Publication Date:
1986-08-29
Description:
Epstein-Barr virus (EBV), a ubiquitous human herpesvirus, has the ability to transform human B lymphocytes. No other cell type has been experimentally transformed by EBV, either by intact virions or naked viral DNA and subgenomic fragments. Two immortalized human T-lymphoblastoid cell lines have now been established by transfecting cord blood lymphocytes with purified B95-8 viral DNA enclosed in fusogenic Sendai virus envelopes (RSVE) and then exposing the cells to EBV from a P3HR-1 cell subclone. One of these lines, which has been fully characterized, is termed HBD-1. This line is positive for EBV DNA and expresses surface OKT11, OKT4, and Tac receptors, but not M-1, mu immunoglobulin chains, EBV receptors, or B-1 surface markers. The cells contain fully rearranged T-cell receptor genes and germline immunoglobulin genes. The karyotype of the cells is normal, they do not require interleukin-2 for growth, and do not contain human T-lymphotropic virus type I. However, the HBD-1 cells contain incomplete EBV genomes and express several EBV-determined antigens, including the early antigen type D, membrane antigens, but not EBV-determined nuclear antigen (EBNA). This association of the EBV genome with permanently growing hematopoietic cells of non B-cell lineage should prove useful in studies on the mechanism of EBV-mediated cell transformation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Stevenson, M -- Volsky, B -- Hedenskog, M -- Volsky, D J -- CA33386/CA/NCI NIH HHS/ -- CA37465/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1986 Aug 29;233(4767):980-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3016899" target="_blank"〉PubMed〈/a〉
Keywords:
Cell Line
;
Cell Survival
;
DNA, Viral/*genetics
;
Deltaretrovirus/genetics
;
Herpesvirus 4, Human/*genetics
;
Humans
;
Nucleic Acid Hybridization
;
T-Lymphocytes/*microbiology/physiology
;
*Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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