Abstract
Bone marrow stem cells develop into hematopoietic and mesenchymal lineages but have not been known to participate in production of hepatocytes, biliary cells, or oval cells during liver regeneration. Cross-sex or cross-strain bone marrow and whole liver transplantation were used to trace the origin of the repopulating liver cells. Transplanted rats were treated with 2-acetylaminofluorene, to block hepatocyte proliferation, and then hepatic injury, to induce oval cell proliferation. Markers for Y chromosome, dipeptidyl peptidase IV enzyme, and L21-6 antigen were used to identify liver cells of bone marrow origin. From these cells, a proportion of the regenerated hepatic cells were shown to be donor-derived. Thus, a stem cell associated with the bone marrow has epithelial cell lineage capability.
MeSH terms
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2-Acetylaminofluorene / pharmacology
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Animals
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Bone Marrow Cells / cytology*
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Bone Marrow Transplantation
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Carbon Tetrachloride / pharmacology
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Cell Differentiation
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Cell Division
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DNA-Binding Proteins / genetics
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Dipeptidyl Peptidase 4 / metabolism
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Epithelial Cells / cytology
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Female
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Hematopoietic Stem Cells / cytology
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In Situ Hybridization
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Liver / cytology*
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Liver / drug effects
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Liver / physiology
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Liver Regeneration*
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Liver Transplantation
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Male
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Nuclear Proteins*
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Polymerase Chain Reaction
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Rats
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Rats, Inbred F344
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Rats, Inbred Lew
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Sex-Determining Region Y Protein
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Stem Cells / cytology*
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Transcription Factors*
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Y Chromosome
Substances
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DNA-Binding Proteins
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Nuclear Proteins
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Sex-Determining Region Y Protein
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Transcription Factors
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2-Acetylaminofluorene
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Carbon Tetrachloride
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Dipeptidyl Peptidase 4