Publication Date:
1999-05-21
Description:
The nucleotide exchange activity of RCC1, the only known nucleotide exchange factor for Ran, a Ras-like small guanosine triphosphatase, was required for microtubule aster formation with or without demembranated sperm in Xenopus egg extracts arrested in meiosis II. Consistently, in the RCC1-depleted egg extracts, Ran guanosine triphosphate (RanGTP), but not Ran guanosine diphosphate (RanGDP), induced self-organization of microtubule asters, and the process required the activity of dynein. Thus, Ran was shown to regulate formation of the microtubule network.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ohba, T -- Nakamura, M -- Nishitani, H -- Nishimoto, T -- New York, N.Y. -- Science. 1999 May 21;284(5418):1356-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Maidashi Higashi-ku, Fukuoka 812-8582, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10334990" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
*Cell Cycle Proteins
;
Cell Extracts
;
Cell Nucleus/metabolism
;
DNA-Binding Proteins/metabolism
;
Dyneins/metabolism
;
Female
;
GTP Phosphohydrolases/*metabolism
;
*Guanine Nucleotide Exchange Factors
;
Guanosine Diphosphate/metabolism
;
Guanosine Triphosphate/*metabolism
;
Male
;
Microtubules/chemistry/*metabolism/ultrastructure
;
Nuclear Proteins/analysis/*metabolism
;
Ovum
;
Recombinant Proteins/metabolism
;
Sperm Head/physiology
;
Spindle Apparatus/chemistry/*metabolism/ultrastructure
;
Xenopus
;
*Xenopus Proteins
;
ran GTP-Binding Protein
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
