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  • wheat  (807)
  • Springer  (807)
  • American Chemical Society
  • American Institute of Physics (AIP)
  • Blackwell Publishing Ltd
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  • 101
    Electronic Resource
    Electronic Resource
    Isolation and analysis of a cDNA clone encoding the small subunit of ADP-glucose pyrophosphorylase from wheat (1993)
    Springer
    Plant molecular biology 23 (1993), S. 23-33 
    Details
    ISSN: 1573-5028
    Keywords: wheat ; Triticum aestivum ; cDNA clone ; ADP-glucose pyrophosphorylase ; small-subunit ; starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A full-length cDNA clone from hexaploid bread wheat, encoding the small subunit of ADP-glucose pyrophosphorylase, has been isolated from an endosperm cDNA library. The cDNA insert has an open reading frame which encodes a protein of 473 amino acids (52.1 kDa). The presence of a chloroplast/amyloplast transit peptide of 22 amino acids is proposed. The deduced amino acid sequence exhibits a high degree of homology with the small subunit ADP-glucose pyrophosphorylase proteins from rice (with 90% of identical amino acids) and potato (with 86% of identical amino acids) and contains conserved sequence elements which are thought to represent the substrate binding and allosteric activator sites. The genes are organised as single-copy loci on chromosomes 7A, 7B and 7D in the wheat genome and are highly expressed during grain development. Homologous transcripts are expressed in leaves and roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021416
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  • 102
    Electronic Resource
    Electronic Resource
    cDNA encoding a wheat (Triticum aestivum cv. Chinese Spring) glycine-rich RNA-binding protein (1996)
    Springer
    Plant molecular biology 30 (1996), S. 1301-1306 
    Details
    ISSN: 1573-5028
    Keywords: abscisic acid ; glycine-rich ; ribonucleoprotein ; RNA-binding protein ; RNP motif ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A wheat cDNA encoding a glycine-rich RNA-binding protein, whGRP-1, was isolated. WhGRP-1 contains two conserved domains, the RNA-binding motif (RNP motif) combined with a series of glycine-rich imperfect repeats, characteristic of a conserved family of plant RNA-binding proteins. Northern analysis revealed that whGRP-1 mRNA accumulates to high levels in roots and to lower levels in leaves of wheat seedlings. whGRP-1 mRNA accumulation is not enhanced by exogenous abscisic acid in seedlings and accumulates to very high levels during wheat embryo development, showing a pattern different from that of the ABA-inducible wheat Em gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019560
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  • 103
    Electronic Resource
    Electronic Resource
    Differential display-mediated rapid identification of different members of a multigene family, HSP16.9 in wheat (1996)
    Springer
    Plant molecular biology 31 (1996), S. 575-584 
    Details
    ISSN: 1573-5028
    Keywords: differential display ; heat shock proteins ; non-coding regions ; PCR ; reverse transcription ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolation of cDNAs encoding individual members of a gene family is essential for assessing their role in a biological phenomenon. However, this process is often laborious and slow due to highly conserved protein-coding region that interferes with the isolation of the individual members. Identification of gene-specific probes from 3′ non-coding regions of different members can assist in the fast retrieval and characterization of individual members of a multigene family. We used the recent technique of differential display for the same purpose. As an example of a multigene family in plants, we selected a heat shock protein gene family, HSP16.9 from wheat, with estimated 12 members. We modified the original differential display technique for selective amplification of the 3′ non-coding regions of different wheat HSP16.9 genes by replacing the random 10-mer in the original method with a conserved HSP16.9 gene family-specific primer. Sixteen cDNA fragments from these experiments were sequenced and they represent 8 different members of a 12 member gene family. Our succes can be attributed to shorter 3′ non-coding regions that are typical of higher-plant genes and use of highly conserved gene family-specific primer in these experiments. This modified differential display technique can be of general application to other plant systems where cloning of the different members of a gene family is desired.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00042230
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  • 104
    Electronic Resource
    Electronic Resource
    A pollen allergen-encoding gene is expressed in wheat ovaries (1996)
    Springer
    Plant molecular biology 32 (1996), S. 435-445 
    Details
    ISSN: 1573-5028
    Keywords: male sterility ; ovary ; parthenogenetic lines ; pollen allergen ; subtractive hybridization ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To isolate genes specifically expressed at the initiation of plant embryo development we have applied a sensitive subtractive hybridization technique for three isogenic wheat lines of the so-called ‘Salmon system’ with either zygotic or autonomous embryo development. Here we present a gene sequence showing a high homology to grass pollen allergens of type II/III thought to be expressed in pollen tissue only. Surprisingly, the pollen allergen-like sequence, designated Tri a III, is also expressed in gynoecia of the sexual, male fertile wheat line ‘(aestivum)-Salmon’, whereas the two parthenogenetic and male sterile wheat lines ‘(caudata)-Salmon’ and ‘(kotschyi)-Salmon’ completely lack any Tri a III transcript. Our data suggest a positive correlation between the expression of this clone and the manifestation of male fertility. Northern and in situ hybridization analysis revealed that, in addition to its presence in pollen, Tri a III is expressed in the parenchymatous tissue of ‘(aestivum)-Salmon’ ovaries exclusively at the day of anthesis. This precise temporal and spatial expression pattern suggests a more general function of the pollen allergen-like sequence Tri a III not limited to the exhibition of allergens in pollen grains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019095
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  • 105
    Electronic Resource
    Electronic Resource
    Expression of a wheat ADP-glucose pyrophosphorylase gene during development of normal and water-stress-affected anthers (1997)
    Springer
    Plant molecular biology 34 (1997), S. 445-453 
    Details
    ISSN: 1573-5028
    Keywords: ADP-glucose pyrophosphorylase ; anther ; pollen ; male sterility ; water stress ; wheat ; starch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In wheat (Triticum aestivum L.), water deficit during meiosis in the microspore mother cells (MMCs) induces pollen abortion, resulting in the failure of fertilization and a reduction in grain set. In stressed plants, meiosis in MMCs proceeds normally but subsequent pollen development is arrested. Unlike normal pollen grains, which accumulate starch during the late maturation phase, stress-affected anthers contain pollen grains with little or no starch. Stress also alters the normal distribution of starch in the anther wall and connective tissue. To determine how starch biosynthesis is regulated within the developing anthers of stressed plants, we studied the expression of ADP-glucose pyrophosphorylase (AGP), which catalyzes the rate limiting step of starch biosynthesis. Two partial-length cDNAs corresponding to the large subunit of AGP were amplified by RT-PCR from anther RNA, and used as probes to monitor AGP expression in developing anthers of normal and water-stressed plants. These clones, WAL1 and WAL2, had identical deduced amino acid sequences and shared 96% sequence identity at the nucleic acid level. In normal anthers, AGP expression was biphasic, indicating that AGP expression is required for starch biosynthesis both during meiosis and later during pollen maturation. AGP expression in stressed anthers was not affected during the first phase of starch accumulation, but was strongly inhibited during the second phase. We conclude from these results that the reduced starch deposition later in the development of stressed pollen could be the result of a lower expression of AGP. However, this inhibition of AGP expression is unlikely to be the primary cause of male sterility because anatomical symptoms of pollen abortion are observed prior to the time when AGP expression is inhibited.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005882118506
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  • 106
    Electronic Resource
    Electronic Resource
    The amino acid sequence and reactive site of a single-headed trypsin inhibitor from wheat endosperm (1994)
    Springer
    The protein journal 13 (1994), S. 187-194 
    Details
    ISSN: 1573-4943
    Keywords: Trypsin inhibitor ; wheat ; primary structure ; reactive site ; Bowman-Birk
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The sequence of a trypsin inhibitor, isolated from wheat endosperm, is reported. The primary structure was obtained by automatic sequence analysis of the S-alkylated protein and of purified peptides derived from chemical cleavage by cyanogen bromide and digestion withStaphylococcus aureus V8 protease. This protein, named wheat trypsin inhibitor (WTI), which is comprised of a total of 71 amino acid residues, has 12 cysteines, all involved in disulfide bridges. The primary site of interaction (reactive site) with bovine trypsin has been identified as the dipeptide arginyl-methionyl at positions 19 and 20. WTI has a high degree of sequence identity with a number of serine proteinase inhibitors isolated from both cereal and leguminous plants. On the basis of the findings presented, this protein has been classified as a single-headed trypsin inhibitor of Bowman-Birk type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01891977
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  • 107
    Electronic Resource
    Electronic Resource
    Multiple sequence rearrangements accompanying the duplication of a tRNAPro gene in wheat mitochondrial DNA (1988)
    Springer
    Plant molecular biology 11 (1988), S. 833-843 
    Details
    ISSN: 1573-5028
    Keywords: mitochondrial DNA ; transfer RNA genes ; rearrangement ; recombination ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the course of isolating tRNA genes from wheat mtDNA, we have found the same tRNAPro gene in two different Hind III restriction fragments, H-P1 (0.7 kbp) and H-P2 (1.7 kbp). Sequences immediately flanking these duplicate genes are closely related, although not identical; sequence comparisons suggest that multiple rearrangements have occurred in the vicinity of the H-P2 tRNAPro gene, relative to the H-P1 version. The chimeric nature of H-P2 is emphasized by the presence of sequences that are also found upstream of the wheat mitochondrial 26S rRNA gene, as well as sequences derived from chloroplast DNA. Comparison of H-P2 with H-P1 plus upstream sequences provides some insight into possible molecular events that might have generated H-P2. In particular, such comparisons suggest a model in which the homologous sequences in H-P2 are seen to be derived from H-P1 plus upstream sequences as a result of an intragenomic, site-specific rearrangement event, followed by amplification of the product, its fixation in the mitochondrial genome, and subsequent sequence divergence (single base changes as well as insertions/deletions of up to 50 nucleotides). The results reported here implicate particular primary sequence motifs in certain of the rearrangements that characterize H-P2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019523
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  • 108
    Electronic Resource
    Electronic Resource
    Cloning of cDNA and chromosomal location of genes encoding the three types of subunits of the wheat tetrameric inhibitor of insect α-amylase (1990)
    Springer
    Plant molecular biology 14 (1990), S. 845-853 
    Details
    ISSN: 1573-5028
    Keywords: α-amylase tetrameric inhibitor ; cDNA cloning ; genetic mapping ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have characterized three cDNA clones corresponding to proteins CM1, CM3 and CM16, which represent the three types of subunits of the wheat tetrameric inhibitor of insect α-amylases. The deduced amino acid sequences of the mature polypeptides are homologous to those of the dimeric and monomeric α-amylase inhibitors and of the trypsin inhibitors. The mature polypeptides are preceded by typical signal peptides. Southern blot analysis of appropriate aneuploids, using the cloned cDNAs as probes, has revealed the location of genes for subunits of the CM3 and of the CM16 type within a few kb of each other in chromosomes 4A, 4B and 4D, and those for the CM1 type of subunit in chromosomes 7A, 7B and 7D. Known subunits of the tetrameric inhibitor corresponding to genes from the B and D genomes have been previously characterized. No proteins of this class have been found to be encoded by the A genome in hexaploid wheat (genomes AA, BB, DD) or in diploid wheats (AA) and no anti α-amylase activity has been detected in the latter, so that the A-genome genes must be either silent (pseudogenes) or expressed at a much lower level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016517
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  • 109
    Electronic Resource
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    Cloning and sequencing of cDNAs encoding a pathogen-induced putative peroxidase of wheat (Triticum aestivum L.) (1991)
    Springer
    Plant molecular biology 16 (1991), S. 329-331 
    Details
    ISSN: 1573-5028
    Keywords: pathogen-induced ; peroxidase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report here the complete amino acid sequence of a pathogen-induced putative peroxidase from wheat (Triticum aestivum L.) as deduced from cDNA clones representing mRNA from leaves infected with the powdery mildew fungus Erysiphe graminis. The protein consists of 312 amino acids, of which the first 22 form a putative signal sequence, and has a calculated pI of 5.7. Sequence comparison revealed that the putative wheat peroxidase is most similar to the turnip (Brassica rapa) peroxidase, with which it shares 57% identical and 13% conserved amino acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020563
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  • 110
    Electronic Resource
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    Sequence and tissue-specific expression of a putative peroxidase gene from wheat (Triticum aestivum L.) (1991)
    Springer
    Plant molecular biology 16 (1991), S. 171-174 
    Details
    ISSN: 1573-5028
    Keywords: peroxidase gene ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used a cDNA clone encoding a pathogen-induced putative wheat peroxidase to screen a genomic libary of wheat (Triticum aestivum L. cv. Cheyenne) and isolated one positive clone, lambda POX1. Sequence analysis revealed that this clone contains a gene encoding a putative peroxidase with a calculated pI of 8.1 which exhibits 58% and 83% sequence identity to the amino acid sequence of the turnip (Brassica rapa) peroxidase and a pathogen-induced putative wheat peroxidase, respectively. The two introns in the wheat gene are at the same positions as introns in the peroxidase genes of tomato and horseradish. Results of S1-mapping experiments suggest that this gene is neither pathogen-nor wound-induced in leaves but is constitutively expressed in roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017928
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  • 111
    Electronic Resource
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    Sequence analysis of a cDNA encoding a Group 3 LEA mRNA inducible by ABA or dehydration stress in wheat (1991)
    Springer
    Plant molecular biology 16 (1991), S. 1073-1076 
    Details
    ISSN: 1573-5028
    Keywords: abscisic acid ; dehydration ; LEA ; water stress ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone (pMA2005) of a Group 3 LEA (late embryogenesis abundant) protein has been sequenced from wheat. The wheat cDNA clone codes for a protein with ten tandem repeats of an 11 amino acid sequence and has homology to other Group 3 LEAs reported in barley, carrot, cotton and rape (L. Dure et al., Plant Mol Biol 12: 475–486, 1989). The deduced amino acid sequence indicates that the wheat protein has a molecular weight of 23 000 and is a basic, hydrophilic protein. Northern analysis with the cDNA clone shows that dehydration of wheat shoot tissue results in increased transcript levels that correlate with increases in endogenous ABA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016078
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  • 112
    Electronic Resource
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    A chimeric gene (orf256) is expressed as protein only in cytoplasmic male-sterile lines of wheat (1994)
    Springer
    Plant molecular biology 26 (1994), S. 535-539 
    Details
    ISSN: 1573-5028
    Keywords: cytoplasmic male sterility ; coxI ; mitochondria ; membrane protein ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondria derived from Triticum timopheevi have a chimeric gene, orf256, immediately upstream from coxI. Antibodies to a peptide corresponding to a part of the encoded amino acid sequence of orf256 detect a 7 kDa protein on western blots of mitochondrial proteins from cytoplasmic male-sterile (cms) wheat (T. aestivum nucleus, T. timopheevi mitochondria) but not in mitochondrial proteins from T. aestivum, T. timopheevi, or cms plants restored to fertility by introduction of nuclear genes for fertility restoration. The 7 kDa protein appears to serve as a marker for cms wheat. Its occurrence as an integral protein of the inner membrane may indicate a cms effect through an influence on mitochondrial membrane function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039566
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  • 113
    Electronic Resource
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    Sharp divergence between wheat and barley at loci encoding novel members of the trypsin/α-amylase inhibitors family (1994)
    Springer
    Plant molecular biology 26 (1994), S. 1231-1236 
    Details
    ISSN: 1573-5028
    Keywords: chromosome mapping ; inhibitors of trypsin/α-amylases ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Amino acid sequences for three members (CMx1, CMx2, and CMx3) of a new subfamily of trypsin/α-amylase inhibitors in wheat have been deduced from the nucleotide sequences of the corresponding cDNAs. A cDNA clone encoding CMx1 was selected from a wheat developing endosperm library using a probe that encoded barley trypsin inhibitor BTI-CMe at low stringency. Sequences corresponding to CMx2 and CMx3 were obtained from cDNA amplified by the polymerase chain reaction. The three CMx sequences contain a premature stop codon after 363 nt, as well as a second stop codon at the same position as in BTI-CMe (nt 439–441). Southern analysis of DNAs from diploid, tetraploid, and hexaploid wheats, as well as from aneuploid lines, indicate that there is a single CMx locus in each of the three genomes of hexaploid wheat, respectively associated with chromosomal arms 4AS, 4BS, and 4DL. These genes are expressed early during endosperm development and not expressed at detectable levels in other tissues. Evolutionary implications are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040705
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  • 114
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    Developmental, circadian and light regulation of wheat ferredoxin gene expression (1995)
    Springer
    Plant molecular biology 27 (1995), S. 293-306 
    Details
    ISSN: 1573-5028
    Keywords: ferredoxin ; PetF gene ; circadian rhythm ; light regulation ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genomic clone encoding the precursor of wheat leaf ferredoxin has been isolated and characterised. The uninterrupted PetF gene encodes a polypeptide of 143 amino acid residues, consisting of an N-terminal presequence of 46 amino acid residues and a mature polypeptide of 97 amino acid residues. Southern blot analysis suggests that six copies of the PetF gene are present in the wheat haploid genome. Northern blot analysis has shown that the genes are both developmentally and light regulated in wheat seedlings and provides evidence that a circadian rhythm regulates the steady-state levels of ferredoxin transcripts. The intact wheat gene and several chimeric constructs, containing portions of the 5′-upstream region fused to the β-glucuronidase reporter gene, have been introduced into tobacco plants, but levels of β-glucuronidase activity above background were not detected, suggesting that the 5′-upstream region is unable to function as a promoter in tobacco plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020184
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  • 115
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    Multiple cDNAs of wheat voltage-dependent anion channels (VDAC): Isolation, differential expression, mapping and evolution (1995)
    Springer
    Plant molecular biology 29 (1995), S. 109-124 
    Details
    ISSN: 1573-5028
    Keywords: cDNAs ; expression ; mapping porin ; VDAC ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mitochondrial outer membrane of eukaryotic cells contains voltage-dependent anion channels (VDAC) also termed porins. Three cDNAs from wheat (Triticum aestivum) were isolated and sequenced (Tavdac 1–3). They share 65% similarity of their amino acid sequences, and therefore they probably represent isoforms. The deduced amino acid sequence of one of the cDNAs was found to be identical to the purified VDAC protein from wheat mitochondria [8]. Secondary structure analysis of the deduced amino acid sequences of the three vdac cDNAs revealed a characteristic α helix at their N-terminal and β-barrel cylinders characteristic of VDAC channels. The Tavdac cDNAs are differentially expressed in meristematic tissues. The transcript levels of Tavdac 1 in all wheat tissues is at least 2.5-fold higher than Tavdac 2 and Tavdac 3. Tavdac 2 has a low level of expression in all floral tissues whereas Tavdac 3 is highly expressed in anthers. This is the first report on differential expression of vdac genes in plants. The Tavdac genes have been mapped on the wheat genome. Tavdac 1 is located on the long arm of chromosome 5, Tavdac 2 on the long arm of chromosome 1 and Tavdac 3 on the long arm of chromosome 3. A phylogenetic reconstruction indicates that vdac genes underwent numerous duplication events throughout their evolution. All duplications occurred after the separation of plants from animals and fungi, and no orthologous genes are shared among phyla. Within plants, some of the vdac gene duplications probably occurred before the monocotydelon-dicotydelon split.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019123
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  • 116
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    A novel plant peptidyl-prolyl-cis-trans-isomerase (PPIase): cDNA cloning, structural analysis, enzymatic activity and expression (1996)
    Springer
    Plant molecular biology 32 (1996), S. 493-504 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; expression ; FKBP ; PPIase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel cDNA encoding for a peptidyl-prolyl-cis-trans-isomerase (PPIase) belonging to the FK506-binding protein (FKBP) family was isolated from wheat. It contains an open reading frame of 559 amino acids and it represents the first plant FKBP-PPIase to be cloned. It possesses a unique sequence which is composed of three FKPB-like domains, in addition to a putative tetratricopeptide repeat (TPR) motif and a calmodulin-binding site. The recombinant FKBP-PPIase expressed in and purified from Escherichia coli exhibits PPIase activity that is efficiently inhibited by the immunosuppressive drugs FK506 and rapamycin. Northern blot analysis showed that wheat FKBP was found mainly in young tissues. Polyclonal antibodies revealed the presence of cross-reacting proteins in embryos, roots and shoots. The unique structural features, the enzymatic activity and the presence of putative isoforms in wheat tissues indicate the possibility of the involvement of wheat PPIase in essential biological functions, similar to other members of the FKBP gene family.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019101
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  • 117
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    Characterization of dynamics of the psbD light-induced transcription in mature wheat chloroplasts (1997)
    Springer
    Plant molecular biology 33 (1997), S. 267-278 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; in vitro transcription ; light ; psbA ; psbD/C operon ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dynamical aspects of three chloroplast promoters responding to change in light condition were examined in mature chloroplasts of wheat (Triticum aestivum) by in vitro transcription. The wheat psbD/C operon has four distinct promoters, two of which named as D/C-3 and D/C-4 promoters dominantly function in mature chloroplasts to produce the mRNAs encoding D2/CP43 and CP43 alone, respectively. Activity of the D/C-3 promoter in mature chloroplasts was reduced to less than 30% by 24 h dark adaptation and recovered by re-illumination to the original level within 30 to 60 min. The activation of the D/C-3 promoter which requires de novo cytoplasmic protein synthesis was induced by low fluence of light (e.g. 16 µE m-2 s-1), but the extent of activation increased with increasing light fluence. The accumulation of mRNAs from the D/C-3 promoter saturated at 2- to 3-fold higher level within 2 h when the dark-adapted seedlings were transferred to the lig at 72 µE m-2 s-1, concomitant with the increase in rate of D2 synthesis, suggesting that synthesis of D2 in mature chloroplasts is controlled via the D/C-3 promoter activity in a light-dependent way. Activity of the D/C-4 promoter slightly increased in the dark and decreased in the light. Effect of light on the psbA promoter activity was not observed at all in mature chloroplasts. In vitro transcriptional analysis of the D/C-3 promoter with 5′ deletion mutations revealed that at least two cis elements which are located within the sequences of -78 to -47 and -46 to -29 of the transcription initiation site, respectively, act as enhancing elements in the D/C-3 promoter. The light-switching element of the transcription, however, was suggested to be located in the core promoter sequence downstream of the -35 element.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005799001271
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  • 118
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    Genes for tRNAAsp, tRNAPro, tRNATyr and two tRNAsSer in wheat mitochondrial DNA (1988)
    Springer
    Plant molecular biology 10 (1988), S. 251-262 
    Details
    ISSN: 1573-5028
    Keywords: mitochondria ; mitochondrial DNA ; transfer RNA ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have begun a systematic search for potential tRNA genes in wheat mtDNA, and present here the sequences of regions of the wheat mitochondrial genome that encode genes for tRNAAsp (anticodon GUC), tRNAPro (UGG), tRNATyr (GUA), and two tRNAsSer (UGA and GCU). These genes are all solitary, not immediately adjacent to other tRNA or known protein coding genes. Each of the encoded tRNAs can assume a secondary structure that conforms to the standard cloverleaf model, and that displays none of the structural aberrations peculiar to some of the corresponding mitochondrial tRNAs from other eukaryotes. The wheat mitochondrial tRNA sequences are, on average, substantially more similar to their eubacterial and chloroplast counterparts than to their homologues in fungal and animal mitochondria. However, an analysis of regions ∼ 150 nucleotides upstream and ∼ 100 nucleotides downstream of the tRNA coding regions has revealed no obvious conserved sequences that resemble the promoter and terminator motifs that regulate the expression of eubacterial and some chloroplast tRNA genes. When restriction digests of wheat mtDNA are probed with 32P-labelled wheat mitochondrial tRNAs, 〈20 hybridizing bands are detected, whether enzymes with 4 bp or 6 bp recognition sites are used. This suggests that the wheat mitochondrial genome, despite its large size, may carry a relatively small number of tRNA genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027402
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  • 119
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    Comparison of the wheat mitochondrial atp9 gene sequence with mitochondrial and chloroplast homologues from other plants (1989)
    Springer
    Plant molecular biology 13 (1989), S. 395-397 
    Details
    ISSN: 1573-5028
    Keywords: F1F0 ATPase ; chloroplast coupling factor ; mitochondrial gene ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015551
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  • 120
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    Characterization of the wheat mitochondrial orf25 gene (1990)
    Springer
    Plant molecular biology 15 (1990), S. 793-795 
    Details
    ISSN: 1573-5028
    Keywords: mitochondrial gene ; ORF25 ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The wheat mitochondrial orf25 nucleotide sequence of 576 pb has been determined. Its derived protein sequence shares 88% and 75% amino acid identity with those of maize and tobacco mitochondria, respectively. The wheat and tobacco orf25 sequences lack four inserts, of 6 bp to 36 bp, that are present in the maize homologue. The wheat orf25 gene is actively transcribed and is preceded by a regulatory sequence block very similar to those located upstream of the wheat coxII and atp6 genes. Our observations support the view that orf25 sequences encode a functional polypeptide in plant mitochondria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016131
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    A novel proline-rich protein from wheat (1991)
    Springer
    Plant molecular biology 16 (1991), S. 663-670 
    Details
    ISSN: 1573-5028
    Keywords: gene expression ; proline-rich protein ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA (WPRP1) encoding a wheat proline-rich protein has been isolated and sequenced. The amino acid composition shows 45% proline, with high levels of methionine, lysine and glutamic acid. The derived 378 residue amino acid sequence has a highly repetitive structure which is unlike those of other proline-rich proteins. The WPRP1 cDNA clone was used to determine the copy number and chromosomal location of the WPRP1 gene by restriction fragment length polymorphism analysis of wheat inbred lines. Although WPRP1 is encoded by a single-copy gene it is also a representative of a larger family of related sequences. RNA gel blot analysis showed that expression of WPRP1 is highest in rapidly growing tissue which together with its amino acid composition suggests a structural role for the encoded protein.
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    URL: http://dx.doi.org/10.1007/BF00023430
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    A wheat glutathione-S-transferase gene with transposon-like sequences in the promoter region (1991)
    Springer
    Plant molecular biology 16 (1991), S. 1089-1091 
    Details
    ISSN: 1573-5028
    Keywords: wheat ; glutathione-S-transferase ; transposon-like sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016083
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    Nucleotide sequence of a wheat (Triticum aestivum L.) cDNA clone encoding the waxy protein (1991)
    Springer
    Plant molecular biology 16 (1991), S. 1099-1101 
    Details
    ISSN: 1573-5028
    Keywords: wheat ; Triticum aestivum ; cDNA clone ; waxy protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016086
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    Structure and sequence of a wheat phosphoribulokinase gene (1991)
    Springer
    Plant molecular biology 17 (1991), S. 167-168 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; monocot gene ; phosphoribulokinase ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036823
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    Nucleotide sequence of a Triticum aestivum cDNA clone which is homologous to the 26 kDa chloroplast-localized heat shock protein gene of maize (1991)
    Springer
    Plant molecular biology 17 (1991), S. 255-258 
    Details
    ISSN: 1573-5028
    Keywords: Tahsp26.6 ; chloroplast-localized HSP ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039500
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    Analysis of nuclear proteins interacting with a wheat α/β-gliadin seed storage protein gene (1993)
    Springer
    Plant molecular biology 22 (1993), S. 25-41 
    Details
    ISSN: 1573-5028
    Keywords: gliadin storage proteins ; wheat ; transcriptional regulation ; nuclear proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The promoter region (−524 to −46) of the wheat α/β-gliadin seed storage protein gene was analyzed for interactions with nuclear proteins from developing wheat seeds. Six complexes were detected within the first 165 bp upstream of the transcriptional start site. One of the proteins was a non-sequence specific AT-binding protein. The remaining five proteins bound in a sequence specific manner. One (CABP) mapped to a conserved CA-rich element at −134 to −112 while another (PalBP) mapped to an adjacent, palindromic sequence at −112 to −106. Three proteins (CTBPs 1–3) formed complexes at two, independent homologous sites. The activities of four of the binding proteins, CTBPs 1–3 and CABP, exhibited similar patterns of expression during seed development: they first appeared at early to mid stages, reached a maximum at mid stage and subsequently decreased, paralleling the pattern of gliadin mRNA accumulation. The non-specific AT-binding protein was detected at relatively high levels only at mid development. PalBP activity, on the other hand, first appeared at mid stage and was present at a constant level throughout later stages of development. The results suggest that the binding proteins may regulate gliadin expression in an antagonistic manner.
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    URL: http://dx.doi.org/10.1007/BF00038993
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    A light- and developmentally-regulated DNA-binding interaction is common to the upstream sequences of the wheat Calvin cycle bisphosphatase genes (1993)
    Springer
    Plant molecular biology 22 (1993), S. 507-516 
    Details
    ISSN: 1573-5028
    Keywords: Calvin cycle genes ; DNA-binding proteins ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have characterised a DNA-binding interaction common to the upstream sequences of the wheat fructose-1,6-bisphosphatase (FBPase) and sedoheptulose-1,7-bisphosphatase (SBPase) genes. The recognition site for this sequence-specific binding activity, designated wheat FBPase factor (WF-1), is located within 125 bp of the transcription start site of each gene. Within these regions there are no sequence motifs similar to those shown to be important for light-regulated expression in other species. The binding activity was not detected in wheat root nuclear extracts, or in pea leaf extracts. There was a higher level of binding activity in light-grown than in dark-grown wheat leaves. The level was also found to decline when light-grown plants were given an extended dark treatment, but could be reinduced by light. Utilising the gradient of developmental maturity which exists within the wheat leaf it was found that WF-1 activity increases during leaf development.
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    URL: http://dx.doi.org/10.1007/BF00015979
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    Isolation and characterization of a cDNA clone encoding the TATA box-binding protein (TFIID) from wheat (1992)
    Springer
    Plant molecular biology 19 (1992), S. 867-872 
    Details
    ISSN: 1573-5028
    Keywords: TATA box ; TFIID ; transcription factor ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We isolated a complementary DNA (cDNA) encoding the TATA-binding factor ‘TFIID’ from a wheat seedling cDNA library. The wheat TFIID transcript of 1.2 kb poly(A)+ RNA was expressed at a low level early in germination, but gradually increased as the seedlings developed. In vitro binding experiments showed that the bacterially expressed wheat TFIID protein could specifically bind to the TATA boxes of the cauliflower mosaic virus (CaMV) 35S, wheat histone H3 and adenovirus major late genes with different affinity. A comparison with Arabidopsis TFIID showed the presence of a plant-specific region consisting of 13 amino acids at the divergent amino terminus and a conserved region (182 amino acids) at the carboxy terminus longer than that observed in yeasts (180 amino acids) and animals (181 amino acids).
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    URL: http://dx.doi.org/10.1007/BF00027083
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    Nucleotide sequence of the internal transcribed spacer region of rDNA in diploid wheat,Triticum speltoides L. (Tausch) Gren. ex Richter (Gramineae) (1992)
    Springer
    Plant molecular biology 20 (1992), S. 157-158 
    Details
    ISSN: 1573-5028
    Keywords: rRNA ; PCR ; ITS ; DNA sequence ; nucleotide ; Triticum speltoides ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00029158
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    Nucleotide sequence of the internal transcribed spacer region of rDNA in wheat,Triticum aestivum L. (Gramineae) (1992)
    Springer
    Plant molecular biology 20 (1992), S. 159-160 
    Details
    ISSN: 1573-5028
    Keywords: rRNA ; PCR ; ITS ; DNA sequence ; nucleotide ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029159
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    Sequence and organization of a 7.2 kb region of wheat mitochondrial DNA containing the large subunit (26S) rRNA gene (1992)
    Springer
    Plant molecular biology 20 (1992), S. 347-352 
    Details
    ISSN: 1573-5028
    Keywords: mitochondrial DNA ; repeated sequences ; ribosomal RNA ; t-elements ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report the sequence of a 7.2 kilobase pair DNA fragment containing a copy of the wheat mitochondrial gene (rrn26) that encodes the mitochondrial large-subunit ribosomal RNA (26S rRNA). The mature 26S rRNA was determined by direct RNA sequencing to be 3467 nucleotides long, and to share a 5′-terminal pentanucleotide (5′-AUCAU), thought to be important in post-transcriptional processing, with the wheat mitochondrial small-subunit (18S) rRNA. Two other prominent features of the sequence were noted. First, upstream of rrn26 are located two tandem copies of a 70 base pair element containing a putative mitochondrial promoter motif (TCGTATAAAAA). Second, downstream of rrn26 is a sequence element that, if transcribed, would produce and RNA with a secondary structure resembling that of tRNAs but differing sufficiently from the latter structure to preclude any transcript from functioning normally in translation. These upstream and downstream sequence elements may play a role in the expression of rrn26 in wheat mitochondria.
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    A ribosomal protein S10 gene is found in the mitochondrial genome in Solanum tuberosum (1994)
    Springer
    Plant molecular biology 25 (1994), S. 743-749 
    Details
    ISSN: 1573-5028
    Keywords: apocytochrome b pseudogene ; pea cox1 ; plant mitochondria ; potato ; S10 ribosomal protein ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The S10 ribosomal protein gene (rps10), which has not been previously reported in any angiosperm mitochondrial genome, was identified by sequence analysis in the potato mitochondrial DNA. This gene is found downstream of a truncated non-functional apocytochrome b (cob) pseudogene, and is expressed as multiple transcripts ranging in size from 0.8 to 5.0 kb. Southern hybridization analysis indicates that rps10-homologous sequences are not present in the wheat mitochondrial genome. Sequence analysis of a single-copy region of the pea mitochondrial genome located upstream of cox1 [11] shows that a non-functional rps10 pseudogene is present in this species. These results suggest that the functional genes coding for wheat and pea mitochondrial RPS10 polypeptides have been translocated to the nucleus.
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    URL: http://dx.doi.org/10.1007/BF00029612
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    Dynamical behavior of psb gene transcripts in greening wheat seedlings. I. Time course of accumulation of the psbA through psbN gene transcripts during light-induced greening (1992)
    Springer
    Plant molecular biology 20 (1992), S. 695-704 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; gene expression ; photosystem 2 ; transcription ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The time course of the accumulation of the transcripts from 13 psb genes encoding a major part of the proteins composing photosystem II during light-induced greening of dark-grown wheat seedlings was examined focusing on early stages of plastid development (0.5 h through 72 h). The 13 genes can be divided into three groups. (1) The psbA gene is transcribed as a single transcript of 1.3 kb in the dark-grown seedlings, but its level increases 5- to 7-fold in response to light due to selective increase in RNA stability as well as in transcription activity. (2) The psbE-F-L-J operon, psbM and psbN genes are transcribed as a single transcript of 1.1 kb, two transcripts of 0.5 and 0.7 kb and a single transcript of 0.3 kb, respectively, in the dark-grown seedlings. The levels of accumulation of every transcript remain unchanged or rather decrease during plastid development under illumination. (3) The psbK-I-D-C gene cluster and psbB-H operon exhibit fairly complicated northern hybridization patterns during the greening process. When a psbC or psbD gene probe was used for northern hybridization, five transcripts differing in length were detected in the etioplasts from 5-day old dark-grown seedlings. After 2 h illumination, two new transcripts of different length appeared. Light induction of new transcripts was also observed in the psbB-H operon.
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    Triticum aestivum puroindolines, two basic cystine-rich seed proteins: cDNA sequence analysis and developmental gene expression (1994)
    Springer
    Plant molecular biology 25 (1994), S. 43-57 
    Details
    ISSN: 1573-5028
    Keywords: cDNA sequence ; cystine-rich proteins ; gene expression ; puroindolines ; tryptophan-rich domain ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract From a mid-maturation seed cDNA library we have isolated cDNA clones encoding two Triticum aestivum puroindolines. Puroindoline-a and puroindoline-b, which are 55% similar, are basic, cystine-rich and tryptophan-rich proteins. Puroindolines are synthezised as preproproteins which include N- and C-terminal propeptides which could be involved in their vacuolar localization. The mature proteins have a molecular mass of 13 kDa and a calculated isoelectric point greater than 10. A notable feature of the primary structure of puroindolines is the presence of a tryptophan-rich domain which also contains basic residues. A similar tryptophan-rich domain was found within an oat seed protein and a mammalian antimicrobial peptide. The ten cysteine residues of puroindolines are organized in a cysteine skeleton which shows similarity to the cysteine skeleton of other wheat seed cystine-rich proteins. Northern blot analysis showed that puroindoline genes are specifically expressed in T. aestivum developing seeds. No puroindoline transcripts as well as no related genes were detected in Triticum durum. The identity of puroindolines to wheat starch-granule associated proteins is discussed as well as the potential role of puroindolines in the plant defence mechanism.
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    URL: http://dx.doi.org/10.1007/BF00024197
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    The wheat protein kinase gene, TaPK3/, of the PKABA1 subfamily is differentially regulated in greening wheat seedlings (1997)
    Springer
    Plant molecular biology 33 (1997), S. 935-941 
    Details
    ISSN: 1573-5028
    Keywords: cold temperature ; dehydration ; greening ; protein kinase ; Triticum aestivum/ ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have identified a new wheat PKABA1/-like protein kinase gene, TaPK3/, that is expressed in greening wheat seedlings. TaPK3 has high sequence homology (97% similarity with some sequence diversity at the 3' end) to the wheat PKABA1 protein kinase mRNA, which is upregulated by cold-temperature treatment, dehydration and abscisic acid (ABA). Use of a TaPK3 gene-specific probe has revealed that TaPK3 is differentially expressed with respect to PKABA1. TaPK3 mRNA accumulates in greening shoot tissue of wheat, but is not affected by dehydration, cold-temperature treatment or ABA. Based on sequence and expression differences, we conclude that expression of the PKABA1/-like protein kinases is not limited to stress responses.
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    URL: http://dx.doi.org/10.1023/A:1005720203535
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    cDNA structure and expression patterns of a low-temperature-specific wheat gene tacr7 (1997)
    Springer
    Plant molecular biology 34 (1997), S. 643-650 
    Details
    ISSN: 1573-5028
    Keywords: callus ; crown tissue ; gene expression ; low temperature ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The low-temperature (2 °C)-specific wheat cDNA, pTACR7, represents a gene designated tacr7 from hard red winter wheat (HRWW; Triticum aestivum L. cv. Winoka). The term low-temperature-specific (LTS) is used because tacr7 is not induced by ABA or stresses such as salt, dehydration, and heat. pTACR7 was isolated by RT-PCR with mRNA from wheat crown tissue, the oligonucleotide primers derived from the barley cognate pHVCR8 (GenBank accession number L28091). Based on the deduced amino acid sequence, TACR7 is highly hydrophobic, with a single transmembrane domain and an amino acid bias for leucine (19%). Thus, the encoded protein TACR7 is unique among low-temperature-regulated wheat proteins described in the literature. Analysis of steady-state levels of tacr7 transcripts (630 nt) showed accumulation in wheat seedlings, crown tissue, and callus cultures after transfer from control (25 °C) to low temperature (2 °C). No detectable transcripts were observed by northern blot hybridization with pTACR7 probe from seedling or callus treated with ABA, salt, dehydration, or heat stress. tacr7 transcripts accumulated during 2 °C exposure to a greater amount in a freeze-resistant HRWW (FR; SDmut 16029) than in a freeze-susceptible HRWW (FS; SDmut 16169) crown tissue, with the largest difference between genotypes being 30% ± 3% at 3 weeks.
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    URL: http://dx.doi.org/10.1023/A:1005852703506
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    Accelerated production of transgenic wheat (Triticum aestivum L.) plants (1996)
    Springer
    Plant cell reports 16 (1996), S. 12-17 
    Details
    ISSN: 1432-203X
    Keywords: Triticum aestivum L. ; wheat ; transformation ; biolistics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed a method for the accelerated production of fertile transgenic wheat (Triticum aestivum L.) that yields rooted plants ready for transfer to soil in 8–9 weeks (56–66 days) after the initiation of cultures. This was made possible by improvements in the procedures used for culture, bombardment, and selection. Cultured immature embryos were given a 4–6 h pre-and 16 h post-bombardment osmotic treatment. The most consistent and satisfactory results were obtained with 30 μg of gold particles/bombardment. No clear correlation was found between the frequencies of transient expression and stable transformation. The highest rates of regeneration and transformation were obtained when callus formation after bombardment was limited to two weeks in the dark, with or without selection, followed by selection during regeneration under light. Selection with bialaphos, and not phosphinothricin, yielded more vigorously growing transformed plantlets. The elongation of dark green plantlets in the presence of 4–5 mg/l bialaphos was found to be reliable for identifying transformed plants. Eighty independent transgenic wheat lines were produced in this study. Under optimum conditions, 32 transformed wheat plants were obtained from 2100 immature embryos in 56–66 days, making it possible to obtain R3 homozygous plants in less than a year.
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    The genetical control of tissue-specific peroxidases,Per-1,Per-2,Per-3,Per-4, andPer-5 in wheat (1990)
    Springer
    Theoretical and applied genetics 79 (1990), S. 305-313 
    Details
    ISSN: 1432-2242
    Keywords: Peroxidase ; Isoelectric focusing ; Hexaploid ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Isoelectric focusing (IEF) of extracts from different tissues of hexaploid wheat cv “Chinese Spring” provided a method of distinguishing and identifying the four known, and one newly discovered, sets of genes encoding peroxidase isozyme production.Per-1, carried on the short arms of homoeologous group 1 chromosomes, shows a high degree of conservation and is active in coleoptile tissue.Per-2, carried on the short arms of group 2 chromosomes, shows some polymorphism and is most active in root tissue.Per-3, on the long arms of group 3 chromosomes, is highly variable and most active in embryo tissue.Per-4, carried on chromosome arms7AS,4AL, and7DS, is quite variable and most active in endosperm tissue. (The chromosome nomenclature used in this paper is that agreed to by the 7th International Wheat Genetics Symposium, where the previous designations of4A and4B were reversed.) Restriction fragment length polymorphism (RFLP)-based maps of the group 7 chromosomes were used to locatePer-A4 to a distal region of7AS. In addition, a further set of genes was identified as being active in root tissue. In wheat a single locus,Per-D5, was found on chromosome arm2DS.
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    Chromosomal location by F1 monosomic analysis of endosperm proteins in bread wheat (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 933-940 
    Details
    ISSN: 1432-2242
    Keywords: Gliadin ; Two-dimensional electrophoresis ; F1 monosomic analysis ; chromosomal assignment ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gliadin components from four bread wheat cultivars: Chinese Spring, Capelle Desprez, Holdfast and Pane-247 and their monosomic F1s for the chromosomes of homoeologous groups 1 and 6 have been analyzed by two-dimensional (2-pH) polyacrylamide gel electrophoresis. Chromosomal location of gliadin genes and the allelic differences were well established by analyzing the different F1 monosomic hybrids, electrophoretical patterns and differences in relative staining intensity. A new gliadin encoded by a gene located on chromosome 6B in Chinese Spring is described. The two-dimensional patterns of gliadin in the other three varieties and the chromosomal location of their genes are reported for the first time. Relationships between gliadins in the two-dimensional patterns and the traditional system for their nomenclature are discussed.
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    Inferred chromosome morphology of the ancestral genome ofTriticum (1984)
    Springer
    Plant systematics and evolution 144 (1984), S. 209-220 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticum ; Elytrigia ; wheat ; Evolution ; genome ; karyotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lengths of the A, B, and D genomes of common wheat,Triticum aestivum, were measured from the karyotype. Relative to the B genome, standardized as length 1.000, the lengths of the A and D genomes were 0.835 and 0.722, respectively. The lengths of the chromosome arms in the A and D genomes were then multiplied by the appropriate constants so that the total lengths of each genome also equalled 1.000. These calculations revealed that homoeologous chromosomes in wheat, with a few exceptions, have similar sizes and arm ratios. The arm lengths of the three homoeologues in each homoeologous group were then averaged. These average chromosomes turned out to be remarkably similar, in size and arm ratio, to their homoeologues in the E genome ofElytrigia elongata. This evidence and data on cross-compatibility and morphological characteristics suggested that the genusTriticum is a result of adaptive radiation from the perennial genusElytrigia, specifically from the complex of species possessing the E genome or one closely related to it.
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    Growth promotion ability of zoysiagrass rhizosphere fungi in consecutive plantings of wheat and soybean (1996)
    Springer
    Mycoscience 37 (1996), S. 163-168 
    Details
    ISSN: 1618-2545
    Keywords: consecutive planting ; Phoma sp. ; plant growth-promotion ; potting medium ; soybean ; wheat ; zoysiagrass rhizosphere fungi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several isolates ofPhoma sp., certain nonsporulating fungi, as well asPenicillium andTrichoderma, all isolated from zoysiagrass rhizosphere, promoted growth of wheat and soybean under greenhouse conditions. However, the ability of these rhizosphere fungi to enhance plant growth varied with the crop tested. For example, most of the fungi effectively promoted the growth of wheat, whereas only a few fungi were effective on soybean. In consecutive plantings of wheat and soybean grown in soil previously infested with these zoysiagrass rhizosphere fungi, the growth promotion ability of the fungi was lowered. However, addition of fresh potting medium appeared to restore their growth-promotive effects. It appears that the activation of plant growth-promoting fungi in soil might depend on the availability of organic substrates to colonize, as evidenced by the promotion of plant growth.
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    URL: http://dx.doi.org/10.1007/BF02461341
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    Mapping loci associated with milling yield in wheat (Triticum aestivum L.) (1999)
    Springer
    Molecular breeding 5 (1999), S. 561-568 
    Details
    ISSN: 1572-9788
    Keywords: wheat ; milling yield ; QTL mapping ; RFLP ; microsatellite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A partial genetic linkage map constructed using 150 single seed descent (SSD) lines generated from a cross between the hexaploid wheat varieties ‘Schomburgk’ and ‘Yarralinka’ was used to identify loci controlling milling yield. Milling yield data were obtained using seed collected from field trials conducted at different sites over two seasons. The estimated broad-sense heritability of milling yield in this population was calculated as 0.48. In the preliminary analysis, two regions were identified on chromosomes 3A and 7D, which were significantly associated with milling yield and accounted for 22% and 19% of the genetic variation, respectively. Bulked segregant analysis in combination with AFLP identified other markers linked to these loci, as well as an additional region on chromosome 5A, which accounted for 19% of the genetic variation. The applicability of these markers as selection tools for breeding purposes is discussed.
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    Development of a STS marker linked to a major locus controlling flour colour in wheat (Triticum aestivum L.) (2000)
    Springer
    Molecular breeding 6 (2000), S. 169-174 
    Details
    ISSN: 1572-9788
    Keywords: AFLP ; flour colour ; STS ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Flour colour is an important quality trait in the production of bread, noodles and other related end products. Current screening for flour colour in breeding programs requires several grams of flour to be milled. In order to screen large numbers of plants, a rapid PCR-based assay is required. We report here the conversion of a codominant AFLP marker linked to a major locus controlling flour colour in hexaploid wheat, to a sequence tagged site (STS) marker for use in marker-assisted selection (MAS). The two-allelic AFLP bands were cloned and sequenced to allow specific primers to be designed. The primers amplified bands of the expected size in the parental varieties and co-segregated with the original AFLP marker in the mapping population. The primers also amplified alleles of the expected size from the DNA of parental lines of two other related mapping populations. Cultivars that contributed to the pedigree of the original parent `Schomburgk' used to generate the mapping population were also screened to determine the origin of the `yellow' allele.
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    URL: http://dx.doi.org/10.1023/A:1009638017859
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    Expression of the insecticidal lectin from snowdrop (Galanthus nivalis agglutinin; GNA) in transgenic wheat plants: effects on predation by the grain aphid Sitobion avenae (1999)
    Springer
    Molecular breeding 5 (1999), S. 65-73 
    Details
    ISSN: 1572-9788
    Keywords: insect resistance ; aphids ; GNA ; lectins ; transgenic plants ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Transgenic wheat plants containing the gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under the control of constitutive and phloem-specific promoters were generated through the particle bombardment method. Thirty-two independently derived plants were subjected to molecular and biochemical analyses. Transgene integration varied from one to twelve estimated copies per haploid genome, and levels of GNA expression from 0 to ca. 0.2% of total soluble protein were observed in different transgenic plants. Seven transgenic plants were selected for further study. Progeny plants from these parental transformants were selected for transgene expression, and tested for enhanced resistance to the grain aphid (Sitobion avenae) by exposing the plants to nymphal insects under glasshouse conditions. Bioassay results show that transgenic wheat plants from lines expressing GNA at levels greater than ca. 0.04% of total soluble protein decrease the fecundity, but not the survival, of grain aphids. We propose that transgenic approaches using insecticidal genes such as gna in combination with integrated pest management present promising opportunities for the control of damaging wheat pests.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009616413886
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    Identification of molecular markers for the detection of the yellow rust resistance gene Yr17 in wheat (1999)
    Springer
    Molecular breeding 5 (1999), S. 167-175 
    Details
    ISSN: 1572-9788
    Keywords: wheat ; DNA markers ; yellow rust resistance ; Yr17
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The Yr17 gene, which is present in many European wheat cultivars, displays yellow rust resistance at the seedling stage. The gene introduced into chromosome 2A from Aegilops ventricosa was previously found to be closely linked (0.5 cM) to leaf and stem rust resistance genes Lr37 and Sr38, respectively. The objective of this study was to identify molecular markers linked to the Yr17 gene. We screened with RAPD primers, for polymorphism, the DNAs of cv. Thatcher and the leaf rust-resistant near-isogenic line (NIL) RL 6081 of cv. Thatcher carrying the Lr37 gene. Using a F2 progeny of the cross between VPM1 (resistant) and Thésée (susceptible), the RAPD marker OP-Y15580 was found to be closely linked to the Yr17 gene. We converted the OP- Y15580 RAPD marker into a sequence characterized amplified region (SCAR). This SCAR marker (SC-Y15) was linked at 0.8 ± 0.7 cM to the Yr17 resistance gene. We tested the SC-Y15 marker over a survey of 37 wheat cultivars in order to verify its consistency in different genetic backgrounds and to explain the resistance of some cultivars against yellow rust. Moreover, we showed that the Xpsr150-2Mv locus marker of Lr gene described by Bonhomme et al. [6] which possesses A. ventricosa introgression on the 2A chromosome was also closely linked to the Yr17 gene. Both the SCAR SC-Y15 and Xpsr150-2Mv markers should be used in breeding programmes in order to detect the cluster of the three genes Yr17, Lr37 and Sr38 in cross progenies.
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    URL: http://dx.doi.org/10.1023/A:1009672021411
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    Molecular markers for the detection of the wheat leaf rust resistance gene Lr10 in diverse genetic backgrounds (1997)
    Springer
    Molecular breeding 3 (1997), S. 65-74 
    Details
    ISSN: 1572-9788
    Keywords: leaf rust ; molecular marker ; receptor-like kinase ; resistance breeding ; resistance gene ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We recently showed that the Lr10 wheat leaf rust resistance gene cosegregated with the candidate resistance gene Lrk10 which encodes a putative receptor-like kinase. The aim of this study was to develop Lrk10-derived molecular markers for the detection of the Lr10 gene in breeding material. Different subfragments of Lrk10 were tested as RFLP markers for the Lr10 resistance gene. The most specific fragment (Lrk10-6) was converted into the PCR-based STS marker STSLrk10-6. Both the RFLP and the STS marker did not give a signal with near isogenic lines containing a different Lr gene. The applicability of these markers for the detection of Lr10 in genetically diverse material was tested with 62 wheat and spelt breeding lines, mostly from European breeding programmes. Twelve varieties known to have Lr10 showed the same alleles as the originally characterized line ThatcherLr10. Most of the lines with unknown composition at the Lr10 locus had a null allele with both the RFLP marker Lrk10-6 and the marker STSLrk10-6 whereas 20% of the lines had a different allele. For six lines, including a traditional spelt variety derived from a landrace, both markers showed the same allele as Thatcher Lr10. Artificial infections of these lines with an isolate avirulent on Lr10 resulted in a hypersensitive reaction of all these lines, indicating also the presence of the Lr10 resistance gene. These data demonstrate that the markers derived from sequences of Lrk10 are highly specific for the Lr10 gene in breeding material of very diverse genetic origin. The markers will allow the defined deployment of Lr10 in wheat breeding programmes and will contribute to the elucidation of the role of Lr10 in polygenic resistances against leaf rust.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009619905909
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    A molecular marker associated with the H21 Hessian fly resistance gene in wheat (1997)
    Springer
    Molecular breeding 3 (1997), S. 177-181 
    Details
    ISSN: 1572-9788
    Keywords: bulked segregant analysis ; H21 ; near-isogenic lines (NILs) ; RAPD ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Near-isogenic lines in conjunction with bulked segregant analysis were used to identify a DNA marker in wheat (Triticum aestivum L.) associated with the H21 gene conferring resistance to biotype L of Hessian fly [Mayetiola destructor (Say)] larvae. Near-isogenic lines were developed by backcross introgression BC3F3:4 (‘Coker 797’ * 4 / ‘Hamlet’) and differed by the presence or absence of H21 (on 2RL) derived from ‘Chaupon’ rye (Secale cereale L.). Bulked DNA samples were prepared from near-isogenic lines and BC3F2 population individuals segregating for reaction to Hessian fly biotype L and screened for random amplified polymorphic DNA markers using 46 10mer primers. Random-amplified polymorphic DNA markers from resistant and susceptible individuals and parental lines were scored and these data were used to identify a 3 kb DNA fragment that was related to the occurrence of H21. This fragment was amplified from DNA isolated from Hamlet, a near-isogenic line carrying 2RL, and bulked DNA from resistant BC3F2 individuals, but not from the recurrent parent Coker 797 or DNA bulks from susceptible BC3F2 plants. Analysis of 111 BC3F2 segregating individuals and BC3F2:3 segregants confirmed the co-segregation of the 3 kb DNA marker with the H21 resistance gene to Hessian fly. Use of this marker could facilitate more rapid screening of plant populations for Hessian fly resistance and monitoring the introgression of H21.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009606304447
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    Structure of populations of wheat powdery mildew (Erysiphe graminis DC f.sp. tritici Marchal) in Central Europe in 1993–1996: I. Dynamics of virulence (1998)
    Springer
    European journal of plant pathology 104 (1998), S. 537-544 
    Details
    ISSN: 1573-8469
    Keywords: Central Europe ; powdery mildew ; wheat ; complexity of pathotypes ; Erysiphe graminis f.sp. tritici ; stabilizing selection ; survey ; virulence analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In 1993–1996, the virulence of regional populations of the wheat powdery mildew pathogen (Erysiphe graminis DC f. sp. tritici Marchal) from the Czech Republic, Austria, Hungary and Slovakia against 13 resistance genes was investigated. The populations differed mainly at the regional level. Populations from the Czech Republic, mainly from the western regions, showed higher values of virulence against the Pm4b gene. Lower frequency of virulence against Pm4b was found in Austria, and the lowest value was observed in Hungary. The differences in frequencies of virulence against Pm4a and Pm4b showed a similar geographic pattern across the four countries: a continuous decline from west to east and from north to south. Virulence against Pm2 decreased in all countries considered; virulence to pm5, Pm6, Pm8 and Mli was high throughout. Genes and gene combinations that can ensure a relatively effective biological protection against this pathogen across Central Europe at present are Pm3b, Pm2+Mld and Pm1+2+9. Czech and Slovak populations were the most complex: virulence complexity reached a maximum in Slovakia in 1994. A similar evolution, though less significant, was observed in the Czech Republic. Data on complexity of isolates suggest that Central European populations of wheat powdery mildew tend to reach an intermediate level representing the optimal number of virulence genes. This process is probably a consequence of stabilizing selection.
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    URL: http://dx.doi.org/10.1023/A:1008642816326
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    The Septoria Tritici and Stagonospora Nodorum Blotch Diseases of Wheat (1999)
    Springer
    European journal of plant pathology 105 (1999), S. 629-641 
    Details
    ISSN: 1573-8469
    Keywords: wheat ; Triticum spp. ; Septoria tritici ; septoria tritici blotch of wheat Stagonospora nodorum ; stagonospora nodorum blotch of wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008716812259
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    Disease levels in winter wheat, rye and triticale grown on soil artificially inoculated withCephalosporium gramineum (1995)
    Springer
    European journal of plant pathology 101 (1995), S. 701-704 
    Details
    ISSN: 1573-8469
    Keywords: Cephalosporium gramineum ; Cephalosporium stripe ; rye ; susceptibility ; triticale ; wheat ; winter cereals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Field experiments with winter cereals grown on soil inoculated withC. gramineum showed that wheat and rye cultivars possess some resistance to the pathogen, while the triticale cultivars were the most susceptible. Higher tolerance of the tested wheat cultivars was connected mainly with slow development of disease symptoms; rye cultivars had, on average, lower percentages of plants infected byC. gramineum. The greatest variation in susceptibility toC. gramineum occurred among the selected cultivars of triticale.
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    URL: http://dx.doi.org/10.1007/BF01874875
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    Development of apothecia ofTapesia yallundae in contrasting populations selected by fungicides (1995)
    Springer
    European journal of plant pathology 101 (1995), S. 695-699 
    Details
    ISSN: 1573-8469
    Keywords: Pseudocercosporella herpotrichoides ; eyespot ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Apothecia of the eyespot fungus,Tapesia yallundae, were found on 0–18% of straws in plots of wheat stubble in February–March 1994. The fungicides carbendazim, prochloraz or carbendazim plus prochloraz had been applied repeatedly to the same plots in each of the previous 9 years in which successive wheat crops had been grown. The factors most strongly correlated with the incidence of apothecia were the incidence and severity of eyespot in the preceding wheat crop and the frequency of carbendazim-resistant W-type fungus in populations recovered from that wheat crop. Plots treated with carbendazim, which had previously had more disease and more resistance to carbendazim in the pathogen population relative to untreated plots, therefore yielded most apothecia. Plots treated with prochloraz, which had selected for predominantly R-type fungus and decreased eyespot, yielded few apothecia. Single-ascospore isolates were all of the W-type and were more frequently carbendazim-sensitive than expected, except those from plots treated only with carbendazim. None showed decreased sensitivity to prochloraz. The implications of applying fungicides regularly for controlling eyespot on the capability of the eyespot fungus for genetic variation through sexual reproduction are discussed.
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    Meiosis of Wheat × Lymegrass Hybrids (1998)
    Springer
    Chromosome research 6 (1998), S. 339-344 
    Details
    ISSN: 1573-6849
    Keywords: Leymus ; meiosis ; molecular cytogenetics ; wheat ; wide-hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Meiosis was examined in pollen mother cells of F1 hybrids made from crosses between wheat (Triticum aestivum) and lymegrass (Leymus arenarius and L. mollis). Fluorescence genomic in situ hybridization detected pairing between wheat and lymegrass chromosomes during prophase I and metaphase I. Such pairing, when resulting in bivalent formation, was likely to yield correct disjunction, and hence intergenomic recombination could be incorporated into the gametes. Bivalents in these hybrids, however, were more frequently formed between chromosomes of the same parental origin. Univalents were common, whereas multivalents were not clearly detected. Meiotic behaviour in some cells was not totally aberrant, and this may have accounted for the presence of normal pollen. The results are discussed in relation to intergenomic pairing, meiotic behaviour in wide-hybrids and genome relationships, including the Leymus genome origin.
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    URL: http://dx.doi.org/10.1023/A:1009281911723
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    Characterization of the translocated chromosome using fluorescencein situ hybridization and random amplified polymorphic DNA on twoTriticum aestivum—Thinopyrum intermedium translocation lines resistant to wheat streak mosaic or barley yellow dwarf virus (1996)
    Springer
    Chromosome research 4 (1996), S. 583-587 
    Details
    ISSN: 1573-6849
    Keywords: in situ hybridization ; intermediate wheatgrass ; translocation ; virus resistance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluorescencein situ hybridization (FISH) was used to determine the breakpoint of the translocation chromosome in two bread wheat (Triticum aestivum) germplasm lines withThinopyrum intermedium chromatin carrying resistance to either wheat streak mosaic virus (WSMV) or barley yellow dwarf virus (BYDV). In addition, genome-specific random amplified polymorphic DNA (RAPD) markers were used to ascertain the genomic sources of theTh. intermedium chromosomes carrying the WSMV or BYDV resistance. CI17766, a WSMV-resistant wheat germplasm line derived from induced homoeologous pairing by using theph1b mutant, had a translocation chromosome composed of the complete 4AL and about 45% of proximal 4AS from wheat, and the entire 4ES ofTh. intermedium. The BYDV-resistant translocation line, TC14, derived from tissue culture, had a very short distal segment of 7StL fromTh. intermedium terminally attached to 56% of the proximal 7DL. These observations indicate that translocations in these wheat germplasm lines did not involve centromeric breaks and fusion but were a result of homoeologous chromosome recombination.
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    URL: http://dx.doi.org/10.1007/BF02261721
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    Comparative genetics of flowering time (1997)
    Springer
    Plant molecular biology 35 (1997), S. 167-177 
    Details
    ISSN: 1573-5028
    Keywords: comparative genetics ; rice ; barley ; wheat ; Arabidopsis ; flowering time (heading date) ; photoperiod ; vernalization ; earliness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of genes controlling flowering time (heading date) contributes to our understanding of fundamental principles of plant development and is of practical importance because of the effects of flowering time on plant adaptation and crop yield. This review discusses the extent to which plants may share common genetic mechanisms for the control of flowering time and the implications of such conservation for gene isolation from the major cereal crops. Gene isolation may exploit the small genome of rice in map-based approaches, utilizing the conservation of gene order that is revealed when common DNA markers are mapped in different species. Alternatively, mechanisms may be conserved within plants as a whole, in which case genes cloned from the model dicot Arabidopsis thaliana provide an alternative route.
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    URL: http://dx.doi.org/10.1023/A:1005726329248
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    The significance of the magnesium to manganese ratio in plant tissues for growth and alleviation of manganese toxicity in tomato (Lycopersicon esculentum) and wheat (Triticum aestivum) plants (1990)
    Springer
    Plant and soil 124 (1990), S. 205-210 
    Details
    ISSN: 1573-5036
    Keywords: Lycopersicon esculentum L. ; magnesium-manganese interaction ; magnesium: manganese ratio ; manganese toxicity ; tomato ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Results are reported for tomato (Lycopersicon esculentum L. var. Ailsa craig) and wheat (Triticum aestivum L. cv. Mara) which demonstrate that increasing concentrations of Mg in the plant raises plant tolerance to Mn toxicity. Water culture experiments with tomato show that under conditions of high Mn supply (200 µM, Mn), not only does increasing Mg application (0.75 mM to 15 mM) depress Mn uptake, but the higher Mg concentrations in the shoot counteract the onset of Mn toxicity when the concentrations of Mn in the shoot are also high. The ratio of Mg: Mn in the tissues is a better indicator of the appearance of toxicity symptoms than Mn concentration alone. Toxicity symptoms were observed when the Mg:Mn ratio in the shoot tissue was from 1.13 to a value between 3.53 and 6.54. The corresponding Mg: Mn ratio in the older leaves was from 0.82 to between 2.27 and 3.51. For wheat grown in soil, analyses of leaves revealed that growth could be expressed by the following relationship: Y=A+B exp(-kX), where Y=growth, X=Mg:Mn ratio, A, B and k=constants. Growth was significantly reduced when the Mg:Mn ratio fell below 20:1. From a measurement of this ratio it is therefore possible to predict the appearance of Mn toxicity and its influence on growth.
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    Nitrogen mineralization in a green manure-amended soil as influenced by cropping history and subsequent crop (1989)
    Springer
    Plant and soil 120 (1989), S. 125-131 
    Details
    ISSN: 1573-5036
    Keywords: canola ; fallow ; N mineralization ; N immobilization ; organic matter ; rhizosphere ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A greenhouse experiment was conducted to determine the influence of cropping variables on nitrogen dynamics in a soil amended with green manure. Surface soil from various long-term spring wheat rotations was amended with15N-labelled legume green manure (Lathyrus tingitanus) and subsequently cropped (canola [Brassica napus] and spring wheat [Triticum aestivum]) or incubated without a crop for 56 days in a greenhouse. Nitrogen mineralization from both the indigenous soil N and from green manure was suppressed in cropped soil. Net N mineralization in the uncropped and cropped treatments averaged 73 and 43 mg kg−1, respectively. This difference was attributed, in part, to enhanced biological immobilization in the rhizosphere. Previous cropping practices also had significant effect on N mineralization, largely by their influence on indigenous organic matter quality. These observations suggest that short-term N mineralization is favored by fallowing soil after green manure application whereas N retention in organic matter is favored by immediate cropping.
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    Molecular characterization of a new type of receptor-like kinase (wlrk) gene family in wheat (1998)
    Springer
    Plant molecular biology 37 (1998), S. 943-953 
    Details
    ISSN: 1573-5028
    Keywords: barley ; kinase ; leaf rust ; receptor-like kinase ; resistance ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In plants, several types of receptor-like kinases (RLK) have been isolated and characterized based on the sequence of their extracellular domains. Some of these RLKs have been demonstrated to be involved in plant development or in the reaction to environmental signals. Here, we describe a RLK gene family in wheat (wlrk, wheat leaf rust kinase) with a new type of extracellular domain. A member of this new gene family has previously been shown to cosegregate with the leaf rust resistance gene Lr10. The diversity of the wlrk gene family was studied by cloning the extracellular domain of different members of the family. Sequence comparisons demonstrated that the extracellular domain consists of three very conserved regions interrupted by three variable regions. Linkage analysis indicated that the wlrk genes are specifically located on chromosome group 1 in wheat and on the corresponding chromosomes of other members of the Triticeae family. The wlrk genes are constitutively expressed in the aerial parts of the plant whereas no expression was detected in roots. Protein immunoblots demonstrated that the WLRK protein coded by the Lrk10 gene is an intrinsic plasma membrane protein. This is consistent with the hypothesis that WLRK proteins are receptor protein kinases localized to the cell surface. In addition, we present preliminary evidence that other disease resistance loci in wheat contain genes which are related to wlrk.
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    Characterization of a toxin produced by a rhizobacterialPseudomonas sp. that inhibits wheat growth (1989)
    Springer
    Plant and soil 114 (1989), S. 279-287 
    Details
    ISSN: 1573-5036
    Keywords: characterization ; deleterious rhizobacteria ; inhibitory bacteria ; pseudomonads ; toxin ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A toxin produced by a deleterious rhizobacterial pseudomonad that inhibits both winter wheat (Triticum aestivum L.) root andEscherichia coli growth was characterized. The toxin was rapidly deactivated at pH 2 and 12 and by autoclaving (121°C, 15 minutes). Less toxin was destroyed as the temperature and time of exposure decreased, and at 40°C it was stable for at least 24 hours. The toxin was extremely polar and could not be extracted from culture filtrates with organic solvents. The compound eluted after the void volume from a Sephadex G-10 column indicating a molecular weight of less than 700. The toxin adsorbed to Dowex 50W strong cation exchange resin and eluted with 2M NH4OH. Numerous thin layer chromatography solvent systems were unsuccessful at purifying the toxin. The partially purified toxin inhibited several different microorganisms while the producing strains were resistant. The toxin appears unique to toxins produced by recognized plant pathogenic bacteria.
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    Influence of potassium nutrition on concentrations of water soluble carbohydrates, potassium, calcium, and magnesium and the osmotic potential in sap extracted from wheat (Triticum aestivum) ears during preanthesis development (1990)
    Springer
    Plant and soil 124 (1990), S. 281-285 
    Details
    ISSN: 1573-5036
    Keywords: calcium ; ear development ; magnesium ; potassium ; tissue press sap ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract For floret development and final grain number ear elongation in wheat (10–17d before anthesis) is the most sensitive period to adverse growth conditions. Sugars are supposedly the main factor determining floret numbers and grain set, and play next to potassium a significant role in establishing turgor pressure in young tissues. In view of this osmotic function, the influence of K on the concentration of water soluble carbohydrates (WSC, total of sucrose and reducing sugars), Ca and Mg was investigated in pot experiments. Further, the osmotic potential of sap extracted from young ears was determined and compared to sap extracted from the leaf blade. Plants supplied with low amounts of K (moderate K-deficiency) had a considerably lower K concentration in the press sap of the flag leaf and the ear than plants well supplied with K. Concentrations of WSC, Ca and Mg were higher in press sap of the flag leaf in K deficient plants than in plants adequately supplied with K. This indicates a substitution of K in its osmotic role. In press sap from ears, however, WSC, Ca and Mg were not influenced by the K application. Therefore, substances other than those measured must have been responsible for the osmoregulation in the young ear. WSC and the osmotic potential increased (more negative) independently on K supply during ear elongation, while K, Ca and Mg concentrations decreased. Whereas grain number was not influenced by the treatment, single grain weight at maturity was reduced by low K availability in the soil.
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    URL: http://dx.doi.org/10.1007/BF00009273
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    Modelling potassium uptake by wheat (Triticum aestivum) crops (1990)
    Springer
    Plant and soil 124 (1990), S. 303-307 
    Details
    ISSN: 1573-5036
    Keywords: models ; potassium uptake ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spring wheat was grown in the field under deficient and sufficient levels of soil K and with high and low supplies of fertiliser nitrogen. Measurements were made of K uptake, soil nutrient supply parameters, root growth and, in solution culture, root influx parameters. Mechanistic models predicted uptake reasonably well under K-deficient conditions, but over-predicted uptake, by as much as 4 times, under K-sufficient conditions. The over-prediction was apparently due to poor characterisation of plant demand.
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    URL: http://dx.doi.org/10.1007/BF00009277
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    Effects of nitrogen sources and copper levels on yield, nitrogen and copper contents of wheat (Triticum aestivum L.) (1990)
    Springer
    Plant and soil 126 (1990), S. 79-83 
    Details
    ISSN: 1573-5036
    Keywords: Cu levels ; N sources ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A pot experiment was conducted, in a greenhouse, at Hisar, India, using a sandy soil deficient in nitrogen and copper, to study the effects of various levels of N and Cu on the dry matter yield and the N and Cu contents of wheat (Triticum aestivum L.). The sources of nitrogen used were Ca(NO3)2, NH4Cl and NH4NO3 applied in amounts necessary to establish 120 ppm of soil nitrogen and using a control (0 ppm N). Copper was applied, as copper chloride, to give soil Cu levels of 0, 5, 10 and 20 ppm. In general, dry matter yields, N and Cu concentrations in shoots and roots and available soil-N after harvest of the plants, followed the order Ca(NO3)〉NH4NO3〉NH4Cl. Up to a level of 5 ppm Cu, the dry matter yields of shoots and roots increased, but decreased at higher levels of Cu. Increasing Cu levels significantly decreased the available soil-nitrogen after harvest and also the concentration of N in the plants. At the same time the concentration of Cu in shoots and roots and available Cu in the soil was increased. Nitrogen and copper were found to have a mutually antagonistic effect on each other's concentration in the plants. The antagonism was greater with NH4 + sources than with NO3 − compounds.
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    URL: http://dx.doi.org/10.1007/BF00041371
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    Purified Ribulose-P2 carboxylase from wheat with high specific activity and with fast activation (1984)
    Springer
    Photosynthesis research 5 (1984), S. 47-62 
    Details
    ISSN: 1573-5079
    Keywords: ribulose-P2 ; carboxylase ; wheat ; high specific activity ; fast activation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018374
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    Effects of three microbial broth cultures and an organic amendment on growth and populations of free living and plant-parasitic nematodes on banana (1998)
    Springer
    European journal of plant pathology 104 (1998), S. 457-463 
    Details
    ISSN: 1573-8469
    Keywords: Bacillus thuringiensis ; biological control ; Helicotylenchus multicinctus ; nematicide ; numerical response ; Paecilomyces marquandii ; Radopholus similis ; Streptomyces costaricanus ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of 24 treatment combinations of cultures of Streptomyces costaricanus sp. nov. (ATCC55274), Bacillus thuringiensis (ATCC55273) and a strain of Paecilomyces marquandii, nematicide (cadusaphos), and/or wheat mash on growth and response of potted banana plants (Musa AAA) and populations of Radopholus similis, Helicotylenchus multicinctus and free living nematodes were studied in Río Frío, Costa Rica. The best plant responses (height, leaf numbers, healthy root weight), lowest numbers of plant parasitic nematodes and highest numbers of free living nematodes were observed for treatments containing wheat as a component. Two treatments, viz. wheat + Streptomyces costaricanus (200-ml culture) and wheat + P. marquandii (200-ml culture), gave the overall best results. Numbers of free living nematodes increased up to 1500-fold only for treatments containing wheat. Significant positive correlations existed between numbers of free living nematodes and shoot weight, healthy root biomass, plant height, and leaf numbers. Non-wheat treatments, including nematicide only, gave the poorest responses in general. Observations of nematodes sampled 50 days following planting in wheat-containing treatments showed most of the free-living nematodes (≈ 90%) to be infected by nematophagous fungi (species not recorded). The results show that an organic amendment to soil, with or without a microbial component, can be an effective inducer of processes that regulate plant-parasitic nematode populations in soil.
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    URL: http://dx.doi.org/10.1023/A:1008638419552
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    Evaluation of various methods for the detection of barley yellow dwarf virus by the tissue-blot immunoassay and its use for virus detection in cereals inoculated at different growth stages (1994)
    Springer
    European journal of plant pathology 100 (1994), S. 71-80 
    Details
    ISSN: 1573-8469
    Keywords: wheat ; Triticum aestivum ; oat ; Avena sativa ; barley ; Hordeum vulgare ; serology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Various modifications of the tissue-blot immunoassay (TBIA) for the detection of barley yellow dwarf virus (BYDV, luteovirus) were compared. Similar results were obtained by using three different labelled molecules; goat anti-rabbit antibodies conjugated to alkaline phosphatase, protein A conjugated with alkaline phosphatase and goat anti-rabbit antibodies conjugated with colloidal gold. Blocking the nitrocellulose membrane with polyvinyl alcohol for 1 min was effective and allowed the procedure to be shortened by one hour. TBIA was sensitive enough to detect BYDV in old dry tissue wich had been soaked in water for 1 h. BYDV was monitored by TBIA in wheat, oat and barley after inoculation at heading, flowering and grain filling growth stages. The later the inoculation date, the greater the chance of detecting the virus in stem bases rather than in the upper part of the stem. The later the inoculation the less virus moved, from the inoculated tiller to other tillers of the same plant.
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    URL: http://dx.doi.org/10.1007/BF01871967
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    Fusarium head blight and mycotoxin contamination of wheat, a review (1990)
    Springer
    European journal of plant pathology 96 (1990), S. 187-198 
    Details
    ISSN: 1573-8469
    Keywords: fusarium head blight ; Fusarium culmorum ; F. graminearum ; wheat ; mycotoxin ; deoxynivalenol ; nivalenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Aaraantasting van tarwe doorFusarium culmorum enFusarium graminearum leidt tot vorming van mycotoxinen in het graan, waarvan deoxynivalenol (DON) en nivalenol (NIV) de belangrijkste toxinen zijn. In dit artikel wordt een overzicht gegeven van de toxicologische aspecten, en het voorkomen van deze toxinen in tarwe. Informatie over DON en NIV in tarwe in West-Europa is schaars. Gebaseerd op gegevens vanFusarium epidemieën in de jaren 1979–1986 wordt een schatting gegeven van de concentratie DON in Nederlandse tarwe. Rekening houdend met de herkomst en verwerking van tarwe, blijken zowel in dierlijk als menselijk voedsel lage concentraties DON chronisch voor te komen. Op basis van een maximaal toelaatbare dagelijkse dosis DON van 3 μg kg−1 lichaamsgewicht is de schatting van de dagelijkse opname van DON in het jaar volgend op de oogst van 1982 net op de grens. Zowel een jaarlijkse inventarisatie vanFusarium aantasting en DON besmetting van het graan, als de ontwikkeling vanFusarium-resistente rassen zijn noodzakelijk.
    Notes: Summary An infection of bread wheat by fusarium head blight contaminates the crop with mycotoxins, particularly deoxynivalenol (DON) and nivalenol (NIV). The toxicity and natural occurrence of these mycotoxins in wheat are reviewed. Based on 8 years data of fusarium head blight epidemics of wheat in the Netherlands, DON contamination of the grain was estimated. Fusarium head blight ratings averaged an infection of 1.7% of all spikelets; estimates for DON contamination averaged 0.9 mg kg−1. Taking a guideline level for DON in uncleaned bread wheat of 2 mg kg−1, in 1979 and 1982 a wheat crop was produced with estimated DON concentrations above the limit of tolerance. Human and animal exposure to mycotoxins in the Netherlands appears to be small but chronic. The information presented in this paper illustrates the need for an annual evaluation of the crop for fusarium head blight incidence and mycotoxin content, and the necessity of fusarium head blight resistant wheat cultivars.
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    URL: http://dx.doi.org/10.1007/BF01974256
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    Polymorphism of prolamins and variability of grain quality (1982)
    Springer
    Plant foods for human nutrition 31 (1982), S. 243-249 
    Details
    ISSN: 1573-9104
    Keywords: wheat ; grain protein ; prolamin ; inheritance ; chromosome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Electrophoretic gliadin components are inherited as highly stable blocks of linked components. Multiple allelism of blocks controlled by polygenic loci of six gliadin-coding chromosomes has been revealed. The allelic states of these loci are interrelated with the variability of grain. The regularities discovered permit the use of gliadin polymorphism for estimation of genotype properties in breeding programs.
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    URL: http://dx.doi.org/10.1007/BF01108633
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    The genetics of gliadin and glutenin, the major storage proteins of the wheat endosperm (1982)
    Springer
    Plant foods for human nutrition 31 (1982), S. 229-241 
    Details
    ISSN: 1573-9104
    Keywords: wheat ; genetics ; endosperm ; seed proteins ; gliadins ; glutenins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper reviews our present knowledge of the chromosomal location of the genes that control the synthesis of gliadins and glutenins, the two major groups of storage protein in the endosperm of wheat (Triticum aestivum). Allelic relationships and genetic linkage between genes are also discussed. The areas that require futher investigation are identified.
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    URL: http://dx.doi.org/10.1007/BF01108632
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    Effect of cereal-legumechapati diets on absorption and retention of calcium (1992)
    Springer
    Plant foods for human nutrition 42 (1992), S. 165-173 
    Details
    ISSN: 1573-9104
    Keywords: Calcium ; absorption ; wheat ; Bengal gram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheatchapati, wheat+Bengal gram (80∶20 and 70∶30)chapatis and casein diets, at 10 percent protein level, were fed to rats for 12 weeks to study the effect on calcium utilization. The supplementation of Bengal gram to wheat diets significantly improved the calcium absorption. The urinary calcium excretion in wheat+Bengal gramchapati diets was significantly less than that of wheatchapati diet fed group. The in vivo45Ca absorption (CPM/100 μl serum) was also less in wheatchapati group as compared to wheat+Bengal gramchapati diets fed groups. The supplementation of legumes to cereals improved the calcium utilization and it may be concluded that there will be no risk of occurance of protein induced hypercalciuria.
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    URL: http://dx.doi.org/10.1007/BF02196469
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    Improvement of bread wheat gluten quality (1983)
    Springer
    Plant foods for human nutrition 33 (1983), S. 121-126 
    Details
    ISSN: 1573-9104
    Keywords: wheat ; gliadin ; glutenin subunits ; correlation ; gluten quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01091297
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    A small scale device for wheat gluten separation (1983)
    Springer
    Plant foods for human nutrition 33 (1983), S. 161-168 
    Details
    ISSN: 1573-9104
    Keywords: wheat ; gluten ; small scale extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A new small scale device for wheat gluten separation is described and the results of the first experiments on the influence of extraction conditions on different flours are reported.
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    URL: http://dx.doi.org/10.1007/BF01091303
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    Nutritive value of protein fractions extracted from soybean, rapeseed and wheat flours in the rat (1984)
    Springer
    Plant foods for human nutrition 34 (1984), S. 243-251 
    Details
    ISSN: 1573-9104
    Keywords: nutritive value ; protein fractions ; rapeseed ; soybean ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The nutritive value of various protein fractions was studied. Fractions 2S and 12S from rapeseed, 2S, 7S and 11S from soybean were obtained by dissolution in ammonium sulfate solutions. Albumin-globulin, gluten, glutenin and gliadin fractions from wheat were obtained by dissolution in salted water (albumin-globulin), acetic acid (glutenin) and alcohol (gliadin). Liveweight gains, protein efficiency ratio (PER) and apparent digestibility coefficient (ADC) were used as measures of the nutritive value. The protein fractions had a lower nutritive value than the unfractionated proteins except for the albumin-globulin fraction of wheat which had a nutritive value higher than that of the unfractionated wheat protein. PER obtained with the rapeseed 2S and 12S fractions were 2.49 and 2.21, respectively, as compared to 2.64 for unfractionated rapeseed. With soybean fractions, PER were 0.92 for 2S, — 0.007 for 7S and 1.47 for 11S, as compared to 2.19 for the original protein. The wheat albumin-globulin fraction gave a PER of 2.78, as compared to 1.45 for the unfractionated wheat protein. Gluten, glutenin and gliadin fractions had a lower PER than that of unfractionated wheat protein. ADC of all fractions were higher than those of the original proteins. The difference in liveweight gains and PER observed between protein fractions can be partially explained on the basis of the essential amino acid content.
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    Patterns of trait variation in the diploid wheats (Triticum, Aegilops) and the tetraploid speciesTriticum diccocoides (1983)
    Springer
    Plant systematics and evolution 143 (1983), S. 257-275 
    Details
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Aegilops ; Triticum ; T. diccocoides ; wheat ; Adaptation ; trait variation ; factor analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eighteen earliness and morphological traits were examined in from 2 to 14 lines each of 10 diploid members of the wheat complex,Triticum-Aegilops, and 15 lines of the tetraploid speciesTriticum diccocoides. In general, earliness traits have the greatest relative between line vs. within line variation of all the traits examined. Within species, earliness traits are the principle set of characters around which evolve between line trait differences, one of the most important oich is leaf dimensions. At the genus level, earliness traits are independent of leaf dimensions and plant height characters. Thus, the pattern of evolution at the genus level is different from that exhibited in each of the species. Biological and evolutionary implications of these findings are discussed.
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    Analysis of programmed cell death in wheat endosperm reveals differences in endosperm development between cereals (1999)
    Springer
    Plant molecular biology 39 (1999), S. 915-926 
    Details
    ISSN: 1573-5028
    Keywords: programmed cell death ; wheat ; endosperm ; ethylene ; nucleases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Although maize endosperm undergoes programmed cell death during its development, it is not known whether this developmental feature is common to cereals or whether it arose inadvertently from the selection process that resulted in the enlarged endosperm of modern maize. Examination of wheat endosperm during its development revealed that this tissue undergoes a programmed cell death that shares features with the maize program but differs in some aspects of its execution. Cell death initiated and progressed stochastically in wheat endosperm in contrast to maize where cell death initiates within the upper central endosperm and expands outward. After a peak of ethylene production during early development, wheat endosperm DNA underwent internucleosomal fragmentation that was detectable from mid to late development. The developmental onset and progression of DNA degradation was regulated by the level of ethylene production and perception. These observations suggest that programmed cell death of the endosperm and regulation of this program by ethylene is not unique to maize but that differences in the execution of the program appear to exist among cereals.
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    URL: http://dx.doi.org/10.1023/A:1006134027834
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    Control of Mn status and infection rate by genotype of both host and pathogen in the wheat take-all interaction (1990)
    Springer
    Plant and soil 123 (1990), S. 267-275 
    Details
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; genotypes ; interaction ; manganese ; oxidation ; take-all ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Take-all is a world-wide root-rotting disease of cereals. The causal organism of take-all of wheat is the soil-borne fungus Gaeumannomyces graminis var tritici (Ggt). No resistance to take-all, worthy of inclusion in a plant breeding programme, has been discovered in wheat but the severity of take-all is increased in host plants whose tissues are deficient for manganese (Mn). Take-all of wheat will be decreased by all techniques which lift Mn concentrations in shoots and roots of Mn-deficient hosts to adequate levels. Wheat seedlings were grown in a Mn-deficient calcareous sand in small pots and inoculated with four field isolates of Ggt. Infection by three virulent isolates was increased under conditions which were Mn deficient for the wheat host but infection by a weakly virulent isolate, already low, was further decreased. Only the three virulent isolates caused visible oxidation of Mn in vitro. The sensitivity of Ggt isolates to manganous ions in vitro did not explain the extent of infection they caused on wheat hosts. In a similar experiment four Australian wheat genotypes were grown in the same Mn-deficient calcareous sand and inoculated with one virulent isolate of Ggt. Two genotypes were inefficient at taking up manganese and were very susceptible to take-all, one was very efficient at taking up manganese and was resistant to take-all, and the fourth genotype was intermediate for both characters. All genotypes were equally resistant under Mn-adequate conditions.
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    Nucleotide sequences of the genes for the alpha, beta and epsilon subunits of wheat chloroplast ATP synthase (1985)
    Springer
    Plant molecular biology 4 (1985), S. 333-345 
    Details
    ISSN: 1573-5028
    Keywords: wheat ; chloroplast genes ; ATP synthase ; methionine tRNA gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequences of the chloroplast genes for the alpha, beta and epsilon subunits of wheat chloroplast ATP synthase have been determined. Open reading frames of 1512 bp, 1494 bp and 411 bp are deduced to code for polypeptides of molecular weights 55201, 53796 and 15200, identified as the alpha, beta and epsilon subunits respectively by homology with the subunits from other sources and by amino acid sequencing of the epsilon subunit. The genes for the beta and epsilon subunits overlap by 4 bp. The gene for methionine tRNA is located 118 bp downstream from the epsilon subunit gene. Comparisons of the deduced amino acid sequences of the alpha and beta subunits with those from other species suggest regions of the proteins involved in adenine nucleotide binding.
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    URL: http://dx.doi.org/10.1007/BF02418255
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    Role of bacterial cyanide production in differential reaction of plant cultivars to deleterious rhizosphere pseudomonads (1991)
    Springer
    Plant and soil 133 (1991), S. 93-100 
    Details
    ISSN: 1573-5036
    Keywords: cultivars ; growth inhibition ; hydrogen cyanide ; lettuce ; Pseudomonas fluorescens ; volatile metabolites ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lettuce and wheat cultivars, differing in reaction to root inoculation with plant growth-inhibitory bacteria, were tested for sensitivity to (i) gaseous metabolites produced by deleterious, cyanogenic isolates of Pseudomonas fluorescens, and to (ii) pure cyanide. Reactions were read as shoot and/or root elongation after exposure of seedlings to the volatiles in vitro. Lettuce cultivar Salad Bowl was significantly less sensitive than cv. Montana, both to bacterial volatiles and to pure cyanide, and a similar difference between these cultivars was also obtained in greenhouse experiments where bacteria were inoculated directly on the roots. Cultivar differences were, however, not recorded, either in vitro or in the greenhouse, when the bacteria were grown on a medium which did not support cyanide production. In wheat, a difference in sensitivity to bacteria-produced volatiles was recorded between two cultivars (Drabant and Besso) differing in reaction to bacterial inoculation, but in contrast to lettuce cultivars, the wheat cultivars tested did not react differentially to pure cyanide. The results suggest that in lettuce differential sensitivity to cyanide is one factor behind cultivar differences in reaction to the bacteria tested, even though bacterial metabolites other than cyanide may contribute to the plant growth inhibition recorded. In wheat, however, differential cultivar responses to these bacteria could not be shown to be related to cyanide.
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    Effects of rainfall on the use of foliar analysis for diagnosing boron toxicity in field-grown wheat (1992)
    Springer
    Plant and soil 140 (1992), S. 311-314 
    Details
    ISSN: 1573-5036
    Keywords: boron toxicity ; diagnosis ; foliar analysis ; mineral nutrition ; nutrient leaching ; rain ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of rain on foliage elemental composition, especially B, was assessed using samples of wheat collected at three harvests from a field trial conducted in soil containing excessive levels of B. Moderate rainfall substantially decreased both the B concentration and content of whole shoots and young leaves. The change in B concentration due to rain suggests that foliar analysis is unreliable for diagnosing B toxicity. For the other elements examined (Ca, Cu, Mg, Mn, Mg, P, S, Zn), rainfall had little effect.
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    The root activity of cereals and peas when grown in pure stands and mixtures (1992)
    Springer
    Plant and soil 142 (1992), S. 281-285 
    Details
    ISSN: 1573-5036
    Keywords: barley ; mixed-cropping ; peas ; root activity ; rooting-depth ; tracers ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Lithium was used as a non-radioactive tracer to investigate the root activity of two cereals (wheat and barley), and of two contrasting cultivars of pea (leafy and semi-leafless), both in pure stands and in mixtures. The mixtures included combinations of each cereal with each pea cultivar in single rows, alternative rows and cross-drilled. Total lithium uptake (mg m-2) was higher for wheat than for barley, and higher for semi-leafless pea than for leafy peas. Growing cereals with peas reduced the total lithium uptake by peas, compared with pure stands, especially in alternate-row mixtures. Growing peas with cereals only reduced the total Li uptake by cereals when they were cross-drilled. The Li uptake by wheat, barley and peas generally decreased with soil depth in a similar manner; however, semi-leafless peas absorbed proportionately more Li from close to the soil surface than did leafy peas. Both pea cultivars absorbed more Li at 10–20 cm depth when grown in intimate mixtures with cereals, compared with less intimate mixtures or pure stands. The potential of lithium as a non-radioactive tracer in mixed-cropping studies is briefly discussed.
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    Comparative species responses to boron on a Typic Tropaqualf in Northern Thailand (1988)
    Springer
    Plant and soil 106 (1988), S. 15-21 
    Details
    ISSN: 1573-5036
    Keywords: black gram ; boron deficiency ; borax rates ; green gram ; hollow heart ; hot-water-soluble boron ; kernel boron ; leaf boron ; peanut ; rice ; soybean ; sunflower ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of boron (B) on peanut and soybean was examined in two omission and one B fertilizer rate trial on a Typic Tropaqualf in Northern Thailand. The B rate trial was combined with a comparison of the response of sunflower, green gram, black gram, wheat, and rice in addition to peanut and soybean grown in irrigated rice-based cropping sequences over two years. Omitting B induced the hollow heart symptom in 10% of peanut kernels with the incidence of hollow hearts closely related to B concentration in the kernels. Omission of B had no effect on the appearance of soybean seed or on the grain yield of either soybean or peanut. In the B rate experiment, omitting B depressed grain yield by 50% in sunflower and by 40% to 80% in black gram, induced B deficiency symptoms in green gram and the hollow heart symptom in peanut kernels, but had not significant effect on the grain yield of soybean, peanuts, rice, or wheat. B deficiency apparently depressed grain yield in black and green gram by delaying or inhibiting reproductive development thus reducing pod set.
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    Empirical models to approximate calcium and magnesium ameliorative effects and genetic differences in aluminium tolerance in wheat (1992)
    Springer
    Plant and soil 144 (1992), S. 281-287 
    Details
    ISSN: 1573-5036
    Keywords: aluminium ; aluminium tolerance ; calcium ; magnesium ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The activities of inorganic, monomeric aluminium (Al) species in the root environment are important in the toxicity of Al to plant roots, which may be ameliorated by increased activities of basic cations. Additionally, it has been suggested that electro-chemical processes in walls of root cells play a role in Al tolerance. Empirical models were proposed to accomodate genetic and calcium (Ca) and magnesium (Mg) ameliorative effects on Al toxicity. The models were tested using data from a solution culture study (with ionic strength 1.6 to 8.6 mM) in which wheat (Triticum aestivum L.) cvv. Warigal (Al-sensitive) and Waalt (Al-tolerant) were grown for 28 d at 0, 10 and 20 μM Al, in factorial combination with 200, 400, 800 and 1600 μM Ca and 100, 200, 400 and 800 μM Mg. There was a poor relationship between relative total dry mass (TDM) (calculated as a percentage of the average TDM of each cultivar in the absence of added Al) and the activity of Al3+ or the sum of the activities of the monomeric Al species in solution. A model based on the ratios of activities of cations in solution, taking valency into consideration, was more successful, accounting for ca 85% of the observed variation in relative TDM. There were no systematic variations between observed values and those estimated by the model.
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    Extractable sulphate and organic sulphur in soils and their availability to plants (1994)
    Springer
    Plant and soil 164 (1994), S. 243-250 
    Details
    ISSN: 1573-5036
    Keywords: organic sulphur ; soil testing ; sulphate ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Ten soils collected from the major arable areas in Britain were used to assess the availability of soil sulphur (S) to spring wheat in a pot experiment. Soils were extracted with various reagents and the extractable inorganic SO4-S and total soluble S(SO4-S plus a fraction of organic S) were determined using ion chromatography (IC) or inductively-coupled plasma atomic emission spectrometry (ICP-AES), respectively. Water, 0.016 M KH2PO4, 0.01 M CaCl2 and 0.01 M Ca(H2PO4)2 extracted similar amounts of SO4-S, as measured by IC, which were consistently smaller than the total extractable S as measured by ICP-AES. The amounts of organic S extracted varied widely between different extractants, with 0.5 M NaHCO3 (pH 8.5) giving the largest amounts and 0.01 M CaCl2 the least. Organic S accounted for approximately 30–60% of total S extracted with 0.016 M KH2PO4 and the organic C:S ratios in this extract varied typically between 50 and 70. The concentrations of this S fraction decreased in all soils without added S after two months growth of spring wheat, indicating a release of organic S through mineralisation. All methods tested except 0.5 M NaHCO3-ICP-AES produced satisfactory results in the regression with plant dry matter response and S uptake in the pot experiment. In general, 0.016 M KH2PO4 appeared to be the best extractant and this extraction followed by ICP-AES determination was considered to be a good method to standardise on.
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    URL: http://dx.doi.org/10.1007/BF00010076
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    Soil conduciveness to take-all of wheat: Influence of the nitrogen fertilizers on the structure of populations of fluorescent pseudomonads (1992)
    Springer
    Plant and soil 145 (1992), S. 29-36 
    Details
    ISSN: 1573-5036
    Keywords: ammonium ; cyanides ; Gaeumannomyces graminis var tritici ; nitrate ; nitrogen fertilizer ; pseudomonads ; soil conduciveness ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a field cropped with wheat, a high and low level of soil conduciveness to take-all were induced by applying a nitrogen fertilizer with either calcium nitrate or ammonium sulphate. From these two soils, two representative populations of fluorescent pseudomonads were tested for their in situ behaviour. Take-all index and root dry weight were assessed on plants cropped in soils infested with Gaeumannomyces graminis var tritici (Ggt) and each bacterized with one of the isolates of fluorescent pseudomonads. The bacteria tested can be split into three groups: antagonists which reduce take-all, deleterious isolates which aggravate the disease and neutral without evident effect on the disease. The predominance of antagonistic fluorescent pseudomonads in the NH4-treated soil and the predominance of deleterious ones in the NO3-treated soil was confirmed after statistical analysis. The microbial impact on take-all must be more considered as the resulting effect of divergent activities of both rhizobacteria types than the only consequences of the presence of antagonistic pseudomonads. All the high cyanogenic pseudomonads were antagonists in situ and were more numerous in the NH4-treated soil than in the NO3-treated soil.
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    URL: http://dx.doi.org/10.1007/BF00009538
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    Relationships between take-all, soil conduciveness to the disease, populations of fluorescent pseudomonads and nitrogen fertilizers (1992)
    Springer
    Plant and soil 145 (1992), S. 17-27 
    Details
    ISSN: 1573-5036
    Keywords: ammonium ; Gaeumannomyces graminis var tritici ; nitrate ; nitrogen fertilizers ; fluorescent pseudomonads ; root system ; soil conduciveness ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Take-all of wheat, caused by Gaeumannomyces graminis var tritici (Ggt), is reduced by ammoniacal fertilizers as compared to nitrate sources. This influence of nitrogen on the disease is only observed on nodal roots at flowering. But soil conduciveness to take-all, as measured in a soil bioassay, is modified earlier. Forty days after nitrogen application at early tillering, the NH4-treated soil became less conducive than the NO3-treated one. When nitrogen applications are done at sowing and at tillering, differences in disease propagation between the two soils are enhanced. Results from four years of experimentation show that when the level of natural soil inoculum is high, disease severity is reduced by ammonium, showing an effect on the parasitic phase of Ggt. At a low level of natural inoculum the effect of the source of nitrogen is mainly observed on the percent of infected plants, indicating that the saprophytic and preparasitic phases are affected. Rhizospheric bacterial populations increase from sowing to tillering, but differences on take-all conduciveness after tillering are not correlated with differences in the amounts of aerobic bacteria or fluorescent pseudomonads isolated from soils treated with different sources of nitrogen. Qualitative changes in fluorescent Pseudomonas spp. populations, like in vitro antagonism, are more likely to explain differences in soil conduciveness to take-all than are quantitative changes in this group. Nevertheless, the introduction of Ggt in a cropped soil leads to a greater increase in fluorescent pseudomonads populations than in total aerobic bacteria. The delay between reducing soil conduciveness and reducing disease in the field with ammonium nitrogen fertilization, the qualitative change of fluorescent pseudomonads populations and the role of necroses in rhizobacteria multiplication, provide information leading to our representation of a dynamic model based on the differentiation of the wheat root system into seminal and nodal roots.
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    URL: http://dx.doi.org/10.1007/BF00009537
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    Effect of various metal ions on growth of two wheat lines known to differ in aluminium tolerance (1993)
    Springer
    Plant and soil 155-156 (1993), S. 489-492 
    Details
    ISSN: 1573-5036
    Keywords: aluminium ; analog ; boron ; copper ; gallium ; iron ; lanthanum ; manganese ; scandium ; tolerance ; Triticum aestivum ; toxicity ; wheat ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of aluminium (Al), manganese (Mn), zinc (Zn), copper (Cu), boron (B), iron (Fe), gallium (Ga), scandium (Sc) and lanthanum (La) on growth of an Al-tolerant and an Al-sensitive line of wheat (Triticum aestivum L.) were measured in solution culture. The concentrations of nutrients in the basal nutrient solution were (μM) 500 Ca, 100 Mg, 300 K, 600 N (150 NH4, 450 NO3), 600 SO4, 2.5 P, 3 B, 2.5 Fe, 0.5 Zn, 0.5 Mn, 0.1 Cu at a pH of 4.7. The major solution nutrient concentrations were maintained at the nominal concentration with monitoring, frequent additions and weekly renewal. Differentiation in yield between the Al-tolerant and Al-sensitive line only occurred in the presence of Al indicating that, in the long term, none of the other metals tested could be used as an analog for Al. The visual symptoms in the roots of Cu toxicity (in both lines) and Al toxicity (in the sensitive line) were similar. The solution concentration (μM) at which yield of the roots of the tolerant line was reduced by 50% was, in order of increasing tolerance, Cu 0.5, Sc 1.1, La 7.1, Ga 8.6, Al 15, Zn 19, Fe 84, B 490 and Mn 600.
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    URL: http://dx.doi.org/10.1007/BF00025090
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    What is the significance of herbicide-nutrient interactions on wheat? (1993)
    Springer
    Plant and soil 155-156 (1993), S. 529-532 
    Details
    ISSN: 1573-5036
    Keywords: chlorsulfuron ; mineral nutrition ; sulfonylurea ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sulfonylurea herbicides have been found to decrease the uptake and utilization of some nutrients by wheat. This paper reviews the effects of sulfonylureas on nutrient uptake, proposes physiological mechanisms which might explain the effects; and examines the agronomic implications.
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    URL: http://dx.doi.org/10.1007/BF00025100
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    Modelling nutrient responses in the field (1993)
    Springer
    Plant and soil 155-156 (1993), S. 57-66 
    Details
    ISSN: 1573-5036
    Keywords: nitrogen ; wheat ; simulation ; yield-response curve
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Models of the yield responses of crops to applied nutrients are a recent addition to the methods available for making fertilizer recommendations. They have a place in integrating nutrient information with information on other factors which affect yield and its response to added nutrients. This review deals with nitrogen models classified into three groups: those which predict yield-response curves based on empirical factors; those which simulate the yield response from complex simulation models of many processes regulating crop growth and the soil environment; and those which aim to simulate yield and selected processes based on simplified functional relationships which apply to a target region or industry. Three case studies representing the three classes of model are drawn from research on dryland wheat in different parts of Australia. They show examples in which models provide information which is unobtainable from experimental procedures and which provide information useful to farmers in making decisions about fertilizers. Suggestions are made for future developments in crop-nutrient modelling including further comparisons of models, linkage of models with tissue tests, modelling co-limiting nutrients, deciding on the appropriate level of detail within a model and the need for methods for calibrating and testing models on attributes other than yield alone.
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    URL: http://dx.doi.org/10.1007/BF00024984
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    Modifications of the carbon and nitrogen allocations in the plant (Triticum aestivum L.) soil system in response to increased atmospheric CO2 concentration (1993)
    Springer
    Plant and soil 157 (1993), S. 215-225 
    Details
    ISSN: 1573-5036
    Keywords: carbon partitioning ; CO2 enrichment ; nitrogen mineralization ; nitrogen partitioning ; rhizosphere ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The aim of this work was to examine the response of wheat plants to a doubling of the atmospheric CO2 concentration on: (1) carbon and nitrogen partitioning in the plant; (2) carbon release by the roots; and (3) the subsequent N uptake by the plants. The experiment was performed in controlled laboratory conditions by exposing fast-growing spring wheat plants, during 28 days, to a 14CO2 concentration of 350 or 700 μL L−1 at two levels of soil nitrogen fertilization. Doubling CO2 availability increased total plant production by 34% for both N treatment. In the N-fertilized soil, the CO2 enrichment resulted in an increase in dry mass production of 41% in the shoots and 23% in the roots; without N fertilization this figure was 33% and 37%, respectively. In the N-fertilized soil, the CO2 increase enhanced the total N uptake by 14% and lowered the N concentration in the shoots by 23%. The N concentration in the roots was unchanged. In the N-fertilized soil, doubling CO2 availability increased N uptake by 32% but did not change the N concentrations, in either shoots or roots. The CO2 enrichment increased total root-derived carbon by 12% with N fertilization, and by 24% without N fertilization. Between 85 and 90% of the total root derived-14C came from respiration, leaving only 10 to 15% in the soil as organic 14C. However, when total root-derived 14C was expressed as a function of root dry weight, these differences were only slightly significant. Thus, it appears that the enhanced carbon release from the living roots in response to increased atmospheric CO2, is not due to a modification of the activity of the roots, but is a result of the increased size of the root system. The increase of root dry mass also resulted in a stimulation of the soil N mineralization related to the doubling atmospheric CO2 concentration. The discussion is focused on the interactions between the carbon and nitrogen allocation, especially to the root system, and the implications for the acquisition of nutrients by plants in response to CO2 increase.
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    URL: http://dx.doi.org/10.1007/BF00011050
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    A review of phosphorus efficiency in wheat (1992)
    Springer
    Plant and soil 146 (1992), S. 163-168 
    Details
    ISSN: 1573-5036
    Keywords: genetic variability ; NIR ; ploidy ; phosphorus ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract More efficient utilization of phosphorus by wheat plants is needed to extend the useful life of the phosphate reserves in the world, to reduce the cost of producing crops, and to improve the value of the grain and the straw produced. In this paper definitions of efficient use of phosphorus by wheat are reviewed, genotypic variation in phosphorus efficiency is reported, some consequences of breeding for greater efficiency are discussed, and ways to select more efficient genotypes are suggested.
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    URL: http://dx.doi.org/10.1007/BF00012009
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    Biofumigation: Isothiocyanates released frombrassica roots inhibit growth of the take-all fungus (1994)
    Springer
    Plant and soil 162 (1994), S. 107-112 
    Details
    ISSN: 1573-5036
    Keywords: canola ; glucosinolates ; Indian mustard ; take-all ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The presence of root tissue of the brassicas canola and Indian mustard inhibited growth of pure cultures of the fungal pathogen which causes take-all of wheat [Gaeumannomyces graminis (Sacc.) Arx and Oliver var.tritici, abbreviated as Ggt]. Ggt growth was generally inhibited more in the presence of Indian mustard roots than canola roots. Dried irradiated roots were consistently effective in reducing Ggt growth, but growth inhibition by young live roots and macerated roots was not consistent. The inhibitory compound(s) were shown to be volatile because the symmetry of Ggt growth was not affected by the proximity of theBrassica tissue. Volatile breakdown products from maceratedBrassica roots were identified using a gas chromatograph-mass spectrometer. The major compounds found were isothiocyanates (ITCs). Canola roots released mostly methyl ITC and Indian mustard roots released mostly phenylethyl ITC. Low concentrations of these and related compounds inhibited growth of Ggt in pure culture when supplied as the vapour of pure chemicals in concentrations within the range expected during breakdown ofBrassica roots in soil.
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    URL: http://dx.doi.org/10.1007/BF01416095
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    3Hthymidine incorporation of rhizosphere bacteria influenced by plant N-status (1994)
    Springer
    Plant and soil 162 (1994), S. 113-116 
    Details
    ISSN: 1573-5036
    Keywords: ammonium ; nitrate ; split-roots ; root exudate ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of plant-root N-status on bacterial growth in the rhizosphere was studied with 5-week-old wheat plants grown in soil with low N content obtained by mixing 9:1 gravel:sandy loam. As a consequence of N limitation, significant increase in3Hthymidine (Tdr) incorporation rate occured 3 days after addition of 30 mM ammonium compared to controls without ammonium. Plants were grown with split-roots to separate the effect of soil N from effect of plant root derived organic matter-N on bacterial activity. The increase in nitrate concentration from 10 mM to 30 mM at one part of the root system led to significant increased3HT dr incorporation in the rhizosphere at the other part of root system after 4 days showing that the composition of root exudates became more favourable for bacterial growth when plants were fertilized with the higher level of nitrate.
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    URL: http://dx.doi.org/10.1007/BF01416096
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    Growth, biomass allocation and water use efficiency of two wheat cultivars in a mediterranean environment; a pot experiment under field conditions (1994)
    Springer
    Plant and soil 162 (1994), S. 241-247 
    Details
    ISSN: 1573-5036
    Keywords: biomass allocation ; carbon isotope discrimination ; growth ; water use efficiency ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plants of two bread wheat cultivars,Triticum aestivum L. ‘Katya Al’ and ‘Mexipak 65’, were grown in pots during the crop season in the field in NW Syria, a region with a Mediterranean climate. The experiment involved two treatments. Control plants were well-watered throughout the experiment (watering to 0.22 g water g−1 dry soil). In the second treatment, water was withheld from the plants until soil water content had decreased to 0.10 g water g−1 dry soil, the level that was maintained thereafter. Water use was measured by weighing the pots, and growth by destructive sampling. Growth of Katya and Mexipak was similar. Mexipak had a lower (though not significant atp〈0.05) plant water use efficiency (WUEp) in both treatments due to higher rates of water loss. On a leaf area basis differences in water use were especially high since Mexipak had a smaller total leaf area. In spite of a smaller investment in photosynthesizing area, Mexipak achieved similar growth as Katya. Carbon isotope discrimination and organic nitrogen concentration (both higher for Mexipak) suggest that Mexipak accomplished higher mean net photosynthetic rates with a higher mean leaf diffusive conductance, higher intercellular carbon dioxide partial pressure, and possibly a greater investment in the photosynthetic apparatus compared to Katya. Differences in carbon isotope discrimination suggest a larger difference in average photosynthetic WUE (net photosynthesis/transpiration) than in plant WUE. This could indicate that loss of carbon in respiration was greater in Katya. Gas exchange measurements on the youngest fully expanded leaves showed only minor differences between the cultivars. It is hypothesized that Mexipak, with a smaller total leaf area, is able to maintain high leaf conductance and photosynthesis for a longer period of time during the day or during the life span of leaves.
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    URL: http://dx.doi.org/10.1007/BF01347711
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    Genotype-environment interaction and correlation between vegetative and grain production measures of potassium use-efficiency in wheat (T. aestivum L.) grown under potassium stress (1993)
    Springer
    Plant and soil 151 (1993), S. 39-44 
    Details
    ISSN: 1573-5036
    Keywords: efficiency ratio ; harvest index ; potassium ; Triticum aestivum ; utilization index ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nutrient use-efficiency (NUE) is commonly measured in relation to vegetative growth without regard for economic productivity of crops whose valued product is reproductive. Although vegetative measures can be useful, particularly in forage crops, their validity in the quantification of NUE in grain crops is questionable. This study was undertaken primarily to examine the relationship between vegetative and economic potassium use-efficiency (KUE) in several wheat (T. aestivum) genotypes under conditions of potassium stress. Genotype environment interaction for vegetative KUE was also examined. Vegetative KUE was assessed as shoot fresh weight, efficiency ratio (VKER) and utilization index (VKUI) whereas economic KUE was evaluated as grain weight, total weight and economic efficiency ratio (EKER). Significant genotype-environment interactions for shoot weight, VKER and VKUI were observed. In some instances interaction was associated with crossovers between genotypes indicating that it can affect selection. Correlations between vegetative and economic measures of KUE were generally nonsignificant, but negative and significant for shoot weight of three-week-old plants and grain weight. It appears that if genotypes differ considerably for harvest index and it is not positively correlated with total biomass, vegetative measures of KUE are unreliable as indicators of economic KUE. Therefore, economic rather than vegetative measures should be used to evaluate KUE in crops whose valued product is reproductive. ei]{gnH}{fnMarschner} ei]{gnH.}{fnLambers}
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    URL: http://dx.doi.org/10.1007/BF00010784
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    The influence of crop management on the water balance of lupin and wheat crops on a layered soil in a Mediterranean climate (2000)
    Springer
    Plant and soil 221 (2000), S. 239-251 
    Details
    ISSN: 1573-5036
    Keywords: drainage ; duplex soil ; evapotranspiration ; lupin ; waterlogging ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The influence of time of sowing and sowing density on evapotranspiration and drainage loss beneath wheat (Triticum aestivum cv. Spear) and lupin (Lupinus angustifolius cv. Gungurru) crops grown on a layered soil was investigated for three seasons in a Mediterranean climate in Western Australia. The aim of the study was to investigate whether managing crops to maximise their canopy growth would increase their water use and minimise groundwater recharge contributing to dryland salinity. A soil water balance approach was used to estimate evapotranspiration, with changes in soil water content measured with a neutron water meter. The study was carried out on a layered soil typical of agricultural soils in the region with variable depth to clay (0.22–0.38 m) and a marked contrast in hydraulic properties between the topsoil and subsoil. As a result of the low permeability subsoil, a perched water table occurred in the sandy topsoil in each of the three seasons under study during winter when rainfall was high and potential evaporation low. Perched water tables persisted for 2–3 months, with hydraulic gradients consistently downward causing drainage losses to occur. Although crop management had a large influence on shoot and root development, evapotranspiration from the different treatments was generally similar. Drainage losses were not influenced by either crop type, time of sowing or sowing density, because potential evaporation and hence evapotranspiration was low during the period when drainage losses occurred. The total drainage loss measured in each season was different, with losses ranging from 20.1 to 22.2 mm in 1990, from 40.4 to 46.7 mm in 1991 and from 49.4 to 66.6 mm in 1992. The increase in drainage loss from 1990 to 1992 was a result of progressively more seasonal rainfall in 1990, 1991 and 1992. It was concluded that there was little scope to increase water use and decrease deep drainage through crop management for sites with climatic conditions where winter rainfall exceeds potential evaporation.
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    URL: http://dx.doi.org/10.1023/A:1004732706073
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    Non-structural carbohydrate: Analysis by near infrared reflectance spectroscopy and its importance as an indicator of plant growth (1993)
    Springer
    Plant and soil 155-156 (1993), S. 243-246 
    Details
    ISSN: 1573-5036
    Keywords: fructans ; NIR ; nitrogen ; non-structural carbohydrates ; rice ; starch ; stress ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant shoot samples are frequently analysed to assess if crops require additional nitrogen or mineral elements to maintain satisfactory growth. If plant growth is limited by temperature, water stress, disease, lodging or a mineral deficiency, non-structural carbohydrates (NSC) may be accumulated in, or depleted from, tissues especially those in the lower stems. Plant testing laboratories do not routinely analyse NSC to assist in the identification of plant stress probably because skilled technicians and time are required for the wet chemical determination. In this paper we report that routine determination of NSC is possible using near-infrared reflectance spectroscopy; the errors of determination are comparable with traditional chemical methods. The concentration of NSC in the shoots of rice grown in south eastern Australia ranges from 1.6 to 22.8%, as starch. In the shoots of wheat grown in eastern Australia the range is from 2.4 to 35.2%, as fructans. In both crops the NSC content is highly inversely correlated with the shoot nitrogen content. Based on data from commercial wheat and rice crops we suggest that the ratio between nitrogen and NSC can be used to identify crops in which growth has been limited by a stress other than nitrogen and so are unlikely to show the predicted response to an application of nitrogen fertilizer.
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    URL: http://dx.doi.org/10.1007/BF00025029
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    A new approach to quantify the utilization of non-exchangeable soil potassium by plants (1993)
    Springer
    Plant and soil 149 (1993), S. 235-243 
    Details
    ISSN: 1573-5036
    Keywords: De ; exchangeable K ; K diffusion ; K release ; K utilization ; model calculation ; non-exchangeable K ; root hairs ; sugar beet ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To predict the contribution of soil K fractions of different mobility to K supply of plants, the kinetics of K release from soil was related to the kinetics of K uptake of young sugar beet and wheat plants. For this purpose K release rates from soil were measured by continuously percolating samples of a luvisol with 0.01 M CaCl2 solution and effective diffusion coefficients, De, were determined. Two soil K fractions of different mobility were obtained. De values of the more mobile ‘exchangeable K’ and the less mobile ‘non-exchangeable’ K fraction were found to be 58.9 × 10−9 and 8.2 × 10−9 cm2 s−1, respectively. In a pot experiment, sugar beet and wheat plants were grown, for 15 days and both root growth and K uptake were measured. K uptake kinetics of both crops was determined in a separate experiment using flowing solution culture. To integrate these data quantitatively, the simulation model of Claassen et al. (1986) was applied. Results show that calculated total K uptake agreed closely with real K uptake of the plants. On this basis, 64 and 79% of the K taken up by wheat and sugar beet plants was derived from the rapidly released ‘exchangeable’ and 21–36% from the less mobile ‘non-exchangeable’ soil K fraction.
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    URL: http://dx.doi.org/10.1007/BF00016614
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    The 2,4-D-induced wheat para-nodules are modified lateral roots with structure enhanced by rhizobial inoculation (1994)
    Springer
    Plant and soil 159 (1994), S. 121-129 
    Details
    ISSN: 1573-5036
    Keywords: 2,4-dichlorophenoxyacetate ; para-nodule ; Rhizobium trifolii ; structure ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nodular outgrowths (para-nodules or p-nodules) on the roots of wheat (Triticum aestivum L.) cv. Miskle seedlings were induced by treatment with 0.3 and 0.6mg L−1 2,4-dichlorophenoxyacetate (2,4-D). When co-inoculated with Rhizobium trifolii strain ATCC 14480, more p-nodules were formed at these levels and p-nodulation occured at 0.1 mg L−1 indicating that inoculation enhances 2,4-D-induced p-nodulation. Similar to lateral roots, the p-nodules arose from the pericycle opposite the phloem tissues and were free from the cortical cells of the parental root at all stages of development. Structurally, the p-nodules exhibited tissue differentiation. They possessed a highly organized central vascular cylinder connected to that of the parent root, an endodermis, a cap, and an apical and lateral meristems. P-nodules formed by 2,4-D treatment alone were irregularly lobed due to uncoordinated activity of the apical meristem, while those in the combined 2,4-D and inoculation treatment were more globose. The results of the present study indicate that the 2,4-D-induced wheat p-nodules are modified lateral roots, the structure of which is enhanced by rhizobial inoculation.
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    URL: http://dx.doi.org/10.1007/BF00009294
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    Regulation of NH4 + uptake in wheat plants: Effect of root ammonium concentration and amino acids (1993)
    Springer
    Plant and soil 151 (1993), S. 211-218 
    Details
    ISSN: 1573-5036
    Keywords: amides ; free ammonium ; MSX ; net ammonium uptake inhibition ; total free amino acids ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract When N deficient 10-day-old wheat plants were supplied with 1.5 mol m−3 NH4 +, net NH4 + uptake rapidly decreased during the first 6h, while root free-NH4 + and free amino acids concentration increased. However, after 24 h the NH4 + uptake rate increased again, as the internal NH4 + concentration decreased. When plants were pretreated during 40 h with different external NH4 + concentrations, net uptake measured on 1.0 mol m−3 NH4 + decreased with the increasing ion concentration during the pretreatment. This decrement coincided with both root free-NH4 + and total free amino acids levels. When N-starved and NH4 + fed plants were treated during 0, 3 or 6 h with 1.0 mol m−3 NH4 + in the presence of 1.0 mol m−3 MSX2, net uptake (measured without MSX) decreased with the length of the inhibitor treatment. In both groups, MSX significantly increased root free-NH4 + concentration, while the level of total free amino acids was only increased in N-starved plants. When N-starved plants were externally supplied with 1.0 mol m−3 of different amino acids or amides, net NH4 + uptake was only strongly inhibited in the presence of glutamine or asparagine. It is concluded that rapid changes in the concentration of certain amino acids during NH4 + nutrition might regulate the ion absorption, though at high endogenous levels of free NH4 + net uptake could be suppressed independently of the root concentration of free amino acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016286
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  • 198
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    Interaction of salinity and enhanced ammonium and potassium nutrition in wheat (1993)
    Springer
    Plant and soil 154 (1993), S. 133-137 
    Details
    ISSN: 1573-5036
    Keywords: ammonium nutrition ; potassium nutrition ; salinity ; Triticum aestivum L. ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat (Triticum aestivum L.) was grown to maturity in a pot experiment in a calcareous silty sand soil. N was applied at two levels as granulated N-P fertilizers, amended or not with nitrification inhibitors (1% and 5% DCD, 1% N-serve). Potassium as KCl was given at three levels of application. P was applied at a uniform rate. Two levels of salinity were obtained by using the soil as such (EC= 0.3 mmho/cm) and by adding NaCl to the same soil (EC=2.4 mmho/cm). 1% DCD and 1% N-serve treatments gave significantly higher wheat grain yields and N-uptake than the other ones. Nitrate content of leachates indicated a prevalent nitrate nutrition in the treatment without nitrification inhibitors. The 5% DCD treatment showed a yield depression. In the lower N level treatments, a significant yield increase, generated by 1% DCD and N-serve was found in the salinized soil as compared to the non-saline soil. Soil salinity reduced N-uptake when nitrification inhibitors were not present. In treatments having the inhibitors, N-uptake was equal or greater in the salinized than in the non saline soil. An enhanced ammonium nutrition increased the P uptake.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011082
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  • 199
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    Understanding zinc reactions in vertisols to predict zinc responses by wheat (1993)
    Springer
    Plant and soil 155-156 (1993), S. 247-250 
    Details
    ISSN: 1573-5036
    Keywords: rate of Zn desorption ; Vertisols ; wheat ; Zn buffer power ; Zn desorption power ; Zn intensity ; Zn quantity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Since Zn availability to plants growing in a soil is governed by quantity, intensity, buffer power, rate of Zn desorption and diffusion, an improved understanding of a number of these factors in Vertisols would facilitate a more reliable prediction of crop requirements for Zn. The DTPA-extractable Zn, a quantity factor, together with initial Zn desorption rate coefficients, accounted for 80% of the variation in relative dry matter yield of wheat grown to anthesis. In combination with these factors, desorption (buffer) power explained 92% of the variation in Zn concentration in the young mature leaf blade (YMB) of wheat. Thus, the combination of the quantity, rate of Zn desorption and buffer power better predict growth responses of wheat to applied Zn in Vertisols than the commonly-used single extraction with DTPA alone (quantity), which provides only a static measure of Zn availability.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025030
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  • 200
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    The control of boron accumulation by two genotypes of wheat (1993)
    Springer
    Plant and soil 155-156 (1993), S. 305-308 
    Details
    ISSN: 1573-5036
    Keywords: boron ; wheat ; Triticum aestivum L. ; uptake ; genotypic variation ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The mechanism of boron (B) uptake in wheat was studied using two genotypes with known differences in their ability to accumulate B. Influx and efflux of B was measured in the roots of intact 21 d old plants. Roots grown in 15 μM B, when transferred to solutions containing 1mM B showed a rapid increase in B content for up to 60 min, after which no further increase was evident up to 4 h. No genotypic difference in B influx was apparent over these time periods. Roots grown in 1mM B for 7 d and then rinsed in B-free solutions quickly lost most of B that they contained within 1 hour; little further efflux was observed over the following three hours. As with the influx, no genotypic difference in B flux was evident. It is suggested that the lack of genotypic difference in the short-term B fluxes could be due to a masking effect of extracellular B bound in the cell walls of the roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025043
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