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  • Articles  (83)
  • Potassium  (43)
  • Rhizobium  (40)
  • 1980-1984  (83)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (83)
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  • Articles  (83)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 55 (1980), S. 215-224 
    ISSN: 1573-5036
    Keywords: CEC ; Iron ; Manganese ; Nitrogen ; Organic matter ; Paddy ; Phosphorus ; Potassium ; Uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four varieties of each paddy and wheat crops were grown in pots to see their cation exchange capacities of roots and their relationships with the uptake of nutrients by shoot and grain at different physiological growth stages. The results are summarised, as follows: (i) The cation exchange capacity of roots was maximum at tillering stage which continued to decrease with an increase in the age of plants. At early stages of plant growth, significant differences in the root CEC of different varieties of paddy and wheat were recorded but the differences became almost narrow in later stages of plant life and ultimately the CEC of roots became almost similar, in all the varieties as the crops reached to maturity. Higher root CEC of paddy varieties were observed than wheat varieties throughout their physiological growth. (ii) Uptake of phosphorus, potassium, iron and manganese by shoot and grain was found significantly and positively correlated with the CEC of roots in most of the varieties of both, paddy and wheat crops throughout their plant life.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 54 (1980), S. 95-106 
    ISSN: 1573-5036
    Keywords: Acid soil ; Antibiotic resistance ; Competition ; Rhizobium ; Soybean ; Symbiotic nitrogen fixation ; Tropical Africa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The efficacy of inoculating soybean cvs. TGm294-4 and TGm80 in an acid soil (pH 4.6, water) was studied in a field trial in Nigeria.Rhizobium japonicum strains CB1809str r and 46spc r which had been selected for symbiotic competence in the acid soil were used as inocula. Other treatments included no inoculation, combined nitrogen (150 kg N ha−1) and lime (1 t ha−1). Plants grown in acid soil without inoculation and lime had very few nodules, were nitrogen deficient and grain yields were poor (0.3–0.4 t ha−1). Yield responses to combined nitrogen were generally small due to fertilizer loss through leaching. Inoculation greatly increased nodulation, shoot dry matter and shoot N%; grain yields were between 1.6 and 2.0 t ha−1. Lime improved all harvest measurements in the uninoculated and nitrogen treatments but not in the inoculated treatments. In a duplicate trial without lime on near-neutral soil, similar inoculation responses were obtained at 6 weeks. However, at final harvest there were no significant differences in grain yield between treatments due to late, effective nodulation by unidentified strains in the uninoculated treatments. Assays for strain identity confirmed the successful establishment of the inocula in both soils, with 100% of nodules from inoculated plants containing an introduced strain.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 56 (1980), S. 33-37 
    ISSN: 1573-5036
    Keywords: Autochthonous ; Nodule bacteria ; Nodulation ; Plantations ; Rhizobium ; Shelter belts ; Tree legumes ; Wind breaker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Twenty five species of tree legumes, belonging to genera Acacia, Albizzia, Bauhinia, Colophospermum, Dichrostachys, Leucaena, Peltophorum, Pithecolobium and Prosopis were examined for nodulation under pot culture conditions using unsterilized soil. Out of these, only nineteen tree-legumes nodulated. It was interesting to note that all the three species of Bauhinia and one each of Acacia, Colophospermum and Peltophorum did not nodulate both at Jodhpur and Delhi. Inoculation of tree legumes with their own rhizobial isolates, improved nodulation under sterilized conditions.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 56 (1980), S. 491-494 
    ISSN: 1573-5036
    Keywords: Aeschynomene ; Azospirillum ; Rhizobium ; Stem nodules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fifteen isolates of nodule bacteria were isolated from root and stem nodules ofAeschynomene aspera and they were characterized as Rhizobium by well known laboratory tests. All these isolates together with other efficient strains of known rhizobia belonging to different cross-inoculation groups were evaluated for their nodulation abilities onAeschynomene aspera, Cajanus cajan (pigeon pea),Cicer arietinum (chickpea),Pisum sativum (pea),Trifolium repens (clover),Medicago sativa (lucerne),Lens culinaris (lentil),Glycine max (soybean),Vigna sinensis (cowpea),Vigna radiata (mung bean),Vigna mungo (urd bean) andArachis hypogea (peanut). The results demonstrated that Rhizobium fromAeschynomene could form nodules only on its homologous host (Aeschynomene) but not on other legumes tested. Secondly, none of the rhizobia of other cross-inoculation groups could nodulateA. aspera.
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  • 5
    ISSN: 1573-5036
    Keywords: Crop rotations ; Fertilizers ; Lime ; Manures ; Nitrogen ; Nutrient ; Phosphorus ; Potassium ; Removal ; Weedicide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The nett gain or loss of total soil nitrogen was worked out from a long-term manure and fertilizer experiment conducted for seven years and still continuing at Ranchi Agricultural College, Kanke, Ranchi India. The total nitrogen in soil showed a deficit balance where lower and unbalanced doses of fertilizers were applied. Increasing levels of fertilizer combinations with lime removed the highest amount of nitrogen in intensive cropping. The nett loss was highest (95 kg/ha) in case of control, at 100% NPK the nett gain was 37 kg N per ha, and at 150% NPK 72 kg N per ha which was the highest.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 57 (1980), S. 305-321 
    ISSN: 1573-5036
    Keywords: Aluminium ; Acid precipitation ; Calcium ; Calcium carbonate ; Clone ; Iron ; Magnesium ; Manganese ; Nitrogen ; Picea abies ; Phosphorus ; Potassium ; Sulphate ; Sulphur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of acid irrigation on the growth of rooted cuttings ofPicea abies (L.) Karst, was investigated in a pot experiment lasting 3 years. It involved two clones of Norway spruce, H 253 Bogstad I and H 254 Bogstad II. Irrigation water of pH 5.4, 4.0, 3.0 and 2.5 was used. Liming was included in the experiment. After the experimental period, the plants of all treatments were growing reasonably well. However, those plants irrigated at pH 2.5 were slightly discoloured. The plant mortality was only 3% throughout the experiment, and was not connected to acid irrigation. The limiting growth factor was N. All other nutrient elements measured in the plants were close to optimal concentration. Plants irrigated at pH 2.5, and to some extent at pH 3, contained excessively high concentrations of Al, t-S and SO4. The total amount of Ca, Fe and Mn taken up by the plants decreased with increasing soil acidity. The increased growth of clone H 254 relative to H 253, produces a corresponding impression on soil characteristics. Soil acidity is governed by acid irrigation and CaCO3 application, but the clonal effects are also of importance. Norway spruce appears to be tolerant to Al concentrations as high as 50 mmol/kg in the needles.
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  • 7
    ISSN: 1573-5036
    Keywords: Amino acid ; Deficiency ; Keto acid ; Peanut ; Phosphorus ; Potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Lack of both phosphorus and potassuium results in accumulation of all the keto acids and amino acids in 30 days and 20 days old peanut leaves respectively. This is due to te sluggish metabolism of the tissue under P and K deficienty. Decrease of all the keto acids in 20 days old plants suggests that their synthesis might be affecte under P and K deficiency.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 60 (1981), S. 187-193 
    ISSN: 1573-5036
    Keywords: Fertilizer ; Intensive cropping ; Lime ; Multiple cropping ; Manure ; Nutrient removal ; Nitrogen ; Phosphorus ; Potassium ; Weedicide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The balance sheet of phosphorus and potassium were worked out from a long term manure and fertilizer experiment conducted for eight years and is still continuing at Ranchi Agricultural College, Kanke, Ranchi, India. Increasing levels of fertilizer combination with organic manure and lime give the highest yield as well as removed the highest amount of phosphorus and potassium from the soil and gave the positive gain of soil phosphorus and potassium in intensive cropping. The highest gains of 59.0 and 278.0 kg/ha of phosphorus and potassium respectively were recorded in 150% NPK application.
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  • 9
    ISSN: 1573-5036
    Keywords: Bacteroids ; Cowpea ; Nitrogen fixation ; Peanut ; Rhizobium ; Siratro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Acetylene reduction activity and nitrogen accumulation in the plant top per unit nodule mass were compared among peanut, cowpea and siratro plants nodulated by six different strains of Rhizobium. Peanut was found to have several fold higher values than cowpea and siratro for both parameters for all strains of Rhizobium which nodulated it effectively, but the bacteroid content of the peanut nodules was similar to those of cowpea and siratro.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 62 (1981), S. 299-303 
    ISSN: 1573-5036
    Keywords: Beidellite ; Fixation ; Potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary X-ray diffraction studies were made on soils with and without potassium fertility problems. All soils with clay fractions containing dominant beidellite or vermiculite showed potassium deficiency and lack of response to potassium fertilizer applications. All of the soils containing dominant montmorillonite or other clay minerals contained adequate potassium; on none of these, poor potassium response was reported. Special management practices are needed on the beidellitic and vermiculitic soils to increase potassium and ammonium fertilizer efficiency. Dominance of beidellite in the clay fraction should be reflected in soil classification. Establishment of a ‘beidellite’ family differentiating criterion in the Soil Taxonomy is proposed for this purpose.
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  • 11
    ISSN: 1573-5036
    Keywords: Azotobacter ; Glycine max ; Nodulation ; Rhizobium ; Trifolium repens ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Azotobacter vinelandii strains caused the formation of increased numbers of root nodules onGlycine max, Vigna unguiculata andTrifolium repens by their respective rhizobial symbionts. Increased nodulation due to inoculation withA. vinelandii also occurred in field grownG. max. Mutant strains ofA. vinelandii unable to fix nitrogen caused nodulation increases comparable to those caused by nitrogen-fixing strains. This indicates that nitrogen fixation byA. vinelandii was not responsible for the enhanced nodulation. The effect ofA. vinelandii on nodulation was greatest when cells from the mid-exponential phase of growth were applied as inoculants. Non viable cell preparations ofAzotobacter vinelandii were also found to cause an increase in the number of root nodules formed onGlycine max Rhizobium japonicum under greenhouse conditions. The nodulation enhancement activity was influenced by the method chosen to kill theA. vinelandii cells. Heat treatment and treatment with lethal levels of streptomycin destroyed the activity, whereas the activity was unaffected by ultraviolet-light treatment of the cells. Cell-free extracts ofA. vinelandii were found to enhance nodulation. On the other hand, culture supernatants ofA. vinelandii had no effect on nodulation. A split-root experiment suggested that the agent(s) responsible for the increased nodulation was not translocatable throughout the plant. The results suggest a non-excretable protein, produced byA. vinelandii, as a possible mechanism for nodulation enhancement.
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  • 12
    ISSN: 1573-5036
    Keywords: Foliage age ; Nitrogen ; Phosphorus ; Potassium ; Red pine ; Tissue sampling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The vertical distribution of inorganic nutrient concentrations in red pine were dependent on the foliage age. Older foliage did not show an average vertical gradient while younger foliage did show a significant gradient. Horizontal gradients across foliage age classes in a branch whorl were consistent for all branch whorls, but the relative difference between the concentration of the current foliage and foliage three years-old or older was dependent on crown position. Coefficients of variation (CV) did not show a variability gradient in the crown for nitrogen and phosphorus. Variability of potassium tended to decrease as foliage age increased.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 63 (1981), S. 491-495 
    ISSN: 1573-5036
    Keywords: Calcium ; Copper ; Flowers ; Iron ; Leaves ; Magnesium ; Nitrogen ; Nutrient uptake ; Phosphorus ; Potassium ; Koots ; Shoots ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Cucumber (Cucumis sativus) plants were grown in Hoagland solution to which 20 to 2000 ppm of a soil fulvic acid (FA) were added. The addition of 100 to 300 ppm of FA produced highly significant increases in the growth and development of above and below ground plant parts, in the uptake of nutrient elements (N, P, K, Ca, Mg, Cu, Fe and Zn), and in the formation of numbers of flowers per plant. Effects of adding 500 and more ppm of FA were less beneficial.
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  • 14
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    Plant and soil 64 (1982), S. 381-391 
    ISSN: 1573-5036
    Keywords: Atmosphere ; Calcium ; Fertility ; Forestry ; Magnesium ; Nitrogen ; Nutrients ; Phosphorus ; Pinus caribaea ; Potassium ; Savanna ; Sodium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fertility levels in soils beneathPinus caribaea trees were examined in the Mountain Pine Ridge savannas, Belize, where fire control has precipitated the development of pine woodland. Slight surface soil enrichment was recorded beneath pine canopies, but to levels well below those found beneath associated hardwoods. Estimates of total nutrient pools beneath trees showed modest cation accumulation beneath a 73 year old tree but some defecits in Ca and Mg beneath a 24 year old tree. A tap root cutting experiment on trees of the same species revealed no significant declines in foliar nutrient levels after 19 months. It is concluded that no pronounced long-term deterioration in soil fertility levels is developing beneath stands ofP. caribaea in the savanna, although some temporary nutrient declines may exist beneath young pine stands. Atmospheric inputs are the most likely source of nutrient accretion and it is suggested that the establishment of hardwood associates with pine may enhance the rates of nutrient capture from this source.
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  • 15
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    Plant and soil 67 (1982), S. 293-303 
    ISSN: 1573-5036
    Keywords: Burning ; Denitrification ; N-cycling ; N2-fixation ; Nitrification ; Oxisol ; Rhizobium ; Savanna ; South America ; Ultisol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Resumen Las sabanas ocupan alrededor de 300 millones de hectáreas de Sudamérica. Los suelos son básicamente oxisoles y ultisoles de muy baja fertilidad y alta acidez. La vegetación natural varía en densidad y en la cantidad de biomasa producida anualmente, la cual puede llegar a ser igual a la producida por bosques de la región. Entre los microorganismos fijadores de nitrógeno, los únicos bien estudiados son las bacterias del género Rhizobium. En el manejo de la biomasa de estas áreas, es importance considerar la fijación del nitrógeno, como una fuente posible que reemplace al que fué exportado en las cosechas. La nitrificación y la denitrificación en estos, es intensa pero no bien estudiada. La distribución de lluvias durante la estación de crecimiento parece tener una influencia considerable en la provisión de nitrógeno de los suelos. Se registran considerables pérdidas de nitrógeno en este ambiente, cuando amplias áreas son quemadas anualmente.
    Notes: Abstract Savannas cover about 300 million hectares of South America. The soils are mainly oxisols and ultisols and their natural fertility is very low with high acidity. The natural vegetation varies in density and in the amount of biomass produced annually, which can be equal to that produced by forests in the region. Among the nitrogen-fixing micro-organisms, the only ones well-studied are Rhizobium bacteria. In managing the biomass in these areas, it is important to consider biological nitrogen-fixation as a possible source of nitrogen to replace that removed in crops. Nitrification and denitrification in these soils are intense but not well studied. The rainfall distribution during the growing season seems to have a considerable influence of the nitrogen supply to the soils. A considerable loss of nitrogen occurs in this environment when vast areas are burned annually.
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  • 16
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    Plant and soil 69 (1982), S. 105-118 
    ISSN: 1573-5036
    Keywords: Calcium ; Fertility ; Forestry ; Magnesium ; Nitrogen ; Nutrients ; Phosphorus ; Pinus caribaea ; Potassium ; Savanna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Concentrations of P, N, K, Ca, and Mg in above-ground tissues ofP. caribaea were sampled in the species'native savanna habitat. Concentrations were relatively low, but some evidence of higher consumption of K and Ca was found in trees grown on more fertile soils. Regressions were developed to predict the quantities of nutrients sequestered in above-ground tree tissues, and estimates made of the quantities stored in above-ground stands of this species in its native habitat and in several plantations elsewhere. Estimates were also made of the nutrient removals to be expected by harvesting these stands in different ways. Nutrient quantities stored in stands generally exceed those extractable from savanna surface soils, and it is suggested that inputs from the atmosphere are the most probable alternate nutrient source. A comparison of these inputs for tropical areas with the quantities required for stand growth in the savanna, and harvesting removals, suggests that an adequate supply of all elements except P exists, provided that capture by pine is effective. However, atmospheric inputs generally fall below the storage and harvest removal rates for fast growing exotic plantations of this species suggesting that multiple rotations of these plantations at current growth rates may not be feasible without artifical fertilization.
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  • 17
    ISSN: 1573-5036
    Keywords: Aeration ; Calcium ; Carbon dioxide ; Ethylene ; Nitrate ; Nitrite ; Nitrous oxide ; Oxygen ; Potassium ; Roots ; Triticum aestivum ; Waterlogging ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We report a study of the mechanism by which the response of plants to waterlogging can be modified by soil temperature. Wheat was grown initially in well-aerated soil in a controlled environment room before the soil was flooded with aerated, deionized water. The soil temperature was maintained constant in the range 6–18°C while the air temperature was at 14°C. Waterlogging damage was greater in plants at the higher soil temperatures when the plants were compared at the same chronological age. However, when compared at the same growth stage, the response to soil temperature was little differenti.e. plants subjected to waterlogging for a long time at low soil temperatures exhibited a similar reduction in growth and other properties as those subjected briefly at higher temperatures. The concentration of dissolved oxygen in the soil solution declined rapidly at all temperatures, being almost zero after 36 h waterlogging. Temperature affected rates of change of the concentrations of dissolved carbon dioxide, ethylene, nitrous oxide, nitrite, nitrate, calcium and potassium. The importance of soil-and plant-determined properties in the waterlogging response of plants at different temperatures are discussed.
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  • 18
    ISSN: 1573-5036
    Keywords: Neem cake coated urea ; Nitrogen ; Phosphorus ; Potassium ; Rice ; Rice-wheat ; Sulphur coated urea ; Urea ; Urea briquette
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Removal of NPK in the rice-wheat double cropping system was studied in a field experiment conducted for two crop years (1977–79) at the Indian Agricultural Research Institute, New Delhi. The double cropping system producing 8×103 kg/ha/yr grain (4.5×103 kg rice and 3.5×103 kg wheat) removed 166 kg N, 35 kg P and 174 kg K per hectare per year. Thus the rice-wheat double cropping system heavily depletes the soils of their NPK content and calls for balanced fertilization for continued high production. NPK removal was higher when a 135-day duration rice variety was grown than when a 105-day duration variety was grown, because the former resulted in overall (rice + wheat) more grains and straw. Similarly grain and straw yield and NPK removal was more with transplanted rice than when sprouted rice seeds were sown on puddled seedbed; the latter method was in turn superior to direct drilling. Use of urea briquette, neem cake coated urea and sulphur coated urea gave higher yields and resulted in removal of more NPK than prilled urea.
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  • 19
    ISSN: 1573-5036
    Keywords: Calcium ; Nutrient stress ; Phosphorus ; Potassium ; Root growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Techniques developed to measure growth of tomatoes and beans under limiting amounts of either P, K or Ca in solution culture reveal differences among strains. Genetic analysis permits estimates of gene action for control of efficiency and the isolation of improved segregants. The genetic isolates have value in studying mechanisms contributing to uptake, transport and utilization processes.
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  • 20
    ISSN: 1573-5036
    Keywords: Absorption ; Barley ; Excised roots ; Multi-compartment transport box ; Phosphate ; Potassium ; Radioisotopes ; Translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of high concentrations of sodium chloride (NaCl) and polyethylene glycol (PEG) on the absorption and translocation of K and P were examined using a multi-compartment transport box with excised roots of barley. The results were as follows: When no Ca was added, a high concentration of NaCl inhibited the absorption and translocation of K and P, although the inhibition of K was more pronounced as compared with that of P. The inhibitory effect of PEG was smaller than that of NaCl. On the other hand, the drastic inhibition of ion absorption and translocation of P was increased dramatically up to the control level by Ca, even in a high NaCl condition. The results, especially in the presence of Ca, are quite consistent with water culture experiments in the preceding paper15, which reported a less inhibitory effect of salt and water stresses on P absorption.
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  • 21
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    Plant and soil 76 (1984), S. 127-137 
    ISSN: 1573-5036
    Keywords: Adenylate pool ; Biomass volume ; CO2 evolution ; Chitin ; DNA ; Electron microscopy ; Enzymes ; Fluorescent antibody ; Fumigation-respiration ; Fungi Histochemistry ; Imunofluorecence ; Jones-Mollison technique ; Microcosms ; Monoclonal antibodies ; Nitrogen ; Nutrients ; Oxygen consumption ; Phosphorus ; Phytotoxins ; Plate counts ; Rhizobium ; Rhizosphere ; Sulphur ; Xenobiotics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary There is an immense literature on biological and biochemical analyses of soils. Such analyses have revealed the enormous richness of species in soil and their vast range of metabolic potentials and ecological diversity. Accordingly, the approaches used to investigate the soil biota and its biochemistry usually have to be modified or adapted depending upon the purpose of the investigation. Studies of micro-organisms in the soil environment, are complicated because microbial cells are commonly attached to surfaces where they live side-by-side with other populations in consortia usually containing different morphological and physiological types. Such assemblages of organisms cannot be described quantitatively using cultural techniques, such as plate counts, which underestimate both cell numbers and viable biomass. The development of more powerful observational and staining techniques has improved our knowledge of the diverse morphological and biochemical composition of soil micro-communities. Such findings have been amplified at a grosser level by laboratory studies with multi-component systems (microcosms) to mimic field situations and to assess the range of biochemical potentials of microbial consortia. But despite notable advances in analytical methods we are still, with a few exceptions, unable to detect or identify those microorganisms which carry out specific biochemical transformations or determine whether particular cells are alive, dormant or dead at the time of observation. Considerable work has been done to define some of the fundamental ecological attributes of microbial assemblages in soil. Productive work on the metabolic activities of the soil microbiota, specially geochemical transformations of C, N, S and P, has been under way for more than a century. But only in more recent years have more sensitive and reproducible analytical methods become available to measure viable biomass in soil. This will enable some insight to be gained into the role that microbial biomass plays as a labile source and sink for plant nutrients.
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  • 22
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    Plant and soil 77 (1984), S. 387-390 
    ISSN: 1573-5036
    Keywords: Antibiosis ; Culture filtrate ; Fungi ; Peanut ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four strains ofRhizobium sp. from peanut (Arachis hypogaea L.) root nodules were tested for their sensitivity to metabolites (culture filtrates) of more than ten common soil inhabiting fungi, in yeast extract mannitol agar and broth cultures. Among the rhizobial strains tested strain CB-530, BU-1 and BU-2 were not sensitive to metabolites ofMyrothecium roridum andFusarium moniliforme whereas CB-1024 was sensitive. Culture filtrates ofGliocladium roseum, Thielavia basicola andDrechslera pedicellata inhibited the strains CB-530 and BU-2 but not CB-1024. Most of the soil inhabiting fungi tested were inhibitory to rhizobial growthin vitro and very few were stimulatory, their effect in either case being strain specific.
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  • 23
    ISSN: 1573-5036
    Keywords: Acid soils ; Inoculant ; Rhizobium ; Soybeans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Rhizobial inoculation trials were conducted in an acid heavy clay soil in Mekong Delta, Viet Nam, using peat based inoculants produced locally and the commercial granular product of Nitragin CCo., Wisconsin, USA. The pH of these soils ranged from 4.5 to 5.1. Two soybean cultivars, MTD6 and MTD10, were tested as host plants. There were no significant differences between locally made inoculant treated plants and the uninoculated controls in both cultivars. But, the Nitragin inoculation improved all plant characteristics examined in both cultivars. Grain yields of Nitragin inoculated plants of cultivar MTD6 and cultivar MTD10 were 6.5 and 5.5 times as much as those of the controls; protein content of grain increased 11 and 16 percent, respectively. Well nodulated plants had shorter life cycles, flowering durations, and days to flowering. The Rhizobium symbiosis resulted in an additional 153 kg grain-N/ha. These studies show that a surface coated commercial multistrain inoculant can be used to successfully grow soybeans in the acid, heavy clay soils of the Mekong Delta.
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  • 24
    ISSN: 1573-5036
    Keywords: Acid soils ; Nitrogen fertilizer ; Rhizobium ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soybean cultivar MTD10 cultivated on a moderate acid paddy soil of the Mekong Delta responded well to a multistrain inoculation and to chemical nitrogen fertilizer. However, the efficiency of chemical nitrogen uptake by the uninoculated plants decreased with increasing nitrogen application rate. Inoculation improved all examined agronomic characteristics of plants. Inoculated plants produced grain yield 10 times greater than the uninoculated controls and 2.5 times greater than the uninoculated plants receiving 80 kg/ha of chemical nitrogen. Inoculation was superior to chemical nitrogen fertilization in all parameters of importance to market-oriented farmers.
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    Plant and soil 55 (1980), S. 163-165 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Nitrogen fixation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method is described to demonstrate nitrogen fixation by free-living Rhizobium cells. After aerobic growth in a nutrient solution, the bacteria are centrifuged. Acetylene reduction by the rhizobial cells in the pellet can be measured within a few days. Hydrogen gas frequently stimulates acetylene reduction.
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  • 26
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    Plant and soil 74 (1983), S. 395-406 
    ISSN: 1573-5036
    Keywords: Fix− mutants ; Fix+ revertants ; Macroptilium lathyroides ; Nitrogen fixation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Eight ineffective mutant strains were isolated from N-methyl-N'-nitro-N-nitrosoguanidine mutagenized cultures of cowpea Rhizobium strain 32H1. Strains CR1, CR2, CR3, CR4, CR5 and CR6 induced more, but smaller, nodules than the wild type. With the exception of strain CR2, these mutant strains reduced less than 1% of the amount of acetylene reduced by the wild type, in both the free-living and symbiotic assays. Strain CR2 reduced acetylene in the free-living assay but not in the symbiotic assay. Strains CR7 and CR8 responded variably (5–20% of the wild type) in free-living and symbiotic acetylene reduction assays. Nodules also varied from small white to normal-sized pink nodules. The phenotypic characteristics of the mutant strains were consistant with all leguminous plants tested and were stable upon reisolation from nodules. Fully effective revertants were selected from 4 of the ineffective mutant strains by the use of the leguminous plant,Macroptilium lathyroides. Serology, patterns of resistance to anti-bacterial agents, phage-typing, and antibiotic resistance markers were used to confirm strain identification.
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    Plant and soil 64 (1982), S. 129-138 
    ISSN: 1573-5036
    Keywords: Ammonium ; Buffering power ; Nitrate ; Nutrient availability ; Phosphate ; Potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In most arable soils the nitrate availability depends mainly on the quantity of nitrate present in the rooting zone at the beginning of the growing season. Easily mineralizable organic N and the release of non-exchangeable NH4 from clay minerals may in addition control the nitrogen availability during a season. In flooded soils, ammonium is the major form of nitrogen absorbed by plants. Ammonium dynamics in these soils is similar to that of potassium. The availability of both is controlled mainly by the intensity and buffering power for ammonium or potassium, respectively. Basically, intensity of the supply and buffering power for phosphate are the main factors determining the phosphate availability. The determination of the phosphate buffer power, especially in the root zone, however, remains to be difficult. Soil test methods should take into consideration the major factors and processes relevant to the availability of a particular plant nutrient.
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    Plant and soil 64 (1982), S. 393-401 
    ISSN: 1573-5036
    Keywords: Barley ; Cysteine ; Potassium ; Sulfur ; Translocation ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Maximum uptake of Zn in barley (Hordeum vulgare L.) seedlings occurred from nutrient solutions containing SO4−S at 3.5 ppm and K at 6 ppm. Decreased translocation of Zn from roots to tips was observed when plants were grown with lower levels of S and K. Cysteine substituted for SO4-ion as a source of S in Zn absorption, and more Zn was absorbed with cysteine than with sulfate. The effect of K on Zn absorption seems to be influenced by S nutrition in plants.
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  • 29
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    Plant and soil 65 (1982), S. 133-135 
    ISSN: 1573-5036
    Keywords: Green gram ; Nitrogenase-activity ; Pathogenesis ; Rhizobium ; Rhizoctonia ; Specific activity ; Symbiosis ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A pot experiment, using cowpea Rhizobium and one percent inoculum ofRhizoctonia botaticola (Taub.) Butler was carried out on green gram (Vigna radiata cv. PS 16) to study the interaction between symbiosis and root rot in terms of plant growth and nitrogen fixation. The microtomy of the infected roots showed distortion of the outer layers of root as a possible cause for antagonistic interaction of these two bio-processes.
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  • 30
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    Plant and soil 66 (1982), S. 413-416 
    ISSN: 1573-5036
    Keywords: Growth ; Nematicide ; Nitrogen fixation ; Nodulation ; Peanut ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of two granular nematicidesviz. oxamyl and fenamiphos, on the nodulation and growth of Rhizobium inoculatedArachis hypogaea L. was studied in glasshouse and field trials. In the glasshouse trial at the suggested rates of application shoot fresh weight was significantly reduced by oxamyl whilst root fresh weight was similarly affected by fenamiphos. In the field trial vegetative growth and plant emergence were significantly reduced by both nematicides. Nodulation at the higher rates of application was increased by both oxamyl and fenamiphos whilst oxamyl caused a significant increase in pod number at the highest rate of application.
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    Plant and soil 66 (1982), S. 439-442 
    ISSN: 1573-5036
    Keywords: N2-fixation ; Nodulation ; Rhizobium ; Soybean ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of mineral nitrogen on establishment and activity of symbioses between soybean and several strains ofRhizobium japonicum and on the establishment of nodules ofR. japonicum isolated from nodules of field crops is studied. All strains were highly susceptible to the effects of 200 ppm NO3−N on the establishment of symbiosis; 50 ppm NO3−N had little effect. Response of symbioses establishhed in the absence of mineral N to short term exposure to nitrate or ammonium varied significantly between strains. Nodule isolates from soybean crops growing in nitrifying soil were no less susceptible to the inhibitory effects of mineral N on nodule formation than a laboratory culture of the commercial inoculant strain.
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    Plant and soil 67 (1982), S. 227-239 
    ISSN: 1573-5036
    Keywords: Extension ; Inoculant production ; Latin America ; N2-fixation ; Rhizobium ; Training
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Resumen Más de 60 instituciones y 100 investigadores están trabajando de la investigación con Rhizobium en América Latina. La mitad de los investigadores están localizados en Argentina y Brasil. La actividad de investigación y la aplicación de los conocimientos científicos varían ampliamente de acuerdo con los paises. Los problemas de la investigación incluyen: 1) Entrenamiento inadecuado de los investigadores y poca atención para la simbiosis Rhizobium/Leguminosas en las escuelas de Agronomía; 2) investigaciones de baja prioridad sin consideración para las necesidades más inmediatas para los agricultores tales como identificación de los factores limitantes ambientales (por ejemplo: deficiencias nutricionales), técnicas para la producción de inoculantes en pequeña escala y poco control de calidad de los inoculantes disponibles; 3) aislamiento de los investigadores e insuficiente apoyo de literatura; 4) baja interdisciplinaridad en las investigaciones (por ejemplo: en muchas instituciones los investigadores son microbiológos sin conocimientos de agronomía, o agrónomos sin entrenamiento en microbiología y 5) insuficiente diseminación de los conocimientos científicos. Los problemas de la producción y control de inoculantes incluyen: 1) las cepas empleadas en los inoculantes (nacionales o importadas) no son seleccionadas localmente; 2) poco control de calidad de los inoculantes y como resultado, inoculantes malos traen descrédito para la práctica de la inoculación, y 3) precios muy altos de los inoculantes. Los problemas de la extensión incluyen: 1) falta o deficiencia de los programas de promoción de leguminosas por las organizaciones gubernamentales, 2) poco contacto entre los investigadores y los extensionistas y 3) administradores líderes, extensionistas y agrónomos que trabajan en el campo no poseen adecuados conocimientos sobre la simbiosis Rhizobium/Leguminosas. Algunas medidas inmediatas para promover la extensión y programas de promoción de las leguminosas y/o control oficial de la calidad de los inoculates son necesarias en Argentina, Uruguay, Brasil, México y posiblemente Colombia. Perú, Venezuela, Costa Rica y Chile necesitan esfuerzos combinados dirigidos prioritariamente para promover la investigación y extensión. En Ecuador, Paraguay, Bolivia, Nicaragua, Honduras, Guatemala, República Dominicana y Panamá, la prioridad debe ser dada para la investigación. Colombia debe ser incluída en este grupo por la razón de que las instituciones nacionales deben ser fortalecidas. La tabla 2 relaciona estas prioridades con mas detalles.
    Notes: Abstract More than 60 institutions and 100 researchers were involved in Rhizobium research in 1978 in Latin America. Half of these researchers were located in Argentina and Brazil. Research activity and the application of research findings vary widely among countries. Problems that plague research include 1) inadequate training of research personnel and insufficient attention paid to the Rhizobium/Legume symbiosis at agriculture schools; 2) poorly-established research priorities that do not sufficiently weigh the immediate needs for the farmers such as the identification of limiting environmental factors (e.g. nutritional deficiencies), techniques for smallscale inoculant production, and quality control of available inoculants; 3) isolation of the researchers and a lack of adequate library support; 4) poorly integrated research teams (e.g. in many institutes researchers are either microbiologists with no agricultural background or agronomists lacking microbiological training); and 5) insufficient dissemination of research findings. Problems with inoculant production and control include 1) a local dependence on national or imported inoculants rather than on locally-selected strains, 2) poor inoculant quality control which results in low inoculation success rates and subsequent discredit to the inoculation practice, and 3) high prices for inoculants. Extension problems include 1) lacking or deficient legume-promotion programs by government agencies, 2) poor contact between research and extension workers, and 3) administrators, leaders, extension workers and agronomists working in the field that lack adequate knowledge of the Rhizobium/Legume symbiosis. Immediate measures to foster extension and legume promotion programs and informal and/or official quality control are needed in Argentina, Uruguay, Brazil, Mexico, and probably Colombia. Countries where combined efforts should primarily be directed toward stimulating research and extension include Peru, Venezuela, Costa Rica, and Chile. In Ecuador, Paraguay, Bolivia, Nicaragua, Honduras, Guatemala, the Dominican Republic and Panama, priority should be given to research. Colombia should also be included in this group as national research institutions need to be strengthened. Table 2 lists these priorities more fully.
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  • 33
    ISSN: 1573-5036
    Keywords: Glycine max ; Iron deficiency ; Mycorrhizae ; Nodulation ; Platte ; Valley ; Yellows ; Rhizobium ; Soybeans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A chlorotic condition of soybeans in the river valleys of eastern Nebraska was studied. The pattern of chlorosis, presence of petiolar necrosis, reductions in rhizobial and mycorrhizal infection, and plant and soil chemical analyses suggest that the condition is more complex than normal iron deficiency chlorosis or other mineral deficiencies or toxicities. Nodulation is almost totally inhibited in affected plants. Mycorrhizal fungi, while able to infect early in the season, do not persist on affected plants. Breakdown of endodermis was observed in the roots of affected plants. The factors contributing to this condition have not yet been determined.
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  • 34
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    Plant and soil 69 (1982), S. 163-169 
    ISSN: 1573-5036
    Keywords: Biodegradation ; Catechin ; p-Hydroxybenzoate ; Oxidation ; Rhizobium ; Salicylate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The ability of rhizobia to utilize catechol, protocatechuic acid, salicylic acid, p-hydroxybenzoic acid and catechin was investigated. The degradation pathway of p-hydroxybenzoate byRhizobium japonicum, R. phaseoli, R. leguminosarum, R. trifolii andRhizobium sp. isolated from bean was also studied.R. leguminosarum, R. phaseoli andR. trifolii metabolized p-hydroxybenzoate to protocatechuate which was cleaved by protocatechuate 3,4-dioxygenasevia ortho pathway.R. japonicum degraded p-hydroxybenzoate to catechol which was cleaved by catechol 1,2-dioxygenase.Rhizobium sp., a bean isolate, dissimilatedp-hydroxybenzoate to salicylate. Salicylate was converted to gentisic acid prior to ring cleavage. The rhizobia convertedp-hydroxybenzoate to Rothera positive substance. Catechol and protocatechuic acid were directly cleaved by the species.R. japonicum converted catechin to protocatechuic acid.
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    Plant and soil 69 (1982), S. 265-273 
    ISSN: 1573-5036
    Keywords: Azotobacter chroococcum ; Fertilizers ; Farmyard manure ; Nitrogen ; Nitrogen fixation ; Potassium ; Phosphorus ; Yields of grain stover ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field trials were conducted during theKharif seasons of 1979 and 1980 to examine the effect of the nitrogen-fixing bacteriumAzotobacter chroococcum (isolate M4) on yields of maize and N-economy. Different levels of nitrogen and farmyard manure (FYM) were supplied to assess their interaction with Azotobacter inoculation. Seed inoculation without fertilization raised grain and stover yields significantly. FYM coupled with inoculation gave higher yields than either could singly. The financial gain due to an increase in grain yield upon applying per ha 80 kg N, 10×103 kg FYM and Azotobacter over the treatment 40N + FYM + Azotobacter was offset by the cost of the additional N; thus, the higher N-application was not economical. Azotobacter inoculation was economically most efficient at lower doses of fertilizer nitrogen which not only increased yields but resulted in a saving of fertilizer N when applied in combination with FYM.
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    Plant and soil 69 (1982), S. 275-280 
    ISSN: 1573-5036
    Keywords: Azotobacter chroococcum ; Fertilizers ; Farmyard manure ; Zea mays ; Maize ; Nitrogen ; Nitrogen fixation ; Phosphorus ; Potassium ; Total nitrogen uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The economic feasibility of using cultures of nitrogen fixing microorganisms in programmes to increase crop production, as a selfgenerating source of nitrogen, has been proved beyond doubtviz. Legume-Rhizobium symbiosis and blue green algal ‘fertilizer’ for rice. The extent to which the free living, N-fixing, aerobic, heterotropicAzotobacter chroococcum could replace the application of nitrogenous fertilizer to maize was investigatedin vivo. Total nitrogen uptake (kg ha−1) by maize after inoculation with Azotobacter combined with moderate applications of nitrogen fertilizer and farmyard manure was influenced significantly and resulted in a higher nitrogen concentration in grain and stover along with a higher yield.
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    Plant and soil 70 (1983), S. 77-93 
    ISSN: 1573-5036
    Keywords: Adsorption ; Calcium ; Calcium carbonate ; Cation Exchange Capacity ; Hydrogen ; Magnesium ; Moss ; pH ; Potassium ; Sodium ; Soil preference
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Moss species with a different soil preference with respect to its acidity and carbonate content also differ in their cation exchange capacity (leaf, stem, rhizoid), the acidiphile-calcifuges having lower C.E.C. values than the neutrophile-calcicoles. The higher the C.E.C. value, the more the divalent cations calcium and magnesium tend to be adsorbed as compared to the monovalents sodium and potassium, from an equinormal tetracationic solution. The increase in divalent adsorption is mainly due to calcium. Considering this differential adsorption of calcium and in view of data in literature on membrane characteristics, it appears that among the studied cations calcium is an important factor in the observed soil preference through its presence in the cell wall adsorbed fraction and thus in the membrane environment.
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  • 38
    ISSN: 1573-5036
    Keywords: Alkali metals ; Alkaline earth metals ; Barium ; Calcium ; Ceasium ; Lithium ; Magnesium ; Potassium ; Rubidium ; Sodium ; Strontium ; Temperate forest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Absorption and accumulation of alkali (Li, Na, K, Rb, Cs) and alkaline earth (Mg, Ca, Sr, Ba) metals were investigated as taxonomic characteristics (in 62 plant species). Leaf and soil samples were collected from 9 sites in temperature forest in Japan and the above mentioned elements were analyzed. Considerable differences were found among species in their ability to accumulate alkali and alkaline earth metals. Very high concentrations of Li (45 ppm, D.W.), K (37×103 ppm), Rb (159 ppm) and Cs (8.2 ppm) were detected inLastrea japonica which were about 412, 12, 27 and 6 times higher than those of the species with the lowest concentrations. Na content was high inAcer micranthum (358 ppm) which was 16 times higher than species with the lowest concentration. Other species containing high levels of alkali metals wereHydrangea macrophylla, Struthiopteris niponica, Clethra barbinervis. Mean discrimination ratio (D.R.) for all investigated plant species for Li, Na, Rb, and Cs to K were 1.7, 0.44, 0.9 and 1.8 respectively. High concentrations of alkaline earth metals Ca (36×103 ppm), Sr (345 ppm), and Ba (241 ppm) were found in the leaves ofHydrangea paniculata which were about 31, 84, and 72 times higher than those for the species with the lowest concentration. Mg was very high inStruthiopteris niponica (83×102 ppm). Other species with high concentrations of alkaline earth metals belonged to the genus Viburnum. Mean D.Rs. for Mg, Sr, and Bavs Ca were 1.0, 0.7 and 0.08. Principal component analysis of interrelationships between the mineral content in leaf tissues indicated that these elements could be classified into 2 groups with respect to their accumulation behavior in plants. The alkali metals K, Li, Rb, and Cs behaved similarly in their accumulation in leaves but Na behaved independently. Alkaline earth metals Ca, Mg, Sr, and Ba were also found to behave similarly in their accumulation. Factors scores of 1st and 2nd components revealed three groups of plant species: alkaliphilic, alkaline earthphilic, and neutral (non-accumulators).
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    Plant and soil 70 (1983), S. 309-316 
    ISSN: 1573-5036
    Keywords: Calcium ; Ionic interaction ; Magnesium ; Nutrient solution ; Oryza sativa L. ; Phosphorus ; Potassium ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ionic interactions may occur as cation-cation interactions, anion-anion interactions, or cation-anion interactions. Greater knowledge of this subject is warranted, due to the intensification of agriculture, in devising efficient fertilization systems. The present study was undertaken to elucidate the interaction among P, K, Ca and Mg absorption by the intact rice (Oryza sativa L.) plants from dilute solutions. The uptake of P was independent of Ca concentrations up-to 250 μM Ca but-there was a small decrease at the higher concentrations. Absorption of K and Mg was stimulated in the presence of Ca ions at low concentrations. But K and Mg absorption was decreased at higher Ca concentrations. Increasing concentrations of K in the nutrient solution depressed P and Ca uptake. Magnesium uptake was stimulated at lower concentrations of K, but at higher concentrations it was also decreased. Similarly, absorption of K and Ca was also decreased with increasing concentrations of Mg. Magnesium at lower concentrations stimulated P uptake but at higher concentraions it was decreased. In the present study maximum growth of rice plants was achieved at about 250μM Ca, 260 μM K, and 33μM Mg in the nutrient solution.
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  • 40
    ISSN: 1573-5036
    Keywords: Boron ; Calcium ; Copper ; Genotypic differences ; Iron ; Magnesium ; Manganese ; Mineral nutrition ; Molybdenum ; Nitrogen ; Phosphorus ; Plant adaptation ; Plant breeding ; Potassium ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant genotypes differ in their uptake, translocation, accumulation, and use of mineral elements. Examples of genotype differences to iron, nitrogen, phosphorus, potassium, calcium, magnesium, manganese, boron, copper, zinc, and molybdenum are discussed. Current knowledge is sufficient to indicate that many crop plants can be improved for the efficient use of mineral elements and better adaptation to mineral stress conditions.
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    Plant and soil 71 (1983), S. 371-380 
    ISSN: 1573-5036
    Keywords: Apple ; Calcium ; Copper ; Iron ; Irradiance ; Magnesium ; Manganese ; Nitrogen ; Nutrient uptake rate ; Phosphorus ; Potassium ; Scion effects ; Season ; Source of nitrogen ; Temperature ; Tissue nutrient level ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The rates of uptake of nutrients from solution by apple roots were measured (a) in a root laboratory, using intact roots of mature trees growing under field conditions and (b) in controlled environment using young trees. Maximum nitrate inflows into Discovery/M.9 roots under field conditions were only slightly lower than those into roots of the same genotype in controlled environment, but up to 80 times lower than those into roots of Worcester Pearmain seedlings. At any given external P concentration, P inflows into roots of field-grown trees were about 2.5-times lower than those into the roots of young trees in controlled environment. Nitrate inflows were constant above a solution concentration of 20 mmol m−3 in both field-grown and small trees. In both cases, phosphate inflows increased linearly with solution concentration up to 10 mmol m−3. Among the various plant and environmental factors influencing nutrient uptake characteristics of apple roots were: the scion genotype, tissue nutrient levels, root origin, the form in which N is supplied, level of irradiance of the shoot, root temperature and the season of the year. The effects of these factors are illustrated with examples.
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  • 42
    ISSN: 1573-5036
    Keywords: Corn ; Nitrogen ; Phosphorus ; Potassium ; Sugarbeet ; Sunflower
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A three-year experiment was conducted in natural conditions on chernozem soil to examine the efficiency of nitrogen, phosphorus, and potassium use by corn (C4 type), sunflower and sugarbeet (C3 type) grown in optimum conditions of mineral nutrition (N100P100K100 kg/ha). Plant materials were analysed for the concentration of nitrogen, phosphorus, and potassium and dry matter mass per individual plant parts and the whole plant. Leaves of different age, of all three plant species, were analysed to find eventual differences in the efficiency of use of nitrogen, phosphorus, and potassium in the synthesis of organic matter depending on leaf age. It was found that corn had the lowest concentration of the elements studied but the highest dry matter mass. In other words, corn was more efficient than sunflower or sugarbeet in the use of these elements for the synthesis of an organic matter unit. Such results were arrived at in both sets of analyses, i.e., the analyses of leaves performed in the course of ontogenetic plant development as well as the analyses of leaves of different age.
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  • 43
    ISSN: 1573-5036
    Keywords: Calcicole ; Calcifuge ; Calcium ; Dittrichia viscosa ; Magnesium ; Potassium ; Sodium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Dittrichia viscosa is a Mediterranean bush widespread on various soil types. It is shown that the plants from a calcareous habitat (G plants) and those from an acidic habitat (M plants) differ in their ability to accumulate various cations when growing in the same experimental conditions. On this acidic soil the G plants accumulate more Ca and less K than the M plants. On a calcareous soil the response is reversed; it is the M plants which contain more Ca and often less K. This behaviour on each of the soils is typically that of a calcifuge for M and a calcicole for G. The two types of plants also differ in their affinity for Mg and Na. The ubiquity of the species could well be explained at least partially by genetic differentiation in the ability of plants to select ions.
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  • 44
    ISSN: 1573-5036
    Keywords: Acetylene ; CO inhibition ; Conversion ratio C2H4/N2 ; Effect oxygen ; Hydrogen evolution ; 15N2 fixation ; δ15N measurements ; Parasponia parviflora ; Relative efficiency nitrogen fixation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Isotopic15N2 experiments confirmed nitrogen fixation inParasponia parviflora. The conversion ratio C2H4/N2 was 6.7 under the experimental conditions employed. Measurements of the δ15N in leaves of Parasponia and Trema showed on the basis of these determinations thatParasponia parviflora possesses N2-fixing capacity and can be distinguished in this respect from the non-nitrogen-fixingTrema cannabina tested by the same method. Therefore, δ15N can be used to monitor N2 fixation in natural ecosystems. Hydrogen evolution and the relative efficiency of N2 fixation in this relation have been determined. DetachedParasponia parviflora root nodules grown in soil and tested in an argon/oxygen atmosphere produced appr. 4 μmol H2.h−1.g−1 fresh weight root nodules. The relative efficiency of hydrogen utilization as measured in argon, air, and in the presence of C2H2 10% (v/v) was for both equations $$\left( {1 - \frac{{H_2 (air)}}{{H_2 (Ar)}}} \right) and \left( {1 - \frac{{H_2 (air)}}{{C_2 H_2 }}} \right)$$ used for to express this efficiency 0.96 and 0.97, respectively. This indicates that Parasponia like the root nodules of some actinorhizal symbioses (Alnus, Myrica, Elaeagnus) and some tropical legumes (Vigna sinensis) has evolved mechanisms of minimizing net hydrogen production in air, thus increasing the efficiency of electron transfer to nitrogen. The oxygen relation of nitrogen fixation (C2H2) inParasponia parviflora root nodules was determined. The nitrogenase activity of Parasponia root nodules increased at increasing oxygen concentrations up till c. 40% O2. At oxygen levels above 40% O2, the nitrogenase activity of the root nodules was nil or very erratic suggesting that at these oxygen levels the nitrogenase is not longer protected against the harmful effect of oxygen. In this respect Parasponia root nodules differ from actinorhizal root nodules in other nonlegumes, where optimal nitrogenase activity was observed in the range of 12–25% oxygen. Respiration experiments with Parasponia root nodules showed that in the range 10, 20, and 40% oxygen, the respiration rate (CO2 evolution) increased concomitantly with an increase of the acetylene reduction rate. The CO2/C2H4 values obtained varied between 8.1 and 19.2, being therefore 2–3 times higher than similar estimations in some actinorhizal and legume root nodules. The respiratory quotient (RQ) of detachedParasponia parviflora root nodules was in air initially approximately 2.0, but this value dropped to about 1.0 in a 3-hours period.
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  • 45
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    Plant and soil 77 (1984), S. 73-86 
    ISSN: 1573-5036
    Keywords: Fertiliser ; Foliar analysis ; Nitrogen ; Phosphorus ; Pinus radiata ; Potassium ; Soil organic matter ; Steenbjerg effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Copper deficiency inPinus radiata in Gippsland, Victoria was found to be associated with acid, organic-rich sandy podzols. A trial designed to find a Cu supplement for P and NPK fertilisation on these soils showed significant growth in stem-length following Cu-solution treatment in the nursery, and Cu fertiliser applied in the field up to at least 13.5 kg Cu ha−1. The Steenbjerg effect was evident in foliar concentrations of nutrients following fertilisation; this makes diagnosis from foliar analysis difficult. Foliar Cu∶N ratios proved a more sensitive indicator of Cu fertiliser treatment than either Cu or N alone. Two phases of seasonal drainage and podzol development were identified in the experiment. Trees growing on the better-drained, more acid soil with a high content of organic matter responded better to Cu fertiliser.
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  • 46
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    Plant and soil 74 (1983), S. 437-450 
    ISSN: 1573-5036
    Keywords: Burning controlled ; Calcium ; Carbon ; CEC ; Eucalyptus ; Magnesium ; Nitrogen ; N. mineralisation ; Potassium ; Rainforest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In mixed eucalypt/rainforest in southern Tasmania, samples of surface soil 0 to 2 cm, 2 to 5 cm, and 5 to 10 cm were taken from a clear-felled coupe before and after burning in 1982, from a similar coupe after burning in 1979, and from an uncut area adjacent to each coupe. Factors compared were bulk density; total organic C, N, P, Ca, Mg, and K; pH; exchangeable Ca, Mg, and K; cation exchange capacity; extractable P; and N-mineralisation rates. The effect of burning was found to be restricted mainly to the upper 2 cm of soil. The combustion of organic matter caused losses of 7360 kg organic C and 211 kg N/ha; 348 kg Ca and 282 kg Mg and 151 kg K/ha were added to the soil in ash. Burning caused significant increases in pH, exchangeable Ca, Mg, and K, and in extractable P; cation exchange capacity was reduced. In the 6 months after burning only K was leached from the upper 2 cm of soil. Equilibrium levels of NH4−N increased initially after the fire, but between 6 and 18 months, equilibrium levels and rate of production of NH4−N during anaerobic incubation in soil of burned coupes differed little from that in adjacent uncut forest. Rates of production of NO3−N during aerobic incubation were very low throughout the period of study. It is concluded that for soils developed on dolerite in mixed eucalypt/rainforest, a single regeneration burn probably improves the nutritional status of the soil. Nutrients lost from the area as particulate ash are in quantities that will probably be replaced in rainfall in 15 to 20 years.
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  • 47
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    Plant and soil 55 (1980), S. 269-281 
    ISSN: 1573-5036
    Keywords: Ammonium-vs nitrate-N ; Conifers ; Fertilization ; Deficiency ; Greece ; Nitrogen ; Nutrition ; Phosphate ; Pinus halepensis ; P. maritima ; P. radiata ; Potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In conifer fertilization and nutrition experimentsPinus halepensis, P. radiata andP. maritima seedlings were grown in pots, filled with soil derived from mica schist and siliceous tertiary deposits and also in peat substrate in paperpots. Fertilization with P ofP. radiata andP. maritima seedlings growing in soil low in available P and N improved seedling height only in combination with N fertilization and fertilization with alone induced P deficiency symptoms. N fertilization with from 100 to 150 ppm (2.4 to 3.2 g N/kg, respectively) in the soil regardless of the form of N (NH4 + or NO3 −) applied in the summer or autumn together with application of 20 ppm P before sowing was the fertilization regime which produced the best seedlings. Fertilization of peat before sowingP. halepensis, P. radiata andP. maritima with omission of one of the nutrients N, P and K resulted in visible symptoms of N, P and K deficiency, respectively, in the seedlings. Comparative chemical analysis of needles from the three kinds of conifer seedlings with deficiency symptoms and healthy ones verified the visual symptoms of N and P deficiency but not so convincingly the K deficiency symptoms.
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  • 48
    ISSN: 1573-5036
    Keywords: Ammonium acetate-lactate (AL) method ; Electro-ultrafiltration ; Magnesium ; Potassium ; Soil analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of increasing potassium and magnesium fertilization during 15 years on the amounts of K and Mg extracted by AL-solution and desorbed by Electro-Ultrafiltration (EUF) was determined in four Swedish soils. In all soils, with increasing potassium fertilization the amounts of K extracted by AL and desorbed by EUF increased, and the amounts of Mg-AL and Mg-EUF decreased. Magnesium fertilization had no significant effect on the K values but increased both Mg-AL and Mg-EUF. The EUF-values were lower than the corresponding AL-values, but the ratio of EUF-desorbed to AL-extracted amounts of K and Mg varied depending on soil type as well as on fertilization rate. The ratio of K-EUF to K-AL increased and the ratio of Mg-EUF to Mg-AL decreased with increasing potassium fertilization, whereas magnesium fertilization decreased the ratio of Mg-EUF to Mg-AL.
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  • 49
    ISSN: 1573-5036
    Keywords: Dry-matter production ; Potassium ; Potato ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Field experiment was conducted during 1980–81 on the Indo-Gangetic alluvial soil of Pura, Kanpur to study the effects of added potassium and zinc on dry-matter production and uptake of these nutrients by potato crop. Increasing supply of potassium and zinc significantly increased the dry-matter production and concentration and uptake of respective nutrients in different crop components. The effects of combined application of potassium and zinc was positive in influencing the dry-matter production and K, Zn uptake by potato crop. Increasing supply of potassium showed beneficial effect on the absorption and translocation of zinc in plant system indicating thereby greater utilization of fertilizer zinc by the crop. Increasing supply of zinc, however, could not influence the concentration of potassium.
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  • 50
    ISSN: 1573-5036
    Keywords: Isotope techniques ; Nitrogen-15 ; Nitrogen fixation ; Nitrogen utilization ; Phaseolus vulgaris ; Rhizobium ; Varietal comparison
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Differences in N2-fixation byPhaseolus vulgaris bean cultivars were successfully evaluated in the field using15N isotope dilution technique with a non-fixing test crop of a different species (wheat). The Phaseolus cultivars could have been similarly ranked for N2-fixation capacity from either seed yield or total nitrogen yield, but the isotope method provided a direct measure of N2-fixation and made it possible to estimate the proportion of fixed to total nitrogen in the crop and in plant parts. Amounts of nitrogen fixed varied between 24.59 kg N/ha for the 60-day cultivar Goiano precoce to 64.91 kg N/ha for the 90-day cultivar Carioca. The per cent of plant nitrogen due to fixation was 57–68% for the 90-day cultivars and 37% for Goiano precoce (60-day cultivar). Fertilizer utilization was 17–30% of a 20 kg N/ha fertilizer application. 100 kg N/ha fertilizer application decreased N2-fixation without suppressing it totally. Differences in yield between the highest yielding (Carioca) and the lowest (Moruna) 90-day cultivars were also due apparently to varietal differences in efficiency of conversion of nitrogen to economic matteri.e. seed, as well as to differences in capacity of genotypes for N2-fixation.
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    Plant and soil 65 (1982), S. 425-428 
    ISSN: 1573-5036
    Keywords: Chlorosis ; Cowpea ; Nodulation ; Rhizobitoxine ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary While screening cowpea rhizobia from West Africa for ability to nodulate various host species, foliar chlorosis was observed in young mung bean and soybean plants inoculated with certain strains. The chlorosis occurred in the first and sometimes the second trifoliate, but not on subsequent leaves. There was no correlation of symptoms with the presence of nodules. Where extreme chlorosis was induced in soybeans, there was stunting of the primary root. Disease symptoms were obtained with culture-broth supernatants free of rhizobia, indicating an extracellular toxin. In common with rhizobitoxine-producing strains ofR. japonicum, chlorosis-inducing cowpea strains were able to nodulate ‘non-nodulating’ soybeans of the rj1rj1 genotype.
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  • 52
    ISSN: 1573-5036
    Keywords: Breeding ; Cowpea ; Nitrogen nutrition ; Rhizobium ; Seed yield ; Symbiotic interactions ; Vigna unguiculata L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plant of cowpea (Vigna unguiculata (L.) Walp.) cv. TVu 1469 were grown in a plastic house set to simulate tropical temperatures. They were inoculated with one of two strains of Rhizobium and irrigated each day with nutrient solution either devoid of inorganic nitrogen (N) or containing 2.14 mM (30 ppm) N. Strain of Rhizobium significantly affected rates of dry matter and N accumulation as well as the total N content of mature plants. Variations in seed yield were due largely to Rhizobium effects on peduncle production and pod set on each peduncle, wheres inorganic N did not change these yield-determining components significantly. The agronomic and physiological implications of these data are discussed.
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  • 53
    ISSN: 1573-5036
    Keywords: Clipping stress ; Dichanthium annulatum ; Echinocloa colonum ; Grasses ; Moisture content ; Nitrogen Phosphorus ; Polypogon monspeliensis ; Potassium ; Soil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The concentration, uptake and element use efficiency of N, P and K in one C3 annual (Polypogon monspeliensis) and two C4 (Echinochloa colonum, an annual, andDichathium annulatum, a perennial) grasses were determined during winter and summer seasons in monocultures raised in field plots at three moisture levels,viz. full, half and one-fourth of field capacity. At each moisture regime the plants were clipped thrice at moderate and severe levels corresponding to 40 and 80% of live green. The concentration of these elements was characteristic of the growth habit of these plants;e.g. the build up of concentration was maximum in leaf of the annuals while it was comparable in crown and leaf of Dichanthium. The N level was maximum in Polypogon. The nutrient use effiency was comparable in the two annuals and maximum K and N use were obtained in Polypogon and Dichanthium, respectively.
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  • 54
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    Plant and soil 70 (1983), S. 147-150 
    ISSN: 1573-5036
    Keywords: Aluminium ; Arachis hypogea ; Chlorose ; Nématode ; Rhizobium ; Sénégal ; Scutellonema cavenessi ; ‘Yellow patches’
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Summary The authors' analysis of Drevon and Diabaye's article on “yellow patches’ disease of peanuts in Sénégal3 underlines the weakness of the latter's conclusions which tended to attribute this disease to a combination of low soil pH, presence of exchangeable aluminium and a low number of Rhizobium. Taking into account these factors, the authors advance a more complete and better founded explanation which attributes a fundamental role to the nematodeScutellonema cavenessi, a role which has been demonstrated in many previous field and laboratory studies.
    Notes: Résumé Les auteurs, analysant l'article de Drevon et Diabaye concernant la maladie des ‘taches jaunes’ de l'arachide au Sénégal3, relèvent la faiblesse des conclusions; celles-ci tendent à attribuer cette maladie à la conjonction d'un pH bas des sol, la présences d'aluminium échangeable et le faible volume de Rhizobium. Sans négliger ces éléments, les auteurs avancent une explication plus globale et mieux fondée, dans laquelle l'action du nématodeScutellonema cavenessi reste primordiale, ainsi que de nombreuses observations et expérimentations l'ont, antérieurement, démontré.
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  • 55
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    Plant and soil 72 (1983), S. 197-212 
    ISSN: 1573-5036
    Keywords: Allium ; Atriplex ; Cation selectivity ; Fagopyrum ; Halophytes ; Helianthus ; Hordeum ; Plasmalemma fluxes ; Potassium ; Potassium-sodium selectivity ; Salt tolerance ; Secale ; Sodium ; Suaeda ; Tonoplast fluxes ; Triticum ; Xylem parenchyma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In this short survey differences between species and varieties in the four major mechanisms that affect selective uptake of potassium and sodium to the plant within the root are considered. These include influx selectivity, K+/Na+ exchange at the plasmalemma, and selectivity at the tonoplast as well as at the symplasm-xylem boundary. The affinity of various plants for potassium influx in system 1 is rather uniform although varietal differences in barley have been observed. Differences are much more pronounced for sodium influx, for which Helianthus showed rather high and Fagopyrum rather low affinity. There is substantial variation between species in the efficiency of K+/Na+ exchange at the plasmalemma of cortical root cells; the three cereals Hordeum, Triticum, and Secale were highly efficient while K+/Na+ exchange in Atriplex, Helianthus and Allium was poor, even if the cytoplasmic sodium content was accounted for. Apparently there was no direct relation between salt tolerance and K+/Na+ exchange. The observed differences in the efficiency of K+-dependent sodium extrusion or K+/Na+ exchange were not due to the use of excised roots, they were observed also when roots of whole seedlings were investigated. At the tonoplast a 1∶1 exchange of vacuolar potassium for sodium has been observed in roots of Hordeum. By this exchange sodium ions are removed from the symplasm and potassium ions are recovered from vacuoles and thus made available for transport to the shoot. Indications for specific differences in this exchange have been observed; the exchange appears to be more efficient in Helianthus than in Hordeum roots. More comparative studies are needed here. At the boundary between symplasm and xylem vessels selectivity can be set up during xylem release of cations and there are reports that suggest a preference for sodium (Lycopersicum cheesemanii, Solanum pennellii, and Suaeda) and for varietal differences amongst tomatoes. Selectivity at this boundary, the plasmalemma of the xylem parenchyma cells was described in this paper by the selectivity ratio of transport that relates the rates of xylem transport to the cytoplasmic sodium and potassium concentrations. Based on this ratioAtriplex hortensis was shown to discriminate for sodium during xylem release while there was little selectivity in Hordeum and possibly some discrimination in favour of K+ in Allium roots. The data are shortly discussed in relation to salt tolerance and to the breeding of salt-tolerant crop varieties.
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    Plant and soil 73 (1983), S. 17-26 
    ISSN: 1573-5036
    Keywords: Azote ; Croissance ; Nutrition ; Picea abies ; Plantation ; Potassium ; Sol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Abstract A nutrient-growth study was undertaken in 94Picea abies plantations, ranging in age from 20–31 years, in the L-3 forest region, Canada. Correlation analysis and canonical variable analysis indicated a positive relation between growth and the percentage of nitrogen and potassium in needles of the current year growth. By stepwise discriminant analysis, these two elements classify correctly 53% of the sites in the predetermined growth classes whereas combining all foliar characteristics tested classified correctly 59% of the sites. Adequate nitrogen and potassium nutrition is observed on plantations grown on soils with high levels of exchangeable cations and rich in silt and clay.
    Notes: Résumé Une étude nutrition-croissance a été réalisée dans 94 plantations dePicea abies, âgées de 20–31 ans, établies dans la région forestière L-3, Canada. Des relations positives ont été mises en évidence, par l'analyse de correlation et l'analyse des variables canoniques, entre la croissance et les teneurs en azote et en potassium des aiguilles de la pousse courante. Ces deux éléments permettent, par analyse discriminante progressive, de classer correctement en moyenne 53% des stations, au sein des classes de croissance prédéterminées alors que le total des caractéristiques foliaires analysées classe correctement 59% des stations. Une bonne nutrition en azote et en potassium est observée dans les plantations sur sols présentant une teneur élevée en cations échangeables et riches en limon fin et en argile.
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  • 57
    ISSN: 1573-5036
    Keywords: Hill soils ; Lime ; Mycorrhiza ; Nitrogen fixation ; Nodulation ; Phosphorus ; Rhizobium ; Symbioses ; White clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The responses of white clover (cv NZ Grasslands Huia grown in four UK hill soil types) to additions of lime and P, to inoculation with Rhizobium and mycorrhizal fungi, and to differences in soil water status were assessed in pot and field experiments. With a deep peat soil in pots, shoot production, nodulation and N fixation by clover were increased by 160, 130 and 85% respectively following inoculation with mycorrhiza, but in the field, despite a doubling of root infection, there was no response in growth. On a brown earth soil in the field inoculation with one endophyte (Glomus mosseae L1) out of four tested depressed production of white clover shoots by 42% but enhanced that of leeks (Allium porrum) by 50%; the others were without effect. With dry peaty podzol and brown earth soils in pots, clover shoot production was highest with added P when a water holding capacity of 80% was maintained, but roots from the latter had only 2.6 compared to 68 nodules per plant from the former. Further work is required to explain poor nodulation in the brown earth soils.
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    Plant and soil 78 (1984), S. 445-448 
    ISSN: 1573-5036
    Keywords: Leucaena ; Rhizobium ; VA mycorrhiza
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Response ofLeucanea leucocephala to inoculation withGlomus fasciculatum and/or Rhizobium was studied in a phosphorus deficient unsterile soil.G. fasciculatum only inoculation improved nodulation by native rhizobia and Rhizobium only treatment improved colonization of roots by native mycorrhizal fungi. Dual inoculation with both the organisms improved nodulation, mycorrhizal colonization, dry weight, nitrogen and phosphorus content of the plants compared to single inoculation with either organism.
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  • 59
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    Plant and soil 78 (1984), S. 381-391 
    ISSN: 1573-5036
    Keywords: Aluminium ; Caloium ; Nodulation ; pH Phosphate ; Polymeric hydrolysis ; Rhizobium ; Rhizosphere ; Root elongation ; Root hairs ; Trifolium repens ; White clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effects of aluminium on theTrifolium repens var Huia-Rhizobium trifolii strain HP3 symbiosis were studied using an axenic solution-culture system. With, 10 μM phosphate, 50 μM aluminium reduced or inhibited root elongation at pH〈5.0, root hair formation at pH〈 5.0–5.5, and Rhizobium multiplication in the rhizosphere and nodule formation at pH〈6.0. In the absence of aluminium, root elongation and root hair formation were reduced at pH〈4.3, and Rhizobium multiplication and nodule formation were inhibited at pH〈5.0. Root hair formation was more sensitive to aluminium at pH〈5 than was root elongation. No effect of aluminium on Rhizobium multiplication and nodule formation at pH〈5 was detected because both were sensitive to pH alone. At pH 5.5 most of the aluminium changed immediately to a form which was susceptible to low-speed centrifugation, but which was detected by the aluminon method of analysis, and after 24 h a precipitate formed. the concentration of phosphate was reduced also, to approximately 1μM. Toxicity was overcome by either increasing the phosphate concentration from 10 to 50 μM, or by increasing the pH to 6.0 and the calcium, concentration to 1000μM.
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  • 60
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    Plant and soil 79 (1984), S. 101-121 
    ISSN: 1573-5036
    Keywords: Base saturation ; Bio-climatic zones ; Calcium ; Clay accumulation ; Copper ; C:N ratio ; EDTA ; Forest soils ; Greece ; Iron ; Magnesium ; Manganese ; Nitrogen ; Phosphate ; Potassium ; Soil classification ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soils derived from a number of different parent materials (lithologies) and developed along a climatic gradient, manifested by the altitudinal succession of natural vegetation zones (Mediterranean, sub-Mediterranean, Mountainous and Pseudoalpine), were sampled throughout mainland Greece. In soils derived from siliceous parent materials low in clay, acidity increase and percent base saturation decreases from the Mediterranean to the Pseudoalpine vegetation zones. Clay illuviation is found mainly in soils developed in the Mediterranean and the sub-Mediterranean zones. No such changes are apparent in clayey soils rich in bases. Organic matter content of the mineral portion of the soil profile increases by a factor of 2 with a decrease in mean annual air temperature of about 10°C. The pattern of change in clay and soil organic matter content with climate is in relatively good agreement with soil development trends in the area, when soil profiles are named according to the FAO-Unesco soil map of the world. Concentrations of Ca and Mg decrease and those of total N, total and extractable P, K, Fe, Mn and Zn increase from the Mediterranean to the Mountainous zone. Within the same zone, however, concentrations of N, Ca, K, Fe, Mn and Zn decrease, but those of Mg, total and extractable P increase with soil depth. The concentrations of most macro- and micronutrients in the humic horizon are several times higher than those in the mineral portion of the soil profile due to biological enrichment.
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    Plant and soil 80 (1984), S. 407-415 
    ISSN: 1573-5036
    Keywords: Cellulase ; Infection process ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The production of cellulase byRhizobium species was studied.Rhizobium trifolii cellulase was induced by a variety of polysaccharides, including celluloses and hemicelluloses. Cellobiose and myo-inositol also allowed enzyme expression but mannitol prevented it at concentrations higher than 0.25%. Both soluble and insoluble plant root substances moderately stimulated cellulase production byRhizobium trifolii. Most substances tested did not induce the production of cellulases by the “slow-growing, cowpea type” rhizobia strain CIAT 79. Effective inducers were carboxymethylcellulose, gluconate and myo-inositol. Cellulase production was very low under all conditions tested. In most cases the enzyme activity was loosely bound to the capsular material. The enzyme in fast-growers is an 1,4-β-D-glucan-4-glucanohydrolase (endo-glucanase EC 3.2.1.4) with specificity for high molecular weight polysaccharides. There was no correlation between infectiveness ofRhizobium trifolii strains and cellulase production. One strain, which lacks the nodulation plasmid, produced cellulase at the same rate as its parental infective strain.
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  • 62
    ISSN: 1573-5036
    Keywords: Acidity ; Aluminium ; Cowpea ; Nitrogen ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The interaction of pH (4 or 6), aluminium (0 or 16 ppm at pH 4) and N source (symbiotic or combined) on the growth and nutrient status of cowpea (Vigna unguiculata) was studied in a glasshouse experiment. Low pH significantly decreased the growth of the plants dependent on symbiotic nitrogen fixation but at pH 4 the addition of 16 ppm Al further depressed growth in both nitrogen regimes. Al-ions appear to exert their effect primarily on the root system, as shown by the reduction in total length and fresh weight. The symbiotic development of the plants was affected by low pH but more markedly by the Al treatment. Shoot nitrogen concentrations were reduced from ca. 2.6% at pH 6 to 1.8% and 0.9% at pH 4 without and with aluminium respectively. Calcium concentration was decreased by low pH and further by Al in both nitrogen regimes. In all Al-treated plants, the aluminium was mainly accumulated in the roots and was associated with an increase in their phosphorus concentration.
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  • 63
    ISSN: 1573-5036
    Keywords: Glycine max ; Inoculation ; IW/CPE ratio ; Nitrogen accumulation ; Nitrogen harvest ; Nitrogen re-distribution ; Nodulation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A field experiment was conducted on soybean (Glycine max (L.) Merrill) with a view to find out the effect of seed inoculation and scheduling of irrigation on nodulation, accumulation and re-distribution of nitrogen in plant tops and soil. The eight treatment combinations consists of two seed inoculations,viz. uninoculated and inoculated with rhizobium culture, and four irrigation schedules,viz. irrigation water to the cumulative pan evaporation (IW/CPE) ratio of 0.5, 0.7, 0.9 and a control (rainfed). Seed inoculation by, rhizobium culture increased the number, dry-weight and N content of nodules per plant. Inoculation of seeds also increased the N accumulation rate in plant top and it was 2.48 kg/ha/day during the flower-initiation to the pod-initiation stage (30–60 days interval). At harvest, 32.2, 47.8 and 26.2 kg N/ha was re-distributed from the stems, leaves and pods-wall of inoculated plants to the grains, respectively. A total of 186.5 kg N/ha was harvested and 64.7 kg N/ha, was accumulated in soil under the inoculated condition. Scheduling of irrigation at 0.7 IW/CPE proved better, than other irrigation schedules and helped in increasing the nodulation, nitrogen accumulation and grain yield. As compared to control, 8.4, 17.8 and 18.4 kg more of N/ha was redistributed from the stems, leaves and pods-wall respectively when the irrigations were scheduled at 0.7 IW/CPE ratio. Under this irrigation schedule the total N harvest was 200.1 kg/ha while the total N increased by 55.9 kg over that present in soil at the time of sowing.
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  • 64
    ISSN: 1573-5036
    Keywords: Clover ; Nitrogen fixation ; Rhizobium ; Root nodule ; Selection and plant breeding ; Symbiosis ; Trifolium pratense ; T. subterraneum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This paper reviews (i) basic studies on the genetics of symbiosis in red clover (a self-sterile species) and subterranean clover (cleistogamous) and (ii) work on selection and plant breeding to increase nitrogen fixation in these hosts. Symbiotic effectiveness in red clover is influenced by many major and minor genes. The highly effective phenotype is inherited in a complex manner associated with early nodulation and the formation of large amounts of persistent bacteroid-containing tissue. Lines bred to fix more nitrogen with one strain ofRhizobium trifolii do so with most but not all other strains examined. They also show slightly increased vigour when grown on nitrate. The highly effective response is correlated with abundant nodulation and an early flowering habit, the evidence from breeding studies indicating that this correlation is not absolute. Normally effective and highly effective nodules have the same specific nitrogenase activities. The expression of the highly effective response is relatively little affected by environmental factors (temperature, light intensity, day length, supplementary carbon-di-oxide). Inbreeding substantially degrades the symbiotic response. Heterosis is shown in crosses between cultivars of subterranean clover but otherwise selection to increase effectiveness in this host was unsuccessful. The relevance of these results (and their physiological aspects) for the improvement of grain legumes is discussed.
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    Plant and soil 82 (1984), S. 377-386 
    ISSN: 1573-5036
    Keywords: Centrosema ; Desmodium ; Inoculation ; Nitrogen yield ; Nodulation ; Oxisol ; Pueraria ; Rhizobium ; Soil cores ; Stylosanthes ; Tropical forage legumes ; Zornia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three phases of Rhizobium inoculation trials were carried out as part of a programme to select forage legume germplasm adapted to acid, infertile Oxisols of tropical America. Firstly, a range of tropical forage legumes were evaluated for their response to N fertilization or inoculation with strains previously shown to be effective in Leonard jars, using cores of undisturbed soil or in the field at Carimagua, Meta, Colombia. In pure legume stands onlyCentrosema macrocarpum andC. pubescens showed increases in N yield due to both inoculation and N fertilization;C. brasilianum responded only to N fertilization;Zornia latifolia, Z. brasiliensis andStylosanthes capitata responded to neither treatment. Trials in cores and in grass-legume mixtures showed responses ofDesmodium ovalifolium, Pueraria phaseoloides andS. capitata to N fertilization but not to inoculation. In the second phase of experiments strains were screened in soil cores with 16 ecotypes ofDesmodium, Centrosema, Stylosanthes andPueraria spp. Significant increases in N yield due to inoculation occurred with at least one strain in all the legumes exceptS. guianensis ‘tardio’, and in some trials withS. capitata. In the third phase of trials the most effective strains were tested in the field. Significant response ofP. phaseoloides andC. macrocarpum to inoculation at two sites and in the second year after establishment were shown. Further screening trials and field trials at different sites are needed in order to provide better recommendations for inoculation of grazing trials being set up in the region under study.
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  • 66
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    Plant and soil 80 (1984), S. 345-353 
    ISSN: 1573-5036
    Keywords: Ammonium ; Anaerobiosis ; Ion absorption ; Ion efflux ; Myrobalan plum rootstock ; Oxygen stress ; Potassium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Growth chamber experiments were conducted with ‘French’ prune (Prunus domestica L.) scions grafted on Myrobalan 29C (P. cerasifera Ehrh.) rootstocks grown in nutrient solution to characterize K and NH4 uptake before, during, and after anaerobiosis. Conditions of oxygen stress were imposed by removing the source of aeration and bubbling solutions with nitrogen gas. At solution oxygen concentrations less than 1%, K leaked from plant roots. After 18 h of anaerobic conditions, aeration was restored and K depletion from solution occurred within 2 h. Uptake of K the following day was similar to that before oxygen stress was imposed. Under similar conditions with solution oxygen concentrations less than 1%, both K and NH4 uptake were inhibited. Potassium leakage from roots was significantly greater than that of NH4. The presence of NH4 had no significant effect on K leakage from roots. Signs of wilting during oxygen stress appeared first on those trees that received NH4. Potassium uptake by rootstocks in the presence of NH4 was inhibited prior to and following anaerobiosis. However, the extent of NH4-induced inhibition of K uptake before anaerobiosis was similar to the K uptake inhibition after anaerobiosis.
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  • 67
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    Plant and soil 81 (1984), S. 85-92 
    ISSN: 1573-5036
    Keywords: Meloidogyne-Fusarium complex ; Nitrogen-form ; Potassium ; Cucumis melo ; Fusarium oxysporum f. sp.melonis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of various potassium concentration and of nitrate or ammonium was evaluated on non inoculated muskmelon plants and on plants inoculated with theFusarium wilt pathogen (F), the root-knot nematode (Meloidogyne javanica) (Nem), or a combination of both pathogens (F+Nem). Increasing potassium concentrations raised top fresh weights (TFW) in all four groups. Nitrate fertilized plants weighed more than plants receiving ammonium, independent of the K+ level in the medium. In the Nem+F infected plants TFW values were one and half to two times greater in those receiving the nitrate than in those receiving the ammonium. Number of nematodes in the roots were not affected by nutritional difference. In the ammonium fertilized F and Nem+F plants 30% more wilting was found than after nitrate application. Irrespective of the form of nitrogen that was applied accumulation of N, P and K was found in the roots of the Nem+F and/or Nem plants, while in the shoots Ca, Mg, Na and P accumulated and K was depleted.
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  • 68
    ISSN: 1573-5036
    Keywords: Calcium ; Eucalyptus saligna ; Eucalyptus wandoo ; Foliar nutrients ; Magnesium ; Nitrogen ; Phosphorus ; Potassium ; Rehabilitation ; Seasonal trends
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seasonal changes in the foliar concentration of macronutrients (N, P, K, Ca and Mg) in sapling trees ofEucalyptus saligna Sm. andE. wandoo Blakely growing in rehabilitated bauxite mined areas in the Darling Range of Western Australia are described. Foliar N concentration decreased with age of the fully expanded leaf tissue. Leaf N concentrations were also high when rates of litter decomposition were expected to be high during the period of early spring. The greatest foliar N difference between trees growing in good soil conditions and those from poorer soil conditions also occurred during this period. Levels of P in leaves were highest in young developing leaves but once the leaves reached full size, no seasonal trend in P concentration was observed. Foliar K was lower during the winter and probably related to the period of maximum leaching by precipitation. High foliar K during summer, however, could be related to the role of K in lowering cellular water potential. Leaf Ca was highest during early sping. Low mobility of cellular Ca during the cool portion of the year was indicated. Foliar Mg showed a weak pattern of decreasing concentration with leaf age. The best season for sampling for these broadleafed evergreen species to provide information on plant nutrient status appears to be in spring.
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  • 69
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    Plant and soil 82 (1984), S. 273-284 
    ISSN: 1573-5036
    Keywords: Legume ; Nitrogen fixation ; Rhizobium ; C and N economy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Studies of the C and N economy of a range of temperate and tropical legume/Rhizobium symbioses indicate considerable variation (up to three-fold) in the cost of N2 fixation. Comparisons between and within symbioses indicate that the proportion of net photosynthate utilized in nodule functioning varies almost ten-fold from as low as 3% to as high as 25%. Factors possibly responsible for variation in efficiency of C use in nodules and in the proportioning of translocated photosynthetic products to nodules are discussed.
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  • 70
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    Plant and soil 82 (1984), S. 329-335 
    ISSN: 1573-5036
    Keywords: Legume breeding ; Medicago sativa ; Nitrogen fixation ; Rhizobium ; Trifolium repens ; Vicia faba
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This paper examines evidence which quantifies the relative importance of legume and Rhizobium genotypes as determinants of phenotypic variation in symbiotic nitrogen fixation. It demonstrates potentially large and unpredictable effects of the Rhizobium genotype. The likely importance of such effects on crop yield is considered. The information is then used to assess ways in which legume breeding programmes may be altered to encompass the effects of genetic variation in Rhizobium.
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  • 71
    ISSN: 1573-5036
    Keywords: Centrosema ; Desmodium ; Inoculation ; Nitrogen yield ; Nodulation ; Oxisol Pueraria ; Rhizobium ; Soil cores ; Stylosanthes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three experiments were conducted in an acid infertile Oxisol from the Llanos Orientales of Colombia. It was shown that greater increases in nitrogen yield in the tops (N yield) due to N fertilization ofPueraria phaseoloides (CIAT germplasm accession no. 9900),Stylosanthes capitata no. 1019,Centrosema macrocarpum no. 5065 andDesmodium ovalifolium no. 350 occurred in undisturbed soil cores than in pots of disturbed soil. Inoculation significantly increased N yield ofC. macrocarpum in soil cores, but not in pots. In screening trials where a range of Rhizobium strains was used to inoculateD. ovalifolium, P. phaseoloides andC. macrocarpum grown in undisturbed soil cores, strains CIAT 2335, 2434 and 1780, respectively, caused the greatest increases in N yield. Inoculation caused greater increases of nodule numbers relative to the uninoculated control inC. macrocarpum than inD. ovalifolium orP. phaseoloides. When each legume was inoculated with the most effective strain by different methods, and grown in soil cores, it was found that granulated inoculant (0.5 g/seed) was more effective than seeds pelleted with 50 g inoculant/kg for the small-seededD. ovalifolium, but was similar in effectiveness to pelleted inoculant for the larger-seededP. phaseoloides. With the relatively large-seededC. macrocarpum, granulated inoculant was less effective than pelleted inoculant. No clear differences between different types of pellets were observed. The results show that undisturbed cores of acid infertile Oxisol can be used to screen for Rhizobium strains tolerant to these adverse soil conditions, although selected strains should then be subjected to further screening in the field, to determine whether they would make suitable commercial inoculants. It may not be possible to obtain maximum responses to inoculation by all legumes, unless the inoculation methods used here are improved.
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  • 72
    ISSN: 1573-5036
    Keywords: Acid rain ; Calcium ; Decomposition ; Leaching ; Magnesium ; Manganese ; Mobilisation ; Nitrogen ; Phosphorus ; Potassium ; Spruce litter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Dry matter and chemical changes in decomposing spruce needles were investigated after 16 and 38 weeks in laboratory lysimeters treated with distilled water or distilled water acidified to pH 3 or 2 with sulphuric acid. The water was added twice weekly in quantities equal to 100 or 200 mm month−1. The CO2 evolution and leaching of P, K, Mg, Mn, and Ca was followed together with pH measurements of the leachate. The loss of dry matter was approximately 25% during the first 16 weeks and approximately 37% after 38 weeks. At the first samling, 16 weeks, the amount of material decomposed was greater from the lysimeters given 100 mm month−1 of water. At this water quantity dilute sulphuric acid increased the decomposition. After 38 weeks sulphuric acid at pH 3 and 2 had decreased the decomposition at 200 mm month−1. However, the effects of acid application were small. The effect of treatment using acidified water on the content of monosaccharides was not consistent, whereas there was an indication of reduced decomposition of lignin when treated with 200 mm water month−1 at pH 3 and 2. Nitrogen was conserved in the lysimeters with small differences between the various treatments. The order of mobility of metal elements was K〉Mg〉Mn〉Ca. Increasing the quantity of water increased the leaching of K especially, whereas addition of dilute sulphuric acid increased the leaching of Mg, Mn and particularly Ca. During the first 16 weeks of the experiment, sulphuric acid reduced the leaching of P while later on this treatment increased the leaching. The pH of the leachate from the lysimeters treated with distilled water was initially 4.0–4.6 increasing to approximately 6.6 after 22 weeks. The pH of the decomposed needle material was 4.6 and approximately 5.2 after 16 and 38 weeks respectively. When treated with water at pH 3 the pH of the leachate was between 4 and 5, and the pH of the needles 4.2–5.1. Treatment with water at pH 2 gave a leachate with pH just above 2 and decreased the pH of the needles that had received 200 mm ‘rain’ month−1 to 2.9. The effect of the artificial acid rain appears to be more pronounced on the leaching of metal elements than on the biological activity and the dynamics of N and P. The treatments must be considered extreme when compared with the acidity of natural rain.
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  • 73
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    Plant and soil 57 (1980), S. 3-9 
    ISSN: 1573-5036
    Keywords: B-deficient ; B-toxic ; Corrections ; Growth ; Leaf ; Metabolism ; Molybdenum ; Nitrate ; NRase ; Potassium ; Root ; Sap ; Sugar ; Yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of deficient and toxic levels of boron on various aspects of nitrogen metabolism in sugar beet are studied. Plant analysis shows a nitrate ion accumulation, a decrease in the activity of the nitrate reductase enzyme and a lower molybdenum absorption. The effect of boron levels on the plant and root sugar concentration has also been studied.
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  • 74
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    Plant and soil 57 (1980), S. 467-470 
    ISSN: 1573-5036
    Keywords: Aluminum concentration ; Aluminum toxicity ; Calcium ; Magnesium ; Nutrient solution ; Phosphorus ; Potassium ; Triticale ; Rye ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of A1 on the growth and mineral composition of different cultivars of triticale (X Triticosecale, Wittmack), wheat (Triticum aestivum L.) and rye (Secale cereale L.) growing in 1/5 strength Steinberg solutions containing 0 or 6 ppm A1 were evaluated after 32 days. Aluminum increased the concentrations of P and K in the roots and K in the tops of most of the cultivars tested. A1 tolerant triticale retained a lower concentration of Mg in the roots and tops than the A1 sensitive triticale, when subjected to A1 stress. In addition, A1 treatments resulted in smaller increases in root P for the A1 tolerant triticale than for the A1 sensitive cultivars. The concentration of root Ca and P of the A1 tolerant wheat cultivars were significantly below that of the more sensitive plants. Aluminum tolerance in rye appeared to be associated with lower Ca and higher Mg concentrations in the tops. The accumulation of P and A1 in the roots was characteristic of sensitivity in triticale, wheat and rye.
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  • 75
    ISSN: 1573-5036
    Keywords: Distribution (shoots roots) ; Flowing solution culture ; H-ions ; Lolium perenne ; Potassium ; Sodium ; Uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The uptake of Na and K by perennial ryegrass from flowing solution culture with monitored concentrations of Na and K was followed in two experiments. In the first, when only 50 and 10 per cent of the K uptake by one set of plants, grown with K held constant at 2.5 μeq 1−1, was supplied to two other linked sets of plants and the balance supplied as Na, there was a rapid decrease in K, and an increase in Na, concentration in the shoots over a 20-day period. However, when compared with the plants grown in K in solution held constant, there was not a complete replacement of Na for K. In the second experiment the concentration of K in the culture solution was held constant at 2 μeq 1−1 and Na at 0, 5, 25, 50 and 100 μeq 1−1. Although uptake of Na increased with increasing concentration in solution the contents in the plants were low,i.e. less than 0.19 per cent and decreased with time. There was an increase in the yield of both shoots and roots with increasing Na in the solution; it was suggested that, during the early stages of growth there may have been an inadequate supply of K and that Na may have substituted for K in some of the non-specific roles of K in the plants. There was evidence in both experiments that a flux of H-ions was involved in the uptake of Na.
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  • 76
    ISSN: 1573-5036
    Keywords: Nutrient supply ; Nodule effectiveness ; Rhizobium competition ; Nodulation ; Lotus pedunculatus ; Lotus ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of nutrient supply on nodule formation and competition between Rhizobium strains for nodulation ofLotus pedunculatus was studied. Limiting plant growth by decreasing the supply of nutrients in an otherwise nitrogen-free medium, increased the size but decreased the number and the nitrogenase activity of nodules formed by a fast-growing strain of Lotus Rhizobium (NZP2037). In contrast decreasing nutrient supply caused only a small decline in the size, number and nitrogenase activity of nodules formed by a slow-growing strain (CC814s). Providing small quantities of NH4NO3 (50 to 250 μg N) to plants grown with a normal supply of other nutrients stimulated nodule development by both Rhizobium strains and increased the nitrogenase activity of the NZP2037 nodules. Differences in the level of effectiveness (nitrogen-fixing ability) of nodules formed by different Rhizobium strains on plants grown with a normal supply of nutrients were less apparent when the plants were grown with decreased nutrient supply or when the plants were supplied with low levels of inorganic N. Inter-strain competition for nodulation ofL. pedunculatus between the highly effective slow-growing strain CC814s and 7 other fast- and slow-growing strains, showed CC814s to form 42 to 100% of the nodules in all associations. The greater nodulating competitiveness of strain CC814s prevailed despite changes in the nutrient supply to the host plant. A tendency was observed for partially effective Lotus Rhizobium strains to become more competitive in nodule formation when plant growth was supplemented with low levels of inorganic nitrogen.
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  • 77
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    Plant and soil 60 (1981), S. 349-356 
    ISSN: 1573-5036
    Keywords: Competition ; Cowpea ; Ineffective ; Mutagenesis ; Mutants ; Nodulation ; Rhizobium ; Symbiotic and asymbiotic nitrogen-fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A strain of cowpea Rhizobium was mutagenised and two ineffective mutants, M1 and M2, and an effective mutant, M3, were isolated. M1 produced more, but smaller nodules than the wild-type; these nodules lacked leghaemoglobin. M2 and the parental strain had similar nodulation characteristics, both forming large pink nodules. Plants inoculated with M3, nodulated earlier, produced more nodules (58%), had increased dry weights (26%) and the excised roots expressed greater acetylene (C2H2) reducing activity (39%) than plants inoculated with the wild-type. When competing with an indigenous population of effective rhizobia for nodule sites, M3 produced a higher proportion of the nodules (70–80%) than the parental strain (40–53%). M3 and the parental strain exhibited comparable rates of asymbiotic C2H2 reduction when grown on a defined medium, whereas M1 and M2 were inactive. The symbiotic properties of the mutants were unchanged after their reisolation following plant passage.
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    Plant and soil 60 (1981), S. 445-450 
    ISSN: 1573-5036
    Keywords: Rhizobium ; Symbiosis ; Soil moisture stress ; Soybeans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In a pot culture experiment, the influence of soil moisture stress at different physiological stages of soybean, cv. Hark, on nodulation, symbiosis and nitrogen accumulation was studied. Moisture stress reduced leghemoglobin content of root nodules and nitrogen uptake by plants. It had no effect on number of bacteroids. Stress at mid bloom and rapid pod filling stages reduced yield and seed protein content. However, these parameters were not affected by stress at nodule initiation and early flowering stages, though, flower initiation and maturity of the plant were delayed. Moisture stress at any stage did not alter nitrogen status of roots.
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  • 79
    ISSN: 1573-5036
    Keywords: Gene centre ; Genetic variability ; Pisum sativum ecotype fulvum ; Rhizobium ; Symbiotic nitrogen fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Pisum sativum ecotype fulvum forms ineffective nodules with Rhizobium strains, isolated from effective nodules of the cultivated pea in Europe. Rhizobium strains isolated from nodules of fulvum peas in Israel are fully effective on this host plant, but in association with the cultivated pea they induce nodules of poor N2-fixing activity. The distribution of these fulvum-specific Rhizobium strains is restricted to regions where the fulvum pea occurs naturally. Rhizobium strains from other geographical regions induce mainly ineffective, or partially effective nodules on fulvum plants. A wide genetic variation, with regard to symbiotic response to a standard set of Rhizobium strains, was demonstrated in the fulvum plants collected in Israel. Based on variation in N2-fixation three groups of plants can be distinguished. These plants offer the possibility for the study of host-genetic control on symbiotic nitrogen fixation.
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  • 80
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    Plant and soil 80 (1984), S. 181-190 
    ISSN: 1573-5036
    Keywords: Adsorption ; Calcium ; CEC ; Cell wall ; Clover ; Exchange ; Magnesium ; Model ; Potassium ; pH ; Root ; Rye-grass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The ion exchange properties of clover and rye-grass root cell walls were studied quantitatively. Three sets of experiments were performed: adsorption of Ca, Mg and K ions versus pH, adsorption versus cation concentration and exchange isotherms Ca−Mg and Mg−K. A model has been developed. It allows the satisfactory prediction of results (except for pH curves) with the adjustment of a minimum of parameters. The total charge (RT), on its own, accounts for the difference between the ion exchange properties of the clover and rye grass cell walls. The selectivities observed on root material are very different from those observed on soil exchange complexes. The decreasing affinities of cell walls for cations follow the sequence: Ca〉Mg≫K for cell walls. These differences of selectivity are much larger than those usually observed for soil exchange complexes.
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  • 81
    ISSN: 1573-5036
    Keywords: Abscisic acid ; Gibberellin ; Polyethylene glycol ; Rhizobium ; Roots ; Nitrogenase ; Nodulation ; Soya ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of 2-day cycles of osmotically induced leaf moisture stress followed by partial recovery on the nodulation and nitrogenase activity of 2 soya cultivars was studied. Fourteen days after plant inoculation (mid-growth stage) the total leaf electrochemical water potential (ψwleaf) of control plants ranged from −0.8 to −1.9 bars, whereas the concentrations of osmoticum (polyethylene glycol 4000) induced ψwleaf values ranging from −1.4 (recovery value) to −3.1 bars (low stress), −1.8 to −4.4 bars (mild stress), and −2.2 to −6.2 bars (medium stress). The low stress treatment reduced nodule numbers and their specific activity in both cultivars, without affecting nodule size or the time required for nodule initiation. Nodule initiation was delayed in both cultivars by the mild and medium stress treatments, the former treatment reducing the number and size of the nodules and such nodules exhibited very low specific activity. The medium stress treatment prevented the further development of nodule initials, which remained inactive throughout the experiment. Such results imply an effect of water stress on the infection process and on nodule morphogenesis. The reduction in nodule numbers observed in water stressed plants was not associated with a reduced number of rhizobia in the rhizoplane nor due to an effect on root growth or root hair formation. At a stage prior to the formation of macroscopic nodule initials, the roots of plants under medium stress (ψwleaf=−5.5 bar)s) had a higher content of abscisic acid (ABA) (4-fold increase) and a lower content of gibberellin (GA)-like substances (21.4% reduction) as compared to control plants (ψwleaf=−1.0 bar). Although the medium stress treatment slightly increased the stomatal resistance of leaves, photosynthetic and transpiration rates were unaffected. Similar alterations of the hormononal balance occurred in the nodulated roots of plants subjected to naturally induced leaf moisture stress. Since the foliar application of ABA (1.92×10−5 M) to unstressed plants inhibited nodulation (45% reduction in nodule numbers), the increased endogenous content of thishormone in the roots of plants under leaf moisture stress may provide some physiological insight into the inhibitory effect of water stress on the nodulation process.
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    Plant and soil 80 (1984), S. 297-300 
    ISSN: 1573-5036
    Keywords: Nodulation ; Non-nodulating soybean ; Rhizobitoxine ; Rhizobium ; Soybean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A significant increase in nodulation of “non-nodulating” (rj1 rj1) soybeans was obtained by inoculating with very high numbers (approx. 1011 cells/pot) of certain rhizobia when compared with inoculation at a moderate dose (approx. 109 cells/pot). Nodulating ability of rhizobial strains was not correlated with their ability to produce a detectable level of chlorosis-inducing toxin in culture.
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  • 83
    ISSN: 1573-5036
    Keywords: Branch nutrients ; Calcium ; Eucalyptus saligna ; Eucalyptus wandoo ; Foliar nutrients ; Magnesium ; Nitrogen ; Phosphorus ; Potassium ; Rehabilitation ; Soil nutrients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The macronutrient variation within four 6 year oldEucalyptus saligna and four 5 year oldE. wandoo growing on rehabilitated bauxite pits was determined. Significant differences in mean nutrient concentrations were generally recorded between good soil condition sites and poor soil sites, between tree individuals, branch height, and plant organ type; but mean nutrient values were not different among canopy aspects. Fully expanded leaves of the current year provided the most uniform nutrient levels among the plant organs and showed major differences between sites with good soil nutrient conditions and those with poor conditions. Differences in foliar and branch levels of N, P, K, Ca and Mg, the variation between sites, canopy heights and plant organ types, and the use of foliar nutrient levels to indicate deficiencies are discussed.
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