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  • Articles  (294)
  • wheat  (294)
  • Springer  (294)
  • 1990-1994  (294)
  • 1
    Electronic Resource
    Electronic Resource
    Effect of experience on in-flight orientation to host-associated cues in the generalist parasitoidLysiphlebus testaceipes (1993)
    Springer
    Entomologia experimentalis et applicata 68 (1993), S. 219-229 
    Details
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Aphidiidae ; Homoptera ; Aphididae ; Schizaphis graminum ; wheat ; tritrophic interactions ; learning ; host-habitat location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of experience on the responsiveness of the aphidiid parasitoidLysiphlebus testaceipes (Cresson) (Hymenoptera: Aphidiidae) to host-associated cues was investigated using a wind-tunnel bioassay. Naive females were able to discriminate between uninfested wheat (Triticum aestivum L.) and wheat infested withSchizaphis gramimum (Rondani) (Homoptera: Aphididae), but oviposition experience significantly increased the parasitoid's propensity to respond to aphid-infested plants with upwind, targeted flight. The behavioural change associated with such experience was acquired rapidly (within five minutes) and persisted for at least 24 h. The parasitoid could be successfully conditioned to associate a novel odour with the presence of hosts, suggesting that the increase in response to aphid-infested plants which occurs as a result of experience is probably due to associative learning of olfactory cues from the plant-aphid complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02380774
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  • 2
    Electronic Resource
    Electronic Resource
    DNA in wheat seeds from European archaeological sites (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 571-575 
    Details
    ISSN: 1420-9071
    Keywords: Ancient DNA ; archaeobotany ; carbonized grain ; DNA sequences ; glutenin alleles ; seed proteins ; Triticum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have used hybridization analysis to detect ancient DNA in wheat seeds collected from three archaeological sites in Europe and the Middle East. One of these samples, carbonizedT. spelta dated to the first millennium BC, has yielded PCR products after amplification with primers directed at the leader regions of the HMW (high molecular weight) glutenin alleles. Sequences obtained from these products suggest that the DNA present in the Danebury seeds is chemically damaged, as expected for ancient DNA, and also indicate that it should be possible to study the genetic variability of archaeological wheat by ancient DNA analysis. Finally, we describe a PCR-based system that enables tetraploid and hexaploid wheats to be distinguished.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01921727
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  • 3
    Electronic Resource
    Electronic Resource
    Mycotoxin formation in HY-320 wheat during granary storage at 15 and 19% moisture content (1990)
    Springer
    Mycopathologia 111 (1990), S. 181-189 
    Details
    ISSN: 1573-0832
    Keywords: mycotoxin ; ochratoxin ; Penicillium ; storage ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Eleven-kilogram parcels of HY-320 wheat, a cultivar of the new Canada Prairie Spring class, were kept at 15 and 19% initial moisture contents (IMC) in simulated storage in a Manitoba farm granary for 60 weeks to determine biotic and abiotic changes and mycotoxin production. Ochratoxin A reached a maximum of 0.24 ppm by week 20 in the 19% IMC wheat, but was absent in the 15% IMC wheat; no other mycotoxins were detected. Temperature, moisture content, O2 and CO2 levels, fat acidity values, seed germination, microfloral incidence and abundance, and the presence of other mycotoxins were monitored. Principal component analysis of all variables showed that the first principal components accounted for 32–41% of the system variability, and contained the ochratoxin A variable. Ochratoxin A was produced in moist grain that had decreased seed germination andAltermaria activity, and high fungal activity byPenicillium andAspergillus versicolor. Compared to other stored cereals previously studied, HY-320 wheat would be ranked in a low-risk category for mycotoxin formation, based on the ochratoxin A levels observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02282802
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of the incubation conditions on the production of patulin by Penicillium griseofulvum isolated from wheat (1991)
    Springer
    Mycopathologia 115 (1991), S. 163-168 
    Details
    ISSN: 1573-0832
    Keywords: Penicillium griseofulvum ; patulin ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Sixty-four wheat samples from Spanish flour factories were screened for patulin and patulin-producing moulds. None of them was found to contain any patulin, whereas samples experimentally contaminated with this toxin proved it to be highly unstable. On the other hand, Penicillium griseofulvum was the only in vitro patulin-producing species found (19 samples). Mould growth in the samples was investigated by using yeast-sucrose medium (YES) and high-performance liquid chromatography (HPLC) to measure the amounts of toxin produced during 40 day's incubation at 20 and 28°C. The highest yield rate of patulin was obtained between the 20th and 30th day of incubation; such a rate, however, was very low throughout the vigorous growth phase, during the first 20 days of incubation. The more appropriate temperature for incubation and patulin production was 28 °C. We also investigated the influence of other incubation conditions in the yield and found stationary dark cultures to be more efficient that shaken or fermentation cultures in YES medium. The best patulin yield achieved was 11.9 mg in the culture broth and 6.3 mg in the mycelium from 100 ml of medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00462220
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  • 5
    Electronic Resource
    Electronic Resource
    Assessing risk associated with N fertilizer recommendations in the absence of soil tests (1994)
    Springer
    Nutrient cycling in agroecosystems 40 (1994), S. 175-183 
    Details
    ISSN: 1573-0867
    Keywords: Nitrogen response ; on-farm research ; risk ; probability ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In many developing countries, fertilizer recommendations must be made in the absence of plant and soil analyses. When the region is variable in terms of soils, weather, and magnitude of response to fertilizer, a recommendation is likely to involve a high degree of risk for the farmer. Quantification of such risk is key to developing appropriate recommendations for the farmer. However, most methodologies generally used in analyzing fertilizer trials do not allow adequate quantification, especially as a continuous function, of the risk associated with a given recommendation. Three years of on-farm trials conducted in the High Valley of Mexico were used to evaluate different methodologies for generating N fertilization recommendations and their associated risk for wheat (Triticum aestivum) production in the absence of soil tests. When the traditional approach, using average yield responses or separate trial results, was used, an economic optimum was identified, but it was not possible to quantify the associated risk. In contrast, however, by using a combination of response surface methodology and simple probability analysis, the risk associated with any given recommendation was developed, even under the highly variable conditions of the study zone. The approach uses a treatment difference matrix (developed using average yield differences between a treatment and the zero N (0N) check) and its associated standard deviation over locations. From the matrix, an equation (being a function of N rate and relative grain:N price ratios) was developed that shows the probability of outperforming the 0N check for the economic optimum rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00750463
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  • 6
    Electronic Resource
    Electronic Resource
    The effect of level of application on the residual value of superphosphate on a sandy soil in south-western Australia (1991)
    Springer
    Nutrient cycling in agroecosystems 29 (1991), S. 163-172 
    Details
    ISSN: 1573-0867
    Keywords: Superphosphate ; residual value ; sandy soil ; leaching of phosphorus ; lupins ; barley ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a field experiment on deep, yellow, sandy soil near Badgingarra, Western Australia, the residual value of superphosphate applied one and two years previously was measured relative to freshly-applied superphosphate using yields of narrow-leafed lupin (Lupinus angustifolius), barley and wheat. In addition, soil samples were collected for measurement of bicarbonate-extractable soil P. This was also used to estimate the residual value of the superphosphate. For lupins and wheat, and for bicarbonate-extractable soil P, the residual value decreased with increasing level of application. For barley grain, the residual value was not significantly affected by the level of application. The decrease in residual value of superphosphate with increasing level of application is attributed to increased leaching of applied phosphorus (P) down the profile of the sandy soils as the level of application increases. This may reduce subsequent plant yields due to the delay in seedling roots reaching the P in the soil during the crucial early stages of plant growth. For lupins, the relationship between yield and the level of superphosphate applied was markedly sigmoidal. The relationship for wheat and barley was exponential. Consequently, at suboptimal levels of P application, lupins required about two to three times more P than wheat or barley to produce the same yield. However, lupins required less P to achieve near-maximum yield.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01048956
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  • 7
    Electronic Resource
    Electronic Resource
    The current and residual value of superphosphate for lupins grown in rotation with oats and wheat on a deep sandy soil (1992)
    Springer
    Nutrient cycling in agroecosystems 31 (1992), S. 319-329 
    Details
    ISSN: 1573-0867
    Keywords: Superphosphate ; fertilizer effectiveness ; residual value ; lupins ; oats ; wheat ; grain production ; bicarbonate-extractable soil phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a field experiment on a deep pale-yellow sand in a 600 mm per annum rainfall Mediterranean environment of south-western Australia, six levels of phosphorus (P) as superphosphate (O up to 546 kg P ha−1) were applied once only, to the soil surface, before sowing lupins (Lupinus angustifolius). The lupins were grown in a continuous arable cropping rotation with, in successive years, oats (Avena sativa), wheat (Triticum aestivum), lupins. Five such rotations were started in the experiment from 1985 to 1989. The experiment continued until the end of 1990. The relationship between lupin seed (grain) yields and the level of P applied was measured in the year of P application for five successive years (1985 to 1989). The relationship had the same general form but it varied between years, largely due to different maximum yields (yield plateaux) in each year. The residual value of superphosphate applied three years previously was measured for lupins on two occasions (1988 and 1989) relative to superphosphate applied in the current year. The residual values was different in the two years. The superphosphate applied three years previously was about 30% as effective as freshly applied superphosphate in 1988, and 12% as effective in 1989. At each harvest, the relationship between grain yield and the P concentration in the grain differed for different species. However, for each species at each harvest, the relationship was similar regardless of when the P was applied in the previous years. Thus each species had the same internal efficiency of P use curve, and yields varied only with P concentration in tissue. Bicarbonate-extractable soil P was determined on soil samples taken in mid-July of 1989 and 1990. These soil test values were related to grain yields at harvest. The relationship between yield and soil test values had the same general form but varied for different species within years and for each species between years. It also varied for each species within years depending on the year the P was applied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01051283
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  • 8
    Electronic Resource
    Electronic Resource
    Residual value of superphosphate for wheat and lupin grain production on a uniform yellow sandplain soil (1992)
    Springer
    Nutrient cycling in agroecosystems 31 (1992), S. 331-340 
    Details
    ISSN: 1573-0867
    Keywords: Superphosphate ; residual value ; wheat ; lupins ; soil test for phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a field experiment on a sandplain soil in a low rainfall (326 mm per annum) Mediterranean environment of south-western Australia, the effectiveness of superphosphate applied in 1986 was measured in three subsequent years relative to freshly-applied superphosphate each year, using grain (seed) yields of wheat (Triticum aestivum) and lupins (Lupinus angustifolius). The wheat and lupins were grown in rotation and both crops were grown each year starting in 1986. Bicarbonate-soluble phosphorus was determined on soil samples taken in mid June from where the P treatment was applied in 1986 only. These soil test values were related to the grain yields produced that year. For each level of superphosphate applied in 1986, soil test values decreased with increasing time from application. The relationship between grain yield and soil test values had the same general form within each year for both plant species, but varied between years. For both species, the effectiveness of superphosphate decreased by about 70–80% between the year of application and the first and second years after application, and by a further approximate 10% in the third year. The relationship between grain yield and the level of superphosphate applied became sigmoidal by 1989.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01051284
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  • 9
    Electronic Resource
    Electronic Resource
    Relationship between electroultrafiltration (EUF) extractable nitrogen, grain yield, and optimum nitrogen fertilizer rates for winter wheat (1992)
    Springer
    Nutrient cycling in agroecosystems 32 (1992), S. 37-43 
    Details
    ISSN: 1573-0867
    Keywords: nitrogen fertilizer rate ; electroultrafiltration ; wheat ; soil test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The objective of the investigation was to examine whether there exist relationships between the optimum nitrogen fertilizer rate for winter wheat and soil nitrogen fractions extracted by electroultrafiltration (EUF) from autumn samples of the upper soil layer (0–30 cm). Optimum nitrogen fertilizer rates were derived from grain yield curves of field trials carried out with increasing nitrogen fertilizer rates on 19 different sites in 1985/86 and 1986/87. Most soils were luvisols derived from loess, two soils were brown earths and one a pararendzina. Total Nitrogen fertilizer rates were 0, 40, 80, and 120 kg N/ha applied twice before ear emergence. The final nitrogen rate at ear emergence was the same for all treatments, namely 60 kg N/ha. Optimum nitrogen fertilizer rates were derived from the grain yield curve fitted to a modified Mitscherlich equation. The optimum nitrogen fertilizer rates were correlated with the nitrogen fractions extracted by EUF. The regression equation thus obtained showed that NO 3 - , the organic N fraction (EUF Norg), and the EUF Norg-quotient each had a highly significant impact on the optimum nitrogen fertilizer rate. The higher the amounts of EUF-N extracted the lower the optimum nitrogen rate. Substituting the EUF Norg-fraction for total nitrogen concentration in the upper soil layer gave a poorer relationship between the optimum nitrogen fertilizer rate and the soil data. In absolute terms the EUF Norg-fraction had by far the greatest impact on calculating the optimum nitrogen fertilizer rate. The investigation shows that the EUF method is a suitable technique for the determination of available soil nitrogen from which optimum nitrogen fertilizer rates can be derived for winter wheat cultivated under soil and climatic conditions typical for cereal growing areas in central Europe.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01054392
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  • 10
    Electronic Resource
    Electronic Resource
    Effect of soil pH on the requirement for water-soluble phosphorus in triple superphosphate fertilizers (1994)
    Springer
    Nutrient cycling in agroecosystems 40 (1994), S. 207-214 
    Details
    ISSN: 1573-0867
    Keywords: available P ; citrate insoluble P ; phosphorus sources ; triple superphosphate ; Triticum aestivum ; water soluble P ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A greenhouse study was conducted to determine if soil pH affects the requirement for water-soluble P and the tolerance of water-insoluble impurities in TSP fertilizers. Two commercial TSP fertilizers were selected to represent a range in phosphate rock sources and impurities. Phosphate fertilizer impurities were isolated as the water-washed fraction by washing whole fertilizers with deionized water. TSP fertilizers with various quantities of water-soluble P (1.2 to 99% water-soluble P) were simulated by mixing the water-washed fertilizer fractions or dicalcium phosphate (DCP) with reagent-grade monocalcium phosphate (MCP). The fertilizers were applied to supply 40 mg AOAC available P kg−1 to a Mountview silt loam (fine-silty, siliceous, thermic Typic Paleudults). Wheat (Triticum aestivum (L.)) was harvested at 49 and 84 days after planting. Soil pH values at the final forage harvest were 5.4±0.16 and 6.4±0.15. At a soil pH of 5.4, the TSP fertilizers required only 37% water-soluble P to reach maximum yields while at pH 6.4 the fertilizers required 63% water-soluble P. Results of this study show that higher levels of water -insoluble P can be tolerated in TSP fertilizers when applied to acid soils. Phosphorus uptake was not affected by soil pH, but for the mixtures containing the fertilizer residues the source having the lowest level of Fe and Al had a higher relative agronomic effectiveness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00750467
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  • 11
    Electronic Resource
    Electronic Resource
    Critical level of DTPA extractable Zn for wheat in alkaline soils of semiarid region of Punjab, India (1990)
    Springer
    Nutrient cycling in agroecosystems 21 (1990), S. 163-166 
    Details
    ISSN: 1573-0867
    Keywords: Critical level of Zn ; alkaline soils ; Zn-deficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field experiments were conducted at 32 locations, chosen for their wide range in DTPA extractable Zn, to determine the critical deficiency level of Zn for predicting response of wheat to Zn application. Soil application of 5.6 kg Zn ha−1 significantly increased the grain yield in deficient soils. Soil extractable Zn was significantly related with per cent grain response and absolute grain yield. Both the graphical and statistical methods of Cate and Nelson indicated the critical level to be 0.75 mg kg−1 soil of DTPA extractable Zn. This level gave a predictability value of 82 per cent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01087426
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  • 12
    Electronic Resource
    Electronic Resource
    Studies on the effectiveness of various sulfur fertilizers under controlled conditions (1990)
    Springer
    Nutrient cycling in agroecosystems 22 (1990), S. 147-159 
    Details
    ISSN: 1573-0867
    Keywords: Sulfate ; elemental sulfur ; leaching ; ryegrass ; wheat ; greenhouse experiments ; split application
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Three factorial experiments with four replications were conducted in a greenhouse to examine the effectiveness of gypsum, elemental sulfur (ES powder) and three S containing N fertilizers, viz., ammonium sulfate (AS), urea + ES, and Ureas (20% AS and 80% urea). All experiments were conducted twice in different years. In the first experiment with uncropped soil, the effects of soil type, leaching rate (2.3 and 6.9 mm water per day) and urea addition on sulfate leaching losses were studied. Leaching losses decreased in the order Ureas 〉 ammonium sulfate (AS) 〉 gypsum ≫ urea + ES. Increasing the leaching rate greatly increased sulfate losses from both soils. Losses were greater in the sandy Typic Hapludoll than in the clayey Oxic Paleustalf. Sulfate adsorption was found to decrease strongly with rising the pH in both soils. Hydrolysis of urea temporarily raised the pH of the soil, thereby increasing the sulfate leaching losses. In the second experiment the effects of S rate (0–65 mg per kg soil), split application and leaching rate (0 and 2.3 mm per day) on sulfate leaching losses and ‘apparent S recovery’ (ASR) by three successive cuts of ryegrass (Lolium perenne L.) were studied. Herbage yield more than doubled when S was applied. The effectiveness of the sulfate fertilizers was greater when S was split-applied than given all at once. With split applications the ASR decreased in the order: Ureas 〉 AS 〉 gypsum 〉 urea + ES 〉 ES powder. ES fertilizers were least effective, because the oxidation rate of ES to sulfate was clearly too slow. In the third experiment the effects of S rate (0–40 mg per kg soil) and split application on sulfate leaching losses and ASR in the grain of wheat (Triticum aestivum L.) were studied under leaching conditions (2.3 mm per day). Grain yield increased strongly due to S application. Split application greatly increased the effectiveness of the sulfate fertilizers and appeared to be an effective tool in satisfying the S need of the crop under leaching conditions. Again, ES fertilizers were least effective, because the oxidation rate of ES was too slow to meet the S demand of the crop. In all experiments leaching losses of sulfate from the ES fertilizers were smaller than from the sulfate fertilizers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01120390
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  • 13
    Electronic Resource
    Electronic Resource
    Placing superphosphate at different depths in the soil changes its effectiveness for wheat and lupin production (1990)
    Springer
    Nutrient cycling in agroecosystems 22 (1990), S. 97-107 
    Details
    ISSN: 1573-0867
    Keywords: Superphosphate ; placement depth ; banding ; relative effectivenesss ; lupins ; wheat ; field experiment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a field experiment on a sandplain soil in a low rainfall (326mm per annum) Mediterranean environment of south-western Australia, seven levels of single superphosphate, 0, 7.5, 10, 14, 19.5, 30 and 39 kg P ha−1, were placed at either 3, 5, 7, 9, 11 or 13 cm depth before sowing wheat (Triticum aestivum) at 3 cm. In a separate treatment, superphosphate was drilled with the seed (the normal practice). In the second year, the plots were sown with lupins (Lupinus angustifolius) at 3 cm depth with no additional superphosphate. In three separate treatments, superphosphate at 0, 14 and 39 kg P ha−1, was drilled with the lupin seed (the normal practice) on plots that had received no superphosphate in the first year. Yields of wheat and lupins were used as a measure of the effectiveness of the superphosphate placement treatments relative to the effectiveness of superphosphate drilled with seed of wheat (year 1) or lupins (year 2), to give relative effectiveness (RE) values in each of the two years. In the first year the RE of superphosphate was increased by about 20% when the fertilizer was placed 5 to 9 cm deep in the soil. In the second year, the RE of superphosphate for producing lupin grain was increased by about 30–60% where the fertilizer had been placed 5–13 cm deep in the previous year compared with freshly drilled 3 cm deep. The yield of wheat or lupins was closely related to the P content of plant tissue; each relationship was independent of the depth or year of superphosphate application.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01116183
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  • 14
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    The effect of level of nitrogen nutrition upon mineral content and removal in grasses and wheat (1990)
    Springer
    Nutrient cycling in agroecosystems 26 (1990), S. 229-235 
    Details
    ISSN: 1573-0867
    Keywords: Grass ; wheat ; nitrogen nutrition ; dilution curve ; mineral content ; mineral removal ; phosphorus ; potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The important effect of nitrogen in changing the patterns of mineral content and mineral removal is analysed for grass swards and wheat. Different models are proposed; accumulated dry matter developed throughout a growing period is shown to be an excellent reference for assessing the evolution of the plant mineral content and the mineral removal the growing crop. Applications in diagnosing mineral nutrition status and optimising fertilizer use are proposed and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01048760
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  • 15
    Electronic Resource
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    Wheat growth from phosphorus fertilizers as affected by time and method of application in soil with an acidic subsurface layer (1994)
    Springer
    Nutrient cycling in agroecosystems 39 (1994), S. 77-82 
    Details
    ISSN: 1573-0867
    Keywords: Acidic subsurface layer ; application rate ; North Carolina phosphate rock ; placement method ; time of application ; triple superphosphate ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using soils with an acidic subsurface layer, three glasshouse experiments were carried out to evaluate the effect of placement method and application rate of triple superphosphate (TSP) and North Carolina phosphate rock (NCPR) on dry matter (DM) yields. Time of application of NCPR on DM yield response of wheat was also studied. For Experiment 1, soil was collected in depth intervals of 0–2; 4–6; 6–8; and 8–10 cm from a red earth (chromic luvisol). The treatments included two P sources (TSP and NCPR), three placement methods (broadcast, banded or mixed into the subsurface layer, 6–8 cm), and six application rates. In this P deficient soil with an acidic subsurface layer, there was relatively little effect of application method of TSP on wheat yield responses. The maximum dry matter yield responses for broadcast, band and mix application methods was 30, 42 and 50 %, respectively. Responses to NCPR broadcast, band and mix methods were 20, 9 and 44 %, respectively. Mixing NCPR into to acidic subsurface layer produced yields similar to those from TSP although a higher application rate of P as NCPR was needed to achieve this outcome. Treatments for Experiments 2 and 3 were time of application of NCPR (0 and 30 days before sowing) and rate of application of NCPR (0 and 40 mg P/pot). In Experiment 2 (same soil as Experiment 1) application of NCPR prior to sowing, resulted in higher Colwell P concentration than when applied at sowing, but time of application had no effect on final DM yields. Experiment 3 used a red podzolic (chromic luvisol) soil which had a lower P-status, was more acid and had a lower exchangeable Ca2+ concentration than the red earth. Application of NCPR prior to sowing resulted in lower DM yield than when it was applied prior to sowing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00750159
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  • 16
    Electronic Resource
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    Effect of superphosphate and nitrogen on yield and take-all of wheat (1992)
    Springer
    Nutrient cycling in agroecosystems 31 (1992), S. 43-49 
    Details
    ISSN: 1573-0867
    Keywords: Superphosphate ; nitrogen fertilizer ; take-all ; (Gaeumannomyces graminis) ; wheat ; Triticum aestivum L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat was grown continuously in soil amended with 5 levels of superphosphate and with 4 levels of urea at 3 sites. The incidence and severity of take-all, caused byGaeumannomyces graminis var.tritici, declined with increasing rates of application of both superphosphate and urea. In both years, the severity of take-all on plants receiving neither superphosphate nor urea was about 40% while at the highest level of superphosphate and urea supply the take-all severity was approximately halved at 22%. There was an increase in grain yield in response to applied superphosphate and urea to the highest level of each nutrient. There was also an increase in the 1,000-kernal weights with superphosphate and urea fertilizer application.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01064226
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  • 17
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    The phosphorus requirement of different crop species compared with wheat on lateritic soils (1992)
    Springer
    Nutrient cycling in agroecosystems 32 (1992), S. 27-36 
    Details
    ISSN: 1573-0867
    Keywords: Phosphorus requirement ; wheat ; oats ; barley ; lupins ; triticale ; superphosphate ; grain yield ; phosphorus in grain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The phosphorus (P) requirement for grain production of different crop species (oats (Avena sativa), barley (Hordeum vulgare), triticale (xTriticosecale), narrow-leafed lupins (Lupinus angustifolius), and sandplain lupins (L. cosentinii) was compared with wheat (Triticum aestivum) in five field experiments on different lateritic soils in south-western Australia. Seven or eight levels of superphosphate were applied at the start of each experiment. The amount of P required to produce 70% (four experiments) or 90% (one experiment) of the maximum yield was used to compare P requirements. Large differences in the P requirements of the species were obtained. On P deficient soil in 3 experiments, oats required from 50 to 70% less P than wheat, but required 40% more P on a soil with a long history of superphosphate applications. Compared with wheat, in the year of P application, barley required 50% less P in one experiment, had similar P requirements in two experiments, and required 80% more P in another experiment. In the years after P application, barley required 20% less P in two experiments. On an acidic soil triticale required from 50% to 70% less P than wheat, but on less acidic soil it required 100% more P. In the year of P application, narrow-leafed lupins required 800% more P than wheat in one experiment, and 30% more P in the other experiment. In the year of P application, sandplain lupins required 70% less P than wheat in one experiment.
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    URL: http://dx.doi.org/10.1007/BF01054391
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    Phosphorus effectiveness in fertilized soils evaluated by chemical solutions and residual value for wheat growth (1992)
    Springer
    Nutrient cycling in agroecosystems 32 (1992), S. 185-194 
    Details
    ISSN: 1573-0867
    Keywords: Residual P ; P fixation ; Bray P ; Olsen P ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nineteen soils from the south east of the Province of Buenos Aires (Argentina) that had been fertilized with moderate amounts of P (10–40 kgP/ha) during the last 10 years were used to investigate the effect of time on the decline of P availability as measured by three soil tests (Bray 1, Bray 2, Olsen) and the null-point method. Differences in rates of P decline among soils and chemical methods were characterized by an exponential coefficient for time (b 2) in equations which describe the changes of the added P retained by the soil (Pr =ac b1 t b2). The rate of decline of P for the nineteen soils calculated for the soil test methods was ordered decreasingly as: null-point 〉 Olsen 〉 Bray 1 〉 Bray 2. The ability of the chemical methods for assessing the residual value of P for wheat growth (RV) was tested in a pot experiment on seven of the soils that differed in their individual rates of reaction with P. Differences between soils in the rate of reaction with P as measured in the laboratory by the null-point method and by the Olsen test were reflected in different residual values for P fertilizer for wheat plants. Thus the value ofb 2 for these methods was well correlated with the observed residual values. The soil properties commonly associated with the retention of P were not related to the value ofb 2 suggesting that more than one soil property may be involved in the measure ofb 2. The exponent for timeb 2 may be used as an index of the ability of the soil test to reflect the decline of P availability with time.
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    URL: http://dx.doi.org/10.1007/BF01048781
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    The residual effectiveness of previously applied copper fertiliser for grain yield of wheat grown on soils of south-west Australia (1994)
    Springer
    Nutrient cycling in agroecosystems 39 (1994), S. 11-18 
    Details
    ISSN: 1573-0867
    Keywords: Copper fertiliser ; nitrogen fertiliser ; residual effectiveness ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The residual effectiveness of copper (Cu) applied 18 to 21 years previously was estimated for grain yield of wheat. In one field experiment, current levels of Cu fertiliser were applied and its effectiveness was compared to that of the same level of Cu applied previously. The effects of nitrogen (N) fertiliser on the Cu concentration in the youngest emerged blade and in the grain, as well as the effects of N levels on the grain yield of wheat, were also studied. Where the recommended level of Cu fertiliser had been applied previously, its residual effectiveness depended on the soil type. On the grey sands over clay and gravelly sands over clay, the residual Cu would last approximately 20 years where wheat is grown in rotation with a legume crop (Lupinus augustifolius L.) and where N fertiliser is applied at high levels (92 kg N ha−1). On the yellow brown sandy earths of the Newdegate district, the residual value was in excess of 30 years. When Cu levels in the soil are marginal, high levels of N applied to wheat crops grown on stubbles of legume crops (high soil N) could suffer from induce Cu deficiency which could reduce grain production. Critical concentrations of Cu in the youngest emerged blade of less than 1.2 mg Cu kg−1 at Gs50–59 would indicate Cu deficiency. Cu concentrations of less than 1.1–1.2 mg Cu kg−1 in the grain suggest that the wheat crop is marginally supplied with Cu. In both situations, Cu fertiliser needs to be applied before the next crop.
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    URL: http://dx.doi.org/10.1007/BF00750152
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    Penicillium bilaji and phosphorus fertilization effects on the growth, development, yield and common root rot severity of spring wheat (1994)
    Springer
    Nutrient cycling in agroecosystems 39 (1994), S. 97-103 
    Details
    ISSN: 1573-0867
    Keywords: Cochliobolus sativus ; common root rot ; Penicillium bilaji ; phosphorus fertilization ; tillering ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A strain ofPenicillium bilaji Chalabuda (PB) has recently been commercially released as a seed inoculant to increase phosphorus (P) uptake by wheat (Triticum aestivum L.). The purpose of this study was to compare the effects of drill applied P (15 kg P ha−1) with PB seed inoculation on early growth, development, P uptake, and grain yield of ‘Stoa’ spring wheat at four sites in North Dakota. Fertilization with P consistently enhanced early season growth, main stem development, tillering and P uptake. Seed inoculation with PB had little or no effect on these traits. Phosphorus fertilization tended to increase common root rot severity (CRR, incited byCochliobolus sativus (Ito & Kurib) Drechs.), while PB inoculation had no effect. Grain yields were significantly increased by P fertilization at one location. Inoculation with PB also increased grain yield at this location. The reason why PB inoculation increased yield at this location is not evident, as plant growth and P uptake were not enhanced earlier in the season. Averaged across all four sites, PB inoculation increased wheat yields 66 kg ha−1, which is similar to averaged yield responses reported from the Prairie Provinces of Canada.
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    URL: http://dx.doi.org/10.1007/BF00750908
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    Effect of feeding cereal-legume diets to rats on in vitro absorption of45Ca (1990)
    Springer
    Cellular and molecular life sciences 46 (1990), S. 1016-1017 
    Details
    ISSN: 1420-9071
    Keywords: In vitro absorption ; calcium ; wheat ; Bengal gram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The in vitro absorption of calcium from the duodenum was significantly less in a group of rats fed on a wheat diet than in a group fed a wheat and Bengal gram (70∶30) diet.
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    URL: http://dx.doi.org/10.1007/BF01940659
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    A segment of rye chromosome 1 enhances growth and embryogenesis of calli derived from immature embryos of wheat (1991)
    Springer
    Plant cell reports 10 (1991), S. 148-151 
    Details
    ISSN: 1432-203X
    Keywords: wheat ; rye ; embryogenesis ; growth ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The influence of the short arm of rye chromosome 1 (1RS) from Secale cereale var. Imperial on the growth and differentiation of callus cultures from wheat Triticum aestivum var. Chinese Spring immature embryos was analysed. This chromosome arm was found to stimulate both embryogenesis and the rate of growth of calli. Recombinant lines carrying segments of 1RS were used to delineate the regions of 1RS responsible for the tissue culture effects. The enhancement of embryogenesis and the stimulation of growth were shown to be associated with two distinct genetic regions of the chromosome arm; the former is located between the centromere and the Sec 1 locus, while the latter is situated in the immediate vicinity of the Sec 1 locus.
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    URL: http://dx.doi.org/10.1007/BF00232047
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    Allelochemicals in soil from no-tillage versus conventional-tillage wheat (Triticum aestivum) fields (1990)
    Springer
    Journal of chemical ecology 16 (1990), S. 2277-2289 
    Details
    ISSN: 1573-1561
    Keywords: Allelochemicals ; no-tillage ; conventional-tillage ; soils ; wheat ; Triticum aestivum ; mass spectrometry ; Petri-dish bioassay ; fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Putative allelochemicals found in the soil of no-tillage and conventional-tillage wheat plots near Stillwater, Oklahoma, were obtained by a mild alkaline aqueous extraction procedure, bioassayed to determine their biological activity, purified, and analyzed with a capillary gas chromatography-mass spectrometry-data analysis system. The most significant inhibition was found in bioassays of extracts from soil collected immediately after harvest in June, July, and August. No-tillage soils produced significant inhibition during the rest of the year also. Mass spectrometry showed fatty acids as the most abundant compounds. However, when bioassayed authentic samples of the five free fatty acids showed no significant biological activity toward wheat.
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    URL: http://dx.doi.org/10.1007/BF01026937
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  • 24
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    Allelopathic activity in wheat-conventional and wheat-no-till soils: Development of soil extract bioassays (1992)
    Springer
    Journal of chemical ecology 18 (1992), S. 2191-2221 
    Details
    ISSN: 1573-1561
    Keywords: Cover crops ; wheat ; Triticum aestivum ; soybean ; Glycine max ; soil extracts ; germination bioassays ; phenolic acids ; hydroxamic acids ; allelopathy ; slope analysis ; ivy-leaved morning glory ; Ipomoea hederacea ; crimson clover ; Trifolium incarnalum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The primary objective of this research was to determine if soil extracts could be used directly in bioassays for the detection of allelopathic activity. Here we describe: (1) a way to estimate levels of allelopathic compounds in soil; (2) how pH, solute potential, and/or ion content of extracts may modify the action of allelopathic compounds on germination and radicle and hypocotyl length of crimson clover (Trifolium incarnatum L.) and ivyleaved morning glory (Ipomoea hederacea L. Jacquin.); and (3) how biological activity of soil extracts may be determined. A water-autoclave extraction procedure was chosen over the immediate-water and 5-hr EDTA extraction procedures, because the autoclave procedure was effective in extracting solution and reversibly bound ferulic acid as well as phenolic acids from wheat debris. The resulting soil extracts were used directly in germination bioassays. A mixture of phenolic acids similar to that obtained from wheat-no-till soils did not affect germination of clover or morning glory and radicle and hypocotyl length of morning glory. The mixture did, however, reduce radicle and hypocotyl length of clover. Individual phenolic acids also did not inhibit germination, but did reduce radicle and hypocotyl length of both species. 6-MBOA (6-methoxy-2,3-benzoxazolinone), a conversion product of 2-o-glucosyl-7-methoxy-1,4-benzoxazin-3-one, a hydroxamic acid in living wheat plants, inhibited germination and radicle and hypocotyl length of clover and morning glory. 6-MBOA, however, was not detected in wheat debris, stubble, or soil extracts. Total phenolic acids (FC) in extracts were determined with Folin and Ciocalteu's phenol reagent. Levels of FC in wheat-conventionaltill soil extracts were not related to germination or radicle and hypocotyl length of either species. Levels of FC in wheat-no-till soil extracts were also not related to germination of clover or morning glory, but were inversely related to radicle and hypocotyl length of clover and morning glory. FC values, solute potential, and acidity of wheat-no-till soil extracts appeared to be independent (additive) in action on clover radicle and hypocotyl length. Radicle and hypocotyl length of clover was inversely related to increasing FC and solute potential and directly related to decreasing acidity. Biological activity of extracts was determined best from slopes of radicle and hypocotyl length obtained from bioassays of extract dilutions. Thus, data derived from the water-autoclave extraction procedure, FC analysis, and slope analysis for extract activity in conjunction with data on extract pH and solute potential can be used to estimate allelopathic activity of wheat-no-till soils
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    URL: http://dx.doi.org/10.1007/BF00984946
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    Nucleotide sequence of a cDNA coding for the barley seed protein CMa: an inhibitor of insect α-amylase (1992)
    Springer
    Plant molecular biology 18 (1992), S. 423-427 
    Details
    ISSN: 1573-5028
    Keywords: Hordeum vulgare L. ; CM protein ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The primary structure of the insect α-amylase inhibitor CMa of barley seeds was deduced from a full-length cDNA clone pc43F6. Analysis of RNA from barley endosperm shows high levels 15 and 20 days after flowering. The cDNA predicts an amino acid sequence of 119 residues preceded by a signal peptide of 25 amino acids. Ala and Leu account for 55% of the signal peptide. CMa is 60–85% identical with α-amylase inhibitors of wheat, but shows less than 50% identity to trypsin inhibitors of barley and wheat. The 10 Cys residues are located in identical positions compared to the cereal inhibitor family with a Pro-X-Cys motif present in all.
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    URL: http://dx.doi.org/10.1007/BF00034972
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    Homologues of wheat ubiquitin-conjugating enzymes - TaUBC1 and TaUBC4 are encoded by small multigene families in Arabidopsis thaliana (1994)
    Springer
    Plant molecular biology 24 (1994), S. 651-661 
    Details
    ISSN: 1573-5028
    Keywords: protein degradation ; ubiquitin conjugating enzymes ; DNA repair ; N-end recognition ; wheat ; Arabidopsis thaliana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Covalent attachment of ubiquitin to other cellular proteins has been implicated in a multitude of diverse physiological processes in eukaryotes including selective protein degradation. This attachment is carried out by a multi-enzyme pathway consisting of three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin-protein ligases (E3s). E2s accept activated ubiquitin from E1 and conjugate it to target proteins with or without the participation of specific E3s. Previously, we have isolated wheat cDNAs encoding 16 and 23 kDa E2s, TaUBC1 and TaUBC4, respectively. TaUBC1 shows structural homology to the yeast RAD6 E2 that is essential for DNA repair whereas TaUBC4 is related to the yeast ScUBC8 E2, both of which effectively conjugate ubiquitin to histones in vitro but as yet are without a known in vivo function. Here, we report the isolation of genomic and cDNA homologues of these genes from Arabidopsis thaliana. In Arabidopsis, both of these E2s are encoded by three member gene families. Members of the AtUBC1 gene family, comprising AtUBC1, 2 and 3, encode 150–152 amino acid proteins that are 83–99% identical to each other and TaUBC1 and contain four introns that are conserved with respect to position. Members of the AtUBC4 gene family, comprising AtUBC4, 5 and 6, encode 187–191 amino acid proteins that are 73–88% identical to each other and TaUBC4 and contain five introns that are conserved with respect to position. In contrast, AtUBC1-3 gene products are only 31–36% identical to those derived from AtUBC4-6. mRNA for each family was detected in Arabidopsis roots, leaves, stems, and flowers indicating that members of each family are expressed in most if not all tissues.
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    URL: http://dx.doi.org/10.1007/BF00023561
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    Analysis of the gibberellin-responsive promoter of a cathepsin B-like gene from wheat (1992)
    Springer
    Plant molecular biology 20 (1992), S. 849-856 
    Details
    ISSN: 1573-5028
    Keywords: GA regulation ; thiol-protease promoter ; wheat ; aleurone ; particle gun
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A wheat gene (A121) encoding a protein with sequence similarity to mammalian cathepsin B is regulated by gibberellic acid (GA) in aleurone layers of germinating grains. To analyse the mechanism of A121 regulation, its promoter was fused to the β-glucuronidase reporter gene (GUS) and introduced by micro-projectile bombardment into aleurone layers of oat. With 2.3 kb of promoter sequence, the GUS expression was enhanced by GA treatment. This effect was reversed by abscisic acid (ABA). This result showed for A121, like the α-amylase genes, that the regulation by GA and ABA was at the level of transcription. The GA responsiveness of the promoter was retained with as little as 276 bp of promoter sequence. Sequence comparison with a GA responsive promoter of an α-amylase gene identified the conserved element GCAACGGCAACGATGG which is required intact for full expression of both promoters. However, there was no identifiable similarity in the cathepsin-like promoter with the GA-responsive element of α-amylase promoters with the consensus sequence TAACAAA, suggesting that GA affects more than one mechanism of transcriptional control.
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    URL: http://dx.doi.org/10.1007/BF00027156
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    Sequence analysis of WIS-2-1A, a retrotransposon-like element from wheat (1992)
    Springer
    Plant molecular biology 20 (1992), S. 991-995 
    Details
    ISSN: 1573-5028
    Keywords: retrotransposon-like element ; sequence analysis ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract WIS-2-1A, a 8624 bp insertion in the Glu-1A-2 locus of chromosome 1A of wheat, consists of two 1755 bp long terminal repeats enclosing a 5114 bp internal region. No long open reading frames could be found, but inspection of the predicted amino acid sequence showed regions with homology to retrotransposon structures, including a methionine tRNA initiator binding site, a nucleotide binding domain, a protease, an integrase and a polymerase. DNA replication errors have resulted in frame-shifts in the protein coding region, suggesting that retrotransposition of WIS-2-1A, if it occurs, must be mediated by trans-acting factors.
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    URL: http://dx.doi.org/10.1007/BF00027169
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    Single-seed PCR of LMW glutenin genes to distinguish between durum wheat cultivars with good and poor technological properties (1993)
    Springer
    Plant molecular biology 22 (1993), S. 1173-1176 
    Details
    ISSN: 1573-5028
    Keywords: polymerase chain reaction ; LMW glutenin genes ; wheat ; quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polymerase chain reaction (PCR) was used to amplify low-molecular-weight (LMW) glutenin sequences from genomic DNA extracted from a single germinating seed of several durum wheat genotypes. Electrophoretic analysis of PCR reactions showed the presence of amplified products characteristic of durum wheat cultivars with good and poor technological properties. This PCR-based approach is proposed as a very efficient and safe alternative to standard procedures for selecting durum wheat genotypes with good qualitative characteristics.
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    URL: http://dx.doi.org/10.1007/BF00028988
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    The amino acid sequence of a protein from wheat kernel closely related to proteins involved in the mechanisms of plant defence (1993)
    Springer
    The protein journal 12 (1993), S. 379-386 
    Details
    ISSN: 1573-4943
    Keywords: Amino acid sequence ; wheat ; wounding ; plant defence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The amino acid sequence of wheatwin1, a monomeric protein of 125 residues isolated from wheat kernel (variety S. Pastore), is reported. Wheatwin1 is highly homologous (95%) to barwin, a protein from barley seed, which was shown to be related to the C-terminal domain of two proteins encoded by the wound-induced geneswin1 andwin2 in potato and to a protein encoded by the same domain of the hevein gene (hev1) in rubber tree. Similarly to barwin, wheatwin1 contains six cysteine residues all linked in disulfide bridges and the N-terminal residue is pyroglutamate. Moreover, structural studies performed on wheatwin1 andwin1 protein by predictive methods demonstrated that these proteins and barwin are closely related in the secondary structure also. The high level of homology found with the product ofwin1,win2, andhev1 genes strongly suggests that barwin and wheatwin1 play a common role in the mechanism of plant defence.
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    In vitro processing of transcripts containing novel tRNA-like sequences (‘t-elements’) encoded by wheat mitochondrial DNA (1990)
    Springer
    Plant molecular biology 15 (1990), S. 551-559 
    Details
    ISSN: 1573-5028
    Keywords: tRNA-like sequences ; t-elements ; RNA processing ; mitochondria ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have recently described the properties of a wheat mitochondrial extract that is able to process, accurately and efficiently, artificial transcripts containing wheat mitochondrial tRNA sequences, with the production of mature tRNAs (P.J. Hanic-Joyce and M.W. Gray, J. Biol. Chem., in press). Such processing involves 5′-endonucleolytic, 3′-endonucleolytic, and TRNA nucleotidyltransferase activities. Here we show that this system also acts on transcripts containing sequences corresponding to an unusual class of short repeats (‘t-elements’) in wheat mtDNA. These repeats are theoretically capable of assuming a tRNA-like secondary structure, although stable transcripts corresponding to them are not detectable in vivo. We find that t-element sequences are processed with the same specificity and with comparable efficiency as are authentic tRNA sequences. Because known t-elements are located close to and in the same transcriptional orientation as active genes (18S-5S, 26S, tRNAPro) in wheat mtDNA, our results raise the question of whether t-elements play a role in gene expression in wheat mitochondria.
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    Characterization of members of a pseudogene subfamily of the wheat α-gliadin storage protein genes (1991)
    Springer
    Plant molecular biology 16 (1991), S. 335-337 
    Details
    ISSN: 1573-5028
    Keywords: Triticum ; wheat ; endosperm ; gliadin ; pseudogene ; duplication ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00020565
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    Wheat ubiquitin gene exhibits a conserved protein coding region and a diverged 3′ non-coding region (1991)
    Springer
    Plant molecular biology 16 (1991), S. 907-908 
    Details
    ISSN: 1573-5028
    Keywords: ubiquitin ; wheat ; heat shock protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00015082
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    Site-directed mutagenesis and expression inEscherichia coli of WMAI-1, a wheat monomeric inhibitor of insect α-amylase (1991)
    Springer
    Plant molecular biology 17 (1991), S. 1005-1011 
    Details
    ISSN: 1573-5028
    Keywords: α-amylase inhibitor ; expression inE. coli ; glycosylation versus activity ; insect α-amylase ; mutagenesis ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The wheat monomeric inhibitor WMAI-1 (syn. 0.28) produced inEscherichia coli using the pT7-7 expression ventor has the correct N-terminal sequence and the same electrophoretic mobility and specific activity towards the α-amylase from the insectTenebrio molitor as the native WMAI-1 isolated from wheat. This confirms that the native inhibitor is not glycosylated and contradicts claims that a putative glycosyl moiety was essential for inhibition. Thirteen mutants have been obtained at six different sites. Substitution of the highly conserved N-terminal S by the sequence ARIRAR increased the pre-incubation time required for maximum activity. A similar result was obtained by insertion of GPRLPW after position 4, while insertion of EPRAPW at the same position rendered the inhibitor inactive. The substitution D/EGPRL and insertions DGP or D, at position 58, produced complete inactivation. All other mutations had only minor effects on activity.
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    A Triticum aestivum cDNA clone encoding a low-molecular-weight heat shock protein (1991)
    Springer
    Plant molecular biology 17 (1991), S. 273-275 
    Details
    ISSN: 1573-5028
    Keywords: Tahsp17.3 ; low-molecular-weight HSP ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00039504
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    Unusual sequence of group 3 LEA (II) mRNA inducible by dehydration stress in wheat (1993)
    Springer
    Plant molecular biology 21 (1993), S. 907-912 
    Details
    ISSN: 1573-5028
    Keywords: abscisic acid ; dehydration ; LEA ; water stress ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone, pMA1949, detects two mRNA species in wheat seedling tissue that are late embryogenesis-abundant (LEA) and dehydration stress-inducible. Sequence analysis of the pMA1949 clone shows it to be a 991 bp partial cDNA encoding a polypeptide of 317 amino acids with homology to two group 3 LEA proteins, carrot (DC8) and a soybean protein encoded by pGmPM2 cDNA. Molecular analysis of the deduced protein reveals a 33 kDa acidic and extremely hydrophilic protein with potential amphiphilic α-helical regions. In addition, the protein contains eleven similar, contiguous repeats of 11 amino acids, which are separated by 118 amino acids from two additional and unique repeats of 36 residues each at the carboxyl end of the protein. Comparisons of sequences of reported group 3 LEA proteins revealed that there are two types, separable by sequence similarity of the 11 amino acid repeating motifs and by the presence or absence of a certain amino acid stretch at the carboxyl terminus. Based on resuls from these comparisons, we propose a second type of group 3 LEA proteins, called group 3 LEA (II).
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    Wheat acetyl-CoA carboxylase (1993)
    Springer
    Plant molecular biology 22 (1993), S. 547-552 
    Details
    ISSN: 1573-5028
    Keywords: acetyl-CoA carboxylase ; chloroplast ; haloxyfop ; herbicide ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The acetyl-CoA carboxylase present in both wheat germ and total wheat leaf protein contains ca. 220 kDa subunits. It is the major biotin-dependent carboxylase present in wheat chloroplasts. Active acetyl-CoA carboxylase purified from wheat germ is a homodimer with an apparent molecular mass of ca. 500 kDa. The enzyme from wheat germ or from wheat chloroplasts is sensitive to the herbicide haloxyfop at micromolar levels. The incorporation of 14C-acetate into fatty acids in freshly cut wheat seedling leaves provides a convenient in vivo assay for both acetyl-CoA carboxylase and haloxyfop.
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    Purification and characterization of (1→3, 1→4)-β-glucan endohydrolases from germinated wheat (Triticum aestivum) (1993)
    Springer
    Plant molecular biology 22 (1993), S. 847-859 
    Details
    ISSN: 1573-5028
    Keywords: amino acid sequence ; cDNA ; germination ; (1→3, 1→4)-β-glucanases ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A (1→3, 1→4)-β-glucan 4-glucanohydrolase [(1→3, 1→4)-β-glucanase, EC 3.2.1.73] was purified to homogeneity from extracts of germinated wheat grain. The enzyme, which was identified as an endohydrolase on the basis of oligosaccharide products released from a (1→3, 1→4)-β-glucan substrate, has an apparent pI of 8.2 and an apparent molecular mass of 30 kDa. Western blot analyses with specific monoclonal antibodies indicated that the enzyme is related to (1→3, 1→4)-β-glucanase isoenzyme EI from barley. The complete primary structure of the wheat (1→3, 1→4)-β-glucanase has been deduced from nucleotide sequence analysis of cDNAs isolated from a library prepared using poly(A)+ RNA from gibberellic acid-treated wheat aleurone layers. One cDNA, designated λLW2, is 1426 nucleotide pairs in length and encodes a 306 amino acid enzyme, together with a NH2-terminal signal peptide of 28 amino acid residues. The mature polypeptide encoded by this cDNA has a molecular mass of 32085 and a predicted pI of 8.1. The other cDNA, designated λLW1, carries a 109 nucleotide pair sequence at its 5′ end that is characteristic of plant introns and therefore appears to have been synthesized from an incompletely processed mRNA. Comparison of the coding and 3′-untranslated regions of the two cDNAs reveals 31 nucleotide substitutions, but none of these result in amino acid substitutions. Thus, the cDNAs encode enzymes with identical primary structures, but their corresponding mRNAs may have originated from homeologous chromosomes in the hexaploid wheat genome.
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    Isolation and analysis of a cDNA clone encoding the small subunit of ADP-glucose pyrophosphorylase from wheat (1993)
    Springer
    Plant molecular biology 23 (1993), S. 23-33 
    Details
    ISSN: 1573-5028
    Keywords: wheat ; Triticum aestivum ; cDNA clone ; ADP-glucose pyrophosphorylase ; small-subunit ; starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A full-length cDNA clone from hexaploid bread wheat, encoding the small subunit of ADP-glucose pyrophosphorylase, has been isolated from an endosperm cDNA library. The cDNA insert has an open reading frame which encodes a protein of 473 amino acids (52.1 kDa). The presence of a chloroplast/amyloplast transit peptide of 22 amino acids is proposed. The deduced amino acid sequence exhibits a high degree of homology with the small subunit ADP-glucose pyrophosphorylase proteins from rice (with 90% of identical amino acids) and potato (with 86% of identical amino acids) and contains conserved sequence elements which are thought to represent the substrate binding and allosteric activator sites. The genes are organised as single-copy loci on chromosomes 7A, 7B and 7D in the wheat genome and are highly expressed during grain development. Homologous transcripts are expressed in leaves and roots.
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    The amino acid sequence and reactive site of a single-headed trypsin inhibitor from wheat endosperm (1994)
    Springer
    The protein journal 13 (1994), S. 187-194 
    Details
    ISSN: 1573-4943
    Keywords: Trypsin inhibitor ; wheat ; primary structure ; reactive site ; Bowman-Birk
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The sequence of a trypsin inhibitor, isolated from wheat endosperm, is reported. The primary structure was obtained by automatic sequence analysis of the S-alkylated protein and of purified peptides derived from chemical cleavage by cyanogen bromide and digestion withStaphylococcus aureus V8 protease. This protein, named wheat trypsin inhibitor (WTI), which is comprised of a total of 71 amino acid residues, has 12 cysteines, all involved in disulfide bridges. The primary site of interaction (reactive site) with bovine trypsin has been identified as the dipeptide arginyl-methionyl at positions 19 and 20. WTI has a high degree of sequence identity with a number of serine proteinase inhibitors isolated from both cereal and leguminous plants. On the basis of the findings presented, this protein has been classified as a single-headed trypsin inhibitor of Bowman-Birk type.
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    Cloning of cDNA and chromosomal location of genes encoding the three types of subunits of the wheat tetrameric inhibitor of insect α-amylase (1990)
    Springer
    Plant molecular biology 14 (1990), S. 845-853 
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    ISSN: 1573-5028
    Keywords: α-amylase tetrameric inhibitor ; cDNA cloning ; genetic mapping ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have characterized three cDNA clones corresponding to proteins CM1, CM3 and CM16, which represent the three types of subunits of the wheat tetrameric inhibitor of insect α-amylases. The deduced amino acid sequences of the mature polypeptides are homologous to those of the dimeric and monomeric α-amylase inhibitors and of the trypsin inhibitors. The mature polypeptides are preceded by typical signal peptides. Southern blot analysis of appropriate aneuploids, using the cloned cDNAs as probes, has revealed the location of genes for subunits of the CM3 and of the CM16 type within a few kb of each other in chromosomes 4A, 4B and 4D, and those for the CM1 type of subunit in chromosomes 7A, 7B and 7D. Known subunits of the tetrameric inhibitor corresponding to genes from the B and D genomes have been previously characterized. No proteins of this class have been found to be encoded by the A genome in hexaploid wheat (genomes AA, BB, DD) or in diploid wheats (AA) and no anti α-amylase activity has been detected in the latter, so that the A-genome genes must be either silent (pseudogenes) or expressed at a much lower level.
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    Cloning and sequencing of cDNAs encoding a pathogen-induced putative peroxidase of wheat (Triticum aestivum L.) (1991)
    Springer
    Plant molecular biology 16 (1991), S. 329-331 
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    ISSN: 1573-5028
    Keywords: pathogen-induced ; peroxidase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report here the complete amino acid sequence of a pathogen-induced putative peroxidase from wheat (Triticum aestivum L.) as deduced from cDNA clones representing mRNA from leaves infected with the powdery mildew fungus Erysiphe graminis. The protein consists of 312 amino acids, of which the first 22 form a putative signal sequence, and has a calculated pI of 5.7. Sequence comparison revealed that the putative wheat peroxidase is most similar to the turnip (Brassica rapa) peroxidase, with which it shares 57% identical and 13% conserved amino acids.
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    Sequence and tissue-specific expression of a putative peroxidase gene from wheat (Triticum aestivum L.) (1991)
    Springer
    Plant molecular biology 16 (1991), S. 171-174 
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    ISSN: 1573-5028
    Keywords: peroxidase gene ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used a cDNA clone encoding a pathogen-induced putative wheat peroxidase to screen a genomic libary of wheat (Triticum aestivum L. cv. Cheyenne) and isolated one positive clone, lambda POX1. Sequence analysis revealed that this clone contains a gene encoding a putative peroxidase with a calculated pI of 8.1 which exhibits 58% and 83% sequence identity to the amino acid sequence of the turnip (Brassica rapa) peroxidase and a pathogen-induced putative wheat peroxidase, respectively. The two introns in the wheat gene are at the same positions as introns in the peroxidase genes of tomato and horseradish. Results of S1-mapping experiments suggest that this gene is neither pathogen-nor wound-induced in leaves but is constitutively expressed in roots.
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    Sequence analysis of a cDNA encoding a Group 3 LEA mRNA inducible by ABA or dehydration stress in wheat (1991)
    Springer
    Plant molecular biology 16 (1991), S. 1073-1076 
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    ISSN: 1573-5028
    Keywords: abscisic acid ; dehydration ; LEA ; water stress ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone (pMA2005) of a Group 3 LEA (late embryogenesis abundant) protein has been sequenced from wheat. The wheat cDNA clone codes for a protein with ten tandem repeats of an 11 amino acid sequence and has homology to other Group 3 LEAs reported in barley, carrot, cotton and rape (L. Dure et al., Plant Mol Biol 12: 475–486, 1989). The deduced amino acid sequence indicates that the wheat protein has a molecular weight of 23 000 and is a basic, hydrophilic protein. Northern analysis with the cDNA clone shows that dehydration of wheat shoot tissue results in increased transcript levels that correlate with increases in endogenous ABA.
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    A chimeric gene (orf256) is expressed as protein only in cytoplasmic male-sterile lines of wheat (1994)
    Springer
    Plant molecular biology 26 (1994), S. 535-539 
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    ISSN: 1573-5028
    Keywords: cytoplasmic male sterility ; coxI ; mitochondria ; membrane protein ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondria derived from Triticum timopheevi have a chimeric gene, orf256, immediately upstream from coxI. Antibodies to a peptide corresponding to a part of the encoded amino acid sequence of orf256 detect a 7 kDa protein on western blots of mitochondrial proteins from cytoplasmic male-sterile (cms) wheat (T. aestivum nucleus, T. timopheevi mitochondria) but not in mitochondrial proteins from T. aestivum, T. timopheevi, or cms plants restored to fertility by introduction of nuclear genes for fertility restoration. The 7 kDa protein appears to serve as a marker for cms wheat. Its occurrence as an integral protein of the inner membrane may indicate a cms effect through an influence on mitochondrial membrane function.
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    Sharp divergence between wheat and barley at loci encoding novel members of the trypsin/α-amylase inhibitors family (1994)
    Springer
    Plant molecular biology 26 (1994), S. 1231-1236 
    Details
    ISSN: 1573-5028
    Keywords: chromosome mapping ; inhibitors of trypsin/α-amylases ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Amino acid sequences for three members (CMx1, CMx2, and CMx3) of a new subfamily of trypsin/α-amylase inhibitors in wheat have been deduced from the nucleotide sequences of the corresponding cDNAs. A cDNA clone encoding CMx1 was selected from a wheat developing endosperm library using a probe that encoded barley trypsin inhibitor BTI-CMe at low stringency. Sequences corresponding to CMx2 and CMx3 were obtained from cDNA amplified by the polymerase chain reaction. The three CMx sequences contain a premature stop codon after 363 nt, as well as a second stop codon at the same position as in BTI-CMe (nt 439–441). Southern analysis of DNAs from diploid, tetraploid, and hexaploid wheats, as well as from aneuploid lines, indicate that there is a single CMx locus in each of the three genomes of hexaploid wheat, respectively associated with chromosomal arms 4AS, 4BS, and 4DL. These genes are expressed early during endosperm development and not expressed at detectable levels in other tissues. Evolutionary implications are discussed.
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    Characterization of the wheat mitochondrial orf25 gene (1990)
    Springer
    Plant molecular biology 15 (1990), S. 793-795 
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    ISSN: 1573-5028
    Keywords: mitochondrial gene ; ORF25 ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The wheat mitochondrial orf25 nucleotide sequence of 576 pb has been determined. Its derived protein sequence shares 88% and 75% amino acid identity with those of maize and tobacco mitochondria, respectively. The wheat and tobacco orf25 sequences lack four inserts, of 6 bp to 36 bp, that are present in the maize homologue. The wheat orf25 gene is actively transcribed and is preceded by a regulatory sequence block very similar to those located upstream of the wheat coxII and atp6 genes. Our observations support the view that orf25 sequences encode a functional polypeptide in plant mitochondria.
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    A novel proline-rich protein from wheat (1991)
    Springer
    Plant molecular biology 16 (1991), S. 663-670 
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    ISSN: 1573-5028
    Keywords: gene expression ; proline-rich protein ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich proteins. The WPRP1 cDNA clone was used to determine the copy number and chromosomal location of the WPRP1 gene by restriction fragment length polymorphism analysis of wheat inbred lines. Although WPRP1 is encoded by a single-copy gene it is also a representative of a larger family of related sequences. RNA gel blot analysis showed that expression of WPRP1 is highest in rapidly growing tissue which together with its amino acid composition suggests a structural role for the encoded protein.
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    A wheat glutathione-S-transferase gene with transposon-like sequences in the promoter region (1991)
    Springer
    Plant molecular biology 16 (1991), S. 1089-1091 
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    ISSN: 1573-5028
    Keywords: wheat ; glutathione-S-transferase ; transposon-like sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00016083
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    Nucleotide sequence of a wheat (Triticum aestivum L.) cDNA clone encoding the waxy protein (1991)
    Springer
    Plant molecular biology 16 (1991), S. 1099-1101 
    Details
    ISSN: 1573-5028
    Keywords: wheat ; Triticum aestivum ; cDNA clone ; waxy protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00016086
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    Structure and sequence of a wheat phosphoribulokinase gene (1991)
    Springer
    Plant molecular biology 17 (1991), S. 167-168 
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    ISSN: 1573-5028
    Keywords: chloroplast ; monocot gene ; phosphoribulokinase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00036823
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    Nucleotide sequence of a Triticum aestivum cDNA clone which is homologous to the 26 kDa chloroplast-localized heat shock protein gene of maize (1991)
    Springer
    Plant molecular biology 17 (1991), S. 255-258 
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    ISSN: 1573-5028
    Keywords: Tahsp26.6 ; chloroplast-localized HSP ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00039500
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    Analysis of nuclear proteins interacting with a wheat α/β-gliadin seed storage protein gene (1993)
    Springer
    Plant molecular biology 22 (1993), S. 25-41 
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    ISSN: 1573-5028
    Keywords: gliadin storage proteins ; wheat ; transcriptional regulation ; nuclear proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The promoter region (−524 to −46) of the wheat α/β-gliadin seed storage protein gene was analyzed for interactions with nuclear proteins from developing wheat seeds. Six complexes were detected within the first 165 bp upstream of the transcriptional start site. One of the proteins was a non-sequence specific AT-binding protein. The remaining five proteins bound in a sequence specific manner. One (CABP) mapped to a conserved CA-rich element at −134 to −112 while another (PalBP) mapped to an adjacent, palindromic sequence at −112 to −106. Three proteins (CTBPs 1–3) formed complexes at two, independent homologous sites. The activities of four of the binding proteins, CTBPs 1–3 and CABP, exhibited similar patterns of expression during seed development: they first appeared at early to mid stages, reached a maximum at mid stage and subsequently decreased, paralleling the pattern of gliadin mRNA accumulation. The non-specific AT-binding protein was detected at relatively high levels only at mid development. PalBP activity, on the other hand, first appeared at mid stage and was present at a constant level throughout later stages of development. The results suggest that the binding proteins may regulate gliadin expression in an antagonistic manner.
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    A light- and developmentally-regulated DNA-binding interaction is common to the upstream sequences of the wheat Calvin cycle bisphosphatase genes (1993)
    Springer
    Plant molecular biology 22 (1993), S. 507-516 
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    ISSN: 1573-5028
    Keywords: Calvin cycle genes ; DNA-binding proteins ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have characterised a DNA-binding interaction common to the upstream sequences of the wheat fructose-1,6-bisphosphatase (FBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) genes. The recognition site for this sequence-specific binding activity, designated wheat FBPase factor (WF-1), is located within 125 bp of the transcription start site of each gene. Within these regions there are no sequence motifs similar to those shown to be important for light-regulated expression in other species. The binding activity was not detected in wheat root nuclear extracts, or in pea leaf extracts. There was a higher level of binding activity in light-grown than in dark-grown wheat leaves. The level was also found to decline when light-grown plants were given an extended dark treatment, but could be reinduced by light. Utilising the gradient of developmental maturity which exists within the wheat leaf it was found that WF-1 activity increases during leaf development.
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    Isolation and characterization of a cDNA clone encoding the TATA box-binding protein (TFIID) from wheat (1992)
    Springer
    Plant molecular biology 19 (1992), S. 867-872 
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    ISSN: 1573-5028
    Keywords: TATA box ; TFIID ; transcription factor ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We isolated a complementary DNA (cDNA) encoding the TATA-binding factor ‘TFIID’ from a wheat seedling cDNA library. The wheat TFIID transcript of 1.2 kb poly(A)+ RNA was expressed at a low level early in germination, but gradually increased as the seedlings developed. In vitro binding experiments showed that the bacterially expressed wheat TFIID protein could specifically bind to the TATA boxes of the cauliflower mosaic virus (CaMV) 35S, wheat histone H3 and adenovirus major late genes with different affinity. A comparison with Arabidopsis TFIID showed the presence of a plant-specific region consisting of 13 amino acids at the divergent amino terminus and a conserved region (182 amino acids) at the carboxy terminus longer than that observed in yeasts (180 amino acids) and animals (181 amino acids).
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    Nucleotide sequence of the internal transcribed spacer region of rDNA in diploid wheat,Triticum speltoides L. (Tausch) Gren. ex Richter (Gramineae) (1992)
    Springer
    Plant molecular biology 20 (1992), S. 157-158 
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    ISSN: 1573-5028
    Keywords: rRNA ; PCR ; ITS ; DNA sequence ; nucleotide ; Triticum speltoides ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00029158
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    Nucleotide sequence of the internal transcribed spacer region of rDNA in wheat,Triticum aestivum L. (Gramineae) (1992)
    Springer
    Plant molecular biology 20 (1992), S. 159-160 
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    ISSN: 1573-5028
    Keywords: rRNA ; PCR ; ITS ; DNA sequence ; nucleotide ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00029159
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    Sequence and organization of a 7.2 kb region of wheat mitochondrial DNA containing the large subunit (26S) rRNA gene (1992)
    Springer
    Plant molecular biology 20 (1992), S. 347-352 
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    ISSN: 1573-5028
    Keywords: mitochondrial DNA ; repeated sequences ; ribosomal RNA ; t-elements ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report the sequence of a 7.2 kilobase pair DNA fragment containing a copy of the wheat mitochondrial gene (rrn26) that encodes the mitochondrial large-subunit ribosomal RNA (26S rRNA). The mature 26S rRNA was determined by direct RNA sequencing to be 3467 nucleotides long, and to share a 5′-terminal pentanucleotide (5′-AUCAU), thought to be important in post-transcriptional processing, with the wheat mitochondrial small-subunit (18S) rRNA. Two other prominent features of the sequence were noted. First, upstream of rrn26 are located two tandem copies of a 70 base pair element containing a putative mitochondrial promoter motif (TCGTATAAAAA). Second, downstream of rrn26 is a sequence element that, if transcribed, would produce and RNA with a secondary structure resembling that of tRNAs but differing sufficiently from the latter structure to preclude any transcript from functioning normally in translation. These upstream and downstream sequence elements may play a role in the expression of rrn26 in wheat mitochondria.
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    A ribosomal protein S10 gene is found in the mitochondrial genome in Solanum tuberosum (1994)
    Springer
    Plant molecular biology 25 (1994), S. 743-749 
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    ISSN: 1573-5028
    Keywords: apocytochrome b pseudogene ; pea cox1 ; plant mitochondria ; potato ; S10 ribosomal protein ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The S10 ribosomal protein gene (rps10), which has not been previously reported in any angiosperm mitochondrial genome, was identified by sequence analysis in the potato mitochondrial DNA. This gene is found downstream of a truncated non-functional apocytochrome b (cob) pseudogene, and is expressed as multiple transcripts ranging in size from 0.8 to 5.0 kb. Southern hybridization analysis indicates that rps10-homologous sequences are not present in the wheat mitochondrial genome. Sequence analysis of a single-copy region of the pea mitochondrial genome located upstream of cox1 [11] shows that a non-functional rps10 pseudogene is present in this species. These results suggest that the functional genes coding for wheat and pea mitochondrial RPS10 polypeptides have been translocated to the nucleus.
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    Dynamical behavior of psb gene transcripts in greening wheat seedlings. I. Time course of accumulation of the psbA through psbN gene transcripts during light-induced greening (1992)
    Springer
    Plant molecular biology 20 (1992), S. 695-704 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; gene expression ; photosystem 2 ; transcription ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The time course of the accumulation of the transcripts from 13 psb genes encoding a major part of the proteins composing photosystem II during light-induced greening of dark-grown wheat seedlings was examined focusing on early stages of plastid development (0.5 h through 72 h). The 13 genes can be divided into three groups. (1) The psbA gene is transcribed as a single transcript of 1.3 kb in the dark-grown seedlings, but its level increases 5- to 7-fold in response to light due to selective increase in RNA stability as well as in transcription activity. (2) The psbE-F-L-J operon, psbM and psbN genes are transcribed as a single transcript of 1.1 kb, two transcripts of 0.5 and 0.7 kb and a single transcript of 0.3 kb, respectively, in the dark-grown seedlings. The levels of accumulation of every transcript remain unchanged or rather decrease during plastid development under illumination. (3) The psbK-I-D-C gene cluster and psbB-H operon exhibit fairly complicated northern hybridization patterns during the greening process. When a psbC or psbD gene probe was used for northern hybridization, five transcripts differing in length were detected in the etioplasts from 5-day old dark-grown seedlings. After 2 h illumination, two new transcripts of different length appeared. Light induction of new transcripts was also observed in the psbB-H operon.
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    Triticum aestivum puroindolines, two basic cystine-rich seed proteins: cDNA sequence analysis and developmental gene expression (1994)
    Springer
    Plant molecular biology 25 (1994), S. 43-57 
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    ISSN: 1573-5028
    Keywords: cDNA sequence ; cystine-rich proteins ; gene expression ; puroindolines ; tryptophan-rich domain ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract From a mid-maturation seed cDNA library we have isolated cDNA clones encoding two Triticum aestivum puroindolines. Puroindoline-a and puroindoline-b, which are 55% similar, are basic, cystine-rich and tryptophan-rich proteins. Puroindolines are synthezised as preproproteins which include N- and C-terminal propeptides which could be involved in their vacuolar localization. The mature proteins have a molecular mass of 13 kDa and a calculated isoelectric point greater than 10. A notable feature of the primary structure of puroindolines is the presence of a tryptophan-rich domain which also contains basic residues. A similar tryptophan-rich domain was found within an oat seed protein and a mammalian antimicrobial peptide. The ten cysteine residues of puroindolines are organized in a cysteine skeleton which shows similarity to the cysteine skeleton of other wheat seed cystine-rich proteins. Northern blot analysis showed that puroindoline genes are specifically expressed in T. aestivum developing seeds. No puroindoline transcripts as well as no related genes were detected in Triticum durum. The identity of puroindolines to wheat starch-granule associated proteins is discussed as well as the potential role of puroindolines in the plant defence mechanism.
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    URL: http://dx.doi.org/10.1007/BF00024197
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    The genetical control of tissue-specific peroxidases,Per-1,Per-2,Per-3,Per-4, andPer-5 in wheat (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 305-313 
    Details
    ISSN: 1432-2242
    Keywords: Peroxidase ; Isoelectric focusing ; Hexaploid ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Isoelectric focusing (IEF) of extracts from different tissues of hexaploid wheat cv “Chinese Spring” provided a method of distinguishing and identifying the four known, and one newly discovered, sets of genes encoding peroxidase isozyme production.Per-1, carried on the short arms of homoeologous group 1 chromosomes, shows a high degree of conservation and is active in coleoptile tissue.Per-2, carried on the short arms of group 2 chromosomes, shows some polymorphism and is most active in root tissue.Per-3, on the long arms of group 3 chromosomes, is highly variable and most active in embryo tissue.Per-4, carried on chromosome arms7AS,4AL, and7DS, is quite variable and most active in endosperm tissue. (The chromosome nomenclature used in this paper is that agreed to by the 7th International Wheat Genetics Symposium, where the previous designations of4A and4B were reversed.) Restriction fragment length polymorphism (RFLP)-based maps of the group 7 chromosomes were used to locatePer-A4 to a distal region of7AS. In addition, a further set of genes was identified as being active in root tissue. In wheat a single locus,Per-D5, was found on chromosome arm2DS.
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    The significance of the magnesium to manganese ratio in plant tissues for growth and alleviation of manganese toxicity in tomato (Lycopersicon esculentum) and wheat (Triticum aestivum) plants (1990)
    Springer
    Plant and soil 124 (1990), S. 205-210 
    Details
    ISSN: 1573-5036
    Keywords: Lycopersicon esculentum L. ; magnesium-manganese interaction ; magnesium: manganese ratio ; manganese toxicity ; tomato ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Results are reported for tomato (Lycopersicon esculentum L. var. Ailsa craig) and wheat (Triticum aestivum L. cv. Mara) which demonstrate that increasing concentrations of Mg in the plant raises plant tolerance to Mn toxicity. Water culture experiments with tomato show that under conditions of high Mn supply (200 µM, Mn), not only does increasing Mg application (0.75 mM to 15 mM) depress Mn uptake, but the higher Mg concentrations in the shoot counteract the onset of Mn toxicity when the concentrations of Mn in the shoot are also high. The ratio of Mg: Mn in the tissues is a better indicator of the appearance of toxicity symptoms than Mn concentration alone. Toxicity symptoms were observed when the Mg:Mn ratio in the shoot tissue was from 1.13 to a value between 3.53 and 6.54. The corresponding Mg: Mn ratio in the older leaves was from 0.82 to between 2.27 and 3.51. For wheat grown in soil, analyses of leaves revealed that growth could be expressed by the following relationship: Y=A+B exp(-kX), where Y=growth, X=Mg:Mn ratio, A, B and k=constants. Growth was significantly reduced when the Mg:Mn ratio fell below 20:1. From a measurement of this ratio it is therefore possible to predict the appearance of Mn toxicity and its influence on growth.
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    Influence of potassium nutrition on concentrations of water soluble carbohydrates, potassium, calcium, and magnesium and the osmotic potential in sap extracted from wheat (Triticum aestivum) ears during preanthesis development (1990)
    Springer
    Plant and soil 124 (1990), S. 281-285 
    Details
    ISSN: 1573-5036
    Keywords: calcium ; ear development ; magnesium ; potassium ; tissue press sap ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract For floret development and final grain number ear elongation in wheat (10–17d before anthesis) is the most sensitive period to adverse growth conditions. Sugars are supposedly the main factor determining floret numbers and grain set, and play next to potassium a significant role in establishing turgor pressure in young tissues. In view of this osmotic function, the influence of K on the concentration of water soluble carbohydrates (WSC, total of sucrose and reducing sugars), Ca and Mg was investigated in pot experiments. Further, the osmotic potential of sap extracted from young ears was determined and compared to sap extracted from the leaf blade. Plants supplied with low amounts of K (moderate K-deficiency) had a considerably lower K concentration in the press sap of the flag leaf and the ear than plants well supplied with K. Concentrations of WSC, Ca and Mg were higher in press sap of the flag leaf in K deficient plants than in plants adequately supplied with K. This indicates a substitution of K in its osmotic role. In press sap from ears, however, WSC, Ca and Mg were not influenced by the K application. Therefore, substances other than those measured must have been responsible for the osmoregulation in the young ear. WSC and the osmotic potential increased (more negative) independently on K supply during ear elongation, while K, Ca and Mg concentrations decreased. Whereas grain number was not influenced by the treatment, single grain weight at maturity was reduced by low K availability in the soil.
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    Modelling potassium uptake by wheat (Triticum aestivum) crops (1990)
    Springer
    Plant and soil 124 (1990), S. 303-307 
    Details
    ISSN: 1573-5036
    Keywords: models ; potassium uptake ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spring wheat was grown in the field under deficient and sufficient levels of soil K and with high and low supplies of fertiliser nitrogen. Measurements were made of K uptake, soil nutrient supply parameters, root growth and, in solution culture, root influx parameters. Mechanistic models predicted uptake reasonably well under K-deficient conditions, but over-predicted uptake, by as much as 4 times, under K-sufficient conditions. The over-prediction was apparently due to poor characterisation of plant demand.
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    URL: http://dx.doi.org/10.1007/BF00009277
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    Effects of nitrogen sources and copper levels on yield, nitrogen and copper contents of wheat (Triticum aestivum L.) (1990)
    Springer
    Plant and soil 126 (1990), S. 79-83 
    Details
    ISSN: 1573-5036
    Keywords: Cu levels ; N sources ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A pot experiment was conducted, in a greenhouse, at Hisar, India, using a sandy soil deficient in nitrogen and copper, to study the effects of various levels of N and Cu on the dry matter yield and the N and Cu contents of wheat (Triticum aestivum L.). The sources of nitrogen used were Ca(NO3)2, NH4Cl and NH4NO3 applied in amounts necessary to establish 120 ppm of soil nitrogen and using a control (0 ppm N). Copper was applied, as copper chloride, to give soil Cu levels of 0, 5, 10 and 20 ppm. In general, dry matter yields, N and Cu concentrations in shoots and roots and available soil-N after harvest of the plants, followed the order Ca(NO3)〉NH4NO3〉NH4Cl. Up to a level of 5 ppm Cu, the dry matter yields of shoots and roots increased, but decreased at higher levels of Cu. Increasing Cu levels significantly decreased the available soil-nitrogen after harvest and also the concentration of N in the plants. At the same time the concentration of Cu in shoots and roots and available Cu in the soil was increased. Nitrogen and copper were found to have a mutually antagonistic effect on each other's concentration in the plants. The antagonism was greater with NH4 + sources than with NO3 − compounds.
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    Evaluation of various methods for the detection of barley yellow dwarf virus by the tissue-blot immunoassay and its use for virus detection in cereals inoculated at different growth stages (1994)
    Springer
    European journal of plant pathology 100 (1994), S. 71-80 
    Details
    ISSN: 1573-8469
    Keywords: wheat ; Triticum aestivum ; oat ; Avena sativa ; barley ; Hordeum vulgare ; serology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Various modifications of the tissue-blot immunoassay (TBIA) for the detection of barley yellow dwarf virus (BYDV, luteovirus) were compared. Similar results were obtained by using three different labelled molecules; goat anti-rabbit antibodies conjugated to alkaline phosphatase, protein A conjugated with alkaline phosphatase and goat anti-rabbit antibodies conjugated with colloidal gold. Blocking the nitrocellulose membrane with polyvinyl alcohol for 1 min was effective and allowed the procedure to be shortened by one hour. TBIA was sensitive enough to detect BYDV in old dry tissue wich had been soaked in water for 1 h. BYDV was monitored by TBIA in wheat, oat and barley after inoculation at heading, flowering and grain filling growth stages. The later the inoculation date, the greater the chance of detecting the virus in stem bases rather than in the upper part of the stem. The later the inoculation the less virus moved, from the inoculated tiller to other tillers of the same plant.
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    URL: http://dx.doi.org/10.1007/BF01871967
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    Fusarium head blight and mycotoxin contamination of wheat, a review (1990)
    Springer
    European journal of plant pathology 96 (1990), S. 187-198 
    Details
    ISSN: 1573-8469
    Keywords: fusarium head blight ; Fusarium culmorum ; F. graminearum ; wheat ; mycotoxin ; deoxynivalenol ; nivalenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Aaraantasting van tarwe doorFusarium culmorum enFusarium graminearum leidt tot vorming van mycotoxinen in het graan, waarvan deoxynivalenol (DON) en nivalenol (NIV) de belangrijkste toxinen zijn. In dit artikel wordt een overzicht gegeven van de toxicologische aspecten, en het voorkomen van deze toxinen in tarwe. Informatie over DON en NIV in tarwe in West-Europa is schaars. Gebaseerd op gegevens vanFusarium epidemieën in de jaren 1979–1986 wordt een schatting gegeven van de concentratie DON in Nederlandse tarwe. Rekening houdend met de herkomst en verwerking van tarwe, blijken zowel in dierlijk als menselijk voedsel lage concentraties DON chronisch voor te komen. Op basis van een maximaal toelaatbare dagelijkse dosis DON van 3 μg kg−1 lichaamsgewicht is de schatting van de dagelijkse opname van DON in het jaar volgend op de oogst van 1982 net op de grens. Zowel een jaarlijkse inventarisatie vanFusarium aantasting en DON besmetting van het graan, als de ontwikkeling vanFusarium-resistente rassen zijn noodzakelijk.
    Notes: Summary An infection of bread wheat by fusarium head blight contaminates the crop with mycotoxins, particularly deoxynivalenol (DON) and nivalenol (NIV). The toxicity and natural occurrence of these mycotoxins in wheat are reviewed. Based on 8 years data of fusarium head blight epidemics of wheat in the Netherlands, DON contamination of the grain was estimated. Fusarium head blight ratings averaged an infection of 1.7% of all spikelets; estimates for DON contamination averaged 0.9 mg kg−1. Taking a guideline level for DON in uncleaned bread wheat of 2 mg kg−1, in 1979 and 1982 a wheat crop was produced with estimated DON concentrations above the limit of tolerance. Human and animal exposure to mycotoxins in the Netherlands appears to be small but chronic. The information presented in this paper illustrates the need for an annual evaluation of the crop for fusarium head blight incidence and mycotoxin content, and the necessity of fusarium head blight resistant wheat cultivars.
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    URL: http://dx.doi.org/10.1007/BF01974256
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    Effect of cereal-legumechapati diets on absorption and retention of calcium (1992)
    Springer
    Plant foods for human nutrition 42 (1992), S. 165-173 
    Details
    ISSN: 1573-9104
    Keywords: Calcium ; absorption ; wheat ; Bengal gram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheatchapati, wheat+Bengal gram (80∶20 and 70∶30)chapatis and casein diets, at 10 percent protein level, were fed to rats for 12 weeks to study the effect on calcium utilization. The supplementation of Bengal gram to wheat diets significantly improved the calcium absorption. The urinary calcium excretion in wheat+Bengal gramchapati diets was significantly less than that of wheatchapati diet fed group. The in vivo45Ca absorption (CPM/100 μl serum) was also less in wheatchapati group as compared to wheat+Bengal gramchapati diets fed groups. The supplementation of legumes to cereals improved the calcium utilization and it may be concluded that there will be no risk of occurance of protein induced hypercalciuria.
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    URL: http://dx.doi.org/10.1007/BF02196469
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    Control of Mn status and infection rate by genotype of both host and pathogen in the wheat take-all interaction (1990)
    Springer
    Plant and soil 123 (1990), S. 267-275 
    Details
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; genotypes ; interaction ; manganese ; oxidation ; take-all ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Take-all is a world-wide root-rotting disease of cereals. The causal organism of take-all of wheat is the soil-borne fungus Gaeumannomyces graminis var tritici (Ggt). No resistance to take-all, worthy of inclusion in a plant breeding programme, has been discovered in wheat but the severity of take-all is increased in host plants whose tissues are deficient for manganese (Mn). Take-all of wheat will be decreased by all techniques which lift Mn concentrations in shoots and roots of Mn-deficient hosts to adequate levels. Wheat seedlings were grown in a Mn-deficient calcareous sand in small pots and inoculated with four field isolates of Ggt. Infection by three virulent isolates was increased under conditions which were Mn deficient for the wheat host but infection by a weakly virulent isolate, already low, was further decreased. Only the three virulent isolates caused visible oxidation of Mn in vitro. The sensitivity of Ggt isolates to manganous ions in vitro did not explain the extent of infection they caused on wheat hosts. In a similar experiment four Australian wheat genotypes were grown in the same Mn-deficient calcareous sand and inoculated with one virulent isolate of Ggt. Two genotypes were inefficient at taking up manganese and were very susceptible to take-all, one was very efficient at taking up manganese and was resistant to take-all, and the fourth genotype was intermediate for both characters. All genotypes were equally resistant under Mn-adequate conditions.
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    Role of bacterial cyanide production in differential reaction of plant cultivars to deleterious rhizosphere pseudomonads (1991)
    Springer
    Plant and soil 133 (1991), S. 93-100 
    Details
    ISSN: 1573-5036
    Keywords: cultivars ; growth inhibition ; hydrogen cyanide ; lettuce ; Pseudomonas fluorescens ; volatile metabolites ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lettuce and wheat cultivars, differing in reaction to root inoculation with plant growth-inhibitory bacteria, were tested for sensitivity to (i) gaseous metabolites produced by deleterious, cyanogenic isolates of Pseudomonas fluorescens, and to (ii) pure cyanide. Reactions were read as shoot and/or root elongation after exposure of seedlings to the volatiles in vitro. Lettuce cultivar Salad Bowl was significantly less sensitive than cv. Montana, both to bacterial volatiles and to pure cyanide, and a similar difference between these cultivars was also obtained in greenhouse experiments where bacteria were inoculated directly on the roots. Cultivar differences were, however, not recorded, either in vitro or in the greenhouse, when the bacteria were grown on a medium which did not support cyanide production. In wheat, a difference in sensitivity to bacteria-produced volatiles was recorded between two cultivars (Drabant and Besso) differing in reaction to bacterial inoculation, but in contrast to lettuce cultivars, the wheat cultivars tested did not react differentially to pure cyanide. The results suggest that in lettuce differential sensitivity to cyanide is one factor behind cultivar differences in reaction to the bacteria tested, even though bacterial metabolites other than cyanide may contribute to the plant growth inhibition recorded. In wheat, however, differential cultivar responses to these bacteria could not be shown to be related to cyanide.
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    Effects of rainfall on the use of foliar analysis for diagnosing boron toxicity in field-grown wheat (1992)
    Springer
    Plant and soil 140 (1992), S. 311-314 
    Details
    ISSN: 1573-5036
    Keywords: boron toxicity ; diagnosis ; foliar analysis ; mineral nutrition ; nutrient leaching ; rain ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of rain on foliage elemental composition, especially B, was assessed using samples of wheat collected at three harvests from a field trial conducted in soil containing excessive levels of B. Moderate rainfall substantially decreased both the B concentration and content of whole shoots and young leaves. The change in B concentration due to rain suggests that foliar analysis is unreliable for diagnosing B toxicity. For the other elements examined (Ca, Cu, Mg, Mn, Mg, P, S, Zn), rainfall had little effect.
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    The root activity of cereals and peas when grown in pure stands and mixtures (1992)
    Springer
    Plant and soil 142 (1992), S. 281-285 
    Details
    ISSN: 1573-5036
    Keywords: barley ; mixed-cropping ; peas ; root activity ; rooting-depth ; tracers ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lithium was used as a non-radioactive tracer to investigate the root activity of two cereals (wheat and barley), and of two contrasting cultivars of pea (leafy and semi-leafless), both in pure stands and in mixtures. The mixtures included combinations of each cereal with each pea cultivar in single rows, alternative rows and cross-drilled. Total lithium uptake (mg m-2) was higher for wheat than for barley, and higher for semi-leafless pea than for leafy peas. Growing cereals with peas reduced the total lithium uptake by peas, compared with pure stands, especially in alternate-row mixtures. Growing peas with cereals only reduced the total Li uptake by cereals when they were cross-drilled. The Li uptake by wheat, barley and peas generally decreased with soil depth in a similar manner; however, semi-leafless peas absorbed proportionately more Li from close to the soil surface than did leafy peas. Both pea cultivars absorbed more Li at 10–20 cm depth when grown in intimate mixtures with cereals, compared with less intimate mixtures or pure stands. The potential of lithium as a non-radioactive tracer in mixed-cropping studies is briefly discussed.
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    Empirical models to approximate calcium and magnesium ameliorative effects and genetic differences in aluminium tolerance in wheat (1992)
    Springer
    Plant and soil 144 (1992), S. 281-287 
    Details
    ISSN: 1573-5036
    Keywords: aluminium ; aluminium tolerance ; calcium ; magnesium ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The activities of inorganic, monomeric aluminium (Al) species in the root environment are important in the toxicity of Al to plant roots, which may be ameliorated by increased activities of basic cations. Additionally, it has been suggested that electro-chemical processes in walls of root cells play a role in Al tolerance. Empirical models were proposed to accomodate genetic and calcium (Ca) and magnesium (Mg) ameliorative effects on Al toxicity. The models were tested using data from a solution culture study (with ionic strength 1.6 to 8.6 mM) in which wheat (Triticum aestivum L.) cvv. Warigal (Al-sensitive) and Waalt (Al-tolerant) were grown for 28 d at 0, 10 and 20 μM Al, in factorial combination with 200, 400, 800 and 1600 μM Ca and 100, 200, 400 and 800 μM Mg. There was a poor relationship between relative total dry mass (TDM) (calculated as a percentage of the average TDM of each cultivar in the absence of added Al) and the activity of Al3+ or the sum of the activities of the monomeric Al species in solution. A model based on the ratios of activities of cations in solution, taking valency into consideration, was more successful, accounting for ca 85% of the observed variation in relative TDM. There were no systematic variations between observed values and those estimated by the model.
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    Extractable sulphate and organic sulphur in soils and their availability to plants (1994)
    Springer
    Plant and soil 164 (1994), S. 243-250 
    Details
    ISSN: 1573-5036
    Keywords: organic sulphur ; soil testing ; sulphate ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ten soils collected from the major arable areas in Britain were used to assess the availability of soil sulphur (S) to spring wheat in a pot experiment. Soils were extracted with various reagents and the extractable inorganic SO4-S and total soluble S(SO4-S plus a fraction of organic S) were determined using ion chromatography (IC) or inductively-coupled plasma atomic emission spectrometry (ICP-AES), respectively. Water, 0.016 M KH2PO4, 0.01 M CaCl2 and 0.01 M Ca(H2PO4)2 extracted similar amounts of SO4-S, as measured by IC, which were consistently smaller than the total extractable S as measured by ICP-AES. The amounts of organic S extracted varied widely between different extractants, with 0.5 M NaHCO3 (pH 8.5) giving the largest amounts and 0.01 M CaCl2 the least. Organic S accounted for approximately 30–60% of total S extracted with 0.016 M KH2PO4 and the organic C:S ratios in this extract varied typically between 50 and 70. The concentrations of this S fraction decreased in all soils without added S after two months growth of spring wheat, indicating a release of organic S through mineralisation. All methods tested except 0.5 M NaHCO3-ICP-AES produced satisfactory results in the regression with plant dry matter response and S uptake in the pot experiment. In general, 0.016 M KH2PO4 appeared to be the best extractant and this extraction followed by ICP-AES determination was considered to be a good method to standardise on.
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    Soil conduciveness to take-all of wheat: Influence of the nitrogen fertilizers on the structure of populations of fluorescent pseudomonads (1992)
    Springer
    Plant and soil 145 (1992), S. 29-36 
    Details
    ISSN: 1573-5036
    Keywords: ammonium ; cyanides ; Gaeumannomyces graminis var tritici ; nitrate ; nitrogen fertilizer ; pseudomonads ; soil conduciveness ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a field cropped with wheat, a high and low level of soil conduciveness to take-all were induced by applying a nitrogen fertilizer with either calcium nitrate or ammonium sulphate. From these two soils, two representative populations of fluorescent pseudomonads were tested for their in situ behaviour. Take-all index and root dry weight were assessed on plants cropped in soils infested with Gaeumannomyces graminis var tritici (Ggt) and each bacterized with one of the isolates of fluorescent pseudomonads. The bacteria tested can be split into three groups: antagonists which reduce take-all, deleterious isolates which aggravate the disease and neutral without evident effect on the disease. The predominance of antagonistic fluorescent pseudomonads in the NH4-treated soil and the predominance of deleterious ones in the NO3-treated soil was confirmed after statistical analysis. The microbial impact on take-all must be more considered as the resulting effect of divergent activities of both rhizobacteria types than the only consequences of the presence of antagonistic pseudomonads. All the high cyanogenic pseudomonads were antagonists in situ and were more numerous in the NH4-treated soil than in the NO3-treated soil.
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    Relationships between take-all, soil conduciveness to the disease, populations of fluorescent pseudomonads and nitrogen fertilizers (1992)
    Springer
    Plant and soil 145 (1992), S. 17-27 
    Details
    ISSN: 1573-5036
    Keywords: ammonium ; Gaeumannomyces graminis var tritici ; nitrate ; nitrogen fertilizers ; fluorescent pseudomonads ; root system ; soil conduciveness ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Take-all of wheat, caused by Gaeumannomyces graminis var tritici (Ggt), is reduced by ammoniacal fertilizers as compared to nitrate sources. This influence of nitrogen on the disease is only observed on nodal roots at flowering. But soil conduciveness to take-all, as measured in a soil bioassay, is modified earlier. Forty days after nitrogen application at early tillering, the NH4-treated soil became less conducive than the NO3-treated one. When nitrogen applications are done at sowing and at tillering, differences in disease propagation between the two soils are enhanced. Results from four years of experimentation show that when the level of natural soil inoculum is high, disease severity is reduced by ammonium, showing an effect on the parasitic phase of Ggt. At a low level of natural inoculum the effect of the source of nitrogen is mainly observed on the percent of infected plants, indicating that the saprophytic and preparasitic phases are affected. Rhizospheric bacterial populations increase from sowing to tillering, but differences on take-all conduciveness after tillering are not correlated with differences in the amounts of aerobic bacteria or fluorescent pseudomonads isolated from soils treated with different sources of nitrogen. Qualitative changes in fluorescent Pseudomonas spp. populations, like in vitro antagonism, are more likely to explain differences in soil conduciveness to take-all than are quantitative changes in this group. Nevertheless, the introduction of Ggt in a cropped soil leads to a greater increase in fluorescent pseudomonads populations than in total aerobic bacteria. The delay between reducing soil conduciveness and reducing disease in the field with ammonium nitrogen fertilization, the qualitative change of fluorescent pseudomonads populations and the role of necroses in rhizobacteria multiplication, provide information leading to our representation of a dynamic model based on the differentiation of the wheat root system into seminal and nodal roots.
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    Effect of various metal ions on growth of two wheat lines known to differ in aluminium tolerance (1993)
    Springer
    Plant and soil 155-156 (1993), S. 489-492 
    Details
    ISSN: 1573-5036
    Keywords: aluminium ; analog ; boron ; copper ; gallium ; iron ; lanthanum ; manganese ; scandium ; tolerance ; Triticum aestivum ; toxicity ; wheat ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of aluminium (Al), manganese (Mn), zinc (Zn), copper (Cu), boron (B), iron (Fe), gallium (Ga), scandium (Sc) and lanthanum (La) on growth of an Al-tolerant and an Al-sensitive line of wheat (Triticum aestivum L.) were measured in solution culture. The concentrations of nutrients in the basal nutrient solution were (μM) 500 Ca, 100 Mg, 300 K, 600 N (150 NH4, 450 NO3), 600 SO4, 2.5 P, 3 B, 2.5 Fe, 0.5 Zn, 0.5 Mn, 0.1 Cu at a pH of 4.7. The major solution nutrient concentrations were maintained at the nominal concentration with monitoring, frequent additions and weekly renewal. Differentiation in yield between the Al-tolerant and Al-sensitive line only occurred in the presence of Al indicating that, in the long term, none of the other metals tested could be used as an analog for Al. The visual symptoms in the roots of Cu toxicity (in both lines) and Al toxicity (in the sensitive line) were similar. The solution concentration (μM) at which yield of the roots of the tolerant line was reduced by 50% was, in order of increasing tolerance, Cu 0.5, Sc 1.1, La 7.1, Ga 8.6, Al 15, Zn 19, Fe 84, B 490 and Mn 600.
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    URL: http://dx.doi.org/10.1007/BF00025090
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    What is the significance of herbicide-nutrient interactions on wheat? (1993)
    Springer
    Plant and soil 155-156 (1993), S. 529-532 
    Details
    ISSN: 1573-5036
    Keywords: chlorsulfuron ; mineral nutrition ; sulfonylurea ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sulfonylurea herbicides have been found to decrease the uptake and utilization of some nutrients by wheat. This paper reviews the effects of sulfonylureas on nutrient uptake, proposes physiological mechanisms which might explain the effects; and examines the agronomic implications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025100
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    Modelling nutrient responses in the field (1993)
    Springer
    Plant and soil 155-156 (1993), S. 57-66 
    Details
    ISSN: 1573-5036
    Keywords: nitrogen ; wheat ; simulation ; yield-response curve
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Models of the yield responses of crops to applied nutrients are a recent addition to the methods available for making fertilizer recommendations. They have a place in integrating nutrient information with information on other factors which affect yield and its response to added nutrients. This review deals with nitrogen models classified into three groups: those which predict yield-response curves based on empirical factors; those which simulate the yield response from complex simulation models of many processes regulating crop growth and the soil environment; and those which aim to simulate yield and selected processes based on simplified functional relationships which apply to a target region or industry. Three case studies representing the three classes of model are drawn from research on dryland wheat in different parts of Australia. They show examples in which models provide information which is unobtainable from experimental procedures and which provide information useful to farmers in making decisions about fertilizers. Suggestions are made for future developments in crop-nutrient modelling including further comparisons of models, linkage of models with tissue tests, modelling co-limiting nutrients, deciding on the appropriate level of detail within a model and the need for methods for calibrating and testing models on attributes other than yield alone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024984
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  • 81
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    Modifications of the carbon and nitrogen allocations in the plant (Triticum aestivum L.) soil system in response to increased atmospheric CO2 concentration (1993)
    Springer
    Plant and soil 157 (1993), S. 215-225 
    Details
    ISSN: 1573-5036
    Keywords: carbon partitioning ; CO2 enrichment ; nitrogen mineralization ; nitrogen partitioning ; rhizosphere ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The aim of this work was to examine the response of wheat plants to a doubling of the atmospheric CO2 concentration on: (1) carbon and nitrogen partitioning in the plant; (2) carbon release by the roots; and (3) the subsequent N uptake by the plants. The experiment was performed in controlled laboratory conditions by exposing fast-growing spring wheat plants, during 28 days, to a 14CO2 concentration of 350 or 700 μL L−1 at two levels of soil nitrogen fertilization. Doubling CO2 availability increased total plant production by 34% for both N treatment. In the N-fertilized soil, the CO2 enrichment resulted in an increase in dry mass production of 41% in the shoots and 23% in the roots; without N fertilization this figure was 33% and 37%, respectively. In the N-fertilized soil, the CO2 increase enhanced the total N uptake by 14% and lowered the N concentration in the shoots by 23%. The N concentration in the roots was unchanged. In the N-fertilized soil, doubling CO2 availability increased N uptake by 32% but did not change the N concentrations, in either shoots or roots. The CO2 enrichment increased total root-derived carbon by 12% with N fertilization, and by 24% without N fertilization. Between 85 and 90% of the total root derived-14C came from respiration, leaving only 10 to 15% in the soil as organic 14C. However, when total root-derived 14C was expressed as a function of root dry weight, these differences were only slightly significant. Thus, it appears that the enhanced carbon release from the living roots in response to increased atmospheric CO2, is not due to a modification of the activity of the roots, but is a result of the increased size of the root system. The increase of root dry mass also resulted in a stimulation of the soil N mineralization related to the doubling atmospheric CO2 concentration. The discussion is focused on the interactions between the carbon and nitrogen allocation, especially to the root system, and the implications for the acquisition of nutrients by plants in response to CO2 increase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011050
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    A review of phosphorus efficiency in wheat (1992)
    Springer
    Plant and soil 146 (1992), S. 163-168 
    Details
    ISSN: 1573-5036
    Keywords: genetic variability ; NIR ; ploidy ; phosphorus ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract More efficient utilization of phosphorus by wheat plants is needed to extend the useful life of the phosphate reserves in the world, to reduce the cost of producing crops, and to improve the value of the grain and the straw produced. In this paper definitions of efficient use of phosphorus by wheat are reviewed, genotypic variation in phosphorus efficiency is reported, some consequences of breeding for greater efficiency are discussed, and ways to select more efficient genotypes are suggested.
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    URL: http://dx.doi.org/10.1007/BF00012009
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  • 83
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    Biofumigation: Isothiocyanates released frombrassica roots inhibit growth of the take-all fungus (1994)
    Springer
    Plant and soil 162 (1994), S. 107-112 
    Details
    ISSN: 1573-5036
    Keywords: canola ; glucosinolates ; Indian mustard ; take-all ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The presence of root tissue of the brassicas canola and Indian mustard inhibited growth of pure cultures of the fungal pathogen which causes take-all of wheat [Gaeumannomyces graminis (Sacc.) Arx and Oliver var.tritici, abbreviated as Ggt]. Ggt growth was generally inhibited more in the presence of Indian mustard roots than canola roots. Dried irradiated roots were consistently effective in reducing Ggt growth, but growth inhibition by young live roots and macerated roots was not consistent. The inhibitory compound(s) were shown to be volatile because the symmetry of Ggt growth was not affected by the proximity of theBrassica tissue. Volatile breakdown products from maceratedBrassica roots were identified using a gas chromatograph-mass spectrometer. The major compounds found were isothiocyanates (ITCs). Canola roots released mostly methyl ITC and Indian mustard roots released mostly phenylethyl ITC. Low concentrations of these and related compounds inhibited growth of Ggt in pure culture when supplied as the vapour of pure chemicals in concentrations within the range expected during breakdown ofBrassica roots in soil.
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    URL: http://dx.doi.org/10.1007/BF01416095
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    3Hthymidine incorporation of rhizosphere bacteria influenced by plant N-status (1994)
    Springer
    Plant and soil 162 (1994), S. 113-116 
    Details
    ISSN: 1573-5036
    Keywords: ammonium ; nitrate ; split-roots ; root exudate ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of plant-root N-status on bacterial growth in the rhizosphere was studied with 5-week-old wheat plants grown in soil with low N content obtained by mixing 9:1 gravel:sandy loam. As a consequence of N limitation, significant increase in3Hthymidine (Tdr) incorporation rate occured 3 days after addition of 30 mM ammonium compared to controls without ammonium. Plants were grown with split-roots to separate the effect of soil N from effect of plant root derived organic matter-N on bacterial activity. The increase in nitrate concentration from 10 mM to 30 mM at one part of the root system led to significant increased3HT dr incorporation in the rhizosphere at the other part of root system after 4 days showing that the composition of root exudates became more favourable for bacterial growth when plants were fertilized with the higher level of nitrate.
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    URL: http://dx.doi.org/10.1007/BF01416096
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    Growth, biomass allocation and water use efficiency of two wheat cultivars in a mediterranean environment; a pot experiment under field conditions (1994)
    Springer
    Plant and soil 162 (1994), S. 241-247 
    Details
    ISSN: 1573-5036
    Keywords: biomass allocation ; carbon isotope discrimination ; growth ; water use efficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plants of two bread wheat cultivars,Triticum aestivum L. ‘Katya Al’ and ‘Mexipak 65’, were grown in pots during the crop season in the field in NW Syria, a region with a Mediterranean climate. The experiment involved two treatments. Control plants were well-watered throughout the experiment (watering to 0.22 g water g−1 dry soil). In the second treatment, water was withheld from the plants until soil water content had decreased to 0.10 g water g−1 dry soil, the level that was maintained thereafter. Water use was measured by weighing the pots, and growth by destructive sampling. Growth of Katya and Mexipak was similar. Mexipak had a lower (though not significant atp〈0.05) plant water use efficiency (WUEp) in both treatments due to higher rates of water loss. On a leaf area basis differences in water use were especially high since Mexipak had a smaller total leaf area. In spite of a smaller investment in photosynthesizing area, Mexipak achieved similar growth as Katya. Carbon isotope discrimination and organic nitrogen concentration (both higher for Mexipak) suggest that Mexipak accomplished higher mean net photosynthetic rates with a higher mean leaf diffusive conductance, higher intercellular carbon dioxide partial pressure, and possibly a greater investment in the photosynthetic apparatus compared to Katya. Differences in carbon isotope discrimination suggest a larger difference in average photosynthetic WUE (net photosynthesis/transpiration) than in plant WUE. This could indicate that loss of carbon in respiration was greater in Katya. Gas exchange measurements on the youngest fully expanded leaves showed only minor differences between the cultivars. It is hypothesized that Mexipak, with a smaller total leaf area, is able to maintain high leaf conductance and photosynthesis for a longer period of time during the day or during the life span of leaves.
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    URL: http://dx.doi.org/10.1007/BF01347711
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    Genotype-environment interaction and correlation between vegetative and grain production measures of potassium use-efficiency in wheat (T. aestivum L.) grown under potassium stress (1993)
    Springer
    Plant and soil 151 (1993), S. 39-44 
    Details
    ISSN: 1573-5036
    Keywords: efficiency ratio ; harvest index ; potassium ; Triticum aestivum ; utilization index ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nutrient use-efficiency (NUE) is commonly measured in relation to vegetative growth without regard for economic productivity of crops whose valued product is reproductive. Although vegetative measures can be useful, particularly in forage crops, their validity in the quantification of NUE in grain crops is questionable. This study was undertaken primarily to examine the relationship between vegetative and economic potassium use-efficiency (KUE) in several wheat (T. aestivum) genotypes under conditions of potassium stress. Genotype environment interaction for vegetative KUE was also examined. Vegetative KUE was assessed as shoot fresh weight, efficiency ratio (VKER) and utilization index (VKUI) whereas economic KUE was evaluated as grain weight, total weight and economic efficiency ratio (EKER). Significant genotype-environment interactions for shoot weight, VKER and VKUI were observed. In some instances interaction was associated with crossovers between genotypes indicating that it can affect selection. Correlations between vegetative and economic measures of KUE were generally nonsignificant, but negative and significant for shoot weight of three-week-old plants and grain weight. It appears that if genotypes differ considerably for harvest index and it is not positively correlated with total biomass, vegetative measures of KUE are unreliable as indicators of economic KUE. Therefore, economic rather than vegetative measures should be used to evaluate KUE in crops whose valued product is reproductive. ei]{gnH}{fnMarschner} ei]{gnH.}{fnLambers}
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    URL: http://dx.doi.org/10.1007/BF00010784
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    Non-structural carbohydrate: Analysis by near infrared reflectance spectroscopy and its importance as an indicator of plant growth (1993)
    Springer
    Plant and soil 155-156 (1993), S. 243-246 
    Details
    ISSN: 1573-5036
    Keywords: fructans ; NIR ; nitrogen ; non-structural carbohydrates ; rice ; starch ; stress ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant shoot samples are frequently analysed to assess if crops require additional nitrogen or mineral elements to maintain satisfactory growth. If plant growth is limited by temperature, water stress, disease, lodging or a mineral deficiency, non-structural carbohydrates (NSC) may be accumulated in, or depleted from, tissues especially those in the lower stems. Plant testing laboratories do not routinely analyse NSC to assist in the identification of plant stress probably because skilled technicians and time are required for the wet chemical determination. In this paper we report that routine determination of NSC is possible using near-infrared reflectance spectroscopy; the errors of determination are comparable with traditional chemical methods. The concentration of NSC in the shoots of rice grown in south eastern Australia ranges from 1.6 to 22.8%, as starch. In the shoots of wheat grown in eastern Australia the range is from 2.4 to 35.2%, as fructans. In both crops the NSC content is highly inversely correlated with the shoot nitrogen content. Based on data from commercial wheat and rice crops we suggest that the ratio between nitrogen and NSC can be used to identify crops in which growth has been limited by a stress other than nitrogen and so are unlikely to show the predicted response to an application of nitrogen fertilizer.
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    URL: http://dx.doi.org/10.1007/BF00025029
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    A new approach to quantify the utilization of non-exchangeable soil potassium by plants (1993)
    Springer
    Plant and soil 149 (1993), S. 235-243 
    Details
    ISSN: 1573-5036
    Keywords: De ; exchangeable K ; K diffusion ; K release ; K utilization ; model calculation ; non-exchangeable K ; root hairs ; sugar beet ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To predict the contribution of soil K fractions of different mobility to K supply of plants, the kinetics of K release from soil was related to the kinetics of K uptake of young sugar beet and wheat plants. For this purpose K release rates from soil were measured by continuously percolating samples of a luvisol with 0.01 M CaCl2 solution and effective diffusion coefficients, De, were determined. Two soil K fractions of different mobility were obtained. De values of the more mobile ‘exchangeable K’ and the less mobile ‘non-exchangeable’ K fraction were found to be 58.9 × 10−9 and 8.2 × 10−9 cm2 s−1, respectively. In a pot experiment, sugar beet and wheat plants were grown, for 15 days and both root growth and K uptake were measured. K uptake kinetics of both crops was determined in a separate experiment using flowing solution culture. To integrate these data quantitatively, the simulation model of Claassen et al. (1986) was applied. Results show that calculated total K uptake agreed closely with real K uptake of the plants. On this basis, 64 and 79% of the K taken up by wheat and sugar beet plants was derived from the rapidly released ‘exchangeable’ and 21–36% from the less mobile ‘non-exchangeable’ soil K fraction.
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    URL: http://dx.doi.org/10.1007/BF00016614
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    The 2,4-D-induced wheat para-nodules are modified lateral roots with structure enhanced by rhizobial inoculation (1994)
    Springer
    Plant and soil 159 (1994), S. 121-129 
    Details
    ISSN: 1573-5036
    Keywords: 2,4-dichlorophenoxyacetate ; para-nodule ; Rhizobium trifolii ; structure ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nodular outgrowths (para-nodules or p-nodules) on the roots of wheat (Triticum aestivum L.) cv. Miskle seedlings were induced by treatment with 0.3 and 0.6mg L−1 2,4-dichlorophenoxyacetate (2,4-D). When co-inoculated with Rhizobium trifolii strain ATCC 14480, more p-nodules were formed at these levels and p-nodulation occured at 0.1 mg L−1 indicating that inoculation enhances 2,4-D-induced p-nodulation. Similar to lateral roots, the p-nodules arose from the pericycle opposite the phloem tissues and were free from the cortical cells of the parental root at all stages of development. Structurally, the p-nodules exhibited tissue differentiation. They possessed a highly organized central vascular cylinder connected to that of the parent root, an endodermis, a cap, and an apical and lateral meristems. P-nodules formed by 2,4-D treatment alone were irregularly lobed due to uncoordinated activity of the apical meristem, while those in the combined 2,4-D and inoculation treatment were more globose. The results of the present study indicate that the 2,4-D-induced wheat p-nodules are modified lateral roots, the structure of which is enhanced by rhizobial inoculation.
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    Regulation of NH4 + uptake in wheat plants: Effect of root ammonium concentration and amino acids (1993)
    Springer
    Plant and soil 151 (1993), S. 211-218 
    Details
    ISSN: 1573-5036
    Keywords: amides ; free ammonium ; MSX ; net ammonium uptake inhibition ; total free amino acids ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract When N deficient 10-day-old wheat plants were supplied with 1.5 mol m−3 NH4 +, net NH4 + uptake rapidly decreased during the first 6h, while root free-NH4 + and free amino acids concentration increased. However, after 24 h the NH4 + uptake rate increased again, as the internal NH4 + concentration decreased. When plants were pretreated during 40 h with different external NH4 + concentrations, net uptake measured on 1.0 mol m−3 NH4 + decreased with the increasing ion concentration during the pretreatment. This decrement coincided with both root free-NH4 + and total free amino acids levels. When N-starved and NH4 + fed plants were treated during 0, 3 or 6 h with 1.0 mol m−3 NH4 + in the presence of 1.0 mol m−3 MSX2, net uptake (measured without MSX) decreased with the length of the inhibitor treatment. In both groups, MSX significantly increased root free-NH4 + concentration, while the level of total free amino acids was only increased in N-starved plants. When N-starved plants were externally supplied with 1.0 mol m−3 of different amino acids or amides, net NH4 + uptake was only strongly inhibited in the presence of glutamine or asparagine. It is concluded that rapid changes in the concentration of certain amino acids during NH4 + nutrition might regulate the ion absorption, though at high endogenous levels of free NH4 + net uptake could be suppressed independently of the root concentration of free amino acids.
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    Interaction of salinity and enhanced ammonium and potassium nutrition in wheat (1993)
    Springer
    Plant and soil 154 (1993), S. 133-137 
    Details
    ISSN: 1573-5036
    Keywords: ammonium nutrition ; potassium nutrition ; salinity ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat (Triticum aestivum L.) was grown to maturity in a pot experiment in a calcareous silty sand soil. N was applied at two levels as granulated N-P fertilizers, amended or not with nitrification inhibitors (1% and 5% DCD, 1% N-serve). Potassium as KCl was given at three levels of application. P was applied at a uniform rate. Two levels of salinity were obtained by using the soil as such (EC= 0.3 mmho/cm) and by adding NaCl to the same soil (EC=2.4 mmho/cm). 1% DCD and 1% N-serve treatments gave significantly higher wheat grain yields and N-uptake than the other ones. Nitrate content of leachates indicated a prevalent nitrate nutrition in the treatment without nitrification inhibitors. The 5% DCD treatment showed a yield depression. In the lower N level treatments, a significant yield increase, generated by 1% DCD and N-serve was found in the salinized soil as compared to the non-saline soil. Soil salinity reduced N-uptake when nitrification inhibitors were not present. In treatments having the inhibitors, N-uptake was equal or greater in the salinized than in the non saline soil. An enhanced ammonium nutrition increased the P uptake.
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    Understanding zinc reactions in vertisols to predict zinc responses by wheat (1993)
    Springer
    Plant and soil 155-156 (1993), S. 247-250 
    Details
    ISSN: 1573-5036
    Keywords: rate of Zn desorption ; Vertisols ; wheat ; Zn buffer power ; Zn desorption power ; Zn intensity ; Zn quantity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Since Zn availability to plants growing in a soil is governed by quantity, intensity, buffer power, rate of Zn desorption and diffusion, an improved understanding of a number of these factors in Vertisols would facilitate a more reliable prediction of crop requirements for Zn. The DTPA-extractable Zn, a quantity factor, together with initial Zn desorption rate coefficients, accounted for 80% of the variation in relative dry matter yield of wheat grown to anthesis. In combination with these factors, desorption (buffer) power explained 92% of the variation in Zn concentration in the young mature leaf blade (YMB) of wheat. Thus, the combination of the quantity, rate of Zn desorption and buffer power better predict growth responses of wheat to applied Zn in Vertisols than the commonly-used single extraction with DTPA alone (quantity), which provides only a static measure of Zn availability.
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    URL: http://dx.doi.org/10.1007/BF00025030
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    The control of boron accumulation by two genotypes of wheat (1993)
    Springer
    Plant and soil 155-156 (1993), S. 305-308 
    Details
    ISSN: 1573-5036
    Keywords: boron ; wheat ; Triticum aestivum L. ; uptake ; genotypic variation ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The mechanism of boron (B) uptake in wheat was studied using two genotypes with known differences in their ability to accumulate B. Influx and efflux of B was measured in the roots of intact 21 d old plants. Roots grown in 15 μM B, when transferred to solutions containing 1mM B showed a rapid increase in B content for up to 60 min, after which no further increase was evident up to 4 h. No genotypic difference in B influx was apparent over these time periods. Roots grown in 1mM B for 7 d and then rinsed in B-free solutions quickly lost most of B that they contained within 1 hour; little further efflux was observed over the following three hours. As with the influx, no genotypic difference in B flux was evident. It is suggested that the lack of genotypic difference in the short-term B fluxes could be due to a masking effect of extracellular B bound in the cell walls of the roots.
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    Distribution of potassium in the soil profile of a sandplain soil under pasture species (1993)
    Springer
    Plant and soil 155-156 (1993), S. 407-410 
    Details
    ISSN: 1573-5036
    Keywords: L. cosentinii ; nutrient recycling ; potassium ; subterranean clover ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of pasture species and pasture/crop rotations on the fate of K fertilizer in the soil profile of a sandplain soil was investigated. Results for Lupinus cosentinii, subterranean clover and a subterranean clover/wheat rotation are presented. Potassium was applied as KCl at six rates up to 150 kg K ha-1 for three years; bicarbonate-extractable K was measured at five depths in the profile (0–100 cm) for four years. The net change in available K in the top 100 cm of the profile (kg ha-1) was calculated. There was a gradual increase in K down the profile under all species with fertilizer application. The increase was largest for L. cosentinii, which also appeared to redistribute K from below 100 cm to the soil surface. The K residual value on this soil type was higher than expected with most of the fertilizer applied over three years being retained in the top 100 cm.
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    Development of an ELISA for estimation of the copper nutritional status of wheat and cotton (1993)
    Springer
    Plant and soil 155-156 (1993), S. 453-456 
    Details
    ISSN: 1573-5036
    Keywords: copper deficiency ; cotton ; ELISA ; Gossypium hirsutum ; immunoassay ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Studies were carried out with hydroponically grown wheat and cotton to develop the Cu-requiring protein phenolase as a biomarker of Cu nutrient status. Isozymes of phenolase whose levels were reduced by Cu deficiency were identified by Western blots. A competitive enzyme-linked, immunosorbent assay (ELISA) was developed that could detect as little as 25 ng of phenolase. The ELISA revealed that Cu-sufficient cotton leaves had about 4-fold more phenolase antigen than did Cu-sufficient wheat leaves. In both species, the level of phenolase was reduced by 2- to 5-fold in leaves of Cu-deficient plants. Because the immunoassay for phenolase protein is rapid, inexpensive, and can be carried out with small amounts of leaf material, it has potential as a tool for assessment of the Cu status of crop plants.
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    The 2,4-D-induced wheat para-nodules are modified lateral roots with structure enhanced by rhizobial inoculation (1993)
    Springer
    Plant and soil 157 (1993), S. 121-129 
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    ISSN: 1573-5036
    Keywords: 2,4-dichlorophenoxyacetate ; para-nodule ; Rhizobium trifolii ; structure ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nodular outgrowths (para-nodules or p-nodules) on the roots of wheat (Triticum aestivum L.) cv. Miskle seedlings were induced by treatment with 0.3 and 0.6 mg L-1, 2,4-dichlorophenoxyacetate (2,4-D). When co-inoculated with Rhizobium trifolii strain ATCC 14480, more p-nodules were formed at these levels and p-nodulation occured at 0.1 mg L-1 indicating that inoculation enhances 2,4-D-induced p-nodulation. Similar to lateral roots, the p-nodules arose from the pericycle opposite the phloem tissues and were free from the cortical cells of the parental root at all stages of development. Structurally, the p-nodules exhibited tissue differentiation. They possessed a highly organized central vascular cylinder connected to that of the parent root, an endodermis, a cap, and an apical and lateral meristems. P-nodules formed by 2,4-D treatment alone were irregularly lobed due to uncoordinated activity of the apical meristem, while those in the combined 2,4-D and inoculation treatment were more globose. The results of the present study indicate that the 2,4-D-induced wheat p-nodules are modified lateral roots, the structure of which is enhanced by rhizobial inoculation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00038755
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  • 97
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    Electronic Resource
    A comparison of minirhizotron techniques for estimating root length density in soils of different bulk densities (1993)
    Springer
    Plant and soil 157 (1993), S. 239-245 
    Details
    ISSN: 1573-5036
    Keywords: bean ; bulk density ; compaction ; minirhizotron ; root image analysis ; root quantification ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Flexible- and rigid-walled minirhizotron techniques were compared for estimating root length density of 14- to 28-day-old Pinto bean (Phaseolus vulgaris L.) and spring whet (Triticum aestivum L.) plants in soil boxes under controlled environment conditions at three soil bulk densities (1.3, 1.5 and 1.7 g cm−3). The flexible-tube system consisted of bicycle inner tubes inflated inside augered access holes and removed only when measurements were taken. Rigid tubes were constructed of extruded polybutyrate plastic. In both cases tubes were oriented horizontally. Despite similar root densities for wheat and beans based on measurements obtained from soil cores, root densities estimated from both types of minirhizotron were higher in bean than in wheat in uncompacted soil. Estimates of root density by the flexible tube minirhizotron were more closely correlated with soil core image analysis estimates than were those by the rigid minirhizotron system. At high soil bulk density, rigid tube measurements consistently overestimated actual rooting density of both wheat and bean. The relationship between estimated and actual rooting densities in the case of flexible tube measurements was not significantly influenced by soil bulk density. These findings were consistent with the theory that preferential root growth is induced by gaps at the soil-observation tube interface, inherent in the rigid tube technique, and was accentuated under conditions of high soil strength.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011052
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  • 98
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    Electronic Resource
    The completion of cell proliferation and growth in wheat radicle (1994)
    Springer
    Plant and soil 167 (1994), S. 43-49 
    Details
    ISSN: 1573-5036
    Keywords: DNA contents ; DNA synthesis ; growth ; proliferation ; radicle ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The pattern of proliferation and growth of cortical and central metaxylem cells in a radicle and the transitional zone of a wheat embryo was studied during the final stages of embryogenesis. Cell divisions finished nearer the root tip in the central metaxylem than was the case in the cortex. After divisions ceased the cells of both tissues maintained the ability to synthesize DNA and the cells began DNA endoreduplication. The maximal levels of endoreduplication were 4C and 8C in cortical and central metaxylem cells, respectively. As a result of nonsimultaneous cessation of divisions, the metaxylem cells were two or three times longer than cortical cells. The proportion of cells with the maximal DNA content was smaller in the transitional zone than in the radicle. During the final embryonal stages cell growth rate was decreased. It was established that the transition of cells to DNA synthesis was inhibited in all sites of the radicle during the completion of embryogenesis. The cell growth was topped in proximal sites of the radicle. In the division zone the cells which had already begun DNA synthesis were able to complete it and divided. Cell growth stopped simultaneously with completion of proliferation in this zone.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01587596
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  • 99
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    Electronic Resource
    Variation for root traits and their genetic control in spring wheat (1991)
    Springer
    Euphytica 59 (1991), S. 1-8 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; root ; combining ability ; correlation ; dry matter ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat root characters which influence vital plant processes have scarcely been explored for their genetic control. This study was conducted to i) examine the diversity of root traits and associated shoot traits in spring wheat (Triticum aestivum L.) cultivars; ii) study the nature of genetic control of selected traits; and iii) examine associations among root and shoot traits. Three experiments were conducted in the greenhouse with plants grown in the vermiculite medium in clear plastic tubes. In the first experiment, 42 spring wheat cultivars were grown for three weeks and measurements were taken on root length, leaf length, root number, leaf number, root dry weight, and top dry weight. In the second study, 15F1's originating from a partial diallel mating of six cultivars along with the parents were evaluated for 4 weeks. The data on root length, leaf length, and root number were subjected to diallel analysis according to Griffing's method 4, fixed model. A third experiment consisted of studying 2 F2 populations with 141 plants per population. In the first study the 42 cultivars exhibited a wide range of variation for all six traits. Diallel analysis revealed significant effects of both general combining ability (GCA) and specific combining ability (SCA) for root length, leaf length, and root number. Parents with high GCA estimates were identified. Significant positive correlation coefficients were found among root and shoot traits. Analysis of F2's for root length indicated quantitative nature of inheritance of root length.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025355
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  • 100
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    Electronic Resource
    Growth of wheat leaf rust colonies in susceptible and partially resistant spring wheats (1990)
    Springer
    Euphytica 45 (1990), S. 71-80 
    Details
    ISSN: 1573-5060
    Keywords: wheat ; Triticum aestivum ; wheat leaf rust ; Puccinia recondita f.sp. tritici ; partial resistance ; histology ; growth curve
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The average size of wheat leaf rust colonies, measured using epifluorescence microscopy was significantly larger in the highly susceptible genotype Morocco than in the susceptible genotype Kaspar and the partially resistant genotypes Westphal 12A, Akabozu and BH 1146. This was already so three days after inoculation. Colony growth in partially resistant genotypes was continuously retarded compared to colonies in the highly susceptible genotype Morocco. No evidence was found for an initial inhibition of the growth of colonies in partially resistant genotypes. In partially resistant genotypes formation of uredial beds and sporulating areas started at a smaller colony size than in susceptible genotypes. Wheat leaf rust colonies in primary leaves of all genotypes studied were much larger than colonies in flag leaves measured at the same number of days after inoculation. Growth and sporulation of not intertwined colonies was not influenced by either a high or a low number of neighbouring colonies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00032152
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