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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 60 (1991), S. 173-182 
    ISSN: 1570-7458
    Keywords: Genetics ; evolution ; host adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When populations are exposed to different environments, evolutionary processes can lead either to genetically differentiated strains or to the appearance of increased generalism at the individual level. For evolution to occur, genetic variability in performance in different environments is required. Here, intraspecific genetic variation across environments was estimated in the flour beetle Tribolium castaneum (Herbst) by comparing the responses of two strains of T. castaneum to different flour types. Replicated groups from each strain were allowed to develop on either the standard whole wheat medium or on one of four novel flours (wheat, rice, corn and oat). In several of the novel flours, clear differences in mean development time or population size of one or both strains were seen relative to performance in the standard medium. Moreover, the strains differed significantly in their phenotypic responses to the flours. One strain did particularly poorly on oat flour. Reduced oviposition, reduced larval survivorship and increased larval cannibalism were examined as possible causes of the low productivity on oat flour. These three factors accounted for about 70% of the reduction in population size when this strain oviposited and developed in oat flour. The difference between these two outbred strains in response to these five flours suggests that genetic variation in resource use is present within T. castaneum and may also be present within strains and natural populations in grain storage facilities. Such variation would permit an evolutionary response to selection in multiple environments (flours). This process has agricultural implications when several types of grain are stored in a single location because it could eventually lead to the evolution of highly generalized populations of T. castaneum, an important pest of stored products.
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  • 2
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    Entomologia experimentalis et applicata 34 (1983), S. 215-220 
    ISSN: 1570-7458
    Keywords: Oviposition preference ; Genetics ; Oncopeltus fasciatus ; Hemiptera ; Lygaeidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Bei Oncopeltus fasciatus von 6 verschiedenen Herkünften und deren F1 und F2 Hybriden wurde die bevorzugte Eiablagestelle untersucht. In drei Versuchsreihen erwiesen sich dabei alle an einer Lokalität gesammelten Proben als statistisch homogen. Ferner bilderen alle in Nordamerika gesammelten Proben eine statistisch homogene Gruppe, die sich von der puertoricanischen Herkunft unterschied. F1 and F2 Hybriden nicht puertoricanischer Herkunft unterschieden sich nicht wesentlich voneinander. Hybriden zwischen puertoricanischen und nordame-rikanischen Herkünften zeigten in F1 eine dominante Neigung in Richtung der puertoricanischen Eiablagepräferenz; diese Neigung verlor sich in F2. Aufgrund dieses Verlusts wurde geschlossen, dass die Eiablagepräferenz polygenisch bestimmt ist.
    Notes: Abstract Oviposition site preferences were examined in descendents of milkweed bugs collected in six geographic areas and in their F1 and F2 hybrids. Within an area, samples were stastically homogeneous in oviposition preference across three trials. All samples from within the continental USA formed a statistically homogeneous group and, as a group, were different in oviposition preference from the Puerto Rican collection. F1 and F2 hybrids with no Puerto Rican ancestry were not significantly different from each other in oviposition preference. Hybrids with Puerto Rican ancestry exhibited a “dominance” deviation in favor of the Puerto rican oviposition site preference in the F1 generation, but lost that deviation in the F2 generation. This loss was interpreted as evidence that oviposition preference in Oncopeltus fasciatus was polygenically controlled. Probable selection pressures affecting oviposition preferences in O. fasciatus and the advantages of polygenic control of this trait were discussed.
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  • 3
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    Journal of insect behavior 3 (1990), S. 579-587 
    ISSN: 1572-8889
    Keywords: Genetics ; polymorphism ; reproductive isolation ; hovering behavior ; Tabanus nigrovittatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The salt marsh horse fly, Tabanus nigrovittatusMacquart, exhibits two nonoverlapping daily periods of hovering and mating activity, which are correlated with different environmental temperatures. Allelic and genotypic frequencies of hovering males collected during the two periods were compared by electrophoresis of three polymorphic enzyme loci. Approximately 26% of early-hovering males possessed a Pgmallozyme that was absent in our sample of late-hovering males. However, based on other allozyme loci, we found no evidence for reproductive isolation between early and late hoverers. All the genetic data are consistent with the hypothesis that the Pgmpolymorphism is associated with behaviorally and physiologically distinct groups of males that, by all other criteria, form a single Mendelian population.
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  • 4
    ISSN: 1572-8773
    Keywords: Iron transport ; Siderophores ; Pseudomonas putida ; Genetics ; Receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Root-colonizingPseudomonas putida WCS358 enhances growth of potato in part by producing under iron-limiting conditions a yellow-green, fluorescent siderophore designated pseudobactin 358. This siderophore efficiently complexes iron(III) in the rhizosphere, making it less available to certain endemic microorganisms, including phytopathogens, thus inhibiting their growth. At least 15 genes distributed over five gene clusters are required for the biosynthesis of pseudobactin 358. High-affinity iron(III) transport in strain WCS358 is initiated by an 86-kDa outer membrane receptor protein (PupA) which appears to be specific for ferric pseudobactin 358. PupA shares strong similarity with TonB-dependent receptor proteins ofEscherichia coli, which suggests a TonB-like protein in strain WCS358 is required for iron(III) transport. Strain WCS358 possesses a second uptake system for ferric pseudobactin 358 and structurally diverse ferric siderophores produced by other microorganisms. A second receptor gene (pupB) responsible for iron transport from pseudobactin BN7 or pseudobactin BN8 has been identified. The production of this and certain other ferric siderophore receptor proteins requires that strain WCS358 be grown in the presence of these siderophores. An apparent regulatory gene required for the expression ofpupB is located adjacent topupB. Two positive regulatory genes have been identified which can independently activate, under low-iron(III) conditions, transcription of genes coding for the biosynthesis of pseudobactin 358.
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  • 5
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    Cellular and molecular life sciences 50 (1994), S. 429-437 
    ISSN: 1420-9071
    Keywords: Genetics ; ecology ; DNA-transfer ; conjugation ; transformation ; transduction ; transposons ; dormant cells ; epilithon ; microbial colonisation ; symbiosis ; virus resistance ; biosafety ; release of genes ; insults to humanity ; evolution ; biodiversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Genetic ecology is the extension of our modern knowledge in molecular genetics to studies of viability, gene expression and gene movements in natural environments like soils, aquifers and digestive tracts. In such milieux, the horizontal transfer of plasmid-borne genes between phylogenetically distant species has already been found to be much more frequent than had been expected from laboratory experience. For the study of exchanges involving chromosomally-located genes, more has to be learned about the behaviour of transposons in such environments. The results expected from studies in genetic ecology are relevant for considerations of evolution, biodiversity and biosafety. The role of this new field of research in restoring popular confidence in science and in its biotechnological applications is stressed.
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  • 6
    ISSN: 1432-0983
    Keywords: Chlamydomonas ; Chloroplast ; DNA ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The density, molecular weight, and cellular repetition of DNA molecules associated with the β-DNA satellite of the interfertile algae Chlamydomonas eugametos and C. moewusii are reported. The similarities between these values and those for the chloroplast DNA (cpDNA) in the related alga Chlamydomonas reinhardtii indicate that these satellites represent cpDNA. The buoyant densities of C. eugametos and C. moewusii cpDNAs are indistinguishable from one another, as are those of their respective nuclear DNAs. These densities differ slightly from the densities of the homologous components of C. reinhardtii whole cell DNA. All three species differ with respect to additional minor satellite DNAs and low molecular weight DNAs of unknown cellular location. Differences in the Aval and Smal restriction endonuclease fragmentation patterns of C. eugametos and C. moewusii cpDNAs were employed to study the inheritance of cpDNA in an F1 hybrid which had inherited a non-Mendelian streptomycin resistance marker (sr-2) from the C. eugametos mating-type plus (mt +) parent and in two homoplasmic mitotic segregants from a B 1 hybrid (F1 × C. moewusii) which had been initially heteroplasmic for the resistance marker. Although the cpDNA patterns in the F1 hybrid were similar to those of the C. eugametos ml 1 parent, important differences were noted which suggest that recombination between C. eugametos and C. moewusii cpDNA had occurred. Homoplasmic streptomycin resistant and sensitive mitotic segregants recovered from the B1 hybrid product reveal Aval restriction patterns similar to those of the respective resistant and sensitive parents. These data are consistent with the hypothesis that the sr-2 marker is located in cpDNA and that C. eugametos and C. moewusii cpDNA sequences can coexist in the same chloroplast and, at least sometimes, segregate without extensive recombination. The transmission of low molecular weight DNAs characteristic of C. moewusii but of unknown cellular origin shows no direct correlation with the transmission of the sr-2 marker.
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  • 7
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    Journal of molecular evolution 16 (1980), S. 211-267 
    ISSN: 1432-1432
    Keywords: Nucleic acids ; Proteins ; Natural selection ; Genetics ; Nonrandom molecular divergence ; Nonrandom REH theory ; Evolution ; mRNA ; DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary REH theory is extended by deriving the theoretical equations that permit one to analyze the nonrandom molecular divergence of homologous genes and proteins. The nonrandomicities considered are amino acid and base composition, the frequencies with which each of the four nucleotides is replaced by one of the other three, unequal usage of degenerate codons, distribution of fixed base replacements at the three nucleotide positions within codons, and distributions of fixed base replacements among codons. The latter two distributions turn out to dominate the accuracy of genetic distance estimates. The negative binomial density is used to allow for the unequal mutability of different codon sites, and the implications of its two limiting forms, the Poisson and geometric distributions, are considered. It is shown that the fixation intensity — the average number of base replacements per variable codon - is expressible as the simple product of two factors, the first describing the asymmetry of the distribution of base replacements over the gene and the second defining the ratio of the average probability that a codon will fix a mutation to the probability that it will not. Tables are given relating these features to experimentally observable quantities inα hemoglobin,β hemoglobin, myoglobin, cytochromec, and the parvalbumin group of proteins and to the structure of their corre-sponding genes or mRNAs. The principal results are (1) more accurate methods of estimating parameters of evolutionary interest from experimental gene and protein sequence data, and (2) the fact that change in gene and protein structure has been a much less efficient process than previously believed in the sense of requiring many more base replacements to effect a given structural change than earlier estimation procedures had indicated. This inefficiency is directly traceable to Darwinian selection for the nonrandom gene or protein structures necessary for biological function. The application of these methods is illustrated by detailed consideration of the rabbitα -andβ hemoglobin mRNAs and the proteins for which they code. It is found that these two genes are separated by about 425 fixed base replacements, which is a factor of two greater than earlier estimates. The replacements are distributed over approximately 114 codon sites that were free to accept base mutations during the divergence of these two genes.
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  • 8
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    Journal of molecular evolution 15 (1980), S. 79-101 
    ISSN: 1432-1432
    Keywords: Molecules ; Interstellar ; Chemistry ; Isotopes ; Solar system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The study of interstellar molecules broadly includes two areas of interest. One area uses the unique ability of molecules to act as probes of the physical conditions in the cold, dense, visually opaque component of the interstellar medium. The physical properties of this and other components of the interstellar medium are summarized. The other area deals with the chemistry of interstellar molecules, recent aspects of which are emphasized in this review. Gas-phase chemistry, shock chemistry, and grain surface chemistry are discussed in the context of recent observations. No present observations suggest that surface reactions are relevant, but neither can they be ruled out. Ion-molecule reactions are clearly operative, at least for the simpler species. Chemical isotope fractionation is reviewed, andd it is concluded that the complexities of the chemistry allow no cosmological conclusions to be drawn from observations of deuterium in interstellar molecules, while the presence of13C in interstellar molecules permits an estimate of the12C/13C ratio which is consistent with the current concepts of the nucleosynthesis history of the Galaxy. Possible connections between interstellar molecules and the early molecular history of the solar system are discussed.
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  • 9
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    Journal of molecular evolution 17 (1981), S. 167-181 
    ISSN: 1432-1432
    Keywords: Evolution ; Genetics ; REH theory ; Mutations ; Natural selection ; Nucleic acids ; Proteins ; Paleogenetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have independently repeated the computer simulations on which Nei and Tateno (1978) base their criticism of REH theory and have extended the analysis to include mRNAs as well as proteins. The simulation data confirm the correctness of the REH method. The high average value of the fixation intensity μ2 found by Nei and Tateno is due to two factors: 1) they reported only the five replications in which μ2 was high, excluding the forty-five replications containing the more representative data;and 2) the lack of information, inherent to protein sequence data, about fixed mutations at the third nucleotide position within codons, as the values are lower when the estimate is made from the mRNAs that code for the proteins. REH values calculated from protein or nucleic acid data on the basis of the equiprobability of genetic events underestimate, not overestimate, the total fixed mutations. In REH theory the experimental data determine the estimate T2 of the time average number of codons that have been free to fix mutations during a given period of divergence. In the method of Nei and Tateno it is assumed, despite evidence to the contrary, that every amino acid position may fix a mutation. Under the latter assumption, the measure X2 of genetic divergence suggested by Nei and Tateno is not tenable: values of X2 for theα hemoglobin divergences are less than the minimum number of fixed substitutions known to have occurred. Within the context of REH theory, a paradox, first posed by Zuckerkandl, with respect to the high rate of covarion turnover and the nature of general function sites in proteins is resolved.
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  • 10
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    Monatshefte für Chemie 113 (1982), S. 275-284 
    ISSN: 1434-4475
    Keywords: Ferromagnetic properties ; Heusler alloys ; Magnetism ; Paramagnetic properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung QuarternäreHeusler-Legierungen der Zusammensetzung (Ni1−x Cu x )2MnSn wurden über den Konzentrationsbereichx=0–1 hergestellt. Alle waren einphasig und zeigten L 21-Struktur. Magnetische Messungen wurden sowohl im ferromagnetischen als auch im paramagnetischen Bereich durchgeführt. Sie ergaben für Mn, das einzige Atom das in dieser Art vonHeusler-Legierungen ein Moment zeigt, den üblichen Wert von ca. 4 µB und ein ihm entsprechendes μeff im paramagnetischen Bereich. Ein flaches Minimum derCurietemperatur kann mit Hilfe von di-dl-Wechselwirkung erklärt werden.
    Notes: Abstract QuarternaryHeusler alloys of composition (Ni1−x Cu x )2MnSn were prepared withx=0–1. They were all single phased with L21 structure. Alloys were investigated by magnetic measurements. For Mn, the only atom carrying a moment in this kind ofHeusler alloys, the usual value (∼4 µB) was found and a corresponding μeff in the paramagnetic region. A shallow minimum of theCurie temperatures can be explained on the basis of di-dl interaction.
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  • 11
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    Journal of biomedical science 1 (1994), S. 201-203 
    ISSN: 1423-0127
    Keywords: Hypertension ; Eicosanoid ; Rat ; Genetics ; Kidney
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The present paper reviews the evidence for a possible involvement of renal eicosanoids in the pathophysiology of high blood pressure in genetically hypertensive rats of the Lyon strain. Both in vivo and in vitro experiments suggest that an increased ability to synthesize the vasoconstrictor prostaglandin H2 and/or thromboxane A2 in renal vessels (1) acts as an autocrine amplifier of pressor agents and (2) may contribute to resetting the pressure natriuresis curve which is a prerequisite for the development and maintenance of hypertension.
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  • 12
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    Pure and applied geophysics 120 (1982), S. 375-388 
    ISSN: 1420-9136
    Keywords: Gravity ; Magnetism ; Scotland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract From our interpretation of the Bouguer gravity and aeromagnetic anomalies in south-east Scotland, we conclude that a massive granite batholith underlies the greater part of the eastern Southern Uplands. The granite model which we computed earlier from gravity anomalies in the Tweeddale area fits the observed magnetic anomalies closely, if a normal magnetization of 0.095 A m−1 is assigned, similar to values found for exposed local granites. Further gravity modelling shows that, apart from the Tweeddale boss, the granite shallows to less than 1 km near Lammer Law in East Lothian and extends north of the Lammermuir Fault. A model for the East Lothian volcanics was computed from their aeromagnetic anomalies, then their gravitational effect was combined with that estimated for the Devonian and Carboniferous sediments and the result stripped off the observed gravity field. The residual gravity anomalies were used to generate a two-dimensional model for the granite north of the Lammermuir Fault. The expected tectonic consequences of a massive granite batholith in the eastern Southern Uplands are compared with the known development of faults and sedimentary basins around its margins.
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  • 13
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    Pure and applied geophysics 118 (1980), S. 128-151 
    ISSN: 1420-9136
    Keywords: Galactic cosmic rays ; Solar proton events ; Particle precipitation ; Chemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract An assessment is made of the relative contribution of certain classes of energetic particle precipitation to the chemical composition of the middle atmosphere with emphasis placed on the production of odd nitrogen and odd hydrogen species and their subsequent role in the catalytic removal of ozone. Galactic cosmic radiation is an important source of odd nitrogen in the lower stratosphere but since the peak energy deposition occurs below the region where catalytic removal of O3 is most effective, it is questionable whether this mechanism is important in the overall terrestrial ozone budget. The precipitation of energetic solar protons can periodically produce dramatic enhancement in upper stratospheric NO. The long residence time of NO in this region of the atmosphere, where catalytic interaction with O3 is also most effective, mandates that this mechanism be included in future modelling of the global distribution of O3. Throughout the mesosphere the precipitation of energetic electrons from the outer radiation belt (60°≲Λ≲70°) can sporadically act as a major local source of odd hydrogen and odd nitrogen leading to observable O3 depletion. Future satellite studies should be directed at simultaneously measuring the precipitation flux and the concomitant atmosphere modification, and these results should be employed to develop more sophisticated models of this important coupling.
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  • 14
    ISSN: 1432-0827
    Keywords: Bacterial amphophile ; Purification ; Chemistry ; Resorption ; Ca influx ; Cyclic AMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The bone resorptive factor and amphipathic antigen (AcA) previously identified by us in preparations fromActinomyces viscosus have been partially purified, characterized chemically, and compared. They elute at the same location on chromatography with Ac 22. The fatty acid composition of AcA and the bone resorptive factor is the same. Some differences in carbohydrate composition are observed. TheActinomyces factor does not affect calcium influx or cyclic AMP in isolated bone cells. Therefore it is concluded that AcA stimulates resorption either by gaining entrance into bone cells or by way of a yet undetermined second messenger.
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  • 15
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    Oecologia 86 (1991), S. 243-250 
    ISSN: 1432-1939
    Keywords: Daphnia ; Life-history ; Genetics ; Variation ; Maturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Life-history traits of 101 clones from two populations of Daphnia magna were measured under controlled environmental conditions in the laboratory. Some individuals had four juvenile instars, others had five. This depended on their length at birth and on the population they came from. Females in the group with five juvenile instars were smaller at birth but larger and older at maturity than those with four juvenile instars. Within groups of females with equal numbers of preadult instars (instar groups) age and size at maturity increased with size at birth. This relationship differed significantly among instar groups for both age and size at maturity. Significant differences in age and size at maturity between two populations became non-significant when size at birth was used as a covariable in AN-COVA. Within populations, size at birth depended on the clone and on the parity of the clutch. First-clutch offspring were considerably smaller than those from later clutches. The results suggest that variability in life-history traits is common within and between clones, but that most of this variation can be accounted for by size at birth and the number of pre-adult instars.
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  • 16
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    Sexual plant reproduction 7 (1994), S. 290-296 
    ISSN: 1432-2145
    Keywords: Secale cereale ; Polyembryony ; Chromosome mosaics ; Rye ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have obtained one plant regenerated from rye tissue culture which showed a high percentage of polyembryonic seeds in its progeny. The mutation inducing the development of extra embryos is also influencing erroneous cell division, mitosis and meiosis. The genetic analysis indicated that the aptitude for polyembryonic seed formation is a heritable trait controlled by a dominant gene. However, for expression of the phenotype the female parent should have a specific cytoplasm.
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  • 17
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    Sexual plant reproduction 3 (1990), S. 31-34 
    ISSN: 1432-2145
    Keywords: Mucoraceae ; Zygomycetes ; Homothallic ; Genetics ; Nutritional complementation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Auxotrophic strains of Mucor genevensis and Zygorhynchus exponens were crossed and the resulting zygospores germinated. The presence of a true sexual cycle in both species was demonstrated by the recovery of recombinant genotypes. Expected Mendelian ratios were not realized, however. The presence of selfed zygospores among those isolated makes this observation understandable. It was possible to demonstrate nutritional complementation when young mating mycelium was transferred to minimal medium and forced heterokaryons were recovered.
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  • 18
    ISSN: 1432-0983
    Keywords: Protoplast ; Fusion ; Mitochondrial ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using a protoplast fusion technique we have been able to locate to the mitochondrial genome of the asporogenous yeast Torulopsis glabrata mutations conferring resistance to oligomycin, antimycin and diuron. When two strains differing in the size of their mtDNAs were fused the mitochondrial markers from the parent with the larger mtDNA (71–91) were transmitted predominantly among the fusion products. Both genetical and physical evidence support the occurrence of recombination in T. glabrata mitochondrial genome. Segregation of the mitochondrial genome appears to take place before the separation of the first bud from the fusion product.
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  • 19
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    Current genetics 4 (1981), S. 177-180 
    ISSN: 1432-0983
    Keywords: Genetics ; Yeasts, protoplasts ; Saccharomyces ; Hansenula
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasts of petites of strains 625-CI of Saccharomyces diastaticus and NCYC 1085 of Saccharomyces cerevisiae, originally obtained from the National Collection of Yeast Cultures, England, were fused with protoplasts of Candida pseudotropicalis, Saccharomyces rosei, Yaccharbmycesmontanus, Pichiamembranefaciens, Hansenula anomala, Hansenula capsulata, and Schizosac-charomyces pombe. The respiratory-competent products of the fusions were selected on the basis of using at least one of the carbon sources utilized by the petite parent and not by the other. The products of the fusion of C. pseudotropicalis x 1085(p−) consisted of two cell types; an oval cell which utilized both lactose and maltose and fermented lactose vigorously, and a cylindrical form which fermented maltose slowly. The S. rosei x 1085(p−) hybrids had acquired the ability to metabolize and ferment galactose, and to ferment maltose, from the petite parent. The P. membranaefaciens x 625(p−) hybrids acquired the ability to metabolize galactose, sucrose and maltose, but fermented only glucose, weakly, like the P. membranaefaciens parent strain. The H. capsulate x 625(p−) hybrids, unlike the hybrids with P. membranaefaciens or S. rosei, resembled the petite parent morphologically and also had the fermentative abilities of this strain (galactose, maltose, sucrose and starch), and the ability to ferment starch was considerably enhanced. The S. montanus x 625(p−) hybrids acquired the ability to utilize starch. Schizosaccharomyces pombe x 625(p−) hybrids resembled S. pombe morphologically, but had the ability to metabolize galactose and starch. Some of the asci produced by these hybrids contained abnormal numbers of spores. H. anomala x 624 x(p−) hybrids fermented starch, though weakly.
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  • 20
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    Journal of comparative physiology 166 (1990), S. 545-552 
    ISSN: 1432-1351
    Keywords: Honeybees ; Learning ; Classical conditioning ; Selection ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Four strains of the honeybee (Apis mellifera capensis), which were selected for high (N=2) or low (N=2) performance levels in classic conditioning of olfactory and mechanosensory stimuli, were examined in two instrumental visual learning tasks. Bees were trained to coloured cardboards either at the hive entrance or at the feeding station. Positive correlations were detected between olfactory/mechanosensory conditioning and visual learning. Good and poor learners from strains selected for olfactory conditioning differed significantly in their visual learning values. These strain differences reflect genetic differences in a common learning system rather than task specific differences in sensory, motor or motivational components. Parameters that were influenced by activity of the colony (duration of stay at the feeding place, time between visits) also differed among selected strains. These effects were not due to selection. Instead, they reflect a specific genetic background produced in each strain independently of selection. The results indicate that associative learning has a genetic basis which is independent of the sensory stimuli associated with reward, the learning procedure (classical conditioning or instrumental learning) or the motor patterns used to execute the learned behavior (proboscis extension, control for flight behavior, open field orientation).
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  • 21
    ISSN: 1432-072X
    Keywords: Carboxydotrophic bacteria ; Ribulosebis-phosphate carboxylase ; Phosphoribulokinase ; Hybridization ; Plasmids ; Genetics ; CO2 fixation ; Alcaligenes eutrophus ; Pseudomonas carboxydovorans ; Rhodospirillum rubrum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heterologous gene probes derived from cfxLp and cfxPp genes of Alcaligenes eutrophus H16 revealed the presence of structural genes encoding ribulosebisphosphate carboxylase (Rubisco) and phosphoribulokinase (PRK) on the genome of carboxydotrophic bacteria. The two genes were found to be rather conserved. In Pseudomonas carboxydovorans OM5 cfx genes reside on the plasmid pHCG3 and the chromosome as well, indicating that they are duplicated. Also in all plasmidharboring carboxydotrophic bacteria cfxL and cfxP structural genes were found to be plasmid-coded. Our results extend the list of carboxydotrophy structural genes residing on the plasmid pHCG3 and strongly support the idea that the components essential for the chemolithoautotrophic utilization of CO by Pseudomonas carboxydovorans OM5 are plasmid-coded. A cfxL gene probe from Rhodospirillum rubrum did not detectably hybridize with DNA from any of the carboxydotrophic bacteria examined.
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  • 22
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    Astrophysics and space science 216 (1994), S. 153-154 
    ISSN: 1572-946X
    Keywords: Chemistry ; Molecular Hydrogen ; Cloud Models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Observations of the CO J=1-0 line are commonly used as a tracer for molecular material in clouds. The ratio of the H2 column density to the integrated intensity of this line,X, is often taken to be constant, despite theoretical and observational uncertainty. We have tried to identify how this ratio depends on cloud parameters, testing a simple theoretical argument suggesting its invariance with respect to density. The apparent constancy can be understood if clouds are clumpy on scales of Av ≈ 1-2 mag.
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    Journal of mathematical biology 15 (1982), S. 65-77 
    ISSN: 1432-1416
    Keywords: Epidemics ; Genetics ; Continuous models
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    Topics: Biology , Mathematics
    Notes: Abstract Continuous deterministic models are used to investigate the relationship between the epidemiology of endemic infectious disease and the genetics of natural selection in the host population when a specific genetic locus controls susceptibility to disease under a variety of circumstances. One locus, two allele genes are considered in the contexts of haploid and diploid host populations while the agent of infection is assumed to be invariant. It is found that polymorphic equilibria exist and are stable for certain parameter combinations in each of the cases studied. The equilibrium levels of gene frequencies and disease prevalence depend on both genetic and epidemic factors.
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    Journal of mathematical biology 17 (1983), S. 289-304 
    ISSN: 1432-1416
    Keywords: Endemicity ; Epidemics ; Genetics ; Deterministic models ; Stability
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    Topics: Biology , Mathematics
    Notes: Abstract A discrete time genetics model is developed for populations that are undergoing selection due to infectious disease. It is assumed that the generation time of the host and infectious agent are non-synchronous and that only the host population is evolving. Two classes of epidemic processes are considered. The first class is for infectious agents that confer immunity following infection, while the second class is for those that do not confer immunity. The necessary and sufficient conditions are found in order for the disease to persist in a stable polymorphic host population. These conditions are shown to depend on the density of susceptibles, the selection coefficients, and the severity and class of the disease process.
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  • 25
    ISSN: 1432-1343
    Keywords: Analysis of variance ; Choropleth map ; Ecology ; Genetics ; Geography ; Permutation test ; Spatial autocorrelation
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    Topics: Mathematics
    Description / Table of Contents: Résumé Cet article présente une solution au problème de l'analyse de variance, pour certains cas où la variable à analyser est spatialement autocorr élée alors que le critère de classification représente des sous-régions connexes du territoire à l'étude. On sait que les méthodes classiques d'analyse de variance ne sont pas applicables dans ce type de situation puisque la condition d'indépendance des échantillons n'est pas respectée; l'autocorrélation positive réduit la variabilité intragroupe, si bien que la quantité relative de variabilité intergroupe s'en trouve artificiellement augmentée. Cette situation correspond en réalité à une vaste catégorie de problèmes en génétique des populations, en écologie et dans d'autres branches de la biologie, ainsi qu'en épidémiologie, en géographie, en géologie, en science économique, en science politique et en sociologie. Ce nouveau test appartient à la famille des tests par permutation. Nous calculons la somme des dispersions intragroupes et testons contre une distribution de référence obtenue en permutant les régions géographiques un grand nombre de fois sur la carte. La véritable difficulté de ce test est d'ordre algorithmique, puisqu'il n'est pas facile de permuter des régions sur une carte, de façon à ce que chaque groupe demeure connexe, et que la carte permutée occupe le même espace total que la carte d'origine. Cet article présente la théorie, les algorithmes, ainsi que des résultats obtenus par cette méthode. Un programme écrit en PASCAL est disponible.
    Notes: Abstract The classical method for analysis of variance of data divided in geographic regions is impaired if the data are spatially autocorrelated within regions, because the condition of independence of the observations is not met. Positive autocorrelation reduces within-group variability, thus artificially increasing the relative amount of among-group variance. Negative autocorrelation may produce the opposite effect. This difficulty can be viewed as a loss of an unknown number of degrees of freedom. Such problems can be found in population genetics, in ecology and in other branches of biology, as well as in economics, epidemiology, geography, geology, marketing, political science, and sociology. A computer-intensive method has been developed to overcome this problem in certain cases. It is based on the computation of pooled within-group sums of squares for sampled permutations of internally connected areas on a map. The paper presents the theory, the algorithms, and results obtained using this method. A computer program, written in PASCAL, is available.
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  • 26
    ISSN: 1432-2242
    Keywords: Triticum aestivum L. ; Doubled-haploids ; Genetics ; Androgenesis
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    Topics: Biology
    Notes: Summary Plants of three common wheat (Triticum aestivum L. em. Thell) cultivars and one randomly selected doubled-haploid line derived by anther culture from each of the three cultivars were each grown in three environments, a field environment, a greenhouse environment, and a growth chamber environment. Anthers containing largely miduninucleate to late uninucleate microspores were cultured and calli were induced to regenerate plants in order to assess the effects of cultivar, cultivar family (cultivar and corresponding doubled-haploid derivative), anther-donor plant environment, and cultivar X environment interaction on androgenic responses. Large differences in response were observed among cultivars as well as between cultivars and doubled-haploids. Differences between cultivar and doubled-haploid within cultivar family usually resulted from higher frequency of response in the cultivar, contrary to the hypothesis that anther culture per se constitutes a general selective device for superior androgenic responses. Also, in a second experiment, anther callusing frequency was greater in the cultivar ‘Kitt’ than in any of five unique doubled-haploid lines derived from ‘Kitt’. Significant effects were also observed in the first experiment for the interactions of cultivar family X environment as well as doubled-haploid vs. cultivar X environment, although the effect of environment itself was less significant than these interactions.
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    Theoretical and applied genetics 67 (1984), S. 97-111 
    ISSN: 1432-2242
    Keywords: Genetics ; Nitrogen ; Grain crops ; Selection ; Plant breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary It is necessary to increase protein productivity of grain crops to meet present and future world protein requirements. Conventional plant breeding methodology has been to select genotypes with enhanced yield or grain protein concentration. In addition to this determination of end product, we suggest measurements of a number of physiological and biochemical processes of nitrogen (N) metabolism which precede plant maturity as selection criteria for enhanced N metabolism and grain crop productivity. The measurement across the growing season of genotypic variation in components of N metabolism would constitute a physiological/biochemical selection program to be incorporated with the determination of harvestable end product. A properly designed physiological/biochemical selection program would integrate the effects of plant genotype, environment, and their interactions allowing identification of the factors limiting productivity of particular genotypes, and would also estimate end product. Our review of literature pertinent to whole plant N metabolism suggests that such a selection program initially include NO 3 - uptake, N2 fixation, N accumulation, N remobilization, seed protein synthesis, and Nitrogen Harvest Index.
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    Theoretical and applied genetics 69 (1984), S. 15-21 
    ISSN: 1432-2242
    Keywords: Genetics ; Heat tolerance ; Thyroid function ; Chickens
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    Topics: Biology
    Notes: Summary Five experiments were conducted to assess the genetic variation in thyroid function (T3, T4), body weight and heat stress survival time in chickens. Thyroxine (T4) levels were found to be elevated in response to 4 and 8 μg bovine thyroid stimulating hormone (TSH) in experiment I. In experiment II, 4 μg of TSH was injected into chickens from 30 sire families of the Athens-Canadian Randombred population. The heritability of T4 levels after TSH injection was high. In experiment III, families identified as having innate high or low T4 levels after TSH injection and a group of control birds were subjected to a heat Stressor of 50 °C for up to 240 min at six weeks of age and heat stress survival time was studied. The groups did not differ from each other in heat stress survival time. Experiment IV was similar to experiment I except triiodothyronine (T3) was also measured after TSH injection. Both T4 and T3 levels after TSH injection were moderately heritable. In experiment V birds were reared to six weeks of age and heritability calculated for body weight, T4, T3, and heat stress survival time. Heritabilities were high for body weight, moderate for T4 and heat stress survival time, and low for T3. Phenotypic correlations were significant and negative for heat stress survival time with body weight and T4, and for body weight with T3 after TSH. Significant positive correlations were found for T4 with T3 after TSH and also T4 and body weight. Analysis of genetic correlations suggested that none of the traits studied would be an adequate selection parameter for achieving heat tolerance without reducing body weight.
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    ISSN: 1432-2242
    Keywords: Genetics ; Symbiosis ; Nitrogen fixation ; Coevolution
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    Topics: Biology
    Notes: Summary To determine the relationship between nodulation restriction by the Rj4 allele of soybean, rhizobitoxine-induced chlorosis, and taxonomic grouping of bradyrhizobia, 119 bradyrhizobial isolates were tested in Leonard jar culture for nodulation response and chlorosis induction. In addition to strain USDA 61, the strain originally reported as defining the Rj4 response, eight other isolates (i.e., USDA 62, 83, 94, 238, 252, 259, 260, and 340) were discovered to elicit the nodulation interdiction of the Rj4 allele. Only 16% of all the bradyrhizobial strains tested induced chlorosis, but seven of the nine strains (78%) interdicted by the Rj4 allele were chlorosis-inducing strains. Furthermore, in tests for antibiotic resistance profile, eight of the nine interdicted strains (89%) were classed in DNA homology group II. This evidence suggests that the Rj4 allele has a positive value to the host plant in shielding it from nodulation by certain chlorosis-inducing bradyrhizobia of a DNA homology group with impaired efficiency of nitrogen fixation with soybean.
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    Theoretical and applied genetics 83 (1991), S. 24-32 
    ISSN: 1432-2242
    Keywords: Genetics ; Growth curve ; Body weight ; Chickens
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    Topics: Biology
    Notes: Summary Genetic improvement in growth of poultry has traditionally proceeded via selection for body weight at a fixed age. Due to increased maintenance costs and reproductive problems of adult broiler breeders, the potential for genetic manipulation of the growth curve has been receiving increased interest. Research of both male and female progeny of a three-way diallel cross was used to investigate the inheritance of growth curve parameters. The Laird form of the Gompertz equation was used to determine growth curve parameters, and was suited to the juvenile growth data frequently collected from meat-type chickens. Growth rate exhibited significant heterosis due to both autosomes and the sex chromosomes. Age at inflection point also exhibited significant average heterosis, though only among females. Growth rate was also influenced by average line effects, as was age at inflection point. Maternal effects had no influence on growth curve parameters, while additive sex linkage was observed for growth rate. Phenotypic and genetic correlations were calculated among the growth curve parameters and suggest that specific breeding programs could alter the growth trajectory of the contemporary broiler chicken. Moderate heritabilities were observed for the growth curve parameters and support the hypothesis that the growth curve could be altered via genetic manipulation of early postnatal growth, especially during the first 14 days post-hatch.
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  • 31
    ISSN: 1432-2242
    Keywords: Maize ; Restriction fragment length polymorphisms (RFLPs) ; Qualitative and quantitative inheritance ; Plant breeding ; Genetics
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    Notes: Abstract Restriction fragment length polymorphisms (RFLPs) and one morphological marker were used to investigate quantitative trait loci (QTL) for morphological and physiological traits evaluated on 150 F2∶3 maize (Zea mays L.) lines derived from the cross of elite U.S. Corn Belt inbreds Mo17 and H99. F2∶3 lines were grown in a replicated experiment and evaluated for plant and ear heights and flowering traits. QTL were identified for each trait, and genetic effects were determined. Estimated gene action for the flowering traits was predominantly overdominance. Both parents contributed toward increased values for anthesis and silk emergence. QTL for increased plant and ear heights were usually contributed by the taller parent, Mo17. Estimated gene action for these traits was mainly partial to overdominance. QTL for plant height were located in the vicinity of loci defined by alleles with qualitative effects on plant height.
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    Theoretical and applied genetics 89 (1994), S. 959-963 
    ISSN: 1432-2242
    Keywords: Sugarcane ; Polyploidy ; Genetics ; Evolution ; Breeding ; DNA markers ; Arbitrarily primed PCR ; RAPD markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Recent work has revealed random chromosome pairing and assortment in Saccharum spontaneum L., the most widely distributed, and morphologically and cytologically variable of the species of Saccharum. This conclusion was based on the analysis of a segregating population from across between S. spontaneum ‘SES 208’ and a spontaneously-doubled haploid of itself, derived from anther culture. To determine whether polysomic inheritance is common in Saccharum and whether it is observed in a typical biparental cross, we studied chromosome pairing and assortment in 44 progeny of a cross between euploid, meiotically regular, 2n=80 forms of Saccharum officinarum ‘LA Purple’ and Saccharum robustum ‘ Mol 5829’. Papuan 2n=80 forms of S. robustum have been suggested as the immediate progenitor species for cultivated sugarcane (S. officinarum). A total of 738 loci in LA Purple and 720 loci in Mol 5829 were amplified and typed in the progeny by arbitrarily primed PCR using 45 primers. Fifty and 33 single-dose polymorphisms were identified in the S. officinarum and S. robustum genomes, respectively (χ 2 at 98%). Linkage analysis of single-dose polymorphisms in both genomes revealed linkages in repulsion and coupling phases. In the S. officinarum genome, a map hypothesis gave 7 linkage groups with 17 linked and 33 unlinked markers. Four of 13 pairwise linkages were in repulsion phase and 9 were in coupling phase. In the S. robustum genome, a map hypothesis gave 5 linkage groups, defined by 12 markers, with 21 markers unlinked, and 2 of 9 pairwise linkages were in repulsion phase. Therefore, complete polysomic inheritance was not observed in either species, suggesting that chromosomal behavior is different from that observed by linkage analysis of over 500 markers in the S. spontaneum map. Implications of this finding for evolution and breeding are discussed.
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    Theoretical and applied genetics 89 (1994), S. 313-317 
    ISSN: 1432-2242
    Keywords: Genetics ; Rice ; Phosphorousefficiency ; Diallel analysis
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    Topics: Biology
    Notes: Abstract The inheritance of phosphorous (P) — deficiency tolerance in rice was investigated by a sevenparent diallel. The parent materials involved were four P-efficient (IR20, IR54, IR28, and Mahsuri), one moderately P-efficient (TN1), and two P-inefficient (IR31406333-1 and IR34686-179-1-2-1), genotypes. Relative tilering ability (RTA) under P-deficient and P-supplemented soil conditions was the parameter used in determining the tolerance level of the different genotypes. Diallel graph analysis revealed that tolerant parents have an excess of recessive genes, while moderate and susceptible parents possess more dominant genes. Genetic-component analysis suggested that both additive and dominance gene effects are involved in the inheritance of P-deficiency tolerance in rice. The trait exhibited over doiminance as confirmed by the graphical analysis. Narrow-sense heritability of the trait was moderate (0.50) and environmental effects were low. Both the general combining ability (GCA) and the specific combining ability (SCA) were significant, but GCA was more prevalent than SCA. Tolerant parents exhibited a high GCA whereas susceptibles have a very poor GCA, suggesting that tolerant parents were mostly enriched in additive genes and susceptible parents in non-additive genes. Crosses involving two high general combiners showed low SCA effects whereas crosses between poor general combiners manifested highly-significant SCA values.
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    Theoretical and applied genetics 67 (1983), S. 67-73 
    ISSN: 1432-2242
    Keywords: Somaclonal variation ; Genetics ; Oryza sativa L. ; Rice improvement
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    Topics: Biology
    Notes: Summary The inheritance and variations of some traits of more than 2,000 somatic cell derived plants of rice (Oryza sativa L.) were investigated in the second and third generations (T2 and T3) of regenerated plants (somaclones). The percentages of multiploids occurring in somaclones ranged from 0–13.3 in nine varieties (or hybrids) of ‘Hsien’ (indica) group, but no multiploid was found in nine varieties (or hybrid) of ‘Keng’ (japonica) group. A dwarf mutant with the height of only 20 cm was isolated in the T2 of ‘Tai-Zhong-Yu 39’. Genetic analysis indicated that it was controlled by a single recessive gene. The frequencies of chlorophyll mutations probably controlled by cytoplasmic genes decreased gradually with the advance of generations. The variations of five quantitative traits — plant height, grain weight, etc. — in 950 T2 pedigree lines of four varieties were also studied. Only 24.4% of the lines were normal in all the traits studied. Variation frequencies of different traits were from 11.5% to 39.5%. And there was an obvious tendency for the plant height to become shorter, number of productive tillers to increase and 1,000 grain weight to be lighter, whatever the variety studied. Traits were uniform within each of more than 90% of all T2 lines studied. What is more interesting, variations phenotyped in T2 proved to breed true. Causes of somaclonal variations are discussed, as well as their potentials in breeding.
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    Theoretical and applied genetics 82 (1991), S. 761-764 
    ISSN: 1432-2242
    Keywords: Rye ; Male sterility ; Genetics ; Gene location ; Trisomies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genetics and relationships between the genes in rye located in the nucleus and cytoplasm of the male sterility of the G-type were investigated. A factor inducing male sterility was found in the cytoplasms or rye cv Schlägler alt and rye cv Norddeutscher Champagner. Monogenic inheritance was observed in linkage tests. Using primary trisomies of rye cv Esto, the nuclear gene ms1 was found to be located on chromosome 4R. Modifying genes, probably masked in normal cytoplasm but expressed in male-sterility-inducing cytoplasm together with gene ms1, were located on chromosomes 3R (ms2) and 6R (ms3). Mono-, di-, and trigenic inheritance types were found in backcross progenies of trisomies.
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    Theoretical and applied genetics 86 (1993), S. 333-338 
    ISSN: 1432-2242
    Keywords: Genetics ; Rice ; Salinity ; Tolerance ; Na-Kratio ; Diallel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genetics of salinity tolerance in rice was investigated by a nine-parent complete diallel including reciprocals. Test materials involved susceptible (IR28, IR29, and MI-48), moderately tolerant (IR4595-4-1-13, IR9884-54-3-1E-P1, and IR10206-29-2-1), and tolerant (“Nona Bokra”, “Pokkali”, and SR26B) parents. Twoweek-old seedlings were grown in a salinized (EC = 12 dS/m) culture solution for 19 days under controlled conditions in the IRRI phytotron. Typical characteristics of salinity tolerance in rice were found to be Na+ exclusion and an increased absorption of K+ to maintain a good Na-K balance in the shoot. Genetic component analysis (GCA) revealed that a low Na-K ratio is governed by both additive and dominance gene effects. The trait exhibited overdominance, and two groups of genes were detected. Environmental effects were large, and the heritability of the trait was low. Our findings suggest that when breeding for salt tolerance, selection must be done in a later generation and under controlled conditions in order to minimize environmental effects. Modified bulk and single-seed descent would be the suitable breeding methods. Combining ability analysis revealed that both GCA and specific combining ability (SCA) effects were important in the genetics of salt tolerance. Moderately tolerant parents — e.g., IR4595-4-1-13 and IR9884-54-3-1E-P1 — were the best general combiners. Most of the best combinations had susceptible parents crossed either to moderate or tolerant parents. The presence of reciprocal effects among crosses necessitates the use of susceptible parents as males in hybridization programs. Large heterotic effects suggest the potential of hybrid rice for salt-affected lands.
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    Theoretical and applied genetics 88 (1994), S. 754-758 
    ISSN: 1432-2242
    Keywords: Potato breeding ; Potato leaf roll virus ; Virus resistance ; Major gene resistance ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The concentration of potato leaf roll virus (PLRV), as measured by a quantitative enzyme-linked immunosorbent assay, in the foliage of potato plants (Solanum tuberosum) of cv ‘Maris Piper’ with secondary infection was 2900 ng/g leaf, whereas in clones G7445(1) and G7032(5) it was 180 ng/g leaf and 120 ng/g leaf, respectively. To examine the genetic control of resistance to PLRV multiplication, reciprocal crosses were made between the susceptible cultivar ‘Maris Piper’ and the two resistant clones, and the three parents were selfed. Seedling progenies of these families were grown to generate tubers of individual genotypes (clones). Clonally propagated plants were graft-inoculated, and their daughter tubers were collected and used to grow plants with secondary infection in which PLRV concentration was estimated. The expression of resistance to PLRV multiplication had a bimodal distribution in progenies from crosses between ‘Maris Piper’ and either resistant clone, and also in progeny from selfing the resistant parents, with genotypes segregating into high and low virus titre groups. Only the progeny obtained from selfing ‘Maris Piper’ did not segregate, all genotypes being susceptible to PLRV multiplication. The pattern of segregation obtained from these progenies fits more closely with the genetical hypothesis that resistance to PLRV multiplication is controlled by two unlinked dominant complementary genes, both of which are required for resistance, than with the simpler hypothesis that resistance is conferred by a single dominant gene, as published previously.
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    Theoretical and applied genetics 57 (1980), S. 91-95 
    ISSN: 1432-2242
    Keywords: Cell culture ; Picloram-tolerance ; Genetics ; Uptake studies ; Nicotiana tabacum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A genetic and preliminary biochemical analysis has been performed on four picloram-tolerant mutants of Nicotiana tabacum that were isolated from cell cultures. The four mutations define three distinct linkage groups. Mutant seedlings incorporate radioactively labeled picloram normally and do not modify or degrade the herbicide in a manner that alters its solubility characteristics.
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  • 39
    ISSN: 1432-2242
    Keywords: Genetics ; Loci ; Powdery mildew ; Prolamin ; Recombination
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    Notes: Summary The linkage relationship among the loci Hor1, Hor2, Ml-k and Ml-a on the short arm of chromosome 5 was studied by progeny testing the F2 generation of two crosses. The loci Hor1 and Hor2 code for polypeptides of the storage protein hordein (prolamin) and the loci Ml-k and Ml-a determine the resistance reaction with some powdery mildew fungi cultures. The order of the loci is Ml-k, Hor1, Ml-a, and Hor2, the first named being nearest the centromere. The recombination percentage between Hor1 and Hor2 was determined in the F1 and F2 generations in both crosses, the combined estimate being 7.4±0.9 per cent. The recombination percentage estimated between Ml-k and Hor1 was 4.0±1.3, between Hor1 and Ml-a, 5.3±1.1, and between Ml-a and Hor2, 6.1±1.2. The estimates involving the Ml- loci were all probably a little too high.
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    Theoretical and applied genetics 60 (1981), S. 229-236 
    ISSN: 1432-2242
    Keywords: Glutenin ; Triticum ; Genetics ; SDS ; Polyacrylamide-gel-electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The high-molecular-weight (HMW) subunits of glutenin from about 185 varieties were fractionated by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). About 20 different, major subunits were distinguished by this technique although each variety contained, with only a few exceptions, between 3 and 5 subunits. Further inter-varietal substitution lines to those already described (Payne et al. 1980) were analysed and the results indicate that all the HMW subunits are controlled by the homoeologous group 1 chromosomes. All hexaploid varieties studied except ‘NapHal’ contained two major subunits controlled by chromosome 1D. Their genes were shown to be tightly linked genetically for only four different types of banding patterns were observed. The nominal molecular weights determined after fractionation in 10% polyacrylamide gels were between 110,000 and 115,000 for the larger of the two subunits and between 82,000 and 84,000 for the smaller. One quarter of the varieties contained only one major HMW subunit controlled by chromosome 1B whereas the rest had two. The chromosome 1B subunits were the most varied and nine different banding patterns were detected. All the subunits had mobilities which were intermediate between those of the two chromosome 1D-controlled subunits. Only two types of HMW subunit controlled by chromosome 1A were detected in all the varieties examined; a single variety never contained both of these subunits and 40% of varieties contained neither. The chromosome 1A-controlled subunits had slightly slower mobilities in 10% gels than the largest HMW subunit controlled by chromosome 1D. About 100 single grains were analysed from each of five different crosses of the type (F1 of variety A × variety B) × variety C. The results indicate that the genes on chromosome 1B which control the synthesis of subunits 6, 7, 13, 14 and 17 are allelic, as are the genes of the chromosome 1A-controlled subunits, 1 and 2.
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    Theoretical and applied genetics 60 (1981), S. 265-268 
    ISSN: 1432-2242
    Keywords: Agrocybe ; Genetics ; Fruiting ; Biomass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In the edible white rot fungus Agrocybe aegerita the threshold from mycelial growth to fruit body formation is under control of a single gene in both monokaryons and dikaryons. The allele su opens the pathway for fruiting and allows the subsequent expression of the fruiter genes fi+ (fruit body initials) and fb + (fruit bodies). Its allele, su +, suppresses monokaryotic fruiting completely and restricts dikaryotic fruiting drastically. The detection of this threshold gene su +/su and its action and interactions has practical implication in that an opportunity for concerted breeding is created. First results indicate that the fruiter genes are involved in two essential parameters of productivity. Both time of fruiting and biomass production depend on the two fruiter genes fi + and fb +. Comparable results obtained with two other basidiomycetes suggest that the genetic control of fruiting in Agrocybe aegerita is a general mechanism which may be made use of in breeding work with other basidiomycetes of economic value.
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    Theoretical and applied genetics 62 (1982), S. 97-102 
    ISSN: 1432-2242
    Keywords: Guide dogs ; Behaviour ; Genetics
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    Topics: Biology
    Notes: Summary Many dogs are found to be unsuitable for training as guide dogs for the blind. Consequently the Royal Guide Dogs for the Blind Association of Australia has embarked on a breeding program to produce a strain of labrador dogs which is suitable for guide dog training. The most common reasons for rejecting dogs are fearfulness, dog distraction, excitability, health and physical reasons and hip dysplasia. The selection program seems to have been successful in improving the success rate mainly by lowering fearfulness, but there has not been a continuing improvement. This is probably due to continual introduction of dogs from other populations into the breeding program. Males suffer from a higher rejection rate due to dog distraction and a lower rejection rate due to fearfulness and excitability than females, so that there is little sex difference in overall success rate. The heritability of success (0.44) is high enough to predict further progress from selection, again mainly against fearfulness. Variation in environment prior to 6 weeks of age, in age when dogs were placed into a private home and in age when males were castrated, had little effect on the success rate.
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    Theoretical and applied genetics 62 (1982), S. 233-239 
    ISSN: 1432-2242
    Keywords: Hordeum vulgare ; Haploids ; Androgenetic responsiveness ; Genetics
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    Topics: Biology
    Notes: Summary Anthers of 55 different spring barley (Hordeum vulgare) hybrids and four varieties were cultured in vitro. Microspores of each hybrid gave rise to calluses and subsequently plantlets, from all hybrids, except one. As criteria of microspore responsiveness, callus formation and plant regeneration frequencies were studied in detail. Large differences with regard to these criteria were found, which were traced back to the genotype of the anther donor plant. Callus formation varied between 3.3 and 73.2 per 1,000 anthers plated, whereas green plant regeneration ranged from 0 to 12.7 per 1,000 anthers cultured. Comparisons of microspore regeneration frequencies of hybrids and their parents indicated that culture responsiveness is a heritable, complex character involving at least two different and separately inherited mechanisms: 1) the ability of microspores within anthers to divide and give rise to calluses and subsequently 2) the ability of calluses for morphogenesis, to yield green or albino plants. Because it is heritable, anther culture responsiveness can be transferred to breeding material which is initially non-responsive. This genetic way of improving success in androgenetic haploid production appears to be more realistic than the search for optimum culture conditions.
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    Theoretical and applied genetics 67 (1983), S. 53-58 
    ISSN: 1432-2242
    Keywords: Aegilops umbellulata ; Genetics ; Lectin ; Triticum aestivum
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    Topics: Biology
    Notes: Summary Each of the three genomes in hexaploid wheat controls the expression of a specific lectin in the embryo. The chromosomes which control their synthesis were determined using nullisomic-tetrasomic and inter-varietal chromosome substitution lines of ‘Chinese Spring’. All three wheat lectins were shown to be controlled by the homoeologous group 1 chromosomes. Using ditelosomic lines of ‘Chinese Spring’ the lectin genes could be localized on the long arms of chromosomes 1A and 1D. Inter-specific addition and substitution lines of Aegilops umbellulata chromosomes to ‘Chinese Spring’ indicated that chromosome 1U, which is homoeologous to the group 1 chromosomes of wheat, controls lectin synthesis.
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    Theoretical and applied genetics 56 (1980), S. 5-9 
    ISSN: 1432-2242
    Keywords: Genetics ; Poultry ; Family selection ; Individual selection
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    Topics: Biology
    Notes: Summary Responses to single trait selection on individual phenotype and sire-family mean phenotype for survivor's egg weight and rate of lay were measured for a single generation in 13 replicates. Each replicate-selection criterion-trait subclass consisted of eight sire families or 72 females measured and was reproduced from the best 25% of the families or individuals. The realized heritability of egg weight was 0.39 and that of rate of lay was 0.31, both of which were significantly greater than zero but not significantly different from the predicted values based on halfsib correlations in the base population. The standardized response to sire-family selection was less than the response to individual selection for both traits and the difference was significant for rate of lay (0.10; 0.31) but not for egg weight (0.22; 0.39). The predicted responses to sire-family selection were less than those for individual selection for both traits, and the observed responses to sire-family selection were not significantly different from the predicted values for either trait. These experimental results do not disagree with the theoretical expectations of the relative efficiencies of individual and sire-family selection.
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    Theoretical and applied genetics 58 (1980), S. 113-120 
    ISSN: 1432-2242
    Keywords: Glutenin ; Triticum ; Genetics ; SDS-polyacrylamide ; Gel-electrophoresis
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    Topics: Biology
    Notes: Summary The electrophoretic mobilities of the high-molecular-weight (HMW) subunits of glutenin from 7 varieties were compared by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate (SDS). In total, 12 subunits were clearly resolved and they had nominal molecular weights of between 95,000 and 140,000. The chromosomes which control their synthesis were determined using monosomic lines and inter-varietal substitution lines. All subunits were shown to be controlled by the homoeologous group 1 chromosomes. Each variety contains between 3 and 5 HMW subunits; two are under the control of the 1D chromosome, 1 or 2 are controlled by chromosome 1B and 0 or 1 by chromosome 1A. The segregation of two 1D-controlled subunits of similar electrophoretic mobilities were analysed in the F2 progeny of crosses between ‘Chinese Spring’ and ‘Holdfast’. The results suggest that the genes which code for the two proteins are allelic.
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  • 47
    ISSN: 1432-2242
    Keywords: Beta vulgaris ; Sugar beet ; Isozymes ; Genetics ; Linkage ; Pollen fertility restorer
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    Topics: Biology
    Notes: Summary The NADP-specific malate dehydrogenase isozymes were controlled by multiple gene systems. Three genes coding for dimeric enzymes segregated in a dependent fashion (NADP-Mdh 1, NADP-Mdh 2, NADP-Mdh 3). A fourth gene (NADP-Mdh 4), also coded for dimers, but was not polymorphic in B. vulgaris. A fifth gene (NADP-Me 1) coded for enzymes active as monomers. Two genes were found to control the main zone of NAD-specific malate dehydrogenase: one coded for dimers (Mdh 1), while a second (Mdh 2) was not polymorphic in the assessions studied. 6-P-Gluconate dehydrogenase was not polymorphic in B. vulgaris; the two types detected on SGE1 electrophoresis were due to developmental expression of the different systems. No genetical segregations could be detected in progeny of crosses of the distinct phenotypes. A shikimate dehydrogenase gene (Skdh 1) that coded for monomers was identified. The diaphorase system was rather complex, but one gene (Dia 1) coding for monomeric enzymes could be identified. Aconitase was found to be controlled by two independent genes (Aco 1, Aco 2), both polymorphic and coding for proteins active as monomers. Tight linkage was found between the genes NADP-Mdh 1, NADP-Mdh 2 and NADP-Mdh 3. Linkage was also found between a pollen fertility restorer (Z) and the Mdh 1 gene. The identification of linkage with Aco 1 needs further investigation. R segregated independently from Mdh 1, Aco 1 and Dia 1. Independent segregations were scored for isozyme genes Pgm 2, Icd 1, Ak 1, Gpi 1, Aco 1 and Dia 1.
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    Theoretical and applied genetics 81 (1991), S. 50-58 
    ISSN: 1432-2242
    Keywords: Vicia faba ; Legumin ; Vicilin ; Structure ; Genetics
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    Topics: Biology
    Notes: Summary Legumin and vicilin were purified from seeds of Vicia faba L. var. Scuro, characterized in different electrophoretic systems, and used to produce polyclonal antibodies in rabbits. Two-dimensional electrophoretic studies showed a wide range of heterogeneity in the subunits of both legumin and vicilin. Legumin was found to be composed of 29 disulphide-linked subunit pairs with different molecular weight and/or isoelectric point. Western blot analysis of legumin of several mutants revealed molecular polymorphism based on a corresponding gene family. Three different α-major legumin patterns were found, and inheritance studies showed that the 34.3-kD legumin polypeptide is the product of one locus, Lg-1α, which is the first legumin genetic locus described in Vicia faba. Vicilin was found to be composed of as many as 59 subunits distributed in a molecular weight range of 65.7 to 42.8 kD (major polypeptides) and 37.2 to 15.2 kD (minor polypeptides), with different isoelectric points. A model is proposed that explains the possible formation of the minor subunits and the major subunits of 48.2 and 46 kD molecular weight (MW) from proteolytic cleavages and/or glycosilation of precursor polypeptides. Ten different vicilin electrophoretic patterns were observed among the analyzed accessions, which showed large molecular polymorphism that proved to be under genetic control.
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    Theoretical and applied genetics 82 (1991), S. 771-776 
    ISSN: 1432-2242
    Keywords: Secale cereale ; RFLP ; α-Amylase ; Genetics ; Isozymes
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    Topics: Biology
    Notes: Summary Rye α-Amy1, α-Amy2, and α-Amy3 genes were studied in the cross between inbred lines using wheat α-amylase cDNA probes. The α-Amy1 and α-Amy2 probes uncovered considerable restriction fragment length polymorphism, whereas the α-Amy3 region was much more conserved. The numbers of restriction fragments found and the F2 segregation data suggest that there are three α-Amy1 genes, two or three α-Amy2 genes, and three α-Amy3 genes in rye. These conclusions were supported by a simultaneous study of α-amylase isozyme polymorphism. The F2 data showed the three individual α-Amy1 genes to span a distance of 3cM at the locus on chromosome 6RL. The genes were mapped relative to other RFLP markers on 6RL. On chromosome 7RL two α-Amy2 genes were shown to be separated by 5 cM. Linkage data within α-Amy3 on 5RL were not obtained since RFLP could be detected at only one of the genes.
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    Theoretical and applied genetics 84 (1992), S. 714-719 
    ISSN: 1432-2242
    Keywords: Wheat ; Salinity ; Genetics
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    Topics: Biology
    Notes: Summary Accessions of Triticum tauschii (Coss.) Schmal. (D genome donor to hexaploid wheat) vary in salt tolerance and in the rate that Na+ accumulates in leaves. The aim of this study was to determine whether these differences in salt tolerance and leaf Na+ concentration would be expressed in hexaploid wheat. Synthetic hexaploids were produced from five T. tauschii accessions varying in salt tolerance and two salt-sensitive T. turgidum cultivars. The degree of salt tolerance of the hexaploids was evaluated as the grain yield per plant in 150 mol m-3 NaCl relative to grain yield in 1 mol m-3 NaCl (control). Sodium concentration in leaf 5 was measured after the leaf was fully expanded. The salt tolerance of the genotypes correlated negatively with the concentration of Na+ in leaf 5. The salt tolerance of the synthetic hexaploids was greater than the tetraploid parents primarily due to the maintenance of kernel weight under saline conditions. Synthetic hexaploids varied in salt tolerance with the source of their D genome which demonstrates that genes for salt tolerance from the diploid are expressed at the hexaploid level.
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  • 51
    ISSN: 1432-2242
    Keywords: Genetics ; Disease ; Mapping ; Breeding
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    Topics: Biology
    Notes: Abstract Molecular markers at 103 loci were used to identify the location of quantitative sources of resistance to Exserohilum turcicum in 150 F2∶3 lines of a B52/Mo17 maize population. Host-plant response was measured in terms of the average number of lesions per leaf, the average percent leaf tissue diseased (severity), and the average size of lesions. The location of quantitative trait loci were compared with three loci having known qualitative effects, namely Ht1, Ht2 and bx1. Chromosomal regions containing the Ht1 and Ht2 loci showed a small contribution in determining lesion size, even though alleles with dominant, qualitative effects at these loci have never been reported in either inbred parent. Similar effects were not observed for the number of lesions or for disease severity. Likewise, some contribution was observed for chromosomal regions encompassing the bx1 locus in determining lesion size but not the number of lesions or disease severity. Overall the contribution of loci in the vicinity of Ht1, Ht2 and bx1 was small relative to variation attributable to loci with quantitative effects identified in this study. Molecular-marker-facilitated mapping concurred with previous reciprocal translocation mapping studies on the importance of chromosomes 3, 5 and 7, despite the fact that these studies utilized diverse sources of resistant germplasm.
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  • 52
    ISSN: 1432-2242
    Keywords: Breeding ; Helminthosporium turcicum ; RFLP ; QTLs ; Disease-resistance ; Genetics
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    Notes: Abstract RFLPs were used to investigate components of host-plant response to Exserohilum turcicum in 150 unselected F2∶3 lines of a B52/Mo17 maize population. Following inoculation with spore suspensions of the pathogen (race 0), components of disease development were measured and then quantitative trait mapping was performed to identify the location and effects of quantitative trait loci (QTLs) determining host-plant response. Components of interest were the average number of lesions per leaf, the average percent leaf tissue diseased (severity) and the average size of lesions (cm2). Based on a LOD threshold of 2.31 (P〈0.05), the number of lesions appears to be associated with QTLs on chromosomes 1S, 3L, 5S. Severity was associated with analogous regions and, in addition, QTLs on chromosomes 7L and 8L. Most QTLs, for either of these two components, involve additive gene action and partial dominance or overdominance. In contrast, lesion size was associated with QTLs on chromosomes 7L and 5L; recessive gene action may be involved at 7L.
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  • 53
    ISSN: 1432-2242
    Keywords: Genetics ; Disease resistance ; Monocots
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    Notes: Abstract An F2 oat population was produced by crossing the diploid (n=7) species Avena strigosa (CI 3815) with A. wiestii (CI 1994), resistant and susceptible, respectively, to 40 isolates of Puccinia coronata, the causal agent of crown rust. Eighty-eight F2 individuals were used to construct an RFLP linkage map representing the A genome of cultivated hexaploid oat. Two hundred and eight RFLP loci have been placed into 10 linkage groups. This map covers 2416 cM, with an average of 12 cM between RFLP loci. Eighty-eight F3 lines, derived from F2 individuals used to construct the map, were screened for resistance to 9 isolates of P. coronata. One locus, Pca, was found to confer a dominant resistance phenotype to isolates 203, 258, 263, 264B, 290, 298, 325A, and 345. Pca also conferred resistance to isolate 276; however, an unlinked second gene may also be involved.
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    Behavioral ecology and sociobiology 34 (1994), S. 117-409 
    ISSN: 1432-0762
    Keywords: Social insects ; Apis mellifera ; Division of labor ; Genetics
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    Topics: Biology
    Notes: Abstract Variability exists among worker honey bees for components of division of labor. These components are of two types, those that affect foraging behavior and those that affect life-history characteristics of workers. Variable foraging behavior components are: the probability that foraging workers collect (1) pollen only; (2) nectar only; and (3) pollen and nectar on the same trip. Life history components are: (1) the age the workers initiate foraging behavior; (2) the length of the foraging life of a worker; and (3) worker length of life. We show how these components may interact to change the social organization of honey bee colonies and the lifetime foraging productivity of individual workers. Selection acting on foraging behavior components may result in changes in the proportion of workers collecting pollen and nectar. Selection acting on life-history components may affect the size of the foraging population and the distribution of workers between within nest and foraging activities. We suggest that these components define possible sociogenic “pathways” through which colony-level natural selection can change social organization. These pathways may be analogous to developmental pathways in the morphogenesis of individual organisms because small changes in behavioral or life history components of individual workers may lead to major changes in the organizational structure of colonies.
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    Behavioral ecology and sociobiology 35 (1994), S. 99-107 
    ISSN: 1432-0762
    Keywords: Key words Apis mellifera ; Genetics ; Drone production ; Allozymes ; Reproductive conflict
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previously we reported that there are subfamily differences in drone production in queenless honey bee colonies, but these biases are not always explained by subfamily differences in oviposition behavior. Here we determine whether these puzzling results are best explained by either inadequate sampling of the laying worker population or reproductive conflict among workers resulting in differential treatment of eggs and larvae. Using colonies composed of workers from electrophoretically distinct subfamilies, we collected samples of adult bees engaged in the following behavior: “true” egg laying, “false” egg laying, indeterminate egg laying, egg cannibalism, or nursing (contact with larvae). We also collected samples of drone brood at four different ages: 0 to 2.5-h-old eggs, 0 to 24-h-old eggs, 3 to 8-day-old larvae, and 9 to 14-day-old larvae and pupae. Allozyme analyses revealed significant subfamily differences in the likelihood of exhibiting egg laying, egg cannibalism, and nursing behavior, as well as significant subfamily differences in drone production. There were no subfamily differences among the different types of laying workers collected from each colony, suggesting that discrepancies between subfamily biases in egg-laying behavior and drone production are not due to inadequate sampling of the laying worker population. Subfamily biases in drone brood production within a colony changed significantly with brood age. Laying workers had significantly more developed ovaries than either egg cannibals or nurses, establishing a physiological correlate for the observed behavioral genetic differences. These results suggest there is reproductive conflict among subfamilies and individuals within queenless colonies of honey bees. The implications of these results for the evolution of reproductive conflict, in both queenright and queenless contexts, are discussed.
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    Behavioral ecology and sociobiology 34 (1994), S. 125-137 
    ISSN: 1432-0762
    Keywords: Social insects ; Apis mellifera ; Division of labor ; Genetics ; Nepotism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three experiments were performed to determine whether brood care in honey bee colonies is influenced by colony genetic structure and by social context. In experiment 1, there were significant genotypic biases in the relative likelihood of rearing queens or workers, based on observations of individually labeled workers of known age belonging to two visually distinguishable subfamilies. In experiment 2, no genotypic biases in the relative likelihood of rearing drones or workers was detected, in the same colonies that were used in experiment 1. In experiment 3, there again were significant genotypic differences in the likelihood of rearing queens or workers, based on electrophoretic analyses of workers from a set of colonies with allozyme subfamily markers. There also was an overall significant trend for colonies to show greater subfamily differences in queen rearing when the queens were sisters (half- and super-sisters) rather than unrelated, but these differences were not consistent from trial to trial for some colonies. Results of experiments 1 and 3 demonstrate genotypic differences in queen rearing, which has been reported previously based on more limited behavioral observations. Results from all three experiments suggest that genotypic differences in brood care are influenced by social context and may be more pronounced when workers have a theoretical opportunity to practice nepotism. Finally, we failed to detect persistent interindividual differences in bees from either subfamily in the tendency to rear queen brood, using two different statistical tests. This indicates that the probability of queen rearing was influenced by genotypic differences but not by the effect of prior queen-rearing experience. These results suggest that subfamilies within a colony can specialize on a particular task, such as queen rearing, without individual workers performing that task for extended periods of time.
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    Behavioral ecology and sociobiology 35 (1994), S. 99-107 
    ISSN: 1432-0762
    Keywords: Apis mellifera ; Genetics ; Drone production ; Allozymes ; Reproductive conflict
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    Topics: Biology
    Notes: Abstract Previously we reported that there are subfamily differences in drone production in queenless honey bee colonies, but these biases are not always explained by subfamily differences in oviposition behavior. Here we determine whether these puzzling results are best explained by either inadequate sampling of the laying worker population or reproductive conflict among workers resulting in differential treatment of eggs and larvae. Using colonies composed of workers from electrophoretically distinct subfamilies, we collected samples of adult bees engaged in the following behavior: “true” egg laying, “false” egg laying, indeterminate egg laying, egg cannibalism, or nursing (contact with larvae). We also collected samples of drone brood at four different ages: 0 to 2.5-h-old eggs, 0 to 24-h-old eggs, 3 to 8-day-old larvae, and 9 to 14-day-old larvae and pupae. Allozyme analyses revealed significant subfamily differences in the likelihood of exhibiting egg laying, egg cannibalism, and nursing behavior, as well as significant subfamily differences in drone production. There were no subfamily differences among the different types of laying workers collected from each colony, suggesting that discrepancies between subfamily biases in egg-laying behavior and drone production are not due to inadequate sampling of the laying worker population. Subfamily biases in drone brood production within a colony changed significantly with brood age. Laying workers had significantly more developed ovaries than either egg cannibals or nurses, establishing a physiological correlate for the observed behavioral genetic differences. These results suggest there is reproductive conflict among subfamilies and individuals within queenless colonies of honey bees. The implications of these results for the evolution of reproductive conflict, in both queenright and queenless contexts, are discussed.
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    Protoplasma 176 (1993), S. 53-63 
    ISSN: 1615-6102
    Keywords: Acetabularia acetabulum ; Gamete release ; Mating efficiency ; Mating physiology ; Gamete half-life ; Genetics
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    Topics: Biology
    Notes: Summary We have improved zygote recovery 11–1,000 fold by optimizing the physiology of gamete release and mating inAcetabularia acetabulum. Gamete release was affected by agar purity, concentration, and volume/gametangial pair. Cold pre-treatment of gametangia (14–30 d at 10°C in the dark) synchronized subsequent gamete release at 21°C in the light. Cold pre-treatment was nearly twice as effective in synchronizing subsequent gamete release when intact, gametangia-bearing caps rather than isolated gametangia were pretreated. Synchronizing gamete release doubled mating efficiency. In a wild-type laboratory strain ofA. acetabulum, there were 1,561±207 gametes/gametangium which had half-lives of 14.5 d in 0.1% seawater-agar. We recovered 48–93% of the expected numbers of zygotes from a mass mating of 8 to 1,226 gametangia and 11–128% of the expected numbers of zygotes from mating single gametangial pairs: the large range in the calculated mating efficiency may be attributable to the variation in the numbers of gametes made per gametangium. Zygote recovery from single gametangial pairs was highly dependent on the volume of mating matrix. In addition, most zygotes recovered were unattached to any other zygotes in the subsequent generation (〉 95% single cells from matings of 1–500 gametangial pairs). Our improvements in mating conditions and zygote recovery (1) have facilitated cell manipulation and culture ofA. acetabulum in the laboratory; and (2) have made controlled crosses for selection and genetic analysis of mutants feasible. These advances have removed a major barrier to genetic analysis of development inAcetabularia.
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    Plant ecology 50 (1982), S. 23-42 
    ISSN: 1573-5052
    Keywords: Beech ; Beechwoods ; Bioclimatology ; Fagus ; Genetics ; Mediterranean region ; Vegetation structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The study area is at the southern limit of the beech area, between the rivers Rhône in France and Ebro in Spain. From an analysis of the occurrence of beechwoods in this region and the pluviometric climate, it appears that part of the beechwood is found in a dry climate, which is still mediterranean. This argument seems sufficient to justify the existence of a ‘mediterranean’ beechwood, at least on a climatic level. Beechwood when occurring in a dry climate has special characteristics: - The development of the wood differs with the climate. Young beeches need shadow in a dry climate; they can grow in full light in a moist climate. - The floristic composition is different. - The understorey of dry woods is less structured than that of moist woods. - The genetic structure as measured in the enzymatic system Px1 is polymorphic in the dry and monomorphic in the moist conditions studied. It would be interesting to verify if the characters noted in a dry mediterranean climate are also present in dry atlantic or continental beechwoods. Since the mediterranean climate is drier and more unfavorable to beech than other climates, the particular characters of ‘dry’ beechwoods will be more pronounced near the Mediterranean.
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    Biology and philosophy 5 (1990), S. 349-371 
    ISSN: 1572-8404
    Keywords: Genetics ; gene structure ; hereditary unit
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    Topics: Biology , Philosophy
    Notes: Abstract Definitions of the term ‘gene’ typically superimpose molecular genetics onto Mendelism. What emerges are persistent attempts to regard the gene as a ‘unit’ of structure and/or function, language that creates multiple meanings for the term and fails to acknowledge the diversity of gene architecture. I argue that coherence at the molecular level requires abandonment of the classical unit concept and recognition that a gene is constructed from an assemblage of domains. Hence, a domain set (1) conforms more closely to empirical evidence for genetic organization of DNA regions capable of transcription and (2) has ontological properties lacking in the traditional unit definition.
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  • 61
    ISSN: 1573-739X
    Keywords: Antimalarials ; Artemisia annua L ; Artemisinin ; Biosynthesis ; Chemistry ; Clinical trials ; Pharmacology ; Phytochemistry ; Sesquiterpenes ; Toxicology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Artemisinin, a sesquiterpene lactone endoperoxide isolated fromArtemisia annua L., and a number of its semisynthetic derivatives have shown to possess antimalarial properties. They are all eflective againstPlasmodium parasites that are resistant to the newest and commonly used antimalarial drugs. This article gives a survey of the literature dealing with artemisinin-relaled antimalarial issues that have appeared from the end of 1989 up to the beginning of 1994. A broad range of medical and pharmaceutical disciplines is covered, including phytochemical aspects like the selection of high-producing plants, analytical procedures, and plant biotechnology. Furthermore, the organic synthesis of artemisinin derivatives is discussed, as well as their mechanism of action and antimalarial activity, metabolism and pharmacokinetics, clinical studies, sideeffects and toxicology, and biological activities other than antimalarial activity.
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    Pharmacy world & science 13 (1991), S. 109-118 
    ISSN: 1573-739X
    Keywords: Chemistry ; Consumer product safety ; Drug packaging ; Environmental pollution ; Plasticizers ; Polyvinyls
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract In this report the state of the art with respect to PVC as pharmaceutical packaging material is described. A general introduction into the applications of PVC is followed by a description of its production process. The metabolic effects of the monomer of PVC, vinyl chloride and of the most commonly used plasticizer diethylhexylphthalate are mentioned. Special attention is given to the pharmaceutical properties of plasticized PVC bags in comparison to other plastics and the environmental aspects of waste PVC disposal. Although there are emotional and political queries regarding the future use of PVC as a (pharmaceutical) packaging material, we conclude that there is no scientific justification for a total or partial ban of PVC. PVC will remain a fact of life as a cheap, versatile, high-performance and well-investigated plastic material for medical and pharmaceutical applications, to be replaced by newer plastics only for certain well-defined indications where the requirements of the plastic to be used are so specific that it will economically and technically be justified to use another polymer. Community and hospital pharmacists have to be prepared for a role in intake of waste plastic disposables, probably against deposit money, in order to fulfil the logistics needed for recycling.
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    Plant systematics and evolution 171 (1990), S. 1-14 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Compositae ; Chemistry ; systematics ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The broad knowledge of the chemistry of theCompositae allows the discussion of its relevance for the systematics and evolution within the family. Furthermore a separation into subfamilies can be supported by the observed differences in the distribution of the main constituents in the tribes.
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  • 64
    ISSN: 1617-4623
    Keywords: Gene regulation ; Ribozyme ; npt-gene ; Transgenic tobacco ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A chimeric gene encoding a ribozyme under the control of the cauliflower mosaic virus (CaMV) 35S promoter was introduced into transgenic tobacco plants. In vivo activity of this ribozyme, which was designed to cleave npt mRNA, was previously demonstrated by transient expression assays in plant protoplasts. The ribozyme gene was transferred into transgenic tobacco plants expressing an rbcS-npt chimeric gene as an indicator. Five double transformants out of sixteen exhibited a reduction in the amount of active NPT enzyme. To measure the amount of ribozyme produced, in the absence of its target, the ribozyme and target genes were separated by genetic segregation. The steady-state concentrations of ribozyme and target RNA were shown to be similar in the resulting single transformants. Direct evidence for a correlation between reduced npt gene expression and ribozyme expression was provided by crossing a plant containing only the ribozyme gene with a transgenic plant expressing the npt gene under control of the 35S promoter, i.e. the same promoter used to direct ribozyme expression. The expression of npt was reduced in all progeny containing both transgenes. Both steady-state levels of npt mRNA and amounts of active NPT enzyme are decreased. In addition, our data indicate that, at least in stable transformants, a large excess of ribozyme over target is not a prerequisite for achieving a significant reduction in target gene expression.
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    Molecular genetics and genomics 228 (1991), S. 361-371 
    ISSN: 1617-4623
    Keywords: Plant ; Hormone ; Genetics ; Hypocotyl ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated nine independent auxin-resistant mutants of Nicotiana plumbaginifolia by culturing M2 seedlings in the presence of indole-3-acetic acid ethyl ester or 1-naphthaleneacetic acid at concentrations which significantly inhibit hypocotyl elongation of the wild type. The mutations were induced by treating seed with ethyl methanesulphonate and were found in the course of screening 10 000 individual M2 families. Auxin resistance was in all cases the result of a mutation at a single, nuclear locus. The dominance relationships of two of the mutants could be defined as recessive or dominant; all other mutants showed partial dominance. In contrast to previously described mutants of Arabidopsis and N. plumbaginifolia, all of the present mutants were specifically resistant to auxin; the mutants were cross-resistant to several auxins, but showed no increased resistance to cytokinin, abscisic acid, ethylene or 1-amino-cyclopropane-1-carboxylic acid. The importance of the choice of the selection criterion for the isolation of specific resistance traits is discussed.
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  • 66
    ISSN: 1573-5133
    Keywords: Demography ; Genetics ; Geographic variation ; Stochasticity ; Fluctuating environments ; Allele frequencies ; River ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Synopsis The purpose of this study was to determine the effects of unpredictable environmental fluctuations on the demographic and genetic structure of Fundulus zebrinus populations. Collections of F. zebrinus were taken from three rivers in the Arkansas River basin: the Arkansas, Chikaskia, and Ninnescah. Fish were sampled from three sites on each river on nine collection dates throughout 1984 and 1985. Totals of 2100 fish and 6000 fish were included in electrophoretic and demographic analyses, respectively. The results of the study indicate that within a limited geographic region (i.e. within rivers) spatial differences and temporal changes in both demographic and genetic population characteristics occur frequently and are primarily stochastic. However, on a larger spatial scale (i.e. across rivers), general trends emerge for demographic and especially for genetic population characteristics. These results illustrate the importance of sampling scale for conclusions of life-history evolution in fluctuating environments. In addition, it was found that regulation of Fundulus zebrinus populations includes an important density-independent component. Stochastic demographic differences across space and changes through time and spatially and temporally heterogeneous allele frequencies, are both indicative of density-independent regulation. Variation in population parameters, both demographic and genetic, was observed between populations sampled from each river. These population differences were attributed to differences between the rivers themselves.
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    Environmental biology of fishes 6 (1981), S. 357-360 
    ISSN: 1573-5133
    Keywords: Behaviour ; Esterase ; Evolution ; Genetics ; Isozymes ; Stock structure ; Schools ; Starch gel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Synopsis Heterogeneous gene frequencies of Est-1 across groups ofNotropis cornutus provide evidence of behaviourally imposed restrictions on stock structuring. Positive fixation indices (F1S = 0.056 and F1T = 0.085) were reflected by a deficiency of heterozygotes for pooled groups. The degree of subdivision ofN. cornutus stocks cannot be evaluated with the present evidence. but it is likely that their schooling behaviour is associated with significant genotypic structuring of the species.
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    Environmental biology of fishes 8 (1983), S. 235-247 
    ISSN: 1573-5133
    Keywords: Amelanism ; Aggression ; Assessing mates ; Assortative mating ; Cheaters ; Chemical communication ; Coloration ; Context model ; Dominance ; Dummies ; Genetics ; Inhibition model ; Metamorphosis ; Nicaragua ; Parental care ; Polymorphism ; Sexual imprinting ; Visual communication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Synopsis The midas cichlid,Cichlasoma citrinellum, occurs in the lakes of Nicaragua. In semi-turbid to turbid lakes about 8% of the adults are amelanic, having lost their melanophores at various ages, and are thus yellow through red and sometimes white. The commonest hues are yellow through orange, called gold. Gold morphs ought to be selected against because they are probably conspicuous to predators and they cannot communicate by changing markings. To maintain the polymorphism, gold coloration must have offsetting advantages. Gold morphs dominate normal ones of equal size, and that improves their access to limiting resources. Gold morphs, however, do not seem to be intrinsically more aggressive but rather attain dominance through the effect of their color on their opponents. This gold effect is affected by experience; it is enhanced by sharing the color of the dominant fish in a group, and by being rare. The midas cichlid mates assortatively but imperfectly. Choice of mate is influenced by color of self and of parents and can be constrained by size-color relationship.
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  • 69
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    Journal of chemical ecology 16 (1990), S. 2935-2946 
    ISSN: 1573-1561
    Keywords: Genetics ; sex pheromone ; Lepidoptera ; Noctuidae ; Trichoplusia ni ; cabbage looper moth ; reproductive isolation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The genetic basis of interpopulational differences in the pheromone blend emitted by the cabbage looper moth,Trichoplusia ni (Hübner), was examined by crossing individuals from a field-derived population (P1) with individuals from a long-maintained laboratory colony (P2). These colonies differed in the emission rate and relative proportions of four of the five known minor pheromone components, but not in the emission rate of the major component, (Z)-7-dodecenyl acetate (Z7-12∶Ac). These differences in pheromone blend were quantitatively small but biologically significant, because in the field, males responded preferentially to traps baited with a pheromone blend that is similar to that emitted by P1 females relative to a blend similar to that emitted by P2 females. In initial crosses, variation in the quantity and quality of pheromone blends among families of P1, P2, and F1 hybrid females was examined. In F1 females the relative proportions (quantity relative to the major component) of (Z)-5-dodecenyl acetate (Z5-12∶Ac) and (Z)-7-tetradecenyl acetate (Z7-14∶Ac) were intermediate to parental lines. In a second more extensive set of crosses, analyses included P1, P2, F1, F2, and selected backcrosses. The relative proportion of Z5-12∶Ac, Z7-14∶Ac, and Z9-14∶Ac emitted by F1 females were intermediate to parental lines. The frequency distributions of relative proportions of these components emitted by females were not consistent with those expected under a single autosomal or sex-linked gene hypothesis, suggesting that more than one gene is involved in the quantitative differences in the pheromone blend.
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  • 70
    ISSN: 1573-5060
    Keywords: Brassica oleracea ; Cauliflower ; Stalk rot ; Screening ; Genetics ; Resistance ; Sclerotinia sclerotiorum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The inheritance of resistance in cauliflower to stalk rot (Sclerotinia sclerotiorum (Lib.) de Bary) was investigated in population from six generations of six crosses. Disease incidence was recorded on 4 parents, 6 Fs 1, 6 Fs 2 and 12 back-crosses in a screenhouse under artificially created epiphytotic conditions. Resistance to stalk rot in this set of parents was found to be polygenic and under the control of recessive genes and due primarily to additive gene action. A breeding strategy emphasizing recurrent selection should lead to improvement in resistance.
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    Angewandte Makromolekulare Chemie 175 (1990), S. 107-116 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Zur Beschreibung der Polymerisationsgeschwindigkeit der radikalischen Fallungspolymerisation von Acrylsäure in Toluol wurde ein Modell entewickelt. Die experimentelle Überprüfung des Modells erfolgte durch Messungen in einem isotherm betriebenen Reaktionskalorimeter. Das Modell beschreibt die Polymerisationsgeschwindigkeit bis zum vollständigen Umsatz in einem Temperaturbereich von 40 bis 60°C.
    Notes: A kinetic model for the free-radical precipitation polymerization of acrylic acid in toluene was developed and verified experimentally by using an isothermal reaction calorimeter. The model predicts the rate of polymerization accurately up to complete conversion in the temperature range of 40 to 60°C.
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  • 72
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    Angewandte Makromolekulare Chemie 175 (1990), S. 157-168 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Es wird über Untersuchungen an Legierungen aus isotaktischem Polypropylen und EPDM-Elastomer berichtet, die mit hochenergetischen Elektronen bestrahlt worden waren. Die Mischungsverhältnisse in den Proben überstrichen sowohl den für elastomermodifizierte Thermoplaste typischen Bereich als auch den der thermoplasticschen Elastomeren. Eine Vernetzung der Komponenten über die Grenzfläche hinweg wurde nachgewiesen. Diese grenzflächenübergreifende Vernetzung bewirkte eine merkliche Verbesserung der mechanischen Eigenschaften insbesondere des thermoplastischen Elastomeren.
    Notes: Blends of isotactic polypropylene and EPDM rubber have been treated by electron irradiation in the composition range of rubber modification and in the thermoplastic rubber composition range as well. Mutual crosslinking of the components through the interface could be detected. This intercrosslinking caused a remarkable improvement of the mechanical properties particularly of the thermoplastic rubber.
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    Angewandte Makromolekulare Chemie 175 (1990), S. 205-205 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
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  • 74
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The mechanisms of photooxidation usually envisaged, are perfected by taking into account photoreactions of peroxy radicals. They involve interaction of a peroxy radical with the polymer to yield a hydroperoxy radical and a pair of macroradicals. After peroxidation, this pair will usually undergo bimolecular termination. The influence of such a reaction on photooxidation kinetics is particularly important for polyethylene. Then, chain termination occurs mainly by interaction of two hydroperoxy radicals or of a hydroperoxy radical with a macroperoxy radical. The reaction does not only affect kinetics of photooxidation but also the nature of the photooxidation products. The latter is more important for polypropylene than for polyethylene.
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  • 75
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Isothermal Differential Thermal Analysis was used to test the thermooxidative stability of pipes of polybutene during internal pressure tests at 110°C. At low hoop stresses and long testing times pipe failure was exclusively caused by thermooxidative degradation of the polymer.The stabilizer distribution in the initial state and its alteration during the pressure tests could easily be measured by isothermal DTA. Leaching of antioxidants from pipe walls could be detected. The decrease of residual thermooxidative stability was degressive.Degradation of pipe material started from the exterior pipe wall. Molecular weight of polybutene decreased rapidly after stabilizer exhaustion which could be observed by isothermal DTA.
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  • 76
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: A base of experimental data on the ageing of commerial grade plastics is created. In includes assorted physical and chemical properties of plastics and their changes at natural ageing. An automated system for storing and processing of the experimental data is designed. Processing involves mathematical modeling of the changes in properties that aids in empirical prediction. The possibilities of the system are verified with experimental results from a 5-year ageing period of PE-LD and PE-HD. The results show a good agreement between the predicted and real data for selected models.
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    Angewandte Makromolekulare Chemie 179 (1990), S. 35-56 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Die Morphologie von Polypropylen-Spritzgußteilen mit Bindenaht wurde mit Röntgenweit-und Kleinwinkelstreuung, Lichtstreuung und Polarisationsmikroskopie untersucht. Die erhaltenen morphologischen Daten wurden mit mechanischen Werten aus Zugversuchen korreliert. Es wird gefunden, daß die mechanischen Eigenschaften der untersuchten Proben im wesentlichen von der sphärolithstruktur beeinflußt werden. Anhäufungen von Sphärolithen mit Kristallen der hexagonalen β-Modifikation und eine starke Inhomogenität der Sphärolithstruktur senkrecht zur Bindenaht sind der wesentliche Grund für die verschlechterten mechanischen Eigenschaften. Diese Sphärolithstruktur wird durch die Temperatur des Spritzgußwerkzeugs stark beeinflußt. Die Lamellen-Überstruktur hat dagegen einen vernachlässigbar kleinen Einfluß auf das mechanische Verhalten.
    Notes: The morphology of polypropylene plaques containing a weld line, has been investigated by x-ray wide and small angle scattering, light scattering and polarization microscopy. The results are correlated to mechanical values obtained from tensile tests. While the lamellar morphology is of neglectable influence, it is found, that the mechanical properties of the samples are strongly influenced by the spherulitic structure. Clustering of β-type spherulites and strong morphological inhomogeneities in the vicinity of the weld line are the primary reason for the mechanical weakening of the plaques. The temperature of the mould has a strong effect on the spherulitic structure and thus on the tensile properties of the samples.
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    Angewandte Makromolekulare Chemie 179 (1990), S. 77-85 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Füllstoffe für PVC- und PP-Formmassen wurden mit Mikrowellenplasma behandelt. Photoakustische FT-IR-Spektren zeigen, daß die Plasma-Polymerhülle auf der Füllstoffoberfläche —C=C—, —CH2—, CH3— und andere Gruppen enthält. Aus Röntgenelektronenspektren geht hervor, daß zwischen Siliciumdioxid und der organischen Beschichtung chemische Bindungen existieren. Durch Compoundieren von plasmabehandelten Füllstoffen mit PVC und PP werden Materialien mit guten mechanischen Eigenschaften bei niedrigen Kosten erhalten.
    Notes: Fillers for PVC and PP compounds have been treated by microwave plasma. FT-IR photoacoustic spectroscopy studies show that the plasma polymer sheath on filler's surface contains —C=C—, —CH2—, —CH3 groups, etc. X-ray photoelectron spectroscopy studies reveal that interfacial chemical bondings exist between silica and the organic coating. Application of plasma-treated fillers in PVC and PP compounds gives rise to materials of good mechanical properties and low cost.
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    Angewandte Makromolekulare Chemie 179 (1990), S. 135-148 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Poly-(alkylenadipat)-diole mit Molmassen von 1500, 2000 und 3000 wurden bei Temperaturen von 25 und 60°C in Gegenwart verschiedener Alkohole und unterschiedlicher PUR-Katalysatoren (1,4-Diazabicyclo[2.2.2]octan (DABCO), Dibutylzinndilaurat (DBTL),Dibutylzinndiethoxid (DBTEO) und Dibutylzinnoxid (DBTO)) in Ethylacetat bzw. Toluol gelagert und die Alkoholyse verfolgt. Die erhaltenen Ergebnisse werden mit dem Alkoholyseverhalten verschiedener Dialkyladipate sowie des Lösungsmittels Ethylacetat unter den selben Bedingungen verglichen. Die Alkoholyse verlauft in Gegenwart der zinnorganischen Verbindungen etwa mit gleicher Geschwindigkeit, ohne daß diese selbst signifikant zersetzt werden, wahrend DABCO praktisch inaktiv ist. Die Aktivitat der Alkohole ist starker von ihrer sterischen Struktur als von ihrer Basizitat abhangig. Die Stabilität der Polyalkylenadipate gegenüber einer Umesterung nimmt mit der Lange der Diolalkyleneinheiten des Polyols zu. Die Umesterung von Adipinsäuredialkylestern findet auch partiell in Anwesenheit eines großen Uberschusses an Ethylacetat statt.
    Notes: Poly(alkylene adipate)diols having molar masses of 1500, 2000 and 3000 were stored at temperatures of 25 and 60°C in the presence of low amounts of different alcohols and some polyurethane (PUR) catalysts, such as dibutyltindilaurate (DBTL), dibutyltindiethoxide (DBTEO), dibutyltinoxide (DBTO), and 1,4-diazabicyclo[2.2.2]octane (DABCO). Alcoholysis was studied using either ethyl acetate (EA) or toluene as solvent. The results were compared with those obtained by examining the behaviour of several dialkyl adipates and ethyl acetate under identical conditions. The alcoholysis in the presence of the tin catalysts proceeds at the same reaction rate without significant decomposition of the catalyst, whereas DABCO is practically inactive. The nucleophilic activity of the alcohols depends on their bulk size rather than their basicity. The resistance of polyalkylene adipates to transesterification increases with the increasing alkyl chain length of diol units in polyester. A partial transesterification of dialkyl adipates takes place too, in spite of ethyl acetate being present in excess.
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    Angewandte Makromolekulare Chemie 179 (1990), S. 157-171 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Es wurde das Permeations- und Trennverhalten von Alkohol/Wasser-Systemen durch poröse PVA-Membranen untersucht. PVA-Membranen mit unterschiedlicher Anzahl und Große von Poren wurden über Mischungen in Lösung mit verschiedenen synthetisierten Polymeren und Copolymeren, wie Polyacrylsäure, Polyacrylnitril, Polyacrylamid, Poly(Methylmethacrylat-co-Maleinsäureanhydrid) etc., anschließendes Gießen von Filmen und Extraktion der Polymeren oder Copolymeren durch Lösungsmittel hergestellt. Die Abhängigkeit der Durchlässigkeit und Trennwirkung von der Größe und Form der permeierenden Moleköle wurde qualitativ diskutiert. Des weiteren wurde die Selektivität der Trennwirkung in Abhangigkeit von der Zusammensetzung von Alkohol/Wasser-Gemischen und von der Größe und Anzahl der Poren untersucht. Es wurde gefunden, daß die Selektivität von der Menge und dem Molekulargewicht der in die Membranen eingebrachten Polymeren abhängt. Wenn der Gewichtsanteil des verwendeten Polymeren größer als 0,1 war, permeierte bevorzugt der Alkoholanteil bei methanolischen Msungen, wobei der Trennfaktor mit steigender Methanolkonzentration anstieg. In Gemischen mit anderen Alkoholen zeigten die Membranen andererseits eine selektive Durchlässigkeit für Wasser. Der Einfluß der Versuchsbedingungen wurde ebenfalls untersucht.
    Notes: The permeation and separation characteristics of alcohol/water systems through porous PVA membranes were investigated. Porous PVA membranes with different pore size and number were prepared by solution blending of PVA with several synthesized polymers or copolymers, such as polyacrylic acid, polyacrylamide, polyacrylonitrile, and methylmethacrylate-co-maleic anhydride, etc. Then casting, and finally extracting the blended polymers or copolymers by solvent from the membranes. The dependency of both permeation and separation on the molecular size and shape of the permeating species was dicussed qualitatively. Moreover, the permselectivity was investigated with attention to the feed composition of alcohol/water mixture and the effect of pore size and number. The selectivity was found to depend on the weight ratio and the molecular weight of polymer introduced to the membrane. When the weight ratio of polymer introduced into the membrane was larger than 0.1, methanol was permeated through membrane preferentially in methanol/water system, and the separation factor increased with increasing the methanol feed concentration. On the other hand, membrane had a selective permeability for water in the other alcohol/water systems. The influence of operating conditions was also studied.
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    Angewandte Makromolekulare Chemie 175 (1990), S. 29-38 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Die Temperatur des T1-Minimums für die Segmentbewegung in Polypropylen, das einen hohen ataktischen Anteil besitzt, ist niedriger als die in isotaktischem Polypropylen. Ein kurzes T1, T1s, tritt bei beiden Materialien auf. Bei Temperaturerhöhung nimmt T2a (T2 der beweglichen amorphen Bezirke) stark und T2m (T2 der dazwischen liegenden Bezirke) leicht zu, während sich T2c (T2 der steifen kristallinen Bezirke) kaum ändert. T2a in Polypropylen mit hohem ataktischem Anteil ist länger als in isotaktischem Polypropylen.Der Massenbruch der kristallinen Bezirke, Fc, nimmt bei Temperaturerhöhung ab und der der amorphen Bezirke, Fa, zu. Die Relaxationsgeschwindigkeit, 1/ (T1min), steigt linear mit Zunahme des amorphen Anteils an, außer bei der mit siedendem n-Heptan extrahierten Probe. Mit Zunahme der Peaktemperatur von tan δ fällt T2a nahezu linear ab, während die Temperatur des T1-Minimums zunimmt.
    Notes: The temperature of the T1 minimum for the segmental motion in polypropylene containing a lot of atactic fraction is lower than that in isotactic polypropylene. A short T1, T1s, appears for both materials. As the temperature is raised, there is a large increase in T2a (T2 of the mobile amorphous regions), and a mild increase in T2m (T2 of the intermediate regions), while there is no substantial change in T2c (T2 of the rigid crystalline regions). T2a in polypropylene containing a lot of atactic fraction is longer than that in isotactic polypropylene.The mass fraction of crystalline regions, Fc, decreases and that of the amorphous regions, Fa, increases as the temperature is raised. There is a linear increase in the relaxation rate, 1/(T1min), as the amorphous fraction is increased, except for the sample extracted with boiling n-heptane. With increase of the peak temperature of tanδ, T2a decreases nearly linearly, while the temperature of the T1 minimum increases.
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    Angewandte Makromolekulare Chemie 180 (1990), S. 191-198 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Die Bestrahlung von PTFE mit Elektronen niedriger Energie führt zur Abspaltung von Fluor und zum Einbau von Sauerstoff in die Polymeroberfläche. Es wird gezeigt, daß das Ausmaß der Oberflächenveränderungen hauptsächlich von der Zahl und kaum von der Energie der Elektronen abhängt, die die Oberfläche erreichen. Änderungen in der Oberflächenenergie resultieren in unterschiedlichem Benetzungsverhalten. Kontaktwinkelmessungen und Kurvenanalysen der C-1s-Peaks aus Röntgenphotonenspektroskopiemessungen zeigen, daß der am wenigsten benetzbare Teil der bestrahlten Oberflächen in den meisten Fällen aus —CF2 —CF-Einheiten besteht. Werden bei der Bestrahlung nahezu alle Fluoratome abgespalten, bleiben nur isolierte —CF2-oder —CF-Gruppen zurück. Die „receding“-Winkel werden mit zunehmender Sauerstoffkonzentration in der Oberfläche kleiner, was auf die Bildung stark oxidierter Bereiche hinweist. Die Unabhängigkeit der Kontaktwinkel von der Alterungsdauer deutet auf eine sehr geringe Beweglichkeit der Makromoleküle hin, was mit einer starken Vernet-zung an der Oberfläche zusammenhängen könnte.
    Notes: Low energy electron irradiation of PTFE induced fluorine depletion and introduction of oxygen on the surface. The effect on surface chemistry was shown to be dependent mostly on the amount of electrons reaching the sample, rather than on their energy.Changes in surface energy resulted in different wetting behaviors. The measurement of advancing water contact angles and the analysis of XPS C-1s peak shapes showed that the least wettable portion of irradiated surfaces is made in most cases of —CF2—CF — units. When the treatment caused a nearly complete fluorine depletion, only isolated —CF2— or —CF— groups are present. Receding angle values decreased linearly depending on the amount of oxygen introduced on the sample during the treatment, suggesting the formation of strongly oxidized polymer islets. The invariance of contact angles with aging time indicated very low macromolecular mobility, probably related to extensive surface crosslinking.
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    Angewandte Makromolekulare Chemie 181 (1990), S. 41-52 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: In aqueous dispersions of anionic polyurethanes containing the triethylammonium salt of 2,2-dimethylol propionic acid as chain extender, triethylamine can be largely replaced by ammonia. For electrostatic reasons, NH4+ is namely more tightly bound in the ion pairs located in the Stern layer than NH(C2H5)3+. Thermal treatment of films leads to a loss of base, whereby ammonia is more rapidly released than triethylamine. Therefore, crosslinking with partially methylated hexamethylolmelamine proceeds the faster the more triethylamine is replaced by ammonia.
    Notes: In Dispersionen aus anionischen Polyurethanen, die das Triethylammoniumsalz der 2,2-Dimethylolpropionsäure als Kettenverlängerungsmittel enthalten, läßt sich durch Zugabe von Ammoniak eine weitgehende Verdrängung des Triethylamins erreichen. Aus elektrostatischen Gründen ist nämlich in den in der Sternschen Schicht lokalisierten Ionenpaaren NH4+ fester gebunden als NH(C2H5)3+. Bei der thermischen Behandlung der Filme tritt Basenabspaltung ein, dabei erfolgt die Abgabe von Ammoniak deutlich schneller als die von Triethylamin. Die Spannungswerte der mit teilmethyliertem Hexamethylolmelamin vernetzten Proben sind daher um so höher, je mehr Triethylamin durch Ammoniak ersetzt wurde.
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    Angewandte Makromolekulare Chemie 181 (1990), S. 53-66 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Die Viskoelastizitäts-Parameter von geschmolzenem Polystyrol (PS) wurden mit einem Instron-Kapillarrheometer gemessen.Die Drucknachgiebigkeit B(t) zeigt ein Plateau sowohl im Schmelze (B1)- als auch im Glaszustand (Bg); beide nehmen mit abnehmender Spannung ab. Durch Verschiebung der B(t)-Kurven werden Master-Kurven erhalten, mit denen das Gesamtdruckverhalten (überlagerte elastische und viskose Deformationen) analysiert werden kann. Die stationäre Nachgiebigkeit (Bs) erlaubt eine Beschreibung des elastischen Energieanteils und scheint mit der Extrusions-Strangaufweitung (Bcs/Bds) korreliert zu sein. Die Volumenviskosität (ηk) nimmt mit zunehmender Spannung (P), zunehmender Belastungsgeschwindigkeit (k) und abnehmender Temperatur ab.
    Notes: Viscoelastic parameters of polystyrene (PS) melt in compression creep have been measured in an lnstron capillary-rheometer.Bulk compression creep compliance B(t) shows plateau regions in the molten state (B1) and the glassy state (Bg), both decreasing with increasing stress. Shifting of B(t) curves provides master curves suitable to analysing the total (superposed elastic and viscous deformations) bulk compression behaviour. The steady-state creep compliance (Bs) allows to describe the recoverable elastic energy (Be) and seems to be related to the extrusion die swell (Bcs/Bds). Volume viscosity (ηk) decreases with decreasing stress (P), increasing compression rates (k) and decreasing temperature (T).
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  • 85
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    Angewandte Makromolekulare Chemie 181 (1990), S. 67-74 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Es wurden Untersuchungen über Molekulargewichtsänderungen an physikalisch bei Raumtemperatur gealterten isotaktischen Polypropylenfilamenten (iPP) mit Hilfe der Hochtemperaturgelpermeationschromatographie (HTGPC) durchgeführt. Das Ergebnis der Untersuchungen weist auf eine sehr unbedeutende Änderung des Molekulargewichts des Materials während der Alterung hin.Das Materialgranulat wurde als Bezugsstandard gewählt. Basierend auf der Uneinheitlichkeit D = Mw/Mn des Granulats wurden Filamente der Handelsprodukte GYE 47 und PXC 31399 charakterisiert, deren Alterungsdauer zwischen 2 Wochen und 8 Monaten lag. Bei den Filamenten der Qpe GYE 47 verringerte sich die Breite der Molekulargewichtsverteilung D um durchschnittlich 12%. Andererseits zeigen die Filamente des PXC 31399-vps einen weniger eindeutigen Trend zur Änderung, jedoch im Mittel hat sich die Breite der Verteilung um etwa 20% erniedrigt. Bei letzterem ist die Verteilung iiber die Alterungsdauer stark gestreut. Der GYE 47-Typ zeigt eine nahezu asymptotische Abnahme. Es wird angenommen, daß die Ungleichartigkeit in der Ändenz der Anderung von D zwischen beiden Proben in ihrer unterschiedlichen thermodynamischen Stabilitat liegen ktinnte; PXC 31399 ist weniger stabil.
    Notes: Experiments on the molecular weight variations of physically, room temperature aged isotactic polypropylene (iPP) filaments were done using the High Temperature Gel Permeation Chromatography (HTGPC). Evidence emanating from the studies indicates a very marginal change in molecular weight of the materials during aging.The granules of the materials were chosen as the reference standard. Based on the polydispersity D = Mw/Mn of the granules, filaments of commercial grades GYE 47 and PXC 31399 were characterized between an aging period ranging from 2 weeks to 8 months. The filaments of the GYE 47 grade have their distributions D lowered on the average by about 12%. On the other hand, those of the PXC 31399 grade have a less definite trend in change, but on the average, D is lowered by about 20%. The distributions of this latter grade are highly scattered over the period of aging. The GYE 47 grade shows a reduction in the distribution of a near asymptotic nature. It is believed that the disparity in the trend of change of D between the two samples could be attributed to their relative thermodynamic stability with the PXC 31399 grade being less stable.
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  • 86
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The properties (squared dimensions, anisotropy, numbers of intra- and intermolecular contacts) of a single five-way cubic lattice chain embedded into an environment (matrix) of chains of the same length n = 50 were evaluated as a function of matrix volume fraction (matrix density) v and intermolecular interaction between the matrix polymer and the minority chain segments, characterized by a parameter φ. No convincing evidence was found for the occurrence of a coil-globule transition in the range of matrix densities (v ≤ 0.7) and repulsive interactions between matrix and minority chain (φ ≤ 0.2) investigated. For moderate attractive interaction (φ ≈ -0.17) a compensation of the chaincompressing action of the matrix and the chain-expanding interaction with the matrix was observed resulting in a zero-dependence of the size and shape of the minority chain on matrix density. It further turned out that there are fixed relations among the various size and shape data irrespective of the specific combination of matrix density and thermodynamic interaction by which a particular polymer dimension is produced. These interrelations are fairly the same as those evaluated for isolated chains the size of which is varied by an intramolecular energy parameter φi.
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  • 87
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: New three phases composite materials made of glassbeads as filler, water as liquid dispersed phase and polystyrene as matrix were obtained by polymerization of a water in oil emulsion formed by water dispersed in styrene in which glass beads are added. For this purpose glass beads have to be modified by silanization by a coupling agent 3-(Trimethoxysilyl)propylmethacrylate (TPM) or a silane Octadecyltrichlorosilane (ODMS), in order to prevent the wetting of the glass by water. Only low coverage of the glass surface by the coupling agent (0,05% of TPM) are convenient for the preparation cellular materials having good mechanical properties. At higher coverage ratio, strong hydrophobic glass beads are obtained which destabilize the water / styrene emulsion and lead to macroporous products. Study of the rheology of the filled emulsion appears to be the more simple and more sensitive method to determine the level of wettability of the glass bead by the emulsion which is the most significant parameter for the preparation of such three phases composite materials. Composite materials made of glass beads, air and polystyrene are simply obtained by evaporation of water under vacuum.
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  • 88
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: To test the molecular parameters concerning gas sorption in polymers, the concentration of CO2, N2O, CO, N2, CH4 and the noble gases Ne, He in glassy poly(butylene terephthalate) films (PBTP) has been studied gravimetrically with a recording microbalance at 25°C. The sorption isotherms exhibit downward curvature to the pressure axis. As neither solubility nor adsorption can explain the experimental results, analysis was carried out based on the dual-sorption model: gas dissolution and microvoid filling are considered as independent sorption mechanisms. The parameters of the dual-sorption model for the mentioned penetrants are determined. The results indicate that for parameter correlation the Lennard-Jones potential parameters give a rough idea, but size exclusion of gases in small diameter microvoids is proposed and special chemical interactions must be considered.
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  • 89
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    Angewandte Makromolekulare Chemie 185 (1991), S. 283-292 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: The ring opening metathesis polymerisation (ROMP) of 5-cyano-2-norbornene with tungsten hexachloride, tungesten oxytetrachloride, bis(dichloralkoxo)tungsten tetrachloride and bis(dichloralkoxo)tungsten oxydichloride as transition metal component was studied. Methylaluminoxan, diethylaluminium ethoxide, tetraisobutyldialuminoxan and tetrabutyltin served as cocatalyst. Toluene, Chlorobenzene and dichloromethane were used as solvents. The concentration of the tungsten components was in the range from 5 × 10-4 to 5 × 10-3 mol/l. Among the catalyst studied, tungsten hexachloride with diethylaluminium ethoxide proved to be the best. The polymerisation was highly selective with this system even at high monomer/tungsten ratios (e.q. 10200:1) and let to high conversions (≥82%).
    Notes: Die ringöffnende metathetische Polymerisation (ROMP) von 5-Cyano-2-norbornen wurde unter Einsatz von Wolframhexachlorid, Wolframoxidterachlorid, Bis(dichloralkoxo)wolframtetrachlorid und Bis(dichloralkoxo)wolframoxiddichlorid als Übergangsmetallkomponente untersucht. Als Cokatalysatoren dienten Methylaluminoxan, Diethylaluminiumethoxid, Tetraisobutyldialuminoxan und Tetrabutylzinn. Als Lösungsmittel wurden Toluol, Chlorbenzol und Dichlormethan verwendet. Die Konzentration der Wolframkomponente lag im Bereich 5 × 10-4 - 5 × 10-3 mol/l. Unter den geprüften Katalysatoren erwies sich Wolframhexachlorid in Verbindung mit Diethylalumiumethoxid als der geeignetste. Mit diesem System verlief die Polymerisation auch bei großen Monomer/Wolfram-Verhältnissen (10200 :1) hochselektiv und führte zu großen Umsätzen (≥82%).
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  • 90
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The dependence of dilution on the chain propagation and termination rate constants was investigated in the polymerization of ethyl acrylate in benzene solution at 50°C, with the rotating sector method. The errors of the above rate constants were determined and, by our method applied to decrease these errors, the errors of the propagation rate constant was reduced to its half value. By the application of our earlier results in polymerization kinetics, we found that in this system the chain propagation step is exclusively responsible for the solvent effects observed. Our experimental results can be quantitatively described in terms of the hot redical theory.
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  • 91
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    Angewandte Makromolekulare Chemie 185 (1991), S. 329-334 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
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  • 92
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    Angewandte Makromolekulare Chemie 174 (1990), S. 1-10 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Die Photopolymerisation von Ethylenglykoldimethacrylat (EGDMA) mit Methylmethacrylat (MMA) wurde mit Benzoinmethylether (BME) als Initiator in einem Glasrohr durchgeführt, um einen lichtfokussierenden Plastikstab (Radius Rp) mit parabolischer Brechungsindex-Verteilung (Verteilungskonstante A) in der Nähe der Stabachse (bis zum Radius Rc) herzustellen. Die Abhängigkeit von A und Rc/Rp vom Monomerenverhältnis MMA/EGDMA der Ausgangsmischung, der Einfluß der BME-Konzentration auf die Brechungsindex-Verteilung und die Reproduzierbarkeit der Photopolymerisationen wurden untersucht. Das Rc/Rp-Verhältnis stieg mit dem Monomerenverhältnis MMA/EGDMA bei BME = 1,0 Gew.-% und der Geschwindigkeit der UV-Lampe von V = 0,58 mm/min an. Andererseits stieg Rc/Rp bei BME = 0,6 Gew.-% und V = 0,735 mm/min bis zum Monomerenverhältnis von 0,2 an und fiel dann deutlich zwischen 0,20 und 0,25 ab. Die Plastikstäbe, die hier durch Photopolymerisation hergestellt wurden, haben Linseneigenschaften und sind gut reproduzierbar.
    Notes: The photocopolymerization of ethyleneglycol dimethacrylate (EGDMA) with methylmethacrylate (MMA) containing benzoin methyl ether (BME) as initiator was carried out in a glass tube to fabricate a light-focusing plastic rod (radius Rp) with a parabolic refractive index distribution (distribution constant A) in the region near the center axis (radius Rc). The dependence of A and Rc/Rp on MMA/EGDMA feed ratio, influence of BME concentration on the refractive index distribution, and the reproducibilities of the photopolymerizations were investigated. The Rc/Rp increased with the feed ratio used under BME = 1.0 wt.-% at velocity of UV lamp V = 0.58 mm/min. On the other hand, Rc/Rp increased with the feed ratio to 0.2 and then decreased remarkably with the feed ratio in the range of 0.2-0.25 with BME = 0.6 wt.-% at V = 0.735 mm/min. Plastic rods fabricated in the investigation using photopolymerization have lens characteristics and good reproducibilities.
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  • 93
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Polystyrol- und Polymethylmethacrylateinzelfäden mit verschiedenen Graden von Doppelbrechung wurden durch die kontinuierliche Änderung der Ziehgeschwindigkeit, der Temperatur der Polymerschmelze und des Molekulargewichts von Polystyrol (PS) und Polymethylmethacrylat (PMMA) gewonnen. Der “brittle to ductile” Übergangspunkt (Übergang des Polymeren vom spröden zum ziehbaren Zustand) wurde für das optisch reine Polystyrol im Bereich der Doppelbrechungszahlen -0,6 · 10-3 bis -2,6 · 10-3 gefunden. Höhe und Lage dieses Punktes werden durch das Molekulargewicht, die Molekulargewichtsverteilung und die Temperatur der Polymerschmelze beeinflußt. Die Werte des Doppelbrechungsindexes von PS sind um zwei Zehnerpotenzen höher als von PMMA. Bei PMMA wurde der “brittle to ductile” Übergangspunkt nicht beobachtet. Die mechanischen und optischen Eigenschaften hängen nicht nur vom mittleren Wert des durch die Doppelbrechung charakteri-sierten Orientierungsgrades ab, sondern auch davon, welcher Teil des Relaxations-spektrums des Polymeren vorzugsweise orientiert ist. Beim Ziehen der PS- und PMMA-Einzelfäden bilden sich in der Mitte des Fadens Risse (crazes), die jedoch nicht an die Oberflache des Fadens treten.
    Notes: Monofilaments possessing various degrees of birefringence were obtained by changing the drawing rate, the molten polymer temperature, and the molecular weight of polystyrene (PS) and poly(methyl methacrylate) (PMMA). The “brittle-toductile” transition point of optically pure PS was found in the range of birefringences of -0.6 · 10-3 to -2.6 · 10-3. Both the height and position of this point are influenced by M̄w, molecular weight distribution, and polymer melt temperature. The birefringence of PS is higher by two orders of magnitude than that of PMMA in which this transition point has not been observed. The mechanical and optical properties depend not only on the average amount of orientation characterized by the birefringence but on what portion of the relaxation spectrum of the polymer is preferentially oriented. During the drawing of PS and PMMA monofilaments crazes are formed in the centre of the fibers and do not reach the surface.
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  • 94
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    Angewandte Makromolekulare Chemie 174 (1990), S. 105-117 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: The possibility of the application of atactic polypropylene is investigated as graft base for the preparation of graft copolymers of vinyl acetate and vinyl chloride respectively graft copolymers from vinyl acetate onto polypropylene as graft base for the preparation of graft copolymers of vinyl chloride in relation to the composition of the original mixture.After compounding of the graft products with polyvinyl chloride or ABS/polyvinyl-chloride mixtures selected mechanical properties are presented in relation to the quantity and composition of the incorporated graft copolymers.
    Notes: Die Möglichkeiten des Einsatzes von ataktischem Polypropylen als Pfropfgrundlage zur Herstellung von Pfropfcopolymerisaten des Vinylacetates und Vinylchlorids bzw. von Polypropylen-Vinylacetat-Pfropfprodukten als Pfropfgrundlage zur Herstellung von Pfropfcopolymerisaten des Vinylchlorids werden in Abhängigkeit von der Ausgangsgemischzusammensetzung untersucht.Nach Abmischung der Pfropfprodukte mit Polyvinylchlorid bzw. ABS/Polyvinylchlorid-Mischungen werden ausgewählte mechanische Eigenschaften sowohl in Abhängigkeit von der Menge des enthaltenen Pfropfproduktes als auch von der Zusammensetzung der Pfropfpolymerisate dargestellt.
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  • 95
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: A new spectroscopic method for determining the permeability of ammonia in transparent polymer layers is presented. It is based on the quantitative proof of the ammonia passing the test layer into a sensor layer, containing 4-nitrophenol as an NH3-sensitive probe, which is placed under the test film. The permeability (P) of the test layer is calculated from the slope of extinction with time in the sensor layer. The lag time (t1) gives the diffusion coefficient (D). The suitability of this method is confirmed by comparison with literature values. Diffusion coefficients obtained from t1 correspond well to those obtained by the sorption method.The temperature dependence of P and D in more than five different polymers (CDA, PVB, PVAc, PVC and different polyvinyl alcohols) demonstrates that peculiarities in the temperature dependence of the permeability result from the behaviour of the NH3-solubility (P/D) which is strongly dependent on the polymer structure.
    Notes: Es wird eine neuartige spektroskopische Methode zur Messung der Ammoniak-Permeabilität in transparenten Polymerfilmen vorgestellt. Sie beruht auf dem quantitativen Nachweis des den Testfilm durchdringenden Ammoniaks in einer darunter befindlichen Sensorschicht mit Hilfe der NH3-empfindlichen Sonde 4-Nitrophenol. Aus dem Anstieg der Extinktion mit der Zeit (ΔE/Δt) in der Sensorschicht wird die Permeabilität (P) und aus der Verzögerungszeit (lag time (t1)) der Diffusionskoeffizient (D) berechnet. Die Eignung der Methode wird durch Vergleich mit Literaturwerten belegt. Die aus t1 zugänglichen Diffusionskoeffizienten stimmen sehr gut mit denen überein, die mit der Sorptionsmethode gefunden werden.Die Temperaturabhängigkeiten von P und D in mehr als fünf verschiedenen Polymeren (CDA, PVB, PVAc, PVC und in verschiedenen Polyvinylalkoholen) zeigen, daß Besonderheiten im Temperaturgang der Permeabilität aus dem vom Aufbau des Polymeren stark abhängigen Verhalten der NH3-Löslichkeit (P/D) resultieren.
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  • 96
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    Angewandte Makromolekulare Chemie 179 (1990), S. 99-111 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Es wurde die strahleninduzierte Pfropfung von 4-Vinylpyridin (VP) auf Styrol-Butadien-Styrol-Copolymere (SBS) in Gegenwart von gelöstem Sauerstoff untersucht. Pfropfcopolymer-Membranen mit unterschiedlichen Pfropfgraden wurden durch Gießen aus benzolischer Lösung hergestellt. Die mechanischen Eigenschaften, die Gaspermeabilität sowie der Einfluß der Temperatur auf die Gasdurchlässigkeit wurden untersucht. Der höchste Pfropfgrad von 8,4% wurde bei einer Bestrahlungszeit von 15,5 herreicht. Sowohl bei kürzeren als auch längeren Bestrahlungszeiten war der Pfropfgrad  -  bedingt durch Störung durch den vorhandenen Sauerstoff  -  niedriger. Reißfestigkeit und Reißdehnung von SBS-g-VP waren vergleichbar rnit SBS. Die Spannungsrelaxation von SBS-g-VP war langsamer als bei SBS, was auf der Bildung von starren Polyvinylpyridin(DVP)-Mikrophasen beruhen könnte, die als permanente Vernetzungspunkte wirken und die Spannungsrelaxation beeinträchtigen könnten.Da SBS eine hohe Gasdurchlässigkeit und PVP eine hohe O2/N2-Selektivität aufweisen, wurde die Gasdurchlässigkeit der Pfropfmembranen untersucht. Die Selektivitat der SBS-g-VP-Membranen stieg mit steigendem Pfropfgrad, allerdings auf Kosten der Permeabilität, Bei Erhöhung der Temperatur nahm die Durchlässigkeit zu, das Verhältnis O2/N2, aber ab. Die Aktivierungsenergie (Ep) der Gasdurchlässigkeit stieg mit dem Pfropfgrad. Für eine nichtgepfropfte SBS-Membran betrug Ep 5,5 kcal/mol für Sauerstoff und 7,2 kcal/mol für Stickstoff. Für eine SBS-g-VP-Membran mit einem Pfropfgrad von 8,4% waren die Ep-Werte für Sauerstoff und Stickstoff 6,5 bzw. 8,1 kcal/mol.
    Notes: The grafting of 4-vinyl pyridine (VP) onto styrene-butadiene-styrene triblock copolymers (SBS) by homografting irradiation with dissolved oxygen was studied. Homograft membranes of various degree of grafting were prepared from a casting solution of grafted copolymer in benzene. The mechanical properties of membranes, gas permeability, and the effect of operating temperature on gas permeation were investigated.The degree of grafting of 8.4% was the largest at an irradiation time of about 15.5 h. It was smaller at both shorter and longer duration because of the interference of dissolved oxygen. It was found that the tensile strength and elongation of SBS-g-VP were similar to those of SBS. The stress relaxation of SBS-g-VP was slower than that of SBS, and this might be due to the formation of rigid microphase separation domain of poly(4-vinyl pyridine), which acted as permanent crosslinking points to reduce the stress relaxation.Using the properties of high flux of SBS and high O2/N2 selectivity of poly(4-vinyl pyridine), the performance of gas permeation of 4-vinyl pyridine homografted SBS membrane was studied. The selectivity of SBS-g-VP membrane increased with increasing degree of grafting. However, it was done at the expense of a decrease in the gas permeability.When the operating temperature of gas permeation increased, the permeability of oxygen and nitrogen increased, and the O2/N2 permeability ratio decreased. The activation energy (Ep) for gas permeation through different degree of grafting of SBS-g-VP membrane (obtained by the Arrhenius law) increased with increasing degree of grafting. For ungrafted SBS membrane, Ep was 5.5 kcal/mol for oxygen and 7.2 kcal/mol for nitrogen. For 8.4% grafting degree SBS-g-VP membrane, Ep for oxygen and nitrogen, were 6.5 and 8.1 kcal/mol, respectively.
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  • 97
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    Angewandte Makromolekulare Chemie 175 (1990), S. 13-27 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: The change of molecular mass and long-term strength of high and low density polyethylene under tensile load in surface-active medium was studied. It is shown that long-term strength of polyethylene depends on the molecular mass. The influence of polydispersity on strength was investigated.
    Notes: Es wird über die Untersuchungsergebnisse von Lebensdauer und Molmassenänderung an polydispersen Proben von HDPE und HDPE in einem breiten M̄v-Bereich im Zugversuch in einem oberflächenaktiven Medium berichtet.Die Arbeit enthält auch die Darstellung der quantitativen Änderung der Molmasse von Hochdruck- und Niederdruck-Polyethylen unter Dauerbeanspruchung und die Analyse der Lebensdauer in Abhängigkeit von den unterschiedlichen Molmassen (MM) (M̄w, M̄n, M̄v); die Unterschiede in Abhängigkeit von der Lebensdauer von M̄v für Proben und polydisperse Muster sowie die Vorstellungen über den dualen Einfluß der Polydispersität auf die Lebensdauer mit einer Interpretation der auftretenden Erscheinungen werden beschrieben.
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  • 98
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: One constitutive property for the description of dyeing polyester-materials with disperse dyes is the particle size distribution in the dye bath. To measure particle sizes below one micrometer in situ, special techniques are required. One of these is the so called photon-correlation-spectroscopy, depending on the scattering of laser light by suspended small particles in a fluid. The mathematical and physical principles of the photon-correlation-technique are explained in this article.
    Notes: Eine zur Beschreibung des Färbevorganges von Polyestermaterialien mit Dispersionsfarbstoffen wesentliche Größe stellt die Partikelgrößenverteilung im Farbebad dar. Partikelgrößen unterhalb eines Mikrometers lassen sich in situ nur mit wenigen Meßtechniken bestimmen. Dazu zählt u. a. die Photonen-Korrelations-Spektroskopie, die auf Streuung von Laserlicht an kleinen, in einer Flüssigkeit suspendierten Teilchen basiert. Die mathematischen und physikalischen Grundlagen dieser Meßtechnik werden in diesem Artikel erläutert.
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  • 99
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    Angewandte Makromolekulare Chemie 175 (1990), S. 81-97 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Die Kristallinität von Polyethylen, Polyethylenterephthalat und Polypropylen wurde mit Hilfe der Röntgenweitwinkel(WAXS)-und-kleinwinkel(SAXS)streuung gemessen. Die mittels der letzteren Methode erhaltenen Kristallinitätswerte sind deutlich größer als die durch WAXS. Es wird vermutet, daß die Unterschiede mit der Schicht zusammenhängen, die zwischen den amorphen und kristallinen Bereichen liegt. Daraus wurde geschlossen, daß die Röntgenkleinwinkelstreuung nicht als unabhängige Routinemethode zur Kristallinitätsbestimmung angewendet werden kann.
    Notes: The crystallinity of polyethylene, polyethyleneterephthalate, and polypropylene was measured with the aid of wide angle (WAXS) and small angle (SAXS) X-ray scattering methods. The values of crystallinity evaluated using the latter method are distinctly larger than those ones given by the WAXS method. The differences are supposed to be associated with the transition layer existing between the amorphous and crystalline regions. It was concluded that small angle X-ray scattering cannot be employed as an independent routine method of crystallinity determination.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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  • 100
    Electronic Resource
    Electronic Resource
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 175 (1990), S. 117-128 
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Für das System Cellulosenitrat-μ-Styragel-THF wurde die DP-Ve-Beziehung sowie die Ausarbeitung der betreffenden Eichparameter einer genaueren Betrachtung unterzogen. Die etwa dreißig Eichpräparate waren durch definierten säurekatalysierten Abbau von Baumwollcellulose und anschließende Nitrierung hergestellt worden und umfaßten einen DP-Bereich von 150 〈 DP 〈 8000. Es wird gezeigt, daß mit steigendem DP die DP-Ve-Beziehung drei verschiedene Phasen aufweist. Bei der Anwendung der SEC auf Cellulosenitrat müssen daher bezüglich der Eichung zwei Fehlerquellen in Betracht gezogen werden: eine bezieht sich auf die Methode der Ausarbeitung der Eichparameter als solche, und die andere auf die Nichtbeachtung des Vorhandenseins dreier Abschnitte in der DP-Ve-Beziehung. Die Auswirkung dieser Fehler auf die Genauigkeit der aus den Elutionsdiagrammen abgeleiteten Verteilungskurven und DP-Mittelwerte wird diskutiert.
    Notes: The calibration behaviour of the system cellulose nitrate-μ-Styragel-THF has been studied using about 30 different calibration samples prepared by defined acid catalyzed degradation of cotton cellulose. The samples covered a range of DP between 150 〈 DP 〈 8000. It is shown that the DP-Ve relationship is characterized by the existence of three subsequent stages. Consequently, two sources of errors in the DP-Ve calibration must be taken into consideration. One refers to the methods as such to elaborate the calibration parameters, and the other one arises by neglecting that the DP-Ve relationship involves 3 distinct stages. The extent of the two classes of errors in regard to the exactness of the determined molecular weight averages and molecular weight distributions is discussed.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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