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  • 1
    Electronic Resource
    Electronic Resource
    Expression of connexin 43 in human testis (1999)
    Springer
    Histochemistry and cell biology 112 (1999), S. 215-220 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  In order to further characterize the Sertoli cell state of differentiation, we investigated the expression of connexin 43 (cx43) protein in the testis of adult men both with normal spermatogenesis and associated with spermatogenic impairment, since cx43 is first expressed during puberty. Cx43 protein was found as a single 43-kDa band on western blots of extracts of normal human testicular material. Cx43 immunoreactivity was generally present between Leydig cells. Within the normal seminiferous epithelium cx43 immunoreactivity was localized between adjacent Sertoli cells, except at stages II and III of the seminiferous epithelial cycle when primary spermatocytes cross from the basal to the adluminal compartment suggesting a stage-dependent Sertoli cell function. While testes with hypospermatogenesis and spermatogenic arrest at the level of round spermatids or spermatocytes revealed a staining pattern similar to that of normal adult testis, the seminiferous tubules showing spermatogenic arrest at the level of spermatogonia and Sertoli-cell-only syndrome were completely immunonegative. We therefore assume that severe spermatogenic impairment is associated with a population of Sertoli cells exhibiting a stage of differentiation deficiency.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004180050409
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  • 2
    Electronic Resource
    Electronic Resource
    Seasonal distribution of sympagic amphipods near Chesterfield Inlet, N.W.T., Canada (1997)
    Springer
    Polar biology 18 (1997), S. 16-22 
    Details
    ISSN: 1432-2056
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The seasonal distribution of sympagic amphipods was investigated in the Chesterfield Inlet area of northwestern Hudson Bay (63°30′N). Amphipod abundance was measured by photographic samples and species composition was determined by sweep net samples. Twelve species of amphipods were collected, the most common being Ischyrocerus anguipes, Pontogeneia inermis, Apherusa megalops and Weyprechtia pinguis. The major environmental variable affecting amphipod distribution was water depth. Amphipod abundance was highest near 20 m and near zero past 50 m. The maximum recorded abundance was 1367 m−2. A minor factor affecting the distribution of amphipods was snow depth, through its modifying effect on light and thereby the growth of ice algae. Amphipods began to inhabit the sea ice shortly after its formation. From the beginning of March, the number of amphipods on the ice increased steadily to about the 3rd week of April, after which numbers declined. This pattern coincided with the seasonal ice algae abundance. Amphipods reduced ice algal biomass over 20-m depth by 63%. No evidence of diurnal changes in abundance was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003000050154
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  • 3
    Electronic Resource
    Electronic Resource
    Localization of inhibin/activin subunits in the testis of adult nonhuman primates and men (1993)
    Springer
    Cell & tissue research 273 (1993), S. 261-268 
    Details
    ISSN: 1432-0878
    Keywords: Inhibin ; Activin ; Testis ; Human ; Macaca fascicularis, Macaca mulatta, Macaca arctoides (Primates)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The localization and distribution of inhibin/activin subunits was evaluated in the testes of three nonhuman primate species (Macaca fascicularis, M. mulatta, M. arctoides), of young (31 to 43 years) and old (60 to 85 years) men, and of men with disturbed or arrested spermatogenesis using immunohistochemical techniques (peroxidase-anti-peroxidase and alkaline-phosphatase/ anti-alkaline-phosphatase technique). Specific polyclonal (anti-porcine inhibin α-1-32 and anti-bovine activin A) and monoclonal (anti-human inhibin α-1-32 and anti-human activin βA-82-114) antisera were employed. Among all nonhuman primate species and in men, inhibin/activin subunits were present in the cytoplasm of Sertoli cells and Leydig cells but not in germ cells. No relationship could be established between the staining pattern for inhibin/activin subunits and the completeness or the stage of the spermatogenic process. The staining for the βA-subunit in Sertoli cells appeared more intense in the testes of old men compared with that of young men. The majority of Leydig cells contained either the α-subunit and βA-subunit or the βA-subunit alone. The signal for the βA-subunit was remarkably intense in normal and hyperplastic human Leydig cells. These observations demonstrate the presence of inhibin/activin subunits in Sertoli cells and Leydig cells of adult primates and raise the possibility that these subunits or their respective dimers (inhibin A/activin A) might subserve a paracrine/ autocrine role in the adult primate testis. Also, the possibility of specific differences in the α-1-32 subunit and the βA-82-114 subunit region among certain primate species arises from the observation that the monoclonal antisera failed to detect the respective antigens in M. fascicularis and M. mulatta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00312827
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  • 4
    Electronic Resource
    Electronic Resource
    Localization of follicle-stimulating hormone (FSH) immunoreactivity and hormone receptor mRNA in testicular tissue of infertile men (1994)
    Springer
    Cell & tissue research 278 (1994), S. 595-600 
    Details
    ISSN: 1432-0878
    Keywords: FSH ; Immunohistochemistry ; Receptor mRNA ; In situ hybridization ; Sertoli cell ; Testis ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Testicular biopsies from 82 oligo-or azoospermic male patients were subjected to immunostaining using anti-human FSH antibodies. Histological evaluation showed normal spermatogenesis (nspg) in 7 (FSH: 2.7±0.7), mixed atrophy (ma) in 63 (FSH:5.3±0.5), and bilateral or unilateral Sertoli Cell Only syndrome (SCO) in 12 (FSH:21.7±3.5) patients. For the relationship between FSH values and testicular histology, see Bergmann et al. (1994). FSH immunoreactivity was found exclusively in Sertoli cells and in some interstitial cells. Seminiferous epithelium showing normal or impaired spermatogenesis displayed only weak immunoreactivity compared to intense immunoreaction, i.e. large and numerous vesicles in Sertoli cells of SCO tubules in biopsies showing mixed atrophy or SCO. In addition, h-FSH receptor mRNA was demonstrated by in situ hydridization using biotinylated cDNA antisense oligonucleotides. Hybridization signals were found within the seminiferous epithelium exclusively in Sertoli cell cytoplasm associated with normal spermatogenesis and in epithelia showing different signs of impairment, including SCO. It is concluded that: (1) Sertoli cells are the only cells within the seminiferous epithelium expressing FSH receptors; (2) the accumulation of FSH immunoreactivity in Sertoli cells of SCO tubules appears to be a sign of impaired Sertoli cell function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331379
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  • 5
    Electronic Resource
    Electronic Resource
    Localization of follicle-stimulating hormone (FSH) immunoreactivity and hormone receptor mRNA in testicular tissue of infertile men (1994)
    Springer
    Cell & tissue research 278 (1994), S. 595-600 
    Details
    ISSN: 1432-0878
    Keywords: Key words: FSH ; Immunohistochemistry ; Receptor mRNA ; In situ hybridization ; Sertoli cell ; Testis ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Testicular biopsies from 82 oligo- or azoospermic male patients were subjected to immunostaining using anti-human FSH antibodies. Histological evaluation showed normal spermatogenesis (nspg) in 7 (FSH: 2.7±0.7), mixed atrophy (ma) in 63 (FSH: 5.3±0.5), and bilateral or unilateral Sertoli Cell Only syndrome (SCO) in 12 (FSH:21.7±3.5) patients. For the relationship between FSH values and testicular histology, see Bergmann et al. (1994). FSH immunoreactivity was found exclusively in Sertoli cells and in some interstitial cells. Seminiferous epithelium showing normal or impaired spermatogenesis displayed only weak immunoreactivity compared to intense immunoreaction, i.e. large and numerous vesicles in Sertoli cells of SCO tubules in biopsies showing mixed atrophy or SCO. In addition, h-FSH receptor mRNA was demonstrated by in situ hybridization using biotinylated cDNA antisense oligonucleotides. Hybridization signals were found within the seminiferous epithelium exclusively in Sertoli cell cytoplasm associated with normal spermatogenesis and in epithelia showing different signs of impairment, including SCO. It is concluded that: (1) Sertoli cells are the only cells within the seminiferous epithelium expressing FSH receptors; (2) the accumulation of FSH immunoreactivity in Sertoli cells of SCO tubules appears to be a sign of impaired Sertoli cell function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050250
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  • 6
    Electronic Resource
    Electronic Resource
    The blood-testis barrier in vertebrates having different testicular organization (1984)
    Springer
    Cell & tissue research 238 (1984), S. 145-150 
    Details
    ISSN: 1432-0878
    Keywords: Blood-testis barrier ; Tight junctions ; Lanthanum tracer ; Testis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The occurrence of tight junctions between Sertoli cells, providing the structural basis of the blood-testis barrier, has been studied using hypertonic fixative and lanthanum tracer in the testes of seven species of vertebrates having different testicular organization. In all cases inter-Sertoli tight junctions, establishing an effective barrier, appear either when meiosis is complete (teleosts and amphibians, both with cystic testes) or immediately after the onset of meiosis (reptiles and birds, both having testes consisting of seminiferous tubules). In the cystic testes, tight junctions are regularly associated with desmosomes, whereas in testes with seminiferous tubules, cisternae of the endoplasmic reticulum are present beneath the junctions (subsurface cisternae). The avian testes examined have, in addition, septate-like junctions between the Sertoli cells but before the tight junctions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215155
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  • 7
    Electronic Resource
    Electronic Resource
    Observations on the blood-testis barrier in a frog and a salamander (1983)
    Springer
    Cell & tissue research 232 (1983), S. 189-200 
    Details
    ISSN: 1432-0878
    Keywords: Blood-testis barrier ; Lanthanum tracer ; Frog ; Salamander
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A blood-testis barrier has been demonstrated in a frog, Rana esculenta, and in a salamander, Salamandra salamandra, using lanthanum as an electron-dense marker during fixation. The tracer penetrates the interstices between somatic follicle or Sertoli cells and germ cells in regions of the testis containing spermatogonia and spermatocytes, up to the level of punctate tight junctions. The latter can be localized between the somatic cells that line seminiferous units containing spermatids and mature spermatozoa. The barrier thus appears to be established after meiosis in both species investigated, although spermatids of different developmental stages can be found in open compartments of the testis in S. salamandra.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222383
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  • 8
    Electronic Resource
    Electronic Resource
    Influence of photoperiod on the ultrastructure of the hypophysial pars tuberalis of the Djungarian hamster, Phodopus sungorus (1984)
    Springer
    Cell & tissue research 238 (1984), S. 213-216 
    Details
    ISSN: 1432-0878
    Keywords: Photoperiods ; Pituitary gland, pars tuberalis ; Ultrastructure ; Phodopus sungorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Conspicuous cytological differences are found between specific secretory cells of the hypophysial pars tuberalis of Djungarian hamsters exposed to long and short photoperiods. The cells differ with respect to the shapes of perikarya and nuclei and show diverse amounts of secretory granules, lysosome-like bodies and glycogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215166
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  • 9
    Electronic Resource
    Electronic Resource
    Differential permeability of pineal capillaries to lanthanum ion in the rat (Rattus norvegicus), gerbil (Meriones unguiculatus) and golden hamster (Mesocricetus auratus) (1985)
    Springer
    Cell & tissue research 241 (1985), S. 149-154 
    Details
    ISSN: 1432-0878
    Keywords: Capillary permeability ; Lanthanum tracer ; Pineal gland ; Rodents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Capillaries in the pineal gland of the rat (Rattus norvegicus), gerbil (Meriones unguiculatus) and golden hamster (Mesocricetus auratus) were investigated by means of electron-microscopical tracer studies using lanthanum. The tracer was administered together with the fixative solution by perfusion via the left cardiac ventricle. In the rat, endothelial junctions of fenestrated capillaries are permeable to lanthanum. In the gerbil the tracer does not leave the capillaries, which are of the non-fenestrated type throughout the organ. In the golden hamster the two portions of the pineal system have different types of capillaries. While in the superficial pineal fenestrated capillaries permeable to lanthanum predominate, the deep pineal possesses capillaries that are, without exception, devoid of fenestrations and impermeable to the tracer. It is suggested that differences in the structure of the capillaries are related to differences in the extent of a specialized contact of the pineal gland to the third ventricle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214636
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  • 10
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    Eine diskursanalytische Betrachtung des rok-samizdat in der Sowjetunion und ihren Nachfolgestaaten in der Periode zwischen 1967 und 1994 (2021)
    Peter Lang International Academic Publishing Group
    Details
    Publication Date: 2024-03-24
    Description: In the present work, the phenomenon of roc - samizdat in the Soviet Union and its successor states in the period between 1967 and 1994 is considered in terms of discourse analysis. In the first part of the thesis the used discourse analytical instruments are presented, in the second part the same is applied to selected texts. The rok-samizdat represents a form of samizdat, which, as will be shown in the present work, is thematically focused on the field of (rock) music.
    Description: In der vorliegenden Arbeit wird das Phänomen des rok - samizdat in der Sowjetunion und deren Nachfolgestaaten in der Periode zwischen 1967 und 1994 diskursanalytisch betrachtet. Im ersten Teil der Arbeit wird das verwendete diskursanalytische Instrumentarium dargestellt, im zweiten Teil wird selbiges auf ausgewählte Texte angewendet. Der rok-samizdat stellt eine Form des samizdat dar, der, wie im Laufe der vorliegenden Arbeit gezeigt werden soll, thematisch schwerpunktmäßig auf den Bereich der (Rock-) Musik beschränkt ist.
    Keywords: 1967 ; 1994 ; Bergmann ; Betrachtung ; Die Wort-Satz-Relation ; diskursanalytische ; Eine ; ihren ; konstruktiver Funktionalismus ; Kulturstratifíkation ; Nachfolgestaaten ; Nicht-interdiskursive Sprachelemente ; Periode ; samizdat ; Sowjetunion ; Zweite Wirklichkeit ; zwischen ; thema EDItEUR::C Language and Linguistics::CF Linguistics
    Language: German
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