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Primary culture of ram epididymal epithelial cells

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Journal of tissue culture methods

Summary

A procedure for the primary culture of epithelial cells harvested from the epididymides of young virgin ram lambs is described. A confluent monolayer of epitheial cells can be produced from cell aggregates isolated from the caput and cauda segments of ram lamb epididymides after trypsin and collagenase digestions. A culture of enriched epididymal epithelial cells is obtained using a selective attachment procedure. Characteristic epithelial cell morphology can be confirmed by phase contrast and indirect immunofluorescent microscopies. Cultured epithelial cells can be passed 4 times and maintained for 1 mo.

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Author notes

  1. Hank H. Hwang

    Present address: Immunochemicals Department, Sigma Chemical Company, P.O. Box 14508, 63178, St. Louis, MO

Authors and Affiliations

  1. College of Agriculture, Department of Animal, Dairy and Veterinary Sciences, Utah State University, 84322, Logan, Utah

    Hank H. Hwang, Mark C. Healey, Yoko Y. Elsner & Alice V. Johnston

Authors
  1. Hank H. Hwang
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  2. Mark C. Healey
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  3. Yoko Y. Elsner
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  4. Alice V. Johnston
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Hwang, H.H., Healey, M.C., Elsner, Y.Y. et al. Primary culture of ram epididymal epithelial cells. Journal of Tissue Culture Methods 12, 77–81 (1989). https://doi.org/10.1007/BF01404648

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