUFAs in lipids of crab meat were 20:5n-3 and 22:6n-3. The n-3 PUFA content was highest in female claw meat (20.26%) and lowest in male meat (11.31%), respectively. The sex and tissue had a significant influence on the EPA, DHA and n-3 HUFA. The highest amounts of DHA and the favorable n-6/n-3 ratio in the meat of blue swimming crab indicate that the claw and breast meat is a very omega-rich edible portion of crab body.
    Keywords: Biology ; Fisheries ; Portunus pelagicus ; Fatty acids ; Tissue ; Sex ; Persian Gulf ; Biotechnology ; Bushehr Province ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 818-826
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  • 4
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    In:  http://aquaticcommons.org/id/eprint/22898 | 18721 | 2018-06-05 22:30:52 | 22898 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-11
    Description: The application of new probiotics is a good strategy in the biological control of infectious diseases in aquaculture. Approximately 100 marine actinobacteria isolates were obtained from 10 sediment samples of shrimp farms. Heat treatment of sediment samples resulted in a selective reduction of the non actinobacterial heterotrophic microflora. Starch nitrate agar medium exhibited more efficacy than glycerol arginine agar medium for isolation. Twenty seven percent of actinobacterial isolates showed antagonistic activities against pathogenic Vibrio spp. All the antagonistic isolates showed the typical morphology of genus Streptomyces. Exoenzymatic activity screening showed that 44%, 26%, 37% of antagonistic isolates represented amylase, lipase and protease activities, respectively. MNM-1400 strain exhibited highest antagonistic and exoenzymatic activity. The pathogenicity experiment revealed that MNM-1400 strain did not cause disease in Litopenaeus vannamei larvae. Extraction of produced antibacterial compounds by MNM-1400 strain showed that the active constituent didn’t have non polar property. Morphological, physiological and biochemical identification confirmed that MNM-1400 strain belonged to the genus Streptomyces. Phylogenetic analysis by 16S rRNA gene sequencing showed a high similarity between MNM-1400 strain and Streptomyces californicus (similarity: 99%). These results suggest that the MNM-1400 strain can be considered as a potential probiotic in aquaculture.
    Description: Issue number on first page of article is incorrect; should be 15(2), not 15(1).
    Keywords: Aquaculture ; Biology ; Fisheries ; Probiotic ; Marine actinobacteria ; Biological control ; Shrimp ; Litopenaeus vannamei ; Biotechnology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 630-644
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  • 5
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    In:  http://aquaticcommons.org/id/eprint/23029 | 18721 | 2018-06-13 23:12:36 | 23029 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-12
    Description: Sponges are the simplest multicellular animals that lack defense mechanisms and rely on chemical defense that have been used by mankind to develop antimicrobial drugs against diseases. The present study was designed to demonstrate the antibacterial and antifungal activities of marine sponge Dysidea pallescens semipolar and nonpolar extracts. In this study, D. pallescens were collected from Hengam Island in the Persian Gulf. The extracts were produced by Bligh and Dyer method. Broth Dilution Methods were used to check the antimicrobial activity of D. pallescens extracts against Escherichia coli (ATCC 15224), Pseudomonas aeruginosa (ATCC 25619), Staphylococcus aureus aureus (ATCC 1764), Bacillus subtilis pizizenii (ATCC 6633), Candida albicans (ATCC 10231) and Aspergillus fumigates (PTCC 5009). The results showed diethyl ether extract has bactericidal activity against S. aureus aureus (MBC=10mg/mL) and B. subtilis spizizenii (MBC=20mg/mL). D. pallescens diethyl ether extract showed a very weak antifungal activity but methanol extract showed fungicidal activity against A. fumigates (MFC=5mg/mL) and C. albicans (MFC=1.5 mg/mL). Therefore nonpolar-semipolar secondary metabolites of D. pallescens solutions in diethyl ether have shown significant antibacterial activity and polar-secondary metabolites solutions in methanol have shown significant antifungal activity.
    Keywords: Biology ; Fisheries ; Health ; Antibacterial ; Antifungal ; Marine sponge ; Persian Gulf ; Biotechnology ; Hengam Island ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 200-209
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  • 6
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    In:  http://aquaticcommons.org/id/eprint/22912 | 18721 | 2018-06-08 19:41:28 | 22912 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-11
    Description: This study was done to evaluate the antimicrobial and antioxidant effects of nisin Z (0.02 %) and sodium benzoate (1.5 and 2.5 %) in vacuum packed Caspian Kutum (Rutilus frisii) fillet stored at 4°C. Microbial changes [aerobic plate counts (APC), psychrotrophic counts (PTC) and lactic acid bacteria] and chemical indices [peroxide value and total volatile basic nitrogen (TVB-N)] were determined in days 0, 4, 8, 12 and 16. Results showed that PV and TVB-N in control samples (the sample dipped in prechilled distilled water) were deteriorated after 12 days compared to preserved samples which were acceptable after 16 days. Microbial tests indicated that control samples contained APC and PTC bacteria in day 16 more than standard limit, whereas treatment samples were in the acceptable range. In case of lactic acid bacteria, after 16 days, all samples were in the acceptable range. Results of chemical and microbial analysis showed that simultaneous use of nisin Z and sodium benzoate could increase the shelf life of vacuum packed R. frisii.
    Keywords: Biology ; Chemistry ; Fisheries ; Rutilus frisii ; Microbial quality ; Lipid oxidation ; Nisin Z ; Sodium benzoate ; Biotechnology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 789-801
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  • 7
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    In:  http://aquaticcommons.org/id/eprint/22837 | 18721 | 2018-05-25 22:34:14 | 22837 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-10
    Description: In this study Escherichia coli DE3 containing expression vector (pET21a) with cloned Persian sturgeon growth hormone (psGH) gene was grown in 10 mL LB broth on a 150 rpm shaker, at the temperature of 37 °C. At the late log phase (determined by OD standard curve) 100 µL isopropyl β-D-1-thiogalactopyranoside (IPTG) was added for induction of GH synthesis. Samples were taken every 2 hours and after bacterial cells lysis crude extracts with recombinant proteins inclusion bodies (IB) were loaded on 15% SDS-PAGE gel. Thenafter staining, comparative concentrations of rpsGH were measured by densitometric scanning of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel it was more than 90 %. The maximum yield of GH was observed after 4 hours of growth. To recover active psGH from inclusion bodies we used imidozole to obtain most of the total recombinant protein in the soluble fraction. Purification of 6xhisN tag recombinant psGH has been performed using affinity chromatography where nickel was bound to an agarose bead by chelation using NTA (nitrilotriacetic acid) beads. The overall yield of the purified monomeric psGH was approximately 50% of the initial IB proteins. The purification manipulations including IB isolation and solubilisation, protein refolding by dialyze and affinity chromatography ensure yields of biologically active psGH up to 30%. This study shows that, the affinity chromatography is a powerful and very specific method for recombinant proteins purification of psGH.
    Keywords: Biology ; Chemistry ; Fisheries ; Persian sturgeon ; Growth hormone ; Purification ; Affinity chromatography ; Biotechnology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 1053-1060
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  • 8
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    In:  http://aquaticcommons.org/id/eprint/23010 | 18721 | 2018-06-13 21:11:25 | 23010 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-12
    Description: The aim of this study was to determine the inhibitory effects of feed ingredients on protease activities of Argyrosomus regius larvae using in vitro techniques. A. regius larvae fed on a commercial feeding procedure were sampled thirteen times, during the sampling period (from 3 to 32 days after hatching (DAH)). The differences observed in protease activities of meagre larvae during the sampling period were statistically significant (p〈0.05). The lowest and highest protease activities of meagre larvae were 5.95±0.6 U/mg protein (15 DAH) and 211.21±12.56 U/mg protein (7 DAH), respectively. The fluctuations observed in protease activities of A. regius larvae were between 10 DAH and 32 DAH. Commercial diets such as Orange Start-S (100-200µ), Orange Start-L (200-300µ), Orange Nurse-XS (300-500µ), Orange Grow-S (300-500µ) and Orange Grow-L (500-800µ) caused the inhibitions on protease activities in meagre larvae to range from 16 to 32 DAH. The results point to the inadequacy of commercial diets such as Orange Grow-S, Orange Grow-L and suitability of Orange Start-S, Orange Start-L, Orange Nurse-XS for feeding meagre larvae during the weaning stage. For the mass production of quality juveniles, future studies should take into account the inhibitory effects of commercial diets and feed ingredients before the manufacturing process. A similar approach may be used to determine the most suitable commercial diets for use during the weaning stages of marine fish larvae to obtain the best growth performance and survival.
    Keywords: Aquaculture ; Biology ; Fisheries ; Meagre ; Argyrosomus regius ; Protease activities ; Protease inhibitions ; Commercial diets ; in vitro ; Biotechnology ; Turkey
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 96-107
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  • 9
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    In:  http://aquaticcommons.org/id/eprint/22808 | 18721 | 2018-05-23 22:09:34 | 22808 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-10
    Description: Alkaline crude enzymes from the viscera of the Tunisian barbel (Barbus callensis) were extracted and characterized. Proteolytic crude extract from barbel viscera was active and stable in alkaline solution. The optimum pH and temperature were 11.0 and 55 °C, respectively, using casein as a substrate. The crude alkaline protease was extremely stable in the pH range of 5.0-12.0. Zymography activity staining using casein as a substrate showed the presence of at least five distinct proteases. The crude alkaline proteases showed stability towards various surfactants, bleach agents and compatibility with some commercial detergents. Alkaline proteases from the viscera of the barbel were tested in chicken feather-degradation and showed important feather degrading activity. Complete solubilisation of whole feathers was observed after 24h of incubation at 50°C. Additionally, crude alkaline protease demonstrated powerful capabilities of hair removal from skin and the collagen, the major leather-forming protein, was not significantly degraded. Considering its promising properties, alkaline crude enzyme from the viscera of the Tunisian barbel may be considered as a potential candidate for future use in several biotechnological processes.
    Keywords: Biology ; Chemistry ; Fisheries ; Digestive protease ; Barbus callensis ; Detergent ; Keratine-degradation ; Dehairing function ; Biotechnology ; Tunisia
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 672-686
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  • 10
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    In:  http://aquaticcommons.org/id/eprint/22801 | 18721 | 2018-05-23 19:30:35 | 22801 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-10
    Description: Some biochemical characteristics of common carp (Cyprinus carpio) and silver carp (Hypophthalmichthys molitrix) surimi prepared by a conventional washing method and protein isolated using alkaline-acid-aided processes were investigated. Solubility of protein in silver carp and common carp were found to be highest by using the conventional washing method. Decreases in myoglobin and lipid contents in both fish were found in the alkaline- or acid-aided process when compared to the conventional process (p〈0.05). The percentage of protein recovery with the alkaline-aided process of silver carp was highest. The whiteness score in the alkaline solubilization process was higher than acid solubilization process and conventional method in silver carp. This result was different than the result obtained in common carp. There was no significant difference between treatments regarding total sulfhydryl (SH) groups (p〉0.05). In protein patterns of Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), the lowest intensity of the myosin heavy chains (MHC) band was found in silver carp by the conventional washing process.
    Keywords: Biology ; Chemistry ; Fisheries ; Common carp ; Silver carp ; Acid-alkaline solubilization ; Recovery of protein ; Biotechnology ; Iran ; biochemical ; Cyprinus carpio ; Hypophthalmichthys molitrix
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 583-597
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