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Primary culture of ram epididymal epithelial cells (1989)

Hwang, Hank H. ; Healey, Mark C. ; Elsner, Yoko Y. ; [et al.]

Springer

Methods in cell science 12 (1989), S. 77-81

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ISSN: 1573-0603

Keywords: primary culture ; ram lamb epididymitis ; epididymal epithelial cells

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary A procedure for the primary culture of epithelial cells harvested from the epididymides of young virgin ram lambs is described. A confluent monolayer of epitheial cells can be produced from cell aggregates isolated from the caput and cauda segments of ram lamb epididymides after trypsin and collagenase digestions. A culture of enriched epididymal epithelial cells is obtained using a selective attachment procedure. Characteristic epithelial cell morphology can be confirmed by phase contrast and indirect immunofluorescent microscopies. Cultured epithelial cells can be passed 4 times and maintained for 1 mo.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01404648

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