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  • Saccharomyces cerevisiae  (169)
  • temperature  (156)
  • Springer  (324)
  • American Geophysical Union  (1)
  • Irkutsk : Ross. Akad. Nauk, Sibirskoe Otd., Inst. Zemnoj Kory
  • Krefeld : Geologischer Dienst Nordhein-Westfalen
  • München : Bayerisches Landesvermessungsamt
  • 2005-2009  (2)
  • 1985-1989  (323)
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  • 1
    Keywords: Assessment ; Malaria ; Public Health ; Scale ; Weather ; climate change ; public health policy ; temperature
    Description / Table of Contents: Awareness that many key aspects of public health are strongly influenced by climate is growing dramatically, driven by new research and experience and fears of climate change and the research needed to underpin policy developments in area is growing rapidly . This awareness has yet to translate into a practical use of climate knowledge by health policy-makers. Evidence based policy and practice is the mantra of the health sector. If climate scientists are to contribute effectively to health policy at local and global scales then careful empirical studies must be undertaken – focused on the needs of the public health policy and decision-makers. Results presented at the Wengen conference make clear that the science and art of integrating climate knowledge into the control of climate sensitive diseases on a year to year time frame as well as careful assessments of the potential impacts of climate change on health outcomes over longer time frames is advancing rapidly on many fronts. This includes advances in the empirical understanding of mechanisms, methodologies for modeling future impacts, new partnership developments between the health and climate community along with access to relevant data resources, and education and training. In a rapidly evolving field this book provides a snapshot of these emerging themes.
    Pages: Online-Ressource (X, 232 pages)
    ISBN: 9781402068775
    Language: English
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  • 2
    Publication Date: 2021-06-15
    Description: During the A.D. 79 eruption of Vesuvius, Italy, the Roman town of Pompeii was covered by 2.5 m of pyroclastic fall pumice and then partially destroyed by pyroclastic density currents (PDCs). Thermal remanent magnetization measurements performed on the lithic and roof tile fragments embedded in the PDC deposits allow us to quantify the variations in the temperature (Tdep) of the deposits within and around Pompeii. These results reveal that the presence of buildings strongly influenced the deposition temperature of the erupted products. The first two currents, which entered Pompeii at a temperature around 300–360°C, show drastic decreases in the Tdep, with minima of 100–140°C, found in the deposits within the town. We interpret these decreases in temperature as being the result of localized interactions between the PDCs and the city structures, which were only able to affect the lower part of the currents. Down flow of Pompeii, the lowermost portion of the PDCs regained its original physical characteristics, emplacing hot deposits once more. The final, dilute PDCs entered a town that was already partially destroyed by the previous currents. These PDCs left thin ash deposits, which mantled the previous ones. The lack of interaction with the urban fabric is indicated by their uniform temperature everywhere. However, the relatively high temperature of the deposits, between 140 and 300°C, indicates that even these distal, thin ash layers, capped by their accretionary lapilli bed, were associated with PDCs that were still hot enough to cause problems for unsheltered people.
    Description: Published
    Description: B05214
    Description: 4.3. TTC - Scenari di pericolosità vulcanica
    Description: 3.6. Fisica del vulcanismo
    Description: JCR Journal
    Description: reserved
    Keywords: Pompeii ; temperature ; magnetic fabric ; pyroclastic density currents ; 04. Solid Earth::04.08. Volcanology::04.08.08. Volcanic risk
    Repository Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Type: article
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  • 3
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 49-53 
    ISSN: 1476-5535
    Keywords: l-Phenylacetyl carbinol ; Saccharomyces cerevisiae ; Yeast ; Benzaldehyde ; Biotransformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The rate of production ofl-phenylacetyl carbinol bySaccharomyces cerevisiae in reaction mixtures containing benzaldehyde with sucrose or pyruvate as cosubstrate was investigated in short 1 h incubations. The effect of yeast dose rate, sucrose and benzaldehyde concentration and pH on the rate of reaction was determined. Maximum biotransformation rates were obtained with concentrations of benzaldehyde, sucrose and yeast of 6 g, 40 g and 60 g/l, respectively. Negligible biotransformation rates were observed at a concentration of 8 g/l benzaldehyde. The reaction had a pH optimum of 4.0–4.5. Rates of bioconversion of benzaldehyde and selected substituted aromatic aldehydes using both sucrose and sodium pyruvate as cosubstrate were compared. The rate of aromatic alcohol production was much higher when sucrose was used rather than pyruvate.o-Tolualdehyde and 1-chlorobenzaldehyde were poor substrates for aromatic carbinol formation although the latter produced significant aromatic alcohol in sucrose-containing media. Yields of 2.74 and 3.80 g/l phenylacetyl carbinol were produced from sucrose and pyruvate, respectively, in a 1 h reaction period.
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  • 4
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    Journal of industrial microbiology and biotechnology 4 (1989), S. 81-84 
    ISSN: 1476-5535
    Keywords: Ethanol fermentation ; Wheat starch ; Saccharomyces cerevisiae ; immobilization ; Continuous dynamic immobilized biocatalyst bioreactor ; Biocatalyst bioreactor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A simple and efficient method of conversion of wheat starch B to ethanol was investigated. Employing a two-stage enzymatic saccharification process, 95% of the wheat starch was converted to fermentable sugars in 40 h. From 140 g/l total sugars in the feed solution, 63.6 g/l ethanol was produced continuously with a residence time of 3.3 h in a continuous dynamic immobilized biocatalyst bioreactor by immobilized cells ofSaccharomyces cerevisiae. The advantages and the application of this bioreactor to continuous alcoholic fermentation of industrial substrates are presented.
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  • 5
    ISSN: 1570-7458
    Keywords: European corn borer ; Ostrinia nubilalis ; maize ; water ; drought ; stress ; development ; models ; phytotron ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Des plants de maïs se développent dans un phytotron dans 4 conditions d'humidité du sol (de la saturation à la dessication) et à 3 températures constantes (20°, 25° & 30°C). Chaque pied est contaminé au moment de l'émission du pollen, par une ooplaque d'O. nubilalis Hübn. (ECB) de race européenne E. L'installation, la colonisation et le développement des chenilles sont notés lors de 12 périodes de prélèvements destructifs (4 par température). La vitesse de développement d'O. nubilalis est affectée par la température, main non par l'humidité du sol. Les 4 niveaux d'humidité du sol n'ont aucun effet sur la teneur en eau des tiges de maïs. En fait, les feuilles de maïs présentent une senescence précoce lorsqu'il y a déficit en eau dans le sol. La teneur en eau du sol agit sur l'installation, sur la distribution verticale, la dispersion et le lieu d'alimentation des chenilles; mais ces effets sont légers et ne modifient pas la vitesse de développement. L'environnement larvaire dans la tige de maïs est efficacement isolé des variations externes par l'aptitude de la plante à maintenir la teneur en eau des tiges relativement élevée et stable. Ainsi, des changements importants au niveau du sol n'ont pratiquement pas d'effets sur le développement d'O. nubilalis, malgré les conséquences brutales pour la plante. Cette étude montre que la vitesse de développement d'O. nubilalis est relativement insensible aux modifications de la teneur en eau du sol ainsi qu'aux effets de ce stress de sécheresse sévère sur le pied de maïs. La discussion porte sur l'importance de ces résultats pour la modélisation de la dynamique de l'insecte, la physiologie de la culture et les interactions entre insecte et plante.
    Notes: Abstract Maize plants were grown under four moisture regimes (wet to extreme deficit) and three constant temperatures (20°, 25° & 30°C) in a phytotron. Each plant was infested with one E-race European corn borer [Ostrinia nubilalis (Hubn.)] (ECB) egg mass at pollen shed. ECB development, location, and establishment were recorded over the course of 12 destructive sample dates (4/temperature). ECB developmental rates were not significantly affected by soil moisture treatments, but were significantly affected by temperature. In spite of successful establishment of four distinctly different soil moisture regimes, the maize stalk tissue water levels were not significantly different among soil water treatments. Instead, the maize plants exhibited accelerated leaf senescence in response to the water deficit conditions. Among the soil water treatments, differences were found in larval establishment, vertical distribution and dispersion, and feeding site selection; however, those effects were slight and could not explain the similarity in ECB developmental rates observed in these treatments. In maize, the larval environment within the stalk was effectively insulated from changes in the external environment by the plant's ability to maintain a relatively high and stable stalk tissue water content. Thus, large changes to the soil environment had essentially no effect on ECB development, though drastic consequences for the plant. This study indicates that ECB rates of development are relatively insensitive to changes in the soil water environment as well as the associated changes in the maize plant that accompany severe drought stress. The significance of these findings to insect modelling, crop physiology, and insect-crop interactions is discussed.
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  • 6
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    Journal of insect behavior 2 (1989), S. 849-852 
    ISSN: 1572-8889
    Keywords: alternative mating tactics ; temperature ; Stictia heros ; Sphecidae ; Costa Rica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 7
    ISSN: 1572-8773
    Keywords: Manganese ; Electron spin resonance ; Superoxide dismutase ; Saccharomyces cerevisiae ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Manganese accumulation was studied by room-temperature electron spin resonance (ESR) spectroscopy inSaccharomyces cerevisiae grown in the presence of increasing amounts of MnSO4. Mn2+ retention was nearly linear in intact cells for fractions related to both low-molecular-mass and macromolecular complexes (‘free’ and ‘bound’ Mn2+, respectively). A deviation from linearity was observed in cell extracts between the control value and 0.1 mM Mn2+, indicating more efficient accumulation at low Mn2+ concentrations. The difference in slopes between the two straight lines describing Mn2+ retention at concentrations lower and higher than 0.1 mM, respectively, was quite large for the free Mn2+ fraction. Furthermore it was unaffected by subsequent dialyses of the extracts, showing stable retention in the form of low-molecular-mass complexes. In contrast, the slope of the line describing retention of ‘bound’ Mn2+ at concentrations higher than 0.1 mM became less steep after subsequent dialyses of the cell extracts. This result indicates that the macromolecule-bound Mn2+ was essentially associated with particulate structures. In contrast to Cu2+, Mn2+ had no effect on the major enzyme activities involved in oxygen metabolism except for a slight increase of cyanide-resistant Mn-superoxide dismutase activity, due to dialyzable Mn2+ complexes.
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  • 8
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    Cellular and molecular life sciences 45 (1989), S. 171-173 
    ISSN: 1420-9071
    Keywords: Estrogen receptor ; homogenization ; temperature ; nucleus ; uterus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Homogenization of rat uterus at elevated temperatures results in an increased nuclear localization of unoccupied estrogen receptor. This is a nonlinear effect which is accounted for by an increased population of KCl-resistant nuclear binding sites at the elevated homogenization temperatures.
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  • 9
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    Cellular and molecular life sciences 45 (1989), S. 349-351 
    ISSN: 1420-9071
    Keywords: Spermatogenesis ; temperature ; brain ; hibernation ; Helix aspersa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ablation of the brain from hibernatingHelix aspersa maintained at 25°C causes a significant increase in the proliferation of male cells in the gonad, whereas the ablation of the optic tentacles has no effect. The brain, therefore, produces a factor which specifically inhibits the multiplication of spermatogonia and spermatocytes.
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  • 10
    ISSN: 1570-7458
    Keywords: Coleoptera ; Cerambycidae ; Morimus funereus ; development ; laboratory conditions ; food quality ; temperature ; season
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'étude a porté sur les effets d'une température constante (23 °C), de la qualité de l'aliment,-B = aliment naturel, c'est-à-dire de l'écorce de chêne pulvérisée; B+C = aliment naturel enrichi avec de la poudre de biscuits sucrés-, et de la saison sur le développement larvaire de M. funereus L. La température de 23 °C a été défavorable aux larves récoltées en septembre dans des souches de chênes et élevées sur aliment B: les larves étaient mortes en 30 jours. Avec aliment B+C, l'effet défavorable a été neutralisé et le poids des larves augmenté de 543%, 897% et 1179% en 1, 2 et 3 mois. Des larves néonates d'été ou d'hiver se sont bien développées sur B+C, mais toutes les larves d'hiver étaient mortes sur B en 40 j. Des larves de mêmes parents, écloses à différentes époques de l'année, ont présenté des différences saisonnières du taux de survie et de la vitesse de développement lors de leur élevage à 23 °C sur B+C. Le développement le plus rapide et la meilleure survie ont été obtenus avec des larves écloses au début ou au milieu de l'été; tandis que le développement le plus lent et la plus mauvaise survie ont été obtenus avec les larves écloses à la fin de l'été. Les changements saisonniers synchrones des adultes et de leurs descendants, exprimés à différents niveaux d'organisation biologique, suggèrent l'existence d'un rythme annuel endogène qui dépend de l'expression de différents gènes au cours du cycle annuel.
    Notes: Abstract The effects of constant temperature (23 °C), food quality (B, natural diet i.e. powdered oak bark; B+C, natural diet enriched with powdered sweet crackers) and season on larval development of the cerambycid Morimus funereus L. were studied. The temperature of 23 °C exerted an unfavourable effect on larvae collected from oak stumps in September and bred on B; i.e. the larvae died within 30 days. When the larvae consumed B+C the unfavourable effect of temperature was abolished and the larvae increased their weight by 543%, 897% and 1179% in 1, 2 and 3 months, respectively. Newly hatched summerand winter-larvae developed successfully on B+C, while all the winter-larvae died within 40 days on B. Larvae of an identical parentage hatched in different phases of the annual cycle and showed seasonal differences in the rate of development and survival when reared on B+C at 23 °C. The fastest development and the highest survival rate were observed in larvae which emerged in early- and midsummer, whereas the slowest development and the lowest survival rate were in those hatched in late summer. The synchronized seasonal changes of adults and their offspring, as expressed at different levels of biological organization, suggest the existence of an endogenous annual rhythm which is dependent upon the expression of different genes in the course of the annual cycle.
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  • 11
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    Entomologia experimentalis et applicata 52 (1989), S. 185-188 
    ISSN: 1570-7458
    Keywords: Heteroptera ; Dolycoris baccarum ; S. Norwegian population ; adult hibernal diapause ; diapause completion ; diapause activation ; diapause induction ; diapause prevention ; photoperiod ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 12
    ISSN: 1570-7458
    Keywords: Developmental rates ; temperature ; parasitoid ; mealybug ; Hymenoptera ; Encyrtidae ; Epidinocarsis diversicornis ; Acerophagus coccois ; Homoptera ; Pseudococcidae ; Phenacoccus herreni ; cassava ; yucca
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les vitesses de développement des oeufs et de tous les stades larvaires mâles et femelles de P. herreni Cox & Williams ont été déterminées à 18°, 20°, 22°, 25°, 30° et 35°C. La longévité des adultes mâles et femelles a été déterminée. Les vitesses de développement de la ponte à la formation du cocon et de celleci à l'émergence de E. diversicornis Howard, encyrtide parasite de P. herreni ont été déterminées à 18°, 20°, 25° et 30°C. Il en a été de même pour un second encyrtide parasite A. coccois Smith à 20°, 25° et 30°C. Les équations polynomiales des derniers carrés dérivés et les équations logistiques ont été ajustées pour chaque lot de données (à l'exception de A. coccois) de façon à ce qu'elles aient pu être interpolées pour analyser l'impact de ces parasites sur la dynamique de population de P. herreni. Les résultats de cette analyse sont présentés à part.
    Notes: Abstract Developmental rates for Phenacoccus herreni Cox & Williams were determined at 18°, 20°, 22°, 25°, 30° and 35°C for the egg, all juvenile male and all juvenile female stadia. Longevity was determined for adult females and adult males. Developmental rates for the P. herreni parasitoid Epidinocarsis diversicornis (Howard) were determined at 18°, 20°, 25° and 30°C for the oviposition-to-mummy-formation period and the mummy-formation-to-adult-eclosion period. Developmental rates were determined for the P. herreni parasitoid Acerophagus coccois Smith for the same two life stages at 20°, 25° and 30°C. Least-squares-derived polynomial equations or logistic equations were fitted to each data set (except for A. coccois) so that rates could be interpolated for temperatures between observed points for use in an analysis of the impact of these parasitoids on population dynamics of P. herreni. Results of this analysis are presented separately.
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  • 13
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    Entomologia experimentalis et applicata 50 (1989), S. 185-193 
    ISSN: 1570-7458
    Keywords: temperature ; development ; simulation ; phenology ; budmoth ; Zeiraphera canadensis ; Tortricidae ; degree-days
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé La diapause des oeufs de Zeiraphera canadensis Mutuura et Freeman (Lepidoptera: Tortricidae) a été complétée après 15 semaines d'élevage à 0,5°C. A ce stade de leur développement, une température supérieure à 30°C leur est létale. Les relations entre la température et le développement des larves sur l'épinette blanche, Picea glauca (Moench) Voss, et des pupes ont été observées au laboratoire. Les temps de développement les plus courts ont été observés à 28°C. Les larves sont tolérantes à la chaleur et se développent à 32 C, une température qui est létale aux pupes. II y a peu de variabilité des taux de développement de tous les stades immatures. Ceci explique le haut degré de synchronie observé au sein de populations de cet insecte. Un modèle simulant la phénologie a été mis au point et calibré pour prédire la fréquence des divers stades du cycle vital sous des conditions de terrain. La processus de calibration a révélé que la température dans le microhabitat des oeufs est très semblable à celle de l'air, mais que les larves, retrouvées sous l'enveloppe des bourgeons, sont exposées à des températures pouvant excéder celle-ci de plusieurs degrés. Pour des fins de planification du synchronisme entre le développement de l'insecte et des programmes de suivi et de lutte, le nombre de degré-jours nécessaires pour atteindre 10% d'éclosion des oeufs ou 75% d'émergence des adultes ont été estimés à partir d'une température seuil de 4,4°C.
    Notes: Abstract Diapause requirements of eggs of Zeiraphera canadensis Mutuura and Freeman (Lepidoptera: Tortricidae) are satisfied by 15 weeks of storage at 0.5°C. Temperatures above 30°C are lethal for this life stage. Temperature-dependent development of larvae fed on white spruce, Picea glauca (Moench) Voss, and of pupae was observed under laboratory conditions. The shortest development times were observed at 28°C. Larvae are tolerant to heat, and developed at 32°C, a lethal temperature for pupae. There was little variation of development rates in all stages of the life cycle, explaining the high degree of phenological synchrony reported in populations of this species. A phenological model was developed and calibrated to simulate the relative abundance of the various immature stages under field conditions. The calibration process suggested that temperature in the microhabitat of eggs may be very close to air temperature, but that temperatures under bud caps where larvae are feeding may rise several degrees above air temperature. For the purposes of planning the timing of survey and control practices, degree-day requirements for 10% egg hatch and 75% adult emergence were estimated based on a 4.4°C threshold temperature.
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  • 14
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Vector ; Glyphosate resistance ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The E. coli aroA gene was inserted between yeast promoter and terminator sequences in different shuttle expression plasmids and found to confer enhanced EPSP synthase activity as well as resistance to glyphosate toxicity. Subsequently, a transformation system using these newly constructed vectors in yeast was characterized. The efficiency of the glyphosate resistance marker for transformation and selection with plasmid pHR6/20-1 in S. cerevisiae laboratory strain SHY2 was found to be relatively high when compared with selection for LEU2 prototrophy. The fate of the recombinant plasmid pHR6/20-1 in the transformants, the preservation of the aroA E. coli DNA fragment in yeast, mitotic stability, EPSP synthase activity, and growth on glyphosate-containing medium have been investigated. As this plasmid also allows direct selection for glyphosate resistant transformants on rich media, the glyphosate resistance marker was used for transforming both S. cerevisiae laboratory strain SHY2 and brewer's yeast strains S. cerevisiae var. “uvarum” BHS5 and BHS2. In all cases, the vector pHR6/20-1 was maintained as an autonomously replicating plasmid. The resistance marker is, therefore, suitable for transforming genetically unlabeled S. cerevisiae laboratory, wild, and industrial yeast strains.
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  • 15
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Chromosome V ; Ty elements ; tRNA genes ; Transposition hot-spots ; Yeast polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ty4 is a novel transposable element in the yeast, Saccharomyces cerevisiae, which is present in only a few copies in the genome (Stucka et al. 1989). In strain C836 one of the three copies (Ty4-90) is contained in cosmid clone c90, where it resides on chromosome V. Analysis of this region reveals a “hot-spot” of transposition: in addition to Ty4-90, the locus contains a complete Ty3 element and seven singular delta, sigma and tau elements. Three tRNA genes (for His, Lys, and Ile) are located in this region, and these are closely associated with one or the other of the elements, a phenomenon commonly observed in yeast. A comparison of c90 with corresponding regions from other strains shows that the locus is highly polymorphic and that this polymorphism is explicitly associated with Ty transposition and recombination events.
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  • 16
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    Current genetics 16 (1989), S. 315-321 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Antisuppressor ; ADE3 ; Translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutations in a known yeast gene, ADE3, were shown to act as an antisuppressor, reducing the efficiency of the omnipotent suppressor, sup45-2. The ADE3 locus encodes the trifunctional enzyme C1-tetrahydrofolate synthase, which is required for the biosynthesis of purines, thymidylate, methionine, histidine, pantothenic acid and formylmethionyl-tRNAfmet. The role of this enzyme in translational fidelity had not previously been suspected.
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  • 17
    ISSN: 1432-0983
    Keywords: Yeast ; Saccharomyces cerevisiae ; Nonsense suppression ; Omnipotent suppressors ; Gene mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ten dominant omnipotent suppressors of Saccharomyces cerevisiae, which were previously shown to be different from SUP46, have been examined. Nine are mapped in a region between lys5 and cyh2 on the left arm of chromosome VII. These suppressors, like SUP46, manifest sensitivity to increased temperature and the antibiotics paromomycin and hygromycin B. In addition, they have an identical action spectrum. These results strongly suggest that they are allelic to each other and they are designated SUP138. The tenth is mapped to a position between his1 and arg6 on the right arm of chromosome V. This suppressor, named SUP139, does not manifest temperature sensitivity nor antibiotic sensitivity. SUP139 and SUP138, which are clearly distinguished by means of action spectrum, act on much fewer nonsense mutations than SUP46. It is now clear that dominant omnipotent suppressors arising at a single locus are homogeneous and that their efficiency is locus-dependent. The order of efficiency is SUP46〉SUP138〉SUP139.
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  • 18
    ISSN: 1432-0983
    Keywords: Yeast ; Repair ; Complementation ; Saccharomyces cerevisiae ; Schizosaccharomyces pombe ; Gene cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two Saccharomyces cerevisiae genes necessary for excision repair of UV damage in DNA, RAD1 and RAD2, were introduced individually, on a yeast shuttle vector, into seven Schizosaccharomyces pombe mutants — rads1, 2, 5, 13, 15,16 and 17. The presence of the cloned RAD1 gene did not affect survival of any of the S. pombe mutants. The RAD2 gene increased survival of S. pombe rad13 to near the wild-type level after UV irradiation and had no effect on any of the other mutants tested. S. pombe rad13 mutants are somewhat defective in removal of pyrimidine dimers so complementation by the S. cerevisiae RAD2 gene suggests that the genes may code for equivalent proteins in the two yeasts.
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  • 19
    ISSN: 1432-0983
    Keywords: DNA transformation ; Saccharomyces cerevisiae ; Site-specific recombination ; 2μ DNA plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 2μ DNA plasmid of the yeast Saccharomyces cerevisiae does not confer any known selectable phenotype to the host cell carrying it. Selection of cells transformed with purified 2μ DNA therefore cannot be achieved, and the intracellular presence of 2μ can only be assessed by molecular analysis of the DNA complement. In addition, 2μ alone does not replicate in bacterial hosts, thus rendering its amplification by conventional methods impossible. We have isolated a shuttle plasmid, pBH-2L, generated by in vivo sites-pecific recombination between the endogenous 2μ DNA plasmid and pRL, a pBR322 derivative containing the yeast LEU2 gene and one 2μ repeat sequence associated with the origin of replication. This new shuttle plasmid has the property, when transformed into yeast, of undergoing site-specific recombinational resolution between its two direct repeat sequences. This releases 2μ plasmid and pRL as individual molecules. The latter can undergo progressive mitotic loss during growth in nonselective medium, ultimately leaving leucine auxotrophic transformants that contain only 2μ DNA plasmid. This system can be utilized to introduce 2μ DNA alone into cells lacking it, thereby providing a novel means to study the biology and the molecular genetics of the plasmid and its potential practical applications as a vector.
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  • 20
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    Current genetics 15 (1989), S. 113-120 
    ISSN: 1432-0983
    Keywords: Calmodulin mutant ; Nuclear division ; Chromosome stability ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The coding region of a yeast calmodulin gene was fused to a galactose-inducible GAL1 promoter, and a conditional-lethal mutant of Saccharomyces cerevisiae, in which the expression of calmodulin was regulated by galactose, was constructed. The mutant grew normally in galactose medium, but in glucose medium, in which the promoter was repressed, it ceased growing after 12–15 h. The growth arrest was associated with a decrease in intracellular calmodulin levels: after 12h, no intracellular calmodulin protein was detectable. Analysis of the terminal phenotype showed that when the cell stopped growing, it had a bud, a nucleus after S-phase and a short mitotic spindle. Thus, the defect was mainly in nuclear division. Bud growth was partially inhibited in these cells: 27% of the cells stopped growing with a small bud. Furthermore, calmodulin-deficient cells showed elevated rates of chromosome loss, possibly as the result of a defect in the precise segregation of chromosomes.
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    Current genetics 15 (1989), S. 235-237 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Mitochondrial DNA ; Method of extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A rapid method for the extraction of yeast mitochondrial DNA (mtDNA) is described. In comparison with previous methods, it simplifies several steps, does not require either the isolation of mitochondria or phenol treatment and is less time consuming. This protocol gives a high yield of pure mtDNA (50–120 μg from a 100-ml culture), which can be directly used in various molecular applications: restriction enzyme digestion, electrophoresis, blotting, labeling, cloning and sequencing.
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  • 22
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Mutagen hyperresistance ; Southern, Northern analysis ; Gene transplacement ; Transposon mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The genes SNQ and SFA confer hyperresistance to 4-NQO and FA when present on a multi-copy plasmid in yeast. Both are non-essential genes since transplacement of SNQ by a disrupted snq-0::LEU2 yielded stable and viable haploid integrants. Southern analysis revealed that SNQ and SFA are single-loci genes, and OFAGE analysis showed that they are located on chromosome XIII and IV, respectively. Northern blot analysis of SNQ and SFA revealed poly(A)+ RNA transcripts of 2 kb and 1.7 kb, respectively. Nuclease S 1 mapping showed SNQ to have a coding region of 1.6 kb and SFA, one of 1.3 kb. The 5′ coding regions were determined for both genes, while the 3′ end could only be determined for gene SNQ. Both genes do not appear to contain introns. The SFA locus was also mapped by transposon mutagenesis. Tn10-LUK integrants disrupted the SFA gene function at sites that were determined by subcloning to lie within the SFA transcription unit.
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  • 23
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; SKI3 ; SKI5 ; M1 dsRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have identified a mutant strain of the yeast Saccharomyces cerevisiae which overproduces killer toxin. This strain contains a single mutation which fails to complement defects in both the SKI3 and SKI5 genes, while a cloned copy of this gene complements both the ski3 and ski5 defects. The level of secreted toxin from a cDNA based plasmid is not increased in a ski3 strain, showing that the overproduction phenotype is dependent upon an increased level of M1 dsRNA.
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  • 24
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Psoralen photoaddition ; Interstrand cross-link ; Repair deficiency ; Genotoxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two different UVA irradiation systems were initially biologically calibrated with two haploid yeast strains proficient and deficient, respectively, in nucleotide excision repair. The number of DNA lesions introduced into the cell's genome by the photoactivated bifunctional furocoumarin 8-MOP was then calculated by means of the applied UVA exposure doses. At LD37 the repair-proficient wild type had about 14 ICL and 34 furan-side monoadducts in its DNA, while doubly blocked repair mutant rad3-12 pso1-1 had 2 ICL and 3 monoadducts. Locus-specific reversion of lys1-1 followed two-hit kinetics in the repair-proficient wild type and one-hit kinetics in an excision-deficient rad2-20 mutant, as would be expected if ICL was the main type of mutagenic lesion in the wild type and monoadducts the main mutagenic lesion type in the excision-deficient strain. Quantitative comparison of 8-MOP + UVA-induced ICL with those induced by bifunctional mustard revealed the former to have a much higher genotoxicity.
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  • 25
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Protoplast fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The percentage of hybrids formed during protoplast fusion in Saccharomyces cerevisiae is determined by the percentage of protoplasts at the GI/S boundary of the cell cycle.
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  • 26
    ISSN: 1432-0983
    Keywords: Peroxisomes ; Protein import ; Saccharomyces cerevisiae ; Hansenula polymorpha
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    Topics: Biology
    Notes: Summary The DAS gene of Hansenula polymorpha was expressed in Saccharomyces cerevisiae under the control of different promoters. The heterologously synthesized dihydroxyacetone synthase (DHAS), a peroxisomal enzyme in H. polymorpha, shows enzymatic activity in baker's yeast. The enzyme was imported into the peroxisomes of S. cerevisiae not only under the appropriate physiological conditions for peroxisome proliferation (oleic acid media), but also in glucose-grown cells where it induced the enlargement of the few peroxisomes present. This growth process was not accompanied by an increase in the number of microbodies, which suggests a separate control mechanism for peroxisomal proliferation.
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  • 27
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    Current genetics 15 (1989), S. 399-401 
    ISSN: 1432-0983
    Keywords: Saccharomyces exiguus ; Saccharomyces cerevisiae ; HO gene ; MAT gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The DNA of Saccharomyces exiguus was analyzed by Southern hybridization using cloned MATa, MATα, and HO genes of Saccharomyces cerevisiae as probes. It was shown that S. exiguus has a DNA sequence homologous with the HO gene of S. cerevisiae and that this DNA sequence is on a chromosome of about 940 kb of DNA in S. exiguus. However, there is no DNA sequence in S. exiguus that is homologous with the MAT genes of S. cerevisiae.
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  • 28
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    Biodegradation 7 (1989), S. 159-193 
    ISSN: 1572-9729
    Keywords: Brachiopod biogeochemistry ; diagenesis ; secular isotopic variations ; carbon isotopes ; oxygen isotopes ; Sr/Ca ; temperature ; Recent ; Late Paleozoic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sr/Ca ratios in modern brachiopod shells reflect variations in ambient seawater, whereas their Na contents show no relationship with water depth or habitat. Their Mn and Fe contents are controlled, in part, by leaching of these elements from oxide coatings or the low input/sedimentation rate of detrital material into depositional areas such as Quatsino Sound. For most Carboniferous brachiopods from North America, the Mn and Fe contents are similar to those recorded by their Recent counterparts. The high Mn and Fe contents in the brachiopods from shales suggest several possibilities for these levels. One possibility is the leaching of Mn and Fe from oxide coatings/matrix which was not completely removed in the cleaning process, or the high levels in part reflect unusual depositional conditions (some degree of anoxia) for the local shaly environments. The Sr/Ca ratio of brachiopods and, by inference, complementary seawater, did not vary significantly during the Carboniferous. The Sr/Ca minimum observed in brachiopods of Mississippian age coincides with a dip in the 87Sr/86Sr curve and correlates with the Hercynian orogeny. This is attributed to the cycling of seawater through mid-ocean ridge basalts, and postulated exchange reactions account for variation in the composition of seawater-Ca. The unidirectional trend of heavier δ13C values from the Devonian to the Permian is intricately coupled with the evolution of the terrestrial biomass. In addition to expansion of terrestrial plants, burial of reduced carbon in the form of coal (organic matter) contributed to the observed shift. The start of the Permo-Pennsylvanian glaciation is marked by a negative excursion of the secular carbon trend, which is linked to weathering of reduced carbon and its return to the oceanic reservoir with its oxidized carbon. The oxygen isotope values reflect the unidirectional trend towards higher values of the carbon data with decreasing geologic age. Negative excursions of the trend may be related to extensive weathering of terrestrial and submarine rocks, whereas positive excursions may be related to hydrothermal alteration of submarine rocks and dehydration of oceanic crust during times of active sea-floor spreading. Oxygen-calculated water temperatures of unaltered brachiopod material are unrealistically high for all of the Devonian, and the Chesterian-Meramecian, Desmoinesian-Missourian, and Artinskian Epochs. During these times maximum water temperatures of 42° to 56°C are well above the thermal threshold of protein denaturation. This process, which is lethal to most higher organisms, demands an adjustment in oxygen of -2.5%. for samples older than Missourian, and of -1.250%. for samples spanning the Missourian-Artinskian interval. With these adjustments and salinity considerations made prior to calculations, water temperatures become reasonable for the Late Paleozoic epeiric, tropical seas of North America.
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  • 29
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    Cellular and molecular life sciences 45 (1989), S. 102-103 
    ISSN: 1420-9071
    Keywords: Djungarian hamster ; photoperiod ; temperature ; T3 ; T4 ; testosterone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of photoperiod, temperature and testosterone treatment on plasma T3 and T4 levels were investigated in the Djungarian hamster. Plasma T3 level was affected by temperature (25°C〈7°C) but not by photoperiod. Plasma T4 level was affected by photoperiod (short day 〈 long day) at 25°C. Administration of testosterone increased plasma T4 level under short photoperiod at 25°C. Thus, higher plasma T4 level under long photoperiod at 25°C might be induced by testosterone.
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  • 30
    ISSN: 1420-9071
    Keywords: Saccharomyces cerevisiae ; growth conditions ; kinaseless mutant ; plasma membrane vesicles ; glucose transport ; kinetics and computer simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In this study experimental data on the kinetic parameters investigated by other authors1–5, 11 together with own data on plasma membrane vesicles, have been subjected to a computer simulation based on the equations describing facilitated diffusion. The simulation led to an ideal fit describing the above data. From this it can be concluded that glucose is transported by facilitated diffusion, and not by active transport as was postulated by Van Steveninck14, 15. The simulation method also demonstrates that the fast sampling technique used by these authors1–5,11 underestimates the fluxes. Thus, the parameters given do not contribute to the understanding of glucose transport under different metabolic conditions. The K value of plasma membrane vesicles prepared from glucose-repressed cells is around 7 mM. Derepression, particularly by galactose, causes a highly significant increase in affinity as shown by a decrease in the K value to 2 mM. The highest affinity was measured in a triple kinaseless mutant grown on glycerol with a K value of 1 mM. If seems, therefore, that the kinetic parameters derived from initial uptake rates of glucose in intact cells1–5,11 using single flux analysis, such as Eadie-Hofstee- or Lineweaver-Burk-plots, are in error.
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  • 31
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Yeast ; Phospholipase B ; Lysophospholipase ; Enzyme inhibition ; AMP ; Unesterified fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Divalent cations activate the lysophospholipase and transacylase reactions catalyzed by the same enzymes in the yeast Saccharomyces cerevisiae. The activation was observed at neutral pH, but not at the pH optimum of lysophospholipase/transacylase, near 3.5. Adenine nucleotides, especially AMP and ADP, are strong inhibitors of the same group of enzymes. Half maximal inhibition by AMP was found at a concentration of about 20 μM. The inhibition by nucleotides in low concentrations is enhanced by divalent cations.
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    Archives of microbiology 151 (1989), S. 198-202 
    ISSN: 1432-072X
    Keywords: Sexual agglutination ; Mating ; Saccharomyces cerevisiae ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic regulation of the inducibility of sexual agglutination ability in the yeast Saccharomyces cerevisiae was studied. Detailed analysis of the degree of sexual agglutination was carried out; it showed that a greater number of genes are involved in the regulation of inducible sexual agglutination in strain H1-0 than previously assumed. Although dominancy of inducible phenotype over constitutive was confirmed, the effectiveness of one gene changing the constitutive phenotype to the inducible seemed to be somewhat low. Quantity per cell of agglutination substances responsible for sexual agglutination increased as the agglutination ability became greater.
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  • 33
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    Archives of microbiology 151 (1989), S. 391-398 
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Exoglucanases ; Purification ; Protein moieties ; Tunicamycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Exoglucanase (exo-1,3-β-D-glucan glycohydrolase, EC 3.2.1.56) activity secreted by Saccharomyces cerevisiae into the culture medium was separated by ion exchange chromatography into two glycoprotein isoenzymes which contributed 10% (exoglucanase I) and 90% (exoglucanase II) towards the total activity. Analysis of the “in vitro” deglycosylated products by polyacrylamide gel electrophoresis under native or denaturing conditions indicated that the protein portions of both exoglucanases exhibited identical mobility, each one consisting of two polypeptides with M r of 47000 and 48000. The same profile was shown by the exoglucanase secreted in the presence of tunicamycin. Antibodies raised against the protein portion of exoglucanase II did react with both native exoglucanases and their deglycosylated products with a pattern indicative of immunological identity. Digestion of the “in vitro” deglycosylated products of both exoglucanases with Staphylococcus aureus V-8 protease or trypsin generated the same proteolytic fragments in each case. Only exoglucanase II was secreted by protoplasts. These and previously reported results indicate that the protein portions of both isoenzymes may be the product of the same gene (or a family of related genes), and that exoglucanase I is a product of enzyme II, modified by a process occurring beyond the permeability barrier of the cell.
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  • 34
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Recombination ; Tryptophan cluster ; Yeast vectors ; Plasmid copy number
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We describe a convenient method for the in vivo construction of large plasmids that possess a multitude of restriction sites. A large (23 kbases) circular self-replicating plasmid carrying a partial LEU2-d gene was cotransformed with a circular non-replicating plasmid carrying the entire LEU2 gene. In vivo recombination results preferentially in a plasmid that carries both the LEU2-d and the entire LEU2 gene. In addition we also found one plasmid with a tandem LEU2 insertion and one plasmid where the LEU2-d gene was replaced by the entire LEU2 gene.
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  • 35
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    The journal of membrane biology 112 (1989), S. 277-289 
    ISSN: 1432-1424
    Keywords: myelinated nerve fiber ; gating current ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Asymmetrical displacement currents and Na currents of single myelinated nerve fibers ofXenopus laevis were studied in the temperature range from 5 to 24°C. The time constant of the on-response atE=4 mV,τ on, was strongly temperature dependent, whereas the amount of displaced charge atE=39 mV, Qon, was only slightly temperature dependent. The mean Q10 forτ on -1 was 2.54, the mean Q10 for Qon was 1.07. The time constant of charge immobilization,τ i , atE=4 mV varied significantly (α=0.001) with temperature. The mean Q10 forτ i -1 was 2.71±0.38. The time constants of immobilization of gating charge and of fast inactivation of Na permeability were similar in the temperature range from 6 to 22°C. The Qoff/Qon ratio forE=4 mV pulses of 0.5 msec duration decreased with increasing temperature. The temperature dependence of the time constant of the off-response could not be described by a single Q10 value, since the Q10 depended on the duration of the test pulse. Increasing temperature shifted Qon (E) curves to more negative potentials by 0.51 mVK −1, but shiftedP Na (E) curves andh ∞ (E) curves to more positive potentials by 0.43 and 0.57 mV K−1, respectively.h ∞ (E=−70 mV) increased monotonously with increasing temperature. The present data indicate that considerable entropy changes may occur when the Na channel molecule passes from closed through open to inactivated states.
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  • 36
    ISSN: 1617-4623
    Keywords: Meiosis ; Saccharomyces cerevisiae ; Sporulation ; Inessential genes ; Meiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary SPR3 is one of at least nine genes which are expressed in sporulating Saccharomyces cerevisiae cells at the time of meiosis I. We show below that strains homozygous for null alleles of SPR3 are capable of normal meiosis and the production of viable ascospores. We have also monitored SPR3 expression in a series of strains that are defective in meiotic development, using an SPR3: lacZ fusion carried on a single copy plasmid. β-Galactosidase activity occurred at wild-type levels in diploid strains homozygous for mutations in spo13, rad50, rad57 and cdc9, but was greatly reduced in strains carrying cdc8 or spo7 defects. We conclude that SPR3 expression is a valid monitor of early meiotic development, even though the gene is inessential for the sporulation process.
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  • 37
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    Molecular genetics and genomics 217 (1989), S. 149-154 
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; HOM2 gene ; Aspartic semi-aldehyde ; General control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Saccharomyces cerevisiae the HOM2 gene encodes aspartic semi-aldehyde dehydrogenase (ASA DH). The synthesis of this enzyme had been shown to be derepressed by growth in the presence of high concentrations of methionine. In the present work we have cloned and sequenced the HOM2 gene and found that the promoter region of this gene bears one copy of the consensus sequence for general control of amino acid synthesis. This prompted us to study the regulation of the expression of the HOM2 gene. We have found that ASA DH is the first reported enzyme of the related threonine and methionine pathway to be regulated by the general control of amino acid synthesis.
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  • 38
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    Molecular genetics and genomics 217 (1989), S. 464-470 
    ISSN: 1617-4623
    Keywords: Nucleosome positioning ; LEU2 gene ; Saccharomyces cerevisiae ; tRNA3 Leu gene ; Chromatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chromatin structure of theLEU2 gene and its flanks has been studied by means of nuclease digestion, both with micrococcal nuclease and DNase I. The gene is organized in an array of positioned nucleosomes. Within the promoter region, the nucleosome positioning places the regulatory sequences, putative TATA box and upstream activator sequence outside the nucleosomal cores. The tRNA3 Leu gene possesses a characteristic structure and is protected against nucleases. Most of the 5′ flank is sensitive to DNase I digestion, although no clear hypersensitive sites were found. The chromatin structure is independent of either the transcriptional state of the gene or the chromosomal or episomal location. Finally, in the plasmid pJDB207, which lacks most of the promoter, we have found that the chromatin structure of the coding region is similar to that of the wild-type allele.
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  • 39
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    Molecular genetics and genomics 216 (1989), S. 511-516 
    ISSN: 1617-4623
    Keywords: Nonsense mutation ; Read-through ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The yeast invertase structural gene SUC2 has two naturally occurring alleles, the active one and a silent allele called suc2°. Strains carrying suc2° are unable to ferment sucrose and do not show detectable invertase activity. We have isolated suc2° and found an amber codon at position 232 of 532 amino acids. However, transformants carrying suc2° on a multicopy plasmid were able to ferment sucrose and showed detectable invertase activity. Full-length invertase was found in gels stained for active invertase and in immunoblots. Therefore we concluded that the amber codon is occasionally read as an amino acid. The calculated frequency of read-through is about 4% of all translation events.
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  • 40
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    Molecular genetics and genomics 218 (1989), S. 293-301 
    ISSN: 1617-4623
    Keywords: Sporulation ; DNA sequence ; Saccharomyces cerevisiae ; Meiosis ; Chromosome segregation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic studies have previously demonstrated that the RED1 gene of Saccharomyces cerevisiae is required for chromosome segregation at the first meiotic division. Northern blot hybridization analysis indicates that the RED1 gene produces two transcripts of 2.75 and 3.2 kilobases. The major 2.75 kb transcript is not present in mitotic cells and is meiotically induced to accumulate maximally just prior to the meiosis I division. The DNA sequence of the RED1 gene was determined and used to predict the amino acid sequence of the encoded gene product. The RED1 protein is 827 amino acids in length and has a molecular weight of 95.5 kilodaltons. There is no significant homology between the RED1 amino acid sequence and other known protein sequences, including those encoded by genes essential for meiosis.
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  • 41
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    Molecular genetics and genomics 215 (1989), S. 401-406 
    ISSN: 1617-4623
    Keywords: Protein secretion ; Saccharomyces cerevisiae ; Invertase ; Endoplasmic reticulum ; Secretion mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intercompartmental transport of secreted proteins in yeast was analysed using invertase mutants. Deletions and insertions at the BamHI (position +787) or the Asp718 (position +1159) sites of the SUC2 gene led to mutant proteins with different behaviour regarding secretion, localization and enzyme activity. The deletion mutants showed accumulation of core glycosylated material in the endoplasmic reticulum (ER) a decrease of secreted protein by 5%–30% and loss of enzyme activity. The secreted material was localized in the culture medium and not — as is normal for invertase-in the cell wall. No delay in transport from the Golgi to the cell surface was observed, indicating that the rate-limiting step for secretion is at the ER-Golgi stage. Two insertion mutants, pIPA and pIPB, retained enzyme activity. Mutant pIPB showed 10% secretion, while 60%–70% secretion was observed for pIPA. While the non-secreted material accumulated in the ER, the secreted material was present in the cell wall. The results suggest that the presence of structures incompatible with secretion leads to ER accumulation of mutated invertase.
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  • 42
    ISSN: 1617-4623
    Keywords: RNA splicing ; Maturase ; Recombinase ; Mitochondria ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When the bI4 RNA maturase, encoded by the fourth intron of the mitochondrial cytochrome b gene of Saccharomyces cerevisiae, was expressed in Escherichia coli, formation of intra-chromosomal Lac+ recombinants was stimulated threefold. This “hyper-rec” phenotype was recA as well as recBCD dependent. The most active form of the bI4 maturase stimulated homologous recombination whereas splicing deficient mutants of bI4 maturase were either deficient in or unable to stimulate homologous recombination.
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  • 43
    ISSN: 1617-4623
    Keywords: Nucleotide sequence ; PET gene ; Mitochondrial import ; Saccharomyces cerevisiae
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    Notes: Summary Translation of mitochondrial cytochrome b mRNA in yeast is activated by the product of the nuclear gene CBS1. CBS1 encodes a 27 kDa precursor protein, which is cleaved to a 24 kDa mature protein during the import into isolated mitochondria. The sequences required for mitochondrial import reside in the amino-terminal end of the CBS1 precursor. Deletion of the 76 amino-terminal amino acids renders the protein incompetent for mitochondrial import in vitro and non-functional in vivo. When present on a high copy number plasmid and under the control of a strong yeast promoter, biological function can be restored by this truncated derivative. This observation indicates that the CBS1 protein devoid of mitochondrial targeting sequences can enter mitochondria in vivo, possibly due to a bypass of the mitochondrial import system.
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  • 44
    ISSN: 1617-4623
    Keywords: Mitochondrial introns ; Reverse transcriptase ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Some pet- (or mit-) mutations impeding the splicing of one or several intron(s) of the yeast mitochondrial pre-mRNA(s) are suppressed in vivo by the DNA deletion of these introns. We have genetically demonstrated that introns aI1 and/or aI2 of the cytochrome c oxidase subunit 1 gene are necessary for this deletion process. The facts that adjacent introns are simultaneously deleted and that, in the pet- (or mit-) mutants which easily revert by intron deletion, the splicing of the introns they affect is only partially blocked, suggest that the intron encoded proteins aI1 and/or aI2 could intervene by means of their putative reverse transcriptase activity.
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  • 45
    ISSN: 1615-6102
    Keywords: Microtubule neoformation ; Nocodazole ; Protoplasts ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary By following microtubule neoformation after their complete destruction by nocodazole, we analyzed the pattern of microtubule nucleation in protoplasts ofSaccharomyces cerevisiae. Using immunofluorescence, the drug was shown to induce rapid and complete disassembly of both cytoplasmic and spindle microtubules and to selectively block protoplast nuclear division at a defined stage of the cell cycle. Treated protoplasts placed in a drug-free environment recovered a more abundant microtubular system. The majority of microtubules re-formed at SPBs whereas a minority of free-ended microtubules nucleated in the cytoplasm of the protoplasts without any detectable association with recognizable nucleation sites. Random nucleation of free microtubules might be induced by high amounts of unpolymerized tubulin likely to be present in the protoplasts at the moment of drug release.
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  • 46
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    Journal of applied phycology 1 (1989), S. 59-65 
    ISSN: 1573-5176
    Keywords: temperature ; acclimation ; Eucheuma alvarezii ; Eucheuma isiforme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Branch cultures ofEucheuma alvarezii Doty var.tambalang Doty, farmed in the Philippines, andE. isiforme (C. Agardh) J. Agardh var.denudatum Cheney, from the west coast of Florida, were gradually transferred through three temperature regimes over a 6-week period. Photosynthetic responses were measured under a series of irradiances (P-I curves) and temperatures to determine immediate responses of the plants before, during and after completion of the transfers. The Philippine variety did not show acclimation to 18 °C either after gradual transfer from the initial culture temperature of 25 °C or when abruptly transferred from 25 to 18 °C. The Florida variety did show acclimation to 25 °C when gradually transferred from 18 to 22 to 25 °C over the 6-week period, but not if abruptly transferred from 18 to 25 °C. The west coast variety ofE. isiforme from Florida shows a temperature acclimation ability that parallels the seasonal changes in water temperature of its habitat.
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    Plant and soil 119 (1989), S. 87-97 
    ISSN: 1573-5036
    Keywords: irradiance ; root development ; root growth ; shoot development ; shoot growth ; temperature ; thermal time ; winter wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat plants were grown in columns of soil until early stem elongation at a wide range of constant root temperatures. Two light environments were imposed and three levels of nitrogen fertilizer added at sowing. Shoot and root development and growth were measured by destructive sampling to investigate the combined effects of temperature and changing nutrient and assimilate supply. Both mainstem leaf and root axis production were linearly related to thermal time above a base temperature of 0°C. Low irradiance affected the appearance of mainstem tillers and associated nodal root axes. Nitrogen had little effect on shoot or root development but increased shoot area between 6 and 8 mainstem leaves. Higher temperatures and supplementary light resulted in larger root systems when compared at equivalent times after sowing. Total root length and root dry weight increased exponentially with thermal time, based on the mean of 4 cm soil and 2 cm air temperatures, but no single relation existed for all temperature and light treatments. Total plant dry matter, root length and root dry weight increased linearly with accumulated, intercepted, photosynthetically active radiation. Root growth responded less than the shoot to supplementary light. Increasing temperature reduced the proportion of root weight to total plant weight.
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    Plant and soil 119 (1989), S. 99-110 
    ISSN: 1573-5036
    Keywords: Irradiance ; root development ; root growth ; temperature ; thermal time ; winter wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Winter wheat was sown on 2 dates with 3 levels of nitrogen fiertiliser (0, 50 and 200 kg N ha−1) in one year and on 2 sites in a followign season. Shoot and root development and growth were measured between emergence and anthesis in the first season and emergence and 7 mainstem leaves in the second. Differences in temperature and light regime led to significant differences in shoot and root development and growth between sowing dates. A thermal time-scale, based on soil surface or air temperatures, with a base of 0°C, adequately described the production of mainstem leaves and nodal root axes over all treatments. Autumn applied nitrogen had little effect on development. Shoot growth and green area index increased exponentially with thermal time prior to spring nitrogen application and the completion of canopy development. Early-sown crops had larger root systems than late-sown crops prior to winter and this divergence was retained until anthesis. The relationship between root growth and thermal time was little better than with days after sowing and was not improved by either varying the site of temperature measurement or the base temperature used for calculation. Differences in soil texture and drainage, between sites, led to significant changes in root length distribution. Although spring applied nitrogen generally increased root length, its effects were inconsistent. There was a curvilinear relation between root length and the amount of photosynthetically active radiation (PAR) intercepted; this relation was unaffected by sowing date or nitrogen treatment. The amount of root produced per unit PAR decreased as the season progressed, reflecting the decrease in the proportion of total dry matter partitioned to the root system.
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  • 49
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    Molecular genetics and genomics 218 (1989), S. 531-535 
    ISSN: 1617-4623
    Keywords: Origin of replication ; Promoter ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In an attempt to devise a new assay for ARS-binding proteins we have inserted the HO ARS between the upstream activation site and the TATA region of the yeast CYC1 promoter. A marked reduction in promoter activity is observed. Inactivation of the HO ARS element by point mutation does not restore promoter activity to its original level, although a modest activation is seen. We have also inserted the HO ARS into the intron of the yeast actin gene; although there is no apparent deleterious effect on transcription, the activity of the ARS is abolished in this new environment.
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  • 50
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; Heavy metal resistance ; DNA sequence ; Membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A DNA fragment conferring resistance to zinc and cadmium ions in the yeast Saccharomyces cerevisiae was isolated from a library of yeast genomic DNA. Its nucleotide sequence revealed the presence of a single open reading frame (ORF; 1326 bp) having the potential to encode a protein of 442 amino acid residues (molecular mass of 48.3 kDa). A frameshift mutation introduced within the ORF abolished resistance to heavy metal ions, indicating the ORF is required for resistance. Therefore, we termed it the ZRC1 (zinc resistance conferring) gene. The deduced amino acid sequence of the gene product predicts a rather hydrophobic protein with six possible membrane-spanning regions. While multiple copies of the ZRC1 gene enable yeast cells to grow in the presence of 40 mM Zn2+, a level at which wild-type cells cannot survive, the disruption of the chromosomal ZRC1 locus, though not a lethal event, makes cells more sensitive to zinc ions than are wild-type cells.
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  • 51
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; a-factor ; Conjugation ; G1 arrest ; ssl mutations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Nine independent mutants which are supersensitive (ssl −) to G1 arrest by the mating hormone a-factor were isolated by screening mutagenized Saccharomyces cerevisiae MATα cells on solid medium for increased growth inhibition with a-factor. These mutants carried lesions in two complementation groups, ssl1 and ssl2. Mutations at the ssl1 locus were mating type specific: MATα ssl1 − cells were supersensitive to α-factor but MATα ssl1 − were not supersensitive to α-factor. In contrast, mutations at the ssl2. locus conferred supersensitivity to the mating hormone of the opposite mating type on both MATα, and MATa cells. The α-cell specific capacity to inactivate externally added a-factor was shown to be lacking in MATα ssl1 − mutants whereas MATα ssl2. cells were able to inactivate a-factor. Complementation analysis showed that ssl2 and sst2, a mutation originally isolated as conferring supersensitivity to α-factor to MATa cells, are lesions in the same gene. The ssl1 gene was mapped 30.5 centi-Morgans distal to ilv5 on chromosome XII.
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  • 52
    ISSN: 1617-4623
    Keywords: Aspergillus oryzae ; Alkaline protease ; Prepro sequence ; Heterologous expression ; Saccharomyces cerevisiae
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    Topics: Biology
    Notes: Summary We have cloned and determined the nucleotide sequence of a cDNA fragment for the entire coding region of the alkaline protease (Alp) from a filamentous ascomycete Aspergillus oryzae. According to the deduced amino acid sequence, Alp has a putative prepro region of 121 amino acids preceding the mature region, which consists of 282 amino acids. A consensus sequence of a signal peptide consiting of 21 amino acids is found at the N-terminus of the prepro region. The primary structure of the mature region shares extensive homology (29%–44%) with those of subtilisin families, and the three residues (Asp 32, His 64 and Ser 221 in subtilisin BPN′) composing the active site are preserved. The entire cDNA, coding for prepro Alp, when introduced into the yeast Saccharomyces cerevisiae, directed the secretion of enzymatically active Alp into the culture medium, with its N-terminus and specific activity identical to native Aspergillus Alp.
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  • 53
    ISSN: 1617-4623
    Keywords: Gene conversion ; Crossing-over ; Mismatch repair ; Saccharomyces cerevisiae ; Psoralens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The induction of gene conversion and mitotic crossing-over by photoaddition of psoralens, 254 nm ultraviolet radiation, and nitrogen mustards was determined in diploid cells homozygous for the pso3-1 mutation and in the corresponding wild type of Saccharomyces cerevisiae. For these different agents, the frequency of non-reciprocal events (conversion) is reduced in the pso3-1 mutant compared to the wild type. In contrast, the frequency of reciprocal events (crossing-over) is increased at a range of doses. These observations, together with the block in induced mutagenesis for both reverse and forward mutations previously reported for the pso3-1 mutant, suggest that the PS03 gene product plays a role in mismatch repair of short patch regions. The block in gene conversion in the pso3 homozygous diploid leads, in the case of nitrogen mustards, to specific repair intermediates which are lethal to the cells.
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  • 54
    ISSN: 1617-4623
    Keywords: Enhanced secretion ; Human lysozyme production ; Protease mutant ; Protein processing ; Saccharomyces cerevisiae
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    Topics: Biology
    Notes: Summary Yeast mutant strains which secrete large amounts of human lysozyme were screened using an agar medium containing bacterial cells. Nine mutants secreted over 10 times more lysozyme than the wild-type parent strain. The mRNA levels for lysozyme in the mutants were not higher than that of the wild-type strain. Three of the mutant strains were deficient in carboxypeptidase Y activity. It was found that the protease deficiency was caused by a deficiency in conversion of proenzyme to mature enzyme in ssl1 mutant cells. The ssl1 gene was found to be closely linked to the centromere and determine both the efficiency of secretion of lysozyme and the processing of carboxypeptidase Y.
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  • 55
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    Molecular genetics and genomics 219 (1989), S. 495-498 
    ISSN: 1617-4623
    Keywords: ARS1 ; Plasmid multimerization ; RAD52 ; Saccharomyces cerevisiae ; Eukaryotic recombination
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    Topics: Biology
    Notes: Summary A mutant plasmid, pX, derived from the 1453 base pair small plasmid, YARp1 (or TRP1 RI circle), consists of 849 base pairs of DNA bearing the TRP1 gene and the ARS1 sequence of Saccharomyces cerevisiae and, unlike YARp1 and other commonly used yeast plasmids, highly multimerizes in a S. cerevisiae host. The multimerization of pX was dependent on RAD52, which is known to be necessary for homologous recombination in S. cerevisiae. Based upon this observation, a regulated system of multimerization of pX with GAL1 promoter-driven RAD52 has been developed. We conclude that the regulated multimerization of pX could provide a useful model system to study genetic recombination in the eukaryotic cell, in particular to investigate recombination intermediates and the effects of various trans-acting mutations on the multimerization and recombination of plasmids.
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  • 56
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    Molecular genetics and genomics 215 (1989), S. 455-462 
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; Orotate phosphoribosyl transferase ; Nucleotide sequence ; Transcription ; Regulation
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    Topics: Biology
    Notes: Summary The URA5 gene of Saccharomyces cerevisiae encodes orotate phosphoribosyl transferase (EC 2.4.2.10; OPRTase) which catalyses the transformation of orotate to OMP in the pyrimidine pathway. We present in this paper the cloning and the sequencing of this gene, the last in the yeast pyrimidine pathway to be cloned. We have deduced the protein sequence of the OPRTase of S. cerevisiae from the DNA sequence and compared it to that of Escherichia coli, Podospora anserina and Dictyostelium discoideum. Some important similarities in the structure of these four proteins have been found. Finally, we have quantified the transcription of the URA5 gene in different physiological conditions and confirmed that it was not under the control of UTP or any intermediary product of the pathway.
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  • 57
    ISSN: 1617-4623
    Keywords: DNA sequence ; PET gene ; Saccharomyces cerevisiae ; Mitochondrial import ; cytochrome c oxidase
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    Topics: Biology
    Notes: Summary The yeast nuclear SCO1 gene is required for accumulation of the mitochondrially synthesized cytochrome c oxidase subunits I and II (COXI and COXII). We cloned and characterized the SCO1 gene. It codes for a 0.9 kb transcript. DNA sequence analysis predicts a 33 kDa protein. As shown by in vitro transcription and translation experiments in combination with import studies on isolated mitochodria, this protein is matured into a 30 kDa polypeptide which is tightly associated with a mitochondrial membrane. The possible function of the SCO1 gene product in the assembly of cytochrome c oxidase is discussed.
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  • 58
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; GLN1 ; Glutamine synthetase ; Regulatory systems ; Transcription
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    Topics: Biology
    Notes: Summary TheGLN1 gene ofSaccharomyces cerivisiae was cloned by complementation of agln1 auxotroph. AGLN1-lacZ fusion was constructed to assayGLN1 promoter activity. β-Galactosidase and glutamine synthetase expression in chromosomally integratedGLN1-lacZ fusion strains were co-regulated in response to a shift from glutamine to glutamate as the nitrogen source, purine limitation, and 3-aminotriazole-induced histidine starvation. Regulation ofGLN1 expression by each of the three pathways occurred at the transcriptional level. Increased accumulation ofGLN1 mRNA was observed within 5 min after a shift from glutamine to glutamate as the nitrogen source. After 5 min following glutamine addition to the cells growing with glutamate as nitrogen source. This indicates that theGLN1 message is unstable and has a half-life of approximately 3 min. Deletion analysis indicated that the sequences required forGLN1 expression are located within approximately 350 bp upstream from the transcriptional initiation site.
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  • 59
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; DNA repair ; Cross-link ; Transposon mapping ; Nitrogen mustard
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    Notes: Summary We have isolated yeast gene SNM1 via complementation of sensitivity towards bi- and tri-functional alkylating agents in haploid and diploid yeast DNA repair-deficient snm1-1 mutants. Four independent clones of plasmid DNA containing the SNM1 locus were isolated after transformation with a YEp24-based yeast gene bank. Subcloned SNM1-containing DNA showed (i) complementation of the repair-deficiency phenotype caused by either one of the two different mutant alleles snm1-1 and snm1-2 ts; (ii) complementation in haploid and diploid yeast snm1-1 mutants by either single or multiple copies of the SNM1 locus; and (iii) that the SNM1 gene is at most 2.4 kb in size. Expression of SNM1 on the smallest subclone, however, was under the control of the GAL1 promotor. Gene size and direction of transcription was further verified by mutagenesis of SNM1 by Tn10-LUK transposon insertion. Five plasmids containing Tn10-LUK insertions at different sites of the SNM1-containing DNA were able to disrupt function of genomic SNM1 after gene transplacement. Correct integration of the disrupted SNM1::Tn10-LUK at the genomic site of SNM1 was verified via tetrad analysis of the sporulated diploid obtained after mating of the SNM1::Tn10-LUK transformant to a haploid strain containing the URA3 SNM1 wild-type alleles. The size of the poly(A)+ RNA transcript of the SNM1 gene is 1.1 kb as determined by Northern analysis.
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  • 60
    ISSN: 1617-4623
    Keywords: pso4-1 mutation ; Saccharomyces cerevisiae ; Error-prone recombinational repair ; Mitotic recombination
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    Topics: Biology
    Notes: Summary The induction of mitotic gene conversion and crossing-over inSaccharomyces cerevisiae diploid cells homozygous for thepso4-1 mutation was examined in comparison to the corresponding wild-type strain. Thepso4-1 mutant strain was found to be completely blocked in mitotic recombination induced by photoaddition of mono- and bifunctional psoralen derivatives as well as by mono- (HN1) and bifunctional (HN2) nitrogen mustards or 254 nm UV radiation in both stationary and exponential phases of growth. Concerning the lethal effect, diploids homozygous for thepso4-1 mutation are more sensitive to all agents tested in any growth phase. However, this effect is more pronounced in the G2 phase of the cell cycle. These results imply that the ploidy effect and the resistance of budding cells are under the control of thePSO4 gene. On the other hand, thepso4-1 mutant is mutationally defective for all agents used. Therefore, thepso4-1 mutant has a generalized block in both recombination and mutation ability. This indicates that thePSO4 gene is involved in an error-prone repair pathway which relies on a recombinational mechanism, strongly suggesting an analogy between thepso4-1 mutation and theRecA orLexA mutation ofEscherichia coli.
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  • 61
    ISSN: 1617-4623
    Keywords: GC clusters ; Mobile elements ; Target sites ; mtDNA ; Saccharomyces cerevisiae
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    Topics: Biology
    Notes: Summary GC clusters constitute the major repetitive elements in the mitochondrial (mt) genome of the yeast Saccharomyces cerevisiae. Many of these clusters are optional and thus contribute much to the polymorphism of yeast mtDNAs. We have made a systematic search for polymorphic sites by comparing mtDNA sequences of various yeast strains. Most of the 26 di- or polymorphic sites found differ by the presence or absence of a GC cluster of the majority class, here referred to as the M class, which terminate with an AGGAG motif. Comparison of sequences with and without the GC clusters reveal that elements of the subclasses M1 and M2 are inserted 3′ to a TAG, flanked by A+T rich sequences. M3 elements, in contrast, only occur in tandem arrays of two to four GC clusters; they are consistently inserted 3′ to the AGGAG terminal sequence of a preexisting cluster. The TAG or the terminal AGGAG, therefore, are regarded as being part of the target sites for M1 and M2 or M3 elements, respectively. The dinucleotide AG is in common to both target sites; it also occurs at the 3′ terminus (AGGAG). This suggests its duplication during GC cluster insertion. This notion is supported by the observation that GC clusters of the minor classes G and V similarily repeat at their 3′ terminus a GT or an AA dinucleotide, respectively, from their putative target sites.
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  • 62
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    Molecular genetics and genomics 218 (1989), S. 57-63 
    ISSN: 1617-4623
    Keywords: Saccharomyces cerevisiae ; PET gene ; Transcriptional regulation ; Anaerobiosis ; 5′ mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Translation of mitochondrial cytochrome b RNA in yeast requires the product of the nuclear gene CBS1, a 27.5 kDa soluble mitochondrial protein. In this paper we show that the CBS1 gene is located on chromosome IV immediately adjacent to COX9, the gene coding for cytochrome c oxidase subunit VIIa. CBS1 is transcribed as a very low abundant 900 b RNA. Transcription starts at a single position 101 bp upstream of the CBS1 initiation codon. At positions-39 to-27 of its leader sequence it contains a small open reading frame of 4 codons. By monitoring the β-galactosidase activity of a CBS1/lacZ fusion construct we show that expression of CBS1 is subjected to regulation by oxygen and by glucose: the β-galactosidase activity is elevated threefold in glycerol or galactose grown cells compared to that in glucose grown cells. A further threefold reduction of the activity is observed in anaerobically grown cells. In accordance with this result is the observation that the steady-state level of CBS1 mRNA of anaerobically grown cells is ninefold lower than that of aerobically cultured cells.
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  • 63
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    Euphytica 41 (1989), S. 65-69 
    ISSN: 1573-5060
    Keywords: Fragaria ; Potentilla ; intergeneric hybrids ; pollen storage ; temperature ; relative humidity
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Low temperature and humidity were used for the storage of pollen of four species of Fragaria (2n=14, 42 and 56) and two species of Potentilla (2n=14). The stainability of Fragaria pollen was assessed over a number of years by use of aceto-carmine stain and its viability by cross-pollinations; stainability of Potentilla pollen was assessed by acetocarmine. Fragaria pollen so stored remained stainable for seven years and showed the ability to set seed for three years. over the latter period, aceto-carmine staining gave a reasonable prediction of seed set produced by the stored pollen of Fragaria and so is of value in estimating pollen viability in that genus. Potentilla pollen remained stainable for three years.
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  • 64
    ISSN: 1573-5079
    Keywords: chlorophyll fluorescence ; herbicide binding ; photoinhibition ; photosynthesis ; photosystem II ; temperature
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    Topics: Biology
    Notes: Abstract The time courses of some Photosystem II (PS II) parameters have been monitored during in-vivo and in-vitro photoinhibition of spinach chloroplasts, at room temperature and at 10 °C or 0 °C. Exposing leaf discs of low-light grown spinach at 25 °C to high light led to photoinhibition of chloroplasts in-vivo as manifested by a parallel decrease in the number of functional PS II centres, the variable chlorophyll fluorescence at 77K (F v /F m ), and the number of atrazine-binding sites. When the photoinhibitory treatment was given at 10 °C, the former two parameters declined in parallel but the loss of atrazine-binding sites occurred more slowly and to a lesser extent. During in-vitro photoinhibition of chloroplast thylakoids at 25 °C, the loss of functional PS II centres proceeded slightly more rapidly than the loss of atrazine-binding sites, and this difference in rate was further increased when the thylakoids were photoinhibited at 0 °C. During the recovery phase of leaf discs (up to 9 h) the increases in F v /F m preceded that of the number of functional PS II centres, while only a further decline in the number of atrazine-binding sites was observed. The recovery of variable chlorophyll fluorescence and the concentration of functional PS II centres occurred more rapidly at 25 °C than at 10 °C. These results suggest that the photoinhibition of PS II function is a relatively temperature-independent early photochemical event, whereas the changes in the concentration of herbicide-binding sites appear to be a more complex biochemical process which can occur with a delayed time course.
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  • 65
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    Euphytica 40 (1989), S. 121-126 
    ISSN: 1573-5060
    Keywords: Chrysanthemum morifolium ; chrysanthemum ; tolerance ; temperature ; days to flower ; breeding ; component analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twenty seven clones of chrysanthemum, 19 of which were selected at low temperature, were flowered at 10, 14, 18 and 22°C. The number of days to flower in response to temperature could, for each genotype, be described by a parabola with the parameters: optimum temperature, days to flower at the optimum temperature and curvature which represents the sensitivity to temperature. The low-temperature selections did not have a reduced optimum temperature but reduced sensitivity to temperature accounted for most of the ability to flower at low temperature. The parameters may vary independently. Examples are given to show how these parameters can be combined to construct genotypes with a required temperature response.
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  • 66
    ISSN: 1573-5117
    Keywords: Acanthocyclops ; productivity ; temperature ; food limitation ; generation time
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    Topics: Biology
    Notes: Abstract The generation time of the predatory cyclopoid copepod Acanthocyclops robustus was estimated on 11 occasions during the years 1980 to 1982 in Alderfen Broad. In a multiple regression model, generation time was found to be uncorrelated with temperature, positively correlated (p 〈 0.05) with the densities of Bosmina longirostris and rotifers, and negatively correlated (p 〈 0.001) with the density of nauplii of the calanoid copepod Eudiaptomus gracilis. It is suggested that generation time was determined largely by the availability of calanoid nauplii as prey, even though these constituted only 2% of zooplankton standing biomass.
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  • 67
    ISSN: 1573-5117
    Keywords: Cyclopoid copepods ; development times ; eggs ; instars ; temperature ; diapause
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    Notes: Abstract The duration times of eggs, combined naupliar instars and of the different copepodite stages of five species of cyclopoid copepods — Acanthocyclops robustus, Cyclops vicinus, Diacyclops bicuspidatus, Mesocyclops leuckarti, and Thermocyclops crassus — were investigated at five different temperatures. The five species can be divided in two groups: two species, C. vicinus and D. bicuspidatus, adapted to cold water conditions and three species, A. robustus, M. leuckarti and T. crassus adapted to warm water conditions. The cold water species showed a faster egg development than M. leuckarti and T. crassus at 5–15 °C. The eggs and instars of the warm water species M. leuckarti tend to develop faster than those of the former two species at higher temperatures. A. robustus showed the shortest egg and instar development at 10–25 °C. The warm water species T. crassus produced no eggs at 10 °C and temperatures below. At higher temperatures (20, 25 °C) the egg and instar duration times were similar or longer than those of the other species. When cultured in total darkness a great part of the CIV respectively CV copepodites of the summer forms entered arrest and the percentage of copepodites that showed an arrest of development was highest at lowest temperatures. The present results are compared with data from literature and differences are discussed.
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    Hydrobiologia 186-187 (1989), S. 81-102 
    ISSN: 1573-5117
    Keywords: rotifers ; rate of population increase ; life span ; temperature ; salinity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A review of temperature and salinity effects on rotifer population dynamics is presented together with original data of these effects for three clones of Brachionus plicatilis. There is a clear relationship between temperature and the intrinsic rate of increase, r: an increase of temperature — within the natural environmental range — produces an exponential increase of r, and the slope of the response depends on the genotype. The effect of salinity is also genetically dependent; the highest r for each clone is observed at the salinity close to that of its environmental origin. The response of r to temperature is mainly a consequence of the response of the individual rates of development and reproductive timing. The effect of temperature on fecundity (number of descendents per individual life time) is negligible when temperature values are within the normal habitat ranges. On the other hand, salinity seems to affect primarily fecundity. The interaction salinity-temperature may be important in clones or species living in fluctuating environments with positive response to the more frequent combinations found in the corresponding habitats.
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    Hydrobiologia 186-187 (1989), S. 129-136 
    ISSN: 1573-5117
    Keywords: Hexarthra ; salinity ; alkalinity ; wind action ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Neusiedler See, a shallow alkaline lake with fluctuating water level and salinity, four species of Hexarthra occur: H. mira, H. fennica, H. jenkinae (occasional) and H. polyodonta. The analysis of longterm data reveals a general phenological pattern which does not change from year to year. They first occur in May, develop a maximum in June/July, sometimes a second one in August/September and disappear in October. But the species succession is different in the various years, occasionally only one species (H. mira or H. polyodonta) being present. There is a fairly consistent relation between the chemical conditions and the prevalent species; an increase in salinity favours the development of H. polyodonta. Low temperature and wind generated suspended particles have a negative influence on the development of the Hexarthra populations. Smaller populations of Hexarthra are in a relation to the occurrence of Leptodora indicating predation pressure of the latter species. In Neusiedler See the Hexarthra populations seem to be controlled to a great extent by abiotic factors, but predation by Leptodora and most probably by young fish seems to play an important role too.
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  • 70
    ISSN: 1573-5117
    Keywords: Rotifera ; resting eggs ; light ; temperature ; hatching ; mixis
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    Topics: Biology
    Notes: Abstract The marine rotifer Brachionus plicatilis typicus (Clone 8105A, Univ. of Tokyo) was cultured in 500 ml beakers to form resting eggs. Tetraselmis tetrathele was used as a culture food. Just after formation, resting eggs were exposed to various temperature (5–25 °C) and light regimes (24L: OD and OL : 24D). When eggs were exposed to light just after formation, the eggs hatched sporadically over a month. No hatching was observed for six months when eggs were preserved under dark conditions regardless of the temperature. These eggs hatched simultaneously after being exposed to light and eggs preserved at 5 °C showed twice as high hatching rate (40%) as that of eggs preserved at 15–25 °C (24%). Clones from resting eggs that were kept under different temperature and light regimes were reared individually to the third generation. Incubation at 25 °C with lighting produced the highest (5.4% and 5.2 %) rate of mictic females during their 2nd and 3rd generations, respectively. The lowest rates (0 and 1.5%) were found when the eggs were kept at 5 °C in total darkness for six months. A lower rate of amictic female production was found in clones with higher rates of mixis.
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    Hydrobiologia 186-187 (1989), S. 209-214 
    ISSN: 1573-5117
    Keywords: rotifers ; distribution ; temperature ; oxygen content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lunzer Obersee, a small lake located at an altitude of 1100 m above sea level, was investigated from July 1985 to October 1987. The rotifer community consists of 7 dominant species, 7 subdominant species and 34 species which occasionally occurred in the plankton. The dominant species show rather different demands in relation to temperature and oxygen content; e.g.: Filinia hofmanni was found at a wide range of oxygen concentrations (0.6–13.3 mg O2l−1) and low temperatures (4–6 °O, living in the upper water layers (1–7 m) during spring and in the deeper, anoxic zone in summer. In contrast, Asplanchna priodonta was found at rather high oxygen contents (〉 9 mg O2 l−1), ), and showed a wide range of temperature tolerance (4–15 °C). On the basis of field data the temperature and oxygen requirements of several species are described and discussed.
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    Hydrobiologia 186-187 (1989), S. 363-369 
    ISSN: 1573-5117
    Keywords: rotifers ; temperature ; food concentration ; bioenergetic parameters
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    Topics: Biology
    Notes: Abstract The influence of temperature and food quality was studied on the following energy balance parameters of B. angularis: ingestion, production, growth and mortality. The ingestion rate rises to an optimum at 15 and 20 °C and decreases at 25 °C. The other rates increase continuously over the 5–25 °C range. The Q10-values of production rate are higher than those of ingestion rate. Temperature also modifies the relationship between food concentration and bioenergetic rates. They react according to a Monod function (production at all temperatures, growth at 10 °C) or decrease at high concentrations (growth at 15° and 20 °C.)
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    Hydrobiologia 186-187 (1989), S. 381-386 
    ISSN: 1573-5117
    Keywords: biometric analysis ; resting eggs ; rotifers ; salinity ; temperature ; genetic variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of temperature and salinity on resting egg size of two Brachionus plicatilis (Rotifers) clones was investigated. Clones were selected according to their different behaviour in laying resting eggs: one clone ejects them, whereas they remain inside the females body in the other clone. The difference in resting eggs size between the two clones is noticeable, although the difference is not as great as that between female body size. An important temperature-salinity interaction on resting egg size has been observed. The general inverse relationship between size and temperature is only true at lower temperatures. At high temperatures size varies around the mean although could be greater than at intermediate temperatures. This is more evident at the intermediate salinity tested which is considered to be the closest to the optimum in our experiments. This pattern of variation suggests that mean size is bigger than expected, in relation to temperature and salinity, when these factors have values close to the extremes of their range, normally found in nature, and to which adaptative mechanisms can evolve. Size is bigger at the salinity — temperature low - low and high - high combinations which are the most commonly found in the temperate environments.
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  • 74
    ISSN: 1573-5117
    Keywords: Antarctica ; bacteria ; biofilms ; cyanobacteria ; microbial mats ; Nostoc ; Phormidium ; photosynthesis ; respiration ; stream algae ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Water temperatures in southern Victoria Land streams fluctuated over the range 0 to 10 lay close to freezing. In a series of controlled assays at Fryxell Stream, Taylor Valley, the benthic microbial mats showed strongly positive metabolic responses to increases in temperature well above ambient. Rates of polysaccharide and lipid biosynthesis increased with temperature over the range 0 to 25 °C. Between 0 and 10 °C, Q10 values for the cyanobacterial mats were 1.7 to 3.2 for gross photosynthesis, 2.5 to 5.7 for respiration, 2.2 to 2.5 for acetate incorporation into lipid, 1.9 to 3.8 for glucose catabolism, and 1.9 to 2.8 for thymidine incorporation. Respiration accounted for a high percentage of gross photosynthesis, and a net respiratory loss of carbon from three communities was either induced or worsened by an increase in temperature from 0 to 10 °C. The chlorophyll a content of Nostoc disc incubated for one month in darkness decreased by 27% at 5 °C, but by 99% at 25 °C. This set of assays suggests that the cyanobacterial mats contained large amounts of chlorophyll a and carbon associated with inactive or senescing cells. This unusual standing stock could probably not persist under warmer conditions, which would promote both increased respiratory losses and faster rates of bacterial decomposition.
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  • 75
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    Hydrobiologia 173 (1989), S. 219-229 
    ISSN: 1573-5117
    Keywords: aquatic fungi ; temperature ; seasonal periodicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence, distribution and seasonal periodicity of 49 species of aquatic fungi belonging to the orders Chytridiales, Lagenidiales, Blastocladiales, Saprolegniales and Peronosporales from two tanks of Kurukshetra, India, is described. Seasonal periodicity was found to be governed by temperature.
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  • 76
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    Hydrobiologia 174 (1989), S. 89-97 
    ISSN: 1573-5117
    Keywords: carbon ; Illinois ; light ; nitrate ; nutrient limitation ; periphyton ; phosphate ; streams ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Factors limiting periphyton accrual in east-central Illinois agricultural streams were investigated. Nutrient-diffusing substrata were used to examine periphyton macronutrient limitation in streams in two agricultural watersheds. Substrata consisted of sand-agar mixtures with one of six experimental treatments. Macronutrients included carbon, nitrate, phosphate and combinations of the three. Substrata were collected after a 5 and 9 day period and analyzed for chlorophyll a. None of the treatments were significantly greater than the controls at any of the seven stations, thus we conclude that periphyton in these streams was not nutrient limited. Highest periphyton colonization/growth rates were associated with the smaller upstream reaches, while lower rates occurred in the larger downstream reaches. Multiple regression showed that most of the variance in the rate of chlorophyll a accrual after five days was explained through water temperature and turbidity (r2 = 0.91); whereas, stream nitrate and phosphate concentrations accounted for no significant portion of the variance. We conclude that instream primary production in agricultural streams of central Illinois is limited by temperature and light.
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  • 77
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    Hydrobiologia 179 (1989), S. 17-24 
    ISSN: 1573-5117
    Keywords: profiles ; resolution ; monitoring ; temperature ; oxygen ; spate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Longitudinal profiles of water quality along a well-mixed tidal river are, ideally, based on simultaneous measurements at fixed stations distributed along the river. The resolution of the profiles is limited by the density of the stations. However, for a given number of stations the resolution is greatly increased if water quality date can be extrapolated upstream and downstream of the stations, making use of velocity data; the resolution is then determined by the density of the extrapolated data points, which may be an order of magnitude higher than the density of the stations. A 15-km length of river was investigated using 5 current meters equipped to measure depth, temperature, conductivity and dissolved oxygen. Data were recorded simultaneously every 10 minutes. When the average cross-sectional speed was 0.25 ms−1 (typical of tidal conditions), the extrapolated data points were 150 m apart, so the resolution of the resulting profiles (7 per kilometre) was much higher than that of the stations alone (0.3 per kilometre). The extrapolation process required a means of deducing the average cross-sectional speed from the speed measured at a given station. The key to this was provided by temperature data recorded during the onset of a spate, when tidal flow was suspended and the average cross-sectional speed was uniformly about 0.75 ms −1 at four of the stations. Profiles of temperature and dissolved oxygen were generated by this method; the resolution was about 2 data points km−1 during the onset of the spate, and 6 points km−1 during tidal flow.
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  • 78
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    Hydrobiologia 175 (1989), S. 223-231 
    ISSN: 1573-5117
    Keywords: Rotifera ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Information on the distribution of 225 species of planktic, periphytic and benthic rotifers from diverse waters in south and central Sweden was analyzed for temperature preference and tolerance. Most rotifers have a very wide tolerance range. Certainly differences in temperature dependence exist between separate species. However, these differences are, in a regional material like this, generally less distinct than what has been found for the distribution within individual lakes.
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  • 79
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    Plant and soil 116 (1989), S. 69-76 
    ISSN: 1573-5036
    Keywords: oxidation ; soil ; sulphur ; temperature ; tetrathionate ; thiosulphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The rate of oxidation of micronized elemental sulphur in three soils was measured over a range of temperatures between 2 and 20°C. Temperature had a marked effect with a Q10 (temperature coefficient) between 1.9–3.1. The period for 50% oxidation varied between 6–10 days at 20°C to between 36–42 days at 2°C. All the oxidation curves showed an initial lag. At 20°C the oxidation rate was four times that of flowers of sulphur and was related to the smaller particle size. Additives (wetting and dispersing agents) in the commercial micronized sulphur preparation used (‘Thiovit’) were inhibitory at high concentrations but stimulatory at low concentrations. The significance to field conditions is discussed.
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  • 80
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    Plant cell, tissue and organ culture 18 (1989), S. 167-179 
    ISSN: 1573-5044
    Keywords: Vicia faba L. ; in vitro propagation ; temperature ; nitrogen ; activated charcoal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influences of nitrogen sources, culture temperature and activated charcoal supplements were studied in relation to the rooting ability of V. faba cuttings. The interaction of these factors led to quantitative and qualitative modifications of the culture responses. Low temperatures (14–18°C) were suitable for in vitro culture, limiting the formation of phenolics in plant material and making activated charcoal supplement unnecessary. Nitrogen supplements contributed in modifying the different plant responses, in accordance with temperature. Multiple shoot formation was obtained from the cotyledonary node and from the stem nodes cultivated in the presence of 6-benzylaminopurine (BAP). BAP at 4 mg l-1 was the most effective concentration in promoting high rates of shoot development. The original position of stem nodes was found to determine the explant response to plant growth regulator treatments, possibly due to the effect of residual apical dominance.
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  • 81
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    Journal of bioenergetics and biomembranes 21 (1989), S. 589-603 
    ISSN: 1573-6881
    Keywords: Vacuolar membrane H+ATPase ; vacuoles ; Saccharomyces cerevisiae ; catalytic cooperativity of ATP hydrolysis ; VMA genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Our current work on a vacuolar membrane proton ATPase in the yeastSaccharomyces cerevisiae has revealed that it is a third type of H+-translocating ATPase in the organism. A three-subunit ATPase, which has been purified to near homogeneity from vacuolar membrane vesicles, shares with the native, membrane-bound enzyme common enzymological properties of substrate specificities and inhibitor sensitivities and are clearly distinct from two established types of proton ATPase, the mitochondrial F0F1-type ATP synthase and the plasma membrane E1E2-type H+-ATPase. The vacuolar membrane H+-ATPase is composed of three major subunits, subunita (M r =67 kDa),b (57kDa), andc (20 kDa). Subunita is the catalytic site and subunitc functions as a channel for proton translocation in the enzyme complex. The function of subunitb has not yet been identified. The functional molecular masses of the H+-ATPase under two kinetic conditions have been determined to be 0.9–1.1×105 daltons for single-cycle hydrolysis of ATP and 4.1–5.3×105 daltons for multicycle hydrolysis of ATP, respectively.N,N′-Dicyclohexylcarbodiimide does not inhibit the former reaction but strongly inhibits the latter reaction. The kinetics of single-cycle hydrolysis of ATP indicates the formation of an enzyme-ATP complex and subsequent hydrolysis of the bound ATP to ADP and Pi at a 7-chloro-4-nitrobenzo-2-oxa-1,3-diazolesensitive catalytic site. Cloning of structural genes for the three subunits of the H+-ATPase (VMA1, VMA2, andVMA3) and their nucleotide sequence determination have been accomplished, which provide greater advantages for molecular biological studies on the structure-function relationship and biogenesis of the enzyme complex. Bioenergetic aspects of the vacuole as a main, acidic compartment ensuring ionic homeostasis in the cytosol have been described.
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  • 82
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    Journal of bioenergetics and biomembranes 21 (1989), S. 115-135 
    ISSN: 1573-6881
    Keywords: Membrane fluidity ; fish membranes ; homeoviscous adaptation ; temperature ; hydrostatic pressure ; acclimation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The homoeostatic regulation of bilayer order is a property of functional importance. Arguably, it is best studied in those organisms which experience and must overcome disturbances in bilayer order which may be imposed by variations in temperature of hydrostatic pressure. This article reviews our recent work on the adaptations of order in brain membranes of those fish which acclimate to seasonal changes in temperature or which have evolved in extreme thermal or abyssal habitats. The effects of temperature and pressure upon hydrocarbon order and phase state are reviewed to indicate the magnitude of the disturbances experienced by animals in their environments over the seasonal or evolutionary timescale. Acclimation of fish to altered temperature leads to a partial correction of order, while comparison of fish from extreme cold environments with those from temperate or tropical waters reveals a more complete adaptation. Fish from the deep sea also display adaptations of bilayer order which largely overcome the ordering effects of pressure.
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  • 83
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    Journal of bioenergetics and biomembranes 21 (1989), S. 621-632 
    ISSN: 1573-6881
    Keywords: ATPase ; [H+]-ATPase ; proton transport ; Neurospora crassa ; Saccharomyces cerevisiae ; Schizosaccharomyces pombe
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    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract The fungal plasma membrane contains a proton-translocating ATPase that is closely related, both structurally and functionally, to the [Na+, K+]-, [H+, K+]-, and [Ca2+]-ATPases of animal cells, the plasma-membrane [H+]-ATPase of higher plants, and several bacterial cation-transporting ATPases. This review summarizes currently available information on the molecular genetics, protein structure, and reaction cycle of the fungal enzyme. Recent efforts to dissect structure-function relationships are also discussed.
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  • 84
    ISSN: 1573-5087
    Keywords: Abscisic acid ; dormancy ; batatasin-I ; phthalic acid ; temperature ; Dioscorea floribunda
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the tubers of medicinal yam (Dioscorea floribunda Mart. & Gal.) abscisic acid (ABA) content was high in all the parts during the dormant condition in the winter, but it decreased to a very low level in the actively growing plants. ABA content of the entire tuber was negatively correlated with temperature and photoperiod. Growth inhibitors including batatasin-I and phthalic acid were identified in the dormant tuber.
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  • 85
    ISSN: 1573-904X
    Keywords: ultrasound ; phonophoresis ; temperature ; ibuprofen ; human skin ; in vitro percutaneous absorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The objective of this study was to develop an in vitro method to investigate the effect of ultrasound on the in vitro absorption of ibuprofen from a propylene glycol/water vehicle through human epidermis. A diffusion cell was modified so ultrasound could be applied to the vehicle and skin. Since ultrasound can increase the temperature underneath the area of application, control representing temperature effects ran concurrently to the ultrasound experiment. The results demonstrate that ultrasound can increase the penetration of ibuprofen through human skin. This increase in diffusion was greater than for controls where an equivalent increase in temperature was utilized. The results also indicate that evaporation of vehicle components may alter the skin/vehicle partition coefficient, decreasing the effects of ultrasound on the penetration of ibuprofen through the skin.
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  • 86
    ISSN: 1573-8248
    Keywords: Telenomus reynoldsi ; functional response ; Holling disc equation ; random parasite equation ; temperature ; Telenomus reynoldsi ; réponse fonctionnelle ; équation de Holling ; équation de la recherche au hasard de l'hôte ; température
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé La réponse fonctionnelle deTelenomus reynoldsi Gordh et Coker à l'augmentation de la densité des œufs deGeocoris punctipes (Say) a été déterminée à 5 températures constantes en utilisant des boîtes de Pétri. Les courbes obtenues à toutes les températures étaient de Type II. Les durées du parasitisme (Th) et les taux de recherche de l'hôte (a) ont été estimés et comparés en utilisant l'équation «disc» de Holling. Le taux maximum du parasitisme (1/Th) s'est accru linéairement de 9 à 15 hôtes/jour avec l'accroissement de la température. La meilleure représentation de la relation entre le taux de recherche de l'hôte et la température a été obtenue par une équation quadratique. Le taux de recherche de l'hôte variait de 205 à 888 cm2/jour; il était maximum à 32° C. En utilisant l'équation du «random parasite» (parasite recherchant son hôte au hasard), l'analyse de la réponse fonctionnelle dans un terrain plus complexe contenant une plante artificielle, a conduit à des estimations plus précises du taux de recherche de l'hôte et du taux maximum du parasitisme, qui peuvent être utilisées pour décrire ces activités dans la nature. Le taux de la recherche de l'hôte était de 6680 cm2/jour, tandis que le taux maximum du parasitisme était de 13 hôtes/jour.
    Notes: Abstract The functional response ofTelenomus reynoldsi Gordh & Coker was determined at 5 constant temperatures using a dish arena. Functional response curves at all temperatures were of Holling's Type II. Handling rate increased linearly with increasing temperature, whereas the relationship of search rate to temperature was best described by a quadratic model. Search rate was highest at 32°C. Using Rogers' random parasite equation, analysis of the functional response in a more complex arena containing an artificial plant provided estimates of search and handling rates that may be more appropriate in the field environment. Search rate was 6,680 cm2/day. Handling rate was 13 hosts/day.
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  • 87
    ISSN: 1573-8248
    Keywords: Notonecta ; embryos ; temperature ; Notonecta ; embryon ; température ; Notonecta ; embriones ; temperatura
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Les œufs deNotonecta unifasciata Guérin à différents stades de développment embryonnaire maintenus dans l'eau à des températures variables (2,2–25 °C) et durant de 4 à 12 semaines révèlent une viabilité maximale (〉80 %) à la température la plus forte. Cependant, la viabilité optimale sans développement se situait à 14.4 °C avec des embryons âgés de 8 jours (〉35 %). Un court stockage (4 semaines) à 14.4 °C augmente d'une façon significative la viabilité de l'œuf. La survie était faible (〈20 %) aux 2 températures les plus basses. Des œufs maintenus à 14.4 °C durant 12 semaines et supportant environ 50 % de mortalité peuvent être un procédé pratique de lutte biologique.
    Abstract: Resúmen Huevecillos deNotonecta unifasciata mantenidos en varios estadios del desarrollo embrionarios y a varias temperaturas (2.2–25.6 °C) por 4–12 semanas, demostraron la mayor viabilidad (〉80 %) a la temperatura más alta. Sín embargo, la óptima viabilidad cuando no hubo desarrollo, se realizó en embriones de ocho días de edad, mantenidos a 14.4 °C. Almacenamiento de corto tiempo (cuatro semanas) a 14.4 °C se produjo un significante aumento de viabilidad. Superviviencia fué baja (〈20 %) a temperaturas menos de 8.3 °C. Se ofrece que huevecillos almacenados a 14.4 °C por 12 semanas, cuales se realizan un 50 % mortalidad, puede servir en la ejecución del control biológico.
    Notes: Abstract Notonecta unifasciata Guerin eggs maintained at different stages of embryonic development in water at variable temperatures (2.2–25.6 °C) and for periods of 4–12 weeks revealed maximum viability (〉80 %) at the highest temperature. However, optimum nondevelopmental viability was at 14.4 °C with eight-day-old embryos (〉35 %). Short term (4 weeks) storage at 14.4 °C significantly increased egg viability. Survival was poor (〈20 %) at the 2 lowest temperatures. Eggs held at 14.4 °C for 12 weeks and sustainingca. 50 % mortality, may be a practical procedure for biological control.
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  • 88
    ISSN: 1573-8248
    Keywords: Anagyrus pseudococci ; Leptomastix dactylopii ; Leptomastidea abnormis ; temperature ; adult longevity ; progeny production ; Anagyrus pseudococci ; Leptomastix dactylopii ; Leptomastidea abnormis ; température ; longévité des adultes ; descendance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé La descendance augmente et la longévité de l'adulte décroît quand la température augmente entre 18°C et 30°C pour les 3 parasitoïdes de pseudococcines,Anagyrus pseudococci, (Girault),Leptomastix dactylopii Howard etLeptomastidea abnormis Girault. La distribution de Weibull donnait une bonne adaptation aux courbes de suvies pour les 3 parasitoïdes et la comparaison statistique à différentes températures des paramètresb etc de Weibull permettaient des changements dans l'échelle et la forme des courbes. En général les ♀♀ des 3 espèces vivaient plus longtemps que les ♂♂, excepté aux fortes températures. Les femelles deL. abnormis avaient leur descendance maximale à 24°C et la maintenaient à ce niveau jusqu'à 34°C. Elles vivaient plus longtemps que les 2 autres espèces de parasitoïdes à 30°C et manifestaient une courbe de survie de type I dans la gamme de températures examinées.A. pseudococci etL. dactylopii réclamaient tous deux de fortes températures (30°C) pour atteindre leur descendance maximale, mais vivaient moins longtemps à cette température. A 30°C les importante de la population qui mourrait dans les tous premiers jours.L. dactylopii vivait le plus longtemps à 26°C, les femelles manifestant une courbe de survie de type I à toutes les températures, tandis que les courbes de survie des mâles passaient du type I au type II à 30°C. Les implications de ces observations dans la dynamique des populations des différents parasitoïdes sont discutées brièvement.
    Notes: Abstract Progeny production increased and adult longevity decreased with rising temperature within the range 18°C to 30°C for the 3 mealybug parasitoidsAnagyrus pseudococci (Girault),Leptomastix dactylopii Howard andLeptomastidea abnormis (Girault). The Weibull distribution gave a good fit to survival curves for the 3 parasitoids and statistical comparison of Weibullb andc parameters at different temperatures allowed changes in the scale and shape of the curves to be detected. In general, ♀♀ lived longer than ♂♂ for all 3 species, except at high temperature. FemaleL. abnormis attained their maximum progeny production at 24°C and maintained this level up to 34°C. They lived longer than the other 2 parasitoid species at 30°C and showed a type I survival curve throuhout the range of temperatures examined.A. pseudococci andL. dactylopii both required high temperatures (30°C) to attain their maximal progeny production, but werepseudococci tended towards type II, with a larger proportion of the population dying within the first few days.L. dactylopii lived longest at 26°C, with ♀♀ showing a type I survival curve at all temperatures and ♂ survival curves changing from type I to type II at 30°C. The implications of these findings for the population dynamics of the different parasitoids are briefly discussed.
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    Entomologia experimentalis et applicata 48 (1988), S. 73-84 
    ISSN: 1570-7458
    Keywords: Liriomyza trifolii ; Agromyzidae ; Diptera ; temperature ; thresholds ; life history ; feeding ; intrinsic rate of increase ; tomato ; host-plant suitability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé La vitesse de développement, la mortalité, l'alimentation, la fécondité et la longévité de L. trifolii (Burgess) élevés sur plants de tomates du cultivar ‘Moneydor’ ont été examinées au laboratoire sous 3 températures constantes (15°C, 20°C et 25°C) et une thermopériode (16/22°C, moyenne 19,5°C). Les taux de développement et les seuils de chaque stade ont été déterminés à partir des droites de régressions. Aucune corrélation n'a été mise en évidence entre ces variables biologiques et la taille des pupes. De plus, certaines données sur la biologie de L. trifolii sont fournies et discutées. Le taux intrinsèque d'accroissement, rm a varié de-0,0023 oeuf viable/femelle/jour à 15°C à 0,1254 oeuf viable/femelle/jour à 25°C et la reproduction nette de 1 oeuf femelle viable/femelle à 15°C à 26 oeufs femelles viables/femelle à 25°C. La durée d'une génération a varié de 48 jours à 15°C à 24 j à 25°C. 90% de la ponte a eu lieu dans les 115 premiers degrés jours de la vie imaginale à 20 et 25°C. La fécondité et la longévité étaient fortement liées au nombre de piqûres nutritionnelles. Ces résultats montrent que la tomate est un hôte convenable permettant aux populations de L. trifolii de se développer lorsque la température est supérieure à 16°C.
    Notes: Abstract The effects of three constant (15°C, 20°C and 25°C) and one alternating (16–22°C, mean 19.5°C) temperatures on development, mortality, feeding, fecundity and longevity of Liriomyza trifolii (Burgess) on tomato plants cv. ‘Moneydor’ were examined in the laboratory. Development rates and thresholds for each instar were estimated by means of linear regression. No correlation was found between life history variables and pupal length. Further, data on the biology of L. trifolii are given and discussed. The intrinsic rate of increase, rm, varied from-0.0023 viable female eggs/⧫/day at 15°C to 0.1254 eggs/⧫/day at 25°C and net reproduction from one viable female egg/⧫ at 15°C to 26 eggs/⧫ at 20°C. Generation time varied from 48 days at 15°C to 24 days at 25°C. Ninety % oviposition occurred within the first 115 degree-days of adult life at both 20°C and 25°C. Fecundity and longevity were highly correlated with the number of feeding punctures. The data indicate that tomato is a suitable host plant allowing populations of L. trifolii to increase if temperatures are above 16°C.
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  • 90
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Encyrtidae ; parasitoids ; Pseudococcidae ; mealybugs ; Anagyrus pseudococci ; Leptomastix dactylopii ; Leptomastidea abnormis ; temperature ; predicting development ; thresholds ; temperate glasshouses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les vitesses de développement de 3 parasitoïdes de cochenilles ont été étudiées dans les conditions de température de serre, avec thermopériode ou en température constante. En conditions constantes, la vitesse augmente jusqu'à 35°C, mais est létale à 40°C. Avec thermopériode, une thermophase de 40°C peut être tolérée pendant 12 heures. Les vitesses de développement sous thermopériodes pourraient être calculées à partir des données obtenues en conditions constantes, modifiées en fonction des durées respectives des cryophases et thermophases. Une méthode originale est présentée pour obtenir les seuils inférieurs de développement, en utilisant des thermopériodes, et pour calculer les vitesses de développement à températures élevées. Une méthode de régression polynomiale, non vérifiée préalablement, a été utilisée pour prévoir les durées moyennes des développements sous serre. Ces estimations sont beaucoup plus sûres que les prédictions basées sur le concept de degré-jour (Do), lorsque les températures extrêmes durent longtemps, mais en serre chaude elles ne présentent aucune différence significative. De plus, une méthode d'évaluation des 95% d'émergences, a donné de bonnes estimations des premières et dermières émergences en conditions périodiques.
    Notes: Abstract Developmental rates of three encyrtid mealybug parasitoids were examined under constant, cycling and glasshouse temperatures. Under constant conditions the rate of development increased with rising temperature up to 35°C, but a constant 40°C was lethal. Under cycling conditions, 12 h periods at 40°C could be tolerated. Developmental rates under cycling conditions could be calculated from constant temperature data, by incrementing development on an h/h basis. A new technique was developed to determine the lower thresholds for development, using cycling regimes and to calculate the rates of development over short periods at high temperature. A previously untested polynomial regression technique was used to predict mean developmental periods under glasshouse conditions. These predictions were considerably more accurate than day-degree (Do) predictions when substantial periods at extreme temperatures were involved, but not significantly different from day-degree predictions under ‘hot-house’ conditions. In addition a method was developed to calculate a 95% range for emergence, which gave consistently good estimates of first and last eclosion observed under fluctuating conditions.
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    Entomologia experimentalis et applicata 47 (1988), S. 173-182 
    ISSN: 1570-7458
    Keywords: Bembidion lampros ; Pterostichus cupreus ; Carabidae ; Coleoptera ; behaviour ; temperature ; searching ; consumption ; cereal aphid ; Rhopalosiphum padi ; prey ; density ; barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les taux maximum de consommation de R. padi L. à différentes températures ont été déterminés au laboratoire chez deux carabes, B. lampros Herbstet P. cupreus L. La consommation moyenne a augmenté avec la température chez les deux espèces, B. lampros consommant un maximum de 15,9 larves des stades 1 à 3 et 9,1 pucerons adultes aptères, à 25°C. P. cupreus a été particulièrement vorace et a consommé 125,3 adultes aptères par jour à 20°C. Le comportement des deux espèces a été observé en filmant en vidéo des carabes à jeun, maintenus à différentes températures constantes, dans des enceintes semées en orge de printemps. Des éléments du comportement, communs aux deux espèces, ont été définis: 1) immabilité, 2) marche et course, 3) recherche, 4) affrontement. P. cupreus a été plus actif à toutes les températures, B. lamprosa été inactif au-dessous de 10°C. La part de temps consacrée à la recherche, le nombre de plantes prospectées, et la vitesse ont augmenté avec la température chez les deux espèces. Dans des enceintes similaires colonisées par R. padi, P. cupreus a significativement augmenté le temps consacré à la recherche dans les enceintes, parallèlement à l'augmentation de la densité des pucerons. Après la découverte d'une colonie de pucerons, P. cupreus escalade et prospecte la plante et ses voisines immédiates; tandis que les plantes des enceintes sans pucerons sont rarement escaladées. B. lampros n'a pas été observé escaladant des plantes d'enceintes avec ou sans pucerons, et il n'a pas accru son temps de prospection en fonction de la densité de pucerons. Les quelques B. lampros qui ont capturé des pucerons l'ont fait lorsque ceux-ci marchaient sur la surface du sol. La discussion a porté sur l'efficacité relative des deux carabes comme prédateurs de R. padi, et les résultats ont été comparés à ceux d'études du même type, menées ailleurs, avec des prédateurs de Sitobion avenae sur blé d'hiver.
    Notes: Abstract Maximum consumption rates were determined for two carabids, Bembidion lampros Herbst. and Pterostichus cupreus L., feeding on the cereal aphid Rhopalosiphum padi L. at different temperatures in the laboratory. Mean daily consumption increased with increasing temperature for both species, B. lampros consuming a maximum of 16 1–3 instar nymphs and 9 apterous adult aphids at 25°C. P. cupreus was particularly voracious and consumed 125 apterous adult R. padi per day at 20°C. The behaviour of both species was analysed by video filming starved beetles, maintained at different constant temperatures, in arenas sown with spring barley. The behavioural components (1) still; (2) run/walk; (3) search and (4) confrontation were identified and were common to both species. P. cupreus was more active over the temperature range tested; B. lampros was inactive under 10°C. The proportion of time spent searching, number of plants searched, and velocity increased with increasing temperature for both species. When observed in similar arenas seeded with R. padi colonies, individuals of P. cupreus significantly increased their time spent searching in arenas with increasing aphid density. Following discovery of an aphid colony, individuals climbed and searched the host plant and its nearest neighbours. Plants in aphid free arenas were rarely climbed. B. lampros was not observed climbing in either aphid free arenas or in arenas with increasing aphid densities, and did not significantly increase its time spent searching in response to increased prey density. The few B. lampros that found aphids caught them walking on the soil surface. The relative efficiences of these two carabids as predators of R. padi are discussed, and the results are compared with similar studies elsewhere with predators of Sitobion avenae on winter wheat.
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  • 92
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Site specific recombination ; 2 μ DNA plasmid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The purpose of this work is to identify and quantitate in vivo 2 μ plasmid FLP-independent recombination in yeast, using a nonselective assay system for rapid detection of phenotypic expression of the recombination events. A tester plasmid was constructed such that in vivo recombination between 2 μ direct repeat sequences produces the resolution of the plasmid into two circular DNA molecules. This recombinational event is detected as a phenotypic shift from red to white colonies, due to the mitotic loss of the plasmid portion containing the yeast ADE8 gene in a recipient ade1 ade2 ade8 genetic background. In the absence of the 2 μ FLP recombinase and/or its target DNA sequence, recombination is not abolished but rather continues at a high frequency of about 17%. This suggests that the FLP-independent events are mediated by the chromosomally-encoded general homologous recombination system. We therefore conclude that the totality of 2 μ DNA recombination events occurring in FLP+ cells is the contribution of both FLP-mediated and FLP-independent events.
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  • 93
    ISSN: 1432-0983
    Keywords: Schizosaccharomyces pombe ; Saccharomyces cerevisiae ; Argininosuccinate lyase ; Gene cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A gene bank of Sau3A partially restricted Schizosaccharomyces pombe DNA in YEp13 was used to transform an arg4 mutant of Saccharomyces cerevisiae. One colony was recovered which contained the YEp13 plasmid bearing a large insert complementing the argininosuccinate lyase (ASL) mutation. As shown by restriction mapping and subcloning experiments, the DNA sequence required for complementation is localized on a 2 kb BamHI-BamHI fragment. The plasmid complemented several S. cerevisiae arg4 mutants of independent origin and a S. pombe arg7 mutant lacking ASL. Low but significant ASL activities were detected in crude extracts of these transformants. No complementation of the E. coli argH mutant was observed. Southern blot hybridizations showed that the insert originates from the S. pombe genome. No cross-hybridization was found between this sequence and S. cerevisiae DNA. It can be concluded that the cloned DNA fragment bears the S. pombe ARG7 gene coding for ASL.
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  • 94
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    Current genetics 14 (1988), S. 413-418 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Chromosome length polymorphisms ; FIGE ; OFAGE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Although Saccharomyces cerevisiae strains generally have similar chromosomal band patterns as revealed by pulsed field gel electrophoresis, individual bands often move slightly differently from one strain to the other. Surveying strains from our stock collection, we found that nearly all the bands of a certain pair of strains differed in their mobility. Some of these chromosome length polymorphisms segregated in a 2:2 ratio, indicating that they resulted from single structural alterations (i.e. additions or deletions). One of these was mapped on the right arm of chromosome 1. Others did not segrate in a simple 2:2 ratio. That is, there were progenies which had bands not present in either parent. We suggest that these new bands are the products of recombination between homologous chromosomes having two or more structural alterations.
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  • 95
    ISSN: 1432-0983
    Keywords: ARS ; Saccharomyces cerevisiae ; Schizosaccharomyces pombe ; Tetrahymena thermophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have isolated several Tetrahymena thermophila chromosomal DNA fragments which function as autonomously replicating sequences (ARS) in the heterologous Saccharomyces cerevisiae and Schizosaccharomyces pombe selection systems. The Tetrahymena ARS sequences were first isolated in S. cerevisiae and were derived from non-ribosomal micro- and macronuclear DNA. Sequence analysis of the ARS elements identified either perfect or close matches with the 11 by S. cerevisiae ARS core consensus sequence. Subcloning studies of two Tetrahymena ARS elements defined functional regions ranging in size from 50 to 300 bp. Testing of the ARS elements in S. pombe revealed that most of the T. thermophila inserts confer ARS function in both yeasts, at least in the sense of promoting a high transformation frequency to plasmids which contain them. However, the actual sequences responsible for ARS activity were not always identical in the two yeasts.
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  • 96
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Ribosomal protein genes ; CYH2
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A diploid strain of the yeast Saccharomyces cerevisiae has been constructed that has one copy of the ribosomal protein gene CYH2 completely deleted and replaced with the TRP1 gene using the method of Rothstein (1983). There are only small differences in growth rate and no detectable difference in steady state level of CYH2 mRNA between the diploid that is heterozygous for the CYH2 deletion and the parent diploid with two normal copies of this gene. This suggests that the diploid must partially compensate for the loss of one CYH2 gene. Tetrad dissection shows that haploid spores lacking the CYH2 gene cannot germinate. The lethality of this deletion can be rescued by a CYH2 cDNA on a low copy vector. Haploids which lack the genomic copy of the CYH2 gene, but contain a plasmid copy of the CYH2 cDNA are able to grow normally. These CYH2 deleted yeast haploids should be useful to analyze mutationally altered CYH2 genes and genes homologous to CYH2 from other organisms without interference from a genomic copy.
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  • 97
    ISSN: 1432-0983
    Keywords: Yeast ; Gene regulation ; Saccharomyces cerevisiae ; PDCI promoter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 870 by promoter fragment of the PDC1 gene that includes the carbon source dependent regulatory regions was investigated using 5′ and 3′ promoter deletions. The results indicate that glucose and ethanol regulation of PDC1 transcription are independently controlled by distinct cis-acting regions. The consensus sequence AAATCGATA may play a role in this regulation, while the sequence (ATCA)AACCT may be important in transcription initiation.
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  • 98
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    Current genetics 13 (1988), S. 21-23 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Transformation ; Plasmid ; Colony ; Polyethylene glycol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A rapid and simple yeast transformation procedure has been developed using colonies on agar plates. Saccharomyces cerevisiae SHY3 cells were picked up from colonies on YPD plates grown freshly or stored at 4 °C and incubated with M13RK9-T DNA at 30 °C for 1–2 h in a solution of Li+, Ca2+, Mg2+, triacetin and polyethylene glycol. About 3,500 transformants were obtained per µg of double stranded M13RK9-T DNA. Unlike the existing spheroplast techniques, single stranded M13RK9-T DNA transformed intact cells below one-hundredth frequency of the duplex form.
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  • 99
    ISSN: 1432-0983
    Keywords: Aspergillus terreus clonotheque ; Saccharomyces cerevisiae ; Homologous integration ; 2 μ circledirected chromosome destabilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A genome clonotheque consisting of 25- to 40-kb Sau3A1 fragments of Aspergillus terreus DNA was constructed in the episomal cosmid vector pES33 containing the yeastARG4 gene. From the 475 transformants of cir° yeast strain ESH-0, 23 stable Arg + transformants were independently selected. Genetic and Southern analysis of these stable transformants showed that 39% arose as a result of recombination between cloned A. terreus DNA sequences and yeast chromosome XII. The recombination events most likely occurred in the regions of homology within the rDNA clusters of A. terreus and Saccharomyces cerevisiae.
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  • 100
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    Current genetics 13 (1988), S. 283-289 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Inducible antisense gene ; Acetolactate synthase ; Bradytrophic phenocopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We report for the first time on the regulation of gene expression in yeast by antisense RNA. Chimaeric genes were constructed containing the 5′ upstream and partial coding sequence of SMR1 — a sulfometuron methyl resistant allele of the ILV2 locus. Such fragments were placed 5′ to 3′ and 3′ to 5′ under control of the GAL10 promoter and CYCl terminator in a high copy YEp plasmid. Following galactose induction only transformants containing antisense RNA genes showed biological activity against SMR1 gene expression. Antisense RNA inhibited synthesis of the SMR1 gene product acetolactate synthase and thus repressed cellular growth which resulted in a bradytrophic auxotroph revertable by addition of isoleucine and valine. Antisense RNA inhibition was enhanced in galactose medium containing sulfometuron methyl and in gcn4 cells deficient for positive regulation of the ILV2 locus. This system can be used to study factors that interfere with antisense RNA function and to assign biological function to randomly cloned DNA fragments.
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