ALBERT

Notes: Land systems are patterns of landscape recognised on aerial photographs and mapped to define areas of similar terrain with a similar environment. Breaks of slope often form the major boundaries both between and within land systems. They can be usefully mapped from aerial photographs even if their significance can only be deduced after ground investigation. Many of the characteristics of land systems can be appreciated from aerial photographs and measurements made on the photographs can improve their quantitative description.

Notes: A method of slotted templet triangulation is described in which the authors have used photographs printed on a stable base as the templets. The advantages, disadvantages and possibilities of the method are discussed.

Notes: After a brief review of the history of the orthophoto mapping process and its methods, the paper describes the automatic production of orthophotographs in the BS-Stereomat. Technical details, especially of the electronics of the instrument, are given. The range of applications of an orthophoto is discussed, as well as the method's limitations.

Notes: This paper discusses a simple method of computing independent strips under certain reasonable approximations. It is shown that, if the method is regarded as if it were computation by polynomials, the order of the polynomial is decided by the nature of the control provided and not by the number of controlled models. This conclusion should throw some light on the vexed (and perennial) problem of the choice of orders for polynomials.

Notes: SYNOPSIS. Microstome →macrostome transformation in Tetrahymena vorax was induced by suspending microstomes in a transforming principle, stomatin, released by a potential prey, T. pyriformis. It was found that 70–90% of the microstomes formed macrostomes within 7 hours following suspension in this transforming principle. Macrostome formation occurred by the process of oral replacement. This process involved resorption of the microstome oral apparatus and its replacement with a larger (macrostome) one, which arose from an anarchic field that formed behind the resorbing oral area. Ninety-five percent of those microstomes which were destined to form macrostomes were in some stage of oral replacement 195 minutes after their suspension in stomatin. Several commercially produced products were tested over a wide range of concentrations to determine their ability to act as an inducer of macrostomes. Only 2, Trypticase and Bactocasitone, had any activity, and it was too small to be considered really effective. An attempt was also made to destroy the activity of stomatin by using enzymes. RNAse was effective but only in very high concentrations, so it was suggested that this activity might be related to the destruction of RNA within the transforming cell and not related to hydrolysis of stomatin. None of the other enzymes tested had any effect in reducing the activity of stomatin.

Notes: SYNOPSIS. A developmental sequence is proposed for the haplosporidan Minchinia nelsoni Haskin, Stauber and Mackin, 1966, based on study of oyster infections over the past 5 years in Chesapeake Bay. Uninucleate stages develop by nuclear division into multinucleate plasmodia which proliferate in the tissues by plasmotomy. Relatively small plasmodia containing what are considered to be gametic nuclei originate by unequal plasmotomy of large plasmodia. These have been interpreted to aggregate and fuse to form large plasmodia which contain prozygotes. Pairing and fusion of nuclei occur within each plasmodium to produce zygote nuclei (synkaryons) which undergo division, possibly meiotic, to form sporonts. Sporoblasts differentiate into spores with the development of spore walls and opercula. Cystoid plasmodia develop during times of unfavorable conditions. An anomalous but common sequence involving sexuality and mitosis is described, and the occurrence of various life cycle stages within the host thruout the year is discussed.

Notes: SYNOPSIS. Many of the sub-pellicular and infraciliary structures in protozoa have proved difficult to study with standard thin-sectioning technics. When these structures are viewed in isolated and fragmented form, many of the thin-sectioning difficulties are circumvented. Langmuir-trough isolation followed by critical-point drying, as well as thin sectioning, were used in this study to determine the patterns of sub-pellicular microtubules and fibrils interconnecting kinetosomes of membranelles and cirri of Euplotes eurystomus. The fibrillar network in the bases of these ciliary organelles is presented in some detail and apparent variations in pattern are noted. Functional aspects of some of the structures are discussed. With special preparation nearly whole Euplotes may be obtained for study in the electron microscope. Fused cilia were frequently obtained and their ultrastructure was studied.

Notes: SYNOPSIS. A technic is described for the quantitative assay of paramylum content of euglenoid flagellates. The method relies on the alkaline solubility of paramylum followed by treatment with the anthrone reagent. The intensity of the color developed by paramylum is about 14% greater than that developed by an equivalent amount of glucose. The method is sensitive down to about 10 μg.

Notes: SYNOPSIS. The development of three 8-liter and four 12-liter cultures of the photosynthetic dinoflagellate Gonyaulax monilata was followed for 4 months. Weekly estimates were made of population levels of this chain-forming flagellate, along with incidence of cells in chains and toxicity to fish. Guppies (Lebistes reticulatus) were used to assay toxicity. Populations reached a peak when cultures were 3–5 weeks old, declined during weeks 6–10, and tended to stabilize thereafter thru the 17th (final week). The percentage of cells in chains was related to the slope of the population curve; rapidly increasing populations had the highest proportion of long chains, suggesting that incidence of chains is an index of the growth phase in G. monilata. Peak toxicity was not reached until culture populations had been steadily declining for a month, indicating that most toxin is released by autolysis. The reproducibility of culture population and toxicity levels recommend the methods used for future studies.

Notes: SYNOPSIS. The pigments synthesized by Astasia ocellata include α- and ε-carotene, 4-keto-β-carotene (echinenone), and 4,4′-diketo-β-carotene (canthaxanthin); 4-keto-α-carotene, accounting for about half the pigment in the cells, was tentatively identified; a strongly adsorbed keto-carotenoid, accounting for 25% of the pigments and bearing some similarities to astacin, polytomaxanthin and phoenicoxanthin, was also found.

Notes: SYNOPSIS. Blepharisma intermedium was cultured axenically. The organisms were freed of contaminating microorganisms by serial washing in a sterile salt solution. The major nutrients in the various media were: Freshly killed or lyophilized and autoclaved bacteria (Pseudomonas ovalis), yeast extract prepared from Fleischmann's baking yeast or Fleischmann's yeast autolysate, lettuce infusion or stigmasterol, 6 B-vitamins, and phosphate buffer at 2 times 10−3 M. The cultures were kept in the dark at 25 C. Altho the 1st division after transfer into fresh media was delayed, B. intermedium divided in approximately 32 hours thereafter in most of these media.

Notes: SYNOPSIS. The effects of temperatures of 12–18 C on cell division and oral primordium development were investigated in cultures of synchronized Tetrahymena pyriformis GL-C. If exposures to 12 or 15 C were initiated prior to a “transition point,” long delays of cell division were generated. After this transition point, cell division could no longer be substantially delayed by exposure to low temperature. The time of the transition point was somewhat earlier with 15 C than with 12 C treatments. At temperatures higher than 15 C long delays of cell division were not generated regardless of time of treatment.The effects of low temperature on oral morphogenesis were strongly dependent on the stage which was affected. (i) The further development of cells initially in the “anarchic field” stage (stage 1) was immediately blocked at both 12 and 15 C. (ii) Cells initially in the stages of incipient membranelle differentiation (stages 2 and 3) continued to develop at both 12 and 15 C, and formed oral primordia in which all 3 membranelles were clearly differentiated (stage 4). The subsequent progress of these stage 4 primordia depended on the temperature: at 12 C virtually all were resorbed (and cell division was blocked); at 15 C only about 1/3 were resorbed, while the remaining 2/3 completed their development (with the concomitant completion of cell division). (iii) Cells initially in intermediate stages of membranelle differentiation (early stage 4) developed to some extent at 12 C, and then underwent resorpton of oral primordia and blockage of cell division; at 15 C such cells completed their development and division normally. (iv) Cells in which the membranelles and undulating membrane were complete or nearly so (stage 5 and very late stage 4) at the time of the beginning of the cold treatment subsequently finished their development and went thru cell division, even at temperatures as low as 5 C.These results indicate that in addition to a “stabilization point” which occurs shortly before the completion of membranelle development, there is an earlier change in the primordium at the time of the onset of membranelle development, which renders development much less sensitive to direct interference by low temperature.

Notes: SYNOPSIS. Excysted sporozoites of Eimeria meleagrimitis, E. necatrix, E. acervulina, and E. gallopavonis were inoculated into monolayer cell cultures of bovine, ovine, porcine, and human kidney. E. meleagrimitis developed only in bovine embryonic kidney. Mature schizonts were found in the 11th, 16th, and 20th serial passages, but only immature schizonts were in the 4th and 6th passages. E. necatrix developed to mature schizonts in the 3rd, 4th, 6th, 11th, 16th, and 20th passages of bovine kidney and also to immature schizonts in the 175th and 189th passages of PK-15 (cell line porcine kidney). Schizonts, however, did not develop in the 140th and 145th passages of CCI-33 (cloned PK-15). Neither E. meleagrimitis nor E. necatrix developed in the primary, 1st or 2nd passages of bovine embryonic kidney, primary porcine kidney, 45th and 52nd passages of a human embryonic kidney cell line, or in the primary, 5th and 18th passages of ovine kidney. Eimeria acervulina and E. gallopavonis did not develop in any of the cultures. E. meleagrimitis and E. necatrix probably completed only one asexual generation in culture. The structure of mature schizonts of both species differed greatly from those in the natural host. Schizonts of E. meleagrimitis present at 48 hours were small (13–18 by 12–14 μ) and contained only 12–28 merozoites that were 3.2–3.8 μ long. At 48 hours, E. necatrix schizonts were 15–18 μ in diameter or less and contained only 15–20 merozoites (2.0–3.5 μ long); at 96 hours they were 50–70 by 10–35μ and contained either hundreds of small merozoites (2.0–3.5 μ long) or a lesser number of larger merozoites (9–11 μ).

Notes: SYNOPSIS. In heavily endemic collecting sites in Panamá and Colón Provinces, Republic of Panama, 14.7% of Ameiva ameiva and 8.5% of Basiliscus basiliscus were injected with Besnoitia darlingi. Single infected specimens of A. leptophrys and A. festiva were also taken, these being new host species records for this parasite. Infections were found only in the older lizards.Initially, virulence of the lizard parasites for white mice was low but increased with successive mouse passages. Concomitantly, the cyst-forming capacity of the strain diminished with successive mouse passages. No relation between initial virulence of the lizard parasites for mice and subsequent virulence after 16 or 17 mouse passages was recorded.The original description of B. panamensis (a synonym of B. darlingi) is emended on the basis of extensive material to include cyst diameters of 200–500 μ; also, the liver, mesentery, and tunica propria of the testis occasionally contain cysts. Cysts are frequently macroscopic and on the surface of organs so that they can be seen on casual inspection. B. sauriana Garnham, 1966 is a synonym of B. darlingi.

Notes: SYNOPSIS. An electron microscope study of microgametocytes and macrogametes of Eimeria nieschulzi Dieben, 1924 revealed that they lie within vacuoles bounded by a host unit membrane. The vacuole surrounding the microgametocyte contains granular material. The vacuole around the macrogamete is narrower and contains vesicles and membranes. Micropores were seen on the surface of the plasma membrane of microgametocytes and macrogametes. Microtubules were seen in macrogametes. Young microgametocytes and macrogametes have a similar cytoplasmic matrix, mitochondria and nuclei. Glycogen granules apparently develop around vacuoles in both microgametocytes and macrogametes. Glycogen granules were also seen along the margins of parallel bundles of fibers in microgametocytes. As nuclei of the microgametocyte divide, they move to the periphery of the parasite. Three basal bodies, each with 9 fibers in triplet form, develop in association with each nucleus. Microgametes have 2 free flagella and a central short, attached flagellum. Basal granules lie along the outer fibers of the central flagellum. Each microgamete has an elongate mitochondrion in close contact with the nucleus. In macrogametes wall-forming bodies develop in lacunae in the cytoplasm. Smaller dark bodies with areas of low density were also seen. Wall-forming bodies and dark bodies move to the periphery of mature macrogametes.

Notes: SYNOPSIS. In the nearly mature macrogametes of Eimeria auburnensis, the cell membrane is a unit membrane, with underlying and overlying osmiophilic layers usually present. Cup-shaped micropores were occasionally seen. Smaller, V-shaped invaginations were also found in considerable numbers at the surface. At the deepest point, these invaginations were bounded only by a unit membrane. Immediately adjacent to this point, vesicles with homogenous electron-pale contents bounded by a similar unit membrane, were frequently seen. Pinocytosis evidently occurs at the site of these invaginations. Numerous folds of the host cell membrane bordering the vacuole in which the parasite lay extended about 0.1–0.7 μ into the vacuole. These “intravacuolar folds” varied in depth and number in different specimens. In some, the majority of folds had apparently become disconnected from the host cell membrane. A highly developed smooth endoplasmic reticulum occurred in the adjacent host cell cytoplasm. The intravacuolar folds may assist in transfer of nutrients, including membrane material, from the host cell to the parasite. The evidence indicates that in this species of Eimeria nutrients are taken into the parasite primarily as fluids by pinocytosis and possibly other processes.

Notes: SYNOPSIS. Sexual reproduction of up to 50% of the cells from 9 cultures of Stentor coeruleus was observed repeatedly for several months. Photomicrographs were taken of mating pairs, and a number of new observations were made, including the ability of well fed cells (containing food vacuoles) to conjugate, the ability of a high percentage of cells (approaching 50%) in a culture to mate at one time, a more detailed description of the region of cell attachment as viewed from the frontal fields of the partner cells, and the ability of one partner to twist off and leave its oral area attached to the other member of the pair.

Notes: SYNOPSIS. Comparison of RNA molecules between certain protozoa using the technic of nucleic acid hybridisation revealed that there are complementary sequences for ribosomal RNA molecules in the genomes of such cells. Furthermore the genes for ribosomal RNA have been conserved during evolution in this group of organisms. On the other hand, RNA molecules from these protozoa which can be considered to be “messengers” show little in the way of sequence relationships. By utilising the technic of hybridisation it was found that Oxytricha can compete effectively against Paramecium ribosomal RNA for Tetrahymena DNA but the ribosomal RNA sequences of the latter could not compete completely against Paramecium ribosomal RNA for Oxytricha DNA. The result is interpreted to show that different ribosomal sequences were hybridising with each of the DNA samples from Tetrahymena and Oxytricha. A general interpretation of this result in terms of ribosome evolution is presented.

Notes: SYNOPSIS. Strains of Tetrahymena pyriformis, including amicronucleate strain GL and representatives of 9 syngens, have been examined to determine the patterns whereby cortical features vary with numbers of ciliary meridians. The characteristics scored were the positions of the contractile vacuole pores (CVP's), the extent of the area within which CVP's develop, the incidence of supernumerary CVP's, and the number of postoral meridians. Intrasyngenic comparisons were possible in 6 syngens and permitted an assessment of intrasyngenic variation for these characteristics. Only the CVP positions appear to be reasonably constant within syngens in the strains examined. On the basis of this criterion the syngens can be arrayed in an approximate order of 1, 3, 7, 6, 8, 9, 2, 5, GL and 4; the angle formed between the central axis, the stomatogenic meridian and the CVP's is most acute in syngen 1.

Notes: SYNOPSIS. The bionomics of Labyrinthula Cienkowski and the validity of the family Labyrinthulidae Haeckel are reviewed. The structure, physiology (including nutrition), locomotion, ecology, and possible pathogenicity for eel-grass (Zostera marina L.) are discussed. The uniqueness of its gliding motility along slime tracks, lack of phagotrophy, and poorly understood congregation tendencies emphasize the present taxonomic isolation of the group. Further interest attaches to some species because of their steroid requirements.

Notes: SYNOPSIS. Sporozoites of L. simondi were maintained in a viable state for 7 months in liquid nitrogen. Comparison of parasite development initiated with fresh and frozen sporozoites showed a delay in development of each stage studied. Comparisons of prepatency, first elongate gametocytes, peak density of round and elongate forms, anemia and disappearance of megaloschizonts were made. In each phase there was a delay of 2–3 days in ducks infected with frozen sporozoites.

Notes: SYNOPSIS. Ten strains of Acanthamoeba from freshwater habitats were isolated in clonal cultures. Studies were made of trophic structure, nuclear division, cyst structure, some aspects of cytochemistry, and other characteristics. One strain was identified as A. castellanii (Douglas, 1930), one as A. astronyxis (Ray and Hayes, 1954), and 8 as A. polyphaga (Puschkarew, 1913). Strains of Acanthamoeba isolated by other workers were also examined comparatively.The pattern of nuclear division in all strains resembled that in metazoan cells, with the exception that centrioles were never found. Trophic amoebae had a PAS-positive surface outline. Cyst walls were strongly PAS-positive and also gave a positive test for cellulose with zinc chloroiodide.The genus Acanthamoeba Volkonsky, 1931 is re-defined, being distinguished from Hartmannella Alexeieff, 1912, emend. Volkonsky, chiefly by the formation of tapering, hyaline pseudopods (acanthopodia) and by a cyst made up of an ectocyst and a polyhedral or stellate endocyst, with excystment by removal of opercula. Other characteristics found in all strains include a distinctive food cup, the presence of many small refractile globules in the cytoplasm of trophic amoebae, and a cyst wall containing cellulose. The degree of spindle convergence, employed by Volkonsky as a generic criterion, was unusable.Differential diagnoses based principally on cyst structure are offered for A. castellanii, A. astronyxis, and A. polyphaga. The strain previously called Mayorella palestinensis Reich, 1933 is a distinct species of Acanthamoeba.

Notes: SYNOPSIS. Parauronema virginianum n. g., n. sp., a marine hymenostome ciliate is described from the Virginia coast. Structural studies were made on specimens treated with the Chatton-Lwoff silver impregnation technic and on animals observed with the phase microscope. Particular attention was given to the buccal ciliature and its importance to generic assignment in the order Hymenostomatida.

Notes: SYNOPSIS. Large numbers of sessile ciliates were successfully collected in plastic petri dishes with tight-fitting lids, transported in the water-filled dishes without disturbance. Each species within the transparent dishes was identified with a dissecting microscope and the position on the dish surface of each sessile individual was located and recorded on graph paper for further quantitative comparisons. This method was used for numerous experiments on the ecology and behavior of sessile ciliates and their responses to toxins.

Notes: SYNOPSIS. Structure and morphogenesis, and cytochemical data on Cochlodinium heterolobatum, a new species of unarmored dinoflagellate, were derived from living and fixed material from culture. C. heterolobatum is characterized by the torsion of the girdle which descends in a left-hand spiral 1.8 turns; the sulcus having a torsion of 0.8 turn; a sulcus loop in the epicone; a tongue-shaped lobe in the right hypocone; nucleus in the epicone; and a stigma in the left epicone. Trichocysts and behavior of the nucleus during typical and atypical divisions are described in cells from cultures of different ages. A small form with the specific characters was found. Intracellular bacteria were seen and their growth followed in individuals from cultures of different ages. A possible relationship between those bacteria and the accumulation of metabolites inside old cells is discussed.

Notes: SYNOPSIS. Species of sessile Stentor, Vorticella and colonial peritrichs were collected in boxtype plastic petri dishes. Thru a hole in the wall of the dish, various concentrations of mercuric chloride, a standard bactericidal agent, were introduced. Following a 3-hour exposure to the toxicant, the death of the sessile ciliates followed a set pattern with a clearcut toxicity threshold of 0.1–0.5 parts per million with an LD 50 of about 0.25 ppm. Furthermore, the sessile ciliates were not dislodged when the natural pond water in the dish was poured out and replaced with water from other sources. Various responses were elicited by 4-hour exposure to 4 changes of environment. By this procedure, the protozoan community was subjected to new sets of conditions. Such a technic could serve as a method of detecting and assessing water pollution.

Notes: SYNOPSIS. From characteristics of binary fission, conjugation, size and number of micronuclei, body size and incidence of giantism, a Blepharisma isolate hitherto called B. undulans is classified as B. dawsoni sp. nov. Binary fission in B. wardsi differs from fission in B. dawsoni in that the strand connecting the macronuclear nodes is severed; in B. dawsoni the strand persists.

Notes: SYNOPSIS. Experiments with mixtures of latex-tagged cultures of amoebae of strains, species and genera of the Dictyosteliaceae indicate little specificity in engulfment. This is in sharp contrast to the decided specificity for compatibility in completion of morphogenesis in the same organisms. Lack of specificity in engulfment is considered additional evidence favoring engulfment as a normal feeding activity rather than a process associated with syngamy in the cellular slime molds.

Notes: SYNOPSIS. Eimeria colchici sp. n. is described from English pheasants Phasianus colchicus. It has cocysts measuring 19–33.5 (27.4) by 13–21 (16.7) μ, having both micropyle and polar granule. The large 1st and 2nd generation schizonts occur in the glands of the small intestine, 3rd generation schizonts occur deep in the glands of the ceca, and gametocytes occur in the epithelial cells of the ceca. The prepatent period is 6 days. In young pheasants the parasite causes coccidiosis characterized by formation of white cores in the ceca. Mortality is high in experimental infections. Zoalene (0.0125%) in the feed almost completely suppressed the infection; Amprolium (0.0125%) also gave good control, but sulfaquinoxaline (0.0125%) was not so effective. Sulfaquinoxaline, Saquadil and Sulfamezathine in the drinking water controlled mortality, but treatment had to be applied early to avoid weight losses.

Notes: SYNOPSIS. Monolayer primary and secondary cultures of embryonic bovine kidney, spleen, intestinal and testicle cells, and secondary cultures of embryonic bovine thymus, maintained in lactalbumin hydrolysate, Earle's balanced salt solution and ovine serum were observed for a maximum of 21 days after inoculation of E. bovis sporozoites. The sporozoites entered the cells in all of these cultures but underwent development only in primary cultures of kidney and intestinal cells and in secondary cultures of kidney, spleen, thymus, intestinal, and testicle cells. In acellular media, the sporozoites retained motility no longer than 21 hr. In the cell cultures, free motile sporozoites were seen for as long as 18 days after inoculation. Sporozoites entered cells anterior end first; the process of penetration required a few seconds to about a minute. Sporozoites were also observed leaving host cells. Intracellular sporozoites were first seen 3 min after inoculation; they were observed at various intervals up to 18 days after inoculation. In transformation of sporozoites into trophozoites a marked change in size and appearance of the nucleus took place before the change in shape of the body occurred. Trophozoites were first found 7 days after inoculation, multinucleate schizonts after 8 days, and schizonts with merozoites after 14 days. Schizonts containing merozoites were seen only in kidney, spleen, and thymus cells. The mature schizonts were smaller and represented a much lower proportion of the total number than in comparable stages of infections in calves. Schizonts with many nuclei occurred in intestinal cells; the most advanced stage seen in testicle cells was the binucleate schizont. Nuclear and cytoplasmic changes were observed in the infected cells.

Notes: SYNOPSIS. Peritoneal macrophages from hamsters were monolayered on coverslips in Leighton tubes. Twenty-four hours later these were transferred to a perfusion chamber. Leptomonads were added with fresh medium and the infection process observed with the aid of phase contrast. In the perfusion chamber free-swimming leptomonads attached to the macrophage by the tip of their flagella. Shortly after this initial attachment the macrophage extended a narrow pseudopodium around the flagellum which eventually reached and enveloped the body of the parasite. Upon complete envelopment the pseudopod containing the leptomonad was retracted into the central body of the macrophage. When first seen in the granular endoplasm of the macrophage, most of the leptomonads appeared to be surrounded by vacuoles. In most cases these vacuoles disappeared in a few minutes making it difficult to distinguish the parasite from the host cell cytoplasm.Leptomonads also were added directly to Leighton tube cultures, and the coverslips with the adherent macrophages and parasites were removed, fixed and stained periodically during the infection process. In these preparations most of the parasites were in clumps in the vicinity of macrophages. Details of the ingestion of the clumps could not be seen, but occasionally a single organism was seen with its flagellum and part of its body enclosed by an extended pseudopod. Most of the intracellular leptomonads were in large vacuoles. Forms intermediate between elongate leptomonads and LD bodies were surrounded by smaller vacuole-like spaces. The halo-like vacuoles most frequently seen around LD bodies may have been fixation artifacts. Under favorable conditions the leptomonads transformed to LD bodies in 1–4 hours, but it was 48 hours before a population increase could be found.

Notes: SYNOPSIS. Coccidia-free beagle puppies were experimentally infected with a cloned culture of Isospora rivolta oocysts. The endogenous stages were found in the posterior 1/2 of the small intestine, and rarely in the cecum and colon. Maximum numbers of all stages occurred just anterior to the ileocecal valve. Endogenous stages were found in the distal third of the villi, predominantly parasitizing subepithelial cells of the lamina propria; however, stages were occasionally present in epithelial cells. The number of asexual generations could not be determined from their structure, but evidence based on oocyst production suggested that there were at least 2 asexual generations. The schizonts were 17–24 by 12–25 μ and contained 4–24 merozoites, the most common number being 4 or 8. Schizonts with mature merozoites were found as early as 72 hr, but were present in maximum numbers at 96 hr. Merozoites had slender curved bodies and were 10.5–13.4 by 2.3–3.0 μ. Mature gamonts were found by 144 hr. Mature microgametocytes were 13.4 by 8.7 μ and contained 50–70 microgametes. Microgametes had slightly curved tapering bodies (5.8–6.4 by 0.6 μ) with 2 posteriorly directed flagella 11–14 μ long. Mature macrogametes had reticular cytoplasm and a uniformly large nucleus and nucleolus.The prepatent period was 142–146 hr. The patent period was 13–23 days with an average of 19 days.

Notes: SYNOPSIS. Rabbit antisera to strains of different mating types of Chlamydomonas moewusii and to one strain of C. eugametos were tested against strains of C. reinhardti, C. eugametos, and C. moewussi, and against strains of 2 varieties and 6 mutant types of C. moewusii. Technics of double diffusion, absorption, and immunoelectrophoresis revealed marked serological differences between the sexually incompatible, distinct genetic species C. moewusii and C. reinhardti. Less distinct serological differences were resolved between C. moewusii and the so-called “species”C. eugametos, which is sexually compatible with the former, thus reconfirming the conspecificity between the 2 strains as suggested by Gowans. Marked serological differences were noted between C. moewusii and 2 of its varieties (C. moewusii var. tenuichloris and C. moewusii var. rotunda) which constitute 2 additional genetic species because of sexual incompatibility between themselves and with C. moewusii. Wild types and certain mutants of C. moewusii were compared serologically and could be distinguished on this basis. Strains of different mating types as well as certain mutant strains (e.g., paralyzed flagella, flagella-less, twins and monsters) could be differentiated serologically altho differences were often very subtle. Some antigens were common to organisms tested.

Notes: SYNOPSIS. Optimal growth of the colorless flagellate, Astasia longa, in terms of generation time occurred at 25–30 C. Above and below this interval, generation time increased. In contrast, length of the lag phase was constant over a wide temperature range, 18–32 C. At 13 C dry weight of Astasia was 15% greater than at 28.5. Alcohol-ether soluble material accounted for 72% of the dry weight increase at 13, while RNA and protein contents of the cells were the same at both temperatures. Rates of synthesis of dry weight, RNA, protein and lipid per μl oxygen consumed were all reduced at 13 C; however, lipid synthesis was the least affected. Cell division in Astasia was synchronized repetitively by cold (13–15 C) and warm (28.5 C) temperature cycles. Under best conditions, cell division began immediately at the onset of the warm period. Cellular preparations for cytokinesis apparently were at least partially completed in the cold, and raising the temperature released a block to cell division.

Notes: SYNOPSIS. Isolated rumen protozoan species (Entodinium) were incubated in vitro with starch, antibiotics and Na214CO3. 14C-carbonate was incorporated into amino acids of the Entodinium protein. Arginine, aspartic acid, alanine, and glutamic acid had the highest specific activities. Carbonate was incorporated into the TCA-soluble fraction, into cell polysaccharides, and into ether and acetone extracts of TCA-precipitates.

Notes: SYNOPSIS. Light-dependent incorporation of acetate occurs in an obligate phototrophic strain of Euglena gracilis (strain L). Assimilation is into all major biochemical fractions. Acetate does not induce operation of the glyoxylate by-pass as it does in heterotrophic strains; neither does it stimulate oxygen consumption. Acetate will not replace CO2 in phototrophic growth. A number of carbon sources tested would not support growth in the dark, and glucose was not incorporated either in the light or the dark.

Notes: SYNOPSIS. The uptake, distribution and turnover of phosphorus have been studied for the culture form of Trypanosona cruzi. Following exchange reactions, phosphorus was accumulated at an approximate rate of 1.9 μg/108 trypanosomes/hour in Krebs-Ringerphosphate. Of 3.8 mg P/g trypanosomes (wet weight), 60% occurred in acid-soluble, 13% in phospholipid, 22% in nucleic acid, and 5% in phosphoprotein fractions. Acid-soluble and phosphoprotein fractions incorporated P32 more rapidly than phospholipid and nucleic acid fractions, the phosphoprotein fraction having the highest specific activity by 8 hours of incubation.

Notes: SYNOPSIS. Sulfaquinoxaline and ethopabate are 2 chemically distinct types of antagonist of PABA with anticoccidial action. Reversal experiments with PABA and synergism studies with pyrimethamine indicated that both compounds interfered with the PABA-folic acid metabolic sequence. Six pure strains of Eimeria brunetti responded differently to the 2 compounds. The strain most sensitive to ethopabate was one of 2 strains most resistant to sulfaquinoxaline. Conversely, the strain most sensitive to sulfaquinoxaline was unresponsive to the highest doses of ethopabate employed. Hypotheses to explain the differences in biological effects of the 2 compounds were considered.

Notes: SYNOPSIS. Stationary cultures of Ochromonas danica accumulated lipids as they aged. The bulk of the increase in fatty acids was in the unsaturates, particularly the polyunsaturates. The quantity of lipid peroxides (thiobarbituric acid-positive material) also increased with age. Aging was also associated with increase in sensitivity to inhibitory compounds. The implications of lipid accumulation for cell sensitivity are discussed.

Notes: SYNOPSIS. The size of the population of Naegleria gruberi at the time the amoebae are offered the opportunity to become flagellated is not a critical factor in the morphogenesis of this organism. Small populations (1-15 cells) readily become flagellated. Small populations (1-5 cells) washed several times also become flagellated. Clonal populations (25) have been established. All clones yield flagellates under the usual conditions. It is suggested that the physiological state of the amoebae is a factor in determining the number of cells that will undergo morphogenesis at any given time.

Notes: SYNOPSIS. Frame by frame analysis of cinephotomicrographs of the evacuation of the water excretory vesicle of Amoeba proteus shows that this organelle (commonly called the “contractile vacuole”) does not contract as the water is expelled. Instead, pressure exerted by the underlying endoplasmic “gel” pushes the vesicle against the plasmalemma, bulging the latter until a pore opens through it. The cell membrane and apposed vesicular membrane both rupture through the plasmalemmar pore; the fluid rushes out; and the vesicle collapses against and inverts into itself, but does not contract. There is therefore no systole of the vesicle due to any contraction of it. A survey of the literature shows that others who have studied the evacuation of these water excretory vesicles in amebas, suctorians, and ciliates have also described them as collapsing, not contracting. Recent electronmicrographs of a number of protozoa also indicate that the water excretory vesicle collapses, and does not contract as it evacuates its contents; and that there are no fibrils surrounding the organelle which might promote its contraction. We suggest that the term “contractile vacuole” be discarded as a descriptive and identifying term for such organelles; and that they be called “water expulsion” organelles. We further suggest that the term “vacuole,” which implies that the structure is empty, be replaced by the term veside, implying its function as a small container, and that the organelle be preferentially called the water expulsion vesicle. We also suggest that the terms “systole” and “diastole” be discarded and be replaced with enlargement and evacuation so that the erroneous implication of a contraction and relaxation cycle may be dispelled.The succeeding new water expulsion vesicle in Amoeba proteus seldom forms at the site of the old one, but usually forms at a new site in the sol anterior to the gel of the tail region, to which it is transferred prior to expulsion. A tentative suggestion of how this transfer may occur is offered.

Notes: SYNOPSIS. Adenosine triphosphate (ATP) with pyruvate, or adenosine diphosphate with phosphoenolpyruvate, favor the development of Plasmodium lophurae removed from its host erythrocytes and kept extracellularly in vitro. It seemed possible that the parasites might be deficient in enzymes of the glycolytic cycle concerned with the generation of ATP. The ATP content of duck erythrocytes infected with P. lophurae was lower than that of uninfected cells. Infected erythrocytes, however, had somewhat higher contents of both pyruvic kinase and phosphoglyceric kinase than did uninfected ones. Both of these enzymes could be found in the free parasites. Furthermore, the pyruvic kinase of the free parasites was inactivated by freezing and thawing, whereas that of the host erythrocyte was not affected. It will be necessary, therefore, to look further for the basis for the favorable effect of ATP with pyruvate on parasites developing extracellularly in vitro.

Notes: SYNOPSIS. Properties and cellular location of acid phosphatase in Trypanosoma gambiense were studied. Activity was found in both the sediment (32,000 ×g) and the supernatant of homogenates. Cenrifugation in 0.3 M sucrose showed activity principally in the lowspeed fraction (4,000 ×g). One min of sonication released most of this activity. Several phosphomonoesters were hydrolyzed at acid H's. Enzymatic activity was relatively specific for pyrophosphate and p-nitrophenylphosphate at pH 3.6. At pH 5.2, purine and pyimidine nucleotide 5′-triphosphates as well as adenosine di- and ono-5′-phosphates were hydrolyzed nonspecifically. Activity with yrophosphate at pH 3.6 had a temperature optimum of 60-70 C while that for adenosine 5′-triphosphate (pH 5.2) was 50 C. These ctivities of the sediment required no metal co-factors and were inibited by Fe++, inhibition at the lower pH being greater.Glucose 6-phosphate was hydrolyzed by the supernatant with maximum activity between pH 6.0 and 7.2 and a temperature optimum of 50 C. This pH range showed a broad plateau with 2 or 3 minor peaks. The hydrolysis of p-nitrophenylphosphate showed a similar pH curve. In glucose 6-phosphate hydrolysis, Mg++ was a required co-factor but could be replaced by Ni++ or Co++. Ammonium sulfate fractionation precipitated most of the supernatant activity between 50 and 75% saturation.A modified Gomori technic produced spherical deposits of PbS thruout the cytoplasm of the intact cell. With the electron microscope, Pb phosphate deposition was observed in membrane-bound vesicles (i.e., lysosomes) approximately 100-150 mμ in diameter. These organelles were common in the region of the reservoir at the base of the flagellum. Acid phosphatase activity specific for glucose 6-phosphate as substrate was localized within this basal pocket.

Notes: SYNOPSIS. Trypanosoma occidentalis sp. n., a hemoflagellate encountered in the blood plasma of 3 freshwater teleosts, Cottus gulosus, C. rhotheus (Family Cottidae) and Gasterosteus aculeatus (Family Gasterosteidae) from Washington State, is described and recorded. Morphologic and morphometric features are detailed, and comparisons are made with other trypanosomes reported from North American fishes and from related species of teleosts from Eurasia. The relationship of the geographic isolation of parasite, hosts, and potential vector in the determination of the new species is discussed.

Notes: SYNOPSIS. By means of the ninhydrin-Schiff method for proteins a diffuse reaction as well as one localized in granular inclusions can be shown in the cytoplasm of fibroblasts, epithelial cells, and macrophages in trypsin-dispersed chick liver cell cultures. Nuclei and nucleoli also take the specific stain. A progressive loss of cytoplasmic and nuclear staining occurs in the fibroblasts in cultures infected with a relatively pathogenic strain of T. vaginalis. A loss occurs in epithelial cells in advanced stages of degeneration, but in less damaged cells, while the diffuse reaction disappears, the number and staining intensity of the cytoplasmic inclusions remain unchanged or possibly may increase somewhat. The intensity of the diffuse reaction and the number and size of the characteristic inclusions increase in the active, parasite-free, experimental macrophages, but phagocytes with trichomonads closely applied to their external surfaces and those containing the flagellates within their cytoplasm typically retain only a few weak-staining inclusions.Similar distribution of alkaline and acid phosphatases occurs in preparations treated according to Gomori's and Burstone's methods, except that no nuclear staining is obtained with the latter. Activity of both enzymes is localized primarily in inclusions which are dispersed thruout the cytoplasm of fibroblasts and epithelial cells and tend to accumulate along the cell membranes and around the nuclei. In the course of infection with T. vaginalis there is a progressive loss of alkaline phosphatase from both cell types; however, the acid phosphatase activity increases. In the control macrophages both enzymes are localized in mostly rather large, rounded cytoplasmic inclusions. The number of such inclusions increases in the parasite-free experimental macrophages, but only a few weak-staining granules remainin phagocytes with engulfed trichomonads and in those whose external surfaces are in direct contact with the parasites. The loss of the inclusions is less apparent in macrophages containing degenerated flagellates than in the ones with healthy trichomonads, but regardless of the condition of the parasites, the highest enzymatic activity is found around them.ATPase and 5′-nucleotidase are localized in small granules dispersed thruout the cytoplasm of fibroblasts and epithelial cells. The granules tend to accumulate along the periphery of the cells and around the nuclei. A diffuse cytoplasmic reaction is present in preparations processed for 5′-nucleotidase. Nuclei and nucleoli give positive reactions for both enzymes. In the course of infection with trichomonads, activity of the 2 enzymes declines in both culture cell types. Control macrophages have diffuse cytoplasmic reaction for ATPase and 5′-nucleotidase and these enzymes are localized also in rounded cytoplasmic inclusions. Activity of both enzymes increases in the parasite-free experimental phagocytes, but little if any diffuse staining and only a few characteristic inclusions are left in macrophages with engulfed healthy trichomonads and in those whose external surfaces are invested with the flagellates.The ninhydrin-Schiff-positive inclusions found in the macrophages appear to be the same as some of those which have acid phosphatase activity and may well be identical with the glycolipoprotein bodies noted by us previously. On the grounds of their chemical constitution and behavior it seems likely that the inclusions are lysosomes.

Notes: SYNOPSIS. Chilomonas paramecium developed significant resistance to INH at concentrations up to 150 mg/100 ml. Once established, the resistant strains grew subnormally in drug-free medium and were more susceptible than the normal strain to sulfanilamide, but were much more resistant to PABA (10-20 mg/100 ml). In addition, INH-adapted strains were less responsive than the controls in PABA reversal of SA-inhibition. Strains adapted to sulfanilamide, sulfapyridine, and nicotinamide were less resistant to INH than the normal strain.

Notes: SYNOPSIS. The ultrastructure of the herbivorous amoeba Pelomyxapalustris was studied. Nuclear division is not understood in this amoeba, and evidence for the method of nuclear division was sought. This species typically has many spheroidal nuclei which are similar within a given cell. However, some amoebae from our collections differed from this common type in both the number and structure of their nuclei. This suggested stages associated with nuclear division. One current hypothesis of nuclear division in this organism is that of nuclear budding. Our evidence is more in accord with this method than with mitosis.The cytoplasm contained no mitochondria, Golgi bodies, contractile vacuoles or crystals. Most amoebae had 2 types of bacteria (bacteroids or endosymbionts) in their cytoplasm; a separate vesicle enclosed each of these. Characteristically, only 1 type of bacterium (Bn) surrounded the nucleus. Another type (B) was found elsewhere in the cytoplasm. Also in the cytoplasm were the following: food vacuoles enclosing various algae, relatively clear vacuoles and vesicles, glycogen, various electron-opaque particles, and occasional microtubules. The plasmalemma was smooth, lacking the external fringe which characterizes other large fresh-water amoebae.

Notes: This article discusses the problem of compiling a balanced set of national accounts at constant prices. The method adopted is based on earlier work on this subject by Burge and Geary. Commodity flows, which are uniquely deflatable, are expressed at constant prices and savings in constant prices is obtained by preserving a balanced set of equations in real terms. The deflation of the external account is discussed.A method is suggested for expressing the national income account in real terms and an “income gain” is deduced for each industrial sector which represents the difference between real income and real product in that sector. The sum of the income gains for the domestic sectors is zero.The constituents of the income/expenditure accounts of households, corporations and general government are expressed at constant prices by selecting suitable deflators in a consistent manner. The accounts in real terms are now unbalanced and are balanced again by inserting a balancing item which is shown to represent a gain to the sector arising from changes in the terms of trade between the sectors. This item is called an “expenditure gain”. The sum of the expenditure gains for the institutional sectors is zero.The system suggested can be extended to cover additional items in the accounts and thus a complete set of national accounts in real terms can be derived.

Notes: The interrelation between changes in the economic structure, i.e., industrial distribution of income and labor force, and the size distribution of income is studied in this paper in a case study of India (1951–1960).The change in the size distribution of income is the sum of changes due to (1) inter-sectoral factors and (2) intra-sectoral factors. The need for this distinction is emphasized by the result obtained for India, that 85% of the changes in the size distribution may be assigned to inter-sectoral factors, and only 15% to intra-sectoral factors. Since the inter-sectoral factors are significantly influenced by changes in the industrial distribution of income and labor force, our result points out a relation between economic growth and the size distribution which quite often is overlooked in studies of the size distribution.The results obtained in this paper support several cross-section results of Professor Kuznets. In particular some of these are: (a) inter-sectoral inequality in the economic structure widened with economic growth, (b) the inequality in the size distribution of India widened, (c) the level of inequality in India is higher than in any of the eight developed countries considered.

Notes: The technique of national income accounting is a part of what Hicks has termed “The Fixprice Method”. Deflation is an attempt to approximate a real economy to a fixprice economy. It is shown that if the propositions of macro-dynamics are to hold, this deflation cannot be done in accordance with the price structure prevailing at any particular historical time, but must use that given by the capital theory of value, viz., when returns to labour are equal to zero. For a labour-abundant developing economy this will correspond to prices based on opportunity cost principles.As an illustration, sectoral incomes on this basis have been calculated for the industrial sector of the Indian economy for the years 1951 to 1965.

Notes: This report summarizes the proceedings of a series of meetings called by the Conference on Research in Income and Wealth of the National Bureau of Economic Research in June of 1966. The major conclusions of the conference, as transmitted to the Statistical Office of the United Nations, were as follows: (1) The aim of integrating the various parts of the system of national accounts, including input-output and financial transactions, is to be welcomed. (2) The more recently developed parts of the system need considerably more work to reach the same level of clarity and usefulness which the national income and product accounts have acquired. (3) Some simplification of the proposed basic system should be considered, involving the identification of a minimum of information that should and could be provided by all countries. (4) In line with the conference's overriding interest in national accounts as an instrument for economic analysis and a means of more informed policy formation, the proposed system needs considerable strengthening in the field of income distribution.

Notes: Economists' use of the term “equality” in reference to a distribution of incomes has historically been in the sense of a consensus for some statistical characteristic(s) of the distribution rather than a firm concept of equality. Of course such a concept rests on appropriate welfare assumptions about income and its distribution, assumptions which, for the most part, have been left implicit (and unknown) in discussions of income equality in the literature.Our purpose in this paper is dual: first, we wish to discover an unambiguous, welfare-related equality measure. This we accomplish through suitable assumptions on a social welfare function. What is produced is an “index” of equality which describes the performance of a given distribution relative to the maximum welfare derivable from the total income it represents. The measure thus depends functionally on the welfare attributes of income, something which in reality we know little about.This impasse leads us to inquire into the sensitivity of the index over specifications of the welfare function, which is done by comparing equality ranks for the states of the United States for 1960 under various functional forms and among curves within a given form. As an interesting secondary issue, the performance of traditional equality measures is tested relative to the welfare-oriented index to discover implications about their welfare content.It is found that the equality index is, in certain ranges for the welfare function, insensitive to its specification. The findings lead directly to conclusions concerning traditional equality measures, their usefulness in correctly accounting for equality differences among alternative income distributions and, concomitantly, their implicit welfare inputs.

Notes: This paper presents the results of an investigation of the distribution of Yugoslavia's national income by social classes in 1938. The population in mid-1938 was apportioned among social classes as follows: proletariat 34.6 per cent, middle classes 59.2 per cent, bourgeoisie 5.3 per cent, 0.9 per cent unallocated. About three-quarters of the population was rural. The proletariat amounted to 5.2 million persons, of which 3 million were peasants living on dwarf holdings and 2.2 million were rural and urban wage earners. Unemployment in the non-agricultural sector was 10 per cent; if the agricultural sector is added, overall un- and under-employment amounted to 31 per cent. The bourgeoisie consisted of 0.8 million persons, of which two-fifths were rich peasants. Of the 9 million persons in the middle classes, 7 million were peasants with small and medium holdings. The remainder were mainly minor entrepreneurs in the non-agricultural sector. The proletariat accounted for 35 per cent of total population but only 18 per cent of aggregate income, whereas the bourgeoisie with 5 per cent of the population received 26 per cent of aggregate income. The distribution of income among the various groups of the non-agricultural population was more unequal than among the groups of the agricultural population. Estimates are preesented of the distribution of income by various types and sources, for agricultural and non-agricultural population, together with income per capita, average earnings per employed worker, labor productivity, and capital intensity, the last by industrial branches as well as social classes.

Notes: One of the most characteristic features of Japan's public sector is the predominant role of the Treasury system, which operates not only budgetary funds of the central government but also various other funds such as Postal Savings Funds and surplus funds of public corporations.Among the general account and 45 special accounts of the Treasury system, the Foodstuff Control, the Foreign Exchange Fund and the Trust Fund play important roles, both through their intra-governmental transactions and through their transactions with private sectors. Particularly noticeable is the role played by the Trust Fund Bureau, which serves as a financial institution for government agencies. Surplus and accumulated funds in the Postal Savings and other special accounts of the Government are deposited in the Trust Fund Bureau, which employs these funds for intra-governmental ways and means loans, and for government loans and investment programs.Another feature of Japan's Treasury system is that it deposits all the Treasury funds solely with the Bank of Japan.The activities of local authorities and local public enterprises are also largely financed by Treasury funds, and are intertwined with the Treasury system.The statistical systems for monetary and financial flow analysis developed by the Bank of Japan, therefore, place stress on the analysis of flows of Treasury funds, and are based on an institutional sectoring to reflect the flows of funds as they actually take place. One exception is the Monetary Survey compiled in accordance with the IMF formula, which adopts a kind of functional sectoring for international comparison purposes.In the last three years, Japan's public sector, which had long stood rather neutral in the financial patterns of the economy, has begun to show an increasing financial deficit. With the increasing financial deficit of the sector, the financial patterns of the nation as a whole are undergoing remarkable changes.

Notes: South Korea began its measurement of Gross National Product during the turbulent 1950's, a period of postwar rebuilding and of political and social changes. With only a small and largely inexperienced staff, and with little support from other statistical agencies whose data were essential to adequate GNP measurement, the Bank of Korea began this task in the early 1950's. Early estimates were extremely rough; over the years, the statistical staff was trained and other statistical agencies were upgraded. Measurements of output in the large agricultural sector and in manufacturing have gradually but consistently been strengthened as recent input-output data has been developed. Gaps still persist, particularly in the wholesale and retail sectors, but certain strengths are present: an outstanding job has been done in product pricing. The author describes the evolution of Korea's improving GNP program, presents its sources of data and its methodologies, and gives an assessment of problems of the past and prospects for the future.

Notes: This paper is concerned with the sensitivity of estimates of the aggregate capital stock of the United States to the statistician's choice of depreciation method. The usual depreciation charge can be shown to include allowances both for physical deterioration and for obsolescence. If one interprets the gross stock as the stock of surviving assets, then the various net stocks defined by depreciation accounting may be interpreted as a revaluation of these assets by means of an index of embodied technical change. Estimates of the United States capital stock were generated under eight sets of assumptions. These estimates are compared with respect to level, trend, and implications for other aggregate statistical indicators. The conclusion is reached that the assumptions which define a country's stock of tangible capital are of considerably greater importance than has often been supposed.

Notes: The contention in this paper is that the present method of treating interest and net rents as transfers rather than as payments for services provided creates problems in the measurement of production by industry and that the difficulties encountered in explaining the treatment of interest items in the different tables of the National Accounts are even more apparent when one views the National Accounts framework as an integrated reflection of economic reality.It is argued that the lending of money arises from the stretching out of the production and consumption process, and the interest charges constitute a charge for the administrative services and risk involved. This is somewhat analagous to the charges for hiring out real goods and services. A similar case is made for the treatment of rents with the exception of imputed net rent where it is contended that economic risk is incurred only when production is undertaken for sale and that there should be no entrepreneurial return where the production is for the use of the owner-producer.It is suggested that an alternative treatment of interest and rents as payments for services is more realistic. Its adoption in the National Accounts would eliminate the need for imputations now made to account for the production of financial intermediaries, as well as the unconvincing explanations put forward for the present treatment of interest on consumer and public debt. Finally, it would serve to integrate the production accounts with the financial flows and the related financial structure.

Notes: This paper is a part of a larger study of economic growth in Canada, following the methods developed by Edward Denison in his book The Sources of Economic Growth in the United States and the publication Why Growth Rates Differ. The new material in this paper relates to Canada and the Canadian/U.S. comparison, while the material on Northwest Europe is drawn from the Brookings study.The present paper sets out the results to date on the differences in real output per employed person between Canada and the United States for one year, 1960. At this stage in our research the results indicate that the level of real output per employed person in Canada was about 20 per cent lower than in the United States in that year. On the basis of historical output data, it would appear that this margin of difference in Canadian/U.S. product levels has persisted throughout the present century.The central part of this paper examines the significance of differences in factor inputs in Canada and the United States and their contribution to the difference in income. The level of inputs per employed person in Canada accounts for only about 2 percentage points of the income difference between Canada and the United States. These results indicate that the overwhelming part of the difference in output per employed person between the two countries reflects the differences in output in relation to total factor inputs, rather than the magnitude of other factor inputs used in combination with labour.This result is consistent with earlier studies by Denison and others which have indicated the crucial importance of output in relation to total factor inputs, both in output growth over time and intercountry comparisons of output level.The body of the paper can give only brief attention to the numerous conceptual and statistical questions that arise in such a wide-ranging study, and the authors do not pretend to have tackled, let alone resolved, all of the wide range of problems related to this study. Nor do they claim any high degree of precision for the results, especially in the light of the statistical limitations of the basic data.

Notes: Dudley Seers and his colleagues in working with various less developed economies have proposed a modified version of an input-output table for making projections and tests of consistency in planning. The table includes only the important inter-sectoral flows. By making a simplifying assumption with regard to the non-included inter-sectoral flows, an algebraic formulation of the modified input-output table is possible. The resulting matrix of input-output coefficients and final demands can be transformed into a Leontief input-output matrix which is block triangular and composed of two-blocks, one of which is diagonal. Given a set of final demands it is very easy to solve for the total output of each of the sectors. The amount of computation involved is directly related to the number of intersectoral flows included in the original modified input-output table.

Notes: Capital requirements may be expressed in various ways but when comparisons are to be made between situations of great variety it is best to express them per unit of annual product. The definition of product also raises difficulties. Here it is measured net of agricultural inputs but still gross of industrial inputs. The study approaches the problem by first considering the capital requirements of the simplest types of agriculture and then moving up the scale towards the more advanced. Simple forms of crop culture using hand tools may require only 0.1 to 0.2 of a year's product in the form of capital. These requirements increase when livestock are added, either for draught power or for their products. Increases also occur when tree and bush crops are introduced. In the less favourable climates, capital is also needed for the provision of shelter. When comparisons are possible between farms of different sizes, the greater capital requirements per unit of product for the smaller farms are clearly to be seen. In general the amount of capital per unit of product in agriculture is tending to fall, both through improvements in techniques and through an increase in the average size of holding.

Notes: The purpose of the paper is to describe current and constant price estimates of Japanese central and local government postwar domestic expenditures by economic type and function recently completed by Miss Yoshiko Kido, International Christian University, Tokyo, and myself. The rationale of the functional classification is to estimate those government expenditures which enhance the economy's productive capacity.Expenditures are divided into four broad functional categories: developmental, disaster repair and prevention, social welfare, and general government. These four categories are subdivided to two levels of disaggregation. We were able to break down government fixed investment, government enterprise inventory investment, current domestic transfers and subsidies into 42 functional components. For constant price series, each functional component by economic type was deflated by separate price indexes. We followed the Economic Planning Agency's procedure for the official national accounts of assuming no productivity change in the provision of government services.Our results are generally comparable to the official national accounts estimates. The major difference is that we attribute considerably more fixed investment to local governments, and correspondingly less to the central level.Government expenditures had the following characteristics. Growth was rapid; in real terms the public sector use of the economy's resources in 1963 was 2.2 times more than in 1952. The elasticity of government expenditures to GNP was unity in current prices, slightly less in real terms. The government postwar share in GNP has been smaller than in European nations and, unlike them, was not rising. This reflects the underlying growth strategy of emphasis upon private business fixed investment. Government consumption expenditures declined relative to GNP, and investment rose.Developmental expenditures comprised the largest share (40–45 per cent) of the government total. The elasticities to GNP of government expenditures by economic and functional categories are provided and discussed.A simple test was made of the cyclical relationship of government expenditures (both total and by category) to GNP. The results suggest that government expenditures, rather than contra-cyclical, were pro-cyclical in effect.

Notes: Economic planning can in principle be seen as consisting of two phases: first a description of all possible development paths, and second a choice of “the best one” from among these possibilities. In the present paper the measurement of real capital is discussed in relation to the needs of the first of these two phases. In section 2 of the paper it is argued that the most relevant measure of capital for this purpose is the gross value of the existing capital stock, i.e. the total value without accounting for depreciation. In section 3 of the paper different ways of estimating this gross capital stock are discussed. In sections 4, 5 and 6 there follows a discussion of how one can correct the capital measures for changes in efficiency with age, for “embodied technical progress” and for different durabilities. The latter correction leads to concepts which are equivalent to measuring “capital services” as a factor of production. The treatment of maintenance and repair will be important for the interpretations of some of these “corrections.” The final section of the paper suggests a model which requires data on vintages of capital.

Notes: The paper is concerned with non-monetized transactions which are dimensionally important in developing countries. The notion of degree of monetization attaches to all real flows. It is necessary to analyze non-monetized transactions in order to a have a better understanding of the producing and consuming activities of households which contribute a large part of national product in less developed countries. Among different non-monetized flows, particular attention is paid to the use of the output of own production for different purposes. A survey of Indian information on the degree of non-monetization shows that it is different for different flows: highest for consumption, intermediate for current inputs and lowest for investments. Cross section Indian data indicate that the degree of non-monetization is expected to fall with the improvement in the average household expenditure and urbanisation but it may rise if development occurs largely through agricultural improvement. Some of the Indian findings may apply to other developing countries as well. Normally, estimates of expenditure elasticities based on cross section data are obtained from consumption expenditure on a particular item (e) and the aggregate consumption expenditure (E) without going into the question of the degree of non-monetization of either element. Since traditional models of consumer behaviour apply only to the relation between money expenditure on a particular item (em) and the aggregate money expenditure (Em), it is suggested that the relation between e and E should be broken down into relations between (i) em and Em, (ii) ek and Ek where these are the corresponding kind elements and (iii)among E, Em and Ek. Some estimates of elasticity based on this scheme are presented indicating that the procedure is reasonable and suggesting that this type of analysis would probably furnish a suitable framework for answering relevant questions in the field.

Notes: 1. The problems presented have arisen in practice when carrying out international comparisons of national income and its elements between the CMEA countries. Some rough conclusions are drawn from the nearly completed comparison of consumption level between Poland and Austria.2. The basic methodological principles of the comparison were similar to the methods used by the group of economists directed by Milton Gilbert and Irving Kravis in their comparison relating to Western European countries. However, a number of new problems have emerged in the course of our work which required practical and theoretical solutions.Some differences in theoretical approach between the Gilbert-Kravis study and ours are discussed. Gilbert and Kravis based their comparison as far as possible on average prices of commodity groups or quantity data, and price indexes for representative goods were applied only as a practical necessity. On the contrary in our study we based our calculation mainly on representative goods and their price relation as this method, in our opinion, takes into account quality differences, which escape from the picture in the Gilbert-Kravis method.3. Some special theoretical and practical problems of comparisons between countries having market economies and those with planned economies are presented in terms of the example of the comparison of consumption levels between Austria and Poland.Three groups of questions are pointed out: (1) the problem of the definition and boundaries of the aggregates compared; (2) the problem of differences in pricing in the groups of products and services compared, resulting from the social policy of the government concerned; and (3) the problem of differences arising from general price policies in the countries compared.4. At the end of the paper it is suggested that it would be useful to work out a “statistical information system”, which would make possible detailed comparisons of the volume of consumption among several countries and groups of countries without the need of conducting direct comparisons between each pair of countries.

Notes: The principle of the mise-á-la-masse method is to earth one current electrode of a pair in a conducting mineral show (in a borehole, in an outcrop etc.) and measure the resulting distribution of electric potentials. The distribution will, to some extent, reflect the geometry of the ore mass of which the mineral show forms a part.In a mise-á-la-masse survey of a lead-zinc deposit in Central Sweden electric potentials were measured on the surface of the ground as well as in some 25 drillholes, in either case with earthings (successively) in three different parts of the irregular ore deposit. Besides this, measurements were made in drillholes with earthings in two further drillholes.Geologic correlation between the drillholes is difficult in the present case on account of the irregular geometry of the ore deposit. However, the mise-á-la-masse measurements clearly showed the dip and the pitch of the ore body, established connections between the different ore widths encountered in the various holes, and yielded information about the shape of the ore mass.In particular, the survey showed that the ore lenses must be crescent-shaped rather than tabular, and the dip was indicated to be westerly, instead of easterly as originally presumed.Three-dimensional models of equipotential surfaces were constructed from the observed drillhole and surface potentials (using transparent plastic sheets and thin copper wire) and these helped to elucidate the mass geometry further.The surface and underground potential data collected in the present case should be of nterest to geophysicists working on analytic continuation problems.

Notes: The author comments the techniques and methods used in continuous seismic profiling by the Monaco Oceanographic Museum during the last five years.Pinger probe give informations on the Mud layering and thickness. On the shelf or in abyssal plains, boomer and sparker are employed and allow to rise various geological conclusions. South coast of France, continental shelf in the Gulf of Lion, abyssal plain of the Ligurian Sea and Lake of Geneva are the areas concerned to with the results.

Notes: The author proposes the parameter Δ2/ΔT1 for possible application in aeromagnetic surveying making use of already available gradiometer systems equipped with sensors separated vertically.

Notes: Geophysical field equipment has undergone rapid changes in the past decade; from simple AGC amplifiers and galvo cameras to binary gain switching amplifiers and digital recorders, all in an attempt to keep pace with the new geophysical interpretive methods developed, and the growing acceptance of the terminology, methods, and philosophy of communication theory.The additional tools of the digital recorder and digital computer make it possible to utilize these new techniques in geophysical processing. Accomplishing these new techniques demands severe requirements on the digital field recording process in handling the decreasing energy return from the seismometer, and to fully realize the capabilities of digital techniques in reducing data. Simple automatic gain control may be used. However, in the more sophisticated interpretive methods, such as autoregression and deconvolution, it is necessary to reconstruct the actual energy levels in the computer. Recording the control signal used in master AGC or programmed gain control may prove satisfactory; however the accuracy of control versus gain is limited to I% for such analog instrumentation.To utilize the computer to its fullest extent, and to accurately perform these new techniques, requires an accuracy of I% or better. This accuracy is obtainable by using a step gain control where the gain is increased by fixed steps in which each step represents a gain in amplification by a constant factor. The accuracy in this case can be made dependent only on the tolerance of resistors used as attenuators or feedback elements. Preferably the constant factor of gain steps should be a number easily handled by the computer. By using 6 db steps it becomes a simple matter to shift binary numbers, such as multiplying or dividing by 10 in the decimal system.The requirements or parameters for such an amplifier system, and the elements of the amplifier necessary to achieve these requirements are presented.

Notes: Consideration is given to the use of a configuration of four electrodes set in a square array for resistivity measurements.It is found that, by passing current successively between different pairs of electrodes, an apparent resistivity can be determined which is both more sensitive to the position of the array centre and less dependent on orientation than the measures usually obtained with colinear arrays of electrodes. At the same time the observations made enable the degree of the departure of local conditions from conditions of lateral homogeneity to be assessed.Theoretical and practical examples of the use of this electrode system are given and the use of the system both as a tool in mapping and in depth investigations is considered.It is shown that provided electrode spacings are suitably arranged the results of a probe carried out using the square array can be interpreted by conventional methods.The system is shown to have particular advantages in the investigation of lateral resistivity variations and the reduced dependence on orientation makes possible the recasting of interpretation data in an orientationally invariant form with a consequent drastic reduction in the number of type curves required for a particular problem.

Notes: A large self-potential anomaly was outlined in 1963–64 at Tantahuatay near Hualgayoc in the Andes of northern Peru. Peak value recorded was–1842 millivolts–thus making it one of the strongest, or perhaps the strongest, SP anomaly ever measured. A lack of detailed geological data precludes the formulation of an adequate explanation for the Tantahuatay anomaly, but geological and mineralogical similarities with the well-documented Venencocha anomaly near Cerro de Pasco, Peru (Kruger and Lacy, 1949), suggest that the anomaly arises from sulfuric acid associated with the mineral alunite. The anomaly obviously cannot be explained by the half-cell mechanism of Sato and Mooney (1960), who place a limit of 700 millivolts on self-potential anomalies over sulfide bodies. Further study of the Tantahuatay anomaly would be of interest in understanding self-potential mechanisms in general.

Notes: Book review in this articleF. S. Grant and G. F. West, Interpretation Theory in Applied Geophysics, McGraw-Hill Comp., New York, 1965, 583 pp., price $ 17.50.Archaeo-Physica. Technische und naturwissenschaftliche Beiträge zur Feldarchäologie. Mit Beitragen von E. Le Borgne, I. Scollar, J. D. Mudie, J. Görier, H.-J. Appel, G. Strunk-Lichtenberg, Böhlau Verlag, Köln, Graz, 1965, 202 pp., 124 fig.H. Takeuchi, Theory of the Earth's Interior, Blaisdell Publishing Company, Waltham (Mass.), Toronto, London, 1966, 131 pp.G. D. Garland, The Earth's Shape and Gravity, The Commonwealth and International Library, Geophysics Division, Pergamon Press, Oxford, London, Edinburgh, New York, Paris, Frankfurt, 1965, 183 pp., price: 17s 6d.

Notes: The electromagnetic field radiated from a magnetic dipole lying on the ground is considered, in the extremely low frequency range (DC to 20 000 Hertz).Theoretical and experimental data are given on the characteristics of the surface wave (vanishing wave) generated at the air-ground interface, in the case of an homogeneous subsurface.The case of a subsurface with electrical resistivity varying with depth is considered.It is shown how the above-mentioned characteristics may be applied in the quantitative investigation of the electrical resistivity of the subsurface as a function of depth, in a method using the measurements on the ground of all the components of the radiated field: horizontal electrical components, vertical and horizontal magnetic components.

Notes: Book review in this articleHandbuch der Physik/Encyclopedia of Physics, Ed.: S. FlüggeThe Aurora. By S.-I. Akasofu, S. Chapman and A. B. Meinel.Time-Variations of Cosmic Rays. By S. E. Forbush.Magnetic Properties of Rocks and Minerals. By T. Nagata.

Notes: A formula is developed for the vertical magnetic field due to an alternating current passing through a long horizontal cable placed on a two-layer ground. The variations of the phase and amplitude ratio of the vertical field, along profiles perpendicular to the cable line are investigated. Within the limits encountered in practice, it is found that if the upper layer is conductive, as compared to the lower layer, the phase may vary appreciably whereas the amplitude ratio changes as in the case of vacuum.

Notes: Book review in this articleN. A. Ogil'vi, D. I. Fedorovich, Groundwater Seepage Rates. English translation by N. H. Choksy, Consultants Bureau, New York 1966, 33 pp., 12 fig.

Notes: Mapping of fault patterns is an important part of geophysical exploration. A computerized digital template analysis method is described which tests gravity maps for the effects of faults by comparing measured gravity data with calculated master curves. The interpreted gravity data are incorporated in a tectonic map using geological symbols and units, for ready use by the geologists.Tectonics can be investigated by gravity detailing if the smallest undulations on the Bouguer map are taken into consideration. Up to now, residual and derivative gravity maps have explained gravity effects by assuming spherical bodies which are almost unknown in geology. The method discussed here uses tectonic elements, such as fault-blocks and dikes as a basis of interpretation instead of the spherical bodies of the conventional interpretation methods.Gravity data can be easily and relatively cheaply obtained in the early phases of exploration by area wide spot coverage following lines of easy access such as roads, etc. Seismic studies, by contrast, have the disadvantage of being executed along predetermined profile lines. Only after sufficient detailing do seismic profiles permit-if at all -an areal mapping of faults. Thus a tectonic map is obtained only at the end of a geophysical survey instead of being available prior to the planning of costly seismic profiles. The use of gravimetric data and their interpretation by the suggested method provides tectonic detail maps in the early phases of geophysical studies.In addition, this article discusses a general geophysical interpretation method, using the investigation of faults by gravity as an example. Applications of this method for different gravity and magnetics problems as well as for combined interpretations are outlined. Detailed case histories will be published in later articles.

Notes: To be able to make use of all existing probe interpretation techniques, it is desirable that field resistivity observations be conducted in such a way that it is possible to construct an equivalent curve for the surface variation of potential about a point source. Further, the usual parallel layer interpretation will only be justified in as much as the observed potential curve is compatible with such a subsurface geometry. Thus, whilst a potential curve may be constructed from suitable finite potential differences obtained using a Wenner configuration of electrodes, it can be shown, using the tri-potential technique of measurements, that these potential differences may arise partly from lateral resistivity variations.In this paper, a ladder network technique is employed to display these lateral effects and an adjustment method proposed to reduce them. The adjusted potential differences obtained form a consecutive series suitable for summation to give the potential function. These values are subject to further minimum adjustments required for them to comply with slope and curvature conditions for a layered medium. After forming the potential curve by summation, a final numerical smoothing process is carried out. From this smoothed potential curve, corresponding Wenner and Schlumberger curves can readily be derived for interpretation.The method proposed thus attempts to extract from the observed data the maximum part which can be reconciled with a purely depth variation of resistivity. A method is also proposed for compounding the ‘errors of closure’ of the network to provide a Lateral Inhomogeneity Index which gives a measure of the departure of the observed data from the basic interpretation requirements.The method involves no great labour, but can readily be programmed for a computer if desired. Examples are given of the application of the method to field observations. In the interest of objectivity, the final smoothing has been confined to a single stage but in certain very extreme examples a further stage may prove desirable.

Notes: Tall fescue and Italian ryegrass mixtures react differently to management in the year of sowing. The decrease in yield of tall fescue and the increase in growth of red clover, caused by the use of a cover crop, was still evident in the following year. In both the spring grazing and total yield of the first harvest year, the Italian ryegrass/ white clover mixture was better than the tall fescue/white clover mixture when a cover crop was used. The reverse trend was recorded when the grass plus clover was sown without the cereal. In general, the addition of red clover to the tall fescue/ white clover, or Italian ryegrass/white clover mixture, increased the yield, but the magnitude of the increase was modified by management during establishment. The increase in total herbage yield and the reduction of white clover growth due to nitrogen application were both related to management in the year of sowing. Consideration should thus be given to method of establishment in the assessment of herbage seeds mixtures.

Notes: Germination results are presented for S48 timothy seed dried at air temperatures of from 75 to 135° after direct harvesting at 3 moisture levels, followed by cold-air conditioning for periods of from 1 to 3 days. The effect of conditioning was to increase the resistance of the seed to high drying-air temperatures, without producing any real improvement at low temperatures.The results for non-conditioned seed, published earlier, are converted to simple chart form giving the limits of initial moisture content and drying-air temperature for 90% germination. These charts can be used during the final drying of conditioned seed, provided that the original moisture content at harvest is within the “safe” range.

Notes: The development and testing of a new silage additive consisting of sodium nitrite and hexamine (hexamethylenetetramine) are described. The investigation was conducted over 8 years and consisted of 15 separate experiments with small steel cylinders and 13 with plastic sacks. The former were carried out with freshly cut blue lucerne and a storage temperature of 25°. When a mixture of sodium nitrite and hexamine was applied at the rate of 0.4% of fresh herbage weight, the quality of the resulting silage was superior to that made with commercial additives marketed in Sweden and the losses were considerably reduced.

Notes: Absorption by the tap-root and by different nodal roots of white clover S100 and translocation of 32P were investigated to find to what extent nodal roots can compensate for the absence of a tap-root. 32P absorbed from the tap-root was distributed evenly within the whole plant. When translocation from the 2nd and 7th nodal roots was studied, similar distribution was obtained only from the nodal root closer to the centre of the plant. The backward movement of 32P absorbed from nodal roots at the base of the plant increased as the root size increased. Removal of tap-roots resulted in temporary depression of translocation; its effect disappeared within 3 weeks.It is concluded that the ability of nodal roots to compensate for loss of the tap-root depends on their position and size.

Notes: In a recent paper by Anslow it was concluded that there was no significant relationship between Leaf-area Index, light interception and growth rate in a perennial ryegrass sward. The author further concluded that higher growth rates would not be obtained by attention to this relationship. The data presented by Anslow are re-examined in this paper and the results of the analysis showed that: (a) recovery growth of the swards followed a sigmoid pattern of growth, (b) at low values of LAI growth rate was low and maximum growth rate was attained when most of the visible light was intercepted by leaves, and (c) the relationship between yield and LAI was linear, indicating that leaf-area increase after defoliation also followed a sigmoid pattern. These results are discussed in relation to Anslow's interpretation.

Notes: An account is given of a method for measuring short-grazed pastures. The procedure has particular value in studying the relationship of animal performance to pasture availability. A number of other pasture characteristics may be measured concurrently. These include species composition, tiller number and mean weight, stem-base material, roots, rhizomes and plant litter. The influence of core size and shape is studied and variance components reported.

Notes: Data from two field experiments, and from a root observation trench, suggest an annual cycle of root growth by grass swards. The production of adventitious roots from the tillers of four grass species, and from perennial ryegrass in a grass/clover sward varied greatly during the year. They were produced in increasing numbers through late winter to early spring; this rate fell sharply in April or May, and was low in summer. The differences in this annual cycle between grass species were small. Examination of roots of perennial ryegrass in a glass-sided trench showed that root elongation was most rapid in summer and was largely confined to the lower soil horizons. Rather more roots were produced in early autumn than during summer, but the rate of elongation was slow from October until spring.Individual roots of grasses appeared to survive for limited periods. Their longevity depended upon the time of year in which they were first formed; those produced in autumn or winter lived longer than those produced in spring or summer.

Notes: The root growth of 3 temperate–region grasses (perennial ryegrass, Italian ryegrass, and cocksfoot) and of 3 tropical grasses (Dallis grass, Bahia grass, and Rhodes grass) was investigated in large glass–sided root–boxes. The trend of root numbers and the rate of elongation of roots were ascertained to discover how the Japanese climate affected the root growth of these species.All the temperate–region grasses produced new roots vigorously during spring, but root growth ceased in August, whereas the roots of tropical grasses grew rapidly in this month. The alternate use of these two types of grasses during the growing season may therefore be worthwhile in Japan

Notes: In one geophysical survey over a salt dome in the Gulf of Mexico, data taken from a single magnetic and seismic shipborne traverse demonstrated significantly the importance of magnetic measurements. The reduced magnetic and seismic profiles were integrated with gravity measurements from an earlier survey demonstrating the value of obtaining simultaneous geophysical data. The traverse was approximately 25 miles in length, 135 miles southeast of Galveston, Texas. The observed gradient averaged approximately 2 gammas per 1000 feet and crosses the southwest corner of a large shallow salt dome called the Way Dome. The recorded magnetic profile over the salt dome shows a negative magnetic anomaly extending for approximately 12 miles with a maximum amplitude of approximately 20 gammas. This negatively anomaly is due to the negative susceptibility contrast between the salt and cap rock that truncates the surrounding parent sediments. The shape of the negative anomaly correlates well with the continous seismic record and clearly delineates the topographical and structural features of the salt dome. The recorded analog magnetic profile was replotted, with both the regional gradient and time variations removed, which makes the observed anomaly much sharper than is noticeable on the original recorded strip chart record. A theoretical magnetic anomaly was calculated using a computer program for comparison to the observed anomaly. The results showed good correlation.

Notes: Amplitudes of the vertical ground motion are larger in the critical angle region than in the near vertical region, especially amplitudes coming from deeper boundaries or interfaces with small velocity contrast. Four basic boundary models are used to derive specific travel time patterns for first order and higher order interfaces. The transition from reflected waves to diving waves in the supercritical angle region is shown for the different models.Examples from wide angle surveys in Bavaria and in the Buchara region in the USSR have many characteristic similarities. Travel time patterns and asymptotic velocities are almost identical. The most important boundaries of the continental crust - the Mohorovč Ić discontinuity and the top of the crystalline basement - correspond to two boundary models mentioned before. Amplitude and frequency investigations have been used to derive items of the M-discontinuity. It is certainly a gradient zone and seems to have a lenticular structure with material of stepwise different velocities.