Abstract
The nucleotide exchange activity of RCC1, the only known nucleotide exchange factor for Ran, a Ras-like small guanosine triphosphatase, was required for microtubule aster formation with or without demembranated sperm in Xenopus egg extracts arrested in meiosis II. Consistently, in the RCC1-depleted egg extracts, Ran guanosine triphosphate (RanGTP), but not Ran guanosine diphosphate (RanGDP), induced self-organization of microtubule asters, and the process required the activity of dynein. Thus, Ran was shown to regulate formation of the microtubule network.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Cell Cycle Proteins*
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Cell Extracts
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Cell Nucleus / metabolism
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DNA-Binding Proteins / metabolism
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Dyneins / metabolism
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Female
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GTP Phosphohydrolases / metabolism*
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Guanine Nucleotide Exchange Factors*
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Guanosine Diphosphate / metabolism
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Guanosine Triphosphate / metabolism*
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Male
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Microtubules / chemistry
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Microtubules / metabolism*
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Microtubules / ultrastructure
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Nuclear Proteins / analysis
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Nuclear Proteins / metabolism*
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Ovum
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Recombinant Proteins / metabolism
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Sperm Head / physiology
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Spindle Apparatus / chemistry
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Spindle Apparatus / metabolism*
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Spindle Apparatus / ultrastructure
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Xenopus
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Xenopus Proteins*
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ran GTP-Binding Protein
Substances
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Cell Cycle Proteins
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Cell Extracts
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DNA-Binding Proteins
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Guanine Nucleotide Exchange Factors
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NUMA1 protein, Xenopus
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Nuclear Proteins
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RCC1 protein, Xenopus
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Recombinant Proteins
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Xenopus Proteins
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Guanosine Diphosphate
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Guanosine Triphosphate
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GTP Phosphohydrolases
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Dyneins
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ran GTP-Binding Protein