Summary
A procedure for the primary culture of epithelial cells harvested from the epididymides of young virgin ram lambs is described. A confluent monolayer of epitheial cells can be produced from cell aggregates isolated from the caput and cauda segments of ram lamb epididymides after trypsin and collagenase digestions. A culture of enriched epididymal epithelial cells is obtained using a selective attachment procedure. Characteristic epithelial cell morphology can be confirmed by phase contrast and indirect immunofluorescent microscopies. Cultured epithelial cells can be passed 4 times and maintained for 1 mo.
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Hwang, H.H., Healey, M.C., Elsner, Y.Y. et al. Primary culture of ram epididymal epithelial cells. Journal of Tissue Culture Methods 12, 77–81 (1989). https://doi.org/10.1007/BF01404648
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DOI: https://doi.org/10.1007/BF01404648