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  • Ultrastructure  (348)
  • Immunocytochemistry  (149)
  • Lepidoptera  (146)
  • Angiosperms  (132)
  • Seismology
  • Springer  (774)
  • Copernicus Publications  (2)
  • Division of Earthsciences, Seismological Laboratory, California Institute of Technology
  • Dover Publ.
  • 2010-2014  (3)
  • 1980-1984  (773)
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  • 1
    facet.materialart.
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    On the multi-fractal characteristics of the ULF geomagnetic field before the 1993 Guam earthquake (2013)
    Copernicus Publications
    Details
    Publikationsdatum: 2017-04-04
    Beschreibung: Ida et al. (2005) document significant changes in the multi-fractal parameters of the ULF geomagnetic field H component starting about one month before the 1993 Guam earthquake. According to the authors, these multi-fractal signatures can be considered as precursory signals of the Guam earthquake. As a consequence, they conclude that the multifractal analysis may have an important role in the development of short-term earthquake prediction capabilities. Since this and other similar reports have motivated the idea that earthquake prediction based on electromagnetic precursory signals may one day become a routine technique, the presumed precursors need to be validated through independent datasets. In this review the seismogenic origin of the multifractal magnetic signatures documented by Ida et al. (2005) before the 8 August 1993 Guam earthquake is seriously put into question. By means of the geomagnetic ΣKp index, it is demonstrated that these multi-fractal parameter changes are normal signals induced by the variation of the global geomagnetic activity level.
    Beschreibung: Published
    Beschreibung: 187-191
    Beschreibung: 2.6. TTC - Laboratorio di gravimetria, magnetismo ed elettromagnetismo in aree attive
    Beschreibung: JCR Journal
    Beschreibung: open
    Schlagwort(e): Geomagnetic field ; Magnetic anomalies ; Earthquake precursors ; Seismology ; 04. Solid Earth::04.05. Geomagnetism::04.05.04. Magnetic anomalies
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 2
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    Comment on “Ultra Low Frequency (ULF) European multi station magnetic field analysis before and during the 2009 earthquake at L’Aquila regarding regional geotechnical information” by Prattes et al. (2011) (2012)
    Copernicus Publications
    Details
    Publikationsdatum: 2017-04-04
    Beschreibung: Prattes et al. (2011) report ULF magnetic anomalous signals claiming them to be possibly precursor of the 6 April 2009 MW6.3 L’Aquila earthquake. This comment casts doubts on the possibility that the observed magnetic signatures could have a seismogenic origin by showing that these pre-earthquake signals are actually part of normal global geomagnetic activity.
    Beschreibung: Published
    Beschreibung: 1717–1719
    Beschreibung: 2.6. TTC - Laboratorio di gravimetria, magnetismo ed elettromagnetismo in aree attive
    Beschreibung: JCR Journal
    Beschreibung: open
    Schlagwort(e): Geomagnetic field ; Earthquake precursors ; Magnetic anomalies ; Seismology ; 04. Solid Earth::04.05. Geomagnetism::04.05.99. General or miscellaneous ; 04. Solid Earth::04.05. Geomagnetism::04.05.04. Magnetic anomalies ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 3
    facet.materialart.
    Unbekannt
    Core-mantle boundary topography: seismology (2012)
    Springer
    Details
    Publikationsdatum: 2017-04-04
    Beschreibung: n/a
    Beschreibung: Published
    Beschreibung: 125-127
    Beschreibung: 3.3. Geodinamica e struttura dell'interno della Terra
    Beschreibung: restricted
    Schlagwort(e): Core-mantle boundary topography ; Seismology ; 04. Solid Earth::04.01. Earth Interior::04.01.03. Mantle and Core dynamics
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: book chapter
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  • 4
    Digitale Medien
    Digitale Medien
    Sex attractants for the fruit-attacking noctuids Orthosia incerta and Orthosia cruda (1984)
    Springer
    Entomologia experimentalis et applicata 36 (1984), S. 15-16 
    Details
    ISSN: 1570-7458
    Schlagwort(e): sex attractants ; Lepidoptera ; Noctuidae ; Orthosia incerta ; Orthosia cruda ; fruit pest ; Z9-14:Ac ; Z9-14:Ald ; Z11-16:Ac ; Z11-16:Ald
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00345198
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  • 5
    Digitale Medien
    Digitale Medien
    Factors affecting the dispersive behaviour of larvae of an Australian geometrid moth (1984)
    Springer
    Entomologia experimentalis et applicata 35 (1984), S. 159-167 
    Details
    ISSN: 1570-7458
    Schlagwort(e): Lepidoptera ; Geometridae ; Ectropis excursaria ; larval dispersal ; colour polymorphism ; phototaxis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Acht Experimente wurden durchgeführt, um einige der Faktoren zu studieren, die das Wanderverhalten der Larven (Raupen) eines polyphagen Geometriden (Ectropis excursaria (Guenée)) beein-flussen. 1. Larven sind positiv phototaktisch. Die positive Phototaxis ist negativ mit Fasten, Alter und Populationsdichte korreliert. Bei hohen Temperaturen ist sie nicht mehr nachweisbar. 2. Das Wanderverhalten der Larven wird durch die Populationsdichte beeinflußt, wodurch annähernd eine konstante Dichte erhalten bleibt. Das Verhalten der individuellen Larven is dabei nicht statistisch homogen. Es gibt ‘Wanderer’ und ‘Nicht-Wanderer’. 3. Diese Verhaltensunderschiede stehen möglicherweise im Zusammenhang mit physiologischen und morphologischen Faktoren, die den individuellen Fortpflanzungserfolg und das überleben beeinflussen können; ‘Wanderer’ sind dunkler, entwickeln sich schneller und das Gewicht ihrer Puppen ist niedriger als das der ‘Nicht-Wanderer’. 4. Eine der larvalen Farbvarianten zeigte eine Präferenz für einen von zwei angebotenen Hintergründen.
    Notizen: Abstract The dispersive behaviour of larvae of a polyphagous, wide-spread geometrid (Ectropis excursaria (Guenée)) was studied by examining responses to environmental and endogenous variables. It was found that differences in behaviour can be affected by environmental factors such as light, temperature, density, and plant background as well as some physiological and morphological features. The implications of these relationships are discussed as adaptative strategies to a varying environment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217535
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  • 6
    Digitale Medien
    Digitale Medien
    Foodplant selection and induction of feeding preference among host and non-host plants in larvae of the tobacco hornworm Manduca sexta (1984)
    Springer
    Entomologia experimentalis et applicata 35 (1984), S. 177-193 
    Details
    ISSN: 1570-7458
    Schlagwort(e): induction of feeding preference ; host plants ; non-host plants ; Manduca sexta ; Sphingidae ; Lepidoptera
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Vingt-deux espèces de plantes, dont 10 planteshôtes (Solanées), ont été testés comme plantes alimentaires pour des chenilles de ler stade de Manduca sexta. Sur cet ensemble, seulement 12 plantes (dont 9 plantes hôtes) induisaient la prise de nourriture et permettaient la croissance jusqu'au 5ème stade. La diversité des résultats suggère que les plantes pouvaient être classées en hôtes, non-hôtes acceptables et non-hôtes refusés. En utilisant le test du choix alimentaire préférentiel entre deux rondelles de feuilles, les chenilles néonates de ler stade ont préféré nettement les plantes-hôtes aux autres. Cette préférence initiale pour les plantes-hôtes était préservée quand les cheniles étaient élevées sur plantes-hôtes, mais devenait moins nette ou disparaissait pour des chenilles élevées sur d'autres plantes acceptées. Ainsi l'oligophagie ches M. sexta n'est pas induite, mais doit être héritée. Les chenilles néonates, aussi bien que celles de 5ème stade, présentent des préférences hiérarchisées parmi les plantes hôtes ou non. La seule frontiere nette observée était entre espèces de plantes acceptables ou non. Les hiérarchies préférentielles des chenilles du 5ème stade différaient légèrement lors-qu'elles avaient été élevées sur deux plantes-hôtes différentes. La différence essentielle était l'observation d'une préférence accrue pour l'espèce ayant servi à l'élevage, mais deux autres plantes-hôtes changaient aussi de position hiérarchique. La cause de ces changements de préférence a été approfondie, les chenilles étant élevées sur des feuilles de chaque espèce acceptable (hôte ou non). Leurs préférences alimentaires ont été définies en utilisant des combinaisons diverses (hôte x hôte, hôte x non-hôte acceptable, non-hôte acceptable x non-hôte acceptable). L'induction de la préférence alimentaires a été obtenue dans ces trois associations. Ceci montre que l'induction des choix alimentaires chez M. sexta n'est pas limitée aux plantes-hôtes. Le degré d'induction de la préférence alimentaire variait de très fort à indécelable; il dépendait de l'association examinée. La source de la variabilité de cette induction a été examinée en fonction de la relation entre la force de l'induction et les rapports taxonomiques des plantes associées. La relation obervée était inversée pour M. sexta. L'examen des données de la littérature ont révélé une relation du même type pour les autres espèces de Lépidoptères.
    Notizen: Abstract Ten host plant (Solanaceae) and twelve non-host plant species were tested as foodplants for first instar larvae of the tobacco hornworm, Manduca sexta. Only nine host and three non-host plant species elicited feeding and supported growth up to fifth instar. The range of acceptability suggested that plants be divided into hosts, acceptable non-hosts, and unacceptable non-hosts. Using the two-choice feeding preference test we found that the initial preference for hosts was preserved when larvae were reared on hosts, but was less strong or absent for larvae reared on acceptable non-hosts. Thus oligophagy in the tobacco hornworm is not induced, but must be inherited. Newly-hatched first instar larvae and fifth instar larvae showed a preference hierarchy among both hosts and non-hosts. Fifth instar larvae reared separately on two different host species showed slightly different preference hierarchies among hosts. The preference for the rearing plant was increased and also two other host species changed positions in hierarchies. Feeding preferences of larvae reared on hosts or acceptable non-hosts were determined using plant combinations of host vs. host, host vs. acceptable non-host, and acceptable non-host vs. acceptable non-host. Induction of feeding preference was found in all three of these categories. This shows that induction of feeding preference in the tobacco hornworm is not restricted to host plant species. The degree to which feeding preferences were induced ranged from very strong to undetectable and dependend on the plant species paired. The strength of induction in the tobacco hornworm was found to correlate inversely with taxonomic relatedness of the plant species paired. Analysis of induction data from the literature revealed a similar correlation for other lepidopteran species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217537
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  • 7
    Digitale Medien
    Digitale Medien
    Comparison of gravimetry and planimetry in determining dry matter budgets for three species of phytophagous lepidopteran larvae (1984)
    Springer
    Entomologia experimentalis et applicata 35 (1984), S. 255-261 
    Details
    ISSN: 1570-7458
    Schlagwort(e): method ; dry matter ; budget ; Lepidoptera ; phytophagous ; gravimetry ; area ; accuracy ; precision
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Les budgets en matière sèche consommée par des lépidoptères ont été comparés par les méthodes gravimétrique et planimétrique. La méthode gravimétrique est basée sur le rapport poids sec/poids frais de feuilles consommées par les chenilles. Avec la méthode planimétrique, la quantité d'aliment proposée aux chenilles est déterminée par les tracés de la surface des feuilles et le contenu de matière sèche par unité de surface des feuilles. La méthode de planimétrie permet l'utilisation de plus grands rameaux de feuilles et de cages d'élevage extérieures en gaze. Il n'y avait pas de différence appréciable dans les éléments du budget (croissance, ingestion et déjection), ni aucune différence dans la variabilité observée des données attribuable à la méthode utilisée. Cependant, la variabilité attendue d'après la précision des mesures avec la méthode gravimétrique est inférieure à celle de la méthode planimétrique. est inférieure à celle de la méthode planimétrique. Des éléments expérimentaux, inhérents à la méthode gravimétrique, introduisent une variabilité dans les mesures que l'on n'a pas avec la méthode planimétrique. 30–60% de la variabilité dans la consommation ont été attribués à des paramètres internes à la chenille, même quand elles provenaient toutes de la même ooplaque.
    Notizen: Abstract Gravimetric and a combination areal-gravimetric methods for determining dry matter budgets for leaf eating Lepidoptera were compared. The gravimetric method is based on dry weight/live weight ratios of the leaves fed to the larvae. In the areal-gravimetric method, the quantity of food offered to the larvae is determined from the area of leaf tracings and the dry matter content per unit area of the leaves. The areal-gravimetric method permits the use of larger leaf sprays and an open, gauze enclosed rearing chamber. There were no consistent differences in budget factors (growth, ingestion or egestion), nor were there any differences in the observed variability of the data attributable to the method used. However, the expected variability based on instrument precision for the gravimetric method is less than for the areal-gravimetric method. Experimental factors inherent in the gravimetric method introduce variability to the measurements that are not present in the areal method. Thirty to 60% of the variability in budget factors was attributed to intrinsic properties of the larvae, even though the larvae were taken from the same egg masses.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00369141
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  • 8
    Digitale Medien
    Digitale Medien
    Immunocytochemical and electrophoretic studies on the localization of the major haemolymph proteins (ceratitins) inCeratitis capitata during development (1984)
    Springer
    Development genes and evolution 194 (1984), S. 37-43 
    Details
    ISSN: 1432-041X
    Schlagwort(e): Major haemolymph proteins ; Development ; Cuticle ; Immunocytochemistry ; Ceratitis capitata
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The developmental profile of the major haemolymph proteins (ceratitins) inCeratitis capitata was studied. Ceratitin concentration in the haemolymph decreases dramatically during the last days of pupal life, while the amounts of ceratitins in whole organism extracts remain unchanged. By electrophoretic, immunological and immunofluorescence techniques it was revealed that ceratitins are reabsorbed by the fat body and a fraction of them is deposited in the cuticle. The possible role of ceratitins is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00848952
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  • 9
    Digitale Medien
    Digitale Medien
    Length and shape of enamel crystals (1984)
    Springer
    Calcified tissue international 36 (1984), S. 550-555 
    Details
    ISSN: 1432-0827
    Schlagwort(e): Enamel crystals ; Length ; Shape ; Apatite ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Summary An original method for fractionating and preparing isolated crystals of homogeneous size was developed. It was demonstrated that enamel apatite crystals are at least 100 µm long. The flexibility of the very long crystallites was demonstrated. Crystal curvatures, accounting for the irregular course of the prisms through the enamel thickness, were visualized and measured. It was shown that in the deep forming enamel layer, lateral branches may grow out of the crystals and crystal fusing often occurs, inducing the crystallites to assume pyramidal shapes with their wide bases pointing toward the dentino-enamel junction and one or two tops toward Tomes' processes. During the maturation process, the two tops of the still immature crystals also fuse so that the mature crystals acquire a rodlike aspect, with parallel faces and steplike graduations along thec axis, allowing a close contact between the crystals. These results support the hypothesis that the crystallites would be continuous from the dentino-enamel junction to the surface.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02405364
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  • 10
    Digitale Medien
    Digitale Medien
    Localization of phytohemagglutinin in the embryonic axis of Phaseolus vulgaris with ultra-thin cryosections embedded in plastic after indirect immunolabeling (1984)
    Springer
    Planta 162 (1984), S. 548-555 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Immunocytochemistry ; Lectin (localization) ; Phaseolus (lectin) ; Phytohemagglutinin ; Seed (lectin)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have examined the properties and subcellular localization of phytohemagglutinin (PHA), the major lectin of the common bean (Phaseolus vulgaris.), in the axis cells of nearly mature and imbibed mature seeds. On a protein basis the axis contained about 15% as much PHA as the cotyledons. Localization of PHA was done with an indirect immunolabeling method (rabbit antibodies against PHA, followed by colloidal gold particles coated with goat antibodies against rabbit immunoglobulins) on ultra-thin cryosections which were embedded in plastic on the grids after the immunolabeling procedure. The embedding greatly improved the visualization of the subcellular structures. The small (4 nm) collodial gold particles, localized with the electron microscope, were found exclusively over small vacuoles or protein bodies in all the cell types examined (cortical parenchyma cells, vascular-bundle cells, epidermal cells). The matrix of these vacuoles-protein bodies appears considerably less dense than that of the protein bodies in the cotyledons, but the results confirm that in all parts of the embryo PHA is localized in similar structures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00399921
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  • 11
    Digitale Medien
    Digitale Medien
    Isolation and characterization of a new coccoid methanogen, Methanogenium tatii spec. nov. from a solfataric field on Mount Tatio (1984)
    Springer
    Archives of microbiology 137 (1984), S. 308-315 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Methanogenium tatii ; Ultrastructure ; Physiology ; Glycoproteins ; DNA-DNA Homology ; Taxonomy ; Archaebacteria
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A new coccoid methanogen, Methanogenium tatii, was isolated and characterized. The mesophilic isolate can grow on and produce methane from H2:CO2 and formate. For growth acetate is strictly required. The cell shape, the G+C content of 54 mol% and DNA-DNA homology data suggest it to be a Methanogenium species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00410727
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  • 12
    Digitale Medien
    Digitale Medien
    Fine structure of the knobby spore type of Streptomyces torulosus (1984)
    Springer
    Archives of microbiology 138 (1984), S. 229-232 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Actinomycetes ; Streptomyces torulosus ; Morphology ; Ultrastructure ; Verrucate spores ; Knobby ornamentation ; Sheath
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The type strain of Streptomyces torulosus Lyons and Pridham (1971) was studied by scanning- and transmission electron microscope. Spore chains were formed in spirals by aerial mycelium. The spores were connected by nozzles in which small channels could be observed. The knobby ornamentations of the spores arised on a thin fibrous sheath, enveloping the spore chains. These irregular blunt projections, called knobs, had varying diameters of 100 to 250 nm. The base of the knob, consisting of globose to flattened electron dense material, was sitting directly on the sheath. It was covered by several small vesicles of the same material. Each hollow vesicle beared a thin bowlshaped shell of electron transparent material. In general, the cupular bowls and their supporting vesicles became easily depressed on their base, but not detached from the surface of the spores. This type of knobby spore ornamentation was suggested to be designated as a verrucate spore type.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00402126
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  • 13
    Digitale Medien
    Digitale Medien
    Membrane-bound nitrite oxidoreductase of Nitrobacter: evidence for a nitrate reductase system (1984)
    Springer
    Archives of microbiology 140 (1984), S. 153-158 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Nitrobacter hamburgensis ; Nitrite oxidoreductase ; Nitrate reductase ; Molybdenum iron-sulfur protein ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Nitrite oxidoreductase, the essential enzyme complex of nitrite oxidizing membranes, was isolated from cells of the nitrifying bacterium Nitrobacter hamburgensis. The enzyme system was solubilized and purified in the presence of 0.25% sodium deoxycholate. Nitrite oxidoreductase oxidized nitrite to nitrate in the presence of ferricyanide. The pH optimum was 8.0, and the apparent K m value for nitrite amounted to 3.6 mM. With reduced methyl-and benzylviologen nitrite oxidoreductase exhibited nitrate reductase activity with an apparent K m value of 0.9 mM for nitrate. NADH was also a suitable electron donor for nitrate reduction. The pH optimum was 7.0. Treatment with SDS resulted in the dissociation into 3 subunits of 116,000, 65,000 and 32,000. The enzyme complex contained iron, molydbenum, sulfur and copper. A c-type cytochrome was present. Isolated nitrite oxidoreductase is a particle of 95±30 Å in diameter.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00454918
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  • 14
    Digitale Medien
    Digitale Medien
    Attraction of male spruce budworm moths,Choristoneura fumiferana (Clemens), to pheromone-baited traps in small-tree thinnings (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 125-133 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Spruce budworm ; Choristoneura fumiferana ; Lepidoptera ; Tortricidae ; sex pheromone ; small-tree thinnings ; temperature ; precipitation ; wind ; attraction distance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Mean catches of spruce budworm,Choristoneura fumiferana (Clemens), moths were not significantly different among four small-tree thinning treatments of young spruce-fir-hemlock regeneration. Significant inverse relationships were found between trap catches and distances to nearby spruce-fir-hemlock overstory. Prevailing wind directions indicated that moths were attracted anemotactically to upwind pheromone sources. No definite trends were detected between catches and temperature or precipitation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00987649
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  • 15
    Digitale Medien
    Digitale Medien
    Relative kairomonal activities of 2-acylcyclohexane-1,3-diones in eliciting oviposition behavior from parasiteNemeritis canescens (Grav.) (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1597-1601 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Kairomone ; 2-acylcyclohexane-1,3-diones ; ovipositionEphestia kuehniella Zeller [syn.Anagasta kuehniella (Zeller)] ; Lepidoptera ; Pyralidae ; Nemeritis canenscens (Grav.) [syn.Venturia canescens (Grav.)] ; Hymenoptera ; Ichneumonidae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The relative activities of sixteen 2-acylcyclohexane-1,3-diones from the larval mandibular glands ofEphestia (=Anagasta) kuehniella Zeller in causing the parasiteNemeritis (=Venturia) canescens (Grav.) to make oviposition movements are reported.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00988427
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  • 16
    Digitale Medien
    Digitale Medien
    Potential for evolution of resistance to pheromones: Interindividual and interpopulational variation in chemical communication system of pink bollworm moth (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1551-1565 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Resistance ; mating disruption ; sex pheromone ; (Z,Z)-7 ; 11-hexadecadienyl acetate ; (Z,E)-7 ; 11-hexadecadienyl acetate ; Lepidoptera ; Gelechiidae ; pink bollworm ; Pectinophora gossypiella ; cotton ; pheromone collection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract After an extensive examination of the release rates and blend ratios of pheromonal components emitted by field-collected femalePectinophora gossypiella (Saunders), we find no evidence of resistance to pheromones applied to cotton fields to disrupt mating. Females from fields with 3–5 years of exposure to disruptant pheromones as well as those from fields with only minimal exposure to disruptant pheromones emitted (Z,Z)-7,11-hexadecadienyl acetate at a rate of ca. 0.1 ng/min and (Z,E)7,11-hexadecadienyl acetate at ca. 0.06 ng/min. The ratio of pheromonal components was much less variable than the measured emission rate and was centered about a 61:39Z, Z to Z,E ratio. In contrast to the blend ratio emitted by females, the composition of the pheromonal blend used in monitoring populations and disrupting mating is centered about 50:50 Z,Z to Z.E. In general there was a remarkable consistency in the release rate and blend ratio among populations of females throughout southern California and those from a laboratory colony. It would appear that, although resistance to theP. gossypiella pheromone is still a very real possibility when it is used heavily in pest management as a mating disruptant, there are current agricultural practices and conditions which would hinder its development.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00988424
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  • 17
    Digitale Medien
    Digitale Medien
    Structure-activity relationships between stimulus molecule and response of a pheromone receptor cell in turnip moth,Agrotis segetum (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 1661-1675 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Structure-activity relationships ; single-cell recordings ; turnip moth ; Agrotis segetum ; Lepidoptera ; Noctuidae ; molecular shape ; dipole moments
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The response of an antennal receptor cell of the turnip moth,Agrotis segetum, was recorded during stimulation with a series of (Z)-7-dodecenyl acetate analogs with structural variations of the acetate group. The investigated receptor cell is known to be highly selective to (Z)-7-dodecenyl acetate. All parts of the acetate group were found to be of great importance for full biological activity. The results indicate very strict requirements on the shape of the polar functional group, as well as on its electron distribution for a successful interaction with the antennal receptor cell.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00987353
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  • 18
    Digitale Medien
    Digitale Medien
    Plant-determined variation in the cardenolide content, thin-layer chromatography profiles, and emetic potency of monarch butterflies,Danaus plexippus L. Reared on milkweed plants in California: 2.Asclepias speciosa (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 601-639 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Danaus plexippus ; Lepidoptera ; Danaidae ; monarch butterflies ; Asdepias speciosa ; Asclepiadaceae ; milkweeds ; ecological chemistry ; plant-insect interactions ; chemical ecology ; chemical defense ; coevolution ; thin-layer chromatography ; cardenolide fingerprints ; cardenolides ; cardiac glycosides ; desglucosyrioside ; labriformin ; labriformidin ; syriogenin ; uzarigenin ; emetic potency ; emesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The pattern of variation in gross cardenolide concentration of 111Asclepias speciosa plants collected in six different areas of California is a positively skewed distribution which ranges from 19 to 344 μg of cardenolide per 0.1 g dry weight with a mean of 90 μg per 0.1 g. Butterflies reared individually on these plants in their native habitats ranged from 41 to 547 μg of cardenolide per 0.1 g dry weight with a mean of 179 μg. Total cardenolide per butterfly ranged from 54 to 1279 μg with a mean of 319 μg. Differences in concentrations and total cardenolide contents in the butterflies from the six geographic areas appeared minor, and there were no differences between the males and the females, although the males did weigh significantly more than females. The uptake of cardenolide by the butterflies was found to be a logarithmic function of the plant concentration. This results in regulation: larvae which feed on low-concentration plants produce butterflies with increased cardenolide concentrations relative to those of the plants, and those which feed on high-concentration plants produce butterflies with decreased concentrations. No evidence was adduced that high concentrations of cardenolides in the plants affected the fitness of the butterflies. The mean emetic potencies of the powdered plant and butterfly material were 5.62 and 5.25 blue jay emetic dose fifty units per milligram of cardenolide and the number of ED50 units per butterfly ranged from 0.28 to 6.7 with a mean of 1.67. Monarchs reared onA. speciosa, on average, are only about one tenth as emetic as those reared onA. eriocarpa. UnlikeA. eriocarpa which is limited to California,A. speciosa ranges from California to the Great Plains and is replaced eastwards byA. syriaca L. These two latter milkweed species appear to have a similar array of chemically identical cardenolides, and therefore both must produce butterflies of relatively low emetic potency to birds, with important ecological implications. About 80% of the lower emetic potency of monarchs reared on A. speciosa compared to those reared onA. eriocarpa appears attributable to the higher polarity of the cardenolides inA. speciosa. Thin-layer Chromatographie separation of the cardenolides in two different solvent systems showed that there are 23 cardenolides in theA. speciosa plants of which 20 are stored by the butterflies. There were no differences in the cardenolide spot patterns due either to geographic origin or the sex of the butterflies. As when reared onA. eriocarpa, the butterflies did not store the plant cardenolides withR f values greater than digitoxigenin. However, metabolic transformation of the cardenolides by the larvae appeared minor in comparison to when they were reared onA. eriocarpa. AlthoughA. eriocarpa andA. speciosa contain similar numbers of cardenolides and both contain desglucosyrioside, the cardenolides ofA. speciosa overall are more polar. ThusA. speciosa has no or only small amounts of the nonpolar labriformin and labriformidin, whereas both occur in high concentrations inA. eriocarpa. A. speciosa plants and butterflies also contain uzarigen, syriogenin, and possibly other polar cardenolides withR f values lower than digitoxin. The cardenolide concentration in the leaves is not only considerably less than inA. eriocarpa, but the latex has little to immeasurable cardenolide, whereas that ofA. eriocarpa has very high concentrations of several cardenolides. Quantitative analysis ofR f values of the cardenolide spots, their intensities, and their probabilities of occurrence in the chloroform-methanol-formamide TLC system produced a cardenolide fingerprint pattern very different from that previously established for monarchs reared onA. eriocarpa. This dispels recently published skepticism about the predictibility of chemical fingerprints based upon ingested secondary plant chemicals.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00994224
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  • 19
    Digitale Medien
    Digitale Medien
    Metabolism of uscharidin, a milkweed cardenolide, by tissue homogenates of monarch butterfly larvae,Danaus plexippus L. (1984)
    Springer
    Journal of chemical ecology 10 (1984), S. 945-956 
    Details
    ISSN: 1573-1561
    Schlagwort(e): Cardenolide ; uscharidin ; metabolism ; monarch butterfly ; Danaus plexippus ; Lepidoptera ; Danaidae ; milkweed ; Asclepias ; N-demethylation ; mixed function oxidase ; monooxygenase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Midgut and fat body homogenates of monarch butterfly larvae,Danaus plexippus L. (Lepidoptera:Danaidae), were examined for microsomal monooxygenase activity usingp-chloro-N-methylanilineN-demethylation and for the ability to metabolize a milkweed (Asclepias spp.) cardenolide (C23 steroid glycoside), uscharidin. All homogenates tested had bothN-demethylation and uscharidin biotransformation activities. Both transformations required NADPH. The monooxygenase inhibitors sesamex, SKF525A, and carbon monoxide inhibitedN-demethylation but not uscharidin biotransformation. Subsequent subcellular fractionation revealed the uscharidin biotransformation occurs in the soluble fraction and not the microsomal fraction, whileN-demethylation occurs in the microsomal fraction and not the soluble fraction. The larval NADPH-dependent microsomal monooxygenase apparently is not involved in the metabolism of uscharidin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00987975
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  • 20
    Digitale Medien
    Digitale Medien
    Ultrastructure of gill epithelial cells of Diopatra neapolitana (Annelida, Polychaeta) (1984)
    Springer
    Zoomorphology 104 (1984), S. 304-309 
    Details
    ISSN: 1432-234X
    Schlagwort(e): Ultrastructure ; Gills ; Epithelial cells ; Polychaeta
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The ultrastructure of gill epidermal cells of Diopatra neapolitana and their relationship with blood spaces are described. The existence of a basal infolding complex, related to the blood spaces, is also reported. A possible involvement of these cells in osmoregulation and ion interchange, apart from their well-known role in respiration, is suggested.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00312012
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  • 21
    Digitale Medien
    Digitale Medien
    Unequal distribution of plastids during generative cell formation in Impatiens (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 305-309 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Impatiens ; Microspore mitosis ; Plastid distribution ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary This paper describes the unequal distribution of plastids in the developing microspores of Impatiens walleriana and Impatiens glandulifera which leads to the exclusion of plastids from the generative cell. During the development from young microspore to the onset of mitosis a change in the organization of the cytoplasm and distribution of organelles is gradually established. This includes the formation of vacuoles at the poles of the elongate-shaped microspores, the movement of the nucleus to a position near the microspore wall in the central part of the cell, and the accumulation of the plastids to a position near the wall at the opposite side of the cell. In Impatiens walleriana, the accumulated plastids are separated from each other by ER cisterns, and some mitochondria are also accumulated. In both Impatiens species, the portion of the microspore in which the generative cell will be formed is completely devoid of plastids at the time mitosis starts.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00267882
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  • 22
    Digitale Medien
    Digitale Medien
    Degenerative regression of the digestive tract in the colonial ascidian Botryllus schlossen (Pallas) (1984)
    Springer
    Cell & tissue research 235 (1984), S. 309-318 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Ascidian ; Gut ; Cell involution ; Ultrastructure ; Phagocytes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Degenerative changes in the digestive tract of zooids of Botryllus schlosseri were studied by light and electron microscopy. Three main processes occurred in the tissues: contraction, involution and phagocytosis. The contraction of epidermis and peribranchial epithelium in which cytoplasmic microfilaments probably participate, seemed to have a special role in compressing the underlying organs. During contraction most of the body cavities collapsed, the branchial walls disintegrated and the fragments were rapidly taken up by large phagocytes. The gut epithelium retained its apparent continuity longer, though isolated phagocytes infiltrated it to eliminate single cells. Cell degeneration came about chiefly either through swelling and lysis of cells or through loss of water and condensation of cytoplasm and nucleus. The fate of all regressed tissues was to be engulfed and digested by wandering phagocytes. However, it was also observed that numerous cells of different epithelia could act as fixed phagocytes by engulfing cell debris and entire cells into heterophagic vacuoles.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217855
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  • 23
    Digitale Medien
    Digitale Medien
    The pig blastocyst: Its ultrastructure and the uptake of protein macromolecules (1984)
    Springer
    Cell & tissue research 235 (1984), S. 347-356 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Blastocyst ; Ultrastructure ; Pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Between days 8 and 11 of pregnancy spherical blastocysts from 0.3 to 10 mm in diameter were flushed from the uterine horns of Dutch Landrace pigs. A description of their ultrastructure is given, and the uptake of horseradish peroxidase and ferritin is demonstrated. The ultrastructure of the trophoblast was similar at all ages studied. The trophoblast which has many apical microvilli is able to take up and digest the macromolecules which were offered in the in vitro incubation medium. The hypoblast consists of flattened cells. In blastocysts 2 mm and larger, compact cells bearing microvilli are found below the embryoblast. Cell organelles indicating protein synthesis are found within hypoblast cells of such blastocysts. In the embryoblast, local concentrations of cell organelles are visible, indicating that differentiation has started. After the disappearance of Rauber's layer, which takes place when the blastocyst reaches a diameter of about 2 mm, superficial embryoblast cells develop short microvilli. The cells do not absorb ferritin or peroxidase but are dependent on the trophoblast for their food requirements. All cell layers in the blastocyst contain mitochondria that have characteristics of those found in steroidproducing cells. The significance of the uptake and digestion of macromolecules by trophoblast cells, the synthesis of protein by hypoblast cells and the possible synthesis of steroids is discussed with respect to the relationship between the cell layers of the blastocyst and in the context of conceptomaternal relationships.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217859
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  • 24
    Digitale Medien
    Digitale Medien
    Changes in human skeletal muscle induced by long-term eccentric exercise (1984)
    Springer
    Cell & tissue research 236 (1984), S. 365-372 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Skeletal muscles ; Myofibrils ; Ultrastructure ; Exertion ; Man
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The fine structure of muscle fibres from m. vastus lateralis of nine healthy males (mean age 26 years) was investigated. Four individuals constituted non-exercised controls while five subjects participated in a two-months eccentric muscular training program. Specimens from the controls showed a well-preserved, regular myofibrillar band pattern while changes in the myofibrillar architecture were constantly found in specimens taken after the training program. These changes consisted of Z-band alterations, Z-bands being out of register, extra sarcomeres, Z-band extensions and bisected Z-bands. Between the separated Z-band halves, thin and thick myofilaments as well as abundant glycogen particles and/or ribosomes, were observed. Type-2 (fast-twitch) fibres were predominantly affected. Contrary to the controls the trained individuals constantly showed a greater variation in sarcomere lengths in Type-2 fibres than in Type-1 fibres. It is concluded that muscular work of high tension can induce fine-structural alterations. When repeated over a long period of time, extreme tension demands seem to initiate reorganization in the muscle fibres, predominantly in the, ultrastructurally defined, Type-2 fibres. This adaptation probably results in a better stretchability of the muscle fibres, reduces the risk for mechanical damage and brings about an optimal overlap between actin and myosin filaments.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00214240
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  • 25
    Digitale Medien
    Digitale Medien
    Ultrastructure and morphometry of the stomach muscle of Amphiuma tridactylum (1984)
    Springer
    Cell & tissue research 236 (1984), S. 393-397 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Smooth muscle ; Salamander, Amphiuma ; Ultrastructure ; Stereology ; Volume: surface area ratio
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary An ultrastructural and stereological examination was performed on stomach smooth muscle of the salamander Amphiuma. This tissue has very large cells, ranging up to 12×1500 μm when relaxed. The extracellular space is 31% of the tissue volume, and the tissue contains 84.6% water. These values are similar to those of other amphibian and mammalian gastrointestinal smooth muscle. The cells possess the usual smooth muscle organelles. Thick, thin and intermediate filaments are present, along with membrane-associated and cytoplasmic dense regions. There is a well-developed sarcoplasmic reticulum and many microtubules. Caveolae are found in rows along the cellular surface; the caveolae increase the cellular surface area by about 70%. The ratio mean volume: surface area of the cells is 1.26 μm. This tissue appears to be typical of gastrointestinal smooth muscle, with the exception of the very large size of the cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00214243
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  • 26
    Digitale Medien
    Digitale Medien
    Reproduction and sexual development in a freshwater gastrotrich (1984)
    Springer
    Cell & tissue research 236 (1984), S. 619-628 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Gastrotricha, freshwater ; Sperm, reduced ; Ultrastructure ; Spermatogenesis ; Temperature
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Spermatogenesis and spermiogenesis in Lepidodermella squammata are confined to the postparthenogenic phase of the life cycle and coincide with developmental changes in the bilateral female gonads. Male stages are bilateral but asynchronous, in the lateral abdomen anterior to the female gonads. Maximum observed sperm production is two packets per side, or 64 sperm. Sperm formation occurs more rapidly at 27° C than at 20° C (p〈0.001), requiring as little as 1 day. Two spermatogonial mitotic divisions produce a clone of four primary spermatocytes connected by bridges (stage 1). Centrioles are absent. Development occurs within a cyst. Meiotic divisions produce 16 spermatids (stage 2), each containing a dense, elongate, tapered nucleus. Cytoplasmic membranes enclose one end of the nuclear rod, excluding all other organelles. Completion of this process results in stage 3, a packet of 16 sperm associated with one dense sphere, a modified ‘residual body’ containing cytoplasmic debris. The residual body then disappears, leaving the sperm packet of stage 4. Each mature sperm is a dense nuclear rod with surrounding membranes, lacking acrosome, mitochondrion, centrioles, and flagellum. Function of sperm has not been demonstrated. The spermatozoa are of a reduced type not previously described.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217231
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  • 27
    Digitale Medien
    Digitale Medien
    Reproduction and sexual development in a freshwater gastrotrich (1984)
    Springer
    Cell & tissue research 236 (1984), S. 629-636 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Oocytes, primary ; Gastrotricha, freshwater ; Ultrastructure ; Synaptonemal complex ; X-body
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Six small cells are present in each of the bilateral gonads of parthenogenically reproductive Lepidodermella squammata. Early in the extended postparthenogenic phase of the life history, these cells undergo limited proliferation followed by differentiation. Primary oocytes of three types are present 0.3 days after deposition of the final parthenogenic egg: small oocytes with presynaptic nuclei; intermediate oocytes with nuclei containing synaptonemal complexes; and larger oocytes with a germinal vesicle. Oocytes persist without further development at least until day four of the postparthenogenic phase. Older isolated animals may contain and even deposit an enlarged egg, but successful progeny does not result. Oocytes are located at the anterior pole of each of the bilateral gonads, adjacent to developing male tissues producing sperm. More posterior cells in the gonad are initially undifferentated in the postparthenogenic phase. Dorsal and central cells first show specialization for secretory activity, and by day four contain peripheral layers of RER and central accumulations of polymorphic secretion droplets. The posterior and ventral cells produce secretion droplets that aggregate into an enlarging bilobed structure called the X-body. Two or three cells in each gonad contribute secretions to the X-body, which is intracellular in a secondary syncytium formed by the contributing cells. Functions for the postparthenogenic gametes and for the X-body are not yet demonstrated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217232
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  • 28
    Digitale Medien
    Digitale Medien
    Arachnoid mater of the bullfrog, Rana catesbeiana (1984)
    Springer
    Cell & tissue research 236 (1984), S. 693-697 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Intermediate filaments ; Microtubules ; Caveolae ; Bullfrog ; Arachnoid mater ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In the bullfrog, the meninges surrounding the central nervous system include an arachnoid mater that contains layers of cells with abundant intermediate filaments (IFs) having unique organizational characteristics. This membrane contains an inner lamina of cells that resemble fibroblasts and an outer lamina of flattened cells that are almost filled with IFs. The IFs of the outer arachnoid are arranged in compact, arching bundles that lie parallel to the outer surface of the central nervous system. Thus, sections cut tangentially to the membrane reveal bending of filament bundles, whereas transverse sections do not. In some cells bordering the subdural space, bundles of filaments are organized into highly-ordered spiral arrays. Attachments to the numerous desmosomes and, apparently, to the nuclear envelope suggest anchoring of cytoplasmic structures by the IF system. Microtubules occur primarily near the plasma membrane and the nucleus. Numerous caveolae also are associated with the plasma membrane. The unusual abundance, organization, and cytoplasmic relations of IFs in the bullfrog arachnoid suggest that this membrane may serve as an important model for study of fundamental cytoskeletal relations and function.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217240
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  • 29
    Digitale Medien
    Digitale Medien
    Fine-structural heterogeneity and morphologic changes in rat pituitary prolactin cells after estrogen and testosterone treatment (1984)
    Springer
    Cell & tissue research 237 (1984), S. 195-202 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pituitary ; Prolactin cells ; Estrogen ; Heterogeneity ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary This study was conducted to determine the functional and/or developmental relationships among three heterogeneous types of prolactin cells (I, II and III) in rats. Rats were injected subcutaneously daily with estradiol or testosterone propionate on days 10–20 after birth. Estradiol increased the proportion of cell types II and III, increased serum PRL levels 12-fold in males and 15-fold in females, and increased pituitary levels of prolactin 12-fold in males and 5-fold in females. Testosterone mainly increased the proportion of the Type-II cells, decreased serum levels of prolactin in males only, and did not change pituitary levels of prolactin. In a second experiment, treatment of rats with nafoxidine for five days after E2 treatment (days 10–20 after birth) increased the proportion of Type-I cells and decreased the proportion of Type-III cells and decreased serum and pituitary levels of prolactin by 50% in females and by 15 and 45% in males. In a third experiment utilizing adult male rats, estradiol and testosterone were found to modulate the relative ratios of the different types of PRL cells as they did in immature animals. The data taken as a whole suggest the possibility of an estrogen-stimulated conversion of one cell type to another, which may be a reflection of prolactin secretory activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217136
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  • 30
    Digitale Medien
    Digitale Medien
    Glial cells in the pineal gland of mice and rats (1984)
    Springer
    Cell & tissue research 237 (1984), S. 245-252 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pineal organ ; Interstitial cells ; Astrocytes ; Immunocytochemistry ; Rat ; Mouse
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Antigenic markers characteristic of astrocytes and their differentiative states (i.e., glial fibrillary acidic protein (GFAP), vimentin, and M1 and C1 antigens) were investigated in the pineal gland of mouse and rat using double immunolabeling techniques. In both species the socalled interstitial cells as characterized by TEM were shown to be astrocytes, since they expressed vimentin, but neither fibronectin (a marker for fibroblasts and endothelial cells) nor the neuron-specific L1 antigen or tetanus toxin receptors. Subpopulations of vimentin-positive pineal astrocytes were also GFAP- and C1- antigen-positive. M1- antigenpositive cells were not detected. It is concluded that a considerable proportion of interstitial cells in the pineal gland of rat and mouse are immature astrocytes which, in contrast to other parts of the central nervous system, persist into adulthood.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217142
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  • 31
    Digitale Medien
    Digitale Medien
    Ultrastructure and innervation of the swimbladder of Tetractenos glaber (Tetraodontidae) (1984)
    Springer
    Cell & tissue research 237 (1984), S. 277-284 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Swimbladder ; Teleost ; Cholinergic nerves ; Adrenergic nerves ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The general structure, ultrastructure and innervation of the swimbladder of the smooth toadfish, Tetractenos glaber, were examined with light-microscopic, fluorescence-histochemical, and transmission electron-microscopic techniques. The structure of the swimbladder is similar to that of other euphysoclists. Fluorescence histochemistry showed adrenergic fibres in both the secretory and resorptive areas of the swimbladder. Transmission electron microscopy revealed two morphologically distinct axon profiles type-I profiles containing many small, flattened vesicles; type-II profiles containing both large, granular vesicles and rounded, small clear vesicles in varying proportions. The gas-gland cells and surrounding muscularis mucosae are innervated by both type-I and type-II fibres. Type-I fibres also innervate pre-rete arteries. The rete- and gas-gland capillaries do not appear to be innervated. Arteries running to the resorptive area are innervated by type-I fibres. Both type-I and type-II profiles make contact with the muscularis mucosae in the resorptive area. Only type-I fibres innervate the radial dilator muscle in the oval sphincter region, whereas only type II fibres innervate the circular muscle of the oval sphincter. Type-I fibres took up α-methyl-noradrenaline, and could not be found after pre-treatment with 6-hydroxydopamine. They are, therefore, assumed to be adrenergic. Type-II fibres were tentatively identified, by exclusion, as cholinergic.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217146
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  • 32
    Digitale Medien
    Digitale Medien
    Comparative immunocytochemical study of the subcommissural organ (1984)
    Springer
    Cell & tissue research 237 (1984), S. 427-441 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Subcommissural organ ; Reissner's fiber ; Ependyma ; Secretory process ; Comparative analysis ; Immunocytochemistry ; Vertebrates
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The subcommissural organs (SCO) of 76 specimens belonging to 25 vertebrate species (amphibians, reptiles, birds, mammals) were studied by use of the immunoperoxidase procedure. The primary antiserum was obtained by immunizing rabbits with bovine Reissner's fiber (RF) extracted in a medium containing EDTA, DTT and urea. Antiserum against an aqueous extract of RF was also produced. The presence of immunoreactive material in cell processes and endings was regarded as an indication of a possible route of passage. Special attention was paid to the relative development of the ventricular, leptomeningeal and vascular pathways established by immunoreactive structures. The SCO of submammalian species is characterized by (i) a conspicuous leptomeningeal connection established by ependymal cells, (ii) scarce or missing hypendymal cells, and (iii) a population of ependymal cells establishing close spatial contacts with blood vessels. The SCO of most mammalian species displays the following features: (i) ependymal cells lacking immunoreactive long basal processes, (ii) hypendymal secretory cells occurring either in a scattered arrangement or forming clusters, (iii) an occasional leptomeningeal connection provided by hypendymal cells, and (iv) in certain species numerous contacts of secretory cells with blood vessels. In the hedgehog immunoreactive material was missing in the ependymal formation of the SCO, but present in hypendymal cells and in the choroid plexuses. The SCO of several species of New-and Old-World monkeys displayed immunoreactive material, whereas that of anthropoid apes (chimpanzee, orangutan) and man was completely negative with the antisera used.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228427
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  • 33
    Digitale Medien
    Digitale Medien
    Spatial and structural interrelationships between secretory cells of the subcommissural organ and blood vessels (1984)
    Springer
    Cell & tissue research 237 (1984), S. 443-449 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Subcommissural organ ; Ependyma ; Comparative aspects ; Immunocytochemistry ; Secretory process ; Blood vessels
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In 76 specimens (amphibians, reptilians, mammals) belonging to 25 different vertebrate species, the region of the subcommissural organ (SCO) was investigated with the use of a primary antiserum raised against an extract of bovine Reissner's fiber+the immunoperoxidase procedure according to Sternberger et al. (1970). In the SCO of a toad (Bufo arenarum) and several species of reptiles (lacertilians, ophidians, crocodilians), the ependymal cells were the only type of secretory cell displaying vascular contacts, whereas in mammals ependymal and hypendymal cells established intimate spatial contacts with blood vessels. In Bufo arenarum, but especially in the reptilian species examined, the ependymo-vascular relationship was exerted by a population of ependymal cells having a rather constant location within the SCO and projecting to capillaries that showed a remarkably constant pattern of anatomical distribution. In the SCO of mammals the modality and degree of the structural relationships between secretory cells and blood vessels varied greatly from species to species. In the SCO of the armadillo and dog the secretory tissue was organized as a thick, highly vascularized layer with most of the cells oriented toward the capillaries. A rather opposite situation was found in the SCO of New-and Old-World monkeys, where vascular contacts were restricted to a few ependymal cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228428
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  • 34
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of α-melanocyte-stimulating hormone in the brain of the lizard, Lacerta muralis (1984)
    Springer
    Cell & tissue research 237 (1984), S. 521-524 
    Details
    ISSN: 1432-0878
    Schlagwort(e): α-Melanocyte-stimulating hormone ; α-MSH-like peptide ; Immunocytochemistry ; Hypothalamus ; Lizard (Lacerta muralis)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The distribution of α-melanocyte-stimulating hormone (α-MSH) was studied in the brain of the lizard Lacerta muralis by means of immunocytochemical staining methods. α-MSH-like containing cells were found in the ventro-lateral preoptic area and the paraventricular and supraoptic nuclei. Some scattered cells staining for α-MSH were also detected in the mesencephalo-diencephalic boundary region, while numerous α-MSH-like nerve fibres were localized in the medial eminence. No reaction was observed after the use of antiserum preabsorbed with synthetic antigen. These findings suggest that an α-MSH-like peptidergic system could possibly be involved in the hypothalamo-hypophysial regulation and/or play a role as neurotransmitter in this animal.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228436
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  • 35
    Digitale Medien
    Digitale Medien
    Fine structure of regenerating scales and their associated cells in the cichlid Hemichromis bimaculatus (Gill) (1984)
    Springer
    Cell & tissue research 237 (1984), S. 537-547 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Scale ; Regeneration ; Ultrastructure ; Cichlid ; Hemichromis bimaculatus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Scale regeneration has been studied in Hemichromis bimaculatus. The removed scale, which serves as a control, is covered by its surrounding scleroblasts as can be seen with scanning electron microscopy. Subsequently, during regeneration, a population of scleroblasts arises in the empty dermal pocket as shown with transmission electron microscopy. At first, an elongated papilla of regeneration forms, probably from the differentiation of dermal fibroblasts. A scale anlage composed of the osseous layer appears in the middle of the papilla, which becomes a regenerating bag. All the surrounding large scleroblasts are involved in scale formation, although later three populations of scleroblasts specialize according to their location around the scale. Superficial scleroblasts flatten when the final thickness of the osseous layer of the scale is attained; the deep scleroblasts are responsible for the formation of the basal plate whereas marginal scleroblasts increase the diameter of the osseous layer of the scale. During scale regeneration, scleroblasts are more numerous and larger than during scale ontogenesis. In particular, deep scleroblasts form a columnar epithelium when the basal plate is laid down, a feature which is not found during scale ontogenesis. Moreover, the regenerated basal plate exhibits an orthogonal “plywood” arrangement that is never seen in the embryonic scale where the “plywood” is of the intermediate type.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228438
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  • 36
    Digitale Medien
    Digitale Medien
    Ultrastructure of the epididymis of the tammar, Macropus eugenii, and its relationship to sperm maturation (1984)
    Springer
    Cell & tissue research 237 (1984), S. 525-535 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Epididymis (marsupials) ; Ultrastructure ; Sperm maturation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The ductus epididymidis of the tammar is lined by an epithelium composed of principal, mitochondria-rich, apical and basal cells, and intraepithelial leucocytes. The epithelium is structurally differentiated into 6 zones referred to as the initial segment, middle segment (3 subdivisions) and terminal segment (2 subdivisions). The occurrence of the initial, middle and terminal segments corresponds quite closely to the anatomical differentiation of the epididymis into a head, body and tail. The initial segment epithelium in the tammar is lower and has shorter and more slender stereocilia than in other mammals which have been described. Otherwise, the structure of the epithelium has similar characteristics in the tammar to that described in other mammals. Spermatozoa begin to develop the capacity for motility within the initial segment, but only show structural signs of maturation in the middle segment. The sperm head rotates through 90 degrees in the proximal subdivision of the middle segment. The cytoplasmic droplet is detached and spermatozoa develop the capacity for motility in the middle subdivision of the middle segment. The cytoplasmic droplets are phagocytosed by the epididymal epithelium of the middle segment. Sperm storage appears to be the main function of the terminal segment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00228437
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  • 37
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of neuropeptide Y (NPY) in the human hypothalamus (1984)
    Springer
    Cell & tissue research 238 (1984), S. 203-205 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Neuropeptide Y ; Hypothalamus, human ; Immunocytochemistry ; Pituitary stalk
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In order to study the distribution of neuropeptide Y-like immunoreactivity in the human hypothalamus, an immunocytochemical localization of this peptide was performed. Using antibodies developed against synthetic porcine neuropeptide Y (NPY), we have been able to localize immunoreactivity in neuronal cell bodies located exclusively in the infundibular nucleus. Immunostained fibers were found in several regions in the hypothalamus with a high concentration in the periventricular areas. Fibers were also found in the neurovascular zone of the median eminence, the pituitary stalk and the posterior pituitary. These results suggest that immunoreactive material related to porcine NPY is present in the human hypothalamus, with a distribution similar to that observed in the rat.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215163
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  • 38
    Digitale Medien
    Digitale Medien
    Ultrastructural immunocytochemical evidence for peptidergic neurotransmission in the pond snail Lymnaea stagnalis (1984)
    Springer
    Cell & tissue research 238 (1984), S. 197-201 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Peptidergic neurotransmission ; Lymnaea stagnalis ; Immunocytochemistry ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Three neuronal systems of the pond snail Lymnaea stagnalis were immunocytochemically investigated at the ultrastructural level with the unlabeled peroxidase-antiperoxidase technique. Preliminary electrophysiological and cell-filling investigations have shown that a cluster of neurons which reacts positively with an antiserum against the molluscan cardio-active peptide FMRFamide, sends axons to the penis retractor muscle. In this muscle anti-FMRF-amide (aFM) positive axons form neuro-muscular synapses with (smooth) muscle fibers. The morphological observations suggest the aFM immunoreactive system to be involved in peptidergic neurotransmission. In the right parietal ganglion a large neuron (LYAC) is penetrated by aFM positive axons which form synapse-like structures (SLS) with the LYAC. The assumption that the SLS represent the morphological basis for peptidergic transmission is sustained by the observation that iontophoretical application of synthetic FMRFamide depolarizes the LYAC. The axons of a group of pedal anti-vasopressin (aVP) positive cells run in close vicinity to the cerebral ovulation (neuro-)-hormone producing cell system (CDC system) Synapses or SLS between the two systems were not observed. The fact that (bath) application of arg-vasopressin induces bursting in the CDC, may indicate that the vasopressin-like substance of the aVP cells is released non-synaptically.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215162
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  • 39
    Digitale Medien
    Digitale Medien
    Influence of photoperiod on the ultrastructure of the hypophysial pars tuberalis of the Djungarian hamster, Phodopus sungorus (1984)
    Springer
    Cell & tissue research 238 (1984), S. 213-216 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Photoperiods ; Pituitary gland, pars tuberalis ; Ultrastructure ; Phodopus sungorus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Conspicuous cytological differences are found between specific secretory cells of the hypophysial pars tuberalis of Djungarian hamsters exposed to long and short photoperiods. The cells differ with respect to the shapes of perikarya and nuclei and show diverse amounts of secretory granules, lysosome-like bodies and glycogen.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215166
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  • 40
    Digitale Medien
    Digitale Medien
    Ultrastructure and immunolabeling of gonadotrops and thyrotrops in the pituitary of the African catfish, Clarias lazera (1984)
    Springer
    Cell & tissue research 238 (1984), S. 95-103 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pituitary gland, pars anterior (distalis) ; Gonadotrops ; Thyrotrops ; Ultrastructure ; Immunolabeling ; Teleosts
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Pituitaries of the African catfish (Clarias lazera) were studied with immunocytochemical methods, at the light-microscopic and ultrastructural levels, for the characterization and localization of gonadotropic and thyrotropic cells. Two immunostaining procedures with the use of different markers were carried out: (i) with peroxidase-antiperoxidase, (ii) with protein A-gold. In routinely stained sections for light microscopy two types of basophils were identified in the proximal pars distalis: (1) large, round, purple cells, and (2) small, angular, light-blue cells. Both types were immunolabeled with antibodies against Clarias α,β-gonadotropin (GTH) and salmon G100-GTH. Only the large basophils were immunolabeled with anti-carp β-GTH, whereas the small basophils were the only cells immunolabeled with anti-human thyrotropin beta subunit (anti-h TSH-β). It was concluded that the large basophils represent the gonadotrops and the small basophils the thyrotrops. At the ultrastructural level the immunostaining of the GTH-cells was confined to three types of inclusions: (i) secretory vesicles, (ii) globules, and (iii) electron-dense, membrane-bound irregular masses. Especially the protein A- gold method, in combination with the use of a highly diluted homologous antiserum, resulted in a distinct localization of GTH. The presence of two types of nerve fibres, synaptically contacting the gonadotrops, is discussed with regard to the presence of a peptidergic (stimulatory) and an aminergic (inhibitory) control of GTH-secretion.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215149
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  • 41
    Digitale Medien
    Digitale Medien
    Ultrastructural immunocytochemical demonstration of D2-protein in adrenal medulla (1984)
    Springer
    Cell & tissue research 238 (1984), S. 497-502 
    Details
    ISSN: 1432-0878
    Schlagwort(e): D2 glycoprotein ; Adrenal gland ; Immunocytochemistry ; Ultrastructure ; Cell adhesion
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The ultrastructural localization of the glycoprotein D2 in rat adrenal gland was investigated using immunohistochemical methods, and D2 localization in cultures of adult bovine chromaffin cells was studied by immunofluorescence. D2 was found to be situated on nerve fibers passing through the adrenal cortex and in the medulla zone, and also on the surface of all chromaffin cells. In addition, it was strongly expressed on the surface of glial (Schwann) cells. Cortical cells were unreactive to the antiserum. In cultures, all adrenalin and noradrenalin [dopamine-β-hydroxylase (DBH)-positive] cells were surface labelled for D2. A less frequent second cell type was recognized in vitro which was DBH negative but D2 positive. Such cells were presumed to be Schwann cells. These data are discussed in terms of the developmental origin of the cells and with regard to the putative functional rôle of D2 in cell adhesion phenomena.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219864
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  • 42
    Digitale Medien
    Digitale Medien
    The spatial organization of nucleolar DNA in phytohemagglutinin-stimulated lymphocytes of the guinea pig (1984)
    Springer
    Cell & tissue research 235 (1984), S. 65-70 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Lymphocytes ; Phytohemagglutinin stimulation ; Nucleolar organizer region ; Three-dimensional reconstruction ; Ultrastructure ; Guinea pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The ultrastructural changes in the spatial organization of nucleolar DNA in lymphocytes during phytohemagglutinin (PHA) stimulation was studied in guinea pigs by means of oxidized diaminobenzidine (DAB) at low pH as a differentially contrasting stain for nucleic acids and by the use of reconstruction of serial sections. The extended DNA filaments situated inside the fibrillar area originate from a large aggregation of heterochromatin, which is closely associated with the nucleolus, and from the perinucleolar shell of condensed chromatin. It is suggested that these two distinct regions of chromatin might be associated with different functions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213724
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  • 43
    Digitale Medien
    Digitale Medien
    Diurnal variations in myeloid bodies of the newt retinal pigment epithelium (1984)
    Springer
    Cell & tissue research 235 (1984), S. 177-186 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Retinal pigment epithelium ; Myeloid bodies ; Diurnal variation ; Morphometrics ; Ultrastructure ; Lipid metabolism ; Endoplasmic reticulum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Myeloid bodies (MBs) occur in the newt (Notophthalmus viridescens) retinal pigment epithelium (RPE) and are similar to areas of specialized endoplasmic reticulum found in a variety of other cell types. The function of these structures is unknown, although a role in lipid metabolism has been strongly suggested. Random samples from conventionally-fixed and sectioned newt RPE, obtained over a 24-hr cycle (LD 12∶12), were examined by electron microscopy. Myeloid bodies appear as stacks of flattened endoplasmic reticulum-associated saccules which increase in length and number as the RPE accumulates shed outer segment material, prior to increase in the amount of stored lipid. Associations of MBs with the nuclear envelope can be related to this increased length. Myeloid bodies decrease numerically in the cell as phagosomes are removed from the cytoplasm, but a decrease in mean sectional MB area, seen in the light phase, is counteracted in darkness where individual MBs are larger than those found in the light. The total sectional area of MBs within a cell and their mean length varied depending on the lighting condition; differences were also found between eyes after extended periods of continuous light and dark. Ribosomes were found in association with the surfaces of both flattened and circular MBs, but they were consistently more densely associated with the shorter concave surfaces of curved regions. A new hypothesis for MB function is presented, which is concerned with their role in isolating toxic lipids such as retinoids, which are accumulated during phagocytosis of shed outer segment tips, and which are capable of disrupting membrane-bound systems necessary for their eventual metabolism and safe storage.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213738
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  • 44
    Digitale Medien
    Digitale Medien
    Immunohistochemical study of the adenohypophysis of Typhlonectes compressicaudus (Amphibia, Gymnophiona) (1984)
    Springer
    Cell & tissue research 235 (1984), S. 211-214 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Adenohypophysis ; Pars distalis ; Immunocytochemistry ; Amphibia ; Gymnophiona
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The indirect immunofluorescence method was used to identify and locate LTH-, STH-, LH-, TSH-, ACTH- and MSH-immunoreactive cells in the pituitary of Typhlonectes compressicaudus (Gymnophiona). The present study defines the histological and histochemical staining properties of each cell type identified.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213743
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  • 45
    Digitale Medien
    Digitale Medien
    Immunocytochemical demonstration of caldesmon (a calmodulin-binding, F-actin-interacting protein) in smooth muscle fibers and absorptive epithelial cells in the small intestine of the rat (1984)
    Springer
    Cell & tissue research 235 (1984), S. 207-209 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Caldesmon ; Actin ; Immunocytochemistry ; Small intestine ; Smooth muscle ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The distribution of caldesmon (a calmodulin-binding, F-actin-interacting protein) (Sobue et al. 1982) and of actin was studied in the rat's small intestine by means of light-microscopic immunocytochemistry. Positive immunostaining for caldesmon was seen in smooth muscle cells of the intestinal wall, and of blood vessels, and in the apical portion of the absorptive epithelial cells. The immunoreactivity in goblet cells was difficult to recognize. The positive reaction to immunostaining for actin showed almost the same pattern as that for caldesmon. These results suggest that this calmodulin-binding protein may play an important role in the control of actin-myosin interaction in smooth muscle cells and in non-muscle cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213742
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  • 46
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of neurons in the nervous system of the Colorado potato beetle with antisera against FMRFamide and bovine pancreatic polypeptide (1984)
    Springer
    Cell & tissue research 235 (1984), S. 303-308 
    Details
    ISSN: 1432-0878
    Schlagwort(e): FMRFamide ; Bovine pancreatic polypeptide ; Immunocytochemistry ; Peptidergic neurons ; Leptinotarsa decemlineata
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Particular neurons in the nervous system of the Colorado potato beetle, Leptinotarsa decemlineata, are recognized by antisera against bovine pancreatic polypeptide and FMRFamide. Both antisera react with the same neurons. Solid phase absorptions showed that antiserum against bovine pancreatic polypeptide cross-reacts with FMRFamide, whereas antiserum against FMRFamide cross-reacts with bovine pancreatic polypeptide. Some of the immunoreactive neurons have axons branching extensively within the neuropile, which suggests that the peptide is used as transmitter. In the corpus cardiacum, a neurohaemal organ in insects, numerous immunoreactive axon terminals are present. Here, the peptide material is presumably released as a hormone.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217854
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  • 47
    Digitale Medien
    Digitale Medien
    Plasma cells in adult Atlantic hagfish, Myxine glutinosa (1984)
    Springer
    Cell & tissue research 235 (1984), S. 691-693 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Plasma cells ; Ultrastructure ; Immunology ; Myxinoids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Hagfishes, the most primitive vertebrates, are of special interest for the evolution of immune responses. Eptatretus stoutii, the Pacific hagfish, is able to mount cellular and humoral immune responses but all attempts to demonstrate in them the presence of plasma cells have failed. In the present study we demonstrate for the first time plasma cells identifiable by ultrastructural criteria in the pronephros, a primitive lymphohaemopoietic organ, of Myxine glutinosa, the Atlantic hagfish.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226970
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  • 48
    Digitale Medien
    Digitale Medien
    A tubular configuration of the granular endoplasmic reticulum forming a raft-like parallel array in the pinealocytes of two species of Japanese moles (Mogera kobeae and M. wogura) (1984)
    Springer
    Cell & tissue research 236 (1984), S. 15-18 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Granular endoplasmic reticulum ; Ultrastructure ; Pinealocyte ; Mole
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Ten or more straight tubules, each of which consists of a double unit membrane of granular endoplasmic reticulum with a cylindrical profile, are joined side by side in a raft-like configuration in the cytoplasm of the pinealocytes of Japanese moles. They measure about 60 nm and 100 nm in their inner and outer diameters, respectively, and are often partially connected to unspecialized granular endoplasmic reticulum. Cisterns held between the inner and outer unit membranes with cylindrical profiles vary from 15 nm to 30 nm in width. Ensheathed portions of the cytoplasm are contiguous with cytoplasm outside the tubular units. The inner unit membranes of the tubules bear fewer ribosomal particles than the outer ones.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216507
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  • 49
    Digitale Medien
    Digitale Medien
    Satellite cells in the tail muscles of the urodelan larvae during development (1984)
    Springer
    Cell & tissue research 236 (1984), S. 431-438 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Satellite cells ; Satellite fibres ; Tail muscle ; Urodela ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The incidence and ultrastructure of satellite cells in the tail muscles of urodelan larvae were examined during development during which the number of satellite cells is gradually reduced. They are found more frequently in red than in the white fibres in all four stages examined (stage 53, 64, 66+ and juvenile). As development proceeds, intercellular space between satellite cell and muscle fibre is in general gradually extended and is mostly filled with basal lamina. Small muscle cells, satellite fibres, which are situated under the basal lamina of the parent fibre, are morphologically similar to satellite cells but contain a small amount of myofibrils. Three types of satellite fibres are distinguishable on the basis of differences in K2-EDTA-treated ATPase activity, width of Z line, and parent fibre type. Neuromuscular junctions are visible in satellite fibres.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00214247
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  • 50
    Digitale Medien
    Digitale Medien
    Morphometric analysis of loading-induced changes in collagen-fibril populations in young tendons (1984)
    Springer
    Cell & tissue research 236 (1984), S. 465-470 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Tendon ; Collagen fibrils ; Morphometry ; Ultrastructure ; Loading ; Mouse
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary This study was designed to gain more detailed morphological information on skeletal tendons in the course of adaptation to physical loading. The effect on collagen fibrils was investigated in 6-week-old mice by means of electron microscopy. Physical loading was performed on a treadmill 5 days a week for 1, 3, 5, 7 and 10 weeks. Morphometric analysis of collagen fibrils revealed the mean diameter, the diameter distribution, the number and the cross-sectional area. The principal observations included: 1. After one week of physical loading an increase in mean fibril diameter (30%, p≦0.01), in number (15%, p≦ 0.05), and in cross-sectional area (15%, p≦0.05), as well as a change in mean fibril diameter distribution. 2. From the third to the seventh week a fall under the level of the controls in mean diameter (26%, p≦0.01), in number (26%, p≦0.01), and a reduced cross-sectional area (17%, p≦0.01), accompanied by signs of splitting of individual collagen fibrils. 3. In the long-term study an increase in fibril number (29%, p≦0.01), a fall in mean diameter from 189 nm in the controls to 179 nm (p≦0.05) but no statistically significant change in the relative cross-sectional area (32%) per unit in comparison to unloaded tendons. The possible physiological implications of the findings are discussed in the light of several regulatory mechanisms known to appear during the course of physical loading in connective tissues.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00214251
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  • 51
    Digitale Medien
    Digitale Medien
    Localization of corticotropin-releasing factor-containing neurons in the brain of the domestic fowl (1984)
    Springer
    Cell & tissue research 236 (1984), S. 245-248 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Corticotropin-releasing factor ; Immunocytochemistry ; Hypothalamus ; Domestic fowl
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The corticotropin-releasing factor (CRF)-containing neurons were investigated in the brain of the domestic fowl by means of the peroxidase-antiperoxidase technique at the light-microscopic level. The detection of CRF-immunoreactivity was facilitated by silver intensification. CRF-containing perikarya were found in the paraventricular, preoptic and mammillary nuclei of the hypothalamus and in some extrahypothalamic areas (nuclei dorsomedialis and dorsolateralis thalami, nucleus accumbens septi, lobus parolfactorius, periaqueductal gray of the mesencephalon, nucleus oculomotorius ventralis). Immunoreactive nerve fibers and terminals were demonstrated in the external zone of the median eminence and the organum vasculosum of the lamina terminalis. These results indicate that an immunologically demonstrable CRF-neurosecretory system also exists in the avian central nervous system.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216537
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  • 52
    Digitale Medien
    Digitale Medien
    Changes in metabolism and hepatic ultrastructure induced by estradiol and testosterone in immature female Epinephelus akaara (Teleostei, Serranidae) (1984)
    Springer
    Cell & tissue research 236 (1984), S. 651-659 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Steroids ; Vitellogenesis ; Metabolism ; Ultrastructure ; Teleosts
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Estradiol injections increase serum level of calcium, amino acid, glucose, protein, ammonia and creatinine in immature Epinephelus akaara, and also increase levels of total lipid, cholesterol, phospholipid and esterified fatty acids. Hepatic protein, glycogen and lipid concentrations also rise after estradiol treatment, and some hepatic enzymes participating in the metabolism of nitrogen, lipid and carbohydrate, show increased activity. Serum vitellogenin levels are increased. Testosterone treatment increases serum protein, total lipid, cholesterol, amino acid and ammonia levels, and also hepatic glycogen content, but in contrast to estradiol treatment, testosterone does not change serum vitellogenin, glucose, calcium, phospholipid, esterified fatty acid and creatinine levels, nor the hepatic lipid and protein content. A small number of hepatic enzymes shows an increased activity. Vitellogenic fish show biochemical changes similar to that of estradiol-treated fish, but are different from those of immature fish. Estradiol treatment induces ultrastructural changes in the hepatocytes of immature fish that are similar to those found in vitellogenic fish. These include a proliferation of rough endoplasmic reticulum and Golgi apparatus, and an increase in glycogen and lipid, all indicative of enhanced metabolic activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217235
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  • 53
    Digitale Medien
    Digitale Medien
    Synaptic organization of a multifunctional interneuron in the central nervous system of Helix pomatia L. (1984)
    Springer
    Cell & tissue research 236 (1984), S. 677-683 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Interneuron ; Synaptology ; Ultrastructure ; Horseradish peroxidase ; Helix pomatia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The morphology, axonal arborization and ultrastructure of synaptic connections of the V21 giant neuron in the visceral ganglion of the snail Helix pomatia has been investigated following intracellular labelling with horseradish peroxidase. The V21 neuron establishes several afferent and efferent axo-axonic connections, mainly along the first half of the primary axon. Collaterals of 200–300 μm length originate from the primary axon, which shows further arborization, and both afferent and efferent synaptic contacts are formed on these fine axon profiles. Afferent and efferent contacts of the cell occur within very short distances of a few micrometers. On the basis of ultrastructure and vesicle and granule content, several afferent terminals can be distinguished on V21 labelled axon profiles. The majority of these afferent terminals resembles peptidergic-(neurosecretory)-like terminals. This finding supports the possible transmitter role of neuropeptides in the central nervous system of gastropods. Our results are consistent with and provide morphological evidence for recent electrophysiological observations suggesting that, in addition to integrating input, the V21 neuron functions as an interneuron in Helix central nervous system.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217238
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  • 54
    Digitale Medien
    Digitale Medien
    Interactions between immature porcine Leydig and Sertoli cells in vitro (1984)
    Springer
    Cell & tissue research 237 (1984), S. 357-362 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Leydig-Sertoli cell interaction ; FSH stimulation ; Ultrastructure ; Pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Interactions between Leydig and Sertoli cells, as well as a stimulatory effect of FSH on Leydig cell activity, have been reported in many studies. In order to investigate these interactions, the ultrastructure of immature pig Leydig cells under different culture conditions has been studied. When cultured alone in a chemically defined medium, there is a marked regression of the Leydig cell smooth endoplasmic reticulum and a swelling of the mitochondria. Addition of FSH or hCG does not prevent these phenomena. Co-culturing of Leydig cells with Sertoli cells from the same animal maintains the smooth endoplasmic reticulum at the level seen in vivo and in freshly isolated Leydig cells. The addition of FSH to the co-culture stimulates its development and increases Leydig cell activity, as assessed by an increase in hCG binding sites and an increased steroidogenic response to hCG. These results suggest that Sertoli cells exert a trophic effect on Leydig cells, and that the stimulatory effect of FSH on Leydig cell function is mediated via the Sertoli cells. These results reinforce the concept of a local regulatory control of Leydig cell steroidogenesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217157
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  • 55
    Digitale Medien
    Digitale Medien
    Immunocytochemical demonstration of caldesmon and actin in thyroid glands of rats (1984)
    Springer
    Cell & tissue research 237 (1984), S. 375-377 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Thyroid ; Immunocytochemistry ; Caldesmon ; Actin ; Endocytosis ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The distribution of caldesmon (a calmodulin-binding, F-actin interacting protein; Sobue et al. 1982) and actin was studied in the rat thyroid gland by means of light-microscopic immunocytochemistry, and the fine-structural distribution of actin filaments was examined by use of heavy meromyosin (HMM). Caldesmon and actin were demonstrated in the apical cytoplasm of almost all the follicle epithelial cells in normal as well as TSH-treated animals. Immunoreactivities for both caldesmon and actin showed almost the same pattern in localization. The smooth muscle cells of the blood vessels were also positive for caldesmon and actin. By electron microscopy, numerous actin filaments decorated by HMM and running perpendicularly or randomly to the apical surface were recognized in the apical cytoplasm of the follicle epithelial cell. These results suggest that caldesmon and actin, in conjugation with calmodulin, play a role in the regulation of cellular activity such as exocytosis and endocytosis in the apical portion of the follicle epithelial cell.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217161
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  • 56
    Digitale Medien
    Digitale Medien
    Restoration of fast muscle characteristics following cessation of chronic stimulation (1984)
    Springer
    Cell & tissue research 238 (1984), S. 221-230 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Fiber type ; Ultrastructure ; Stereology ; Stimulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary When fast-twitch skeletal muscles of the adult rabbit are subjected to continuous low-frequency activity by electrical stimulation of the corresponding motor nerves, the fibers undergo an ultrastructural transformation, so that after 6 weeks they have acquired an appearance typical of slow-twitch fibers. In the present study, stimulation was discontinued at this stage in order to follow the reverse transformation, in which the fibers recovered their original morphological characteristics under conditions of normal endogenous activity. Stereological techniques were used to assess the time course of this process over a period of 20 weeks in terms of fiber cross-sectional area, extent of T-system, thickness of the Z-band, and volume fraction of mitochondria in the fiber core. Fibers of transformed muscles were smaller than those of control muscles, but the differences were no longer evident after 9 weeks of recovery. After 2 weeks the T-system was still of limited extent, as is characteristic of slow-twitch fibers; it increased toward the amount typical of fast-twitch fibers between 2 and 4 weeks, and had reached its full extent by 12 weeks. The wide Z-bands characteristic of slow-twitch fibers were retained for 4 weeks, but the thickness had begun to decrease by 8 weeks and recovery was complete by 12 weeks. The mitochondrial volume did not increase during recovery, in contrast to the large increases which had been observed to take place between 2 and 6 weeks during the fast-to-slow transformation. Overall, the recovery of fast-twitch ultrastructural characteristics was complete, but followed a more extended time course, and involved less myofibrillar disruption at an intermediate stage, than the original fast-to-slow transformation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217292
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  • 57
    Digitale Medien
    Digitale Medien
    Development and ultrastructural autoradiographic studies of nucleolus-like bodies (nuages) in oocytes of a viviparous teleost (Xiphophorus helleri) (1984)
    Springer
    Cell & tissue research 238 (1984), S. 121-128 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Ultrastructure ; Autoradiography ; Oocytes ; Nucleolus-like bodies ; Teleost
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Cytoplasmic granulo-fibrillar masses, usually termed nucleolus-like bodies (NLB) or nuages, have been described in several different cell types. They are sometimes associated with a mitochondrial arrangement, this association often being marked during certain phases of the oocyte cycle. In Xiphophorus helleri, NLB consist of fibrillar and granular material that gradually becomes more granular during meiotic prophase I, and is associated with mitochondrial arrangements during diplotene and dictyate of meiosis. Autoradiographic studies of uridine incorporation into the nucleolus and subsequently into NLB suggest that the latter represent a reserve of ribonucleoproteins that is later used in ribosomal maturation during vitellogenesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215152
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  • 58
    Digitale Medien
    Digitale Medien
    Ultrastructure of the eyes of the grass shrimp, Palaemonetes pugio (1984)
    Springer
    Cell & tissue research 238 (1984), S. 271-288 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Compound eye ; Ultrastructure ; Grass shrimp, Palaemonetes pugio ; Light adaptation ; Dark adaptation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The cone cells and corneagenous cells possess extensive networks of smooth tubular endoplasmic reticulum that may be involved in optical reflectance and light-adaptational responses, respectively. The extracellular basal lamina of the basement membrane is confluent with glial cell capillary walls and may prove to be a viaduct for the transmission of hemolymph-borne substances to the retina or of retinal degradation products to the hemolymph. In addition to dense pigment granules, the distal pigment cells are shown for the first time to contain migratory reflecting platelets that are usually polymorphic in light-adapted eyes but are rectangular in dark-adapted eyes. In the latter these plates become aligned against the crystalline cones and presumably contribute to the reflection superposition optics of the grass shrimp. Dark-adapted retinular cells possess well-developed perirhabdomal cisternae, oblong or ovoid mitochondria, generally vesicular rough endoplasmic reticulum, and occasional, spherical, calcium-like intrarhabdomal inclusions. Light-adapted retinular cells possess poorly developed perirhabdomal cisternae, lamelliform rough endoplasmic reticulum, and condensed mitochondria frequently associated with lipid droplets and pigment granules. The cytoplasmic boundaries of the reflecting pigment cells expand into the extracellular spaces between individual ommatidial retinular cells during dark adaptation and recede to the interommatidial extracellular spaces during light adaptation. Cytoplasmic microfilament bundles found only at the bases of partially light-adapted rhabdomeric microvilli may be involved in microvillar shortening.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217299
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  • 59
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of urotensin I neurons in the caudal neurosecretory system of the white sucker (Catostomus commersoni) (1984)
    Springer
    Cell & tissue research 235 (1984), S. 19-23 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Urotensin ; Caudal neurosecretory system ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The localization of urotensin I has been investigated in the caudal neurosecretory system of the white sucker (Catostomus commersoni). The peptide is present in all the cells of the system both large and small, in the large axons passing to the urophysis, and in fine beaded fibres not only within the urophysis but also in a fine plexus lateral to the large cells in the spinal cord proper. The possibility that the caudal neurosecretory system is not a functionally uniform system but rather a collection of dissimilar cells of different synaptic inputs with a common entity, urotensin I, is discussed. Moreover, the feasibility of a urotensin I feedback loop is described.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213718
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  • 60
    Digitale Medien
    Digitale Medien
    Comparative immunocytochemical demonstration of ACTH-, LH and FSH-containing cells in the pituitary of neonatal, immature and adult rats (1984)
    Springer
    Cell & tissue research 235 (1984), S. 71-75 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pituitary rat ; LH cells ; FSH cells ; ACTH cells ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary By means of immunocytochemistry, the development of ACTH-, LH- and FSH cells was examined in the anterior pituitary of 5-day-old neonatal, 15-day-old immature and adult rats. ACTH-positive cells are angular and the periphery of these cells is strongly reactive with anti-ACTH serum. In contrast, LH- and FSH-immunopositive cells are ovoid elements, ranging in cell size and intensity of staining. Angular cells, in which only the cell periphery reacted with anti-LHβ serum, were observed in neonatal and immature rats; however, these cells were not stained with either anti-FSHβ serum or anti-ACTH serum. Observation of serial semithin sections revealed that ACTH-immunopositive cells do not react with either anti-LHβ or anti-FSHβ serum. Finally, it was observed that ACTH cells and LH cells are both functionally differentiated already in 5-day-old neonatal rats.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213725
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  • 61
    Digitale Medien
    Digitale Medien
    Ultrastructural immunocytochemical localization of LH and FSH in the pituitary of the untreated male rat (1984)
    Springer
    Cell & tissue research 235 (1984), S. 77-83 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pituitary (rat) ; LH cells ; FSH cells ; Rapid freeze-substitution ; Immunocytochemistry ; Ferritin antibody
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Rapid freeze-substitution fixation was employed in immunocytochemical studies on the localization of LH and FSH in the typical gonadotrophs of the anterior pituitary in the untreated male rat; a modification of a recently described ferritin antibody method (Inoue et al. 1982) was used in these studies. It was shown that rapid freeze-substitution fixation provides good preservation not only of the ultrastructure but also of the antigenicity. Both LH and FSH were clearly demonstrated in the same gonadotrophic cells, but the subcellular localization of these gonadotrophins differed: (i) LH was mainly located in small secretory granules, 250–300 nm in diameter; (ii) FSH was mainly present in large secretory granules, up to 500 nm in diameter. In the pituitary gland of the adult male rat, all gonadotrophs that react to antibodies against gonadotrophins are characterized by small and large secretory granules. Other types of cells of the anterior pituitary containing either small secretory granules or resembling corticotrophs with secretory granules assembled at cell periphery did not react to either anti-LH beta or anti-FSH beta serum. For light microscopy, the peroxidase antibody method was used. All of the gonadotrophin-positive cells contain both LH and FSH. None of the pituitary cells reacted to antibody against only one gonadotrophin. However, some cells are “LH-rich” while other cells are “FSH-rich”.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213726
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  • 62
    Digitale Medien
    Digitale Medien
    Ultrastructural evidence for endogenous testosterone immunoreactivity in the pituitary gland of the rat (1984)
    Springer
    Cell & tissue research 235 (1984), S. 159-169 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Anterior pituitary ; Gonadotropic cells ; Immunocytochemistry ; Testosterone binding ; Cryo-ultramicrotomy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Several attempts have been made to localize steroids by means of immunocytological techniques. However, these methods were found inadequate for detecting steroids bound to their receptors. To localize endogenous testosterone (T) in its target cells at the ultrastructural level, an immunocytological technique was performed on ultrathin sections obtained by cryo-ultramicrotomy. T was detected in the pituitary glands obtained from intact male or female rats and castrated rats, but not in castrated + adrenalectomized rats. Animals were also injected either with testosterone, with other steroids (estradiol, progesterone, corticosterone) or with an androgen antagonist (cyproterone acetate). In addition, some ultrathin sections were preincubated either with phosphate buffers of various pH, corticosterone, cyproterone acetate solution, or with T solution. The content of T in the pituitary before and after fixation was measured by radioimmunoassay; it decreased after fixation. T immunoreactivity was localized in the gonadotropic cells only, both in the male and female rats. At the subcellular level, the immunoreactivity was detected in the cytoplasmic matrix and in the nucleus. Immunoreactive T disappeared 1) in rats after castration+adrenalectomy; by means of radioimmunoassay no T was measured in these pituitary glands; 2) in rats injected with 25 (μg/rat of cyproterone acetate; 3) after preincubation of pituitary sections on a drop of cyproterone acetate (1 × 10-6 M). The immunocytological reaction was not modified when the rats were injected with estradiol, progesterone or corticosterone (1 mg/rat), or after preincubation of the sections with corticosterone (1 × 10-3 M), or a buffer solution at pH 7.6. Lower or higher pH values led to a strong decrease in the immunoreactivity. After injection of T (15 μg/rat) the immunocytological reaction was more abundant in the nucleus and less in the cytoplasm. The immunoreactivity was again observed when the sections were preincubated with cyproterone acetate solution and then with T solution. These data suggest that T can be detected by means of immunocytochemistry. It is probably bound to a specific binding site.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213736
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  • 63
    Digitale Medien
    Digitale Medien
    Evidence that oxytocin-secreting neurones are involved in the ultrastructural reorganisation of the rat supraoptic nucleus apparent at lactation (1984)
    Springer
    Cell & tissue research 235 (1984), S. 217-219 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Immunocytochemistry ; Supraoptic nucleus (SON) ; Oxytocin neurones ; Neuronal appositions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Pre-embedding immunocytochemistry was performed on vibratome sections of the hypothalamus of lactating rats using antiserum directed against oxytocin. Electron microscopy revealed that numerous immunopositive somata and dendrites in the supraoptic nucleus were in direct apposition, without glial interposition; a number of them were also bridged by “double” synapses. The observations support the contention that the ultrastructural reorganisation of the nucleus apparent at lactation affects the magnocellular neurones secreting oxytocin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213745
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  • 64
    Digitale Medien
    Digitale Medien
    Fine structure of submandibular glands of mice with testicular feminization (Tfm/Y) (1984)
    Springer
    Cell & tissue research 235 (1984), S. 295-301 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Tfm/Y mouse ; Submandibular gland ; Sexual ; dimorphism ; Androgens ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The fine structure of the submandibular gland of the mouse with testicular feminization (Tfm/Y) was studied by light and electron microscopy. The architecture of the Tfm/Y gland proved to be rather similar to that of the normal female mouse in both tubular ratio and structure. Granular convoluted tubular cells in Tfm/Y mice characteristically had fewer secretory granules and increased cytoplasmic vacuoles than normal littermates, suggesting an altered synthesis of secretory granules in this cell type of the Tfm/Y mouse. Moreover, there were differences in the ultrastructure of submandibular glands between Tfm/Y and normal female mice. In the gland of the Tfm/Y mouse, basal striations of the striated secretory tubular cells were not so developed and granular intercalated duct cells were less than those of normal females. These findings support the evidence that the secretory tubule of the mouse submandibular gland responds to androgens, resulting in accentuated development in the male, while also suggesting the possibility that the mouse submandibular gland is regulated by other factors which lead to the prominent sexual dimorphism observed in this gland.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217853
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  • 65
    Digitale Medien
    Digitale Medien
    Ultrastructural localization of dipeptidyl peptidase IV in rat salivary glands by immunocytochemistry (1984)
    Springer
    Cell & tissue research 235 (1984), S. 427-432 
    Details
    ISSN: 1432-0878
    Schlagwort(e): DPP IV ; Salivary glands ; Ultrastructural localization ; Immunocytochemistry ; PAP
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The ultrastructural localization of dipeptidyl peptidase IV (DPP IV) (EC 3.4.14.5) in rat submandibular and parotid glands was studied immunocytochemically by the peroxidase-antiperoxidase (PAP) method, using a monospecific antiserum against rat kidney DPP IV. There were no differences in the immunocytochemical localization of DPP IV between submandibular and parotid glands. In these glands, DPP IV was primarily found to be associated with the luminal and intercellular canalicular plasma membranes of acinar cells and with the luminal plasma membranes of intercalated and striated duct cells. Occasionally, immunoreaction of DPP IV was detected in cytoplasmic vesicles (vacuoles), lysosomes, and multivesicular bodies in some acinar cells as well as in ductal epithelial cells. Furthermore, the reaction product was also found within the lumina of peri-acinar and peri-ductal capillaries and in the cytoplasm of some fibroblasts in the interstitial connective tissue. These data suggest that DPP IV in the submandibular and parotid glands may play some role in the secretion or reabsorption processes of secretory proteins and peptides in these glands.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217869
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  • 66
    Digitale Medien
    Digitale Medien
    Invaginated apical vacuoles in the cells of the proximal convoluted tubule in the rat kidney (1984)
    Springer
    Cell & tissue research 235 (1984), S. 463-466 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Endocytosis ; Kidney (rat) ; Proximal tubule ; Apical vacuoles ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Following perfusion fixation of the rat kidney with glutaraldehyde the proximal tubule cells display small apical vacuoles, large apical vacuoles, and apical vacuoles in which a part of the limiting membrane is invaginated into the vacuole. These invaginated apical vacuoles occur more frequently in proximal convoluted tubules than in proximal straight tubules. One tubular cell may contain apical vacuoles of different sizes and stages of invagination, ranging from larger vacuoles with a wide lumen and a small area of invaginated membrane to smaller elements with no apparent lumen and a large area of invaginated membrane. Invaginated apical vacuoles lie either singly in the cytoplasm or close to the membranes of other apical vacuoles, but never in contact with the cell membrane or the membranes of lysosomes, endoplasmic reticulum, Golgi apparatus, mitochondria and peroxisomes. These findings suggest that the invaginated apical vacuoles are not fixation artifacts, but rather develop in living state in cells of the proximal tubule from spherical endocytotic elements.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217875
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  • 67
    Digitale Medien
    Digitale Medien
    Distribution and ultrastructure of S-100-immunoreactive cells in the human thymus (1984)
    Springer
    Cell & tissue research 235 (1984), S. 509-514 
    Details
    ISSN: 1432-0878
    Schlagwort(e): S-100 protein ; Thymus ; Interdigitating cells ; Immunocytochemistry ; Man
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The present study deals with the localization and ultrastructure of S-100-immunoreactive cells in the human thymus. These immunoreactive cells are distributed mainly in the medulla with some scattered elements in the cortex. Electron-microscopic observation revealed that the cells are characterized by an irregularly shaped nucleus, tubulovesicular structures in the cytoplasm and characteristic interdigitations of the plasma membrane. The cells often embrace lymphocytes with their branched processes. On the basis of these morphological features, the immunostained elements were identified as interdigitating cells (IDCs). The immunocytochemistry for S-100 visualizes the precise distribution and extension of the IDCs under the light microscope and indicates that the IDCs form no structural networks such as those established by the thymic epithelial cells. Since the IDCs in human lymph nodes have also been reported to contain S-100-like immunoreactivity, S-100 protein can be regarded as a useful marker for identifying the IDCs in the human thymus and other lymphoid organs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226947
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  • 68
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of a substance in the eyestalk of the prawn, Palaemon serratus, reactive with an anti-FMRF-amide rabbit serum (1984)
    Springer
    Cell & tissue research 235 (1984), S. 601-605 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Immunocytochemistry ; FMRF-amide ; Neurotransmitter ; Palaemon
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary By use of a specific antiserum against the molluscan cardio-excitatory tetrapeptide FMRF-amide in combination with the PAP-method it was possible to obtain positive immunocytochemical reactions in several neurosecretory regions of the eyestalk of the prawn Palaemon serratus. FMRF-amide-like material was found in perikarya and nerve fibers of the medulla terminalis and in neurons in the lamina ganglionaris. The immunoreactivity observed in the glandular tissue located at the basal insertion of the eyestalk muscles must be ascribed to a non-specific reaction. The identification of immunopositive nerve fibers, ending on a nerve bundle in the medulla terminalis, and the fact that immunoreactive material was absent in the neurohemal sinus gland seem to indicate a neurotransmitter/neuromodulator function.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226958
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  • 69
    Digitale Medien
    Digitale Medien
    Differentiation of cells producing polypeptide hormones (ACTH, MSH, LPH, α- and β-endorphin, GH and PRL) in the fetal porcine anterior pituitary (1984)
    Springer
    Cell & tissue research 235 (1984), S. 615-621 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Fetal porcine pituitary ; ACTH, MSH, β-LPH, α- and β-endorphin, GH, PRL ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The aim of the present study on the fetal porcine pituitary was (1) to detect by means of the immunoperoxidase technique the earliest stages of cells producing polypeptide hormones: β-MSH, ACTH, β-LPH, α- and β-endorphin, growth hormone (GH) and prolactin (PRL), (2) to study the development of the synthesis and the storage of these hormones during fetal life, and (3) to detect whether several hormones can be located in one and the same cell. The corticotropic cells were revealed as the earliest functional elements of the fetal anterior pituitary. Our results indicate clearly that ACTH, β-MSH, β-LPH, α- and β-endorphin appear at 34 days in the same regular, round or ovoid cells; no differences in the time of their appearance could be observed. The ACTH-cells, irregular or angular in shape and endowed with cytoplasmic processes such as described in the adult pituitary, were not seen until day 50. The first GH-cells were detected between 40 to 45 days of fetal life. From day 45 to 90, the GH-cells greatly increased in number and in staining intensity of their progressively extending cytoplasmic area, but they displayed the same regular and round shape. The PRL-cells were the last cell type to appear in the fetal pituitary. The first PRL-cells, small in size and round or ovoid in shape with a high nucleus/cytoplasm ratio, were detected at day 70. At day 80, the PRL-cells increased in size and staining intensity. They displayed an irregular elongated or stellated shape and cytoplasmic processes resembling those characteristic of the adult pituitary. These data suggest that in the fetal porcine pituitary: (1) ACTH, β-LPH and related peptides are synthesized and stored in the same cells, and (2) PRL and GH appear in individual cellular elements.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00226960
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  • 70
    Digitale Medien
    Digitale Medien
    The ecdysis of hair mechanoreceptors in crayfish (1984)
    Springer
    Cell & tissue research 236 (1984), S. 339-343 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Moulting ; Mechanosensory hair ; Chordotonal organ ; Ultrastructure ; Crustacea
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The fine structure of hair mechanoreceptors in crayfish during moulting was investigated with special attention to the interface apparatus between cuticular hairs and sensory cells: the chorda. The chordae are lost with old exuviae at every moulting. They are drawn out from a moulting canal at the tip of the new hair. The chordae are regenerated from a material secreted by sheath cells after moulting. Therefore, the chorda is an inward projection of the cuticular exoskeleton, and it has direct contact with the sensory element, the scolopidium. The scolopidium has been found in both hair mechanoreceptors and subcuticular chordotonal organs in crustaceans, and is thought to be a primitive type of mechano-sensory transducing element. The present observation gives additional evidence for the homology of two sensory elements in arthropods, i.e., the cuticular hair sensilla and subcuticular chordotonal organs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00214236
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  • 71
    Digitale Medien
    Digitale Medien
    In vivo shedding of apical plasma membrane in the thyroid follicle cells of the mouse (1984)
    Springer
    Cell & tissue research 236 (1984), S. 87-97 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Iodination ; Membrane shedding ; Peroxidase ; Thyroid follicle cell ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Clusters of luminal dense bodies, limited by a triple-layered membrane, were found in all follicle lumina in thyroid glands of mice. After thyroxine treatment the number of luminal dense bodies increased, especially in the periphery of the lumen, where the intraluminal bodies often displayed a striking resemblance to microvilli. In hyperplastic goiters, obtained by feeding mice with propylthiouracil, luminal dense bodies were replaced by intraluminal vesicles. During goiter involution the vesicles were gradually replaced by luminal dense bodies; the presence of intermediate forms suggests that vesicles and dense bodies are basically the same formations. Luminal dense bodies were observed in colloid droplets indicating their removal by endocytosis. As demonstrated by electron-microscopic cytochemistry, luminal dense bodies contain a membranebound peroxidase, and electron-microscopic autoradiography after administration of 125I indicate that they possess an iodinating capacity. Our observations on mouse thyroid glands suggest that the luminal dense bodies, which appear as vesicles in hyperplastic glands, are formed by shedding of the apical plasma membrane of the follicle cell. The shedding process might be of importance for the turnover of plasma-membrane material.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216517
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  • 72
    Digitale Medien
    Digitale Medien
    Subcellular localization of gonadotropic hormones in pituitary cells of the castrated pig with the use of pre-and post-embedding immunocytochemical methods (1984)
    Springer
    Cell & tissue research 236 (1984), S. 153-160 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Anterior pituitary, porcine ; Gonadotropic hormones (FSH, LH) ; Immunocytochemistry ; Cellular compartments
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Pre- and post-embedding immunocytochemical methods based on the use of specific antibodies against β-subunits of porcine LH and FSH were applied to determine the changes occurring in the anterior pituitary of the pig after gonadectomy. The results showed that (1) the total number of immunoreactive gonadotropes increased from 21–25% in control animals to 24–37% in castrated animals; (2) all gonadotropes contained both LH and FSH; (3) several types of immunoreactive LH/FSH cells were revealed; and (4) the two immunocytochemical methods used with dispersed cells localized the hormones in the same subcellular sites. However, the staining intensity in the different locations varied depending on the method applied. With the post-embedding method, a dense reaction product was found in the secretory granules but the cisternae of RER and the Golgi saccules were always slightly reactive. After the pre-embedding method, the staining intensity in the RER-cisternae and in the Golgi saccules was greatly increased. Thus, the two methodological approaches used in this study have permitted to visualize immunocytochemically the gonadotropic hormones not only at the sites of their storage but also along the intracellular pathway of the secretory material, i.e., at the site of its synthesis and during its passage via the Golgi zone.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216525
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  • 73
    Digitale Medien
    Digitale Medien
    Immunocytochemistry and ultrastructure of the neuropil located ventral to the rat supraoptic nucleus (1984)
    Springer
    Cell & tissue research 236 (1984), S. 171-180 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Immunocytochemistry ; Ultrastructure ; Supraoptic nucleus ; Neuropil ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The neuropil located ventral to the SON was investigated by the use of immunoperoxidase staining for neurophysins, oxytocin and vasopressin, and electron miroscopy. The study was performed in six groups of rats: 1) control; 2) infusion of isotonic saline into the CSF; 3) infusion of hypertonic saline into the CSF; 4) drinking hypertonic saline for 4 days; 5) same as group 4 but injection of colchicine into the CSF on second day of dehydration; 6) salt loading for 3 months. In the control rats the ventral neuropil contained a few immunoreactive processes, the general morphology of which was completely different from that of the neurosecretory axons emerging from the SON at its dorsal aspect. In rats of groups 3 to 6 the ventral processes (VP) became loaded with neurosecretory granules, whereas the perikarya and axons were depleted. Based on their general morphology and reactivity pattern it is suggested that the VP are dendrites. Most of these “dendrites” were embedded in a glial cushion formed by the processes of a particular type of marginal glia. Some of these “dendrites” enveloped an arteriole penetrating the optic tract. All VP were rich in synaptic contacts. The possibility that the VP of neurosecretory cells may be functionally related to the subarachnoid CSF and the arteriolar blood flow is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216528
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  • 74
    Digitale Medien
    Digitale Medien
    Peripolar cell hypertrophy in the renal juxtaglomerular region of newborn sheep (1984)
    Springer
    Cell & tissue research 236 (1984), S. 197-202 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Peripolar cells ; Juxtaglomerular apparatus ; Newborn sheep ; Dexamethasone ; Ultrastructure ; Cytoplasmic granules
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In the renal juxtaglomerular region of newborn sheep, it was found that glomerular peripolar cells and their granules were very much larger than those found in fetal lambs or adult sheep. Similar peripolar cell hypertrophy was triggered in fetal lambs treated in utero with intraperitoneal injections of dexamethasone. Ultrastructurally, granules of peripolar cells from newborn lambs resembled closely the enlarged zymogen granules described in the pancreas of newborn rats. Such peripolar cell hypertrophy may reflect a functional adaptation of the kidney to immediate postnatal life.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216531
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  • 75
    Digitale Medien
    Digitale Medien
    Proliferation and differentiation of intestinal epithelial cells during development of Barbus conchonius (Teleostei, Cyprinidae) (1984)
    Springer
    Cell & tissue research 236 (1984), S. 207-216 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Development ; Enterocytes ; Fish ; Mitosis ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The processes of proliferation, cell division and differentiation of intestinal epithelial cells have been studied during development of the fish, Barbus conchonius. On the 3rd day, nearly all cells of the presumptive gut proliferate. Once the intestinal epithelium begins to differentiate, a decreasing percentage of proliferative cells can be found. On the 7th day, when intestinal folds start to develop, the proliferative cells become restricted to the future basal parts of the folds. Ultrastructural examination of 3H-thymidine-labeled cells and mitotic cells of 6-day-old larvae shows that functional enterocytes are proliferative. The same feature is suggested for older fish. Proliferating undifferentiated “dark” cells, characterized by many free ribosomes and a few organelles, are also present in the intestinal epithelium of larval fish; they are considered to be stem cells, mainly for goblet cells. Proliferating goblet cells and enteroendocrine cells were not observed. The latter cell type is scarce and has a long turnover time. A common feature of all these dividing cells is the presence of isolated spherical to cylindrical lamellar structures which may have lost contact with the cell membrane during prophase; they probably regain this contact by fusion with the cell membrane at the end of mitosis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216533
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  • 76
    Digitale Medien
    Digitale Medien
    Quantitative analysis of ACTH-immunoreactive cells in the anterior pituitary of young spontaneously hypertensive and normotensive rats (1984)
    Springer
    Cell & tissue research 236 (1984), S. 229-235 
    Details
    ISSN: 1432-0878
    Schlagwort(e): ACTH cells ; Immunocytochemistry ; Morphometry ; Spontaneous hypertension
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary ACTH-immunoreactive cells in the anterior pituitary of 4-week-old spontaneously hypertensive rats (SHR) were studied with immunocytochemical and morphometric techniques. The results were compared with data from age-matched normotensive Wistar-Kyoto rats (WKY). No significant differences were found in volume density and average size of ACTH-immunoreactive cells between these two strains. However, SHR showed a significantly larger anterior lobe (2 P 〈 0.01) than WKY, indicating that the total number of ACTH-immunoreactive cells in the anterior pituitary is greater in SHR than in WKY. These data are in agreement with radioimmunological determinations showing a significantly elevated content (2 P 〈 0.01) but only a moderately higher concentration (0.05 〈 2 P 〈 0.10) of ACTH in the anterior pituitary of SHR as compared to WKY. The present results suggest an enhanced availability of ACTH in the anterior pituitary of 4-week-old SHR, a fact which could explain the markedly enhanced stress-induced release of ACTH previously found in these animals. This study further supports the hypothesis that, among other factors, an instability of the hypothalamo-pituitary-adrenal axis may contribute to the development of genetically programmed hypertension.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216535
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  • 77
    Digitale Medien
    Digitale Medien
    Distribution of actin in migrating leukocytes in vivo (1984)
    Springer
    Cell & tissue research 236 (1984), S. 517-525 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Bone marrow ; Actin ; Cell motility ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Blood cells proliferate extravascularly in the bone marrow and enter the circulation by migrating through endothelial cells of venous blood sinuses. This migration, or diapedesis, was suspected to involve actin. To test for the presence and distribution of actin, sections of rat bone marrow were examined by indirect immunocytochemistry. Affinity purified rabbit antichicken gizzard actin antibody, and goat-antirabbit IgG-FITC, or goat antirabbit IgG colloidal gold probes were used. The migrating cell contacts the endothelial cell and forms a podosome (a cortical bleb). Immunocytochemistry shows this region to contain actin. As diapedesis proceeds the podosome deforms, then breaches the endothelial cell. At this time the anterior portion of the leukocyte shows heavy labeling for actin. When the migratory cell traverses approximately half of its length through the endothelial cell, actin appears prominent in the caudal region of the cell. The immunocytochemical data suggest that actin is nonrandomly distributed in leukocytes undergoing diapedesis and may be a component of the force-generating mechanism responsible for this transcellular migratory event.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217218
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  • 78
    Digitale Medien
    Digitale Medien
    Morphometric and fine-structural studies of rat pancreatic acinar cells during early postnatal life (1984)
    Springer
    Cell & tissue research 237 (1984), S. 123-129 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pancreas, exocrine (rat) ; Ultrastructure ; Morphometry ; Development, ontogenetic ; Zymogen granules
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Pancreatic acinar cells of rats obtained at 1,2, 3, 5, 7 and 14 days of age were examined using fine structural and morphometric techniques. From 5 days of age onwards, the acinar cells were analysed twice per day, at 20.00 h and 08.00 h. The present study demonstrates changes in the average volume of the cell, nucleus and cytoplasm, and volume densities of various cytoplasmic organelles during the first two weeks after birth. During early postnatal life, the volume density of rER increases, whereas that of zymogen granules decreases. From 5 days of age onwards, the volume densities of these two organelles differ significantly at 20.00 h and 08.00 h. During the first 2–3 days after birth, inclusion body-like structures appear in the cytoplasm of acinar cells; they contain aggregated zymogen granules and, sometimes, amorphous structures or cytoplasmic organelles. These structures also occur in interstitial cells and cells located in the intercalated region between acinar and ductal epithelial cells. Serum level of α-amylase activity is high at birth, compared with other stages during the first three weeks. Degenerating acinar cells and cell debris can be seen in the acinar and ductal lumina during these stages, a feature suggesting holocrine secretion. Cellular polarity appears to be incomplete during the first two or three days after birth.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00229207
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  • 79
    Digitale Medien
    Digitale Medien
    Granulated folliculo-stellate cells and growth hormone cells immunostained with anti-S 100 protein serum in the pituitary glands of the goat (1984)
    Springer
    Cell & tissue research 237 (1984), S. 7-14 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pituitary gland ; Goat ; Folliculo-stellate cell ; GH cell ; S-100 protein ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Goat pituitary glands were immunohistochemically studied with antisera for bovine S-100 protein, rat LHβ, FSH, TSHβ, prolactin, ovine GH, and porcine ACTH1–39 by use of the superimposition technique on adjacent sections. Folliculo-stellate (F-S) cells were divided into two categories on the basis of ultrastructural properties: One consisted of a mass of agranular cells in which the pseudolumina were equipped with microvilli and cilia. Elongate gap junctions were often observed among these cells. The other was a group of granulated cells with or without pseudolumina. In this group the gap junctions were shown to be disintegrated. The dense granules 150–250 nm in diameter began to accumulate in the cells. However, neither type of these F-S cells was immunostained for S-100 protein. On the other hand, numerous polygonal, elongate, irregular or stellate cells containing S-100 protein were distributed throughout the gland. Most of them were immunohistochemically identical with the GH cells laden with the secretory granules 250–450 nm in diameter, but some of them were identical to TSH and prolactin cells which immunostained faintly for S-100 protein. This appears to be the first demonstration of GH cells intensely immunostained for S-100 protein.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00229194
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  • 80
    Digitale Medien
    Digitale Medien
    FMRF -amide-like immunoreactivity in brain and pituitary of the hagfish Eptatretus burgeri (Cyclostomata) (1984)
    Springer
    Cell & tissue research 237 (1984), S. 363-366 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Hagfish ; Brain ; Pituitary ; FMRF-amide ; Immunocytochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Paraffin sections of brain and pituitary of the hagfish Eptatretus burgeri were immunostained with an antiserum to FMRF-amide. Immunoreactivity was visible in a large number of neurons in the posterior part of the ventromedial hypothalamus and in long neuronal processes extending cranially from the hypothalamus to the olfactory system and caudally to the medulla oblongata. FMRF-amide-like immunoreactivity was also found in cells of the adenohypophysis. These observations suggest that the hagfish possesses a brain FMRF-amide-like transmitter system and pituitary cells containing FMRF-amide-like material. Antisera to ACTH, α-MSH and pancreatic polypeptide gave no immunoreaction in hagfish brain or pituitary.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217158
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  • 81
    Digitale Medien
    Digitale Medien
    Secretory stages of individual CHH-producing cells in the eyestalk of the crayfish Astacus leptodactylus, determined by means of immunocytochemistry (1984)
    Springer
    Cell & tissue research 237 (1984), S. 291-298 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Crustacean hyperglycemic hormone ; Astacus leptodactylus ; Immunocytochemistry ; Quantitative electron microscopy ; Secretory cycle
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Immunocytochemical staining demonstrates striking differences in staining intensity among individual crustacean hyperglycemic hormone (CHH)-producing cells in the eyestalk of the crayfish Astacus leptodactylus. Based on these differences we arbitrarily subdivided the CHH-cells into three categories representing increasing immunoreactivity respectively: + cells, + + cells, and + + + cells. Electron microscopic investigations reveal that these differences in immunostaining are correlated with differences in the numerical density of the neurosecretory granules in the cytoplasm and that these may reflect differences in activity among the CHH-cells. Morphometric analyses at the light- and electron-microscopic levels indicate that the three distinguished categories of immunopositive cells represent different stages in the CHH-synthesizing process of the cells. The results of the present study demonstrate the application of the PAP-technique at the light-microscopic level as a method to obtain information pertaining to the dynamics of secretory activity of the CHH-cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217148
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  • 82
    Digitale Medien
    Digitale Medien
    Occurrence and distribution of neuropeptide-Y-immunoreactive nerves in the respiratory tract and middle ear (1984)
    Springer
    Cell & tissue research 237 (1984), S. 321-327 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Neuropeptide Y ; Immunocytochemistry ; Respiratory tract ; Ear, middle
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Nerve fibres displaying neuropeptide-Y (NPY) immunoreactivity are abundantly distributed in the respiratory tract of cats, guinea-pigs, rats and mice. Fine beaded NPY fibres were seen in whole-mount spreads of the middle-ear mucosa. In the nasal mucosa and in the wall of the Eustachian tube NPY fibres were numerous around arteries and arterioles but sparse in the vicinity of veins; single fibres were found close to the acini of seromucous glands. In the tracheobronchial wall NPY fibres occurred in the proximity of blood vessels, in the subepithelial layer and in the smooth muscle. Surgical and chemical (6-hydroxydopamine treatment) sympathectomy resulted in disappearance of adrenergic and NPY-containing nerve fibres in the nasal mucosa. Sequential staining with antibodies against dopamine-β-hydroxylase (DBH) and NPY revealed that DBH and NPY occur in the same perivascular nerve fibres in the nasal mucosa. The distribution of NPY fibres in the respiratory tract suggests multiple functions of NPY, such as regulation of local blood flow, glandular secretion and smooth muscle activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217151
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  • 83
    Digitale Medien
    Digitale Medien
    Morphological and pharmacological basis for pulmonary ventilation in Amphiuma tridactylum (1984)
    Springer
    Cell & tissue research 238 (1984), S. 1-12 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Lung ; Amphibia ; Ultrastructure ; Smooth muscle ; Extracellular matrix
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The lung of the giant salamander, Amphiuma tridactylum, is divided into respiratory alveoli by muscular septa that increase the surface area of the lung as well as provide a mechanism for its almost complete collapse during exhalation. The epithelium of the internal surface is of two types: respiratory, composed of a single layer of pneumocytes overlying anastomosing capillaries, and non-respiratory, composed of ciliated cells and mucus-secreting goblet cells. Non-respiratory epithelium covers the apical edges of the septa, whereas the respiratory epithelium lines the alveoli. The smooth muscle of the septa and walls of the lung was studied in preparations of uninflated and acetylcholine-contracted lung. The muscle cells are ultrastructurally similar to other types of smooth muscle but are surrounded by extraordinary amounts of extracellular matrix, containing collagen and elastic fibers and numerous fine fibrils of unknown composition. Smooth muscle in isolated lung strips contracted in a dose-dependent manner when treated with acetylcholine or methacholine; contraction was blocked by atropine. Responses of lung strips to adrenergic agents were limited; only high doses of adrenalin caused slight relaxation of previously contracted muscle. These observations support the hypothesis that contraction of pulmonary smooth muscle is responsible for the ventilatory efficiency of the lung.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215138
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  • 84
    Digitale Medien
    Digitale Medien
    Morphological evidence for the presence of two cell types in the ependyma of the subcommissural organ of the snake, Natrix maura (1984)
    Springer
    Cell & tissue research 238 (1984), S. 407-409 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Subcommissural organ ; Secretory granules ; Secretory process ; Ultrastructure ; Natrix maura (Reptilia, Ophidia)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Two different types of ependymal cells were found in the subcommissural organ (SCO) of Natrix maura. Most secretory cells showed morphological features resembling the general structure and ultrastructure of cells in the SCO of other vertebrates. This report describes a second population of cells lining a portion of the dorsal groove of the SCO. These cells were not selectively stained by chromalum-hematoxylin and, under the electron microscope, they were characterized by scarce surface differentiations, sparse apical cytoplasm and short basal processes. Flat, parallel cisternae of the rough endoplasmic reticulum produced vesicles that appeared to be transported to the well-developed Golgi apparatus. Dense secretory granules about 200 nm in diameter were found in the Golgi region. Similar granules were seen in the vicinity of the apical plasma membrane; some of them opened toward the ventricle. All these characteristics clearly differentiate this cell group from the other secretory cells lining the SCO laterally and ventrally.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217314
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  • 85
    Digitale Medien
    Digitale Medien
    On the nature of the opaque and translucent enamel regions of some macropodinae (Macropus giganteus, Wallabia bicolor and Peradorcas concinna) (1984)
    Springer
    Cell & tissue research 238 (1984), S. 329-337 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Teeth (Macropodinae) ; Enamel (opaque, translucent) ; Ultrastructure ; Enamel hardness
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Teeth of three macropod species, M. giganteus, W. bicolor and P. concinna, have been studied using the techniques of light microscopy, scanning- and transmission-electron microscopy and hardness measurement. Light microscope observations showed that the teeth of these species had a translucent enamel region close to the dentine and an outer opaque enamel region at the tooth's surface. These regions were not related to the presence or absence of tubules which are a characteristic feature of marsupial enamel. Hardness tests showed that the opaque enamel was softer than the translucent enamel. Scanning electron microscope observations revealed that there was no correlation between any particular prism packing or orientation and the opaque and translucent enamel regions. Transmission electron microscope observations showed that the translucent enamel region consisted of well defined prisms and well packed, lath-like crystals, whereas the opaque enamel was disrupted by voids (which ranged in size from enlarged micropores to about 2 μm in diameter in extreme cases) between crystals and some randomly oriented, loosely packed crystals. This disruption within the opaque enamel region was more common at prism boundaries but pockets of disrupted enamel were also found within prisms and interprismatic regions. The opacity of the enamel was caused by scattering of light from the voids. The ultrastructure of the opaque enamel region indicated that this region was hypomineralized; hardness tests and polarized light microscope observations were consistent with these results.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217305
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  • 86
    Digitale Medien
    Digitale Medien
    Co-existence of immunoreactivity to anti-dopamine, anti-serotonin and anti-vasotocin in the cerebral giant neuron of the pond snail Lymnaea stagnalis (1984)
    Springer
    Cell & tissue research 238 (1984), S. 411-412 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Immunocytochemistry ; Biogenic amines ; Vasotocin ; Lymnaea stagnalis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Consecutive sections of certain neurons in the central ganglia of the pond snail Lymnaea stagnalis appear to be immunoreactive to anti-dopamine and anti-serotonin. The Cerebral Giant Neurons stain in addition with antivasotocin. The observations indicate the presence of two biogenic amines within the same neuron and in addition their co-existence with a biologically active peptide.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217315
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  • 87
    Digitale Medien
    Digitale Medien
    Opsin-immunoreactive outer segments of photoreceptors in the eye and in the lumen of the optic nerve of the hagfish, Myxine glutinosa (1984)
    Springer
    Cell & tissue research 238 (1984), S. 515-522 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Retinal photoreceptors ; Opsin ; Optic nerve ; Immunocytochemistry ; Cyclostome, Myxine glutinosa
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Opsin-immunoreactive sites in the eye and optic nerve of the hagfish, Myxine glutinosa, were studied by use of light-microscopic pre- and postembedding peroxidase-antiperoxidase or avidin-biotin-peroxidase techniques, and the immuno-electron-microscopic protein A-gold method. At the light-microscopic level, a strong opsin immuno-reaction was obtained on the outer segments of the photoreceptor cells with sheep and rat antibodies against bovine (rhod)opsin. These outer segments were located in the marginal photoreceptor space and in follicles of the retina, as well as in the tubular lumen of the optic nerve. Ultrastructurally, two classes of outer segments can be distinguished; most of them exhibited a strong antiopsin reaction, while certain elements lacked immunoreactivity with the antisera employed. The protein A-gold particles marked opsin-immunoreactive sites on the photoreceptor membranes. The presence of opsin-immunoreactive material in the retina and optic nerve of the hagfish strengthens the view that this primitive eye lacking a cornea, lens and vitreous body is engaged in light perception. The morphological similarity between the eye and pineal tissue is discussed in connection with the absence of a pineal organ in this species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219867
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  • 88
    Digitale Medien
    Digitale Medien
    Organ culture of the goldfish pineal body (1984)
    Springer
    Cell & tissue research 238 (1984), S. 565-575 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Pineal organ teleost ; Tissue culture ; Ultrastructure ; Indoles ; High performance liquid chromatography (HPLC)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The ultrastructure and biochemistry of the goldfish pineal organ were examined in expiants cultured for 1, 3, and 6 days. All four cell types (photoreceptor, supportive, ganglion, phagocytic) were identified; they exhibited many of the characteristics of these cells in vivo. Exceptions included a gradual disorganization of the outer segments and reduction of synaptic ribbons in photoreceptors with time in culture. In addition, there was a marked proliferation of endoplasmic reticulum in both photoreceptor and supportive cells. The indoles 5-hydroxytryptophan, serotonin, 5-hydroxyindoleacetic acid, 5-methoxytryptophol, and melatonin were separated in expiants by high performance liquid chromatography using electrochemical detection. Serotonin levels could be depleted by p-chlorophenylalanine and elevated by nialamide or by adding 5-hydroxytryptophan to the culture medium. These findings suggest that organ culture may be a useful model for study of regulatory processes related to the photoneuroendocrine functions of the teleost pineal organ.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219873
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  • 89
    Digitale Medien
    Digitale Medien
    Localization of a peptide identified by antibodies to gastrin/CCK in the gut of Cancer magister (1984)
    Springer
    Cell & tissue research 238 (1984), S. 113-119 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Crustacea ; Gastrointestinal hormones ; Neuropeptides ; Immunocytochemistry ; Cuticle ; Cancer magister
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A gastric peptide from the Dungeness crab (Cancer magister), extracted and characterized previously (Larson and Vigna 1983b), was localized in the foregut (stomach) of this species by immunocytochemistry using antisera specific for the bioactive carboxy-terminal amino acid sequence common to gastrins and cholecystokinins (CCKs). Immunoreactivity was found in all gastric epithelial cells and in the procuticle. Electron microscopy revealed an absence of peptidergic secretory granules in the gastric epithelial cells. The pattern of immunostaining suggests that the gastric epithelial cells secrete this peptide apically where it is incorporated into the cuticle lining the lumen. Specific immunostaining could not be demonstrated in various neural ganglia or in the hypodermis. The distribution of this peptide is different from that of gastrin/CCK in vertebrates and other invertebrates. This suggests that the crab gastric peptide is sufficiently similar to gastrin/CCK to react with C-terminal specific antisera, but may be anatomically, functionally, and possibly phylogenetically otherwise unrelated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215151
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  • 90
    Digitale Medien
    Digitale Medien
    Somatostatin in the brain and the pituitary of some teleosts (1984)
    Springer
    Cell & tissue research 238 (1984), S. 289-296 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Somatostatin (SRIF) ; Brain ; Pituitary gland ; Immunocytochemistry ; Teleosts
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Immunocytochemical investigations show that somatostatin (SRIF)-like immunoreactive material is present in the brain and the pituitary of nine different species of teleosts. In the brain, immunoreactive perikarya and fibers are observed in the preoptic periventricular nucleus, the entopeduncular nucleus, the anterior periventricular nucleus, and the nucleus lateralis tuberis. In the pituitary, SRIF-like-immunoreactive fibers occur in the proximal pars distalis (PPD), which contains the growth hormone (GH)-secreting cells. Nerve fibers are scattered among GH cells (cyprinids), or end on the basal lamina at the neuroglandular interface of the PPD (eel, salmonids). In the eel, the proximal neurohypophysis does not penetrate deeply into the PPD that is very poorly vascularized. In some species, e.g. Myoxocephalus, SRIF-like immunoreactive fibers are also observed in the caudal neurohypophysis, and even among MSH cells of the pars intermedia. In long-term starved carps and eels, the amount of SRIF-like material in the pituitary is clearly reduced. A possible role of SRIF in the concomitant stimulation of GH cells is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217300
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  • 91
    Digitale Medien
    Digitale Medien
    Electron-microscopic study of innervation of smooth muscle cells surrounding collecting tubules of the fish kidney (1984)
    Springer
    Cell & tissue research 238 (1984), S. 307-312 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Innervation ; Smooth muscle ; Fish ; Kidney ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The fine structure of the collecting tubules of the trout and killifish kidney was studied. These tubules are surrounded by layers of smooth muscle cells which are commonly innervated. The nerve terminals contain synaptic vesicles and, occasionally, a few dense-cored granules as well. Capillaries occur in the connective tissue space between these smooth muscle cells and the collecting tubule. Epithelial cells of the collecting tubules contain abundant mitochondria and a well developed membrane system displaying parallel arrays, and were considered to be actively involved in the transport of materials. In the trout, the collecting tubules contain peculiar cells in addition to regular tubule cells. The fine structure of these peculiar cells is highly reminiscent of that of gill chloride cells. The significance of these findings may be summarized as follows: If the smooth muscles around the collecting tubule contract under neural influence, intratubular pressure may be increased and, thus affect glomerular filtration rate. The contraction of these muscles may also cause the collapse of peritubular capillaries, affecting the transport activity of tubule cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217302
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  • 92
    Digitale Medien
    Digitale Medien
    Immimocytological localization of a somatostatin-like substance in the brain of the giant slug, Limax maximus L. (1984)
    Springer
    Cell & tissue research 238 (1984), S. 349-353 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Immunocytochemistry ; Slug brain ; Somatostatin-like material ; Maturation hormone
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Immunocytological tests reveal the presence of a somatostatin-like substance in perikarya and axons in the brain of the giant slug Limax maximus L. Controls carried out on adjacent sections with absorbed antiserum or different antibodies raised against several biologically active peptides of vertebrates (ACTH-17-39, α- and β endorphin, α- and β MSH, methionin-enkephalin, TRH) demonstrate the specificity of the “staining”. However, some cells are both somatostatin- and FMRF-amide-positive. In the cerebral ganglia, the right Z-area cells, responsible for the synthesis of the maturation hormone (MH) are strongly somatostatin-positive. These results suggest a similarity between the MH and the somatostatin-like material contained in the Z-area cells. The simultaneous presence of two peptides in one and the same cell, the nature (elementary granules or soluble product) of the material, and its site of release are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00217307
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  • 93
    Digitale Medien
    Digitale Medien
    Immunocytochemical demonstration of peptidergic neurons in the central and peripheral nervous systems of the flatworm Microstomum lineare with antiserum to FMRF-amide (1984)
    Springer
    Cell & tissue research 238 (1984), S. 431-436 
    Details
    ISSN: 1432-0878
    Schlagwort(e): FMRF-amide ; Immunocytochemistry ; Nervous system ; Microstomum lineare (Turbellaria)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The central nervous system (CNS) and the peripheral nervous system (PNS) of the flatworm Microstomum lineare were studied by means of the peroxidase-antiperoxidase (PAP) immunocytochemical method, with the use of antisera to the molluscan cardioactive peptide FMRF-amide. FMRF-amide immunoreactive perikarya and nerve fibres are observed in the CNS and the PNS. In the CNS, immunoreactive perikarya and nerve fibres occur in the brain, in the epithelial lining and the mesenchymal surroundings of the ciliated pits, and positive fibres in the longitudinal nerve cords. In the PNS, immunoreactive fibre bundles with variocosities occur in the pharyngeal nerve ring, in symmetrical groups of perikarya on each side of the pharynx, and in the mouth area. Positive perikarya and meandering nerve fibres appear in the intestinal wall. A few immunoreactive cells and short nerve processes are observed at the male copulatory organ and on both sides of the vagina. Some immunoreactive peptidergic cells do not correspond to cells previously identified by histological techniques for neurosecretory cells. The distribution of immunoreactivity suggests that the FMRF-amide-like substance in CNS and PNS in this worm has roles similar to those of the brain-gut peptides in vertebrates. The status of FMRF-amide-like peptides as representatives of an evolutionarily old family of peptides is confirmed by the positive immunoreaction to anti-FMRF-amide in this primitive microturbellarian.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219857
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  • 94
    Digitale Medien
    Digitale Medien
    Ultrastructural identification of catecholaminergic fibers in the goldfish pituitary (1984)
    Springer
    Cell & tissue research 238 (1984), S. 621-626 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Catecholamines ; Pituitary innervation ; Radioautography ; Ultrastructure ; Goldfish
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The monoaminergic innervation of the goldfish pituitary gland was studied by means of light- and electronmicroscopic radioautography after in vitro administration of 3H-dopamine. The tracer was specifically incorporated and retained by part of the type-B fibers innervating the different lobes of the pituitary. In the rostral pars distalis labeled fibers were most frequently observed in contact with the basement membrane separating the neurohypophysis and the adenohypophysis. In the proximal pars distalis and the pars intermedia, labeled profiles were detected in the neural tissue and in direct contact with the different types of secretory cells. According to the previous data concerning the uptake and retention of tritiated catecholamines in the central nervous system, it is assumed that the labeled fibers are mainly catecholaminergic (principally dopaminergic). This study provides morphological evidence for a neuroendocrine function of catecholamines in the goldfish.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219880
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  • 95
    Digitale Medien
    Digitale Medien
    Peroxidase-positive endothelial cells in rat liver (1984)
    Springer
    Cell & tissue research 238 (1984), S. 635-642 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Liver ; Endothelium ; Kupffer cells ; Peroxidase ; Cytochemistry ; Ultrastructure ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Rat liver fixed by perfusion with low glutaraldehyde concentrations was incubated in diaminobenzidine-containing medium to stain for peroxidase. Endogenous peroxidatic activity was found not only in Kupffer cells but also in the endothelial cells lining the sinusoids and central veins. The reaction product was localized in the nuclear envelope and endoplasmic reticulum. The peroxidatic activity in endothelial cells showed a concentration-dependent sensitivity to glutaraldehyde: in liver samples fixed with 0.25% glutaraldehyde, approx. 23% of the sinusoidal endothelial cells and 65% of central vein endothelium were peroxidase-positive; with 0.5% glutaraldehyde, only approx. 8% of the sinusoidal endothelial cells contained detectable amounts of the reaction product; with 1.5% glutaraldehyde all endothelial cells were consistently peroxidase-negative. No peroxidatic activity could be found in liver endothelial cells following isolation by centrifugal elutriation. Endothelial cell peroxidase may possibly be involved in defense responses of liver and/or, as a part of prostaglandin synthase system, in prostanoid production.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00219882
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  • 96
    Digitale Medien
    Digitale Medien
    OnHapaline appendiculata andPhymatarum borneense, two rareAraceae from Borneo (1984)
    Springer
    Plant systematics and evolution 144 (1984), S. 59-66 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Araceae ; Hapaline ; Hapale ; Phymatarum ; Bucephalandra ; Flora of Borneo ; East Malaysia ; Sarawak
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract On the basis of newly collected and cultivated material full descriptions and illustrations are presented forHapaline appendiculata Ridl. andPhymatarum borneense M. Hotta.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00990801
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  • 97
    Digitale Medien
    Digitale Medien
    Veronica fridericae, a new species from eastern Turkey (1984)
    Springer
    Plant systematics and evolution 144 (1984), S. 67-71 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Scrophulariaceae ; Veronica fridericae spec. nova ; V. acrotheca ; V. farinosa ; V. armena ; V. multifida ; V. jacquinii ; V. caucasica ; V. filifolia ; V. oltensis ; Flora of Turkey
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Veronica fridericae spec. nova, described from the type locality in the S.E. Turkish province Van, is related toV. acrotheca andV. farinosa, both endemic to W. Iran. All 8 perennial species with pinnatifid leaves in S.W. Asia are presented in a key.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00990802
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  • 98
    Digitale Medien
    Digitale Medien
    Eine neueCampanula-Art aus dem ostafghanisch-westpakistanischen Grenzgebiet (1984)
    Springer
    Plant systematics and evolution 144 (1984), S. 149-150 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Campanulaceae ; Campanula schimaniana spec. nova ; Flora of Afghanistan and of Pakistan
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Campanula schimaniana is a new species of sect.Campanula subsect.Rupestres, distributed in the westernmost mountains affected by monsoon.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00986672
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  • 99
    Digitale Medien
    Digitale Medien
    Chromosome counts inCalea (Asteraceae-Heliantheae) (1984)
    Springer
    Plant systematics and evolution 144 (1984), S. 151-153 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Compositae ; Asteraceae-Heliantheae ; Calea ; Chromosome numbers ; polyploidy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Chromosome numbers are reported for six taxa ofCalea, among them tetraploidC. septuplinervia, the only known polyploid inCalea s. str. The base chromosome number ofCalea is interpreted as x = 19.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00986673
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  • 100
    Digitale Medien
    Digitale Medien
    Immunosystematical comparison ofPhaseolus coccineus seed proteins with those of other legumes (1984)
    Springer
    Plant systematics and evolution 144 (1984), S. 155-163 
    Details
    ISSN: 1615-6110
    Schlagwort(e): Angiosperms ; Leguminosae ; Fabaceae ; Caesalpinioideae ; Mimosoideae ; Faboideae ; Phaseolus coccineus ; Serological systematics ; seed proteins ; phytohaemagglutinin ; vicilin ; phaseolin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract An immunosystematical research was conducted on the seed proteins of 32 species ofFabaceae. By comparing all immunelectrophoretic patterns with the self reaction of a reference system (Phaseolus coccineus) all proteins detected have been identified and their distribution within the family has been analyzed. Half of the proteins identified inP. coccineus gave positive cross reactions with proteins present in all other species. Among this group are “protein I” and “phaseolin”. This result supports the homologization between phaseolin and vicilin. Among the other proteins, three are irregularly distributed throughout the family, and only three are restricted to a few taxa. This last group includes phytohaemagglutinin, which does not present any cross reaction outside the tribePhaseoleae.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00984130
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