Skip to main content
SpringerLink
Log in

Partial purification and characterization of the bone resorption factor fromActinomyces viscosus

  • Published:
Calcified Tissue International Aims and scope Submit manuscript

Summary

The bone resorptive factor and amphipathic antigen (AcA) previously identified by us in preparations fromActinomyces viscosus have been partially purified, characterized chemically, and compared. They elute at the same location on chromatography with Ac 22. The fatty acid composition of AcA and the bone resorptive factor is the same. Some differences in carbohydrate composition are observed. TheActinomyces factor does not affect calcium influx or cyclic AMP in isolated bone cells. Therefore it is concluded that AcA stimulates resorption either by gaining entrance into bone cells or by way of a yet undetermined second messenger.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Schei, O., Waerhaug, J., Lovdal, A., Arno, A.: Alveolar bone loss as related to oral hygiene and age, J. Periodont.30:7–17, 1959

    Google Scholar 

  2. Hausmann, E., Raisz, L. G., Miller, W. A.: Endotoxin: stimulation of bone resorption in tissue culture, Science168:862–864, 1970

    CAS  PubMed  Google Scholar 

  3. Hausmann, E., Weinfeld, N., Miller, W. A.: Effects of lipopolysaccharides on bone resorption in tissue culture, Calcif. Tissue Res.9:272–282, 1972

    Article  CAS  PubMed  Google Scholar 

  4. Hausmann, E., Luderitz, O., Knox, K., Weinfeld, N., Wicken, A.: Structural requirements for bone resorption by endotoxin and lipoteichoic acid, J. Dent. Res.54:B94-B99, 1975

    PubMed  Google Scholar 

  5. Raisz, L. G.: Bone resorption in tissue culture. Factors influencing the response to parathyroid hormone, J. Clin. Invest.44:103–116, 1965

    Article  CAS  PubMed  Google Scholar 

  6. Rowe, D. J., Hausmann, E.: Quantitative analyses of osteoclast changes in resorbing bone organ cultures, Calcif. Tissue Res.23:283–289, 1977

    Article  CAS  PubMed  Google Scholar 

  7. Socransky, S. S., Hubersak, C., Propas, D.: Induction of periodontal destruction in gnotobiotic rats by a human actinomycete, Arch. Oral Biol.15:993–995, 1970

    Article  CAS  PubMed  Google Scholar 

  8. Hausmann, E., Nair, B. C., Reed, M., Levine, M.: Partial characterization of a bone resorptive factor fromActinomyces viscosus, Mechanisms of localized bone loss, Calcif. Tissue Abstr., Inf. Retrieval, Inc., 115–122, 1978

  9. Trummel, C. L., Past, M. J., Cisar, J. O.: A stimulator of bone resorption produced byActinomyces viscosus, J. Dent. Res.56: Sp. Issue B, B156, 1977

  10. Wicken, A. J., Broady, K. W., Evans, J. D., Knox, K. W.: A new cellular and extracellular amphipathic antigen fromActinomyces viscosus NY1. Infect. Immunol.22:615–616, 1978

    CAS  Google Scholar 

  11. Wicken, A. J., Gibbens, J. W., Knox, K. W.: Comparative studies on the isolation of membrane lipoteichoic acid fromLactobacillus fermenti NCTC 6991, J. Bacteriol.113:365–372, 1973

    CAS  PubMed  Google Scholar 

  12. Tauber, H., Garson, W.: Isolation of lipopolysaccharide endotoxin, J. Biol. Chem.234:1391–1393, 1959

    CAS  PubMed  Google Scholar 

  13. Wicken, A. J., Knox, K. W.: Studies on the group F antigen of lactobacilli: isolation of a teichoic acid-lipid complex fromLactobacillus fermenti NCTC 6991, J. Gen. Microbiol.60:293–301, 1970

    CAS  PubMed  Google Scholar 

  14. Knox, K. W., Wicken, A. J.: Serological properties of the wall and membrane teichoic acids fromLactobacillus helveticus NC1B 8025, J. Gen. Microbiol.63:237–248, 1971

    Google Scholar 

  15. Hewett, M. S., Knox, K. W., Wicken, A. J.: Studies on the group F antigen of lactobacilli: detection of antibodies by haemagglutination, J. Gen. Microbiol.60:315–322, 1970

    CAS  PubMed  Google Scholar 

  16. Stern, I., Shapiro, B.: A rapid and simple method for the determination of esterified fatty acids and for total fatty acids in blood, J. Clin. Pathol.6:158–160, 1953

    CAS  PubMed  Google Scholar 

  17. Raisz, L. G., Nieman, I.: Effect of phosphate, calcium, and magnesium on bone resorption and hormonal responses in tissue culture, Endocrinology85:446–456, 1969

    CAS  PubMed  Google Scholar 

  18. Dziak, R., Brand, J. S.: Calcium transport in isolated bone cells. I. Bone cell isolation procedures, J. Cell. Physiol.84:75–84, 1974

    Article  CAS  PubMed  Google Scholar 

  19. Dziak, R., Brand, J. S.: Calcium transport in isolated bone cells. II. Calcium transport studies, J. Cell. Physiol.84:85–96, 1974

    Article  CAS  PubMed  Google Scholar 

  20. Nair, B. C., Hausmann, E.: Bone responsive activity of highly purified lipopolysaccharide fromBacteroides asaccharolyticus, J. Dent. Res.60:Sp. Issue A, 641, 1981

    Google Scholar 

  21. Hausmann, E., Levine, M. J., Chen, P.: Factor(s) fromActinomyces viscosus andnaeslundii which stimulate resorption in bone organ culture, J. Dent. Res.56: Sp. Issue A, A69, 1977

  22. Brown, M., Dziak, R., Hausmann, E.:Actinomyces viscosus amphophile and lipoteichoic acid effects on bone cells, J. Dent. Res.58: Sp. Issue A, 189, 1979

    Google Scholar 

  23. Dziak, R., Hausmann, E., Chang, Y. W.: Effects of lipopolysaccharides and prostaglandins on rat bone cell calcium and cyclic AMP, Arch. Oral Biol.24:347–353, 1979

    Article  CAS  PubMed  Google Scholar 

  24. Dziak, R., Stern, P. H.: Calcium transport in isolated bone cells. III. Effects of parathyroid hormone and cyclic 3′,5′-AMP, Endocrinology97:1281–1287, 1976

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oral Biology, School of Dentistry, State University of New York at Buffalo, 14226, Buffalo, New York, USA

    E. Hausmann, B. C. Nair, M. Brown & R. Dziak

  2. Institute of Dental Research, United Dental Hospital of Sydney, Sydney, Australia

    K. W. Knox

  3. School of Microbiology, University of New South Wales, 2033, Kensington, New South Wales, Australia

    K. W. Broady & A. J. Wicken

Authors
  1. E. Hausmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. C. Nair
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. W. Knox
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. W. Broady
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. J. Wicken
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. Brown
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. R. Dziak
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hausmann, E., Nair, B.C., Knox, K.W. et al. Partial purification and characterization of the bone resorption factor fromActinomyces viscosus . Calcif Tissue Int 34, 49–53 (1982). https://doi.org/10.1007/BF02411208

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02411208

Key words

Search

Navigation