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  • taxonomy  (67)
  • Triticum aestivum
  • Springer  (115)
  • American Meteorological Society
  • 2005-2009
  • 2000-2004  (115)
  • 1925-1929
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  • Springer  (115)
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  • 1
    ISSN: 1439-0361
    Keywords: Biogeography ; geographic variation ; taxonomy ; Amazilia ; Trochilidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Die vorliegende Studie stellt die Ergebnisse von Untersuchungen zur Biogeographie, geographischen Variation, Habitatwahl und Taxonomie der Lesson-Amazilie (Amazilia amazilia) vor. Das Taxon umfaßt gegenwärtig fünf Unterarten, die von W-Ecuador bis SW-Peru verbreitet sind und überwiegend submontane Lebensräume besiedeln. Die Ventralfärbung unterliegt klinaler Variation; weiße Gefiederanteile nehmen von nördlichen zu südlichen Populationen ab, rotbraune Partien dagegen zu. Eigenständige morphologisch-morphometrische Kennzeichen sowie Unterschiede in den Habitatpräferenzen und in der Gesangsstruktur rechtfertigen es, die bisherige UnterartA. a. alticola als Paraspezies (A. alticola, Loja-Amazilie) anzuerkennen. Der Vulgärname weist auf den rezenten Verbreitungsschwerpunkt in den südecuadorianischen Anden hin. Aufgrund der engen Verwandtschaft bildenA. amazilia undA. alticola sowie die in Kolumbien endemische ArtA. castaneiventris eine Superspezies.
    Notes: Summary This paper reviews the biogeography, geographic variation, and habitat characteristics of the Amazilia Hummingbird (Amazilia amazilia). Based on morphological features, habitat preferences, and bioacoustics, I propose that the currently recognized subspeciesA. a. alticola should be regarded as a species distinct fromA. amazilia. Synapomorphic characters found in both taxa indicate membership of the same superspecies, together with the Colombian speciesA. castaneiventris. Considering the distributional focus ofA. alticola in the Andes of southern Ecuador, the English species name Loja Hummingbird is suggested.
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  • 2
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    Journal of ornithology 141 (2000), S. 275-284 
    ISSN: 1439-0361
    Keywords: East Asia ; taxonomy ; species delimitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Über die Gliederung der ostasiatischen Dickschnabelkrähen (Corvus macrorhynchos s.l.) in Biospezies besteht bisher keine einhellige Meinung. In dem großen Verbreitungsgebiet zwischen Indien, Japan und Timor werden gegenwärtig eine, zwei oder drei Arten anerkannt. Hier versuchen wir, in akustischen Merkmalen artanzeigende Merkmale zu finden. Eine Diskriminanzanalyse der Rufstruktur (76 Parameter) trennt dreiCorvus-Taxa (levaillantii undjaponensis aus der ‘macrorhynchos-Gruppe’,splendens) von der Südabdachung des Himalaya (Nepal, Kashmir) vollständig voneinander. BeiC. japonensis bleiben die trennenden Merkmale über große geographische Gebiete konstant. Die akustischen Unterschiede werden als taxonomisch relevant angesehen; sie zeigen Artgrenzen an. Folglich werden die Dickschnabelkrähen aus den Tief- und Hochlagen Nepals (Kontaktzone um 2 000 m) zwei Biospezies zugeordnet (C. japonensis undC. levaillantii), in Übereinstimmung mit morphologischen, ökologischen und parasitologischen Daten. Taxonomische Extrapolation der Befunde auf die Systematik anderer ‘macrorhynchos’-Populationen erscheint über die Rufstruktur (bis zum Fernen Osten Rußlands) und über Federling-Parasiten (Mallophaga) auf große Teile SO-Asiens möglich, bedarf aber für die stimmlichen Merkmale noch weiterer Klärung. Wahrscheinlich müssen immacrorhynchos-Komplex mindestens drei biologische Arten anerkannt werden (C. japonensis, C. levaillantii, C. macrorhynchos s. str.).
    Notes: Summary A discriminant analysis of the call structure completely separates threeCorvus taxa (levaillantii andjaponensis of the ‘macrorhynchos group’,splendens) inhabiting the southern slopes of the Himalayas (Nepal, Kashmir). The acoustic differences are considered taxonomically relevant, indicating species boundaries. Therefore the Jungle Crows of lower and upper Nepal (contact zone at around 2,000 m) are assigned to two biospecies (C. japonensis andC. levaillantii), as is consistent with morphological, ecological and parasitological data. It appears possible to extrapolate the findings regarding call structure to the systematics of other ‘macrorhynchos’ populations as distant as the Russian Far East, and those regarding mallophagan parasites to large areas of SE Asia; regarding the vocal characteristics, however, further clarification is needed.
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  • 3
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    Conservation genetics 1 (2000), S. 67-76 
    ISSN: 1572-9737
    Keywords: mitochondrial DNA ; phylogeny ; phylogeography ; speciation ; species concepts ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 4
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    Nutrient cycling in agroecosystems 56 (2000), S. 59-68 
    ISSN: 1573-0867
    Keywords: bicarbonate-extractable potassium ; muriate of potash ; potassium ; potassium chloride ; relative effectiveness ; silicate rock powder ; Triticum aestivum ; Trifolium subterraneum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Granite (silicate) rock dust, a by-product of quarry operations, is being advocated and used as a fertilizer in the wheatbelt of south-western Australia (WA). The dust is insoluble and based on its nutrient element content (1.9% K and 0.3%P and negligible N) it is not expected to be a useful fertilizer. Previous laboratory studies and glasshouse experiments in WA suggest the dust is a slow release K fertilizer. This paper extends the previous studies to consider the dust as an NP or K fertilizer in the year of application in a field experiment on a soil deficient in N, P and K. In addition, the effectiveness of the dust as a K fertilizer was compared with the effectiveness of KCl (muriate of potash), the K fertilizer used in WA at present, in glasshouse experiments using K deficient soils. In the field experiment, compared with NP fertilizer or NPK fertilizer (urea, supplying N; superphosphate, providing P, S, Ca, Cu, Zn and Mo; KCl providing K), the dust had no effect on grain yield of wheat (Triticum aestivum); in fact dust applied at 20 t ha-1, for unknown reasons, reduced yields by about 65% compared to the nil (no fertilizer, no dust) treatment. Relative to the nil treatment, applying NPK fertilizer increased yields about threefold, from 0.54 to 1.79 t ha. The glasshouse experiments showed that, relative to KCl, the dust was from about 0.02 to 14% as effective in K deficient grey sandy soils for producing dried tops of 30-day old wheat plants or 42-day old clover (Trifolium subterraneum) plants. In soils with adequate K (yellow sands, sandy loams or clays, loamy clays, clay loams and clays), neither KCl nor the dust affected yields of 30 to 42-day old wheat or clover plants grown in the glasshouse. In the glasshouse experiments, no yield depressions were measured for the dust applied up to 17 g dust per kg soil (equivalent to 17 t dust ha-1 mixed into the top 10 cm of soil in the field). It is concluded that the dust has no value as a fertilizer.
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  • 5
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    Nutrient cycling in agroecosystems 56 (2000), S. 117-123 
    ISSN: 1573-0867
    Keywords: labelled nitrogen ; Lolium perenne ; nitrogen cycling ; root biomass ; straw ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Large amounts of nitrogen (N) fertiliser (150–200 kg N/ha) are currently being applied to perennial ryegrass (Lolium perenneL.) seed crops in New Zealand. Due to increasing requirements for efficient use of N fertilisers and minimising nitrate contamination of the environment, a field experiment was established using 15N-labelled fertiliser to follow the fate of applied N. Urea-15N was applied to a perennial ryegrass seed crop in April (30 kg N/ha), August (30 kg N/ha), September (60 kg N/ha) and October (60 kg N/ha). The urea-15N was applied in solution and watered in to minimise volatilisation loss. At the time of harvest (December), 9% of the applied 15N was in the seed, 29% in the straw, 19% in the roots and 39% in the soil organic matter. Losses of 15N were minimal as the N was applied in several applications, each one at a relatively low rate, and at times when leaching was unlikely to occur. Ryegrass plants used a greater proportion of the N applied in September and October (61–65%) compared with that applied in April (44%). Consequently more N was recovered from the soil in the autumn application (57%) than from the September and October applications (28–44%). The availability of the residual fertiliser N to a subsequent wheat (Triticum aestivum L.) crop was studied in a glasshouse experiment. The residual fertiliser N was present in the soil and ryegrass roots and stubble. The wheat plants only recovered 7–9% of this residual N. Most of the N taken up by the wheat came from the soil organic N pool. Overall, applying a total of 180 kg N/ha to the ryegrass appeared to have minimal direct impact on the environment. In the short term N not used by the ryegrass plants contributed to the soil organic N pool.
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  • 6
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    Journal of the history of biology 33 (2000), S. 27-70 
    ISSN: 1573-0387
    Keywords: ants ; E. O. Wilson ; Ernst Mayr ; systematics ; Systematics and the Origin of Species ; taxonomy ; William L. Brown ; William Morton Wheeler ; William Steel Creighton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , History
    Notes: Abstract Ideas about the natural world are intertwined with the personalities, practices, and the workplaces of scientists. The relationships between these categories are explored in the life of the taxonomist William Steel Creighton. Creighton studied taxonomy under William Morton Wheeler at Harvard University. He took the rules he learned from Wheeler out of the museum and into the field. In testing the rules against a new situation, Creighton found them wanting. He sought a new set of taxonomic principles, one he eventually found in Ernst Mayr's Systematics and the Origin of Species. Mayr's ideas tied together a number of themes running through Creighton's life: the need for a revised taxonomy, the emphasis on fieldwork, and the search for a new power center for ant taxonomy after Wheeler died. Creighton's adoption of Mayr's ideas as part of his professional identity also had very real implications for his career path: field studies required long and intensive studies, and Creighton would always be a slow worker. His method of taxonomy contrasted sharply not only with Wheeler's but also with two of his younger colleagues, William L. Brown and E. O. Wilson, who took over Wheeler's spot at Harvard in 1950. The disputes between these men over ant taxonomy involved, in addition to questions of technical interest, questions about where and how best to do taxonomy and who could speak withthe most authority. Creighton's story reveals how these questions are interrelated. The story also reveals the importance of Mayr's book for changes occurring in taxonomy in the middle of the twentieth century.
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  • 7
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    Sciences of soils 5 (2000), S. 10-21 
    ISSN: 1432-9492
    Keywords: Soil temperature ; Triticum aestivum ; Stubble retention ; Nitrogen ; Early growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Early growth and development are often lower when wheat is sown into standing stubble. A study was conducted to determine whether this difference in early growth could be explained by the effects of stubble on soil temperature in the vicinity of the young plant. The roles of nitrogen nutrition and soil strength were also assessed. Three crops were monitored (1990–1992), with the wheat being sown into either standing wheat stubble after a no-till fallow (NT), or into no-tilled plots from which the stubble had been removed by burning (NB). Measurements were made of wheat growth and development, soil and plant N, soil temperature and penetration resistance. The site was on a black earth near Warialda in the northern wheatbelt of New South Wales, Australia. In 1992 wheat was also grown under simulated stubble to isolate the shading and soil temperature effects of stubble from other factors. A significant (P〈0.05) relationship was found between average soil temperature and above ground dry matter (DM) at 65 days after sowing (DAS) but not at 107 DAS. This relationship accounted for differences in DM production at 65 DAS between NT and NB treatments in 1991 and 1992, but not in 1990. In that year the lower DM production in NT plots was associated with poorer N nutrition, and possibly disease. Laboratory incubations indicate that immobilisation of N as stubble decomposed could have contributed to this. Burning stubble produced no immediate increase in soil N availability, so that it is unlikely that N contained in stubble contributed to the difference. Soil strength differences between treatments and phytotoxic effects are unlikely to have contributed to growth differences in this soil.
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  • 8
    ISSN: 1432-2242
    Keywords: Key words Glutenin ; Triticum aestivum ; Wheat storage proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Low-molecular-weight (LMW) glutenin subunits consist mainly of two domains, one at the N- terminus which contains repeats of short amino-acid motifs, and a non-repetitive one rich in cysteine, at the C- terminal region. In previous reports, polyacrylamide-gel electrophoresis has been used to show that large size variation exists among LMW and HMW glutenin subunits, and it has been suggested that deletions and insertions within the repetitive region are responsible for these variations in length. In this study, PCR-amplification of genomic DNA (Triticum aestivum variety Chinese Spring) was used to isolate three full-length LMW glutenin genes: LMWG-MB1, LMWG-MB2 and LMWG-MB3. The deduced amino-acid sequences show a high similarity between these ORFs, and with those of other LMW glutenin genes. Comparisons indicate that LMWG-MB1 has probably lost a 12-bp fragment through deletion and that LMWG-MB1 and LMWG-MB2 have an insertion of 81 bp within the repetitive domain. The current study has shown direct evidence that insertions and/or deletions provide a mechanistic explanation for the allelic variation, and the resultant evolution, of prolamin genes. Single-base substitutions at identical sites generate stop codons in both LMWG-MB2 and LMWG-MB3 indicating that these clones are pseudogenes.
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  • 9
    ISSN: 1432-2242
    Keywords: Key words Wheat ; High-molecular-weight glutenin ; AS-PCR ; Glu-A1 locus ; Null allele ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The present work reports new PCR markers that amplify the complete coding sequence of the specific alleles of the high molecular weight (HMW) glutenin genes. A set of AS-PCR molecular markers was designed which use primers from nucleotide sequences of the Glu-A1 and Glu-D1 genes, making use of the minor diffeences between the sequences of the x1, x2* of Glu-A1, and the x5 and y10 of Glu-D1. These primers were able to distinguish between x2* and the x1 or xNull of Glu-A1. Also x5 was distinguishable from x2, and y10 from y12. The primers amplified the complete coding regions and corresponded to the upstream and downstream flanking positions of Glu-A1 and Glu-D1. Primers designed to amplify the Glu-A1 gene amplified a single product when used with genomic DNA of common wheats and the xNull allele of this gene. This work also describes the cloning and characterisation of the nucleotide sequence of this allele. It possesses the same general structure as x2* and x1 (previously determined) and differs from these alleles in the extension of the coding sequence for a presumptive mature protein with only 384 residues. This is due to the presence of a stop codon (TAA) 1215-bp downstream from the start codon. A further stop codon (TAG), 2280-bp downstream from the starting codon is also found. The open reading frame of xNull and x1 alleles has the same size in bp. Both are larger than x2* which shows two small deletions. The reduced size of the presumptive mature protein encoded by xNull could explain the negative effect of this allele on grain quality.
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  • 10
    ISSN: 1432-2242
    Keywords: Keywords AFLPs ; Bulked segregant analysis ; Marker-assisted selection ; Microsatellites ; Powdery mildew resistance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Molecular markers were identified in common wheat for the Pm24 locus conferring resistance to different isolates of the powdery mildew pathogen, Erysiphe graminis DM f. sp. tritici (Em. Marchal). Bulked segregant analysis was used to identify amplified fragment length polymorphism (AFLP) markers and microsatellite markers linked to the gene Pm24 in an F2 progeny from the cross Chinese Spring (susceptible)× Chiyacao (resistant). Two AFLP markers XACA/CTA-407 and XACA/CCG-420, and three microsatellite markers Xgwm106, Xgwm337 and Xgwm458, were mapped in coupling phase to the Pm24 locus. The AFLP marker locus XACA/CTA-407 co-segregated with the Pm24 gene, and XACA/CCG-420 mapped 4.5 cM from this gene. Another AFLP marker locus XAAT/CCA-346 co- segregated in repulsion phase with the Pm24 locus. Pm24 was mapped close to the centromere on the short arm of chromosome 1D, contrary to the previously reported location on chromosome 6D. Pm24 segregated independently of gene Pm22, also located on chromosome 1D. An allele of microsatellite locus Xgwm337 located 2.4±1.2 cM from Pm24 was shown to be diagnostic and therefore potentially useful for pyramiding two or more genes for powdery mildew resistance in a single genotype.
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  • 11
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    Theoretical and applied genetics 100 (2000), S. 32-38 
    ISSN: 1432-2242
    Keywords: Key words Waxy (Wx) protein ; Triticum aestivum ; Amylose content ; Starch ; Rapid Visco-Analyzer ; Swelling power ; Noodle quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Waxy (Wx) protein is a granule-bound starch synthase (GBSS) responsible for amylose production in cereal endosperm. Eight isolines of wheat (Triticum aestivum L.) having different combinations of presence and absence of three Wx proteins, Wx-A1, -B1, and -D1, were produced in order to elucidate the effect of Wx protein deficiencies on the apparent amylose content and starch-pasting properties. An improved SDS gel electrophoresis showed that ’Bai Huo’ (a parental wheat) carried a variant Wx-B1 protein from an allele, Wx-B1e. Thus, wheat lines of types 1, 2, 4, and 6 examined in this study contained a variant Wx-B1 allele and not the standard allele, Wx-B1a. The results from 3 years of experiments using 176 lines derived from two cross-combinations showed that apparent amylose content increased the least in type 8 (waxy) having no Wx proteins and, in ascending order, increased in type 5 (only the Wx-A1 protein is present) 〈type 7 (Wx-D1) 〈type 6 (Wx-B1) 〈type 3 (Wx-A1 and -D1) 〈type 4 (Wx-A1 and -B1) 〈type 2 (Wx-B1 and -D1) 〈type 1 (three Wx proteins). However, Tukey’ s studentized range test did not detect significant differences in some cases. Densitometric analysis suggested that the amylose content was related to the amount of the Wx protein in the eight types. Parameters in the Rapid Visco-Analyzer test and swelling power were correlated to amylose content. Consequently, amylose content and pasting properties of starch were determined to be influenced the most by the lack of the Wx-B1 protein, followed by a lack of Wx-D1, and leastly by the Wx-A1 deficiency, which indicated the presence of differential effects of the three null alleles for the Wx protein.
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  • 12
    ISSN: 1432-2242
    Keywords: Keywords Composite populations ; Triticum aestivum ; Blumeria (Erysiphe) graminis f. sp. tritici ; Residual resistance effects ; Quantitative resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The evolution of adult plant resistance towards powdery mildew (caused by Blumeria graminis f. sp. tritici) was investigated in 11 wheat populations cultivated for 10 years in a French network for dynamic management (DM) of wheat genetic resources. The aims of the study were to compare the evolution of resistance in sites submitted to different powdery mildew pressure and to investigate the implication of specific resistance gene action in adult plant resistance. For this, 7 of the 11 populations were characterized for their composition of specific resistance genes (results presented in a former paper). Even though no population differed significantly from the initial PA0 pool for mean adult plant resistance, divergence appeared among the final populations. The populations with the highest adult plant resistance level originated from sites where powdery mildew pressure is known to be high (Vervins, Le Rheu), whereas populations with the lowest adult plant resistance corresponded to areas with no, or very low, powdery mildew pressure (Toulouse, Montreuil-Bellay). A residual effect of defeated specific resistance genes was hypothesized, as lines accumulating at least two specific resistance genes appeared more resistant. Additional quantitative resistance seemed to be involved in adult plant resistance. DM lines appeared then as an interesting source of variability for resistance towards powdery mildew. Moreover, as these lines had been grown in mixed populations they may be appropriate as components of a composite cultivar.
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  • 13
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    Theoretical and applied genetics 100 (2000), S. 519-527 
    ISSN: 1432-2242
    Keywords: Key words Wheat ; Triticum aestivum ; Physical mapping ; Deletion lines ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Extended physical maps of chromosomes 6A, 6B and 6D of common wheat (Triticum aestivum L. em Thell., 2n=6x=42, AABBDD) were constructed with 107 DNA clones and 45 homoeologous group-6 deletion lines. Two-hundred and ten RFLP loci were mapped, including three orthologous loci with each of 34 clones, two orthologous loci with each of 31 clones, one locus with 40 clones, two paralogous loci with one clone, and four loci, including three orthologs and one paralog, with one clone. Fifty five, 74 and 81 loci were mapped in 6A, 6B and 6D, respectively. The linear orders of the mapped orthologous loci in 6A, 6B and 6D appear to be identical and 65 loci were placed on a group-6 consensus physical map. Comparison of the consensus physical map with eight linkage maps of homoeologous group-6 chromosomes from six Triticeaespecies disclosed that the linear orders of the loci on the maps are largely, if not entirely, conserved. The relative distributions of loci on the physical and linkage maps differ markedly, however. On most of the linkage maps, the loci are either distributed relatively evenly or clustered around the centromere. In contrast, approximately 90% of the loci on the three physical maps are located either in the distal one-half or the distal two-thirds of the six chromosome arms and most of the loci are clustered in two or three segments in each chromosome.
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  • 14
    ISSN: 1432-2242
    Keywords: Key words Composite populations ; Triticum aestivum ; Blumeria (Erysiphe) graminis f. sp. tritici ; Selection ; Drift
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Dynamic management has been proposed as a complementary strategy to gene banks for the conservation of genetic resources. The evolution of frequencies of genes for specific resistance towards powdery mildew (caused by Blumeria graminis f. sp. tritici) in populations of a French network for dynamic management of bread wheat genetic resources was investigated after 10 years of multiplication without human selection. The objective was to determine whether specific resistance gene diversity was maintained in the populations and whether any changes could be attributed to selection due to pathogen pressure. Seven populations, originating from four of the network sites, were characterized and compared to the initial population for six specific resistance gene frequencies detected by nine Blumeria graminis f. sp. tritici isolates. Diversity decreased at the population level, but because of a strong differentiation between the populations, this diversity was maintained at the network level. The comparison of Fst parameters estimated on neutral markers (RFLP) and on resistance gene data revealed that in two of the populations specific resistance genes had been selected by pathogen pressure, whereas evolution in two other populations seemed to be the result of genetic drift. For the three last populations, conclusions were less clear, as one had probably experienced a strong bottleneck and the other two presented intermediate Fst values. A dynamic management network with sites contrasted for pathogen pressure, allowing genetic drift in some populations and selection in others, appeared, at least on the short term, to be a good tool for maintaining the diversity of genes for specific resistance to powdery mildew.
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  • 15
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    Mycoscience 41 (2000), S. 15-23 
    ISSN: 1618-2545
    Keywords: Agaricales ; Clitocybe trogioides var.odorifera ; Gerronema nemorale ; Psathyrella cineraria ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two new species and one new variety of Agaricales are described and illustrated from central Honshu, Japan:Clitocybe trogioides var.odorifera var. nov. (subgenusCystoclitus sectionCystoclitus), forming white, infundibuliform basidiomata, was collected from leaf litter in theQuercus-Pasania forests;Gerronema nemorale sp. nov. (sectionXanthophylla), forming small, olivaceous, omphalinoid basidiomata, was found on dead fallen twigs in theQuercus-Pasania forests;Psathyrella cineraria sp. nov. (subgenusMycophylla sectionArgillosporae), forming basidiomata covered by detersile, dark grey, fibrillose-squamulose veil, was found on decayed wood ofQuercus myrsinaefolia.
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  • 16
    ISSN: 1618-2545
    Keywords: Diaporthe kyushuensis ; grapevine swelling arm ; Phomopsis vitimegaspora ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diaporthe kyushuensis sp. nov. is described and named as the teleomorph of causal fungus of grapevine swelling arm. The anamorph of the fungus isPhomopsis vitimegaspora.
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  • 17
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    Mycoscience 41 (2000), S. 115-122 
    ISSN: 1618-2545
    Keywords: distribution ; Pinus ; Quercus ; taxonomy ; Uredinales
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Asian pine gall rust, which has hitherto been assigned toCronartium quercuum distributed in North America, is described asC. orientale, sp. nov. The spermogonial and aecial states occur on hard pines, and the uredinial and telial states occur on various oaks.Cronartium orientale has globose, almost hyaline basidiospores in contrast to the ellipsoid, yellow-orange ones of North AmericanC. quercuum s.l. Characteristics of the new rust species in morphology, DNA analysis, and host alternation are discussed.
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  • 18
    ISSN: 1618-2545
    Keywords: China ; Leptographium ; morphology ; taxonomy ; Tomicus piniperda
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tomicus species (Coleoptera: Scolytidae) are serious pests of pines with a wide distribution in Europe, Asia and America. In Yunnan, south-western China,T. piniperda has destroyed more than 0.5 million ha ofPinus yunnanensis in the past 15 years. A blue stain fungus belonging to the genusLeptographium is associated with both the shoot-feeding and trunk-attacking stages of the beetle's life cycle. The fungus is morphologically similar to the anamorph ofOphiostoma crassivaginatum and toL. pyrinum, which are both characterised by short robust conidiophores and hyphae covered by a granular layer. Both these species have been isolated from conifers and are associated with insects. After comparing the fungus fromT. piniperda with similarLeptographium species, using light and scanning electron microscopy, we concluded that it represents a new taxon, which is described here asL. yunnanense sp. nov.
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  • 19
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    Russian journal of plant physiology 47 (2000), S. 734-739 
    ISSN: 1608-3407
    Keywords: Triticum aestivum ; bioelectric potentials ; frost resistance ; critical points
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Winter wheat (Triticum aestivum L.) seedlings of three cultivars differing in frost resistance were used to study cooling-induced changes in the bioelectric potential. Measurements were performed with nonfreezing graphite–glycerol electrodes in the regime of monitoring. Upon a gradual change in air temperature from 20 to –15°C at the rates of 20 and 2°C/h, the bioelectric potential underwent abrupt transitions at certain moments, indicating changes in the physiological condition of plants. The time required for the achievement of these critical states, as well as the survival of plants after thawing, depended both on the temperature and the cooling rate. Apparently, these characteristics were related to the dynamics of phase transitions of water. Cultivar-specific features were manifested in the different abilities of plants to maintain free water in a supercooled state. It is supposed that the critical points are related to the cold resistance of colloid systems and to the temperature lethal for plants.
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    Biology and philosophy 15 (2000), S. 349-388 
    ISSN: 1572-8404
    Keywords: cline ; definition ; deme ; evolutionary synthesis ; experimental taxonomy ; J. Heslop-Harrison ; J.S. Huxley ; J.S.L. Gilmour ; nominalism ; systematics ; Systematics Association ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Philosophy
    Notes: Abstract The word ``deme'' was coined by the botanists J.S.L. Gilmour and J.W.Gregor in 1939, following the pattern of J.S. Huxley's ``cline''. Its purposewas not only to rationalize the plethora of terms describing chromosomaland genetic variation, but also to reduce hostility between traditionaltaxonomists and researchers on evolution, who sometimes scorned eachother's understanding of species. A multi-layered system of compoundterms based on deme was published by Gilmour and J. Heslop-Harrison in1954 but not widely used. Deme was adopted with a modified meaning byzoologists leading the evolutionary synthesis – Huxley, Simpson, Wright,and Mayr. Connections are shown between Gilmour's ideas around definingthe deme, his role in founding the Systematics Association, and his chapter``Taxonomy and Philosophy'' in the book The New Systematics. Thishistorical episode raises questions about the role of carefully-definedwords in scientific practice.
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  • 21
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    Mycoscience 41 (2000), S. 87-89 
    ISSN: 1618-2545
    Keywords: Cortinarius elatior var.albipes ; Cortinarius Sect.Defibulati ; new variety ; taxonomy
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    Topics: Biology
    Notes: Abstract Cortinarius elatior var.albipes var. nov., found in the deciduous forest is described and illustrated. It is distinguished from the type variety by having a white stipe and longer spores. The differences betweenCortinarius elatior var.albipes and similar taxa are briefly discussed.
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    Mycoscience 41 (2000), S. 167-169 
    ISSN: 1618-2545
    Keywords: East Asia ; freshwater fungi ; taxonomy ; tropical mycology ; Xylariales
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    Topics: Biology
    Notes: Abstract Ceratostomella hyalocoronata is described and illustrated as a new species from old decaying wood immersed in a stream in Guangdong Province, southern China. It is compared toC. hyalostoma, a temperate terrestrial species.
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    Mycoscience 41 (2000), S. 379-388 
    ISSN: 1618-2545
    Keywords: arenicolous ; marine fungi ; taxonomy
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    Topics: Biology
    Notes: Abstract Caryospora australiensis sp. nov.,Platystomum scabridisporum sp. nov. andSavoryella melanospora sp. nov. are described from driftwood collected from a sand dune at Rye, on the Mornington Peninsula Nature Park, Victoria, Australia. These species are compared with other taxa in these and related genera.
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    Mycoscience 41 (2000), S. 371-377 
    ISSN: 1618-2545
    Keywords: Platygloea ; Platygloeales ; Sigmogloea ; taxonomy ; Tremellales
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    Topics: Biology
    Notes: Abstract Specimens of two heterobasidiomycetous species, collected in Ontario and studied respectively by H. S. Jackson and R. F. Cain, are described as new; they arePlatygloea jacksonii andSigmogloea tremelloidea. P. jacksonii is characterized by small basidia and basidiospores and by sympodially developing conidia.Sigmogloea tremelloidea is an anomalous taxon with cylindric, mostly transversely septate (“auricularioid”) basidia, but some features suggest a possible relationship to the Tremellales.
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  • 25
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    Mycoscience 41 (2000), S. 407-410 
    ISSN: 1618-2545
    Keywords: Cortinarius purpurascens var.largusoides ; Cortinarius herpeticus var.fageticola ; Cortinarius Sect.Scauri ; mycoflora ; taxonomy
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    Notes: Abstract Two species ofCortinarius Sect.Scauri collected in forests ofQuercus serrata andQ. mongolica var.grosseserrata, Q. serrata andFagus crenata, orQ. mongolica var.grosseserrata andF. crenata in Niigata prefecture were identified asCortinarius purpurascens var.largusoides andC. herpeticus var.fageticola, new to Japan. Macro- and microscopical descriptions of each species are given.
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  • 26
    ISSN: 1573-8604
    Keywords: galago ; taxonomy ; vocalization ; morphology ; zoogeography
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    Topics: Biology
    Notes: Abstract Previous studies have shown the taxonomic value of vocal repertoires and hand (volar) pad characteristics in the classification of cryptic nocturnal primates such as bush babies. However, no study included quantitative comparisons within the geographical range of any one species. We investigated levels of intraspecific variation in calls and hand pad characteristics of the southern lesser bush baby (Galago moholi), using the northern lesser bush baby (Galago senegalensis) for interspecific comparisons. Examination of calls recorded from different regions along a transect of 1500 km across southern Africa revealed low levels of intraspecific variation in Galago moholi, whereas comparisons with homologous call-types in G. senegalensis revealed them to be significantly different. Volar pad measurements across the ranges of both species also showed low levels of intraspecific variation and relatively high interspecific variation. These findings demonstrate that vocal and volar pad characteristics can be used as consistent measures of difference between species that look almost identical. These methods provide a practical means of distinguishing between cryptic species, whether in the field, in captivity, or, in the case of volar pads, of preserved specimens.
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  • 27
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    International journal of primatology 21 (2000), S. 963-1019 
    ISSN: 1573-8604
    Keywords: mouse lemurs ; Microcebus ; taxonomy ; revision ; new species
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    Topics: Biology
    Notes: Abstract The genus Microcebus (mouse lemurs) are the smallest extant primates. Until recently, they were considered to comprise two different species: Microcebus murinus, confined largely to dry forests on the western portion of Madagascar, and M. rufus, occurring in humid forest formations of eastern Madagascar. Specimens and recent field observations document rufous individuals in the west. However, the current taxonomy is entangled due to a lack of comparative material to quantify intrapopulation and intraspecific morphological variation. On the basis of recently collected specimens of Microcebus from 12 localities in portions of western Madagascar, from Ankarana in the north to Beza Mahafaly in the south, we present a revision using external, cranial, and dental characters. We recognize seven species of Microcebus from western Madagascar. We name and describe 3 spp., resurrect a previously synonymized species, and amend diagnoses for Microcebus murinus (J. F. Miller, 1777), M. myoxinus Peters, 1852, and M. ravelobensis Zimmermann et al., 1998.
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    International journal of primatology 21 (2000), S. 381-420 
    ISSN: 1573-8604
    Keywords: phylogenetics ; taxonomy ; systematic ; Ateles ; Cebidae
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    Notes: Abstract Our goal was to determine phylogenetic relationships among geographically and taxonomically distinct haplotypes of spider monkeys (Ateles) based on DNA sequence variation for the mitochondrial DNA control region and cytochrome c oxidase subunit II gene. We obtained samples from most previously recognized subspecies of Ateles, ranging from Central America throughout the Amazon Basin, to determine phylogenetic relationships among racially recognized groups. Comparison of DNA sequences using both parsimony analysis and genetic distance analysis produced phylogenetic relationships that were very similar for each genetic region. We analyzed the phylograms produced, along with associated bootstrap support, confidence probabilities, and genetic distances between taxonomic groups, to identify four monophyletic species of Ateles: Ateles paniscus, composed of haplotypes from the northeastern Amazon Basin; A. belzebuth in the southern Amazon Basin; A. hybridus, located primarily along the Magdalena River valley of Colombia; and A. geoffroyi, which includes two former species: A. geoffroyi and A. fusciceps. This arrangement is contradictory to long-held taxonomies of Ateles based on pelage variation and is similar to a recent analysis based on craniodental variation. Results of this investigation suggest patterns of gene flow, evolutionary relationships, and speciation patterns that are more plausible than previous pelage-based taxonomies, which required seemingly impossible patterns of gene flow. Conservation efforts aimed at protecting Ateles, one of the Neotropics most endangered genera, will also benefit from the findings presented in this paper.
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  • 29
    ISSN: 1573-8604
    Keywords: vocalization ; sexual advertisement ; predator advertisement ; taxonomy ; evolution ; mouse lemur ; primate ; Madagascar
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    Notes: Abstract Advertisement calls are often important noninvasive tools for discriminating cryptic species and for assessing specific diversity and speciation patterns in nature. We investigated the contribution of these calls to uncover specific diversity in nocturnal Malagasy lemurs. We compared sexual advertisement and predator advertisement calls of two mouse lemur species, western gray and eastern rufous mouse lemurs (Microcebus murinus and M. rufus, respectively) living in two contrasting habitats (dry deciduous vs. rain forest), and analyzed them statistically. Both species emitted several highly variable whistle calls in the context of predator-avoidance. Intrapopulation variation was high and overlapped interspecific variation. Sexual advertisement calls, given in the mating context, displayed a totally distinct, species-specific acoustic structure. Whereas gray mouse lemurs produced rapidly multifrequency modulated, long trill calls, rufous mouse lemurs gave slowly frequency-modulated short chirp calls. Our results suggest specific status for gray and rufous mouse lemurs and indicate the importance of predation and social needs in shaping vocal communication.
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    Plant systematics and evolution 221 (2000), S. 107-112 
    ISSN: 1615-6110
    Keywords: Rosaceae ; Rosa ; Caninae ; epicuticular waxes ; taxonomy ; hybridism
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    Topics: Biology
    Notes: Abstract Classification within the genusRosa is problematic due to ± continuous variation of morphological characters. The dogroses, (Rosa, sectionCaninae) are classified only by their unique meiotic system not by directly visible characters. The intrasectional structure remains insufficient, as the characters (glands, hairs and prickles) are known to exhibit great morphological divergence affected by both genetic plasticity and environmental influences. Taxonomy of dogroses follows a highly artificial system which allows us to identify morphospecies, which are not necessarily evolutionary species. However, SEM-studies in theCaninae revealed that all taxa of the subsectionRubiginosae are characterised by a granule type of epicuticular waxes, whereas members from the other subsections (with three exceptions) form triangular rodlets. Comparison with specimens grown under the same conditions confirmed these findings on natural populations and additionally revealed that following artificial hybridism, offspring develop the maternal type of wax structure due to the matroclinal inheritance in this section.
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  • 31
    ISSN: 1615-6110
    Keywords: Acacia ; classification ; Leguminosae ; morphology ; phenetics ; RAPD ; software ; taxonomy ; UPGMA
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    Topics: Biology
    Notes: Abstract The phenetic analysis of non-nodulatingAcacia species by Harrier et al. (1997) was repeated to illustrate how different computer programs may generate alternative UPGMA trees for the very same data, even in the absence of data input order effects (ties). For example, all Harrier et al.'s UPGMA dendrograms produced by software from the Scottish Agricultural Statistics Service differed from those obtained by the packages NTSYS and MVSP87. Particularly, the positions ofA. albida, A. rovumae, andA. pentagona, as well as the relationships betweenDiacanthae andTriacanthae were affected by this phenomenon. Hence, whenever clustering techniques are used, care should be taken to consider possible software-dependent caveats and artefacts. Nevertheless, all programs provided clusterings that largely coincided with the subgeneric and sectional groupings proposed by Vassal (1972) although the positions of some species varied depending on whether morphological or molecular data were considered (e.g.A. albida andA. rovumae).
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  • 32
    ISSN: 1615-6110
    Keywords: Leguminosae ; Papilionoideae ; Lonchocarpus ; leaflets ; secretory cavities ; false secretory cavities ; pellucid dots ; glands ; anatomy ; taxonomy
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    Notes: Abstract The occurrence of leaflet secretory cavities in 23 Brazilian species ofLonchocarpus was surveyed anatomically and compared with data from external morphology (presence of pellucid dots) to evaluate their taxonomic significance. This study revealed three cases: presence of secretory cavities associated with pellucid dots, pellucid dots corresponding to false secretory cavities, and absence of pellucid dots and secretory cavities. These results indicate that in this genus the “glandular pellucid dots” cited in morphological descriptions do not always correspond to secretory cavities, and that their nature must always be confirmed by histological sections. Secretory cavities have systematic significance at subgeneric taxonomic level for the genusLonchocarpus.
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    Plant systematics and evolution 224 (2000), S. 173-182 
    ISSN: 1615-6110
    Keywords: Gesneriaceae ; Streptocarpus ; Chromosome numbers ; growth patterns ; taxonomy ; Africa ; Comoro Islands ; Madagascar
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    Topics: Biology
    Notes: Abstract This study records the chromosome numbers of 10 species ofStreptocarpus; nine of the counts are new. With the exception ofS. buchananii of mainland Africa, all the results are for plants endemic to Madagascar and the Comoro Islands. While there is a strong correlation between basic number and growth form in the two subgenera of the genus on the African mainland (x = 15 among caulescent species in subgenusStreptocarpella; x = 16 among acaulescent species in subgenusStreptocarpus), the situation appears more complex among Madagascan and Comoro Island species. One notable example of deviation from this correlation is shown byS. papangae, a shrubby caulescent species, with 2n = 32 (x = 16). Polyploidy in the genus appears to be absent on mainland Africa, but is present in Madagascar and the Comoro Islands, ranging from tetraploidy to octoploidy. Evolutionary implications of the cytological observations are considered.
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    Plant systematics and evolution 225 (2000), S. 85-101 
    ISSN: 1615-6110
    Keywords: Elaeagnaceae ; Hippophae ; sea buckthorn ; Systematics ; taxonomy ; genetic variation ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic diversity has been investigated by the application of molecular markers in, for the first time, all the taxa recognised in recent treatises of the genusHippophae. RAPD (random amplified polymorphic DNA) analyses were conducted with 9 decamer primers, which together yielded 219 polymorphic markers. We found 16 fixed RAPD markers, i.e. markers that either occurred in all plants of a population or were absent from all plants. Several of these markers were useful for analysis of interspecific relationships, whereas others can be considered as taxon-specific markers. Clustering of taxa and populations in our neighbour-joining based dendrogram was in good agreement with some recently suggested taxonomic treatises ofHippophae. Amount and distribution of genetic variability varied considerably between species. Partitioning of molecular variance withinH. rhamnoides supported earlier findings that a considerable part of the total variance resides among subspecies (59.6%) Within-population variability also differed considerably. Percentage polymorphic RAPD loci and Lynch and Milligan within-population gene diversity estimates showed relatively high values for some species close to the geographic centre of origin in Central Asia, e.g.H. tibetana and the putatively hybridogenousH. goniocarpa. Spatial autocorrelation analyses performed on 12 populations ofH. rhamnoides revealed positive autocorrelation of allele frequencies when geographic distances ranged from 0 to 700 km, and no or negative autocorrelation at higher distances. At distances between 700 and 1900 km, we observed deviations from the expected values with strongly negative autocorrelation of allele frequencies. A corresponding relationship between geographic and genetic distances could not be found when the analysis instead was based on one population from each of 8 species.
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    Plant and soil 226 (2000), S. 11-19 
    ISSN: 1573-5036
    Keywords: Chlorpropham (CIPC) ; microtubules ; nuclei ; recovery ; roots ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The present ultrastructural investigation on the effects of 50 μM chlorpropham (previously called CIPC) on growing roots of wheat (Triticum aestivum (L.) Thell cv. Vergina) was undertaken to clarify the mechanism of a carbamate herbicide action in plant cells, since the wide range of responses of plant cells to carbamate herbicides is based mainly on immunofluorescence studies. Cells of control roots contained abundant microtubules both in interphase and mitotic arrays. In chlorpropham-treated roots, however, no microtubules could be detected at all, neither in dividing nor in differentiating cells. Cycling cells became binucleate, polyploid or contained incomplete cell walls, the result of inhibition of cytokinesis. In long-term drug treatments (24 h or more) the affected cells entered a new cycle, which, however, did not progress beyond mid-metaphase. The nuclei of binucleate cells initiated prophase synchronously. Small vacuoles and Golgi vesicles were trapped within the nucleoplasm of the multilobed nuclei. In roots recovering from 8 h chlorpropham treatment, cells continued to exhibit polyploid nuclei, intranuclear vacuoles and incomplete walls. Microtubules reappeared but they were sparse and lacked a definite orientation. Preprophase cells did not form normal preprophase bands of microtubules, while mitotic cells occasionally contained microtubules bound to chromosomes and converged to minipoles. It is concluded that chlorpropham disorganized directly microtubules in addition to irreversibly affecting microtubule organizing centres, which failed to further support microtubule arrays.
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    Plant and soil 226 (2000), S. 275-285 
    ISSN: 1573-5036
    Keywords: arbuscular mycorrhiza ; Daucus carota ; Glomus mosseae ; Glomus intraradices ; monoxenic culture ; N uptake ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract New information on N uptake and transport of inorganic and organic N in arbuscular mycorrhizal fungi is reviewed here. Hyphae of the arbuscular mycorrhizal fungus Glomus mosseae (Nicol. and Gerd.) Gerd. and Trappe (BEG 107) were shown to transport N supplied as 15N-Gly to wheat plants after a 48 h labelling period in semi-hydroponic (Perlite), non-sterile, compartmentalised pot cultures. Of the 15N supplied to hyphae in pot cultures over 48 h, 0.2 and 6% was transported to plants supplied with insufficient N or sufficient N, respectively. The increased 15N uptake at the higher N supply was related to the higher hyphal length density at the higher N supply. These findings were supported by results from in vitro and monoxenic studies. Excised hyphae from four Glomus isolates (BEG 84, 107, 108 and 110) acquired N from both inorganic (15NH4 15NO3, 15NO3 − or 15NH4 +) and organic (15N-Gly and 15N-Glu, except in BEG 84 where amino acid uptake was not tested) sources in vitro during short-term experiments. Confirming these studies under sterile conditions where no bacterial mineralisation of organic N occurred, monoxenic cultures of Glomus intraradices Schenk and Smith were shown to transport N from organic sources (15N-Gly and 15N-Glu) to Ri T-DNA transformed, AM-colonised carrot roots in a long-term experiment. The higher N uptake (also from organic N) by isolates from nutrient poor sites (BEG 108 and 110) compared to that from a conventional agricultural field implied that ecotypic differences occur. Although the arbuscular mycorrhizal isolates used contributed to the acquisition of N from both inorganic and organic sources by the host plants/roots used, this was not enough to increase the N nutritional status of the mycorrhizal compared to non-mycorrhizal hosts.
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    Plant cell, tissue and organ culture 63 (2000), S. 35-40 
    ISSN: 1573-5044
    Keywords: anther culture ; EDTA ; ferrous ions ; ferric ions ; Hordeum vulgare ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A suitable form of iron supplement in the induction medium was found to be important for further development of induced pollen embryos in barley and wheat cultivars (genotypes), especially those providing few green plants viain vitro androgenesis. Genotypes able to regenerate many green plants were less susceptible to the lack of iron in induction medium. Although Fe-EDTA was found to be a suitable form of iron in the induction medium, androgenesis was also induced on media containing non-chelated iron (Fe2+ and Fe3+ ions). EDTA alone without iron inhibited the androgenic response even in the wheat cv. Florida, a model cultivar for androgenesis in wheat. In all barley cultivars under study including cv. Igri, a model cultivar for androgenesis in barley, EDTA alone caused an almost total suppression of androgenesis.
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  • 38
    ISSN: 1573-5060
    Keywords: Cheyenne ; polymorphism ; RAPD ; recombinant inbred chromosome line(RICL) RFLP ; STS ; SSR ; Triticum aestivum ; Wichita
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Previously chromosome 3A of wheat (Triticum aestivum L.) was reported to carry genes influencing yield, yield components, plant height, and anthesis date. The objective of current study was to survey various molecular marker systems for their ability to detect polymorphism between wheat cultivars Cheyenne(CNN) and Wichita (WI), particularly for chromosome3A. Seventy-seven `sequence tagged site' (STS), 10simple sequence repeat (SSR), 40 randomly amplified polymorphic DNA (RAPD) markers, and 52 restriction fragment length polymorphism (RFLP) probes for wheat homoeologous group 3 chromosomes, were investigated. Three (3.9%) STS-PCR primer sets amplified polymorphic fragments for the two cultivars, of which one was polymorphic for chromosome 3A. Sixty percent of SSR markers detected polymorphism between CNN and WI of which 50% were polymorphic for chromosome 3A. Twenty percent of RAPD markers detected polymorphism between CNN and WI in general, but none of these detected polymorphism for chromosome 3A. Of the fifty-two RFLP probes, 78.8% detected polymorphism between CNN and WI for group 3 chromosomes with one or more of seven restriction enzymes and 42% of the polymorphic fragements were for chromosome 3A. These high levels of RFLP and SSR polymorphisms between two related wheat cultivars could be used to map and tag genes influencing important agronomic traits. It may also be important to reconsider RFLP as the most suitable marker system at least for anchor maps of closely related wheat cultivars.
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  • 39
    ISSN: 1573-5060
    Keywords: complementary genes ; hybrid necrosis ; spring wheat ; Triticum aestivum ; winter wheat ; winter × springwheat hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The distribution and allelic expressivity of hybrid necrosis genes (Ne 1 and Ne 2) were studied in 21 winter (mostly exotic) and 43 spring type elite wheat genotypes, by crossing them with two known testers, C 306 (Ne 1-carrier) and HD 2380 (Ne 2-carrier).Ne 1 gene was present in one north-west Himalayan winter wheat landrace, Shoure Local, but absent in the other winter as well as spring wheats. Ne 2 gene was prevalent to a much lower extent in the exotic winter wheat germplasm (31.57%) as compared to the recently developed Indian and Mexican spring wheat semidwarfs (69.80%). This may suggest that breeders have tried to preclude hybrid necrosis by selecting for non-carrier genotypes in the development of exotic winter wheats in contrast to the situation in spring wheats. Based on the degree of expression of hybrid necrosis genes in the F1 hybrids, the carrier genotypes were characterized with respect to the allelic strength of the hybrid necrosis genes. The 27 non-carrier genotypes of the two ecotypes identified in the present study have a greater potential use in future hybridization programmes so as to overcome the problem of hybrid necrosis.
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  • 40
    ISSN: 1573-5060
    Keywords: allelic variation ; Chinese wheat ; glutenin subunit ; seed storage protein ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Variation in the electrophoretic banding patterns of high-molecular-weight (HMW) glutenin subunits of 274hexaploid wheat (Triticum aestivum) varieties from China was examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and 27 different major HMW glutenin subunits were identified. Each variety contained three to five subunits and 29different glutenin subunit patterns were observed in274 Chinese hexaploid wheats. Seventeen alleles were identified based on the comparison of subunits mobility with that previously identified in a set of standard hexaploid wheats. The Chinese hexaploid wheats exhibited allelic variation in HMW glutenin subunit composition and the variation differed from that of Japanese and hexaploid wheats of other countries.
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  • 41
    ISSN: 1573-5060
    Keywords: bunt infection ; bunt resistance ; frost resistance ; Tilletia caries ; T. foetida ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In order to determine the effects of bunt inoculation on frost resistance and winter hardiness in lines containing resistance genes, the bunt [Tilletia foetida (Wallroth) Liro, T. caries (DC.) Tulasne] susceptibility of wheat lines containing bunt resistance genesBt1 to Bt10 and the effect of the year on the degree of infection were studied over six years from 1991 to 1997 in an artificial inoculation nursery. Uninoculated and artificially inoculated wheat plants were tested for frost resistance in the phytotron in 1995 and in the field in boxes in three years from 1994/95 to 1996/97. The line withBt10 was very resistant, lines with Bt5, Bt6, Bt8 and Bt9 were resistant, the line with Bt4 was moderately resistant, those with Bt2 and Bt3 were moderately susceptible, the line with Bt1 was susceptible and the line with Bt7 was very susceptible to the local bunt population in Hungary. Bunt incidence also varied over years. The frost resistance of the Bt lines was generally lower after bunt inoculation than that of uninoculated plants. The increased frost kill in inoculated plants was not correlated with the extent of varietal susceptibility to bunt. Some lines with resistance, namely those with Bt5 (1.6% infection), Bt8 (0.6%) and Bt10 (0.0%), suffered significantly greater frost kill in the young plant stage as the result of bunt inoculation. By contrast, the Bt7line had excellent frost resistance and winter hardiness but suffered the greatest extent of bunt infection, whereas the Bt6 line had good frost resistance and good bunt resistance.
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    Plant and soil 222 (2000), S. 25-34 
    ISSN: 1573-5036
    Keywords: deficiency ; genotypic differences ; iron ; nutrient efficiency ; phytosiderophore ; tolerance ; Triticum aestivum ; Triticum durum ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tolerance to Zn deficiency in wheat germplasm may be inversely related to uptake and transport of Fe to shoots. The present study examined eight bread (Triticum aestivum) and two durum (T. turgidum L. conv. durum) wheat genotypes for their capacity to take up and transport Fe when grown under either Fe or Zn deficiency. Bread wheat genotypes Aroona, Excalibur and Stilleto showed tolerance to Zn and Fe deficiency, while durum wheat genotypes are clearly less tolerant to either deficiency. Roots of bread wheats tolerant to Zn deficiency exuded more phytosiderophores than sensitive bread and durum genotypes. Greater amounts of phytosideophores were exuded by roots grown under Fe than Zn deficiency. A relatively poor relationship existed between phytosiderophore exudation or the Fe uptake rate and relative shoot growth under Fe deficiency. At advanced stages of Zn deficiency, genotypes tolerant to Zn deficiency (Aroona and Stilleto) had a greater rate of Fe uptake than other genotypes. Zinc deficiency depressed the rate of Fe transport to shoots in all genotypes in early stages, while advanced Zn deficiency had the opposite effect. Compared with Zn-sufficient plants, 17-day-old Zn-deficient plants of genotypes tolerant to Zn deficiency had a lower rate of Fe transport to shoots, while genotypes sensitive to Zn deficiency (Durati, Yallaroi) had the Fe transport rate increased by Zn deficiency. A proportion of total amount of Fe taken up that was transported to shoots increased with duration of either Fe or Zn deficiency. It is concluded that greater tolerance to Zn deficiency among wheat genotypes is associated with the increased exudation of phytosiderophores, an increased Fe uptake rate and decreased transport of Fe to shoots.
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  • 43
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    Euphytica 112 (2000), S. 23-31 
    ISSN: 1573-5060
    Keywords: A granules ; B granules ; quantitative analysis ; starch quality ; triploid endosperm ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two lines of hexaploid wheat were crossed and the basic generations of parent, F1, F2 and back-cross were sown in a controlled-environment chamber. FreshF1 and back-cross grains were generated, so the material could be handled either as the standard set of basic generations on a whole-plant basis, or as an extended set on an embryo or endosperm basis. The experiment was repeated. Mature grains were harvested and the starch particle size distribution was analysed in 3284 grains from 111 plants. Means and variances were partitioned into additive, dominance and interaction components. Grains from cross-pollinations had B-granule contents between parental values, rather than of the maternal parent, indicating an involvement of the grain genotype. Quantitative models based on endosperm genotype gave a better fit to the data than those based on embryo genotype. The difference in starch B-granule content between the parents was largely due to additive genes. Dominant genes were also indicated, with the first dose in the triploid endosperm having a large effect while the second dose had little or none. Non-allelic interactions were significant in the second experiment where the use of more types of backcross made them more detectable. There were also small and significant residual effects of the maternal plant in the first experiment, attributed to the vigour of the F1 mother plant and to the cytoplasm of Sunco. Narrow-sense heritability was low, between 0.05and 0.18 depending on the generation. Transgressive segregation was not found, suggesting that all alleles tending to increase the B-granule content were found in the Sunco parent and none in ME71. There was also no detectable heterosis in this character. The results show that breeding and selection for a low B-granule content should be possible but a further reduction will require new and complementary genes.
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  • 44
    ISSN: 1573-5060
    Keywords: disease resistance ; doubled haploid ; Fusarium headblight ; genetic analysis ; Fusarium graminearum ; recombinant inbred ; Triticum aestivum ; wheat scab
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic constitution of resistance to Fusarium head blight (FHB, scab) caused by Fusarium graminearum in the Chinese wheat cultivar Sumai 3 and the Japanese cultivar Saikai 165 was investigated using doubled haploid lines (DHLs) and recombinant inbred lines (RILs). Frequency distributions of DHLs derived from two F1 crosses, Sumai 3 (very resistant to resistant; VR-R) / Gamenya (very susceptible; VS) and Sumai 3 / Emblem (VS), fitted well to 1: 2: 1 (resistant: moderately resistant: susceptible) ratios for reaction to FHB in the field. It is suggested that the resistance of Sumai 3 is controlled by two major genes with additive effects. One of the resistance genes may be linked in repulsion to the dominant suppressor B1 for awnedness with recombination values 15.1 ± 3.3% in Sumai 3 /Gamenya and 21.4 ± 4.3% in Sumai 3 / Emblem. Saikai 165 is a Japanese resistant line derived from an F1 Sumai 3 / Asakaze-komugi (moderately resistant; MR). The data for RILs derived from the cross Emblem / Saikai 165, indicates that three resistance genes control the resistance of Saikai 165.
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  • 45
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    Euphytica 113 (2000), S. 219-225 
    ISSN: 1573-5060
    Keywords: deficiency ; genotypic variation ; iron ; nutrient efficiency ; phytosiderophore ; Triticum aestivum ; Triticum durum ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Tolerance to Fe deficiency of wheat genotypes exhibiting differential tolerance to Zn deficiency is not known, even though the relationship between Fe nutrition and differential tolerance of wheat genotypes to Zn deficiency has been hypothesised frequently. In the present experiment, eight Triticum aestivum and two T. turigidum L. conv. durum cultivars were grown in nutrient solution deficient in either Znor Fe. Three indices of tolerance to nutrient deficiency were compared: relative [(-nutrient/+nutrient) × 100] shoot growth, shoot dry weight under nutrient deficiency and relative shoot/root dry weight ratio. Genotypes Aroona, Excalibur, Stilleto and Trident were classified as tolerant to both Zn and Fe deficiency, while durum wheats Durati and Yallaroi were sensitive to Zn deficiency and moderate to sensitive to Fe deficiency. Genotypes Excalibur, Stilleto and Trident come from the same breeding programme and have the common parent (line MEC3 =Sonora64//TZPP/YAQUI54) that could have been the donor of the genes for tolerance to Zn deficiency. When Fe-deficient, all wheat genotypes were severely chlorotic but kept producing shoot and root dry matter at a relatively high rate, making the relationship between the relative shoot growth and the relative leaf chlorophyll content poor. This is the first report of wheat genotypes exhibiting multiple tolerance to Zn and Fe deficiencies.
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  • 46
    ISSN: 1573-5060
    Keywords: bread wheat ; breeding and environmental effects ; gene frequency ; geographical distribution ; hybrid necrosis ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using the Information and Analytical System of Wheat Genetic Resources GRIS 3.2, the peculiarities of distribution of hybrid necrosis genes in bread wheat in different regions of the world were analyzed. Considerable variation in frequencies of the Ne1 and Ne2 genes in regions with different moisture and heat supply was revealed. A significant effect of breeding on frequency dynamics of different genotypes Ne1ne2, ne1Ne2 and ne1ne2 was confirmed.
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  • 47
    ISSN: 1573-5087
    Keywords: abscisic acid ; drought stress ; Triticum aestivum ; wheat germ agglutinin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Expression of wheat germ agglutinin (WGA) gene inthe developing embryos of wheat (Triticumaestivum L. cv. C-306) was studied in relation toabscisic acid (ABA) accumulation under water stressconditions. Imposition of water stress resulted inelevated ABA levels in the embryos at threedevelopmental stages (18, 24 and 30 DPA). On thecontrary, the effect of drought stress on WGAaccumulation was stage dependent with significantincrease in WGA content being observed at only 24 DPA. Our results suggest that apart from ABA, otherfactors which are temporally expressed, are alsoinvolved in regulation of WGA gene expression.
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  • 48
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    Genetic resources and crop evolution 47 (2000), S. 281-284 
    ISSN: 1573-5109
    Keywords: agronomic traits ; isozymes ; landrace ; Triticum aestivum ; variation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A sample of an Argentinean landrace of wheat showed considerable variation in most of the evaluated morphological and agronomic characters. However, analyses with high molecular glutenins and two isozyme systems, known to be highly polymorphic among current cultivars, revealed very little or no variation, respectively. The large difference in the observed variation between morphoagronomic and biochemical characters is discussed.
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  • 49
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    Hydrobiologia 439 (2000), S. 91-101 
    ISSN: 1573-5117
    Keywords: Rotifera ; Bdelloidea ; Korea ; taxonomy ; biogeography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-five samples from 18 terrestrial and/or freshwater habitats in the eastern part of Korea yielded 38 new records of bdelloid rotifers, 22 of which are new to the Asian fauna. Among these Korean new records, Dissotrocha aculeata reversa Berzins and Habrotrocha plana Milne were recorded outside their type localities for the first time, and Macrotrachela bullata (Murray) was reported only from two countries after its description. Bradyscela granulosa de Koning, Habrotrocha gracilis gracilis Montet, Macrotrachela latior Donner, Philodina duplicalcar (de Koning) and P. rugosa coriacea Bryce are recorded outside Europe for the first time. The taxonomy and distribution of these rare species are discussed.
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  • 50
    ISSN: 1573-5117
    Keywords: taxonomy ; cytogenetic methods ; Cyclops kolensis ; Cyclops strenuus strenuus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Comparisons of behavior of chromosomes and characteristics of eliminated chromatin during anaphase of chromatin diminution divisions were made of Russian and German populations of Cyclops kolensis and Cyclops strenuus strenuus. Differences in cytogenetic features included timing and amount of eliminated chromatin. Differences were also marked in duration of chromatin diminution, as well as timing and location of eliminated DNA between Russian and German populations of C. strenuus strenuus. In contrast to the German population of C. strenuus, the Russian population of C. strenuus strenuus did not exhibit gonomery.
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  • 51
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    Hydrobiologia 418 (2000), S. 73-80 
    ISSN: 1573-5117
    Keywords: Rotifera ; Bdelloidea ; pictorial key ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We propose a simple, user-friendly key joined to a pictorial key to the bdelloid genera, in the attempt to make bdelloid identification more accessible to non-bdelloid-specialists. The key is mostly based on illustrations rather than on descriptions, and is accompanied by an introduction of the main features readily observable in active bdelloids.
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  • 52
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    Hydrobiologia 418 (2000), S. 169-184 
    ISSN: 1573-5117
    Keywords: ostracods ; upper lip ; taxonomy ; phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper represents the first study of the morphology of the upper lip (labrum) and hypostome of ostracods using scanning electron microscopy (S.E.M.). There is considerable variation in the upper lip morphology of the 23 species of Cypridoidea (Podocopina) ostracods used in this study. The detail of the upper lip morphology of each species is very distinctive, so that species determination can be made on this feature alone, but it is not useful in diagnosing genera or subfamilies. The hypostome is not readily studied due to the large amounts of dense pseudochaetae (small, setae-like projections) protruding from it and hence is considered not to be a useful taxonomic feature. Several features of the upper lip and mouth region are documented for the first time. Comparisons of the general morphology of the upper lips of Recent ostracods with the upper lip of the fossil ostracod Pattersoncypris micropapillosa Bate, 1972, indicate that there has been very conservative evolution in these features since the Cretaceous.
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  • 53
    ISSN: 1573-5117
    Keywords: new species ; Ryocalanoid copepod ; taxonomy ; benthopelagic ; Sagami Bay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new Ryocalanoid copepod, Ryocalanus spinifrons, collected by the MTD net system at a depth of 1400 m from the southwestern part of Sagami Bay, Japan, is described. The new species is morphologically very close to R. infelix Tanaka, 1956 (female unknown) from the Izu region of Sagami Bay. It is distinguished from other species by the presence of 12 long spinules on the ventral inner side of the fifth pedigerous somite, nine setae on the coxal epipodite of the maxillule and nine large robust spinules on the coxal segment of the fourth leg. The row of five robust spines on the paragnath distinguishes R. spinifrons.
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  • 54
    ISSN: 1573-5117
    Keywords: copepodid instars ; Eucyclopinae ; taxonomy ; morphological characters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Morphological characters useful for taxonomic identification of older copepodid instars of the subfamily Eucyclopinae were studied among 14 species of Eucyclops, Macrocyclops, Ectocyclops, Paracyclops and Tropocyclops known from European Russia. For taxonomic analysis, the following elements of copepodid morphology were chosen: armament and proportion of furcal rami; morphology of swimming legs and reduced 5th and 6th legs; antennule segmentation; and relative body length of copepodid instars in comparison with the female length. Changes in morphology of major copepodid instars of the subfamily Eucyclopinae during ontogenetic development are traced and noticeable differences among five genera and 14 species are described. These differences among major copepodid stages may be important for both taxonomic and ecological analysis. For taxonomy, they provide information on development of sexes and species during maturation. For ecology they allow identification of the specimens at 4–5 copepodid instars in diapause. A key to major copepodid instars of the species from the subfamily Eucyclopinae which are known from limnetic habitats of the European part of Russia is presented.
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  • 55
    ISSN: 1573-5117
    Keywords: morphological variation ; fluctuating asymmetry ; taxonomy ; Copepoda ; Cyclopidae ; Acanthocyclops signifer ; Lake Baikal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Use of traditional methods for morphological studies only permits the analysis of a small part of the information embodied in morphological structures. Besides comparing populations using the mean values of characters which allows one to estimate their morphological similarity, analysis of variation among individuals within a population can be informative. Variation among individuals consists of factorial and stochastic components. The factorial component is an upper estimate of genetic heterogeneity and thus permits one to evaluate the population's adaptability. The stochastic component (estimated by fluctuating asymmetry, i.e. random deviations from perfect bilateral symmetry), being a measure of developmental stability, is an indicator of a population's fitness. Assessment of measurement error is necessary for assessment of the true value of the stochastic component and for selection of the most informative characters. Such analysis allows one to extract additional information from morphological data in comparison with methods traditionally used on copepods. This approach was applied to an analysis of morphological variation in the study of the Baikalian endemic cyclopoid Acanthocyclops signifer (Mazepova) from three different isolated localities. Characters typically used in studies of taxonomy of this group are considered here. Measurement error was rather high (more than 50% of the stochastic component), which can be explained by technical difficulties of measuring the characters. All populations differ in the mean values of the characters. This shows the taxonomic heterogeneity of this group and reveals the necessity of its taxonomic revision. Populations also differ in the level of stochastic and factorial components of the total variance. The data are interpreted from the point of view of taxonomy and the possible evolution of the group.
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  • 56
    ISSN: 1573-5117
    Keywords: Harpacticoida ; new species ; redescription ; taxonomy ; troglobitics ; trans-Atlantic distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nitocrellopsis texana n. sp. found in samples collected by SCUBA divers in Honey Creek Cave (Texas, U.S.A.) is described. This species is the first representative of this stygobitic taxon from a Northern American locality. The original description of N. ioneli (Dumont & Decraemer, 1975) is amended and the closely related N. ahaggarensis n. sp. is described from a well near Tamanghasset in the Ahaggar mountains in Algeria. The monophyly of Nitocrellopsis is discussed and its present day distribution is analyzed.
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  • 57
    ISSN: 1573-5117
    Keywords: dinoflagellates ; Prorocentrum ; morphology ; taxonomy ; Mexican Pacific ; red tides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We surveyed the dinoflagellate genus Prorocentrum Ehrenberg in Mexican Pacific waters, where it is rather common and sometimes causes red tides in coastal areas or shrimp farms. Material collected from Baja California and the Gulf of California was analyzed. Thirteen species were identified, all of them planktonic (although P. mexicanum is also epiphytic). All species are described by light microscopy, and most are also described by scanning electron microscopy; comments on morphology, taxonomy and distribution are made. Red tides were caused by P. dentatum, P. minimum and P. triestinum. Prorocentrum mexicanum and P. minimum were suspected of being toxic. Four species, previously reported in the Gulf of California, were not found. A total of 18 species, including the new records P. dactylum and P. lebourae have been to date reported from the Mexican Pacific.
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  • 58
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    Hydrobiologia 441 (2000), S. 63-71 
    ISSN: 1573-5117
    Keywords: Mongolia ; Eucyclops dumonti ; taxonomy ; zooplankton ; spring fauna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eucyclops dumonti sp.nov. is described from a shallow spring-fed lake in Central Mongolia. Data on its morphological variability are given, a comparison with the type population of Eucyclops serrulatus (Fischer, 1853) from St. Petersburg area is made, and its position relative to some closely related congeners is discussed.
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  • 59
    ISSN: 1573-5117
    Keywords: Diaphanosoma dubium ; D. pseudodubium sp.nov. ; morphology ; taxonomy ; geographical distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The poorly known D. dubium Manuilova is redescribed on extensive material from more than 80 populations from the Russian Far East, Central Asia, Mongolia, China, Japan, Vietnam, the Philippines, Thailand, Malaysia, Bangladesh, India and Sri Lanka. Its morphological variability and geographical distribution are analysed and supplemented by notes on biology. The conspecificity of morphologically different distant populations is discussed. In the northern part of its range, D. dubium was long confused with D. brachyurum or D. leuchtenbergianum, and in the south with D. modigliani. It seems that the recent appearance of D. dubium in fish ponds of Uzbekistan and Kazakhstan was an introduction, together with Far Eastern fish. D. pseudodubium sp.nov., close to but markedly different, is described from two lakes in the lower Amur river system. D. dubium, D. pseudodubium, D. tropicum and D. modigliani s. str. form a group of related species, widely distributed in Eastern and Southern Asia.
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  • 60
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    Hydrobiologia 420 (2000), S. 73-90 
    ISSN: 1573-5117
    Keywords: sibling species ; taxonomy ; DNA ; electrophoresis ; allozymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The tools of molecular genetics have enormous potential for clarifying the nature and age of species boundaries in marine organisms. Below I summarize the genetic implications of various species concepts, and review the results of recent molecular genetic analyses of species boundaries in marine microbes, plants, invertebrates and vertebrates. Excessive lumping, rather than excessive splitting, characterizes the current systematic situation in many groups. Morphologically similar species are often quite distinct genetically, suggesting that conservative systematic traditions or morphological stasis may be involved. Some reproductively isolated taxa exhibit only small levels of genetic differentiation, however. In these cases, large population sizes, slow rates of molecular evolution, and relatively recent origins may contribute to the difficulty in finding fixed genetic markers associated with barriers to gene exchange. The extent to which hybridization blurs species boundaries of marine organisms remains a subject of real disagreement in some groups (e.g. corals). The ages of recently diverged species are largely unknown; many appear to be older than 3 million years, but snails and fishes provide several examples of more recent divergences. Increasingly sophisticated genetic analyses make it easier to distinguish allopatric taxa, but criteria for recognition at the species level are highly inconsistent across studies. Future molecular genetic analyses should help to resolve many of these issues, particularly if coupled with other biological and paleontological approaches.
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  • 61
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    Hydrobiologia 424 (2000), S. 101-108 
    ISSN: 1573-5117
    Keywords: Ceratium ; freshwater ; ecology ; morphology ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new taxon of Ceratium is described from Lake Doïrani, Greece. It is proposed as a new species and named Ceratium monoceras. It differs morphologically from known species of Ceratium by having only one horn, the apical horn. Accordingly, the vegetative cells form cysts that have only one spine. Details of its ecology and occurence are given.
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  • 62
    ISSN: 1573-5117
    Keywords: taxonomy ; marine nematodes ; Cervonema ; Laimella ; the Strait of Magellan ; Chile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Five species of Cervonema and four species of Laimella are described from the Strait of Magellan and the Beagle Channel, Chile, six species of which are new to science. Cervonema chilensisn. sp. and Cervonema hermanin. sp. are separated from other known species of Cervonema by a short cervical region (less than one head diameter from the front end to the anterior border of the amphids). Cervonema chilensisn. sp. is characterised by a tail length of 5 anal diameters with posterior half filiform; Cervonema hermani n. sp. is characterised by a tail length of 6–9 anal diameter and posterior part (75%) cylindrical. Cervonema shiaen. sp. is characterised by the cephalic seta 4 μm long, amphids 9–10 μm in diameter; spicules 16 μm long and 0.8–0.9 abd; tail 4.7–5.4 anal diameter and 50% posterior part filiform; 4–5 minute precloacal supplements. Laimella subterminatan. sp. is characterised by the subterminal position of the buccal cavity which separates it from the other species of the genus. Laimella annaen. sp. is characterised by the head diameter 9–11 μm, cephalic setae and external labial setae 9 + 5 μm long, respectively, amphids 7 μm in diameter; spicules 28–30 μm long; tail 14–17 anal diameter and posterior part (75%) filiform; 5 precloacal supplements. Laimella sandraen. sp. is very close to Laimella annaen.sp. in having similar cephalic sensilla, amphids and spicules. Laimella sandraen. sp., however, can be separated from L.annaen. sp. by the shape of head and the structure of sperm cells, the total body length and the cylindrical part of tail. Cervonema papillatum Jensen, 1988, C. tenuicauda (Stekhoven, 1950) and L. longicauda Cobb, 1920 are found in this area as well. The key of all known species of Cervonemaand Laimellais presented.
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    Hydrobiologia 428 (2000), S. 61-66 
    ISSN: 1573-5117
    Keywords: parasitic Copepoda ; taxonomy ; new species ; grey mullet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new Nipergasilus species is described from the gills of the grey mullet Valamugil cunnesius from several localities in India and Pakistan. The new species is closely related to Nipergasilus bora (Yamaguti, 1939) which is also recorded here on three species of grey mullet. The genus Nipergasilus is redefined in order to accommodate the second species.
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  • 64
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    Hydrobiologia 428 (2000), S. 1-59 
    ISSN: 1573-5117
    Keywords: Clitellata ; Oligochaeta ; Lumbriculidae ; Rhynchelmis ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Nearctic species of Rhynchelmis (Lumbriculidae) are distinguished from the Palearctic group Rhynchelmis s. str. by longitudinal muscle bands that do not curl inwards. Six new species from western North America support the existence of two major groupings within the Nearctic fauna. Species in Group 1 are distinguished from other Rhynchelmis by large penial bulbs and multiple spermathecal diverticula. Within Group 1, Rhynchelmis yakimorum n. sp., Rhynchelmis monsserratus n. sp., Rhynchelmis gustafsoni n. sp. and Rhynchelmis utahensis n. sp. differ from the related Rhynchelmis (=Sutroa) rostrata in having short penes and spermathecae with 2 short, lobed diverticula. R. monsserratus is distinguished by a single, median spermatheca, R. gustafsoni has closely appressed, median spermathecae and atria, and R. utahensis differs in structural details of spermathecae and male pores. Rhynchelmis gilensis n. sp. has a single, median spermatheca with unbranched diverticula and distinctive nephridia. Redescription of material from the type localities of both Sutroa alpestris and R. rostrata supports their combination. Group 2, corresponding in part to Rhynchelmoides, is distinguished from Group 1 mostly by characters that are inconsistent or appear plesiomorphic. Within Group 2, Rhynchelmis saxosa n. sp. closely resembles Rhynchelmis alaskana, except for the absence of lateral blood vessels in posterior segments and distribution of prostates. Rhynchelmis elrodi and Rhynchelmis glandula consistently differ in presence of ventral glands and have different distributions, so their supposed synonymy is rejected.
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  • 65
    ISSN: 1573-5117
    Keywords: marine nematodes ; Epsilonematidae ; Metepsilonema ; taxonomy ; key to species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Four new species of Metepsilonema are described from sublittoral stations from the Channel and are mainly characterized: M. volutum sp.nov. by the large body size for the genus up to 460 μm, 118–121 well overlapping annuli and wide multispiral amphids; M. amphidoxum sp.nov. by 127–133 shortly overlapping annuli and sexual dimorphism in amphid size; M. comptum sp.nov. by 133–140 non-overlapping annuli, anteriormost annuli provided with a wide lumen and borders ornamented with ridges and M. corrugatum sp.nov. by 108–111 overlapping annuli provided with ridges). Additional information is provided for M. callosum together with a discussion on intraspecific variability. A new species of Metepsilonema spec. based on females only, is described but remain unnamed. M. comptum sp.nov. and M. corrugatum sp.nov. are also occurring in the Mediterranean Sea. A polytomous key to species level is presented.
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  • 66
    ISSN: 1573-5117
    Keywords: China ; freshwater ; Nematoda ; new species ; Parodontophora ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Parodontophora limnophila sp. nov. is described from Poyang Lake, the largest freshwater lake of China. It is characterized by having an amphid with its posterior end close to the base of the stoma, relatively short cephalic setae, opisthocephalic setae arranged as two subdorsal groups of three longitudinally arranged setae and two single subventral setae, excretory pore at the level of the anterior part of the stoma and renette gland 34–47% of the oesophageal length. To date, the new species is the only Parodontophora species found in freshwater habitats.
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  • 67
    ISSN: 1573-5117
    Keywords: Harpacticoida ; ground water ; interstitial ; taxonomy ; Slovenia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genus Morariopsishas a disjunct distribution. It has been found in south-eastern Europe and in the Baikal region in Russia. In Europe two species are known, both from the Dinaric region. They have been found in three caves only, but always in puddles filled with percolating water. This indicates that they inhabit an unsaturated karstic environment. A third species, Morariopsis dumontin.sp has now been found, in the same type of habitat, on several dates, in a small cave in central Slovenia. Males were recorded for the first time in this genus. The new species is related to Morariospsis kieferiPetkovski, 1959 from Dalmatia. The main differences are in the ornamentation of the furca and of the abdominal segments. P5 and particularly endopodite of P4 in males suggest a close relation between Morariaand Morariopsis.
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  • 68
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    Hydrobiologia 437 (2000), S. 235-239 
    ISSN: 1573-5117
    Keywords: Harpacticoida ; ground water ; interstitial ; taxonomy ; Slovenia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Parastenocaris andreji n. sp. was found in ground water in a northwestern part of Slovenia (southern part of Central Europe). Two adult females of the new species were filtered out of 20 000 l of water during examination of an aquifer for water supply. The bore well was 10 m deep. The aquifer is fed by rainwater and probably only occasionally from a nearby alpine river Sava. The new species differs markedly from other known species of the genus by shape and armature of furcal rami, very long and slim maxillae and some characters on endopodites P2–P5. The closest related species are probably Parastenocaris nolliKiefer, 1938 with subspecies P. nolli alpinaKiefer, 1960, P. gertrudae Kiefer, 1968 and P. austriacaKiefer, 1976.
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  • 69
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    Journal of chemical ecology 26 (2000), S. 2141-2154 
    ISSN: 1573-1561
    Keywords: Allelopathy ; phenolic acids ; 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one ; DIMBOA ; GC-MS-MS ; wheat ; Triticum aestivum ; weed suppression ; annual ryegrass ; Lolium rigidum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Wheat allelopathy has potential for weed suppression. Allelochemicals were identified in wheat seedlings, and they were exuded from seedlings into agar growth medium. p-Hydroxybenzoic, trans-p-coumaric, cis-p-coumaric, syringic, vanillic, trans-ferulic, and cis-ferulic acids and 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) were identified in both the shoots and roots of 17-day-old wheat seedlings and their associated agar growth medium. Wheat accessions with previously identified allelopathic activity tended to contain higher levels of allelochemicals than poorly allelopathic ones. The allelopathic compounds present in the shoots generally also were identified in the roots and in the agar medium. Allelochemicals were distributed differentially in wheat, with roots normally containing higher levels of allelochemicals than the shoots. When the eight allelochemicals were grouped into benzoic acid and cinnamic acid derivatives, DIMBOA, total coumaric, and total ferulic acids, the amount of each group of allelochemicals was correlated between the roots and the shoots. Most of the allelochemicals identified in the shoots and roots could be exuded by the living roots of wheat seedling into the agar growth medium. However, the amounts of allelochemicals in the agar growth medium were not proportional to those in the roots. Results suggest that wheat plants may retain allelochemicals once synthesized. The presence of allelochemicals in the agar growth medium demonstrated that wheat seedlings were able to synthesize and to exude phytotoxic compounds through their root system that could inhibit the root growth of annual ryegrass.
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  • 70
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    World journal of microbiology and biotechnology 16 (2000), S. 869-880 
    ISSN: 1573-0972
    Keywords: Biotechnology ; ecology ; fungi ; nomenclature ; synopsis ; taxonomy ; thermophilic ; thermotolerant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In applied research work dealing with heat-tolerant fungi, currently classified into two groups: namely thermotolerants and thermophiles, information on levels of thermotolerance is generally scant. Cited binomials are often referred to as representatives of thermophilic taxa. The present contribution attempts to specify proper heat-tolerance levels of species cited in biotechnological papers of academic and applied research types published in the last four decades. This assessment integrates relevant available information concerning well defined thermotolerant taxa. Distinction between both groups of heat-tolerant fungi is a mean to optimize investigations of temperature-dependent physiological processes. The nomenclatural status of the binomials retrieved was also re-appraised following the International Code of Botanical Nomenclature. Articles of the code govern the legal validity of fungal names. The goal is to deter `ghost names' that have no status of any kind. Their use in the literature is not only a source of confusion but also hinders the preparation of sound reviews and reference documents. The intention was also to detect names which do not fulfill all criteria for a valid legal publication. Their status could then be validated if the taxonomic position of the fungus justifies this procedure. The taxonomic status of these thermotolerants was also re-examined following present-day knowledge of their respective genera. Integration of warranted taxonomic decisions in the literature of applied research is crucial. These decisions consider the status of a fungus as a valid species (proposed synonymies) or the nature of its generic affinities (name change). Strict application of these decisions severly reduces levels of heterogeneity regarding names used for the same organism. It also clarifies its generic affinities with other thermotolerant fungi. The present note is not an exhaustive assessment on the nomenclatural and taxonomic positions of known thermotolerant fungi, an ecological group for which a global document remains to be produced. It only deals with those taxa most commonly cited in the literature examined. Over 130 fungi are here considered. The group manifests a diversity of taxonomic characters since it includes members of the following systematic groupings: Oomycetes, Zygomycetes, Ascomycetes, anamorphic fungi and Holobasidiomycetes. Few new taxonomic synonyms and invalid binomials are introduced in the present contribution. The former concern the following taxa: Gilmaniella thermophila, Mucor thermoaerospora, Sporotrichum lignicola and Zalerion thermophylii. Three binomials proved to have no taxonomic status of any sort: Acremonium cellulophilum, Nodulisporium microsporum and N. thermoroseum.
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    Nutrient cycling in agroecosystems 56 (2000), S. 109-116 
    ISSN: 1573-0867
    Keywords: labelled nitrogen ; Lolium perenne ; nitrogen cycling ; root biomass ; straw ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A field trial was carried out to compare the transformations and plant uptake of urine N and S in a short-term pasture from within an arable/pasture ley rotation and a long-term pasture. Animal urine labelled with 15N and 35S was applied to microplots at both sites. These microplots were destructively sampled at various time intervals over 12 months and analysed for 15N and 35S. It is known that soil organic matter accumulates under short-term pastures compared with a long-term pasture in which accumulation and degradation are in balance. Consequently, it was hypothesised that immobilization of urine N and S is more intense in the short-term. However, in this study there was considerably less immobilization of 15N and 35S into soil organic forms under short-term pasture than long-term pasture. This was attributable to a greater pasture dry matter response to urine application under the short-term pasture (due to its inherently low N fertility) resulting in a greater plant uptake of 15N and 35S with less 15N and 35S consequently being available for immobilization. At both sites, all of the applied 35S was accounted for through plant uptake and recovery in the soil, but 21–48% of the 15N was unaccounted for and presumed to have been lost through gaseous emissions. It was concluded that accumulation of soil organic N and S under short-term pastures is likely to be attributable to turnover of plant residues (particularly root material) and does not appear to be related to immobilization in urine patches.
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    Educational studies in mathematics 43 (2000), S. 191-221 
    ISSN: 1573-0816
    Keywords: analog objects ; children's informal knowledge ; conceptual mapping ; fair shares ; partitive quotient fraction construct ; partitioning tasks ; partitioning strategies ; quantification ; taxonomy ; teaching interventions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Taxonomies for classifying children's partitioning strategies have generally focused on the contexts in which the strategies occur and whether the strategies generate fair shares. Therefore, the overall aim of this study was to increase our knowledge about young children's partitioning strategies by setting out not only to identify new partitioning strategies but also to develop a taxonomy for classifying young children's partitioning strategies in terms of their ability to facilitate the abstraction of the partitive quotient fraction construct from the concrete activity of partitioning objects and/or sets of objects. Clinical interviews were conducted with twelve purposely-selected Year Three students. Each student was presented with a unique set of realistic partitioning tasks. The paper concludes with a taxonomy for: (i) qualitatively evaluating a child's progress towards the abstraction of the partitive quotient fraction construct, and (ii) planning and implementing teaching interventions commensurate with the child's level of progress towards the abstraction of the partitive quotient fraction construct. This taxonomy provides researchers and teachers with means of better utilising children's informal partitioning strategies as the foundation upon which to further develop their understandings of the partitive quotient fraction construct.
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  • 73
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    Antonie van Leeuwenhoek 77 (2000), S. 37-42 
    ISSN: 1572-9699
    Keywords: new yeast species ; Pichia sporocuriosa ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A strain of a hitherto undescribed yeast species with a unique ascospore morphology was isolated from rambutan (Nephelium lappaceum). A description of the new species, Pichia sporocuriosa, is given.
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    Antonie van Leeuwenhoek 78 (2000), S. 123-127 
    ISSN: 1572-9699
    Keywords: Bacillus subtilis ; gyrA ; phylogeny ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Partial gyrA sequences were determined for twelve strains belonging to Bacillus amyloliquefaciens, B. atrophaeus, B. licheniformis, B. mojavensis,B. subtilis subsp. subtilis, B. subtilissubsp. spizizenii and B. vallismortis. The average nucleotide and translated amino acid similarities for the seven type strains were 83.7 and 95.1%, respectively, whereas the corresponding value for the 16S rRNA sequences was 99.1%. All of the type strains were sharply separated; the closest relationship was found between B. atrophaeus and B. mojavensis which shared a nucleotide similarity of 95.8%. Phylogenetic trees were inferred from gyrA nucleotide sequences using the neighbor-joining, Fitch–Margoliash and maximum parsimony algorithms. The test strains were divided into four groups, which generally reflected results previously reported in restriction digest and DNA-DNA hybridization studies. It is concluded from the comparative sequence analysis that the gyrA sequences provide a firm framework for the rapid and accurate classification and identification of Bacillus subtilis and related taxa.
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    Antonie van Leeuwenhoek 77 (2000), S. 71-81 
    ISSN: 1572-9699
    Keywords: DNA heterogeneity ; genome comparison ; Geotrichum ; taxonomy ; yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nutritional physiology and the growth rate of thirty-four strains representing species of Geotrichum without known teleomorph states were examined. From twenty-seven strains the mol% G+C were calculated from the DNA melting curves. The first derivatives of the melting curves of seven strains, including the type strain of Geotrichum clavatum, demonstrated the presence of two peaks, 12% away from each other; the remaining strains showed only a single broad peak. DNA homology values among strains of the former group were high, indicating their conspecificity. The strains of the latter group could be subdivided into six DNA homology groups, four of which could be identified with recognized species and two may represent novel taxa. A combined key of Geotrichum and its teleomorph states Galactomyces and Dipodascus is presented.
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  • 76
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    Experimental and applied acarology 24 (2000), S. 213-225 
    ISSN: 1572-9702
    Keywords: Psoroptes ; taxonomy ; species differentiation ; phenotype ; genotype
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The biosystematic status of mite species belonging to the genus Psoroptes Gervais, 1841 is difficult to determine by phenotypic methods and has been subject to taxonomic revisions and ongoing debate. At present, the existence of five species, P. cuniculi (Delafond, 1859), P. ovis (Hering, 1838), P. equi (Hering, 1838), P. cervinus Ward, 1915 and P. natalensis Hirst, 1919, is generally accepted. This classification is based mainly on the host species, the localization of the mites on their hosts and morphological characters of male mites. However, a critical review of the literature indicates that the features used to discriminate between the five species are not unequivocal: (a) the localization of mite populations on host animals is not completely strict, (b) the lengths of the outer opisthosomal setae of male mites, which are the main morphological features used for species discrimination, overlap between the five postulated species, and (c) host specificity cannot be deduced from results of transfer experiments. Rather, conspecificity of the members of the genus Psoroptes has to be presumed which is supported by molecular genetic analyses. On these grounds and on rules of priority P. cervinus Ward, 1915, P. cuniculi (Delafond, 1859), P. natalensis Hirst, 1919 and P. ovis (Hering, 1838) are seen as synonyms of P. equi (Hering, 1838).
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  • 77
    ISSN: 1572-9699
    Keywords: Basidiomycetous yeasts ; Kondoa aeria sp. nov. ; ribosomal DNA sequencing ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genus Kondoa Y. Yamada, Nakagawa & Banno was erected to accommodate a single taxon, K. malvinella (Fell & Hunter) Y. Yamada, Nakagawa & Banno, which was transferred from the teliospore-forming genus Rhodosporidium Banno based on pronounced differences in the 5S and 26S ribosomal RNA (rRNA) nucleotide sequences to R. toruloides Banno. In contrast with the original description, reinvestigation of K. malvinella revealed the formation of transversely septate (auricularioid) basidia that did not arise on teliospores, but formed directly on the dikaryotic mycelium. The four-celled basidia developed sterigmata on which forcibly discharged asymmetric basidiospores (ballistospores) were produced. Additionally, a new taxon emerged from the study of recent isolates, for which the name K. aeria sp. nov. is proposed. This new species produced two-celled auricularioid basidia on hyphae with incomplete clamp connections. Ballistospores arose on the basidia at the tip of sterigmata and, after ejection, germinated by budding. These observations led us to present an emended diagnosis for the genus Kondoa. Analysis of the sequence data from the D1/D2 region of the 26S rRNA gene showed a very close resemblance between K. aeria and K. malvinella in a cluster that also contained several Bensingtonia species. Taxa in this cluster share specific physiological traits and produce characteristic pinkish-cream to mauve colonies; in contrast, formation of ballistoconidia is only observed in the Bensingtonia species. Sequence data supported placement of K. malvinella and K. aeria in the `Agaricostilbum clade' of the Urediniomycetes.
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  • 78
    ISSN: 1573-5109
    Keywords: cultivated plants ; databases ; herbarium ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The herbarium collection of the N.I. Vavilov Institute of Plant Industry (WIR) contains specimens of cultivated plants, their wild relatives and weedy plants and provides ample opportunities for research. It is a unique special herbarium collection and one of those rare collections having a card index (catalog). For the catalog to be functional, it should reflect the current changes in the taxa names. Only a computerized card file may meet this requirement. The creation of a database for VIR's herbarium was launched in 1995. VIR is carrying out a range of work necessary for providing a wider access to its herbarium and related information for the international scientific community.
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  • 79
    ISSN: 1573-5109
    Keywords: evolution ; genetic resources ; RAPDs ; seed protein electrophoresis ; taxonomy ; Vicia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The genetic diversity of 58 wild and weedy populations representing taxa within the V. sativa aggregate from the former USSR, 4 cultivars of V. sativa, 2 accessions of V. cordata and 3 accessions of V. macrocarpa from Mediterranean countries were analysed using randomly amplified DNA fragments (RAPDs) and seed protein electrophoresis (SDS-PAGE). Interspecific variation between taxa in the V. sativa aggregate could readily be detected using both techniques. RAPDs and seed protein patterns were found to be an effective means of identifying accessions that cannot be identified clearly by morphological criteria alone. RAPD and seed protein analysis revealed a clear relationship between observed genetic variation of populations and their geographical distribution. Populations from each region had their own gene pools. Geographical variation was detected in V. segetalis. The degree of genetic divergence between local populations was usually related to proximity. In several locations where wild and weedy populations of different V. sativa agg. taxa grow sympatrically, intermediate forms could be detected at the DNA and protein levels. Both RAPD and seed protein analysis support the view that the V. sativa aggregate consists of 8 taxa warranting recognition at the species level. Several species in this aggregate are evolving intra-specific groups which can readily be detected at the molecular level.
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    Plant cell, tissue and organ culture 60 (2000), S. 69-73 
    ISSN: 1573-5044
    Keywords: cereals ; tissue culture ; Triticum durum ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fertile, green plants were regenerated from immature inflorescence explants from each of four Canadian wheat cultivars. The cultivars were representative of four classes of Canadian wheat. Explants from immature inflorescences of three size ranges were cultured on two types of media: MSI/MSR, which contains 1650 mg l-1 NH4NO3and sucrose as a carbon source, and BII/BIR, which contains 250 mg l-1 NH4NO3and maltose as a carbon source. Regeneration from all cultivars was significantly better on BII/BIR media than on MSI/MSR media. On BII/BIR media, `AC Karma', `Plenty', and `Fielder' gave the highest number of shoots per 10 explants, where the explants were derived from immature inflorescences 5.1 to 10.0 mm in length. 'Columbus' did not regenerate on MSI/MSR medium, and regenerated poorly on BII/BIR medium. Differences were found between cultivars with regard to the number of regenerant plants produced with the best treatments: `Plenty' produced 16.1 shoots per 10 explants, `AC Karma' 12.4, `Fielder' 6.4, and `Columbus' 2.2.
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  • 81
    ISSN: 1573-5060
    Keywords: biochemical marker ; gene location ; Lophopyrum elongatum ; Puccinia striiformis ; stripe rust ; substitution line ; Triticum aestivum ; yellow rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A set of T. aestivum-L. elongatum chromosome substitution lines was tested for yellow rust resistance at the seedling stage. Inheritance of the resistance and esterase-5 (Est-5) variation were studied. The results demonstrated that L.elongatum carried a new gene(s) conferring yellow rust resistance. This gene was dominant and located on chromosome 3E of L. elongatum. The biochemical locus encoding Est-5was also located on chromosome 3E, and co-segregated with theYr gene(s) in the wheat background. The transmission frequencies of chromosome 3E in 3E(3A) × CS, 3E(3B) × CS and 3E(3D) × CS hybrids were scored.None of the hybrids transmitted the alien chromosome at thetheoretical maximum rate, but the transmission frequencies ofchromosome 3E in F2 populations of 3E(3A) × CS and 3E(3D) × CS were significantly higher than in thatof 3E(3B) × CS.
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    Euphytica 111 (2000), S. 199-203 
    ISSN: 1573-5060
    Keywords: Diuraphis noxia (Mordvilko) ; protein ; Russian wheat aphid ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko), has become a perennial, serious pest of wheat (Triticum aestivum L.) in the western United States. Current methodologies used to enhance RWA resistance in wheat germplasm could benefit from an understanding of the biochemical mechanisms underlying resistance to RWA. This study was initiated to identify specific polypeptides induced by RWA feeding that may be associated with RWA resistance. The effects of RWA feeding on PI 140207 (a RWA-resistant spring wheat) and Pavon (a RWA-susceptible spring wheat) were examined by visualizing, silver-stained denatured leaf proteins separated by two-dimensional polyacrylamide gel electrophoresis. Comparisons of protein profiles of noninfested and RWA-infested Pavon and PI 140207 revealed a 24-kilodalton-protein complex selectively inhibited in Pavon that persisted in PI 140207during RWA attack. No other significant qualitative or quantitative differences were detected in RWA-induced alterations of protein profiles. These results suggest that RWA feeding selectively inhibit synthesis and accumulation of proteins necessary for normal metabolic functions in susceptible plants.
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  • 83
    ISSN: 1573-5036
    Keywords: plant uptake ; potassium ; potassium starvation ; radiocaesium ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Short term experiments were carried out to investigate the effect of internal tissue potassium concentration on the uptake of radiocaesium by spring wheat (Triticum aestivum cv. Tonic). The results showed that potassium starvation increased Cs influx rates by a factor of 10 compared with non-starved plants. Solution to plant tissue transfer factor (TF) values also increased by around an order of magnitude after potassium starvation treatment. The enhancement of Cs influx rates by potassium starvation could be offset by an increase in external potassium concentration: this effect is minimal at external potassium concentrations greater than approximately 200 μM (8 mg L-1). This reveals that Cs influx into plant roots is subject to control by both internal and external potassium status. The kinetics of Cs uptake by potassium in starved and non-starved plants can be described adequately by the Michaelis-Menten equation. It was shown that potassium starvation substantially reduces the Km value from approximately 28 to 6 μM, which suggests that starvation treatment increases significantly the affinity of plant roots for Cs+. Mechanisms involved in K-Cs interactions during plant uptake are discussed in this paper. Finally, the relevance of such mechanisms as determinants of radiocaesium uptake by plants growing under different ecological conditions is emphasised.
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  • 84
    ISSN: 1573-5060
    Keywords: genetic diversity ; germplasm ; RFLP ; Triticum aestivum ; wheat breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A set of 292 accessions of common wheat (Triticum aestivum L.) representing 21 germplasm pools based on geographical or breeding program origins was assayed for RFLP diversity. Thirty cDNA and genomic DNA probes and the HindIII restriction enzyme were employed for RFLP analysis. About 61% of all 233 scored bands were present in 75% or more of the accessions. All but one of the 30 probes revealed polymorphism, and the average number of distinct patterns per probe over all accessions was 9.5.Polymorphic Information Content (PIC) values within a pool varied from 0 to 0.9 and depended on the identities of both the germplasm pool and the probe. Rare banding patterns with a relative frequency of ≤0.2 within a pool were detected. These rare patterns were more likely to occur in pools exhibiting high levels of heterogeneity. The highest level of polymorphism was observed in the Turkish landraces from Southwest Asia. The Eastern U.S. soft red winter wheat germplasm pool was more genetically diverse than the other advanced germplasm pools, and nearly as diverse as the Turkish landrace pool. RFLP-based genetic relationships between germplasm pools generally tracked expectations based on common geographical origin, breeding history and/or shared parentages. The Chinese wheat landraces from Sichuan, Tibet, and Yunnan provinces were distinct from other pools. Similarity matrices for among-pool genetic distance estimates based on either band frequencies or banding pattern frequencies showed good correlation with matrices derived from Nei and Li's mean genetic similarity estimates (r=−0.82** and r=−0.73**, respectively.
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  • 85
    ISSN: 1573-5079
    Keywords: canopy architecture ; canopy photosynthesis ; CO2 enrichment ; global change ; leaf area index ; leaf tip angle ; nitrogen stress ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response of whole-canopy net CO2 exchange rate (CER) and canopy architecture to CO2 enrichment and N stress during 1996 and 1997 for open-field-grown wheat ecosystem (Triticum aestivum L. cv. Yecora Rojo) are described. Every Control (C) and FACE (F) CO2 treatment (defined as ambient and ambient +200 μmol mol−1, respectively) contained a Low- and High-N treatment. Low-N treatments constituted initial soil content amended with supplemental nitrogen applied at a rate of 70 kg N ha−1 (1996) and 15 kg N ha−1 (1997), whereas High-N treatments were supplemented with 350 kg N ha−1 (1996 and 1997). Elevated CO2 enhanced season-long carbon accumulation by 8% and 16% under Low-N and High-N, respectively. N-stress reduced season-long carbon accumulation 14% under ambient CO2, but by as much as 22% under CO2 enrichment. Averaging both years, green plant area index (GPAI) peaked approximately 76 days after planting at 7.13 for FH, 6.00 for CH, 3.89 for FL, and 3.89 for CL treatments. Leaf tip angle distribution (LTA) indicated that Low-N canopies were more erectophile than those of High-N canopies: 48° for FH, 52° for CH, and 58° for both FL and CL treatments. Temporal trends in canopy greenness indicated a decrease in leaf chlorophyll content from the flag to flag-2 leaves of 25% for FH, 28% for CH, 17% for CL, and 33% for FL during 1997. These results indicate that significant modifications of canopy architecture occurs in response to both CO2 and N-stress. Optimization of canopy architecture may serve as a mechanism to diminish CO2 and N-stress effects on CER.
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    Hydrobiologia 417 (2000), S. 1-10 
    ISSN: 1573-5117
    Keywords: taxonomy ; copepods ; Einsle ; chromatin diminution ; enzyme electrophoresis ; Cyclops
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    Hydrobiologia 421 (2000), S. 77-90 
    ISSN: 1573-5117
    Keywords: Tobrilidae ; new record ; Nematoda ; taxonomy ; China
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eutobrilus annetteae Joubert & Heyns, 1979 with its male reported for the first time, Semitobrilus pellucidus (Bastian, 1865) Tsalolichin, 1983, and Epitobrilus allophysis(Steiner, 1919) Tsalolichin, 1996 are described from the Li River at Guiling, China. The male of Eutobrilus annetteae is characterized by lots of crystalloids inside body, almost straight spicules about one anal body diameter long and seven equally sized supplements. Semitobrilus pellucidus from Guiling shows large variation in body width, position of amphids, form of buccal cavity, distance between denticles, direction of vagina, arrangement of oocytes, and shape of eggs. It is different from other populations in its shorter cephalic setae, less than 60% of head width, in its shorter tail and smaller c′ value. In males, there are 6–9 supplements, reduced and irregulary spaced. Epitobrilus allophysisis characterized by its labial setae longer than 50% of head width, small, posteriorly located ovoid amphid, single egg occupying whole space of uterus and blunt tail tip.
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    Hydrobiologia 421 (2000), S. 165-178 
    ISSN: 1573-5117
    Keywords: Kurzia ; Crustacea ; Anomopoda ; Chydoridae ; taxonomy ; distribution ; Cladocera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genus Kurzia is divided into two subgenera: Kurzia s. str. and Rostrokurzia n. subg. Subgenus Kurzia includes Kurzia (K.) latissima Kurz, 1874, with a palearctic -, Kurzia (K.) polyspina n. sp. with a neotropic - , and Kurzia (K.) cf. media (Birge, 1879) with a nearctic distribution. Rostrokurzia includes Kurzia (R.) longirostris Daday, 1898 (pantropical distribution), and Kurzia (R.) brevilabris Rajapaksa & Fernando, 1986, from subtropical and tropical Asia. Kurzia latissima Kurz, 1874, from Central Europe is redescribed in detail.
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  • 89
    ISSN: 1573-5117
    Keywords: taxonomy ; marine nematodes ; Sabatieria ; Metacomesoma ; Paramonhystera ; Siphonolaimus ; the Strait of Magellan ; Chile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Six species of Monhysteroida are described from the Strait of Magellan and the Beagle Channel, Chile. Three species of which, Sabatieria heipi n. sp., Paramonhystera geraerti n. sp. and Siphonolaimus smetti n. sp., are new to science. Sabatieria heipi n. sp. is characterised by a body length of 1529–1934 μm, cuticle punctuations with lateral differentiation; cephalic setae 5 μm long, amphids spiral with 4.0–4.2 turns, 10–11 μm in diameter; spicules 76 μm (2.1 anal body diameter), with a 6 μm projection in the ventral distal extremity, 11 minute precloacal supplements; tail 3.7–4.7 anal body diameter (abd) long with enlarged tip. Paramonhystera geraerti n. sp. is characterised by a body size of 705–767 μm; head diameter 12 μm, the cephalic setae 6 μm; amphids circular, 9–10 μm in diameter; spicules slender 108–116 μm (5.6 abd) long, 5 precloacal supplements; and tail 4.0–5.2 abd. Siphonolaimus smetti n. sp. is characterised by the long spear (40–45 μm), head diameter 11–12 μm, amphids circular, 12–13 μm in diameter or 50–57% of corresponding body diameter; the spicules 55 μm and 1.5 abd; tail conical, 2.8 abd (males) and 4.0 abd (females) long. Three other species, i.e. Metacomesoma cyatholaimoides Wieser, 1954, Paramonhystera biforma Wieser, 1956 and P. megacephala (Steiner, 1916) are redescribed. A key of all known species of Paramonhystera is proposed.
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  • 90
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    Hydrobiologia 429 (2000), S. 115-131 
    ISSN: 1573-5117
    Keywords: Mesocyclops mariae n.sp. ; Mesocyclops shenzhenensis n. sp. ; Guangxi ; Guangdong ; China ; taxonomy ; distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two new Mesocyclops species, M. mariae n. sp. and M. shenzhenensisn. sp., are described from southern China, the former species from Guangxi province, the latter from Guangdong province. Both species have setules on the medial margin of the caudal rami. Compared with each other, there are some conspicuous differences, such as the spinule pattern of the antenna basipodite, the structure of receptaculum seminis, the armature of coxopodite and basipodite of P4 and the armature of the caudal rami. The paper also compares the two new species with their supposedly closest relatives, M. pseudospinosus Dussart & Fernando, 1988, M. leuckarti(Claus, 1857) and M. pehpeiensisHu, 1943. In addition, other species of Mesocyclops in China are discussed and a key to 10 Chinese species of Mesocyclops is provided.
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  • 91
    ISSN: 1573-5117
    Keywords: acid ; taxonomy ; protists ; ciliate ; flagellate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence and some important taxonomic problems of heterotrophic protists from extremely acidic (pH ≤3) environments are briefly discussed. Almost all information on the occurrence of heterotrophic protists from extremely acidic environments is restricted to acid mine drainage (AMD) or streams influenced by AMD. Most of the information is provided for ciliates. Very little information is available on the occurrence of flagellates, rhizopods and heliozoa in this environment. Within the ciliates Urotricha, Vorticella and Oxytricha dominate in acidic mining lakes. Actinophrys sp. is the most important heliozoan in these environments. There are many taxonomic problems which are not solved so far. Live observations and taxonomic methods adapted to the extreme chemical matrix are necessary for correct identification.
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  • 92
    ISSN: 1573-5117
    Keywords: Aphanizomenon ; Cyanobacteria ; 16S rRNA ; morphology ; sequences ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The taxonomy of Aphanizomenon flos-aquae strain NH-5, a producer of cyanotoxins, was re-evaluated by comparison with six other Aphanizomenon strains using morphological characteristics and 16S rRNA gene sequences. Strain NH-5 was concluded to be improperly identified as Aph. flos-aquae based upon (1) lack of bundle formation in the trichomes, (2) location of akinetes next to heterocytes, (3) lower similarities (less than 97.5%) in the 16S rRNA gene sequences relative to Aph. flos-aquae strains, and (4) comparison within a phylogenetic tree constructed from 16S rRNA gene sequences. The Aphanizomenon strains investigated in this study are classified to four morphological groups as described by the classical taxonomy of Komárek & Kovácik (1989). This classification was supported from the phylogenetic results of 16S rRNA gene sequences. This study also discusses the generic boundaries between Aphanizomenon and Anabaena.
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  • 93
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    Hydrobiologia 438 (2000), S. 107-116 
    ISSN: 1573-5117
    Keywords: Cyanophyta/Cyanobacteria ; Aphanizomenon ; Anabaena ; taxonomy ; morphology of trichomes ; heterocytes and akinetes ; Slovakia ; Austria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Morphological variation of trichomes, heterocytes and akinetes in four species classified into the nostocalean genera Aphanizomenon Morren ex Bornet et Flahault or Anabaena Bory (Cyanophyta/Cyanobacteria) was studied from natural samples by LM and illustrated by drawings and micrographs. All investigated species, Aphanizomenon gracile (Lemmerm.) Lemmerm., A. aphanizomenoides (Forti) Komárek et Horecká, Anabaena bergii Ostenfeld, A. minderi Huber-Pestalozzi, occur in the plankton as solitary trichomes, similar to many representatives of the genus Anabaena. Main generic diagnostic characters of Aphanizomenon and Anabaena are discussed.
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  • 94
    ISSN: 1573-5117
    Keywords: Anabaena ; cyanobacteria ; identification ; morphology ; planktic ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fifty (50) strains of planktic species of Anabaena (cyanobacteria), including collections from Japan and China and from different culture collections, were induced to form akinetes at low temperature (15 °C). Their morphologies were then observed and described. Fourty seven strains successfully formed akinetes and these were classified into 20 species comprising seven with straight trichomes and 13 with coiled trichomes. Three strains, which did not form akinetes, were separated into two taxonomic groups, but could not be identified to any described species. In addition, a key to the planktic species of Anabaena described in the study is presented.
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  • 95
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    Hydrobiologia 433 (2000), S. 137-143 
    ISSN: 1573-5117
    Keywords: Cyanidium caldarium ; Cyanidioschyzon merolae ; Galdieria sulphuraria ; taxonomy ; acidophily
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ecophysiological, cytomorphological, biochemical and molecular data presently available for the acidophilic red algal species Cyanidium caldarium, Cyanidioschyzon merolae and Galdieria sulphuraria are summarised. The taxonomic position of the three genera is discussed and emendements to the generic diagnosis are presented.
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  • 96
    ISSN: 1573-5117
    Keywords: Cyanidium caldarium ; Cyanidioschyzon merolae ; Galdieria sulphuraria ; rbcL ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyanidium caldarium, Cyanidioschyzon merolae and Galdieria sulphuraria are three unicellular algae characteristic, of acid thermal environments. Recently, on the basis of morphological characters, three new species of Galdieria (G. partita, G. daedala, G. maxima ) isolated from acid-thermal springs in Russia have been instituted. A selected region of rbcL and the sequence of the intergenic spacer between the rbcL and rbcS have been amplified and sequenced from different Galdieria species and strains, in order to define molecular relationship among these interesting algae. The obtained cladogram shows that Cyanidium caldarium and Cyanidioschyzon merolae form a sister group which, in turn, is in a sister group relationship with Galdieria. This last genus is divided in two clades, one of which includes G. sulphuraria accessions from Naples (Italy), California, and Yellowstone and the other one includes G. sulphuraria accessions from Java (Indonesia) and from the Russian species. These results support the status of the genus Galdieria and suggest that G. daedala, G. maxima and G. partita are three very similar strains of G. sulphuraria; the rbcL variation within Galdieria accessions has a pattern which is broadly connected to the geographial distribution. The data obtained from the intergenic rbcL-rbcS spacer partly confirm those from the rbcL analysis.
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  • 97
    ISSN: 1573-5117
    Keywords: Chydoridae ; Anomopoda ; taxonomy ; Karualona ; trunk limbs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The species group of Alona karua is raised to generic rank and named Karualona. The genus is defined on the basis of a number of characters of the body and its appendages. Tentative new microcharacters are introduced, related to the type and length of selected setae of P1, P2, P3 and P4. Two new species are described, one from the island of Socotra and one from Mexico. A key to the five currently recognized species is given.
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  • 98
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    Hydrobiologia 435 (2000), S. 43-59 
    ISSN: 1573-5117
    Keywords: Nematoda ; Coninckiidae ; Comesomatidae ; White Sea ; taxonomy ; descriptions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ten new and known species of the genera Coninckia, Cervonema, Laimella and Sabatieria are described from the White Sea. Coninckia macroseta sp. n. resembles C. seta Wieser et Hopper, 1967 (Florida coast of U.S.A.) in shape of the amphid and other characters, but differs from the latter by greater body length (2399 μm versus 1300–1400 μm) and by the a-index (83 versus and a 57–58). Cervonema proximamphidum sp. n. differs from all other species of Cervonema by the shape of posterior widening of the pharynx (except for C. allometricum Wieser, 1954 whose original description is too brief). The pharynx of the new species forms posteriorly a conical widening with vague radial striation, whereas the pharynx of other Cervonema species presents posteriorly an oval or elongate thickening with wavy contour and distinct muscular transversal striation. The other diagnostic characters of Cervonema proximamphidum sp. n. are close position of the amphid to the anterior end (distance from the anterior end to the amphid 6–7.5 μm), longer outer labial and cephalic setae (5–5.5 μm), relatively longer and slender body (respectively, 1423–1563 μm and a=30.9–41.1). Specimens Cervonema minutus Muthumbi, Soetaert et Vincx, 1997 largely agree with the original description of Muthumbi et al. (1997) based on the nematodes sampled in the Indian Ocean deep sea; only cephalic setae length (2.7–3.5 versus 2 μm) differ. The White Sea specimens Laimella filipjevi coincide well with the description of Jensen (1979) made on nematodes from the western Baltic Sea, except slight difference in body length (1564–1837 μm versus 1086–1152 μm). Sabatieria kolaensis (Ssaweljev, 1912) is redescribed and restored as valid species. Sabatieria kolaensis was assigned by Platt (1985) to the list of dubious species because of very brief original description lacking some important details. Sabatieria kolaensis belongs to the S. praedatrix species group and is characterized by the greatest number of preanal supplements (31–40). Sabatieria lawsi Platt, 1983 was previously known from the only Antarctic Peninsula shelf; the single White Sea male specimen corresponds well to the original diagnosis of Platt (1983). Sabatieria lawsi is considered to be very close or near identical to S. praedatrix de Man, 1907. The species Sabatieria longispinosa Lorenzen, 1972, S. ornata (Ditlevsen, 1918), S. pulchra (G. Schneider, 1906) and S. strigosa Lorenzen, 1972 are also described and discussed.
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  • 99
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    Chromosome research 8 (2000), S. 671-676 
    ISSN: 1573-6849
    Keywords: meiotic metaphase I pairing ; recombinant isochromosome ; Triticum aestivum ; Triticum dicoccoides
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A recombinant isochromosome i5BLrec of wheat was developed with one arm and the proximal 36% of the other arm of Chinese Spring (CS) origin and the distal 64% of the recombined arm of Triticum turgidum subsp. dicoccoides origin. The i5BLrec} provides an unusual opportunity to analyze the role of the centromere or arm heterozygosity in chromosome prealignment and synapsis during meiosis. In monosomic condition, the i5BLrec formed a ring univalent in 86.8% of the pollen mother cells (PMCs) at meiotic metaphase I. In the disomic condition, the two i5BLrec preferentially paired as a normal bivalent in 74.8% of the PMCs, which differed significantly (p〈0.01) from the normal bivalent pairing of 51% observed in diisosomic 5BL chromosomes of the CS (Di5BLCS) control plants. In plants with one i5BLrec and a normal 5BCS, the long arm of 5BCS paired with the homologous arm of i5BLrec in 54.4% of the PMCs, and 40.4% of the PMCs had a 5BCS univalent and a i5BLrec ring univalent. The implications of the i5BLrec pairing data on the mechanism of Ph1 gene action are discussed.
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  • 100
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    Chromosome research 8 (2000), S. 501-511 
    ISSN: 1573-6849
    Keywords: BFB cycle ; chromosome healing ; gametocidal factor ; rye deficiencies ; Secale cereale ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The gametocidal factor on the Aegilops cylindrical chromosome 2Cc was used to induce and analyze the nature of chromosomal rearrangements in rye chromosomes added to wheat. For this purpose we isolated plants disomic for a given rye chromosome and monosomic for 2Cc and analyzed their progenies cytologically. Rearranged rye chromosomes were identified in 7% of the progenies and consisted of rye deficiencies (4.6%), wheat–rye dicentric and rye ring chromosomes (1.8%), and terminal translocations (0.6%). The dicentric and ring chromosomes initiated breakage–fusion–bridge cycles (BFB) that ceased within a few weeks after germination as the result of chromosome healing. Of 56 rye deficiencies identified, after backcrossing and selfing, only 33 were recovered in either homozygous or heterozygous condition covering all rye chromosomes except 7R. The low recovery rate is probably caused by the presence of multiple rearrangements induced in the wheat genome that resulted in poor plant vigor and seed set, low transmission, and an underestimation of the frequency of wheat–rye dicentric chromosomes. Genomic in-situ hybridization (GISH) analysis of the 33 recovered rye deficiencies revealed that 30 resulted from a single break in one chromosome arm followed by the loss of the segment distal to the breakpoint. Only three had a wheat segment attached distal to the breakpoint. Although some of the Gc-induced rye rearrangements were derived from BFB cycles, all of the recovered rye rearrangements were simple in structure. The healing of the broken chromosome ends was achieved either by the de-novo addition of telomeric repeats leading to deficiencies and telocentric chromosomes or by the fusion with other broken ends in the form of stable monocentric terminal translocation chromosomes.
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