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  • Triticum aestivum  (266)
  • Springer  (266)
  • Krefeld : Geologischer Dienst Nordhein-Westfalen
  • Wien : Geolog. Bundesanst.
  • 1990-1994  (266)
Collection
Publisher
  • Springer  (266)
  • Krefeld : Geologischer Dienst Nordhein-Westfalen
  • Wien : Geolog. Bundesanst.
Years
Year
  • 101
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    Sexual plant reproduction 5 (1992), S. 247-255 
    ISSN: 1432-2145
    Keywords: Wheat ; Triticum aestivum ; Pollen germinability ; Intracellular movement ; Actin cytoskeleton ; Effects of dehydration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The tricellular pollen of wheat germinates rapidly on a receptive stigma without the often protracted activation period characteristic of bicellular pollens. This is associated with a high level of hydration in the mature pollen and the absence of a dormancy period. Intracellular movement of organelles continues throughout development; in the mature pollen along pathways related both to the aperture site and the distribution of the amyloplasts in the vegetative cell. The movement pathways reflect the organisation of the actin cytoskeleton, elements of which are already focused upon the germination site at the time of dispersal, a disposition only achieved during rehydration and activation in bicellular pollens. Dehydration after dispersal rapidly arrests movement, disrupts the actin cytoskeleton and leads to loss of germinability. These effects are irreversible, again in contrast to the situation found in bicellular pollens such as those of the Liliaceae, species of which have been shown to be capable of withstanding several cycles of hydration and dehydration while still retaining some capacity for germination.
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  • 102
    ISSN: 1432-203X
    Keywords: Cell suspension ; Somatic embryogenesis ; Triticum aestivum ; Leymus angustus ; Plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Embryogenic cell suspension cultures were established from Triticum aestivum X Leymus angustus F1 hybrids, using compact nodular calli derived from inflorescence segments. Calli originating from leaf segments did not give rise to stable cell suspensions. Growth measurements of the cell suspensions revealed that they continued rapid growth up to 10 days after subculturing. Flow cytometric studies of the cell cycle over a 7 day culture period showed that the majority of cells were in G1 phase while the rest were either in S or G2. During the 7 days of culture, no significant differences in DNA distribution patterns were observed. The cells from suspension cultures produced somatic embryos when they were transferred to different solid media. The embryos germinated and gave rise to plantlets which were successfully rooted and transferred to soil.
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  • 103
    ISSN: 1432-203X
    Keywords: Triticum aestivum ; embryogenic suspension cultures ; protoplasts ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Highly regenerable callus cultures have been obtained from immature embryos of hexaploid wheat cv. Oderzo. Friable fast growing calli were induced at high frequency. Suspensions were initiated from the most friable callus lines: they became established in about two months. Suspensions consisted of cell aggregates of 30 to 1000 um in diameter. Upon plating on MS hormone-free medium, suspensions regenerated green plantlets, and their regenerative capability was maintained for at least 10 months. Protoplasts were isolated from 7–8 day old suspension cultures with a yield of 4–6×106 protoplasts/g fresh weight cells. Protoplast culture was either in liquid medium or in a bead-type system with agarose beads. First divisions were detected at day 5. At day 14 visible colonies were detected and the plating efficiency was evaluated between 2 and 8% over the initial number of protoplasts plated. Protoplast-derived calli were cultured in the presence of 1 mg/l IAA and 0.5 mg/l zeatin and were used for reinitiating new suspension cultures. Upon plating onto MS hormone-free medium, with or without the addition of 0.1 mg/l GA3, calliclones were induced to differentiate. Regeneration of complete plantlets, with shoot and roots took about two months. Plantlets were grown in sterile conditions until 12–15 cm height, and were subsequently transplanted in soil.
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  • 104
    ISSN: 1432-0983
    Keywords: Nuclear-cytoplasmic interactions ; Mitochondrial genome ; Chondriome variability ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Although the mitochondrial genomes of the Chinese Spring and Aquila varieties of wheat are normaly similar in organization, this is not so in tissue cultures initiated from their immature embryos where the mitochondrial genomes of both are rearranged and in different, characteristic, ways. However, the mitochondrial genomes of tissue cultures of reciprocal F1 crosses between these varieties were almost identical to one another, showing that nuclear genes control the rearrangement processes. These rearrangements are either due to the appearance of new structures or else result from changes in the relative amounts of subgenomic components. The severe reduction in the amount of certain molecular configurations in tissue cultures from reciprocal crosses is probably due to the presence of dominant information in the Aquila nuclear genome. Data obtained from tissue cultures initiated from F2 embryos of the cross Aquila x Chinese Spring suggest that at least two complementary genes are involved in this control. In contrast, the presence of new molecular arrangements appears to be under the control of a dominant allelic form of a Chinese Spring gene or genes. Thus, this study demonstrates that at least two sets of nuclear genes control the reorganization of the mitochondrial genome which occurs when tissue cultures are initiated from the immature embryos of wheat.
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  • 105
    ISSN: 1573-1561
    Keywords: Cover crops ; wheat ; Triticum aestivum ; soybean ; Glycine max ; soil extracts ; germination bioassays ; phenolic acids ; hydroxamic acids ; allelopathy ; slope analysis ; ivy-leaved morning glory ; Ipomoea hederacea ; crimson clover ; Trifolium incarnalum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The primary objective of this research was to determine if soil extracts could be used directly in bioassays for the detection of allelopathic activity. Here we describe: (1) a way to estimate levels of allelopathic compounds in soil; (2) how pH, solute potential, and/or ion content of extracts may modify the action of allelopathic compounds on germination and radicle and hypocotyl length of crimson clover (Trifolium incarnatum L.) and ivyleaved morning glory (Ipomoea hederacea L. Jacquin.); and (3) how biological activity of soil extracts may be determined. A water-autoclave extraction procedure was chosen over the immediate-water and 5-hr EDTA extraction procedures, because the autoclave procedure was effective in extracting solution and reversibly bound ferulic acid as well as phenolic acids from wheat debris. The resulting soil extracts were used directly in germination bioassays. A mixture of phenolic acids similar to that obtained from wheat-no-till soils did not affect germination of clover or morning glory and radicle and hypocotyl length of morning glory. The mixture did, however, reduce radicle and hypocotyl length of clover. Individual phenolic acids also did not inhibit germination, but did reduce radicle and hypocotyl length of both species. 6-MBOA (6-methoxy-2,3-benzoxazolinone), a conversion product of 2-o-glucosyl-7-methoxy-1,4-benzoxazin-3-one, a hydroxamic acid in living wheat plants, inhibited germination and radicle and hypocotyl length of clover and morning glory. 6-MBOA, however, was not detected in wheat debris, stubble, or soil extracts. Total phenolic acids (FC) in extracts were determined with Folin and Ciocalteu's phenol reagent. Levels of FC in wheat-conventionaltill soil extracts were not related to germination or radicle and hypocotyl length of either species. Levels of FC in wheat-no-till soil extracts were also not related to germination of clover or morning glory, but were inversely related to radicle and hypocotyl length of clover and morning glory. FC values, solute potential, and acidity of wheat-no-till soil extracts appeared to be independent (additive) in action on clover radicle and hypocotyl length. Radicle and hypocotyl length of clover was inversely related to increasing FC and solute potential and directly related to decreasing acidity. Biological activity of extracts was determined best from slopes of radicle and hypocotyl length obtained from bioassays of extract dilutions. Thus, data derived from the water-autoclave extraction procedure, FC analysis, and slope analysis for extract activity in conjunction with data on extract pH and solute potential can be used to estimate allelopathic activity of wheat-no-till soils
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  • 106
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    Plant molecular biology 18 (1992), S. 603-605 
    ISSN: 1573-5028
    Keywords: ARS-related DNA repeat ; DNA sequence ; Secale cereale ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 371 base pair segment (bordered by Hind III and Eco RI cutting sites) of wheat embryo nuclear DNA has been cloned and sequenced. It is AT-rich (68%), shares some sequence features with autonomously replicating sequence (ARS) elements, and occurs in approximately 7600 copies per haploid genome. When used as probe for blot hybridization to Hind III-digested wheat DNA, it gives an irregular series of hybridization bands. Essentially the same hybridization pattern was observed for rye DNA. It is concluded that this segment is distributed irregularly but, apparently, according to the same rule in both wheat and rye genomes.
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  • 107
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    Plant molecular biology 18 (1992), S. 1207-1208 
    ISSN: 1573-5028
    Keywords: Triticum aestivum ; nuclear tRNATyr gene ; DNA sequence ; transcription ; processing ; splicing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 108
    ISSN: 1573-5028
    Keywords: rRNA ; PCR ; ITS ; DNA sequence ; nucleotide ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 109
    ISSN: 1573-5028
    Keywords: mitochondrial DNA ; repeated sequences ; ribosomal RNA ; t-elements ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We report the sequence of a 7.2 kilobase pair DNA fragment containing a copy of the wheat mitochondrial gene (rrn26) that encodes the mitochondrial large-subunit ribosomal RNA (26S rRNA). The mature 26S rRNA was determined by direct RNA sequencing to be 3467 nucleotides long, and to share a 5′-terminal pentanucleotide (5′-AUCAU), thought to be important in post-transcriptional processing, with the wheat mitochondrial small-subunit (18S) rRNA. Two other prominent features of the sequence were noted. First, upstream of rrn26 are located two tandem copies of a 70 base pair element containing a putative mitochondrial promoter motif (TCGTATAAAAA). Second, downstream of rrn26 is a sequence element that, if transcribed, would produce and RNA with a secondary structure resembling that of tRNAs but differing sufficiently from the latter structure to preclude any transcript from functioning normally in translation. These upstream and downstream sequence elements may play a role in the expression of rrn26 in wheat mitochondria.
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  • 110
    ISSN: 1573-5028
    Keywords: mitochondria ; nad6 ; NADH-ubiquinone reductase ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A region of the mitochondrial (mt) DNA of wheat was studied because of its homology with other plant mtDNAs. Sequence analysis revealed an open reading frame encoding a polypeptide of 247 amino acids. Comparison of the sequence of the putative polypeptide with the protein sequence data of the Swiss-Prot library reveals homology with subunit 6 of the NADH-ubiquinone complex of mitochondria from Marchantia polymorpha, Podospora anserina, Chlamydomonas reinhardtii and of chloroplasts from M. polymorpha and Oryza sativa. No similarity was detected when compared with the subunit 6 of animal mitochondria, probably due to the rapid evolution of the sequence. A single 1.2 kb transcript appears in northern RNA blots. We found 15 edited sites of which only 13 give amino acid changes. This is the first report of a mt nad6 gene in higher plants.
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  • 111
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    Theoretical and applied genetics 84 (1992), S. 941-946 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Genetic differences ; Heat-shock proteins ; Heat-shock response ; DNA polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Heat-shock protein (HSP) gene expression in two wheat lines cv ‘Mustang’ (heat-tolerant) and cv ‘Sturdy’ (heat-susceptible) were analyzed to determine if wheat genotypes differing in heat tolerance also differ in in-vitro HSP synthesis (translatable HSP mRNAs) and steady-state levels of HSP mRNA. Several sets of mRNA were isolated from seedling leaf tissues which had been heat-stressed at 37 °C for various time intervals. These mRNAs were hybridized with HSP cDNA or genomic DNA probes (HSP17, 26, 70, 98, and ubiquitin). Protein profiles were compared using in-vitro translation and 2-D gels. The Northern slot-blot data from the heat-stress treatment provide evidence that the heat-tolerant cv ‘Mustang’ synthesized low molecular weight (LMW) HSP mRNA earlier during exposure to heat shock and at a higher level than did the heat-susceptible cv ‘Sturdy’. This was especially true for the chloroplast-localized HSP. The protein profiles shown by 2-D gel analysis revealed that there were not only quantitative differences of individual HSPs between the two wheat lines, but also some unique HSPs which were only found in the ‘Mustang’ HSP profiles. The high level of RFLP between the two wheat lines was revealed by Southern blot hybridization utilizing a HSP17 probe. These data provide a molecular basis for further genetic analysis of the role of HSP genes in thermal tolerance in wheat.
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  • 112
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    Theoretical and applied genetics 85 (1992), S. 73-78 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Intergeneric gene transfer ; Allosyndetic recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ‘Chinese Spring’ ph1b and ph2b mutants, as well as the nulli 5B tetra 5D stock were utilized in an attempt to effect homoeologous chromatin exchange between the ‘Indis’ chromosome translocation [derived from Thinopyrum distichum (Thunb.) Löve] and chromosome arm 7DL of common wheat. A homoeoallele of Lr19 and linked genes for yellow flour-pigmentation were utilized as markers. Seven selections with recombinations involving the foreign, translocated segment were recovered. Four of these had white endosperms and were leaf-rust resistant. The remaining lines were leaf-rust resistant and had levels of endosperm pigmentation intermediate to those of ‘Indis’ and ‘Chinese Spring’. The recombined translocation segments coding for white endosperm are no longer associated with chromosome 7D. The original translocated segment may, therefore, not be fully homoeologous to 7DL. The recombinants with white endosperm also lack the stem-rust resitance gene Sr25, but retained the segregation distorter locus, Sd-1. However, it seems as though an enhancer locus (or loci) of Sd-1 had been lost.
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  • 113
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    Theoretical and applied genetics 83 (1992), S. 301-304 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Genotype ; Environment interactions ; Cluster analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary One of the considerations of regional cultivar evaluation programs is to optimize the number of locations used for testing. Although optimization of numbers of locations using cluster analysis has been previously attempted, no objective comparison of methods has yet been made. A new clustering method that uses the pairwise contribution of locations to the cultivar x location mean square as the distance measure (LB) was compared to another method that employs diallel correlations as the distance measure (CL). Data from six spring wheat (Triticum aestivum L. em Thell) cultivars grown at 13 locations for five years were used in the initial cluster analysis. Another set of data, from a separate year, consisting of yields of the original 6 cultivars and a set of 12 independent cultivars was then used to check the validity of the original groupings and to compare the two methods. When the 6 original cultivars were considered, the LB technique was found to be superior to the CL. When the 12 independent cultivars were used, neither method was considered to be superior. Because of the lack of flexibility on the part of the LB method, neither technique could be deemed as fully adequate.
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  • 114
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    Theoretical and applied genetics 84 (1992), S. 1-5 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Secale cerale ; Alien gene expression ; Endosperm protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A series of hexaploid wheat lines containing zero, two or four doses of rye chromosome arm 1RS was used to investigate the response to changes in dosage by the rye genes when in a wheat background. The quantity of protein produced by the secalin protein genes contained on 1RS was directly related to the number of copies of 1RS present in the line. No response could be detected by representative wheat proteins suggesting that the increase in secalin protein observed was due to an increase in mRNA produced when four copies of the secalin gene was present. These results suggest that increasing the dosage of alien genes introgressed into wheat may be a useful tool for enhancing their expression. Mention of a trade name or proprietary product does not constitute a guarantee, warranty or recommendation of the product by the U.S. Department of Agriculture or the University of Missouri and does not imply its approval to the exclusion of other products that may be suitable.
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  • 115
    ISSN: 1432-2242
    Keywords: PCR ; 5S-ribosomal RNA ; Non-transcribed spacer ; Triticum aestivum ; Chromosome location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have used the polymerase chain reaction to analyse variation in the size of individual 5S-ribosomal gene spacer sequences. This reaction can be used to demonstrate inter- and intraspecific variation in spacer size, and combined with DNA sequencing it may thus be a valuable taxonomic tool. Two sets of nested polymerase chain reaction primers were designed to amplify the nontranscribed spacer DNA between repeated 5S-rRNA genes. These “universal” primers were used to generate fragments from the genomic DNA from several unrelated monocotyledonous plants. Ribosomal RNA spacer sequences generated in these experiments could also be used to locate 5S-rRNA gene clusters on specific chromosomes in hexaploid wheat (Triticum aestivum). Three distinct spacer sizes were observed after amplification. These were assigned locations on chromosomes by analysing amplification products of genomic DNA from nullisomic/tetrasomic and ditelosomic wheat stocks. “Large” 508-bp 5S repeats are located on the short arm of chromosome 5B and “short” 416-bp and 425-bp repeat unit variants are located on the short arms of chromosomes 1B and 1D, respectively. No other loci were detected. The spacer fragments were cloned, sequenced, and shown to be homologous to wheat 5S-rRNA spacers previously identified. Spacers of uniform size but with some sequence heterogeneity were shown to be located at each locus.
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  • 116
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    Theoretical and applied genetics 84 (1992), S. 259-265 
    ISSN: 1432-2242
    Keywords: Heading characters ; Geographical variation ; Adaptation strategy ; Genetic resource ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Heading time and its constituent traits, photoperiodic response, narrow-sense earliness and vernalization requirement, were surveyed for 158 wheat landraces. Wide varietal variation was observed in each character. Nearly half of the variation for each character was explained by a geographical difference in origin. Based on these data and the growing environments in each locality, we analyzed “adaptation strategy”, seen as the adjustment of heading time in terms of differences in the constituent traits, both individually and combined. The difference among localities indicated that wheat landraces had been selected for early heading as an adaptation strategy to water stress and/or high temperature in early summer. This change was caused by a reduction in photoperiodic response and narrow-sense earliness. The vernalization requirement was also reduced for adaptation to relatively mild winters. Adaptation strategy deduced from the variation within each locality was also different amongst localities. In the central region of wheat evolution, where wide variations existed in both photoperiodic response and narrow-sense earliness, the late-heading trait was achieved by either one of these traits individually or both of them combined. On the contrary, in the eastern and the western regions, wide variation in heading time was achieved by the unique combination of photoperiodic response and narrowsense earliness. A sampling strategy for wheat germ plasm is also discussed.
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  • 117
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    Theoretical and applied genetics 84 (1992), S. 528-534 
    ISSN: 1432-2242
    Keywords: Psathyrostachys juncea ; Triticum aestivum ; Isozyme markers ; Chromosome banding ; Intergeneric hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Psathyrostachys juncea (synonymous to Elymus junceus; 2n=2x=14, NN) has unique biotic and abiotic attributes that could contribute towards wheat improvement. The effectiveness of such an intergeneric hybridization program depends greatly on being able to establish diagnostic markers of the alien chromosomes. Isoelectric focusing (IEF) analyses of six enzyme systems have identified five biochemical markers — malate dehydrogenase (MDH), esterase (EST), shikimate dehydrogenase (SKDH), phosphoglucomutase (PGM), and β-amylase (β-AMY) — to be of positive diagnostic value; glucosephosphate isomerase (GPI) banding profiles were of no definite value in the background of Triticum aestivum cvs ‘Chinese Spring’ and ‘Seri-82’, the potential recipients of Ps. juncea chromosomes. The Giemsa C-banding karyotype distinctively separates the Ps. Juncea chromosomes from each other and from those of T. aestivum with little banding site polymorphisms prevalent among its accessions analyzed, indicating the usefulness of C-bands as cytological markers.
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  • 118
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Agropyron ; Intergeneric hybrids ; Backcross derivatives ; Chromosome pairing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intergeneric hybrids between Triticum aestivum cv ‘Chinese Spring’ and Agropyron cristatum 4x (2n= 5x=35, ABDPP genomes) with a high level of homoeologous meiotic pairing between the wheat chromosomes were backcrossed 3 times to wheat. Pollination of the F1 hybrid with ‘Chinese Spring’ resulted in 22 BC1 seeds with an average seed set of 1.52%. Five BC1 plants with 39–41 chromosomes were raised using embryo rescue techniques. Chromosome pairing in the BC1 was characterized by a high frequency of multivalent associations, but in spite of this there was no evidence of homoeologous pairing between chromosomes of wheat and those of Agropyron. All of the plants were self sterile. The embryo rescue technique was again essential to produce 39 BC2 plants with chromosome numbers ranging from 37 to 67. The phenomenon of meiotic non-reduction was also observed in the BC3 progenies. In this generation male and female fertility greatly increased, and meiotic pairing was fairly regular. Some monosomic (2n=43) and double monosomic (2n=44) lines were produced. Analysis of these progenies should permit the extraction of the seven possible wheat-Agropyron disomic addition lines including those with the added chromosomes carrying the genes involved in meiotic non-reduction and in suppression of Ph activity.
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  • 119
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    Plant systematics and evolution 183 (1992), S. 183-194 
    ISSN: 1615-6110
    Keywords: Poaceae ; Triticum aestivum ; 5S DNA sequences ; chromosomal5S Dna loci ; wheat evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A collection of 44 cloned 5S DNA units fromTriticum aestivum cv. ‘Chinese Spring’ were grouped into 12 sequence-types based on sequence similarity and the respective consensus sequences were then produced. The relationship between these 12 consensus sequences (T. aestivum S 1-S 8 andT. aestivum L 1-L 4), together with two clones sequenced byGerlach andDyer, and the 5S DNA consensus sequences from diploidTriticum spp. were then determined by numerical methods. Both phenetic and cladistic analyses were carried out. The following wheat 5S DNA sequences were found to group with respective sequences from diploidTriticum spp.:T. aestivum S 4, S 6 withT. tauschii S;T. aestivum S 3 withT. monococcum S andT. monococcum S-Rus 7;T. aestivum L 1 andT. aestivum L-G&D withT. speltoides L;T. aestivum L 2, L 3 withT. tauschii L;T. aestivum L 4 withT. monococcum L andT. monococcum L-Rus 12. The analyses suggested that 5 out of the 65S Dna loci present in wheat were identified at the sequence level. The locus that could not be identified in this analysis was the5S Dna-B 1 locus. A group ofT. aestivum sequences (T. aestivum S 1, S 7, S 8, S-G&D) were found to be distinct from the other 5S DNA sequences in the data base. The existence of the distinct group of 5S DNA sequences suggests that there is a gap in our current understanding of wheat evolution with respect to the5S Dna loci.
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  • 120
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    Molecular genetics and genomics 232 (1992), S. 215-220 
    ISSN: 1617-4623
    Keywords: 5S ribosomal genes ; Triticum aestivum ; Pulsed field gel electrophoresis ; Genetic fingerprinting ; Hypervariability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The long-range structure of 5S rRNA gene clusters has been investigated in wheat (Triticum aestivum L.) by means of pulsed field gel electrophoresis. Using aneuploid stocks, 5S rRNA gene clusters were assigned to sites on chromosomes 1B, 1D, 513 and 5D. Cluster sizes were evaluated and the copy number of 5S DNA repeats was estimated at 4700-5200 copies for the short repeating unit (410 bp) and about 3100 copies for the long repeat (500 bp) per haploid genome. A comparison of wheat cultivars revealed extremely high levels of polymorphism in the 5S rRNA gene clusters. With one restriction enzyme digest all varieties tested gave unique banding patterns and, on a per fragment basis, 21-fold more polymorphism was detected among cultivars for 5S DNA compared to standard restriction fragment length polymorphisms (RFLPs) detected with single copy clones. Experiments with aneuploid stocks suggest that the 5S rRNA gene clusters at several chromosomal sites contribute to this polymorphism. A number of previous reports have shown that wheat cultivars are not easily distinguished by isozymes or RFLPs. The high level of variation detected in 5S rRNA gene clusters therefore offers the possibility of a sensitive fingerprinting method for wheat. 5S DNA and other macro-satellite sequences may also serve as hypervariable Mendelian markers for genetic and breeding experiments in wheat.
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  • 121
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    Euphytica 60 (1992), S. 89-95 
    ISSN: 1573-5060
    Keywords: cuckoo effect ; gametocidal chromosome/gene ; preferential transmission ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A translocated chromosome segment, derived from Thinopyrum distichum, carries the leaf rust resistance allele Lr19d and a segregation distorter allele, Sd-1d. In translocation heterozygotes, male and female gametophytes lacking the translocation are aborted, the severity of the effect depending on the genotype of the hybrid. The selective abortion of the gametophytes with a normal chromosome 7D appears to be based on the absence of the translocated chromosome rather than the presence of the normal chromosome. The magnitude of the gametocidal response, elicited by Sd-1d, is under multigenic control. A number of chromosomes, the individual effects of which are generally small, may act to suppress or promote the response. Chromosome arms 2AL, 2BL, 5BL and 5DL of ‘Chinese Spring’ were found to reduce sensitivity to the presence of the gametocidal chromosome. Chromosome 3B of ‘Inia 66-1’ also reduce the gametocidal response while chromosome arm 6DS of ‘Chinese Spring’ may promote the effect
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  • 122
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; stress tolerance ; genetic variance ; genetic correlation ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat (Triticum aestivum L.) cultivars grown in the southern Great Plains of the U.S.A. are exposed to a wide range of moisture conditions due to large fluctuations in the amount and frequency of rainfall. Yield stability under those conditions is therefore a desirable trait for wheat breeders. Our primary objective was to quantify various genetic parameters for grain production in drought-stressed and irrigated environments. We also attempted to predict and measure yield responses when selection is practiced in either drought-stressed or irrigated environments, or both. Seventy F2-derived lines from the cross, TAM W-101/Sturdy, were evaluated at Goodwell, OK, under irrigated and naturally drought-stressed conditions in 1987 and 1988. Genetic variance and heritability estimates were higher in the irrigated environment than in the drought-stressed environment. The genetic correlation coefficient for yields in the two environments was 0.20±0.16, indicating that selection of widely adapted genotypes requires testing in both environments. Based on the genetic variance/covariance structure of this particular population, the linear index which maximized the combined expected gain in both environments was 0.66Y1 + 0.34Y2, in which Y1 and Y2 are yields in the irrigated and drought-stressed environments. This index is not expected to apply across all populations; rather, it further supports the hypothesis that testing in either environment alone (drought stressed or irrigated) may not be most effective for increasing either mean productivity or yield under drought stress.
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    Euphytica 62 (1992), S. 111-117 
    ISSN: 1573-5060
    Keywords: aluminium ; root growth ; screening ; tolerance ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Aluminium tolerance of 83 genotypes from Croatian and Yugoslav Triticum aestivum germplasm was evaluated in nutrient solutions having Al3+ activities of 0, 12.5 and 25 μM. Relative root length (25 μM Al3+/0 Al) of various genotypes ranged from 2 to 97% (from very sensitive to tolerant to Al). No genotype with Al tolerance close to that of very tolerant cultivar Atlas-66 was found. Soil, climatic, fertilization, and liming effects that wheat plants giving seeds for the nutrient solution Al-tolerance screening had been subjected to during their growth cycle did not influence the Al-tolerance ranking. Significant correlation was found between screening wheat for Al tolerance in nutrient solutions and in acid Pseudogley soil amended with five rates of limestone in a greenhouse experiment. Seed protein concentration was significantly related to the Al-tolerance ranking (r2 = 0.962). Such a significant correlation was not obtained in a case of rheological and other quality characteristics of seeds. Al-tolerant wheat genotypes identified in this study will be used in breeding for improved Al tolerance.
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  • 124
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    Euphytica 63 (1992), S. 103-113 
    ISSN: 1573-5060
    Keywords: durable resistance ; genetic diversity ; pathogenic variation ; pre-emptive breeding ; Puccinia spp. ; resistance breeding ; Triticum aestivum ; wheat rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Pre-emptive or anticipatory breeding for resistance is breeding for resistance to future pathotypes. It is assumed that these will be derivatives of currently frequent pathotypes that need to mutate with respect to single host resistance genes in order to attack widely-grown cultivars. Success in this approach depends on relevant knowledge of the pathogenicity phenotypes and host resistance genes that occur throughout the wheat-growing areas. Because durability of resistance cannot be assumed, resistance breeding strategies are usually supported with the maintenance of genetic diversity to provide buffering against extreme crop losses in the event of significant pathogenic changes.
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  • 125
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    Euphytica 62 (1992), S. 59-62 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; stomatal resistance ; chromosome 3A
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Leaf stomatal resistance, through transpiration and photosynthesis control, constitutes a major factor of productivity and adaptation in wheat. The aim of the investigations reported here was to identify chromosomal effects on the expression of the maximum stomatal resistance, determined under optimum conditions of irradiance and water supply. Leaf stomatal resistance was measured, on wheat grown in pots under natural and well-watered conditions, using a LI-COR LI-6200 portable photosynthesis system under a saturating light〉1400 μmol m-1 s-2. Reciprocal sets of chromosome substitution lines between two hard red winter wheat cultivars, Wichita and Cheyenne, were used to identify the chromosomes involved in the expression of this trait. The two parental cultivars were significantly different for the parameter investigated. Chromosome 3A appeared to be involved in the expression of the stomatal resistance value under optimum conditions. Taking into account the relationships previously established between this parameter and some agronomic traits, chromosome 3A might be involved directly in productivity-determining processes or in the adaptation to water conditions, in wheat.
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  • 126
    ISSN: 1573-5060
    Keywords: heading time ; narrow-sense earliness ; photoperiodic response ; Triticum aestivum ; vernalization requirement ; winter hardiness ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary For breeding early heading wheat cultivars with resistance to frost damage which are well adapted to dry areas of West Asia and North Africa, the relationships between winter hardiness, ear primordia development and heading traits, i.e. veernalization requirement, photoperiodic response and narrow-sense earliness, were assessed using a total of 30 genotypes of wheat (Triticum aestivum L.) grown in an experiment in Syria. The results of artificial freezing tests indicated that cultivars with good winter hardiness were to be found only in the winter wheat cultivars which required 50 or more days of vernalization treatment. These winter wheat cultivars did not initiate internode elongation without vernalization even at 95 days after planting. Thus their ear primordia were still underground and were protected from frost injury at this stage. Photoperiodic response and narrow-sense earliness were not associated with winter hardiness and earliness of internode elongation, but were related to the number of days to heading after planting. This indicated the possibility for breeding early heading cultivars with winter hardiness and tiller frost avoidance by combining high vernalization requirement, short narrow-sense earliness and neutral response to photoperiod.
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  • 127
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    Euphytica 63 (1992), S. 3-22 
    ISSN: 1573-5060
    Keywords: wheat ; Triticum aestivum ; yellow (stripe) rust ; Puccinia striiformis ; septoria ; Septoria tritici ; S. nodorum ; eyespot ; Pseudocercosporella herpotrichoides ; resistance genetics ; pathogen variation ; durable resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This introductory chapter contains some general comments about plant breeding and breeding for disease resistance. The use of disease resistant crop plants is an environmentally favourable method of controlling disease but the process of breeding for disease resistance is subject to several constraints. Among them is the variability of pathogens in relation to host resistance. Some parts of this variation can be resolved into gene-for-gene interactions, but the boundaries within which such interactions can be detected are not sharp. The discussion of this variation is illustrated by reference to some important diseases of wheat, especially yellow rust, septoria and eyespot. The objective of obtaining durable resistance is discussed and some contributions of new genetical and molecular techniques to breeding for resistance are considered. It is suggested that new technology will enhance breeding for disease resistance but that established techniques of plant breeding will remain relevant and important.
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  • 128
    ISSN: 1573-5036
    Keywords: 15N ; nitrogen harvest index ; nitrogen uptake efficiency ; soil water potential ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was conducted to investigate the influence of soil water potential, depth of N placement, timing, and cultivar on uptake of a small dose of labeled N applied after anthesis by wheat (Triticum aestivum L.) Understanding postanthesis N accumulation should allow better control of grain protein concentration through proper manipulation of inputs. Two hard, red spring-wheat cultivars were planted in early and late fall each yr of a 2-yr field experiment. Less than 1 kg N ha−1 as K 15NO3 was injected into the soil at two depths: shallow (0.05 to 0.08 m) and deep (0.15 to 0.18 m). In both years an irrigation was applied at anthesis, and injections of labeled N were timed 4, 12, and 20 days after anthesis (DAA). Soil water potential was estimated at the time of injection. Mean recovery of 15N in grain and straw was 57% of the 15N applied. Recovery did not differ between the high-protein (Yecora Rojo) and the low-protein (Anza or Yolo) cultivars. Mean recovery from deep placement was 60% versus only 54% from shallow placement (p 〈 0.01). Delaying the time of injection decreased mean recovery significantly from 58% at 4 DAA to 54% at 20 DAA. This decrease was most pronounced in the shallow placement, where soil drying was most severe. Regressions of recovery on soil water potential of individual cultivar x yr x planting x depth treatments were significant only under the driest conditions. Stepwise regression of 15N recovery on soil water potential and yield parameters using data from all treatments of both years resulted in an equation including soil water potential and N yield, with a multiple correlation coefficient of 0.64. The translocation of 15N to grain was higher (0.89) than the nitrogen harvest index (0.69), and showed a highly significant increase with increase in DAA. This experiment indicates that the N uptake capacity of wheat remains reasonably constant between 4 and 20 DAA unless soil drying is severe.
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  • 129
    ISSN: 1573-5036
    Keywords: relative addition rate ; relative growth rate ; traditional solution culture ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The relative addition rate (RAR) technique allows the nutritional control of plant relative growth rate (RGR) by the provision of nutrients at exponential supply rates. The technique, however, was developed with technologically sophisticated aeroponic systems. In this paper, we report on experiments used to adapt the RAR technique to a conventional solution culture system. A background concentration requirement of 36 μM nitrogen (N), with other nutrients supplied in proportion to N, was necessary to produce a constant RGR of Triticum aestivum L. (wheat) at a low RAR. Solution pH changes were reduced by increasing the percentage of NH4 in the nitrogen supply, but the plants exhibited dry weight reductions and symptoms of toxicity above 30% NH4. For wheat, a ratio of 25/75 NH4/NO3 was optimum for minimizing pH changes within the nontoxic range. A test of the effectiveness of the RAR technique using this background concentration and NH4/NO3 ratio showed that RGR increased with RAR with a linear slope of 0.55 and an intercept of 0.07 d-1. Although the relationship between growth rate and nutrient supply was less than the one-to-one dependence of RGR on RAR that has been obtained with more sophisticated apparatus, application of the RAR technique to a conventional solution culture system still affords considerable control of RGR and presents a simple method for growing plants at different levels of nutrient stress and at distinct RGRs.
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    Plant and soil 146 (1992), S. 55-59 
    ISSN: 1573-5036
    Keywords: acid soils ; low pH ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Shoot length (cm), shoot fresh weight (g/pot), root length (cm), and root fresh weight (g/pot) were measured on six cultivars of wheat (Triticum aestivum L. cv Saluda, C9733, Gore, Stacy, FL301, and FL302) grown at pH 6.0, 5.5, 5.0, 4.5, or 4.0 for 14 days in ‘white quartz flintshot’ sand. Plants were watered on alternate days with pH-adjusted buffer solutions. All measured plant parameters decreased as H+ concentration increased from pH 6.0 to 4.0. Decreased lengths of shoots and roots were similar among the cultivars as the pH decreased. This indicated a uniform response of wheat cultivars to excess H+ concentration in the soil solution; however, the decrease in shoot and root length was only about 50% as large as was previously reported for sorghum [Sorghum bicolor (L.) Moench.].
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  • 131
    ISSN: 1573-5036
    Keywords: aluminium ; electron microscope ; light microscope ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Root tips from aluminium (Al) tolerant (Waalt) and Al sensitive (Warigal) wheat (Triticum aestivum (L). Thell.) cultivars exposed to low concentrations of Al (10 μM) for 10, 24 and 72 hours were examined under the light and electron microscope. After fixing and embedding, longitudinal and transverse thin and ultrathin sections were cut. There was no evidence of Al damage to the root tips of the Al tolerant cultivar under both the light and electron microscope. For the Al sensitive cultivar, Al had no observable effect on the root tips 10 hours after Al addition when examined under the light microscope. When examined under an electron microscope, electron dense globular deposits were observed between the cell wall and cell membrane of the epidermal cells. There was not obvious damage to the cell cytoplasm. Two or 3 days after Al addition, light microscopy showed that the cells in the root tips had become swollen and extensively vacuolated. The tissues appeared disorganised and degenerate, particularly in the epidermis and outer cortical cells. The electron microscope also revealed a thickening of the cell wall. The cell wall was broken down, particularly in the epidermis in the region 4–6 mm from the root tip. The tissue in the meristematic area was largely intact.
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  • 132
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    Plant and soil 146 (1992), S. 241-250 
    ISSN: 1573-5036
    Keywords: Avena sativa ; genotypic differences ; grain zinc ; Hordeum vulgare ; Triticum aestivum ; zinc concentration ; zinc deficiency ; zinc uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Deficiencies of zinc are well known in all cereals and cereal-growing countries. From physiological evidence reported elsewhere, it would appear that a critical level for zinc is required in the soil before roots will either grow into it or function effectively; it is likely the requirement is frequently not met in deep sandy, infertile profiles widespread in southern Australia. Because fertilizing subsoils is impractical, this paper presents arguments for breeding cereal varieties with root systems better able to mobilise zinc from soil sources of low availability. Other agronomic arguments are presented in support of breeding for zinc efficiency. Significant genetic variation for this character is described for wheat, barley and oats. Linkage to other efficiency traits (e.g., manganese) is poor suggesting independent mechanisms and genetic control not linked to gross root system geometry. Zinc efficiency traits for sandy and clayey soils appear to be genetically different. Zinc-efficient genotypes absorb more zinc from deficient soils, produce more dry matter and more grain yield but do not necessarily have the highest zinc concentrations in tissue or grain. Although high grain zinc concentration also appears to be under genetic control, it is not tightly linked to agronomic zinc efficiency traits and may have to be selected for independently. High grain zinc is considered a desirable quality factor which not only contributes to the seedling vigour of the next generation but could increase the nutritional value of the grain in areas where a high dependence on grains for food may result in zinc deficiency in humans.
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  • 133
    ISSN: 1573-5060
    Keywords: Triticum turgidum ssp. dicoccoides ; wild emmer wheat ; Aegilops squarrosa ; synthethic hexaploid wheat ; breeding common wheat ; Triticum aestivum ; meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Novel amphiploids or synthetic hexaploid wheats, derived from eleven accessions of Triticum turgidum ssp. dicoccoides (wild emmer wheat, AABB, 2n=28) and eight accessions of Aegilops squarrosa (goat grass, DD, 2n=14) were studied. Most amphiploids were normal, vigorous and fertile. Among amphiploids a wide variation for morphological characteristics was observed. The glumes were found very tenacious and the rachis tough, or nearly so. First metaphase of meiosis proved to be quite regular; however, some aneuploid offspring occurred in successive generations. In F1 hybrids between T. aestivum (common wheat, AABBDD, 2n=42) ‘Chinese Spring’ and the synthetic hexaploids the rate of chromosome association was lower than in both parents, and significant differences were observed between amphiploids. Multivalents occurred in very low frequencies in these F1 hybrids. The rate of chromosome association in a hybrid between two synthetic hexaploids was found to be very low. The results are discussed in terms of genetic interactions and structural differences between the genomes of the respective species.
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    Genetic resources and crop evolution 39 (1992), S. 3-7 
    ISSN: 1573-5109
    Keywords: Triticum aestivum ; bread wheat ; Triticum durum ; durum wheat ; variation ; Rhodos
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Bread wheat plants contaminating two adjacent durum wheat fields near Tholos, Rhodos, could be classed in two groups. The origin of these two groups is unknown.
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  • 135
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    Nutrient cycling in agroecosystems 33 (1992), S. 161-175 
    ISSN: 1573-0867
    Keywords: Bicarbonate soil test for phosphorus ; Medicago polymorpha ; relative effectiveness ; residual value ; superphosphate ; Trifolium subterraneum ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of water supply on the response of subterranean clover (Trifolium subterraneum), annual medic (Medicago polymorpha) and wheat (Triticum aestivum) to levels of phosphorus (P) applied to the soil (soil P) was studied in four glasshouse experiments. P was applied as powdered superphosphate. In one experiment, the effect on plant yield of P concentration in the sown seed (seed P) was also studied. There were two water treatments: the soil was returned to field capacity, by watering to weight, either daily (adequate water, W1) or weekly (water stress, W2). In three experiments: (i) P concentration or content (P concentration × yield) in plant tissue was related to plant yield, and (ii) soil samples were collected before sowing to measure bicarbonate-extractable P (soil test P) which was related to subsequent plant yields. Compared with W1, water stress consistently reduced yields of dried tops and the maximum yield plateau for the relationship between yield and the level of P applied, by up to 25 to 60% in both cases. Compared with W1, the effectiveness of superphosphate for producing dried tops decreased for W2 by 11 to 45%, for both freshly-applied and incubated superphosphate. Consequently in the field, water supply, which varies with seasonal conditions, may effect plant yield responses to freshly — and previously — applied P fertilizer. Seed P increased yields, for W1, by 40% for low soil P and 20% for high soil P; corresponding values for W2 were 20 and 12%. Consequently proportional increases due to seed P were smaller for the water-stressed treatment. The relationship between yield and P concentration or content (internal efficiency of P use) differed for W1 and W2, so that the same P concentration or content in tissue was related to different yields. Estimating the P status of plants from tissue P values evidently depends on water supply, which in the field, differs in different years depending on seasonal conditions. The relationship between yield and soil test P differed for W1 and W2. Predicting yields from soil test P can only provide a guide, because plant yields depend on both P and water supply, which in the field may vary depending on seasonal conditions.
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  • 136
    ISSN: 1573-5044
    Keywords: plant regeneration ; protoplast ; somatic embryo ; suspension ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe the early formation of somatic embryos followed by plant regeneration from protoplasts isolated from an embryogenic wheat cell suspension, which was initiated from small granular (0.2 to 1 mm in size) embryogenic calli. These granular calli formed embryogenic cell suspensions within 20 days in liquid culture, and were selected gradually from young inflorescence-derived nodular embryogenic calli of the winter wheat cv. Kehong 1041. The division frequency of protoplasts was 11 to 16%, and the frequency of differentiation into plants was about 0.001% (number of plants formed divided by the total number of protoplasts plated). About 20% of somatic embryos present in the culture formed directly from protoplast-derived cells within 15 days of cultures.
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  • 137
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    Euphytica 61 (1992), S. 1-7 
    ISSN: 1573-5060
    Keywords: China ; crossability ; landrace ; rye ; Secale cereale ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The crossabilities of 177 landraces of wheat (Triticum aestivum L.) from Sichuan Basin and its adjacent mountain ranges with rye (Secale cereale L.) have been tested. 16 landraces possess a higher crossability than Chinese Spring, 34 landraces have a similar and 127 landraces have a lower crossability than Chinese Spring or are non-crossable with rye. Most landraces with high crossability occur in Qinling Mountain and Dabashan Mountain Ranges in north of Sichuan and the valleys of Minjiang River, Fujiang River and Jialinjiang River in Sichuan Basin.
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    Euphytica 61 (1992), S. 25-35 
    ISSN: 1573-5060
    Keywords: Mycosphaerella graminicola ; Septoria tritici ; septoria tritici blotch ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat cultivars of diverse genetical background and response to Septoria tritici were inoculated during 2 years in the field with single or mixtures of isolates. Significant reductions in pycnidial coverage were recorded for mixtures of 2 or 5 isolates relative to the virulent isolate ISR8036 under the moderate 1989/1990 epidemic. The interactions between cultivars and all possible combinations among ISR398A1, USR8036 and the 1:1. mixture of the 2 isolates were highly significant. Cultivars exposed to mixtures of isolates expressed differential response in pycnidial coverage compared to the single isolate response. The coverage in the mixtures was significantly less than that of the arithmetic mean between the two isolates. Under the severe 1990/1991 epidemic pycnidial coverage on cultivars inoculated with the mixture of the same 2 isolates did not differ statistically from that of ISR8036, yet, ISR398A1 differed from ISR8036 and the isolate mixture. Losses in 1000-kernel weight for 12 wheat cultivars which were repeated during the 2-trial-years were significantly lower in the isolate mixture relative to that of ISR8036. The suppression of symptoms in isolate mixture relative to the expected expression of the most virulent component may be indicative of differential aggressiveness of isolates regardless of their virulence. The phenomenon may affect screening and selection procedures in breeding for resistance.
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  • 139
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    Euphytica 60 (1992), S. 221-228 
    ISSN: 1573-5060
    Keywords: agronomic performance ; somaclonal variation ; tissue culture ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Seed progeny of tissue culture regenerants of a spring wheat (Triticum aestivum L. cv. HY320) was evaluated for key agronomic traits for three years under field conditions. Initially, 27 regenerant families were tested in hill plots. Among-family and within-family variation was generally highly significant (p 〈 0.01) and nonsignificant, respectively. The variation observed among regenerants on the basis of hill plot testing was not duplicated in subsequent four-row plot experiments. On average, regenerant families yielded 28 and 5% less than the control in dryland and irrigated tests, respectively. Low yielding regenerants tended to produce fewer, lighter kernels per spike. Higher grain protein levels among regenerants were associated with low yields (r=0.85). This study demonstrated that putative somaclonal variation arising from tissue culture failed to produce genotypes agronomically superior to the parental cultivar, HY 320.
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  • 140
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    Euphytica 61 (1992), S. 9-12 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; leaf rust ; Puccinia recondita ; adult plant resistance ; durable resistance ; substitution lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The leaf rust responses of wheat lines carrying the complementary genes Lr27 and Lr31 and the same genes in a Chinese Spring background which contains Lr34, indicate that Lr34 interacts with the complementary genes to give enhanced levels of field resistance to leaf rust. Lr34, particularly in combination with other genes, is considered to be an important gene for imparting a high degree of durable resistance to leaf rust. Its similarity to Sr2, an adult plant gene for resistance to stem rust and its association with adult plant resistances to stem and stripe rusts are discussed.
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    Euphytica 61 (1992), S. 123-130 
    ISSN: 1573-5060
    Keywords: rust resistance ; gene interaction ; Puccinia recondita ; Triticum aestivum ; leaf rust ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Leaf rust resistance gene Lr13 is present in many North American hard red spring wheat cultivars that have shown durable resistance to leaf rust. Fifteen pair-wise combinations of Lr13 and seedling leaf rust resistance genes were developed by intercrossing near isogenic Thatcher lines. In both seedling and adult plant tests, homozygous paired combinations of specific resistance genes with Lr13 had enhanced resistance relative to either parent to rust isolates that had intermediate avirulent infection types to the additional genes. In field tests, homozygous lines were more resistant than either parent if the additional leaf rust gene conditioned an effective level of resistance when present singly.
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  • 142
    ISSN: 1573-5060
    Keywords: black chaff ; Triticum aestivum ; Xanthomonas translucens ; inheritance ; resistance ; bacterial stripe ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The inheritance of resistance to bacterial leaf streak or black chaff of wheat was studied under field conditions, with an artificial epidemic of Xanthomonas campestris pv. undulosa. A complete series of crosses between five parents, differing in reaction to X. c. pv. undulosa, was generated. Disease was recorded at two different stages of growth. No evidence of cytoplasmic effect was found from the comparison between reciprocal F1 crosses. The study of the F3 generations attested that five genes were involved in resistance to bacterial leaf streak. Separate analyses carried out for the two scoring dates were mutually consistent: genotypes, number of genes, and their action and relative importance were verified. The genes differed in strength of expression of resistance. One of the two strongest genes, Bls1, is present in all three superior parents, Pavon 76, Mochis T88 and Angostura F88. Resistance was not complete, and proved to be stable over the season.
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  • 143
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    Euphytica 65 (1992), S. 1-8 
    ISSN: 1573-5060
    Keywords: adaptation ; barley ; bread wheat ; durum wheat ; triticale ; small grain cereals ; regression analysis ; stability parameters ; crop x environment interaction ; specific instabilities ; Triticum aestivum ; Triticum durum ; Hordeum vulgare, x Tritico secale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Yield data obtained from a comparative small grain cereals trial, grown for five consecutive growing seasons at a total of 23 environments in Cyprus, were subjected to regression analysis. Within each environment, yield trials consisted of a standard set of three cultivars or elite lines of barley, triticale, durum and bread wheat. The regression coefficient (b) of crop mean on the environmental index (I) and the mean square deviation from regression (sd2) were calculated for each crop. Each crop tended to have its own characteristic value of sd2 and its magnitude was an excellent indicator of specific crop-environment interaction. The causes of large sd2, for two of the four crops, were the susceptibilith of barley to lodging, when favourable conditions were encountered at high yielding environments, and triticale dependence on late season precipitation. Durum wheat and triticale had an average response to different yielding environments (b〉1.19) and both were significantly different from those of bread wheat (1.08) and barley (0.54). Hence, barley, bread and durum wheat are specifically adapted to low, average and high yielding Mediterranean environments, respectively. The cultivation of triticale at the expence of durum wheat is not feasible. Furthermore, interactions between crops and environments demonstrated by the regression parameters, should constitute the basis for decision making, regarding crop adaptation in a region. The average yield in all environments should not be considered as a proper criterion for adaptation. In this study, triticale had a similar mean grain yield (3,842 kg/ha) to that of bread wheat, but was significantly higher yielding than barley or durum wheat (5 and 7%, respectively).
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    Euphytica 65 (1992), S. 53-60 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Vrn genes ; heading date ; yield components ; vernalization ; growth habit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Vrn1, Vrn2 and Vrn3 genes have different values of effects on heading date and related yield components. The genetic background and environment do not affect the ranking of Vrn genotypes according to earliness within near-isogenic line sets; however, they do influence the level of differences between heading dates of particular genotypes and between effect values, respectively. The frequencies of defined Vrn genotypes in the global set of spring bread wheat cultivars are associated with grain weight per plant predicted on the basis of Vrn gene effects averaged over backgrounds and over environments. Peculiarities of backgrounds and environments alter the grain yield ranges of Vrn genotypes. For early photoperiod-insensitive wheats, planted in stress conditions at grain filling, the highest yield was predicted for double dominant Vrn genotypes with Vrn3. This gene is rarely used by the breeders in middle latitudes and its wider adoption is encouraged.
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    Euphytica 66 (1992), S. 187-195 
    ISSN: 1573-5060
    Keywords: dough mixing tolerance ; dough un-mixing time ; sticky dough problem ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The dough mixing tolerances of a number of non-1BL/1RS translocation wheat breeding lines and cultivars were measured using dough un-mixing time as the measure of mixing tolerance. For three different sets of wheats a large range in mixing tolerance was found. Wheats with short dough un-mixing times quickly broke down in the mixer to produce sticky doughs. Wheats with long dough un-mixing times possessed doughs which were more cohesive at optimum development, and therefore more resistant to breakdown by mechanical shear forces. Selection for dough mixing tolerance would ensure the breeding of wheat cultivars more suited for usage in high speed mechanical dough developers, and the modern food processing industry. Dough mixing tolerance, as measured by dough un-mixing time, was considerably influenced by flour protein content, and the environment. The incorporation of alien germplasms into wheat such as 1BL/1RS, and VPM-1, which produce sticky doughs, would be facilitated by selection for dough mixing tolerance, as measured by dough un-mixing time.
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  • 146
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    Plant and soil 147 (1992), S. 197-205 
    ISSN: 1573-5036
    Keywords: blade area ; grain yield ; leaf appearance rate ; phyllochron ; salt stress ; sand cultures, tillers ; Triticum aestivum ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Large initial seed size frequently confers distinct advantages on cereal crops in terms of seedling vigor, hardiness, improved stand establishment, and higher productivity. This study was conducted to determine if these advantages inherent in the plants grown from large seeds persist when the crop is subjected to salinity stress. Two hard red spring wheat cultivars, ‘Yecora Rojo’ and ‘Anza’ were grown in greenhouse sand cultures from seed of two size classes that differed in weight by a factor of 2. The cultures were irrigated four times daily with complete nutrient solutions to which NaCl and CaCl2 (2:1 molar ratio) were added to achieve osmotic potentials of −0.05. −0.55, and −0.70 MPa with electrical conductivities of 1.8, 12.8, and 15.8 dS m-1, respectively. In response to both salinity and small initial seed size, the following plant characteristics decreased: leaf appearance rate, blade area, tillers per plant, spikelets per spike and seeds per spike. Plants grown from large seeds out-yielded those from small seeds by 8, 37, and 27% for Yecora Rojo and by 15, 30, and 23% for Anza at osmotic potentials of −0.05, −0.55 and −0.70 MPa, respectively. Compared to the corresponding nonsaline controls, the yield of Yecora Rojo grown at −0.55 MPa was 51% for the plants from large seed and 35% from the small seeds. For Anza salinized at −0.55 MPa, these values were 49 and 40%, respectively. Exploitation of the benefits derived from large initial seed size may be a cost-effective management strategy for improving wheat productivity in salt-affected areas.
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  • 147
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    Plant and soil 145 (1992), S. 141-149 
    ISSN: 1573-5036
    Keywords: drought stress ; roots ; soil moisture ; transpiration ; Triticum aestivum ; water potential ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This investigation was performed to study the effect on plant water relations and growth when some of roots grow into dry soil. Common spring water (Triticum aestivum) plants were grown from seed in soil in 1.2 m long PVC (polyvinyl chloride) tubes. Some of the tubes had a PVC partition along their center so that plants developed a split root system (SPR). Part of the roots grew in fully irrigated soil on one side of the partition while the rest of the roots grew into a very dry (-4.1 MPa) soil on the other side of the partition. Split root plants were compared with plants grown from emergence on stored soil moisture (STOR) and with plants that were fully irrigated as needed (IRR). The experiment was duplicated over two temperature regimes (10°/20°C and 15°/25°C, night/day temperatures) in growth chambers. Data were collected on root dry matter distribution, soil moisture status, midday leaf water potential (LWP), leaf relative water content (RWC) and parameters of plant growth and yield. Some roots were found in the dry side of SPR already at 21 DAE (days after emergence) at a soil depth of 15 to 25 cm. Soil water potential around these roots was -0.7 to -1.0 MPa at midday, as compared with the initial value of -4.1 MPa. Therefore, water apparently flowed from the plant into the dry soil, probably during the night. Despite having most of their roots (around 2/3 of the total) in wet soil, SPR plants developed severe plant water stress, even in comparison with STOR plants. Already at 21 DAE, SPR plants had a LWP of -1.5 to -2.0 MPa, while IRR and STOR had a LWP of -0.5 MPa or higher. As a consequence of their greater plant water stress, SPR as compared with IRR plants were lower in tiller number, ear number, shoot dry matter, root dry matter, total biomass, plant height and grain yield and had more epicuticular wax on their leaves. It was concluded that the exposure of a relatively small part of a plant root system to a dry soil may result in a plant-to-soil water potential gradient which may cause severe plant water stress, leading to reduced plant growth and yield.
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  • 148
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Triticum durum ; bread wheat ; durum wheat Secale cereale ; rye ; gene expression ; alien introduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Expression of 17 rye traits in 24 bread wheat x rye and 8 durum wheat x rye crosses was studied, using a self-compatible, homozygous, dwarf rye. Rye showed epistasis for hairiness on the peduncle in all the crosses of Triticum aestivum and T. durum wheats with rye. Dark greenness of leaves of rye was expressed in all the durum wheat x rye and in some of the bread wheat x rye crosses. Similarly, absence of auricle pubescence, a rye trait, was expressed in most of the durum wheat x rye crosses but not in the bread wheat x rye crosses, indicating the presence of inhibitors for these traits frequently on the D genome and rarely on the A and/or B genome of wheat. Most of the wide hybrids resembled rye fully or partially for intense waxy bloom on the leaf-sheath and for the absence of basal underdeveloped spikelets. Similarly, most of the amphihaploids resembled rye for the anthocyanin in the coleoptile, stem and node. The presence of some inhibitors on A and/or B genome of wheat was indicated in some of the wheat genotypes for the expression of rye traits viz. intense waxy bloom, anthocyanin in node and absence of basal underdeveloped spikelets. Enhancement in the level of expression of the intensity and length of bristles on the mid-rib of the glume of the hybrids might be due to wheat-rye interaction. Less number of florets/spikelet as in rye showed variable expression in different wheat backgrounds. Some other rye traits like absence of auricles, terminal spikelet and glume-awn were not expressed in the wheat background. The expression of some of the rye genes might have been influenced by their interaction with Triticum cytoplasm and/or the environment.
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  • 149
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    Euphytica 63 (1992), S. 265-270 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; somatic embryogenesis ; wheat tissue culture genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A spring wheat genotype which produces somatic embryos in vitro, after short and long-term culture, was tested for its ability to sexually transmit this embryogenic trait. Reciprocal crosses were performed between a embryogenic line and a nonembryogenic variety. Immature embryos were cultured on Murashige and Skoog medium plus 2 mg/l 2,4-dichlorophenoxyacetic acid, gelled with 5.5 g/l agarose. Somatic embryogenesis was not expressed in the F1's. In contrast, from several hundred immature embryos of the F2 generation of one cross, 10.7% and 1.6% expressed somatic embryogenesis in short and long-term cultures respectively. These percentages of embryogenic: non-embryogenic fits a model of a few complementary genes. The embryogenic capacity of the F2 genotypes depends on the presence of recessive alleles at these gene loci. The long-term wheat somatic embryogenesis capacity requires a more complex mechanism than the short-term one.
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  • 150
    ISSN: 1573-5060
    Keywords: tissue culture ; somaclonal variation ; plant breeding ; Triticum aestivum ; mutation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plants were regenerated from immature embryo cultures of 35 winter wheat genotypes. A total of 7142 R2 spike lines from 1593 R1 plants were assessed in the field for somaclonal variants of morphological traits in 1985/86, 1986/87 and 1987/88. Selected variants were studied for their possible genetic basis. Populations of R1 plants were highly variable due mainly to the physiological disturbances resulting from the in vitro processes. Overall somaclonal variation frequencies were 14.2% on the R1 plant basis and 5.3% on the R2 spike basis. Spectra of the variants were similar in the different R2 populations with predominant variants being altered negatively in plant height, maturity, awnedness, and spike and plant types. Over 90% of the variants were observed in some spike progenies of individual regenerants, while the others appeared in all spike progenies of the regenerants and in progenies of different regenerants derived from the same explant embryos. Both uniform R2 variant families and spike lines were found in addition to the segregating variants, which constituted the majority. On average, in a variant family and line, 18 and 14% of their component lines and plants varied, respectively. Inheritability was demonstrated for the variations in both segregated and uniform variant families and spike lines. Of 134 variant selections tested, about 70% was classified inhernable. Both recessive and dominant gene mutations at one, two or three loci were evident in some of the variants as suggested by segregation data.
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  • 151
    ISSN: 1573-5060
    Keywords: winter wheat ; Triticum aestivum ; Russian wheat aphid ; Diuraphis noxia ; coldhardiness ; fructan ; osmotic potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of an autumn Russian wheat aphid, Diuraphis noxia (Mordvilko), infestation on winter wheat, Triticum aestivum L., was investigated using osmotic potential, fructan content and field survival measurements to estimate coldhardiness. An average infestation of 147 aphids per plant, under simulated hardening conditions, increased the osmotic potential of ‘Froid’ and ‘Brawny’ (more or less coldhardy varieties, respectively) seedlings by 0.236 and 0.345 MPa, respectively. A natural field infestation averaging 9.7 aphids per plant reduced fructan contents of Froid and Brawny seedlings by 22.60 and 59.10 g kg-1 dry wt., respectively. Similar trends occurred after a natural freeze period. Field survival and yield results indicate that the autumn infestation reduced the winter survivability of Brawny by 54% and reduced grain yields by 1217 kg ha-1; survival and yield of infested Froid were not significantly affected. PI 372129, a D. noxia resistant winter wheat genotype was not affected by an infestation in regard to these parameters. These studies suggest that coldhardiness in Froid and Brawny was reduced by D. noxia though only the less hardy Brawny had a significantly reduced winter survivability and grain yield. Therefore, the effects of an autumn D. noxia infestation on some winter wheat genotypes may interact with a winter climate and increase the potential for winterkill.
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  • 152
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    Euphytica 64 (1992), S. 11-20 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; breadmaking quality ; high molecular weight ; glutenin subunits ; electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The endosperm storage proteins of 205 Chinese bread wheat cultivars and advanced lines were fractionated by SDS-PAGE to determine their high molecular weight (HMW) glutenin subunit composition. Seventeen alleles were identified: three at Glu-A1, eight at Glu-B1, and six at Glu-D1. The most common alleles were Null, 1, 7+8, 7+9, and 2+12. The results indicate that wheats from different regions differ in their frequencies of HMW glutenin subunits, however, none of the subunits could be related to specific environments. The glutenin quality scores of Chinese wheats ranged from 3 to 10, with an average of 6.7. Increasing quality scores have implications in improving steam-bread making quality for Chinese consumers. On the basis of HMW glutenin subunit composition, Chinese wheats are close to European wheats, especially Italian wheats because several Italian introductions are widely distributed in the pedigrees of Chinese wheat.
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  • 153
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; starch-granule bound (Wx) protein ; amylose content ; Japanese noodles ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The starch-granule bound proteins of common wheat (Triticum aestivum L.) and six related species were examined by SDS-polyacrylamide gel electrophoresis. They showed almost the same molecular weight, 61 kD. The amount of the starch-granule bound protein varied among Japanese wheat cultivars although the variation was not as wide as that for rice. The amount of this protein of Kanto 107 and Kanto 79 was about 40% of that of Norin 98. In addition, wheats with lower starch-granule bound protein also possessed lower amylose content.
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  • 154
    ISSN: 1573-5060
    Keywords: doubled haploid ; genetic marker ; wheat ; wheat x maize crosses ; Triticum aestivum ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat doubled haploid (DH) lines were produced from the F1 hybrid, Fukudo-komugi x Oligo Culm, through intergeneric crosses between wheat and maize. F2 plants and 203 DH lines were analyzed for the segregation of the eight genetic markers, namely, grain proteins, grain esterases, GA-insensitivity and glume traits. The segregation in the F2 plants fitted to the expected ratios. No deviation was observed among the DH lines, either, except for the glume pubescence. The result indicates the absence of correlation between the markers investigated and the efficiency of embryo formation in the DH lines.
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  • 155
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    Euphytica 66 (1992), S. 111-116 
    ISSN: 1573-5060
    Keywords: Al tolerance ; Croatia ; genetic variation ; gene pool ; root growth ; Triticum aestivum ; wheat ; Yugoslavia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Aluminium tolerance of 90 genotypes of Triticum aestivum L. germplasm from the breeding programmes of eight Croatian and Yugoslav institutions was evaluated in nutrient solutions having Al3+ activities of 0, 12.5 and 25μM. Overall distribution of Al tolerance of wheat genotypes was skewed toward lower tolerance rankings. Average Al tolerance differed among gene pools created at different breeding institutions. Genotypes tolerant enough to be useful in the breeding programmes aimed at selecting cultivars with improved Al tolerance are identified in germplasm from four institutions. No correlation was found between chemical characteristics of soils used over the years by breeding institutions for their field trials and the Al-tolerance ranking of the corresponding germplasm material.
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  • 156
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    European journal of plant pathology 98 (1992), S. 369-376 
    ISSN: 1573-8469
    Keywords: Triticum aestivum ; Mycosphaerella graminicola ; Phaeosphaeria nodorum ; Stagonospora nodorum ; epidemiology ; weather
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Data of the annual surveys of circa 100 commerical winter wheat fields were compiled to describe epidemics ofSeptoria spp. in the Netherlands during 1974–1986. In May, during the first node stage,S. tritici was dominant whileS. nodorum was virtually absent. In July, during ripening,S. tritici on average dominated overS. nodorum, though in the most continental districts of the countryS. nodorum predominated. In May between 1974 and 1984, on average 56% of the fields showed leaf infections bySeptoria spp., while in July between 1975 and 1986, on average 83% of the fields showed leaf infections. Prevalence ofSeptoria spp. has increased during the surveys. Annual intensity ofSeptoria spp. in winter wheat crops was positively correlated with precipitation and negatively with average monthly sunshine duration during the harvest-month August of the previous growing season. The correlation with sunshine during August could indicate that ascospores play a major role in subsequent epidemics; but whether it is a causal relation remains to be answered.
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  • 157
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    European journal of plant pathology 98 (1992), S. 301-312 
    ISSN: 1573-8469
    Keywords: Triticum aestivum ; Erysiphe graminis ; Blumeria graminis ; Puccinia striiformis ; P. recondita ; P. graminis ; disease surveys ; epidemiology ; weather ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Results of annual surveys of winter wheat fields from 1974 to 1986 were compiled to describe epidemics of powdery mildew and rusts in relation to weather and cultivar resistance. An average of 29 and 70% of fields were infected by powdery mildew in May and July, respectively. Mildew prevalence in May was positively correlated with average temperature in October and with average temperature over the months December, January, February and March. In addition, it was correlated negatively with the average grade of mildew resistance of the cultivars sown each year. Prevalence of mildew in July did not show consistent correlations with weather characteristics nor with mildew prevalence in May. Yellow rust was usually not detected in May and on average 18% of the fields was infected in July. The occurrence of yellow rust decreased after 1977, when the farmers adopted cultivars resistant or moderately resistant to yellow rust. Brown rust was usually not detected in May, while in July on average 48% of the fields was infected. Brown rust intensity in July was high in years with a high March temperature and high precipitation during April and May. Black rust was rare in the Netherlands, with 3 and 1% of the fields infected in July 1977 and 1981, respectively.
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    Entomologia experimentalis et applicata 58 (1991), S. 69-74 
    ISSN: 1570-7458
    Keywords: Rhopalosiphum padi ; antixenosis ; plant resistance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Die Antixenose gegenüberRhopalosiphum padi (L.) in den drei Weizensorten Timmo, Moghan 2 und Ommid, von denen die zwei letzteren Sorten aus dem Iran stammen, wurde unter Verwendung des systemischen Insektizids Pirimicarb gemessen. Jeweils zwei Pflanzen aus verschiedenen Sorten wurden als Paar verwendet (in allen Kombinationen der drei Sorten), pro Paar ein Topf mit Pirimicarb gegossen, und zwanzig Blattlaüse über Blattflächen von beiden Sorten gekäfigt. Ähnliche Versuche wurden auch ohne Insektizid ausgeführt. Beide Methoden zeigten deutliche Antixenose von Moghan 2 und Ommid in Vergleich mit Timmo. Die Insektizidmethode zeigte auch eine Bevorzugung von Moghan 2 gegenüber Ommid, ein Resultat, das mit der schon bekannten relativen Antibiose dieser zwei Sorten übereinstimmte, während sich ohne Insektizid kein solcher Unterschied erwies. Die statistische Analyse solcher Versuche mit einem Insektizid, die eine Modifikation der Berechnung des erwarteten Werts mit dem χ2 Test enthält, ist beschrieben.
    Notes: Abstract Antixenosis in three wheat varieties (Timmo, Moghan 2 and Ommid) toRhopalosiphum padi (L.) was measured by pairing plants of any two varieties with one plant of each pair treated with a soil drench of the systemic insecticide pirimicarb. Aphids were then allowed access to equivalent leaf areas from the two plants in a leaf cage. The results were compared with similar choice experiments without insecticide. By either technique, the two Iranian varieties (Moghan 2 and Ommid) showed antixenosis in comparison with Timmo. The statistical analysis of the data, using a heterogeneity χ2 test, is described.
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    Entomologia experimentalis et applicata 59 (1991), S. 79-85 
    ISSN: 1570-7458
    Keywords: Wheat ; Triticum aestivum ; cereals ; Rhopalosiphum padi ; Aphididae ; barley yellow dwarf virus ; hydroxamic acids ; DIMBOA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 2,4-Dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), a hydroxamic acid (Hx) occurring in wheat, was shown to deter feeding by the aphid Rhopalosiphum padi (L.), and to reduce BYDV transmission to the plant. Dual choice tests with wheat leaves showed the preferential settlement of aphids on leaves with lower levels of DIMBOA. Electric monitoring of aphid feeding behaviour showed that in seedlings with higher DIMBOA levels fewer aphids reached the phloem and they needed longer times to contact a phloem vessel than in those with lower levels. When aphids carrying BYDV were allowed to feed on wheat cultivars with different DIMBOA levels, fewer plants were infected with BYDV in the higher DIMBOA cultivars than in the lower ones. Preliminary field experiments showed a tendency for wheat cultivars with higher Hx levels to be more tolerant to infection by BYDV than lower Hx level ones.
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  • 160
    ISSN: 1432-0983
    Keywords: Light regulation ; psbN ; Triticum aestivum ; Etioplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleotide sequence of a region of wheat chloroplast DNA containing the psbB gene for the 47 kDa chlorophyll a-binding protein of photosystem II has been determined. The gene encodes a polypeptide of 508 amino acid residues which is predicted to contain six hydrophobic membrane-spanning regions. The psbB gene is located 562 bp upstream of the psbH gene for the 10 kDa phosphoprotein of photosystem II. A small open reading frame of 38 codons is located between psbB and psbH, and on the opposite strand the psbN gene, encoding a photosystem II polypeptide of 43 amino acid residues, is located between orf38 and psbH. S1 nuclease mapping indicated that the 5′ ends of transcripts were located 371 and 183 bp upstream of the psbB translation initiation codon. Predominant transcripts of 2.1 kb and 1.8 kb for psbB and 0.4 kb for psbH were present in RNA isolated from etiolated and greening wheat seedlings. Immunodecoration of Western blots indicated that the 47 kDa polypeptide was absent, or present in very low amounts, in dark-grown tissue and accumulated on greening, whereas the 10 kDa polypeptide was present in similar amounts in both dark-grown and greening seedlings. The 10 kDa polypeptide was phosphorylated in vitro by incubating wheat etioplast membranes with [γ3 2P] ATP.
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  • 161
    ISSN: 1432-0983
    Keywords: Mitochondrion ; Transcription ; Guanylyltransferase ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To identify transcription initiation sites in wheat mitochondria, the nascent 5′-ends of transcripts were specifically labeled by incubation of wheat mitochondrial RNA with [α-32P]GTP in the presence of the enzyme guanylyltransferase. After separation of the resulting capped transcripts by electrophoresis in polyacrylamide gels, individual RNAs were recovered and directly sequenced. Four RNA sequences obtained in this way were localized upstream of the protein-coding genes atpA, coxII, coxIII and orf25. Comparison of mRNA and gene sequences allowed precise positioning of transcription initiation sites for these four genes. Sequence similarities immediately upstream of these sites define a conserved motif that we suggest as a candidate regulatory element in wheat mtDNA. The relationship between this motif and putative mitochondrial promoters in other plant species is discussed.
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  • 162
    ISSN: 1573-5028
    Keywords: chloroplast genes ; cytochrome b6 ; introns ; Triticum aestivum
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    Plant molecular biology 16 (1991), S. 1099-1101 
    ISSN: 1573-5028
    Keywords: wheat ; Triticum aestivum ; cDNA clone ; waxy protein
    Source: Springer Online Journal Archives 1860-2000
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  • 164
    ISSN: 1432-2242
    Keywords: Preferential transmission ; Triticum aestivum ; Aegilops sharonensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The transmission of chromosome 4S l from Aegilops sharonensis was observed in a range of wheat genetic backgrounds. Chromosome 4S l was transmitted at a very high frequency (at least 97.8%) in all crosses. The genetic background appears to only have a small effect on transmission. The frequency of transmission of chromosome 4S l was the same in each genetic background through both the male and female gametes.
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    Theoretical and applied genetics 81 (1991), S. 576-580 
    ISSN: 1432-2242
    Keywords: Pollen culture ; Pollen maturation ; Pollination ; Triticum aestivum ; Gliadins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Immature pollen of two varieties of Triticum aestivum, at the stage right after the first pollen mitosis, was isolated from individual anthers and cultured in microcultures of microliter droplets. In a specifically designed medium, some of the pollen grains developed to maturity. These were applied to excised stigmas on agar, where they produced pollen tubes. Application to flowers in vivo led to seed set. Pollen was matured in vitro from a variety that produced a different protein banding pattern on SDS-PAGE as compared to the variety that was pollinated. The protein banding in the produced seeds showed the hybrid pattern, demonstrating that the seeds were not produced by self-pollination in this in-breeding species but by pollination with the in-vitro-matured pollen.
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    Theoretical and applied genetics 82 (1991), S. 615-620 
    ISSN: 1432-2242
    Keywords: HSP-70 ; BiP/GRP-78 ; Wheat ; Triticum aestivum ; Chaperone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Animal and plant cells contain a family of constitutively expressed HSP-70 cognate proteins that are localized in different subcellular locations and are presumed to play a role in protein folding and transport. Utilizing antibodies raised against the yeast endoplasmicreticulum-localized HSP-70 cognate termed BiP/GRP-78, as well as antibodies raised against the Escherichia coli HSP-70 protein DnaK, we have identified and characterized a large family of closely related proteins in wheat. One protein band of 78 kDa that is apparently closely related to yeast BiP was localized in the endoplasmic reticulum. This band cross-reacted with the yeast BiP but not with the DnaK-specific antibodies. The yeast BiP antibodies also recognized a cytoplasmic protein of 70 kDa that is probably related to the HSC-70 cognate proteins. These two proteins were further confirmed as HSP-70 cognates by their ability to bind to an ATP-agarose column. Probing of proteins from purified wheat mitochondrial preparations with the yeast BiP and DnaK-specific antibodies showed that this organelle contained a family of HSP-70-related proteins. The yeast BiP antibodies recognized two mitochondrial proteins of 60 and 58 kDa, but failed to detect any protein in the size rang of 70 to 80 kDa. However, the presence of immunologically distinct proteins of 90 and 78 kDa, as well as of lower molecular weight from this family in the mitochondria, was shown by probing with the DnaK-specific antibodies. A new protein of 30 kDa, cross-reacting with anti-yeast BiP antibodies, was detected only in developing seeds, close to their maturity. The evolution of HSP-70 cognate proteins in wheat as shown in this study is discussed.
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    Theoretical and applied genetics 82 (1991), S. 674-680 
    ISSN: 1432-2242
    Keywords: Puccinia recondita tritici ; Triticum aestivum ; Rust resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A study of spring bread wheat (Triticum aestivum) germ plasm developed at the International Maize and Wheat Improvement Center (CIMMYT) showed highly significant phenotypic variability for each component of partial resistance (namely, uredial appearance period, latency period, uredial number and uredial size) to Puccinia recondita f. sp. tritici. All of the wheat genotypes displayed longer uredial appearance and latency periods and decreased uredial number and uredial size when compared to the susceptible check cultivar ‘Morocco’. Positive correlations between uredial appearance period and latency period, and uredial number and uredial size, and negative correlations between uredial appearance and latency periods and uredial number and uredial size, inclusive, suggested that the components of partial resistance were either tightly linked or under pleiotropic genetic control. Compared to ‘Morocco’, all entries had slow disease progress in the field and variation occurred in the germ plasm for the area under the leaf rust progress curve. Disease progress was negatively correlated with uredial appearance and latency periods, whereas a positive correlation was observed with uredial number and uredial size. Certain genotypes displayed high levels of partial resistance resulting in low disease incidence in the field.
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  • 168
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    Theoretical and applied genetics 81 (1991), S. 312-316 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Agropyron michnoi ; Intergeneric hybrid ; Chromosome pairing ; Self-fertile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intergeneric hybrids between Triticum aestivum cv ‘Chinese Spring’ (2n=6x=42, AABBDD) and Agropyron michnoi Roshev. (2n=4x=28, PPPP) were obtained by embryo culture. Their spike characteristics were similar to those of common wheat but, unlike their parents, they were long-awned. The average meiotic chromosome pairing at MI of F1 hybrids was: 6.39 I +3.75 rodII+8.64 ringII+0.81 III+0.30 IV+0.04 V, the bivalent and multivalent formation of which was much higher than expected from the genomic formulae. It is especially worthwhile to note that the F1 hybrids were self-fertile, self set being 0.15%, and seeds were easily obtained from the backcross of f1 plants with hexaploid and tetraploid wheats; here the seed set was more than 20.0%. The polyploid taxa and the position of A. Michnoi in Agropyron are discussed.
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  • 169
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Triticum monococcum ; blue aleurone color ; Chromosome substitution/translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Meiotic chromosome pairing and Giemsa C-banding analyses in crosses of several European blue-grained wheat strains with Chinese Spring double ditelosomic and other aneuploid lines showed that Triticum aestivum Blaukorn strains “Berlin,” “Probstdorf,” “Tschermak,” and “Weihenstephan” are chromosome substitutions, in which the complete wheat chromosome 4A pair is replaced, whereas the strains “Brünn” and “Moskau” are 4B substitutions. The alien chromosome pair in all of these strains is an A genome chromosome (4A) from diploid Triticum monococcum or T. boeoticum not present in common tetraploid and hexaploid cultivated wheats. The Blaukorn strain Weihenstephan “W 70a86” possesses, in addition to a rye chromosome pair 5R compensating for the loss of part of chromosome 5D, a 4A/5DL translocation replacing chromosome pair 4B of wheat.
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  • 170
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    European journal of plant pathology 97 (1991), S. 275-288 
    ISSN: 1573-8469
    Keywords: Triticum aestivum ; brown rust ; monitoring ; epidemiology ; yield loss ; disease management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Epidemics ofPuccinia recondita and resulting yield loss of winter wheat were studied in field experiments over three seasons in the Netherlands. Results are reported and used to construct an advisory model for control of brown rust, based on rust monitoring. If the fraction of leaves with rust (I) at a certain development stage is determined, the average number of rust sori per leaf (M) was estimated by: M=EXP(1.84+1.39ln(ln[1/(1-I)]). The final number of sori per leaf (Mf) at early dough was forecast by an exponential growth: Mf=M·EXP(RGR·t). The relative growth rate (RGR) averaged 0.163/day and the forecasting period, t, until early dough, was derived from published data. The forecast number of sori-days per leaf (S, AUDPC-value) was then obtained by: S=(Mf-M)/RGR. Yield loss (kg/ha) by brown rust was 1.15 times the number of sori-days per leaf at low rust intensities. The efficacy of the fungicides used was 85%. The forecast avoidable yield loss (L, kg/ha) was calculated by: L=0.85 (1.15 S). Economic thresholds for brown rust control at different development stages are given for Dutch wheat fields at a cost level of 270 kg/ha for one fungicide application.
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  • 171
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    Euphytica 59 (1991), S. 1-8 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; root ; combining ability ; correlation ; dry matter ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat root characters which influence vital plant processes have scarcely been explored for their genetic control. This study was conducted to i) examine the diversity of root traits and associated shoot traits in spring wheat (Triticum aestivum L.) cultivars; ii) study the nature of genetic control of selected traits; and iii) examine associations among root and shoot traits. Three experiments were conducted in the greenhouse with plants grown in the vermiculite medium in clear plastic tubes. In the first experiment, 42 spring wheat cultivars were grown for three weeks and measurements were taken on root length, leaf length, root number, leaf number, root dry weight, and top dry weight. In the second study, 15F1's originating from a partial diallel mating of six cultivars along with the parents were evaluated for 4 weeks. The data on root length, leaf length, and root number were subjected to diallel analysis according to Griffing's method 4, fixed model. A third experiment consisted of studying 2 F2 populations with 141 plants per population. In the first study the 42 cultivars exhibited a wide range of variation for all six traits. Diallel analysis revealed significant effects of both general combining ability (GCA) and specific combining ability (SCA) for root length, leaf length, and root number. Parents with high GCA estimates were identified. Significant positive correlation coefficients were found among root and shoot traits. Analysis of F2's for root length indicated quantitative nature of inheritance of root length.
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  • 172
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum compactum ; club wheat ; Triticum spelta ; spelt wheat ; classification ; genetic diversity ; landraces ; cultivars ; USA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In 1922 Clark, Martin and Ball published descriptions of 207 hexaploid wheat landraces and improved cultivars, collected in the USA: 189 bread wheat accessions (Triticum aestivum), 24 club wheats (T. compactum) and four spelt wheats (T. spelta). After omitting 24 bread wheat accessions with identical descriptions as other accessions, the descriptions were used here to classify the remaining 183 accessions into five clusters on the basis of 10 selected characters. Clusters 1 and 3 include mainly accessions introduced from North and West Europe. These accessions must derive from the European Zeeuwse and Gelderse landrace groups. Hence most of the accessions belonging to these two clusters belong to the North and West European heritage. Cluster 2 includes most of the accesions introduced from Australia and Canada, or belonging to the club wheats. Most accessions from the USSR1 are included in cluster 4. Here we also find Turkey and related landraces. All but one pubescent accessions are included in cluster 5. The wide variation of US wheats, described in 1922, forms the basis of the Foundation Germplasm wheats as defined by Cox (1991). Apparently no hard white winter wheat landraces and cultivars were described.
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  • 173
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; hybridization ; pollen-tube-growth ; wide-crosses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five- to seven-day-old ovaries from Triticum aestivum vars Hope (non-crossable), Chinese Spring (CS, crossable) and the chromosomal substitution line Chinese Spring/Hope 5B (CS/H5B) were used to test the hypothesis that an ovarian substance inhibits rye (Secale cereale var Wrens) pollen tube growth. In vitro assays on a semi-solid medium demonstrated that a soluble, dialyzed lysate from Hope or CS/H5B ovaries inhibited rye pollen tube elongation significantly more than a similar lysate from CS ovaries (26%, 13% and 5% respectively compared to controls). When only the 20 longest tubes from each sample were included in the analysis the relationship was maintained (33%, 22% and 15% for Hope, CS/H5B and CS respectively). Heating the Hope lysate at 100° C for 10 min reduced the inhibition from 26% to 14% for all pollen tubes measured (n = 51 per sample) and from 33% to 20% when only the 20 longest pollen tubes per sample were analyzed. Isoelectric focusing followed by sodium dodecyl sulfate polyacrylamide electrophoresis (IEF/SDS-PAGE) and non-equilibrium pH gradient electrophoresis (NEPHGE) revealed several differences in the polypeptide profiles of soluble lysates from vars Hope, CS and CS/H5B. While two striking differences were observed, a 50 kilodalton (kD) polypeptide with an isoelectric point (pI) of approximately 8.5 and 100 kD polypeptide (pI∼4) from CS lysates which were considerably reduced in lysates of Hope and CS/H5B, none were obviously associated with the Hope 5B chromosome and therefore cross-incompatibility.
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  • 174
    ISSN: 1573-5060
    Keywords: bread wheat ; Erysiphe graminis ; powdery mildew ; Triticum aestivum ; Triticum dicoccoides ; wild emmer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A new source of resistance to wheat powdery mildew caused by Erysiphe graminis has been transferred to hexaploid bread wheat, Triticum aestivum, from the wild tetraploid wheat, Triticum dicoccoides. The donor was crossed to bread wheat and the pentaploid progeny was then self-pollinated. Plants having a near stable hexaploid chromosome complement were selected in the F3 progeny and topcrossing and backcrossing of these to a second wheat cultivar to improve the phenotype was undertaken. Monosomic analysis of early backcross lines showed the transferred gene to be located on chromosome 4A. The gene has been designated Pm16.
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  • 175
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    Euphytica 53 (1991), S. 137-141 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; crossability ; Himalayas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crossability of 62 bread wheat accessions (14 landraces from Himachal Pradesh and 48 others) was examined with rye. The 3 rye cultivars did not differ in their relative crossability with 4 of the wheat accessions studied. On the other hand, the wheat cultivars differed greatly among themselves in their crossability with rye. Most of the wheat cultivars showed poor (〈10%) crossability. Two of the 14 landraces from Himachal Pradesh were found to be free from the crossability inhibitors as they showed very high (〉50%) crossability, whereas none of the other 48 cultivars studied was so.
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  • 176
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Aegilops sharonensis ; male sterility ; preferential transmission ; hybrid wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat plants nullisomic for chromosome 4B are male sterile due to the absence of the male fertility gene Ms1. However, plants in which chromosome 4B has been substituted by the preferentially transmitted chromosome 4S1 of Ae. sharonensis are male fertile due to the compensating effect of Ms4 on the alien chromosome. This substitution line has been mutated and three recessive mutation of Ms4 have been selected. Plants homozygous for these mutations are male sterile. The implication of these mutations for hybrid wheat production is discussed.
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  • 177
    ISSN: 1573-5060
    Keywords: boron ; inheritance ; tolerance ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The genetic control of tolerance of wheat to high concentrations of soil boron was studied for five genotypes. Each genotype represented one of five categories of response to high levels of boron, ranging from very sensitive to tolerant. Tolerance to boron was expressed as a partially dominant character, although the response of an F1 hybrid, relative to the parents, varied with the level of boron applied. The F1 hybrids responded similarly to the more tolerant parent at low B treatments and intermediate to the parents at higher treatments. Ratios consistent with monogenic segregation were observed for the F2 and F3 generations for the combinations (WI*MMC) × Kenya Farmer, Warigal × (WI*MMC) and Halberd × Warigal. The three genes, Bo1, Bo2 and Bo3, while transgressive segregation between two tolerant genotypes, G61450 and Halberd, suggested a fourth locus controlling tolerance to boron.
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  • 178
    ISSN: 1573-5060
    Keywords: Canada ; genotypic differences ; manganese toxicity ; manganese tolerance ; origin ; pedigree ; screening ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Breeding wheat (Triticum aestivum L.) for tolerance to manganese (Mn) might be in some cases more feasible and economical than use of soil amendments. As part of research on the heritability of Mn tolerance, a study on the level of Mn tolerance in Canadian wheat cultivars and its probable origin was accomplished by analysis of cultivar pedigrees and drawing phylogenetic maps to discern filial relationships. Cultivar tolerance to Mn was determined by relative root weight (RRW) in solution culture in the presence of 500 μM Mn. A total of 91 cultivars were screened, 76 of which were Canadian. These data, together with data from another 28 cultivars reported in the literature, were used to draw two pedigree maps, a map for Canadian cultivars only, and a map for the Mn-tolerant Canadian cultivars Norquay and Laura. Results indicated a range of tolerance to Mn among Canadian cultivars. Manganese tolerance, found in either Canadian or foreign germplasm, and of either recent or older selection or origin, seems to have originated from land races from Rio Grande do Sul, the southernmost state of Brazil. Tolerance may have been introduced into Canadian germplasm directly by the use of Brazilian cultivars as parents, or indirectly by the introduction of Mexican germplasm with Brazilian parentages. This information will help the plant breeder to develop plant breeding systems, and may also help in the study of the mechanisms for Mn tolerance in wheat.
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  • 179
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Diuraphis noxia ; Russian wheat aphid ; host plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Russian wheat aphid (Diuraphis noxia, Mordvilko) (RWA) is responsible for significant economic damage to cereal crops in arid and semi-arid environments. In this research 20 red winter wheats originating from Iran were evaluated for resistance to RWA. Leaf rolling, leaf folding, and leaf chlorosis were measured using 0 to 3 scales. An overall mean damage score was calculated as the average of the three measured damage symptoms. Plants from seven central Asian accessions (PI222666, PI222668, PI225226, PI225267, PI225271, PI243630, and PI243642) had mean damage scores significantly lower (p 〈 0.001) than ‘Stephens’ wheat (RWA susceptible) and not significantly different from ‘Border’ oat (RWA resistant). These results are consistent with previous studies which found a high frequency of resistant wheats collected from the central Asian region.
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  • 180
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    Euphytica 58 (1991), S. 57-63 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; cold hardiness ; cultivar x tillage interaction ; tillage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Conservation tillage in the Great Plains of North America has increased in recent years, in part, to control soil erosion, increase soil water storage and reduce production costs. No-till production is recommended for winter wheat (Triticum aestivum L.) in northern latitudes for the purpose of trapping snow and protecting the crop from freezing temperatures. A 5 year field study was conducted at four North Dakota locations from 1984–85 through 1988–89 to determine if significant cultivar x tillage interactions existed for grain yield of winter wheat. Fourteen hard red winter wheat cultivars were planted in both conventional-till and no-till systems. Significant cultivar x tillage interactions were obtained for grain yield from both a weighted analysis across all environments and from individual analyses of 12 of the 16 environments in which wheat survived under both tillage systems (P〈0.05). Regression of cultivar yield on mean environment yield produced b values that varied little between conventional till and no-till for 10 of the 14 cultivars. The top five yielding cultivars were the same for both tillage systems and had b values 〉 1.0. Although the cultivar x tillage interaction may be a result of differential winter survival rather than tillage per se, this study suggests that selection for winter wheats with superior yield performance can be conducted under either no-till or conventional-till systems.
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  • 181
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    Euphytica 58 (1991), S. 171-181 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia striiformis ; yellow rust ; stripe rust ; host plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four spring wheat (Triticum aestivum L.) varieties differing in origin and reaction in the seedling stage to pathotype CDL-6 (extant in California) were intercrossed and examined in greenhouse conditions in F1, F2, and F3 generations. Digenic and transgressive segregation was found in all crosses. The four varieties each had infection types (1 immune, 9 susceptible) and putative resistance genes as follows: ‘Anza’, IT 7, YrA; ‘Glennson 81’, IT 2, Yr9; ‘Yecora Rojo’, IT 6, YrC; and ‘Ollanta’, IT 4–6, YrL. ‘Anza’ was classified as susceptible, ‘Yecora Rojo’ and ‘Ollanta’ as intermediate in seedling resistance, and ‘Glennson 81’ as resistant in the seedling stage.
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  • 182
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Secale cereale ; rye ; day-length insensitivity ; semi-dwarfness ; tissue culture response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of genes affecting the ‘plant growth habit’ in wheat (Rht8 and Ppd1) and rye (ct1 and ct2) on tissue culture response was studied using immature embryos. Whereas the semi-dwarfing gene Rht8 seems to promote only a minor effect, the day-length sensitive allele ppd1 determined a major increase in callus growth and regeneration ability. With regards to their tissue culture efficiency, the four alleles studied could be ranked as follows: ppd1〉Rht8〉rht8〉Ppd1. In contrast to wheat, the GA insensitive semi-dwarfing genes of rye (ct1 and ct2) appear not to influence in vitro response.
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  • 183
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    Plant and soil 137 (1991), S. 191-199 
    ISSN: 1573-5036
    Keywords: durixerollic calciorthid soil ; evapotranspiration ; plant water relations ; silicon transport soil ; temperature ; Triticum aestivum ; uptake ; water balance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Silicon (Si) content in cereal plants and soil-Si solubility may be used to estimate transpiration, assuming passive Si uptake. The hypothesis for passive-Si uptake by the transpiration stream was tested in wheat (Triticum aestivum cv. Stephens) grown on the irrigated Portneuf silt loam soil (Durixerollic calciorthid) near Twin Falls, Idaho. Treatments consisted of 5 levels of plant-available soil water ranging from 244 to 776 mm provided primarily by a line-source sprinkler irrigation system. Evapotranspiration was determined by the water-balance method and water uptake was calculated from evapotranspiration, shading, and duration of wet-surface soil. Water extraction occurred from the 0 to 150-cm zone in which equilibrium Si solubility (20°C) was 15 mg Si L−1 in the Ap and Bk (0–58 cm depth) and 23 mg Si L−1 in the Bkq (58–165 cm depth). At plant maturity, total Si uptake ranged from 10 to 32 g m−2, above-ground dry matter from 1200 to 2100 g m−2 and transpiration from 227 to 546 kg m−2. Silicon uptake was correlated with transpiration (Siup=−07+06T, r2=0.85) and dry matter yield with evapotranspiration (Y=119+303ET, r2=0.96). Actual Si uptake was 2.4 to 4.7 times that accounted for by passive uptake, supporting designation of wheat as a Si accumulator. The ratio of Si uptake to water uptake increased with soil moisture. The confirmation of active Si uptake precludes using Si uptake to estimate water use by wheat.
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  • 184
    ISSN: 1573-5036
    Keywords: aluminium tolerance ; cation-anion balance ; phosphate translocation ; split-root ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seedlings of two cultivars of wheat (Triticum aestivum L.) differing in tolerance to aluminium (Al) were grown using a split-root sand/soil culture technique. Each culture tube was divided horizontally into a surface (0–150 mm) compartment and a subsurface (150–250 mm) compartment separated by a root-permeable paraffin wax barrier. Thus phosphorus (P) supplied to surface roots could not percolate or diffuse into the soil in the subsurface compartment. The soil in the subsurface compartment was divided into ‘rhizosphere’ and ‘non-rhizosphere’ zones using a porous (5 μm) membrane. Root growth of both cultivars into the subsurface zone was enhanced by increased P supply to surface roots, but did not conform to known relationships between root growth and soil pH, extractable-Al, or pH, Al or P concentrations in soil solution. Concentrations of Al in soil solution in the rhizosphere were greater than those in solution in the bulk soil. Concentrations of Al reactive with pyrocatechol violet (30s-RRAI) in the rhizosphere soil solution were generally greater than those in non-rhizosphere soil. With the Al-sensitive cultivar, root dry weight and length increased as concentrations of RRAl in the rhizosphere soil solution increased. Increased concentrations of Al in rhizosphere soil solutions were not related to the presence of organic ligands in solution. The effect of P in promoting root penetration into the acidic subsurface stratum was not related to differential attainment of maturity by the plant shoots, but appeared to be related to the effect of P in enhancing the rate of root growth. Thus, suboptimal supply of P to the surface roots of a plant, even at levels sufficient to preclude development of nutritional (P) stress symptoms, may seriously reduce tolerance to Al, and hence diminish the ability of roots to penetrate into acidic subsoils.
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  • 185
    ISSN: 1573-5036
    Keywords: aglycone ; allelopathy ; arabinose ; bioassay ; Bromus secalinus ; dormancy rating ; Medicago sativa ; saponins ; thin-layer chromatography (TLC) ; Trichoderma viride ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat, cheat and Trichoderma viride bioassays were used to establish the relationship between the content of biologically active saponins in the roots and the degree of winter dormancy and/or time of six cultivars of alfalfa over the period January to August, 1988. Wheat and cheat bioassay results indicated no significant difforences among cultivars, whereas T. viride was inhibited most by extracts of roots collected during months with high rainfall and rapid growth. Cheast seedling roots were inhibited 8–10% more than those of wheat seedling roots indicating that alfalfa root saponins were more effective as allelopathic compounds in preventing growth of cheat than that of wheat alone. An average of 14 different saponins per cultivar were separated by thin-layer chromatograms. Saponins, and the aglycones produced by acid hydrolysis of the May samples, were separated by thin-layer chromatography. The conclusion is that the amount, structure, and type of saponins present in alfalfa roots vary with time.
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  • 186
    ISSN: 1573-5036
    Keywords: growth promotion ; sterile red fungus ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Enhancement of shoot and root growth of wheat seedlings by a sterile red fungus was evident even when sufficient nutrients were supplied and density of plants per pot was increased (from 15 to 30 plants per pot of 400 g soil). It is likely that production of hormonal-type substances by these fungi may be involved in the phenomenon observed.
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    Plant and soil 136 (1991), S. 183-193 
    ISSN: 1573-5036
    Keywords: diagnosis ; distribution ; magnesium deficiency ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The aims of this study were to describe the distribution of magnesium (Mg) and its retranslocation within wheat, in order to develop diagnostic procedures for Mg deficiency. Plants were grown in solution culture with both constant supply (0, 5, 10, 20, 40, 80, 160 μMMg) and discontinued supply (40 μM and 160 μM decreased to nil). Magnesium was depleted from old leaves when Mg supply to the roots was halted. However, initial deficiency symptoms occurred on young leaves under constant but inadequate supply, contrasting with previous reports. Magnesium concentrations were also lower in young leaves compared to old leaves. Symptoms of yellowing and necrosis occurred if the leaf tissue contained 〈1194 μgg−1, irrespective of leaf age. The minimum Mg concentration in whole shoots associated with maximum shoot weight was 932 μgg−1; for the youngest emerged blade (YEB) it was 861 μgg−1. Symptoms were apparent on the young leaf before a reduction in shoot weight was measurable. The concentration of Mg in the YEB and whole shoot were better related to solution Mg concentration than was the Mg concentration in the old leaf.
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  • 188
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; drought resistance ; yield ; selection ; grain filling ; kernel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Post-anthesis chemical desiccation of wheat (Triticum aestivum L.) plants in the field eliminates transtent photosynthesis by killing all green tissues, thus revealing the plant's capacity for grain filling from stored stem reserves, as the case is for post-anthesis stress such as drought or leaf diseases. This study was conducted to investigate whether mass selection for large kernels under chemical desiccation would lead to the improve ment of grain filling in the absence of transient photosynthesis. Six crosses of common spring wheat were subjected to three cycles of mass selection from F2 through F1 when selection was performed for large kernels by sieving grains from plants that were erther chemically desiccated after anthesis, or not (controls). The resulting 36 bulks (six crosses by three selection cycles by two selection environments) were compared with their respective F2 base populations, when tested with and without chemical desiccation. Selection for large kernels under potential conditions (without chemical desiccation) did not improve kernel weight under potnetial conditions, evidently because these materials were lacking in genetic variation for kernel weight under potential conditions. In four of the crosses, 3rd cycle selection for large kernels under potential conditions decreased kernel weight under chemical desiccation. On the other hand, selection for large kernels under chemical desiccation was effective in improving kernel weight and test weight under chemical desiccation, depending on the cross and the selection cycle, with no genetic shift in mean days to heading or mean plant height. Selection for large kernels under chemical desiccation was also effective in some cases in increasing kernel weight under potential conditions. The results are interpreted to show that selection under potential conditions and under chemical desiccation operate on two different sources for grain filling, namely transient photosynthesis and stem reserve utilization, respectively. In order to expose genetic variability for stem reserve utilization to selection pressure, transient photosynthesis must be eliminated, as done by chemical desiccation in this study.
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  • 189
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    Euphytica 54 (1991), S. 285-295 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; gliadins ; APAGE ; multiple allelism ; variation ; Yugoslavia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The complete gliadin allele composition of 57 Yugoslav common winter wheat cultivars was studied. Large differences were found in gliadin genotypes among cultivars bred at different Yugoslav breeding centres as well as between early and recent wheats bred in the Novi Sad breeding centre. Yugoslav wheats have limited variation in gliadin alleles, of which Gli-A1a, Gli-B1b, Gli-B1l, Gli-D1b, Gli-A2e, Gli-B2b, Gli-D2a are shown to be the most frequent. Examples of favoured alleles to new cultivars are described. 40% of the studied wheat cultivars have natural biotypes differing in gliadin allele composition. The frequency of cultivars with the biotypes differs greatly among breeding centres.
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  • 190
    ISSN: 1573-5060
    Keywords: adult plant resistance ; seedling resistance ; Septoria nodorum ; septoria nodorum blotch ; X Triticosecale ; triticale ; Triticum aestivum ; wheat ; T. spelta ; spelt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary On average, the cereal species studied were susceptible to septoria nodorum blotch (SNB), except for spring triticale on leaf and head and winter titicale on leaf, that appeared to be significantly more resistant, than the other ones. In all three species the SNB response of the adult plants was to a limited extent only predicted by the reaction on first leaf seedlings. In most cases it was impossible to predict the response to SNB of adult plants on the basis of seedling reaction. Correlations between the adult plant stage and the seedling stage, or detached seedling leaves, appears not to be sufficient for use in practical breeding work. A reversal of reaction to SNB was even found between the above growth stages in studied spring and winter wheat varieties.
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  • 191
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    Euphytica 55 (1991), S. 229-234 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; harvest index ; diallel analysis ; genotypic and phenotypic correlations ; assimilate partitioning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Cereal breeders have used harvest index (HI) as a selection criterion in segregating generations to identify physiologically superior lines with improved partitioning of total assimilate into grain. Information on combining ability for HI of the hard red winter wheat (Triticum aestivum L.) cultivars of the Southern Great Plains is not available. A study was undertaken to examine HI of seven genetically diverse winter wheat parents, evaluate their general combining ability (GCA) and specific combining ability (SCA) effects, and study correlations of HI with several agronomic traits. The seven parents were crossed in a half-diallel mating design to produce 21 crosses. The F1's, their F2 progenies, and the parents were evaluated in replicated field tests at Stillwater and at Lahoma, OK. The combining ability analysis was performed using Griffing's Method 4, Model 1. The results showed significant variation among parents for HI. The GCA and the SCA effects were mostly inconsistent between generations and between environments. However, parents with consistently high HI and positive GCA estimates were identified. The progeny with high HI mostly resulted from parents with high GCA estimates. The correlations between HI and agronomic traits indicated that improvement in HI should also result in high grain yield, early maturity, and short plant height.
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  • 192
    ISSN: 1573-5060
    Keywords: HMW glutenin subunits ; bread-making quality ; Triticum aestivum ; wheat ; epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The relation has been studied between the high molecular weight glutenin (HMWg) subunit alleles and the bread-making quality of 226 lines of winter wheat (T. aestivum L.), grown in The Netherlands. The lines represented a wide range of genetic backgrounds, and had not been selected for quality, in contrast to the established varieties used by other authors. The variation in HMWg subunit genotypes accounted for about 20% of the total variation in loaf volume among the lines. Most important was the allelic variation at the Glu-D1 locus. The Glu-D1 allele encoding the subunits 5+10 was superior to its allelic counterpart, encoding 2+12. The difference in average of loaf volume between groups of lines containing 5+10 or 2+12 was negatively related with protein content of the flours. When protein content was below 9.2%, no effect of allelic variation at the Glu-D1 locus was present. Epistatic effects between the Glu-I loci also contributed to the variation in loaf volume of the lines: i.e. the effect of allelic variation at Glu-A1 and Glu-B1 depended on the allele present at the Glu-D1. The contribution of the epistatic effects was about half the contribution of the additive effects, and should therefore be included in predictive models for bread-making quality.
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  • 193
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    Euphytica 57 (1991), S. 9-17 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; winter wheat ; intrinsic earliness ; photoperiod ; vernalization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Development in wheat is strongly controlled by sensitivity to vernalization and photoperiod, and to a lesser degree by non-vernalizing temperature and intrinsic earliness. A method to measure effect of vernalization in wheats with winter habit is described. Twenty seven wheats with winter habit and eight with spring/facultative habit were studied, comprising breeding lines and cultivars with maturities suited to south-central New South Wales. Effect of vernalization on the development of these wheats was quantified by integrating responses to vernalizing treatments of differing duration. Intrinsic earliness was measured as time for vernalized seedlings to grow to ear emergece in an 18h photoperiod with day/night temperature of 21/16°C, and response to photoperiod as the difference in time to ear emergence between 9 and 18h daylengths. Integrated response to vernalization is sensitive to both cumulative and thresh-hold responses and is applicable to wheats of all habit type. Integrated response to vernalization and intrinsic earliness were positively associated within wheats with winter habit. Wheats were largely of restricted origin, so that there were few allelic differences at Vrn loci to disrupt this association, which suggests intrinsic earliness may modify response to vernalization. Though integrated response to vernalization was measured with artificial treatments, it was strongly associated with ear emergence for wheats with winter habit when grown at a site in New South Wales.
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  • 194
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita tritici ; leaf rust ; rust resistance ; slow rusting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genes conferring low seedling reaction to Mexican pathotypes of Puccinia recondita f. sp. tritici in 71 bread wheat (Triticum aestivum L.) cultivars from India and Pakistan were postulated. In total, 9 known and one unknown genes were identified, either singly or in combination: Lr1 (in 20 cultivars), Lr3 (5), Lr10 (21), Lr11 (1), Lr13 (43), Lr17 (5), Lr23 (14), Lr26 (2), Lr27 + Lr31 (2), and the unknown gene in 2 cultivars. Additional temperature-sensitive seedling resistance appeared to occur in 27 cultivars. This resistance in at least 15 cultivars appeared to be due to Lr34. Area under the disease progress curve (AUDPC) for these 27 cultivars indicated variable levels of adult plant resistance. Several other cultivars with high seedling infection types to one or more of the predominant field pathotypes were also partially resistant in the field. High levels of adult plant resistance occurred in some cultivars even in the absence of known seedling resistance genes with major effects.
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  • 195
    ISSN: 1573-5060
    Keywords: Adult plant resistance ; infection frequency ; latency period ; partial resistance ; Puccinia recondita f.sp. tritici ; race-specificity ; temperature sensitivity ; Triticum aestivum ; wheat ; wheat leaf rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Components of partial resistance, infection frequency and latency period, were determined in 71 winter and spring wheat genotypes in the seedling stage, after infection with three races of leaf rust (‘Felix 3B’, ‘Clement B’ and ‘Betuwe 85C’) at three different day/night temperature regimes (24/18°C, 18/12°C and 12/6°C). The genotypes were split into two groups and two separate experiments were carried out. Five genotypes, SVP 84039, Akabozu, Banco, BH 1146 and Orso, conferred a low infection frequency and a long latency period and Westphal 12A a long latency period, indicating a relatively high level of partial resistance. The correlation coefficient between infection frequency and latency period was low. Race-specificity was not found. There was a significant temperature effect on the latency period. In the second experiment the temperature x genotype interaction was significant. Temperature-response functions of transformed data demonstrated that the latency periods of four relatively resistant genotypes, Westphal 12A, Banco, BH 1146 and Orso and of Sarno and Mirela were most sensitive to temperature. The range between the genotypes with the longest and the shortest latency period was highest at 12°C. Therefore, low temperature regimes are preferred to distinguish differences in level of partial resistance.
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  • 196
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    Euphytica 58 (1991), S. 165-170 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; choice of parents ; distance analysis ; standardized potence ; character selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three groups of characters selected using three methods were used to calculate genetic distance among two groups of winter wheat lines using Euclidean distance based on principal components. These wheat lines were then clustered by the unweighted pair-group arithmetic average method. The relationship between genetic distance and standardized potence was seemingly influenced by genetic materials. In order to obtain promising crosses, it is advisable to select parents from intercluster rather than intracluster materials. The results suggested that the repeatability method was better than the conventional and weighting methods for selecting characters for distance analysis.
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  • 197
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    Plant cell, tissue and organ culture 26 (1991), S. 141-146 
    ISSN: 1573-5044
    Keywords: calluses mutagenesis ; embryo mutagenesis ; EMS somatic effect ; immature ; embryo mutagenesis ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mutation experiments require careful selection of a mutagen with characteristics suited to the tissue source and mutagenesis objective, and an appropriate treatment regime. The objectives of the present investigation were:-to compare the ability of immature embryos to initiate calluses and calluses derived from immature embryos to survive and grow after being treated with ethyl methanesulfonate (EMS) doses of 0.2, 0.4, 0.8, 1.2, and 1.6% (v/v) and-to compare these results with those published for seed mutagenesis. To determine if the response of tissue source to EMS treatment varied with genotype, tissues from two spring wheat cultivars, ‘Angus’ and ‘Pavon 76’, were used. The combined analysis of variance detected highly significant differences (p≤0.01) among doses. The higher the dose, the lower the tissue survival in each tissue source. No significant differences were detected between cultivars, tissue sources, in the cultivar by dose interaction, tissue source by dose interaction, tissue source by cultivar interaction, or tissue source by cultivar by dose interaction. Hence, both cultivars and tissue sources responded similarly to EMS doses. The predicted LD20 are 0.35%±0.08% for the immature embryo treatment, and 0.36%±0.10% for the callus treatment. The predicted LD50 are 0.82%±0.13% for the immature embryo treatment, and 0.77%±0.13% for the callus treatment. These results were very similar to published results for seed mutagenesis, hence seed mutagenesis research may be applicable to immature embryo and callus mutagenesis.
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  • 198
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    Plant cell, tissue and organ culture 24 (1991), S. 233-236 
    ISSN: 1573-5044
    Keywords: anther culture ; cultivars ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-two cultivars and lines of winter and spring wheat (Triticum aestivum L.) were studied, most for the first time, for their anther culture response. The response was genotype dependent. Plants grown in the field gave higher callus induction frequency than those grown in the greenhouse and the controlled environment chamber. Donor plants grown in a season of low drought stress as compared to a season of severe drought stress resulted in a higher frequency of callus induction. Spherical microcalli were observed in two wheat genotypes in some of only those anthers that were placed with only one loculus in contact with the medium. Wheat lines that were more responsive to anther culture were identified.
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  • 199
    ISSN: 1573-5060
    Keywords: Introgression ; leaf rust ; Puccinia spp. ; rust resistance ; species-specific probes ; stem rust ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Originally developed for resistance to greenbug derived from Insave rye, Amigo wheat carries two genes for resistance to stem rust. One of these genes is associated with a rye chromosome 1RS segment carrying the Sec-1 protein marker and presumably greenbug resistance. The second gene which is genetically linked to leaf rust resistance is associated with an Agropyron-derived segment. Rust tests in Canada confirmed that these genes were Sr24 and Lr24. In contrast to Agent and certain 3D/Ag derivatives from Dr. E.R. Sears, the Amigo source of Sr24/Lr24 freely recombined with white seed colour during backcrossing.
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  • 200
    ISSN: 1573-5060
    Keywords: Fusarium resistance ; Fusarium spp. ; wheat ; Triticum aestivum ; double-layer technique ; in vitro selection ; somaclonal variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Calluses of spring and winter wheats (Triticum aestivum L.) were selected for Fusarium resistance in vitro, using the double-layer culture technique. Potato-dextrose agar medium in vials was inoculated with mycelia of Fusarium graminearum and F. culmorum. After one week, fungal cells were killed by autoclaving and the agar medium containing the thermostable toxic metabolites was overlayered with MS callus-growing medium. Later, wheat calluses were placed on the upper medium for 4–5 weeks, and from the surviving calluses plants were regenerated. R2 seedling populations from self-fertilized R1 plants of 4 varieties were tested for Fusarium resistance by artificial infections in the greenhouse, and 3% of the regenerated R2 plants have been found to be more resistant than the original cultivars.
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