Publication Date:
1999-05-15
Description:
Bone marrow stem cells develop into hematopoietic and mesenchymal lineages but have not been known to participate in production of hepatocytes, biliary cells, or oval cells during liver regeneration. Cross-sex or cross-strain bone marrow and whole liver transplantation were used to trace the origin of the repopulating liver cells. Transplanted rats were treated with 2-acetylaminofluorene, to block hepatocyte proliferation, and then hepatic injury, to induce oval cell proliferation. Markers for Y chromosome, dipeptidyl peptidase IV enzyme, and L21-6 antigen were used to identify liver cells of bone marrow origin. From these cells, a proportion of the regenerated hepatic cells were shown to be donor-derived. Thus, a stem cell associated with the bone marrow has epithelial cell lineage capability.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Petersen, B E -- Bowen, W C -- Patrene, K D -- Mars, W M -- Sullivan, A K -- Murase, N -- Boggs, S S -- Greenberger, J S -- Goff, J P -- New York, N.Y. -- Science. 1999 May 14;284(5417):1168-70.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA. bryon+@pitt.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10325227" target="_blank"〉PubMed〈/a〉
Keywords:
2-Acetylaminofluorene/pharmacology
;
Animals
;
Bone Marrow Cells/*cytology
;
Bone Marrow Transplantation
;
Carbon Tetrachloride/pharmacology
;
Cell Differentiation
;
Cell Division
;
DNA-Binding Proteins/genetics
;
Dipeptidyl Peptidase 4/metabolism
;
Epithelial Cells/cytology
;
Female
;
Hematopoietic Stem Cells/cytology
;
In Situ Hybridization
;
Liver/*cytology/drug effects/physiology
;
*Liver Regeneration
;
Liver Transplantation
;
Male
;
*Nuclear Proteins
;
Polymerase Chain Reaction
;
Rats
;
Rats, Inbred F344
;
Rats, Inbred Lew
;
Sex-Determining Region Y Protein
;
Stem Cells/*cytology
;
*Transcription Factors
;
Y Chromosome
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
