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  • Articles  (544)
  • gene expression  (307)
  • temperature  (237)
  • Springer  (544)
  • American Association for the Advancement of Science
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  • 1995-1999  (544)
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  • 1
    Electronic Resource
    Electronic Resource
    Langfristige Zunahme früher Bruten beim Teichrohrsänger (Acrocephalus scirpaceus) in einem südwestdeutschen Untersuchungsgebiet (1999)
    Springer
    Journal of ornithology 140 (1999), S. 81-86 
    Details
    ISSN: 1439-0361
    Keywords: Reed Warbler ; timing of breeding ; spring arrival ; global climate change ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung In einem Untersuchungsgebiet am westlichen Bodensee (Halbinsel Mettnau) zeichnet sich zwischen 1976 und 1997 eine deutliche Zunahme früh ausgeflogener Teichrohrsänger ab, was auf ein früheres Brüten schließen läßt. Weiterhin hat sich die Erstankunft des Teichrohrsängers am Bodensee in den letzten 16 Jahren (1982–1997) im Vergleich zum Zeitraum 1961 bis 1980 um genau eine Woche verfrüht (im Mittel vom 26.4. auf den 19.4.). Während sich zwischen der jährlichen Erstankunft des Teichrohrsängers am Bodensee und der Zahl früh ausgeflogener Jungvögel kein statistisch gesicherter Zusammenhang finden läßt, ist die Temperatur in der ersten Maihälfte ein guter (statistisch signifikanter) Indikator für die Zahl früh ausgeflogener Teichrohrsänger. Die vorliegende Untersuchung zeigt, daß sich offenbar auch Langstreckenzieher an die sich abzeichnende Klimaveränderung anzupassen beginnen.
    Notes: Summary Data from a standardized mist-netting programme (the so-called "MRI-Programm") were used to assess the timing of breeding in Reed Warblers (Acrocephalus scirpaceus) at Lake Constance (South Germany). The number of Reed Warblers fledged early in the season increased significantly between 1976 and 1997 (rs=0.614; p〈0.01). This is most probably due to a long-term increase in spring temperature as suggested by the high correlation between the number of early-fledged Reed Warblers and mean temperature in the first half of May (rs=0.416; p〈0.01). While in the period from 1961 to 1980 the mean date of spring arrival was on the 26th of April, it shifted towards the 19th April from 1981 to 1997. However, the number of early-fledged Reed Warblers and the date of spring arrival are not significantly correlated. The lack of a significant correlation may be due to a number of reasons, for instance early arriving birds do not belong to the local population or there could be an observational bias among years. Yet, the most likely explanation is that the timing of breeding in Reed Warblers is triggered by vegetation growth and food supply rather than by the timing of spring arrival. There is growing body of evidence from this and other studies that the recent climate change is responsible for the shift in both the timing of migration and breeding in birds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02462091
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  • 2
    Electronic Resource
    Electronic Resource
    Response of five insect herbivores to multiple allelochemicals under fluctuating temperatures (1998)
    Springer
    Entomologia experimentalis et applicata 88 (1998), S. 81-96 
    Details
    ISSN: 1570-7458
    Keywords: chlorogenic acid ; Heliothis virescens ; Manduca sexta ; Pseudoplusia includens ; rutin ; Spodoptera frugiperda ; temperature ; tomatine ; Trichoplusia ni
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of the combined effects of allelochemicals on insect herbivores is useful because there may be adverse additive or even synergistic effects. Analysis of the simultaneous effects of temperature and alleochemicals is also necessary because these factors may interact. We examined the effects of three allelochemicals found in tomato (chlorogenic acid, rutin and tomatine) and thermal regime (21:10 °C and 26:15 °C, representing spring and summer respectively) on five insect herbivores (a Solanaceae specialist, Manduca sexta, and the polyphagous Heliothis virescens, Pseudoplusia includens, Spodoptera frugiperda and Trichoplusia ni). There were allelochemical interactions and thermal regime-allelochemical interactions for all species, and so the patterns were complex. In some cases, paired allelochemicals or the combination of three allelochemicals showed adverse additive effects on insect performance. But that was not always the case, and there were only a few examples of synergism. Negative effects of the allelochemicals were sometimes, but not always, damped by the cooler thermal regime. Comparing the growth rates of the five species in this study with those of a previous study (a total of seven species) revealed five patterns. For two of three pairs of closely-related species, the paired species had distinctly different patterns. For example, for H. virescens, tomatine prevented development and chlorogenic acid slowed growth, whereas for Helicoverpa zea, tomatine just slowed growth and the phenolics had little effect. The specialist Manduca sexta had a pattern that was midway between patterns of the generalists; it was not the most tolerant of the allelochemicals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003290510565
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  • 3
    Electronic Resource
    Electronic Resource
    Non-reciprocal cross-incompatibility in Trichogramma deion (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 481-489 
    Details
    ISSN: 1570-7458
    Keywords: reproductive compatibility ; hybrid inviability ; temperature ; Trichogramma ; biological control ; Hymenoptera ; Trichogrammatidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In non-reciprocal cross-incompatibility (NRCI), the crossing of a female of a strain A with a male of a strain B results in hybrid offspring, whereas the reciprocal cross produces few or no hybrids. Only females are of hybrid origin in Hymenoptera because they arise from fertilized eggs; males arise from unfertilized (haploid) eggs. Crosses between many strains of Trichogramma deion showed some degree of NRCI. Crosses between a T. deion culture collected in Seven Pines, California (SVP) with one from Marysville, California (MRY) showed an extreme form of NRCI in which practically no female offspring was produced when MRY females were crossed with SVP males. The reciprocal cross produced a close to normal proportion of female and male offspring. Detailed studied of this cross indicated that 1) the female offspring produced in the compatible interstrain cross were not the result of parthenogenesis but were true hybrids, 2) the incompatible interstrain cross did not produce female offspring because fertilized eggs died during development, 3) the death of these eggs could not be prevented by either antibiotic or temperature treatment, 4) cytoplasmically inherited factors causing NRCI could be discounted because backcrossed females with the genome of MRY and the cytoplasm of SVP, exhibit the NRCI relationship characteristic of their genome. Therefore the NRCI between these strains appears to be caused by a modification coded for by the nuclear genes of MRY that results in incompatibility when SVP sperm fertilizes MRY eggs. In addition the level of incompatibility in crosses between the SVP females and MRY males is temperature sensitive, the higher the rearing temperature the lower the level of compatibility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00343472
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  • 4
    Electronic Resource
    Electronic Resource
    Effects of vegetation and weather on trap catches of Glossina fuscipes fuscipes near Lake Victoria, Kenya (1997)
    Springer
    Entomologia experimentalis et applicata 85 (1997), S. 231-236 
    Details
    ISSN: 1570-7458
    Keywords: Glossina fuscipes fuscipes ; vegetation ; biconical trap ; temperature ; light ; relative humidity ; monitor lizard
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Glossina fuscipes fuscipes Newstead was sampled in isolated thickets and forest patches near Lake Victoria, Kenya using unbaited biconical traps, between March 1992 and June 1993. Traps set at 1 m from the forest edge caught 3.3 times as many males and 5 times as many females as those set inside or 10 m away. The corresponding figures at 1 m from the edge of thicket were about 1.43 and 1.64 times, respectively. Hourly catches of males and females were positively correlated with temperature, light intensity and host (monitor lizard) prevalence, and negatively correlated with relative humidity. Light intensity and temperature were the most important variables affecting the catches of each sex. The results are discussed in relation to control and monitoring of G. f. fuscipes using traps.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003179128955
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  • 5
    Electronic Resource
    Electronic Resource
    Environmental effects on cytoplasmic incompatibility and bacterial load in Wolbachia-infected Drosophila simulans (1998)
    Springer
    Entomologia experimentalis et applicata 86 (1998), S. 13-24 
    Details
    ISSN: 1570-7458
    Keywords: Drosophila ; cytoplasmic incompatibility ; Wolbachia ; temperature ; antibiotics ; density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of high temperatures, antibiotics, nutrition and larval density on cytoplasmic incompatibility caused by a Wolbachia infection were investigated in Drosophila simulans. Exposure of larvae from an infected stock to moderate doses of tetracycline led to complete incompatibility when treated females were crossed to infected males; the same doses only caused a partial restoration of compatibility when treated males were crossed to uninfected females. In crosses with treated females, there was a strong correlation between dose effects on hatch rates and infection levels in embryos produced by these females. Ageing and rearing males at a high temperature led to increased compatibility. However, exposing infected females to a high temperature did not influence their compatibility with infected males. Male temperature effects depended on conditions experienced at the larval stage but not the pupal stage. Exposure to 25 °C reduced the density of Wolbachia in embryos compared with a 19 °C treatment. Low levels of nutrition led to increased compatibility, but no effect of larval crowding was detected. These findings show the ways environmental factors can influence the expression of cytoplasmic incompatibility and suggest that environmental effects may be mediated by bacterial density.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003043814761
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  • 6
    Electronic Resource
    Electronic Resource
    Different responses to temperature in three closely-related sympatric cereal aphids (1998)
    Springer
    Entomologia experimentalis et applicata 86 (1998), S. 49-58 
    Details
    ISSN: 1570-7458
    Keywords: aphids ; Sitobion ; population ; chromosomal race ; mean relative growth rate ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chromosomal races of the aphid Sitobion miscanthi (Takahashi), and a closely-related species S. near fragariae (Aphididae: Macrosiphini) show seasonal differences in abundance in eastern Australia. We tested whether this was related to differences in temperature response, using controlled laboratory conditions. Intrinsic rate of increase, rm, mean relative growth rate (MRGR), and longevity were compared at different temperatures, and rates of increase were compared outdoors under ambient conditions. Using laboratory data, we simulated population growth, for comparison with outdoor populations. There were significant differences in responses to temperature regimes both in the laboratory and outdoors. Outdoor populations fell well short of predicted sizes. S. miscanthi (2n=18) showed evidence of adaptation to warmer conditions. In contrast, S. nr fragariae showed consistent evidence for adaptation to cooler conditions, and S. miscanthi 2n=17 was approximately intermediate. These data indicate that temperature responses are important in the ecology, evolution and pest status of S. miscanthi clones and S. nr fragariae in Australia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003102927699
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  • 7
    Electronic Resource
    Electronic Resource
    Survival of Bemisia tabaci adults under different climatic conditions (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 511-519 
    Details
    ISSN: 1570-7458
    Keywords: sweet potato whitefly ; temperature ; relative humidity ; migration and dispersal ; virus transmission ; Homoptera ; Aleyrodidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ability of the sweet potato whitefly, Bemisia tabaci Gennad., to survive a range of environmental conditions was investigated in the laboratory. The range of temperature and humidity investigated corresponds to the normal climatic range during B. tabaci's summer migration in Israel. Adult whiteflies confined to small test cages were exposed to combinations of temperature (25, 30, 35, and 41 °C) and relative humidity (20, 50, 80, and 100%) for periods of 2, 4, or 6 h. A logistic regression model describing the four-dimensional surface defining percent survival as a function of time, temperature, and humidity was developed. Using stepwise regression to exclude non-significant terms, the linear predictor included temperature, and the products of temperature and time, and humidity and time. The model accounted for 75% of the variance. A reparameterization of the fitted regression model suggests that survival potential is conditioned by temperature conditions prevailing during the previous 10 h. Whitefly survival after 2 h exposure ranged from ≈ 90% survival at 20°C and 100% RH, to 〈2% survival at 41°C and 20% r.h.. No whiteflies survived more than 2 h exposure at these latter extremes of temperature and humidity. Survival rates decreased slightly after experimental whiteflies were kept in a cage with food a further 20 h at 25±2°C, 55±5% r.h. Investigations of the effects of hunger and virus infection, showed that both increased mortality.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00343476
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  • 8
    Electronic Resource
    Electronic Resource
    Choosing a place to grow. Importance of within-plant abiotic microenvironment for Yponomeuta mahalebella (1997)
    Springer
    Entomologia experimentalis et applicata 83 (1997), S. 171-180 
    Details
    ISSN: 1570-7458
    Keywords: within-plant heterogeneity ; tent caterpillars ; temperature ; circular statistics ; Lepidoptera ; Yponomeutidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The caterpillars of Yponomeuta mahalebella Latr. (Lepidoptera: Yponomeutidae) are monophagous on Prunus mahaleb (Rosaceae) leaves. Adult females deposit eggs in batches, which determines larval gregarious behaviour. Coupled with gregarious behaviour, caterpillars spin silk tents within they will feed until pupation. Distribution of tents in the field, their effect in microenvironmental larval growth conditions and the consequences for adult body mass and survival of larvae were studied. There was a significant trend for tents to be placed with a southward orientation. Within-plant tent orientation was related to the sun-shade pattern experienced, which was the main effect determining thermal differences between the inside and the outside of the tent. These differences appeared to affect larval growth and survival, with higher survival of larvae and heavier adults emerging from tents oriented to the south-east and east, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002908817924
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  • 9
    Electronic Resource
    Electronic Resource
    Life history ofAphis gossypii on cucumber: influence of temperature, host plant and parasitism (1995)
    Springer
    Entomologia experimentalis et applicata 76 (1995), S. 121-131 
    Details
    ISSN: 1570-7458
    Keywords: Aphis gossypii ; life history ; cucumber ; temperature ; partial resistance ; biological control ; Aphidius colemani
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Life table data forAphis gossypii Glover (Homoptera: Aphididae), an important pest in glasshouse cucumber crops, were studied at 20, 25 and 30°C on two cucumber cultivars (Cucumis sativus L.) in controlled climate cabinets. The development time on the cucumber cv. ‘Sporu’ ranged from 4.8 days at 20°C to 3.2 days at 30°C. Immature mortality was approximately 20% and did not differ between temperatures. Most mortality occurred during the first instar. Reproduction periods did not differ among temperatures, but at 25 and 30°C more nymphs were produced (65.9 and 69.8 nymphs/♀, respectively) than at 20°C (59,9 nymphs/♀) because of a higher daily reproduction. Intrinsic rate of increase was greatest at 25°C (r m =0.556 day−1). At 20 and 30°C the intrinsic rate of increase was 0.426 and 0.510, respectively. On cv. ‘Aramon’, the development time ofA. gossypii was approximately 20% longer at all temperatures. Immature mortality did not differ between the two cultivars. The intrinsic rate of increase on cv. ‘Aramon’ was 15% smaller than on cv. ‘Sporu’. The use of cucumber cultivars partially resistant to aphids is discussed in relation to biological control of cotton aphid in glasshouses. Development time and immature mortality on leaves of the middle and upper leaf layer of glasshouse grown cucumber plants (cv. ‘Aramon’) were comparable to development in the controlled climate cabinets. On the lower leaves immature mortality was much higher (approximately 82%) than on leaves of the middle (24.0%) and upper leaf layer (24.5%). Reproduction was less on the lower leaf layer (45.9, 70.5 and 70.1 nymphs/♀ on leaves of the lower, middle and upper leaf layer, respectively). Aphids, successfully parasitized byAphidius colemani Viereck (Hymenoptera: Braconidae) only reproduced when they were parasitized after the third instar. Fecundity was 0.1 to 0.9 and 10.5 to 13.3 nymphs/♀ for aphids parasitized in the fourth instar or as adults, respectively. Reproduction of aphids that were stung but survived the attack was lower than for aphids not stung. Average longevity of these aphids was equal to the longevity of aphids not stung byA. colemani.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02383210
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  • 10
    Electronic Resource
    Electronic Resource
    Flight behavior of pre- and postdiapause apple blossom weevils in relation to ambient temperature (1998)
    Springer
    Entomologia experimentalis et applicata 88 (1998), S. 97-99 
    Details
    ISSN: 1570-7458
    Keywords: Coleoptera ; Curculionidae ; Anthonomus pomorum ; temperature ; diapause ; flight behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003269621622
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  • 11
    Electronic Resource
    Electronic Resource
    Magnetometric Method of Investigating Fire Sites (1997)
    Springer
    Fire technology 33 (1997), S. 195-213 
    Details
    ISSN: 1572-8099
    Keywords: point of fire origin ; fire ; temperature ; plaster ; concrete ; thermal ; damage ; biotite ; magnetization ; susceptibility ; N-rays ; P-rays ; X-rays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying
    Notes: Abstract Magnetic susceptibility (MS) measurements were taken to determine fire exposure patterns on noncombustible structural members and compartment boundaries. These measurements led on-site fire investigators to the cause of combustion and the point of fire origin. Thermal damage to walls and to ceilings was estimated quantitatively by measuring MS and two-valent Fe ion concentration in building materials such as concrete and plaster. It was shown that these effects are preconditioned by thermochemical transformations in the mineral biotite, which is present as a component of common building materials. For the first time, the effect of a substantial and irreversible increase in biotite magnetization exposed to powerful flows of N- and P-rays was found. It is hoped that the presence of this “magnetic trace” of radiation in building materials will allow investigators to use the magnetometric method at nuclear power plant catastrophes such as that in Chernobyl. It was also shown that the influence of N- and P-rays on biotite's magnetic properties greatly differs from the thermal influence on biotite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1015363605037
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  • 12
    Electronic Resource
    Electronic Resource
    Effects of temperature and photoperiod on development and oviposition of the legume flower thrips, Megalurothrips sjostedti (1999)
    Springer
    Entomologia experimentalis et applicata 93 (1999), S. 149-155 
    Details
    ISSN: 1570-7458
    Keywords: Megalurothrips sjostedti ; temperature ; photoperiod ; development ; pre-oviposition ; oviposition ; diapause
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Megalurothrips sjostedti is an economic pest of cowpea in tropical Africa. There are no reports of the effect of temperature on the development of the pest and no diapause has been detected. The developmental rate and reproduction of M. sjostedti was studied under six constant temperatures (14, 17, 20, 23, 26 and 29 °C ) and a photoperiod of L12:D12 in the laboratory. Total developmental time ranged from 33.1 days at 14 °C to 19.2 days at 26 °C. At 29 °C, all larvae died during hatching. Egg, larval and pupal stages required 94.3, 97.1 and 105.3 deg-days (DD) above a threshold of 8.2, 9.1 and 10.4 °C, respectively, to complete development. Total developmental cycle was completed at 163.9 DD above a threshold of 12.6 °C. Adult longevity was lowest (11.1 days) at 29 °C and highest (28.7 days) at 14 °C. At constant temperatures, the highest pre-oviposition period was observed at 29 °C under a photoperiod of L16:D8. Egg production also ceased at this temperature/photoperiod combination. Total fecundity was highest at 26 °C under L10:D14. At cyclical temperatures, pre-oviposition period was longest (12.4 days) at 20/29 °C under L16:D8. Total fecundity was highest (168.4 eggs) at 14/26 °C under L10:D14, and lowest (8.2 eggs) at 20/29 °C under L16:D8. The significance of these findings in the management of M. sjostedti is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003896919284
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  • 13
    Electronic Resource
    Electronic Resource
    Influence of temperature and vapor pressure deficit on the rate of predation by the predatory mite, Amblyseius cucumeris, on Frankliniella occidentalis (1996)
    Springer
    Entomologia experimentalis et applicata 78 (1996), S. 31-38 
    Details
    ISSN: 1570-7458
    Keywords: Amblyseius cucumeris ; Frankliniella occidentalis ; rate of predation ; temperature ; vapor pressure deficit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The influence of temperature and vapor pressure deficit (VPD) on the rate of predation by the predatory mite, Amblyseius cucumeris (Oudemans) on Frankliniella occidentalis (Pergande) was determined under controlled laboratory conditions. The survival of first instars of F. occidentalis was initially determined by themselves. Then the number of first instars that were killed by a single female adult of A. cucumeris was determined for the same density of thrips. The differences in the mortality between the two experiments were used to calculate the rate of predation by A. cucumeris under a range of temperature and VPD regimes. Rate of predation was expressed as the number of thrips killed per h to account for the different time periods that the trials lasted. A quadratic model was fitted to the data. At a constant temperature, the rate of predation decreased with increasing VPD for VPDs 〈-1.24 to 1.44 kPa. Above these VPDs, the rate of predation started to increase again. At a constant VPD, the rate of predation decreased slightly at the lower temperatures and increased at the higher temperatures. The rate of change was dependent upon the VPD. By using the quadratic model, the rate of predation can be predicted for the range of hygrothermal conditions that would be encountered in the greenhouse. The optimal condition for maximum rate of predation by A. cucumeris on first instars of F. occidentalis in the greenhouse is a VPD 〈- 0.75 kPa at the recommended production temperatures and VPDs (17 to 25°C and 〈- 1.5 kPa).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304454
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  • 14
    Electronic Resource
    Electronic Resource
    Diapause survival and post-diapause performance in bumblebee queens (Bombus terrestris) (1998)
    Springer
    Entomologia experimentalis et applicata 89 (1998), S. 207-214 
    Details
    ISSN: 1570-7458
    Keywords: Bombus terrestris ; bumblebees ; diapause ; survival ; preoviposition period ; weight ; temperature ; diapause duration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diapause survival and post-diapause performance (i.e., if a queen starts to lay eggs) of in total 2210 bumblebee queens (Bombus terrestris) were measured under different diapause regimes: 5 temperatures (−5, 0, 5, 10 and 15 °C) in combination with 5 durations of exposure (1, 2, 4, 6 and 8 months). The results show that weight at the start of diapause determines to a large extent whether a queen will be able to survive diapause. Queens with a wet weight below 0.6 g prior to diapause did not survive, but for those queens exceeding this threshold a higher pre- diapause weight did not increase their post-diapause performance. There was no effect of temperature on diapause survival; 76% of the variance in survival could be explained by the duration of the treatment. Neither temperature nor duration of exposure had an effect on post-diapause performance. The preoviposition period of the queens that laid eggs was also determined. The preoviposition period was affected by both temperature and duration of exposure: the preoviposition period decreased with decreasing temperature but also with increasing duration of the treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003595306816
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  • 15
    Electronic Resource
    Electronic Resource
    Temperature influence on cohort parameters and demographic characteristics of the two cowpea coreids Clavigralla tomentosicollis and C. shadabi (1996)
    Springer
    Entomologia experimentalis et applicata 78 (1996), S. 201-213 
    Details
    ISSN: 1570-7458
    Keywords: cowpea ; Vigna unguiculata ; pod sucking bugs ; age-specific life tables ; temperature ; insect development ; cohort statistics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Age-specific life tables of two important pests of cowpea, Vigna unguiculata (L.) Walp., the pod sucking bugs Clavigralla tomentosicollis Stål and C. shadabi Dolling (Heteroptera: Coreidae), were obtained from observations carried out at different temperatures. A biophysical model was found satisfactory to describe the temperature-response of developmental and mortality rates of egg and nymphal stages, with a peak developmental rate around 34°C in both species. The variability in development times was small and the experimental data did not permit any conclusion with regard to the Erlang probability density function. Survival of eggs and nymphs remained high between 20° and 30°C for both species. At temperatures above 34°C, C. tomentosicollis survivorship and fecundity was higher than that of C. shadabi, which in turn laid more eggs at temperatures between 20° and 30°C. Maximum fecundity is estimated to be at 29°C for C. tomentosicollis (99 eggs/female) and 26°C for C. shadabi (261 eggs/female). At 30°C, the intrinsic rate of increase reached a maximum in both species, 0.152 per day for C. tomentosicollis and 0.145 per day for C. shadabi, and remained high for C. tomentosicollis until 36°C. C. tomentosicollis performed significantly better on pigeonpea, Cajanus cajan Millsp., than on cowpea at higher temperatures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00187518
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  • 16
    Electronic Resource
    Electronic Resource
    Influence of temperature on bionomics of cotton aphid, Aphis gossypii, on cotton (1999)
    Springer
    Entomologia experimentalis et applicata 90 (1999), S. 25-35 
    Details
    ISSN: 1570-7458
    Keywords: Aphis gossypii ; development ; survival ; fecundity ; intrinsic rate of increase ; life table ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Life table parameters of Aphis gossypii Glover (Homoptera: Aphididae) on Gossypium hirsutum L. were determined at six temperatures (10, 15, 20, 25, 30, and 35 ± 0.5 °C) in the laboratory. Relationships of life table parameters with temperature were described with mathematical equations. Development was fastest at 30 °C, with a pre-larviposition period of 4.6 d. Survival to adult was greatest at 25 °C (81%). Fecundity was highest at 25 °C, with a total fecundity of 28.3 nymphs per female and a mean reproductive rate of 3.1 nymphs per female per day. Threshold temperatures for development in the first through fourth instar and the adult were 8.2, 8.0, 7.2, 6.2 and 7.9 °C, respectively. The durations of these stages, expressed as temperature sums above these thresholds, were 24.2, 23.7, 23.0, 25.5 and 168.8 degree-days (D°), respectively. A. gossypii achieved its maximum net reproductive number (24.4 nymphs per female) and greatest intrinsic rate of increase (0.386 d−1) at 25 °C. The high relative rate of population increase at 25 °C results in a daily population increase of 47% and a doubling time of only 1.8 d, illustrating the tremendous growth capacity of A. gossypii populations under favourable conditions. Compared to literature sources, our source of A. gossypii, fed on cotton, showed a comparatively great heat tolerance.
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    URL: http://dx.doi.org/10.1023/A:1003580413487
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    Reproduction of Diaeretiella rapae on Russian wheat aphid hosts at different temperatures (1997)
    Springer
    Entomologia experimentalis et applicata 82 (1997), S. 159-166 
    Details
    ISSN: 1570-7458
    Keywords: Diaeretiella rapae ; reproduction ; life-tables ; temperature ; Diuraphis noxia ; biological control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diaeretiella rapae (M'Intosh), a polyphagous and cosmopolitan parasite of aphids, was imported from China to the USA for biological control of Diuraphis noxia (Mordwilko), a pest of small grains. We studied several aspects of its biology on D. noxia hosts in the laboratory at 3 constant temperatures, 10.0, 21.1, and 26.7 °C. Females lived significantly longer than males at all 3 temperatures, and longevity was greatest at 10 °C, followed by 21.1 and 26.7 °C. Fecundity (= number of mummies) did not differ significantly among the 3 temperatures studied. The ovarian egg-load was 129.1 ± 9.3, and was significantly affected by the size of adult females. A pre-oviposition period ± 0.26 days) was detected at 10 °C, but not at the other temperatures. Pre-imaginal survivorship was similar among temperatures, while R o and T c decreased with temperature, and r m increased with temperature. The offspring sex ratio (proportion females) was lowest at 26.7 °C, and similar between 10.0 and 21.1 °C. In addition, the offspring sex ratio significantly declined with the age of the female parent. The suitability of D. rapae for colonization against D. noxia in North America is discussed in relation to its responses to temperature and the climate of its home range.
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    URL: http://dx.doi.org/10.1023/A:1002929500786
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  • 18
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    Effects of light availability on host plant chemistry and the consequences for behavior and growth of an insect herbivore (1997)
    Springer
    Entomologia experimentalis et applicata 82 (1997), S. 319-333 
    Details
    ISSN: 1570-7458
    Keywords: chlorogenic acid ; light availability ; Lycopersicon esculentum ; Manduca sexta ; protein ; rutin ; temperature ; tomatine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined how light availability influenced the defensive chemistry of tomato (Lycopersicon esculentum: Solanaceae). Tomato plants were grown either in full sunlight or under shade cloth rated at 73%. Leaves from plants grown in full sunlight were tougher, had higher concentrations of allelochemicals (chlorogenic acid, rutin and tomatine), and had less protein than leaves from plants grown in shade. We determined how these differences in host plant quality due to light availability affected the behavior and growth of a Solanaceae specialist, Manduca sexta. Both in the greenhouse and in the field, caterpillars on shade-grown plants grew heavier in a shorter amount of time than those on plants that had previously been grown in full sunlight. In contrast, the effects of previous light availability to plants on caterpillar behavior appeared to be minor. To further investigate how light availability to plants influenced herbivore growth, we examined the effects of leaf-powder diets made from tomato leaves of different ages (new, intermediate, or mature) grown in full sunlight or shade on caterpillar performance. Caterpillars fed diets made from plants grown in shade consumed less but grew faster than larvae fed diets made from tomato plants grown in full sunlight. Caterpillars fed diets made from new leaves grew larger in less time than caterpillars fed diets made from intermediate aged leaves. Caterpillars did not survive on the mature leaf powder diets. There were plant-light treatment by larval thermal regime interactions. For example, at 26:15 °C , plant-light treatment had no effect on stadium duration, but at 21:10 °C, stadium duration was prolonged with the full sunlight-new leaf diet compared with the shaded-new leaf diet. In a second diet experiment, we examined the interactive effects of protein and some tomato allelochemicals (rutin, chlorogenic acid and tomatine) on the performance of caterpillars. There were food quality by thermal regime interactions. For instance, at 26:15 °C , neither protein nor allelochemical concentration influenced stadium duration, whereas at 21:10 °C, stadium duration was prolonged with the low protein-high allelochemical diet, which simulated full sunlight leaves. In sum, light availability to plants affected defensive chemistry and protein concentration. The difference in food quality was great enough to influence the growth of a specialist insect herbivore, but the effects were temperature-dependent.
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    URL: http://dx.doi.org/10.1023/A:1002916325052
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    Photoperiodic induction of larval diapause and temperature-dependent termination in a wild multivoltine bruchid,Kytorhinus sharpianus (1995)
    Springer
    Entomologia experimentalis et applicata 75 (1995), S. 127-134 
    Details
    ISSN: 1570-7458
    Keywords: bruchid ; diapause ; photoperiod ; temperature ; multivoltinism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A wild bean weevil,Kytorhinus sharpianus Bridwell (Coleoptera: Bruchidae), has a multivoltine life cycle and enters a hibernal larval diapause at the fourth instar under a short daylength (Shimada & Ishihara, 1991). Here, we investigated their diapause incidence under different photoperiods at 24°C and 27°C. The critical photoperiods for diapause induction were 14.5 h at 24°C and 14 h at 27°C. The stages susceptible to diapause-inducing stimuli were estimated by transferring larvae of various instars from long days to short days and vice versa. Then we investigated the incidence of larval diapause. The sensitive stage was estimated to be from the third to early fourth instar. Though larval diapause, which was induced under a short daylength, was terminated only by increasing the daylength, the termination was more synchronized by an exposure to a low temperature followed by increasing temperature, irrespective of photoperiod.
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    URL: http://dx.doi.org/10.1007/BF02382171
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  • 20
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    Inter- and intra-population differences in the effects of temperature on postdiapause development of Delia radicum (1997)
    Springer
    Entomologia experimentalis et applicata 84 (1997), S. 255-265 
    Details
    ISSN: 1570-7458
    Keywords: Delia radicum ; postdiapause development ; temperature ; Diptera ; Anthomyiidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Canadian populations of D. radicum differ in their response to temperature during postdiapause development. Populations that are primarily of the early-emerging type ( $$\left( { \leqslant 256DD_{04} } \right)$$ ) (St-Jean, Quebec; London, Ontario) have high values for the parameters describing this response: % MathType!MTEF!2!1!+-% feaafiart1ev1aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn% hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr% 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9% vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x% fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaGaamOuamaaBa% aaleaacaWGTbaabeaakiabg2da9aaa!38F2!$$R_m = $$ 12.7–13.3; $$T_m = $$ 28.0–31.8 °C ; Tσ = 10.3–14.2 ( $$R_m $$ , the maximum developmental rate at the temperature, $$T_m $$ [ °C ] where the developmental rate is highest, and Tσ , the parameter which gives the shape of the truncated normal curve fitted to the data), a low degree-day requirement for emergence (160–232 $$DD_{04} $$ ), and may lack a developmental delay at temperatures above ca. 21 °C . Populations of the late-emerging type (Kildare, Prince Edward Island) have low parameter values ( $$ R_m = 2.5$$ , $$T_m = 19.3$$ °C ; Tσ = 6.4), high degree-day requirements (530 $$DD_{04} $$ ), and a developmental delay at high temperatures. The parameters for the early-emergers in the population from Winnipeg, Manitoba (74% early) were intermediate ( $$R_m = 9.1$$ , $$T_m = 27.1$$ °C , Tσ = 10.7, $$ DD_{04} = 246 $$ ), but resembled the early rather than the late type. This population varied from 31 to 90% early type over a 10-year period and the rate of postdiapause development at 20 °C was directly related to the percentage early. In the year with the most rapid development (90% early), development was significantly slower than in the populations from other locations with predominantly early populations, and the year with the slowest development (31% early) showed significantly faster development than that from Kildare, Prince Edward Island (100% late). Therefore the parameters for early and late types of development will not be accurate for use in mixed populations, and the parameters in mixed populations will change among years. Populations of D. radicum in North America and Europe (67 locations by years) varied from 0–100% early. At Winnipeg, the percentage early was directly related to the annual temperature accumulation ( $$DD_{05} $$ ) during the growing season. The calculation of developmental parameters for the early-emergers of mixed populations provides a more accurate basis for estimating the times of first emergence and the first peak of emergence than parameters based on the whole population. Since postdiapause developmental rates vary both among and annually within locations, developmental models should be designed to include such variations.
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    URL: http://dx.doi.org/10.1023/A:1003048502446
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    Spring behavioral patterns of the apple blossom weevil (1996)
    Springer
    Entomologia experimentalis et applicata 79 (1996), S. 9-17 
    Details
    ISSN: 1570-7458
    Keywords: Anthonomus pomorum ; temperature ; diel cycle ; Coleoptera ; Curculionidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Individual pairs of overwintered adult apple blossom weevils, Anthonomus pomorum (L.), confined with apple twigs under different ambient temperatures in the laboratory and on apple trees in the field, were observed through day and night for their spring activities. Flight behavior in relation to ambient temperature was also investigated under laboratory conditions using flight stands. Both sexes displayed predominantly nocturnal behavior patterns in both the laboratory and the field. Feeding, crawling, and mating activities increased following sunset in the field or onset of scotophase in the laboratory while resting occurred most frequently during daylight hours. Results of the laboratory experiments showed that temperature affected significantly the activity patterns. The diel pattern of activities became less distinctive at higher temperatures (above 15°C), and total activities in crawling, feeding, and mating were suppressed significantly at lower temperatures (below 5°C). Over 97% of the test weevils initiated take-off response from flight stands at 20°C within the 30 min trial period; however, flight initiation rarely occurred at temperatures 12°C or below. Overall, results of the laboratory and field experiments indicate that A. pomorum is a remarkably cold-adapted insect with ability to crawl, feed, and mate at a few degrees above freezing, a physiological attribute necessary for the exploitation of early stages of apple bud development in the cold early spring.
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    URL: http://dx.doi.org/10.1007/BF00333816
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    The relative performance of pheromone and light traps in monitoring the seasonal activity of both sexes of the eastern hemlock looper, Lambdina fiscellaria fiscellaria (1998)
    Springer
    Entomologia experimentalis et applicata 89 (1998), S. 87-98 
    Details
    ISSN: 1570-7458
    Keywords: seasonal trapping ; light traps ; pheromone traps ; trapping periodicity ; temperature ; reproductive state ; eastern hemlock looper
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The seasonal flight activity of both sexes of the eastern hemlock looper, Lambdina fiscellaria fiscellaria Guenée (Lepidoptera: Geometridae) was studied during two consecutive years in Quebec and Newfoundland, using light (L), pheromone (P) and combined light and pheromone (LP) traps. Moth density significantly affected the performance of the different traps, with P traps being more effective at low than high density. However, P trap catches decreased just prior to the onset of female captures, probably as a result of competition between traps and virgin calling females. Nearly all females caught in L and LP traps were already mated and even the first females caught had laid at least half of their egg complement. In Quebec under warm nights, the pattern of male activity occurred at different times, with peak P catches being later in the scotophase than those of L traps, but overall similar numbers of males were caught in both traps. In contrast, under cool nights, males were caught early in the night in both P and L traps, suggesting a strong competition effect between traps, although more males were caught in P than L traps overall. In Newfoundland, the pattern of male captures in L and P traps was similar at both high and low temperatures, so competition between trap types would always be high. Under these conditions P traps were more effective than L traps. Irrespective of the region, year or temperature, significantly more males were captured in LP, with the effect of L and P being additive. In both regions, females responded similarly to L and LP traps with peak activity occurring early in the night. Captures of females were lower than those of males under cool temperatures, suggesting that the temperature threshold for flight is higher for females. The use of L and P traps simultaneously and/or in combination is discussed in relation to integrated pest management programs and ecological considerations.
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    URL: http://dx.doi.org/10.1023/A:1003450432755
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    Influence of environmental conditions and parasitoid quality on field performance of Trichogramma minutum (1996)
    Springer
    Entomologia experimentalis et applicata 80 (1996), S. 461-468 
    Details
    ISSN: 1570-7458
    Keywords: Trichogramma minutum ; Choristoneura fumiferana ; Ephestia kuehniella ; biological control ; inundative release ; weather ; host acceptance ; realized fecundity ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of weather conditions and two parasitoid quality attributes, realized fecundity and host acceptance, were assessed on the field efficacy of mass-released Trichogramma minutum. Temperature was the most important single variable, explaining up to 75% of the variation in field parasitism. There were significant positive relationships between both the sum of the maximum temperatures and the number of degree-hours above a 15 °C threshold, accumulated in the three days following the release, and parasitism in the field. There was a significant negative relationship between the mean relative humidity and the odds of parasitism in the field. Quality parameters based on parasitoid biology were not effective for predicting field efficacy if poor weather conditions persisted after a release. If weather conditions were ‘good’ (i.e. accumulated maximum temperatures above 62 °C, in the 3 days following the release), then parameters such as release rate and fecundity in the lab were useful predictors of field performance. There was no relationship between host acceptance measured in the lab and field parasitism. Given the importance of field temperatures for field performance, selection for cold tolerance of T. minutum would be desirable.
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    URL: http://dx.doi.org/10.1007/BF00343469
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    The smaller fruit tortrix, Grapholita lobarzewskii: predicting the phenology of adult emergence (1999)
    Springer
    Entomologia experimentalis et applicata 93 (1999), S. 297-302 
    Details
    ISSN: 1570-7458
    Keywords: smaller fruit tortrix ; Grapholita lobarzewskii ; phenology ; post-diapause development ; temperature ; thermal threshold ; thermal constant ; forecasting ; modelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The smaller fruit tortrix, Grapholita lobarzewskii Nowicki, has become a major pest in the recent past in apple orchards north of the Alps. Nevertheless little is known about the biology and the behaviour of this species. The effect of temperature on post-diapause development and survival was studied under controlled conditions in order to establish a basis for the forecasting of adult emergence. Survival was found to be highest at 17 °C and lowest at 11.8 °C. The sex ratio did not differ significantly from 1:1. Based on a linear relationship between temperature and developmental rates, thermal thresholds of 9.6 °C and 9.8 °C were determined for females and males respectively. The thermal constants, i.e., the mean developmental times in physiological time units, were found to be 342 day-degrees for females and 317 day-degrees for males, suggesting a slight protandry. Based on the mean and the variance of the developmental times, a simple temperature-driven phenology model was built using a time-varying distributed delay. The model was validated by visually comparing the predictions with independent observations on adult emergence, and by calculating the temporal deviations of the predictions. In 4 out of 5 years the mean error was less than 3 days. The model was therefore found to give reliable forecasts of the emergence of G. lobarzewskii and can be used to determine the optimal time for the exposure of pheromone traps, the application of pheromone dispensers for mating disruption, and for the timing of insecticide applications.
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    URL: http://dx.doi.org/10.1023/A:1003905026249
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    Temperature Effects on Soil Mineralization of Polylactic Acid Plastic in Laboratory Respirometers (1999)
    Springer
    Journal of polymers and the environment 7 (1999), S. 101-108 
    Details
    ISSN: 1572-8900
    Keywords: Polylactic acid ; temperature ; respirometers ; soil degradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A respirometric system was used to analyze the biodegradation of high molecular weight (120,000 to 200,000 g mol−1) polylactic acid (PLA) plastic films in soil under laboratory conditions. The respirometric system consisted of air-conditioning pretraps, a soil reactor, and a carbon dioxide (CO2) posttrap. A 200-g homogeneous soil mixture of all-purpose potting soil : manure soil : sand [1 : 1 : 1 (w/w)] and 1.5 g of PLA plastic films in 1 × 1-cm2 squares was added to each bottle. The respirometers were placed in a 28, 40, or 55°C water bath for 182 days. Treatments (three replicates) included native corn starch (positive control), polyethylene (Glad Cling Wrap; negative control), and three PLA films: Ca-I (Cargill Dow Polymers LLC, monolayer), GII (Cargill Dow Polymers LLC, Generation II), and Ch-I (Chronopol; monolayer). The degree of polymer mineralization was indicated by the cumulative CO2 liberated from each respirometer. The initial average mineralization rate and total percentage mineralized of the PLA plastic films at 28, 40, and 55°C was 24.3, 41.5, and 76.9 mg/day with a 27, 45, and 70% carbon loss, respectively. No decrease in soil pH was observed after 182 days of mineralization. Hence, increase in soil temperature drastically enhanced the biodegradation of PLA plastic films in soil under laboratory conditions (P 〈 0.0001).
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    URL: http://dx.doi.org/10.1023/A:1021812418325
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    Effect of temperature on performanceof LaNi4.76Sn0.24 metal hydride electrode’ (1997)
    Springer
    Journal of applied electrochemistry 27 (1997), S. 1328-1332 
    Details
    ISSN: 1572-8838
    Keywords: metal hydride ; capacity ; temperature ; performance ; enthalpy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract The effect of temperature on the performance of a LaNi4.76Sn0.24 metal hydride electrode was investigated in the temperature range of 0 to 50°C. The electrode showed a maximum discharge capacity at 25°C. The total resistance increases with a decrease of temperature from 50°C to 0°C. The apparent activation enthalpies at different states of charge were determined by evaluating the polarization resistance at different temperatures. It was found that the apparent activation enthalpy is an indicator of the relative reaction rate of the charge-transfer reaction and hydrogen absorption.
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    URL: http://dx.doi.org/10.1023/A:1018460709968
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    Effects of Temperature and Relative Humidity on Polylactic Acid Plastic Degradation (1999)
    Springer
    Journal of polymers and the environment 7 (1999), S. 83-92 
    Details
    ISSN: 1572-8900
    Keywords: Polylactic acid ; temperature ; relative humidity ; degradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Three high molecular weight (120,000 to 200,000 g mol−1) polylactic acid (PLA) plastic films from Chronopol (Ch-I) and Cargill Dow Polymers (GII and Ca-I) were analyzed for their degradation under various temperature and relative humidity (RH) conditions. Two sets of plastic films, each containing 11 samples, were randomly hung in a temperature/humidity-controlled chamber by means of plastic-coated paper clips. The tested conditions were 28, 40, and 55°C at 50 and 100% RH, respectively, and 55°C at 10% RH. The three tested PLA films started to lose their tensile properties when their weight-average molecular weight (M w) was in the range of 50,000 to 75,000 g mol−1. The average degradation rate of Ch-I, GII, and Ca-I was 28,931, 27,361, and 63,025 M w/week, respectively. Hence, GII had a faster degradation rate than Ch-I and Ca-I under all tested conditions. The degradation rate of PLA plastics was enhanced by the increase in temperature and relative humidity. This trend was observed in all three PLA plastics (Ca-I, GII, and Ch-I). Of the three tested films, Ch-I was the first to lose its mechanical properties, whereas Ca-I demonstrated the slowest loss, with mechanical properties under all tested conditions.
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    URL: http://dx.doi.org/10.1023/A:1021808317416
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    Electrochemical synthesis of cupric oxide powder Part II: Process conditions (1999)
    Springer
    Journal of applied electrochemistry 29 (1999), S. 81-85 
    Details
    ISSN: 1572-8838
    Keywords: cupric oxide ; powder ; current density ; temperature ; sodium sulfate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Notes: Abstract Electrosynthesis of cupric oxide powder was carried out on a laboratory scale in an electrochemical cell under various experimental conditions. The electrolysis was appraised in terms of the particle size of the cupric oxide product and the anodic dissolution of the copper electrode. Using a previously determined pH value of 7.50, the other optimum electrolysis operating conditions established were a current density of 4000Am−2, temperature of 353K, and Na2SO4 concentration of 0.5m. The optimum values of current efficiency, cell voltage and specific energy consumption for the electrochemical synthesis of cupric oxide powder were determined.
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    URL: http://dx.doi.org/10.1023/A:1003464914657
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    Sulfate solubility algorithms for CaSO4–NaCl–H2O and Na2SO4–NaCl–H2O in chlor alkali brine at 25, 65, 75 and 85 ∘C (1999)
    Springer
    Journal of applied electrochemistry 29 (1999), S. 525-528 
    Details
    ISSN: 1572-8838
    Keywords: brine ; chlor-alkali cells ; solubility ; sulfate ions ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026405806916
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    Excess volumes of binary mixtures of ethylbenzene with octanol, nonanol and dodecanol from 50 to 100°C and 0.1 to 7.5 MPa (1995)
    Springer
    Journal of solution chemistry 24 (1995), S. 153-170 
    Details
    ISSN: 1572-8927
    Keywords: Densities ; excess volume ; pressure ; temperature ; ethylbenzene ; octanol ; nonanol ; dodecanol ; order creation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The excess volume V E of binary mixtures of octanol, nonanol and dodecanol in ethylbenzene have been calculated from the densities measured with a vibrating tube densimeter at temperatures from 50 to 100 °C and at pressures from 0.1 to 7.5 MPa. The values of V E are positive for all the three mixtures in the complete temperature, pressure and mole fraction ranges studied. The maxima in V E is observed at 0.4 mole fraction of alkanol. The results are discussed in terms of specific interactions present in the binary mixtures. The second order thermodynamic quantities (∂V E /∂T)p,(∂V E /∂P)T and (∂V E /∂P)T which have been derived from the effect of temperature and pressure on V E , indicate an overall net creation of order in the binary mixtures of ethylbenzene with higher homologues of alkanols.
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    URL: http://dx.doi.org/10.1007/BF00972839
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    Electrical conductances of aqueous sodium trifluoromethanesulfonate from 0 to 450°C and pressures to 250 MPa (1995)
    Springer
    Journal of solution chemistry 24 (1995), S. 753-769 
    Details
    ISSN: 1572-8927
    Keywords: Conductivity ; sodium trifluoromethanesulfonate ; ion association ; temperature ; pressure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The electrical conductances of dilute (0.001 to 0.1 mol-kg−1) aqueous sodium trifluoromethanesulfonate (NaCF3SO3) solutions have been measured from 0 to 450°C and pressures to 250 MPa. The limiting molar conductance $$\Lambda _0 $$ increases with increasing temperature from 0 to 300°C and decreasing density from 0.8 to 0.3 g-cm−3. Above 300°C, $$\Lambda _0 $$ is nearly temperature independent, but increases linearly with decreasing density. The logarithm of the molal association constant of NaCF3SO3 calculated at temperatures from 372 to 450°C is represented as a function of temperature (Kelvin) and density of water (g-cm−3) by $$\log K_m = 0.888 - 330.4/T - (12.83 - 5349/T)\log \rho _w $$ The relative strengths of NaCF3SO3 and NaCl are similar within the accuracy of the current measurements over the limited range of temperature and pressure that could be investigated here.
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    Low ambient temperature decreases cadmium accumulation in the liver and kidneys of the bank vole (Clethrionomys glareolus) (1996)
    Springer
    BioMetals 9 (1996), S. 363-369 
    Details
    ISSN: 1572-8773
    Keywords: cadmium ; copper ; iron ; photoperiod ; temperature ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The importance of photoperiod and ambient temperature on the accumulation of cadmium in the liver and kidneys of bank voles was determined in the present study. Males and females, aged 1 month, were given 3.0 μg Cd ml−1 drinking water and divided into four groups according to photoperiod (16 h light/8 h dark and 8 h light/16 h dark) and ambient temperature (20 or 5°C); liver and kidneys were removed for cadmium as well as copper, iron and zinc analyses at the end of 6 weeks. Bank voles exposed to 5°C in both photoperiods consumed approximately 30% less water containing cadmium than those kept at 20°C. However, the total accumulation of cadmium in the liver and kidneys of males and females exposed to the low temperatures was 4.3–4.8 and 2.2–3.3 times less than that in animals maintained at room temperature in the long and short photoperiod, respectively. Simultaneously, the low temperature brought about an increase in the copper concentrations in the liver (12–43%) and kidneys (47–78%), giving rise to an inverse correlation between the cadmium accumulation and the tissue copper concentration. In contrast to cadmium and copper, the concentrations of iron and zinc were affected primarily by photoperiod. These findings indicate that ambient temperature is an important determinant of cadmium retention in the bank vole. It appears that low temperature decreases tissue cadmium accumulation not only by reducing cadmium intake but also through changes in copper metabolism.
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    Voltammetric Analysis of Iodide and Diffusion Coefficients Between 25 and 85°C (1998)
    Springer
    Journal of solution chemistry 27 (1998), S. 373-393 
    Details
    ISSN: 1572-8927
    Keywords: Iodide ; voltammetry ; diffusion coefficients ; temperature ; Stokes–Einstein equation ; solvation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The oxidation wave of iodide in 0.075 mol-L−1 H2SO4 was analyzed at 25, 40, 55, 70, and 85°C. The reversibility of the I2/I− system was checked using logarithmic transforms, half-wave potentials, and by studying I −1 = f(ω−1/2). The limiting currents obtained enabled us to determine the diffusion coefficient of I− using Newman's equation. These experimental results were compared with Nernst's limiting values. The Stokes–Einstein equation is not verified. Hydration numbers for I− at different temperatures were established. An empirical equation is proposed to predict the evolution of diffusion coefficients in a sulfuric acid medium with temperature.
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    Ion association of dilute aqueous sodium hydroxide solutions to 600°C and 300 MPa by conductance measurements (1996)
    Springer
    Journal of solution chemistry 25 (1996), S. 711-729 
    Details
    ISSN: 1572-8927
    Keywords: Conductivity ; aqueous ; sodium hydroxide ; ion association ; temperature ; pressure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The limiting molar conductances Λ0 and ion association constants of dilute aqueous NaOH solutions (〈0.01 mol-kg−1) were determined by electrical conductance measurements at temperatures from 100 to 600°C and pressures up to 300 MPa. The limiting molar conductances of NaOH(aq) were found to increase with increasing temperature up to 300°C and with decreasing water density ρw. At temperatures ≥400°C, and densities between 0.6 to 0.8 g-cm−3, Λ0 is nearly temperature-independent but increases linearly with decreasing density, and then decreases at densities 〈0.6 g-cm−3. This phenomenon is largely due to the breakdown of the hydrogen-bonded, structure of water. The molal association constants K Am for NaOH( aq ) increase with increasing temperature and decreasing density. The logarithm of the molal association constant can be represented as a function of temperature (Kelvin) and the logarithm of the density of water by $$\begin{gathered} log K_{Am} = 2.477 - 951.53/T - (9.307 \hfill \\ - 3482.8/T)log \rho _{w } (25 - 600^\circ C) \hfill \\ \end{gathered} $$ which includes selected data taken from the literature, or by $$\begin{gathered} log K_{Am} = 1.648 - 370.31/T - (13.215 \hfill \\ - 6300.5/T)log \rho _{w } (400 - 600^\circ C) \hfill \\ \end{gathered} $$ which is based solely on results from the present study over this temperature range (and to 300 MPa) where the measurements are most precise.
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    Transglutaminase induction by various cell death and apoptosis pathways (1996)
    Springer
    Cellular and molecular life sciences 52 (1996), S. 942-949 
    Details
    ISSN: 1420-9071
    Keywords: Apoptosis ; transglutaminase ; signalling ; gene expression ; promoter elements ; retinoic acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Clarification of the molecular details of forms of natural cell death, including apoptosis, has become one of the most challenging issues of contemporary biomedical sciences. One of the effector elements of various cell death pathways is the covalent cross-linking of cellular proteins by transglutaminases. This review will discuss the accumulating data related to the induction and regulation of these enzymes, particularly of tissue type transglutaminase, in the molecular program of cell death. A wide range of signalling pathways can lead to the parallel induction of apoptosis and transglutaminase, providing a handle for better understanding the exact molecular interactions responsible for the mechanism of regulated cell death.
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    Analysis of gene function inDictyostelium (1995)
    Springer
    Cellular and molecular life sciences 51 (1995), S. 1116-1123 
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    ISSN: 1420-9071
    Keywords: Antisense RNA ; gene expression ; insertional mutagenesis ; physical mapping ; reporter genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Over the past ten years, powerful molecular genetic techniques have been developed to analyze gene function inDictyostelium. DNA-mediated transformation using a variety of selections and vectors has allowed the introduction of wild-type or modified genes that are under various forms of transcriptional control. Homologous recombination is efficient and can be used to modify the genome in precise ways. In addition, it is now possible to clone genes based on their mutant phenotype alone, either by insertional mutagenesis, or by screening antisense expression cDNA libraries. Finally, a nearly complete physical map of the genome is available and so genes are easily mapped by physical techniques. We discuss many of these advances within the context of major research problems presently under study.
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    URL: http://dx.doi.org/10.1007/BF01944729
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    Regulation of metallothionein gene expression in Cd- or Zn-adapted RK-13 cells (1995)
    Springer
    Cellular and molecular life sciences 51 (1995), S. 606-611 
    Details
    ISSN: 1420-9071
    Keywords: Metallothionein ; isometallothioneins ; gene expression ; rabbit kidney cell-line ; cadmium adaptation ; zinc adaptation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We explored the molecular genetics underlying the massive induction of isoMTs by Zn2+ or Cd2+ in metal tolerant rabbit kidney (RK-13) sub-line cells, using band shift assays and Southern blotting analysis. In sub-line cells accommodated to intermediate metal concentrations (100 μM Zn2+; 1–20 μM Cd2+) evidence suggested that the increase in the capacity for isoMT synthesis is brought about by an increased binding activity of the nuclear transcription factors MTF-1 and Sp1. Using quantitative band shift analysis with a mouse MRE-d oligonucleotide probe, the binding of both transcription factors was found to be enhanced two to three times over the binding activity measured in the unexposed parental RK-13 cells. Their increase in binding activity is probably the cause of the overexpression of MT genes and the development of metal tolerance in these cells. In cells tolerant to the highest concentrations of metal the analysis of Southern blot signals revealed MT gene amplification to be the most probable cause of the increased MT production. Thus, in cells of sub-lines growing in the presence of 350 μM Zn2+, two of the isoMT genes were coordinately triplicated and in cells tolerant to 150 μM Cd2+ one isoMT gene was amplified two-fold.
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    Nuclear calcium: a key regulator of gene expression (1998)
    Springer
    BioMetals 11 (1998), S. 345-358 
    Details
    ISSN: 1572-8773
    Keywords: calcium ; CREB ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Through the evolution of multicellular organisms, calcium has emerged as the preferred ion for intracel-lular signalling. It now occupies a pivotal role in many cell types and nowhere is it more important than in neurons, where it mediates both the relaying and long-term storage of information. The latter is a process that enables learning and memory to be formed and requires the activation of gene expression by calcium signals. Evidence from a number of diverse organisms shows that transcription mediated by the transcrip-tion factor CREB is critical for learning and memory. Here we review the features of CREB activation by calcium signals in mammalian cells. In contrast to other transcription factors, its regulation is dependent on an elevation of nuclear calcium concentration, potentially placing this spatially distinct pool of calcium as an important mediator of information storage.© Kluwer Academic Publishers
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    Thermoelastic contact problem of two rotating bodies with frictional heat generation in annular region (1998)
    Springer
    Archive of applied mechanics 68 (1998), S. 128-136 
    Details
    ISSN: 1432-0681
    Keywords: Key words frictional heating ; temperature ; contact pressure ; punch indentation ; thermoelasticity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary An axisymmetric contact problem with frictional heating is considered in which a parabolic annular punch is pressed into a plane surface and rotates about its axis of symmetry at constant speed. The problem is formulated in terms of one governing equation with unknown pressure. This equation is solved numerically. The change of the geometry of the contact region and pressure has been investigated.
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    Effect of time-dependent speed on frictional heat generation and wear in transient axisymmetrical contact of sliding (1997)
    Springer
    Archive of applied mechanics 67 (1997), S. 331-338 
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    ISSN: 1432-0681
    Keywords: Key words frictional heating ; wear ; contact pressure ; temperature ; brake system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Summary A transient contact problem with frictional heating and wear for two nonuniform sliding half-spaces is considered. One of the two half-spaces is assumed to be slightly curved to give a Hertzian initial pressure distribution: the other is a rigid nonconductor. Under the assumption that the contact pressure distribution could be described by Hertz formulas during all the process of interaction, the problem is formulated in terms of one integral equation of Volterra type with unknown radius of contact area. A numerical solution of this equation is obtained using a piecewise-constant presentation of an unknown function. The influence of operating parameters on the contact temperature and the radius of the contact area is studied.
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    Multiplex Titration RT-PCR: Rapid Determination of Gene Expression Patterns for a Large Number of Genes (1998)
    Springer
    Plant molecular biology reporter 16 (1998), S. 323-339 
    Details
    ISSN: 1572-9818
    Keywords: Aux/IAA genes ; gene expression ; gene families ; RT-PCR ; tomato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed an improved method for determination of gene expression levels with RT-PCR. The procedure is rapid and does not require extensive optimization or densitometric analysis. Since the detection of individual transcripts is PCR-based, small amounts of tissue samples are sufficient for the analysis of expression patterns in large gene families. Using this method, we were able to rapidly screen nine members of the Aux/IAA family of auxin- responsive genes and identify those genes which vary in message abundance in a tissue- and light-specific manner. While not offering the accuracy of conventional semi-quantitative or competitive RT-PCR, our method allows quick screening of large numbers of genes in a wide range of RNA samples with just a thermal cycler and standard gel analysis equipment.
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    Isolation of RNA and Protein from Guard Cells of Nicotiana glauca (1999)
    Springer
    Plant molecular biology reporter 17 (1999), S. 371-383 
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    ISSN: 1572-9818
    Keywords: epidermal peel ; extraction ; gene expression ; stomata ; tree tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Stomatal guard cells are critical for maintenance of plant homeostasis and represent an interesting cell type for studies of leaf cell differentiation and patterning. Here we describe techniques for the isolation of guard cell RNA and protein from blended epidermal peels of Nicotiana glauca. The RNA isolation procedure is a modification of the hot borate method, which is particularly well-suited for recalcitrant tissues. Protein was extracted by disrupting guard cell-enriched epidermis with a French® press. This system offers the following advantages: relatively high yield, low or no contamination by other cell types, fresh tissue as a source of RNA and protein rather than protoplasts, and a plant species that is readily transformable. These techniques will allow for cloning and analysis of genes expressed in guard cells, application of traditional biochemical techniques to guard cell proteins, as well as characterization of genetic manipulation of guard cell function in transgenic plants.
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    Assessment of freshwater diatoms as quantitative indicators of past climatic change in the Yukon and Northwest Territories, Canada (1995)
    Springer
    Journal of paleolimnology 13 (1995), S. 21-49 
    Details
    ISSN: 1573-0417
    Keywords: diatoms ; temperature ; climatic change ; paleoclimate proxies ; canonical correspondence analysis ; weighted-averaging ; Yukon ; Northwest Territories
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract We identified, enumerated, and interpreted the diatom assemblages preserved in the surface sediments of 59 lakes located between Whitehorse in the Yukon and Tuktoyaktuk in the Northwest Territories (Canada). The lakes are distributed along a latitudinal gradient that includes several ecoclimatic zones. It also spans large gradients in limnological variables. Thus, the study lakes are ideal for environmental calibration of modern diatom assemblages. Canonical correspondence analysis, with forward selection and Monte Carlo permutation tests, showed that maximum lake depth and summer surface-water temperature were the two environmental variables that accounted for most of the variance in the diatom data. The concentrations of sodium and calcium were also important explanatory variables. Using weighted-averaging regression and calibration techniques, we developed a predictive statistical model to infer lake surface-water temperature, and we evaluated the feasibility of using diatoms as paleoclimate proxies. This model may be used to derive paleotemperature inferences from fossil diatom assemblages at appropriate sites in the western Canadian Arctic.
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    Sedimentation patterns of diatoms in Lake Holzmaar, Germany - (on the transfer of climate signals to biogenic silica oxygen isotope proxies) (1999)
    Springer
    Journal of paleolimnology 21 (1999), S. 437-448 
    Details
    ISSN: 1573-0417
    Keywords: diatoms ; temperature ; sediment traps ; seasonal succession ; resuspension ; Lake Holzmaar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract The seasonal sedimentation pattern of diatom valves in Lake Holzmaar was investigated during 1995 by deploying sediment traps at three different lake depths. According to the sedimentation pattern, the major reproduction zone of diatoms was restricted to the upper 6 m of the water body. The population growth started late in April and blooms of Cyclotella cf. comensis Grun., which dominates the plankton diatoms, and Fragilaria crotonensis Kitton were collected in traps during June and September, and July, respectively. During summer, the seasonal sedimentation pattern of each taxon, as collected in the upper traps, was reflected in the concentrations in the lowest trap. However, in May and from September onwards, the community composition in the lowest trap and augmented trapping rates suggest both sediment focusing and resuspension of bottom sediments. The temperature signals as recorded by δ18O values of diatom valves should, therefore, reflect integrated temperatures between 0 and 6 m depth. However, temperatures during summer and autumn are expected to be accentuated in the sedimentary record since the isotopic signal is weighted by both the number and the weight-mass of the valves. During summer, the transfer of these signals by the sedimenting diatoms retains the information pattern recorded, while in spring and autumn/winter additional influxes caused by resuspension may somewhat alter those temperature informations. The proxy signals finally stored in the sediments, may, therefore, not precisely represent the successive temperatures currently recorded during 1995 within mid-lake.
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    The environmental history of a mountain lake (Lago Paione Superiore, Central Alps, Italy) for the last c. 100 years: a multidisciplinary, palaeolimnological study (1996)
    Springer
    Journal of paleolimnology 15 (1996), S. 245-264 
    Details
    ISSN: 1573-0417
    Keywords: palaeolimnology ; acidification ; diatoms ; chrysophytes ; pigments ; chironomids ; temperature ; Alps ; Italy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract A palaeoecological study of an oligotrophic alpine lake, Paione Superiore (Italy), provided a record of historical changes in water quality. Historical trends in lake acidification were reconstructed by means of calibration and regression equations from diatoms, chrysophycean scales and pigment ratios. The historical pH was inferred by using two different diatom calibration data sets, one specific to the alpine region. These pH trends, together with the record of sedimentary carbonaceous particles and chironomid remains, indicate a recent acidification of this low alkalinity lake. Concentration of total organic matter, organic carbon, nitrogen, biogenic silica (BSiO2), chlorophyll derivatives (CD), fucoxanthin, diatom cell concentration and number of chironomid head capsules increased during the last 2–3 decades. When expressed as accumulation rates, most of these parameters tended to decrease from the past century to c. 1950, then all except P increased to the present day. A marked increase in sedimentary nitrogen may be related to atmospheric pollution and to the general increases in output of N in Europe. High C/N ratios indicate a prevailing allochthonous source of organic matter. Finally, the increase in measured air temperature from the mid-1800's appeared to be related to lake water pH before industrialization: cold periods generally led to lower pH and vice-versa. The more recent phenomenon of anthropogenic acidification has apparently decoupled this climatic-water chemistry relationship.
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    Effect of environmental conditions and inoculum density on infection of guava fruits by Colletotrichum glososporioides (1997)
    Springer
    Mycopathologia 137 (1997), S. 165-172 
    Details
    ISSN: 1573-0832
    Keywords: Anthracnose ; guava ; Colletotrichum glososporioides ; temperature ; free moisture ; inoculum density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The influence of environmental factors (temperature and humidity), inoculum density on infection by Colletotrichum glososporioides and development of anthracnose lesions were determined on uninjured, sand-injured and punctured fruits. The optical temperature for severe infection was 30 °C, whereas the disease incidence was less at 20 and 35 °C. Inoculated guavas that received 1–60 h of continuous free moisture developed lesions, but the disease was minimal (0–7%) after 1–6 h free moisture. Infection rates of uninjured, sand-injured and punctured fruits receiving 60 h of free moisture were 34, 70 and 100%, respectively. Disease incidence increased as inoculum density increased from 101 to 106 conidia/ml. In field conditions, the development of anthracnose lesions was greater on punctured guavas than on uninjured or sand-injured ones, in both rainy and winter seasons. In general, the number of lesions was highest in sand-injured fruits, followed by punctured and uninjured fruits. In rainy season the number of lesions on injured and uninjured fruits was greater than similarly treated guavas in winter.
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    Effect of temperature on antagonistic and biocontrol potential of shape Trichoderma sp. on Sclerotium rolfsii (1997)
    Springer
    Mycopathologia 139 (1997), S. 151-155 
    Details
    ISSN: 1573-0832
    Keywords: Antagonism ; biocontrol ; ginger ; postharvest rot ; Sclerotium rolfsii ; Trichoderma ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Sclerotium rolfsii is a destructive soil-borne and postharvest plant pathogen. Use of the antagonistic fungus Trichoderma sp. has been earlier reported by us to successfully control this pathogen under postharvest conditions. In the present paper we report on the effects of temperature on the growth and biocontrol potential of Trichoderma sp. on S. rolfsii. Experimental results indicated that S. rolfsii and Trichoderma sp. have different temperature optima for growth: 30–35 °C for the pathogen and 25–30 °C for the antagonist. In dual culture, Trichoderma overgrew S. rolfsii at 25 °C and 30 °C, but at 35 °C and 37 °C, S. rolfsii overgrew the colony of Trichoderma. Trichoderma produced higher concentration of fungitoxic metabolites in broth culture at higher temperatures. In bioassays using ginger slices and whole rhizomes, it has been demonstrated that Trichoderma is not very effective in suppressing S. rolfsii at temperatures above 30 °C. In light of these results, possible mechanisms of biocontrol of S. rolfsii as a postharvest pathogen has been discussed. Storage temperature has been suggested as a critical factor in biocontrol of S. rolfsii.
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    Fungi associated with post-harvest rot of black plum (Vitex doniana) in Nigeria (1996)
    Springer
    Mycopathologia 136 (1996), S. 109-114 
    Details
    ISSN: 1573-0832
    Keywords: Fungi ; post-harvest rot ; relative humidity ; temperature ; Vitex doniana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The fungi associated with rot of Vitex doniana fruits (blackplum) were isolated and identified. Aspergillus niger, Botryodiplodia theobromae, Candida spp. Penicillium chrysogenum, Rhizopus stolonifer, Fusarium pallidoroseum F. oxysporum and Mucor mucedo were the primary rot causing fungi in contrast to Cladosporium herbarum and Mucor circinelloides which were just present as secondary colonizers. The rot fungi penetrated mainly through wounds and bruises on the surface of fruits. Mature green fruits were less susceptible to infection than half ripe and fully ripened red fruits. Optimum rot by pathogenic isolates occurred at 25–30 °C and relative humidity 72.5–100%. The results of investigation of influence of storage temperatures and relative humidity on the quality of uninoculated healthy fruits are presented and discussed.
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    Environment factors influence in vitro interspecific interactions between A. ochraceus and other maize spoilage fungi, growth and ochratoxin production (1999)
    Springer
    Mycopathologia 146 (1999), S. 43-47 
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    ISSN: 1573-0832
    Keywords: interspecific interactions ; ochratoxin ; Aspergillus ochraceus ; spoilage fungi ; water relations ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The effect of water availability (water activity,aw; 0.995–0.90) and temperature (18–30 °) on in vitro interactions between an ochratoxin producing strain of Aspergillus ochraceus and six other spoilage fungi was assessed in dual culture experiments on a maize meal-based agar medium. Inprimary resource capture of nutrient substrate, A. ochraceus was dominant against many of the interacting species, being able to overgrow and replace A. candidus, and sometimes A. flavus and the Eurotium spp. regardless of aw or temperature. However, with freely available water (0.995 aw) A. alternata and A. niger were dominant, with mutual antagonism between A. ochraceus and A. flavus at 25–30 °C. In the driest conditions tested (0.90 aw) there was also mutual antagonism between A. ochraceus and the two Eurotium spp. Overall, under allconditions tested the Index of Dominance for A. ochraceus was much higher than for other competing species combined suggesting that A. ochraceus wasa good competitive colonist able to replace a numberof other species. However, the growth rate ofA. ochraceus was modified and decreased by the interaction with competitors. Interaction between A. ochraceus and species such as A. alternata (18°C/0.995) and Eurotium spp. (0.995–0.95 and 25–30 °C) resulted in a significant stimulation of ochratoxin production. Theresults are discussed in relation to the effect that environmental factors have on the possible competitiveness of A. ochraceus in the maizegrain ecosystem and the role of ochratoxin in nicheexclusion of competitors.
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    Phosphorus supplying capacity of heavily fertilized soils II. Dry matter yield of successive crops and phosphorus uptake at different temperatures (1996)
    Springer
    Nutrient cycling in agroecosystems 47 (1996), S. 123-134 
    Details
    ISSN: 1573-0867
    Keywords: heavily fertilized soils ; P supplying capacity ; P uptake ; residual P ; soil test methods ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nine heavily fertilized soils were collected from southern and central Norway. A greenhouse experiment in the phytotron was conducted to evaluate the P supplying capacities of these soils at different temperatures (9, 12 and 18 °C). The crops were grown in succession and the sequence was oat, rye grass (cut twice), oat, rape and oat. Effect of temperature on dry matter (DM) yield and P uptake was more marked up to the fourth crop but the effect varied among crops. The DM yields of oat and rape increased with increasing temperature but the opposite was the case with rye grass. The yield differences among soils at 12 °C were highly significant (p 〈 0.01) in contrast to 9 and 18 °C. The amount of P taken up by plants in these soils was highest at 18. °C. The P supplying capacity was highest in the soils with higher content of organic P. Generally, the soils of very fine and coarse texture classes failed to supply enough P to crops to avoid P deficiency in the successive crops. Soil P test (P-NH4-lactate) values in most of the soils increased with increasing temperatures. The highest temperature effect was seen in the Særheim sand soil. Soil P test extractants P-AL, Bray-1 and Colwell-P were used to determine P in the soil after each harvest and the soil P test values were compared with P uptake by crops. Only the P-AL extractant was significantly correlated to cumulative P removal (CPR) by plants in most of the soils. Regression equation was calculated for each soil. The value of removed P per harvest (RPH) varied from 10.33 to 20.87 mg P kg−1 soil. Phosphorus drawdown slope was determined for each soil and the number of consecutive harvests necessary to reduce the P-AL value to a normal level (110 mg P kg−1 soil) was calculated. The drawdown slope varied widely (1.257–2.801) and this reflected the P buffer capacity and the number of crops required to lower the soil test P value to a normal level. The highest drawdown slope was found in the soils with higher P supplying capacities. The Bray-1 extractant was significantly correlated in the soils with higher buffer capacity but the Colwell-P method did not show significant correlation in any of the soils.
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    Effects of temperature on nutrient release from slow-release fertilizers (1996)
    Springer
    Nutrient cycling in agroecosystems 46 (1996), S. 179-187 
    Details
    ISSN: 1573-0867
    Keywords: electrical conductivity ; leaching ; nitrogen ; pH ; phosphorus ; potassium ; release pattern ; slow-release fertilizers ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We studied the effect of temperature on the release of N, P, and K from slow-release fertilizers (SRF). The study was conducted in micro-lysimeters filled with moist peat medium. Increasing the temperature from 4 to 12°C slightly increased N release from three different slow-release N (SRN) carriers with different particle sizes and coating thicknesses. At 21°C the rate of release was significantly different than the other two temperatures. Urea formaldehyde (UF), sulphur coated urea (SCU) and coated calcium nitrate (CCN), incubated in sphagnum moss peat, released between 3 and 20% of the applied N in six weeks. For eight synthetic and organic NPK carriers, the release pattern was similar to UF and SCU. However, the leaching losses of N from the NPK fertilizers were up to twenty times more than for the SRN products. Except for Osmocote® and Duna, which released 30–40% of the applied N as mineral-N within six weeks, all other slow-release and slowly mineralized NPK carriers acted like readily water-soluble compound NPK. Temperature did not affect the nutrient release from NPK fertilizers.
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    Nitrous oxide (N2O) emissions from soils in warm climates (1995)
    Springer
    Nutrient cycling in agroecosystems 42 (1995), S. 159-163 
    Details
    ISSN: 1573-0867
    Keywords: nitrous oxide ; tropical soils ; agriculture ; N availability ; temperature ; soil water content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract N2O emission rates seem to be higher from soils in warm climates than from soils in temperate climates. Warm and moist conditions promote microbial processes that generate N2O. Clearance of tropical forests enhances N2O formation, but emission measurements from other agricultural operations in the tropics are few. Limiting fertilizer application to recommended rates applied at appropriate times and avoiding fallow land wherever practical serves to limit N2O emissions. More specific advice for agriculture in warm climates requires further studies.
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    Effects of temperature and solar radiation interactions on the survival of quiescent conidia of the entomopathogenic hyphomycetePaecilomyces fumosoroseus (Wize) Brown and Smith (1996)
    Springer
    Mycopathologia 135 (1996), S. 163-170 
    Details
    ISSN: 1573-0832
    Keywords: Entomopathogenic hyphomycete ; Paecilomyces fumosoroseus ; inoculum persistence ; solar radiation ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The detrimental effect of solar radiation on the survival of conidia of the entomopathogenic fungusPaecilomyces fumoroseus was studied by monitoring germinability and ability to form colonies (CFU) of conidia irradiated at two temperatures, 25 and 35 °C, harmless to shaded conidia. There was no apparent effect when spores were exposed to a high level of artificial radiation (0.66 W m−2 UVB). However, at a lower level of irradiance (0.33 W m−2), effects of radiation occurred more quickly at 35 °C than at 25 °C. Under natural solar radiation, the rate of decrease in germinability or viability was doubled at 35 °C as compared to 25 °C, indicating an interaction between temperature and radiation effects under natural conditions. This interaction was not detected in indoor experiments, indicating that the spectral distribution of UV radiation has to be taken in account as well as its irradiance when studying its effects.
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    The dependence of transport properties ofin situ rocks on pore fluid composition and temperature (1996)
    Springer
    Surveys in geophysics 17 (1996), S. 289-306 
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    ISSN: 1573-0956
    Keywords: Effective properties ; pore fluids ; cracked rock ; permeability ; stress ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Fluids saturating cracked rocks within the crust can vary widely in composition and physical properties, which depend greatly on pressure and temperature. External non-hydrostatic stress applied to a cracked medium may result in a significant change of crack volume (and hence, for the undrained regime, pore-fluid pressure) due to the processes of crack closure (opening), and thus lead to a drastic change of the overall physical parameters of a rock. The purpose of the study is to estimate theoretically, using the effective-medium theory, the macroscopic seismic and transport parameters (such as permeability) of cracked rocks (granites) saturated with hydrocarbon gases, oils, brines and water. Variations of crack geometry and fluid parameters in the closed system (at constant fluid mass) under uniaxial compression are considered as well. The results show that composition of a saturating fluid as well as fluid temperature greatly influence the effective permeability and shear velocities of a rock mass, while thermal conductivity is not so sensitive to variations of fluid parameters.
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    Powdered elemental sulphur: oxidation rate, temperature dependence and modelling (1996)
    Springer
    Nutrient cycling in agroecosystems 47 (1996), S. 19-28 
    Details
    ISSN: 1573-0867
    Keywords: elemental sulphur ; model ; oxidation rate ; particle size distribution ; soil ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Finely-powdered elemental sulphur is a useful source of fertilizer S, being readily oxidizable in soil to plant-available sulphate yet possessing some slow release characteristics. Two mesh sizes were evaluated using four soils from northeast Scotland. Particle size analysis found that the 120 and 300 mesh S samples had specific surface areas of 1300 and 1940 cm2 g−1, respectively, with most of the surface area in particles of 10 – 20 µm diameter. The S oxidation rate was similar in all four soils but was greater for the 300 mesh than for the 120 mesh S: mean values of 51% and 18% were oxidized over 7 weeks at 14 °C, respectively. The time course of oxidation followed a sigmoidal pattern with a pronounced lag which was modelled using the logistic equation. Maximum specific oxidation rates were 11–28 µg S cm−2 day−2 for the 300 mesh S at 14 °C. These were significantly slower at 7 °C and the temperature response was calculated as a Q10 of 4.0. A model of seasonal S oxidation was developed using a cosine function for the annual temperature, the Arrhenius equation to relate S oxidation rate to temperature and a generalization of the logistic equation to describe the time course of S oxidation. Simulations showed that the 300 mesh S would be useful for spring S applications in east Scotland and if applied in autumn could supply S during the autumn and again in the spring. The 120 mesh S would be less effective in autumn but more resistant to winter leaching. The 120 mesh S applied to the warmer soils of southwest England would behave the same as 300 mesh S applied in east Scotland.
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    Diapause Induction and Termination in Eggs of the Fruit Tree Red Spider Mite Panonychus Ulmi in Northern Greece (1999)
    Springer
    Experimental and applied acarology 23 (1999), S. 669-679 
    Details
    ISSN: 1572-9702
    Keywords: Panonychus ulmi ; dipause eggs ; overwintering ; dipause termination ; temperature ; photoperiod
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In apple orchards in northern Greece, females of Panonychus ulmi Koch were found to lay diapause eggs from late August to the beginning of October. The course of diapause termination in the field was determined by transferring diapause eggs during winter and early spring from apple orchards with the varieties ‘Starkinson’ and ‘Firiki’ to short days (LD 8:16) (1992–1996), and long days (LD 16:8) (1994–1995), both at 20 °C. Percentages of diapause termination were very low to zero from October to the beginning of January, then progressively increased throughout January and February. Diapause termination in 50% of the eggs occurred in the first half of February in lowland mite populations irrespective of the year and location from which the eggs originated, and about one month earlier in a population originating from an altitude of 300 m. For each sampling date throughout the winter, the mean number of days required for 50% egg hatch at 20 °C (T50%) was similar under either a long (LD 16:8) or a short (LD 8:16) photoperiod. Diapause eggs collected in October 1995 from two orchards and maintained at 0, 5, 10 and 15 °C for various periods were subsequently transferred to 20 °C and LD 8:16, where TP50% was determined. It was shown that temperature, duration of maintenance at the different temperatures and the orchard from which the eggs originated had a significant effect on T50% and therefore on diapause development. Additionally, in our strains diapause intensity was much weaker than in strains from more northern latitudes and was terminated even without any cold exposure. The variation in diapause intensity in different strains of P. ulmi may have an adaptive significance for this widespread species.
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    Effect of temperature on polaronic and bipolaronic structures of the adiabatic Holstein model (1996)
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    Journal of statistical physics 85 (1996), S. 471-488 
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    ISSN: 1572-9613
    Keywords: Electron-phonon interaction ; temperature ; polaron ; Holstein model ; bifurcation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract It is proved that the polaronic and bipolaronic structures found in the adiabatic Holstein model at large electron-phonon coupling by Aubry, Abramovici, and Raimbault survive under connection of the electrons to a low-temperature heat bath, uniformly in the size of the system. Bounds are computed for one-dimensional nearest neighbor chains, and some sample solutions are continued numerically.
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    The growth of Atlantic salmon (Salmo salar L.) myosatellite cells in culture at two different temperatures (1995)
    Springer
    Cellular and molecular life sciences 51 (1995), S. 260-266 
    Details
    ISSN: 1420-9071
    Keywords: Salmon ; satellite cells ; cell culture ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Temperature is known to affect fish growth, and in Atlantic salmon there is an influence on muscle cellularity. Primary muscle cell culture makes it possible to investigate direct effects of temperature on myogenic cells. Salmon myosatellite cells were cultured for the first time in this study. The cells were cultured at either 5°C or 11°C. Increased temperature led to an increase in differentiation rate and especially hypertrophic growth (Q10=4.0). No nuclear proliferation was evident in the satellite cell population isolated at either temperature. This may be due to the presence of different subpopulations of myogenic cells at different developmental ages or the presence of indirect factors in vivo.
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    Age-related changes in gene expression in the rat brain revealed by differential display (1996)
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    Cellular and molecular life sciences 52 (1996), S. 888-891 
    Details
    ISSN: 1420-9071
    Keywords: Ageing ; rat ; brain ; gene expression ; differential display
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have used the polymerase chain reaction (PCR)-based technique of differential display to analyse changes in gene expression during ageing of the rat brain. In this approach we have compared three young adult (6 months) with three old adult (20 months) animals. RNA preparations from the homogenised brains were subjected to reverse transcriptase (RT)-PCR using 36 different combinations of primer pairs. Any PCR product which was consistently found to be more prominent in the three young brains compared to the three old brains, and vice versa, was scored as potentially representing a gene which was differentially expressed during the ageing of this tissue. Out of a possible 2000+PCR products we identified 44 that might represent genes that exhibit differential expression during ageing of the rat brain. An initial screen of these fragments, by Southern-blotting the PCR products and hybridising them with cDNA probes derived from either young or old brain RNA preparations, indicated that 40% of them represented genes that were differentially expressed. This approach is likely to prove invaluable for identifying cohorts of genes that show differential expression during the ageing process.
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    Oxygen patchiness in a lake (1995)
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    Aquatic sciences 57 (1995), S. 325-337 
    Details
    ISSN: 1420-9055
    Keywords: Oxygen ; temperature ; patchiness ; microscale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The presence and extent of small scale vertical structure in dissolved oxygen were investigated in a tropical Australian lake. At a 9 m deep site an extremely high oxygen patch appeared near the bottom boundary. Light levels reaching the bottom boundary during the life of the patch exceeded 10 µEinst m−2 s−1. The patch remained for 1.5 h before gradually reaching ambient oxygen levels. The patch's disappearance coincided with decreasing light levels during the late afternoon. Differences in the extent of oxygen vertical patchiness between deep (16 m) and shallow (9 m) sites were also observed. At the deep site, the distribution of oxygen steps over the water column coincided largely with the distribution of temperature steps. This indicates the dominance of physical processes at that site. At the shallow site, marked vertical patchiness in oxygen was observed with no similar patchiness in temperature. This indicates the prevalence of biological and chemical processes.
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    Stability and expression of amplified EPSPS genes in glyphosate resistant tobacco cells and plantlets (1996)
    Springer
    Plant cell reports 15 (1996), S. 431-436 
    Details
    ISSN: 1432-203X
    Keywords: glyphosate ; gene expression ; gene amplification ; cell culture ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The stability and expression of amplified 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) genes was examined in glyphosate resistant tobacco cells grown in glyphosate-free medium, and in plantlets regenerated from resistant cells. Amplified DNA was maintained in resistant cells grown in the absence of glyphosate for three years. Amplified EPSPS genes were retained in regenerated plantlets at levels comparable to those observed in the resistant cells, and EPSPS mRNA was overexpressed (compared to unselected plantlets). However, glyphosate resistance in cell lines grown in glyphosate-free medium declined 7-fold, and in regenerated plantlets approximately 20-fold, compared to resistant cells maintained under glyphosate selection. In plantlets, reduced resistance correlated with lower levels of EPSPS mRNA. Plantlets regenerated from resistant cells exhibited morphological variation, and had an approximate doubling of their nuclear genome size.
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    Desulforhopalus vacuolatus gen. nov., sp. nov., a new moderately psychrophilic sulfate-reducing bacterium with gas vacuoles isolated from a temperate estuary (1996)
    Springer
    Archives of microbiology 166 (1996), S. 160-168 
    Details
    ISSN: 1432-072X
    Keywords: Key words Anaerobic sulfate reduction ; Incomplete ; propionate oxidation ; Marine environment ; Low ; temperature ; Psychrophilic bacteria ; Growth yields ; Desulforhopalus vacuolatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new type of gas-vacuolated, sulfate-reducing bacterium was isolated at 10° C from reduced mud (E0 〈 0) obtained from a temperate estuary with thiosulfate and lactate as substrates. The strain was moderately psychrophilic with optimum growth at 18–19° C and a maximum growth temperature of 24° C. Propionate, lactate, and alcohols served as electron donors and carbon sources. The organism grew heterotrophically only with hydrogen as electron donor. Propionate and lactate were incompletely oxidized to acetate; traces of lactate were fermented to propionate, CO2, and possibly acetate in the presence of sulfate. Pyruvate was utilized both with and without an electron acceptor present. The strain did not contain desulfoviridin. The G+C content was 48.4 mol%. The differences in the 16S rRNA sequence of the isolate compared with that of its closest phylogenetic neighbors, bacteria of the genus Desulfobulbus, support the assignment of the isolate to a new genus. The isolate is described as the type strain of the new species and genus, Desulforhopalus vacuolatus.
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    Carbon dioxide evolution of an upland rice and barley, double cropping field in central Japan (1996)
    Springer
    Ecological research 11 (1996), S. 217-227 
    Details
    ISSN: 1440-1703
    Keywords: double cropping field ; soil respiration ; temperature ; water content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Carbon dioxide evolution rates from a double cropping, upland rice and barley field were determined in central Japan from June 1992 to May 1994, and regression models were developed to predict soil respiration rate. Diurnal patterns of hourly soil respiration rates (SRh) showed a similar trend with those of soil surface temperatures. Daily soil respiration rate (SRd) obtained by integrating SRh varied from 0.3 to 15.6 g CO2 m−2 for the 2 years. In the summer cropping period, SRd was positively correlated with daily mean soil surface temperature and negatively correlated with volumetric water content in soil. Moreover, this relationship was able to be expressed as a multiple-factor model with an Adj-R2 of 0.925. On the other hand, in the winter cropping period, SRd was able to be represented by a single factor model using soil surface temperature with an Adj-R2 of 0.854. Based on these relationships, seasonal changes in soil respiration rate were estimated. Total soil respiration rates in 1992 and 1993 estimated for the summer cropping period were 1260 g CO2 m−2 and 1094 g CO2 m−2, and for the winter cropping period 624 g CO2 m−2 and 676 g CO2 m−2, respectively. It was considered that the lower values during the summer cropping period in 1993 depended on lower soil surface temperature and higher soil water content.
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    Comparative studies on some analytical methods (1997)
    Springer
    Journal of thermal analysis and calorimetry 49 (1997), S. 857-862 
    Details
    ISSN: 1572-8943
    Keywords: analytical methods ; kinetics ; powder milk ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract This work present comparative results on powder milk storage quality, obtained from analytical methods. Protein content was determined conventional (Kjeldahl) and colorimetric with biuret reagent at 540 nm and integral quality by thermogravimetric and biological methods. A method was developed for the protein separation of powder milk. Powder milk was submitted to degradation processes at 45, 60 and 80°C for 20 days. The results indicated that protein content values were inconsistent if determinations by Kjeldahl and colorimetric methods and biological tests were compared. There is evidence of thermal decomposition of powder milk as detected by biological and thermogravimetric methods.
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    Thermodynamics of Polyvinylacetate from 0 to 350 K (1998)
    Springer
    Journal of thermal analysis and calorimetry 54 (1998), S. 731-740 
    Details
    ISSN: 1572-8943
    Keywords: enthalpy ; entropy ; Gibbs energy ; heat capacity ; limiting polymerization temperature ; polyvinylacetate ; temperature ; thermodynamics of polymerization ; vinyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The temperature dependence of the heat capacity of vinyl acetate in the range 13 to 330 K and of polyvinylacetate between 4.9 and 330 K was determined by adiabatic vacuum calorimetry with an error of about 0.2%. Temperatures and enthalpies of physical transitions were measured. From the data obtained, the thermodynamic characteristics of melting of vinyl acetate and parameters of glass transition and glassy state of the monomer and polymer were calculated. The thermodynamic functions Ho(T)-Ho(0), So(T), Go(T)-Ho(0) were estimated for both materials from 0 to 350 K. The results of calculation and the literature value of enthalpy of bulk polymerization of vinyl acetate at T=350 K were used for the estimation of the thermodynamic parameters of its polymerization process ΔHpol o, ΔSpol o, ΔGpol oin the interval 0 to 350 K. A ceiling limiting temperature of polymerization Tceil owas evaluated.
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    Characterization of a twin-torch transferred dc arc (1995)
    Springer
    Plasma chemistry and plasma processing 15 (1995), S. 309-331 
    Details
    ISSN: 1572-8986
    Keywords: Transferred arc ; voltage-current characteristic ; inhomogeneous plasma ; optical emission spectroscopy ; electron density ; temperature ; spatial distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract The results of a twin-torch transferred de arc .study are presented. The arc system consists of two torches of opposite polarity, and a coupling zone of plasma jets located between them. The torch configuration increases the system reliability and efficiency during material plasma processing. The results of the study present data for the voltage-current characteristics, general behavior of the twin-torch arc, and spatial distribution of the plasma parameters. The plasma parameters have been measured using optical emission spectroscopy for a 200 A (20 k W) do arc, at atmospheric pressure, with argon and nitrogen introduced as plasma forming gases into the anode and the cathode units, respectively. The measurement technique used has allowed the determination of local electron density and temperature values in an inhomogeneous plasma volume having no axial sysmmetry. The data obtained illustrate the novel features of the twin-torch transfrred do arc for its applications in plasma processing.
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    Hydrogen/argon plasma jet with methane addition (1995)
    Springer
    Plasma chemistry and plasma processing 15 (1995), S. 333-351 
    Details
    ISSN: 1572-8986
    Keywords: Hydrogen/argon plasma ; methane ; do arc jet ; atmospheric pressure ; optical emission spectroscopy ; temperature ; density ; spatial distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract Spatial distributions of plasma parameters are presented for a H2/Ar plasma jet with addition of methane. The plasma has been generated at atmospheric pressure by a 200 A (20 kW) nontransferred do arc. Optical emission spectroscopy has been used for the measurements assuming the plasma jet to be optically thin and to have an axial symmetry. Local spectral ernissivity values have been evaluated using a routine Abel inversion procedure. Half- width and emissivity of Hβ spectral line have been measured to determine the electron density and temperature of the plasma. The densities of excited C, CH radicals have been evaluated from the absolute emissivities of relevant molecular emission bands measured in limited spectral intervals in the visible spectrum. The emissivity ratios have been used to fund rotational and vibrational temperatures. The results supply information on methane decomposition and the behavior of molecular radicals in close-to-thermal plasma jets.
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    Measurement by LASER-Generated Ultrasound of Four Stiffness Coefficients of an Anisotropic Material at Elevated Temperatures (1997)
    Springer
    Journal of nondestructive evaluation 16 (1997), S. 91-100 
    Details
    ISSN: 1573-4862
    Keywords: LASER ultrasound ; anisotropic material ; group velocity ; stiffness coefficients ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Mathematics
    Notes: Abstract Ultrasonic waves are generated through a composite material by means of a noncontact technique. It uses a Nd:Yag LASER for the generation and an interferometric probe for the detection of acoustic waveforms. From a suitable set of experimental data, an inversion scheme is used for the recovering of four stiffness coefficients. They characterize the elasticity in a principal plane of symmetry of the material which exhibits an orthorhombic symmetry. The measurements are performed at various temperatures, elevated by steps up to 300°C for two specimen. The sensitivity of the method appears convenient to measure the temperature induced stiffness changes. The anisotropic degradation of the material properties are then pointed out.
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    Expression of mRNA for vasoactive intestinal peptide in normal human colon and during inflammation (1995)
    Springer
    Molecular and cellular biochemistry 142 (1995), S. 1-7 
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    ISSN: 1573-4919
    Keywords: vasoactive intestinal peptide ; ulcerative colitis ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The availability of colon provides a ready source of human neurons. Among the products of nerve cell bodies, vasoactive intestinal peptide is a neuropeptide that serves as a marker of non-adrenergic, non-cholinergic inhibitory nerves in colon. These nerves have been proposed to be involved in regulation of immune function, secretion, and smooth muscle function. In previous work, we identified decreased tissue levels of vasoactive intestinal peptide in a disorder of chronic colonic mucosal inflammation, ulcerative colitis. We hypothesized that diminished gene expression of vasoactive intestinal peptide could result in decreased tissue levels of this neuropeptide. Sigmoid colon was obtained at surgery from controls (n=6) and patients with ulcerative colitis (n=6). Vasoactive intestinal peptide mRNA was quantified by Northern blot hybridization and tissue levels of vasoactive intestinal peptide were determined by radioimmunoassay. Tissue vasoactive intestinal peptide was decreased only in the mucosalsubmucosal layer of ulcerative colitis (p=.02). There was a single 1.7 kbase vasoactive intestinal peptide transcript identified in both control colon and ulcerative colitis. Normalized vasoactive intestinal peptide mRNA levels were increased by 260% in ulcerative colitis compared to controls (p〈.01). These observations suggest that decreased vasoactive intestinal peptide gene expression or abnormal post-transcriptional processing are not primary defects in this disorder of chronic inflammation. The findings support the alternative hypothesis that axonal degeneration in ulcerative colitis could result in increased expression of neuronal vasoactive intestinal peptide mRNA.
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    URL: http://dx.doi.org/10.1007/BF00928907
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    Expression of hepatic calcium-binding protein regucalcin mRNA is elevated by refeeding of fasted rats: Involvement of glucose, insulin and calcium as stimulating factors (1995)
    Springer
    Molecular and cellular biochemistry 142 (1995), S. 35-41 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; insulin ; calcium ; gene expression ; rat liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effect of refeeding on the expression of Ca2+-binding protein regucalcin mRNA in the liver of fasted rats was investigated. When rats were fasted overnight, the hepatic regucalcin mRNA level was reduced about 70% of that in feeding rats. Refeeding produced a remarkable elevation of hepatic regucalcin mRNA level (about 150–170% of fasted rats). Liver regucalcin concentration was appreciably increased by refeeding, although it was not altered by fasting. The oral administration of glucose (2 g/kg body weight) to fasted rats caused a significant increase in hepatic regucalcin mRNA level. Moreover, hepatic regucalcin mRNA level was clearly elevated by a single subcutaneous administration of insulin (10 and 100 U/kg) to fasted rats. The hormonal effect was not further enhanced by the simultaneous administration of calcium chloride (250 mg Ca/kg) to fasted rats, although calcium administration stimulated regucalcin mRNA expression in the liver. The present study suggests that the expression of hepatic regucalcin mRNA stimulated by refeeding is significantly involved in the action of insulin and/or calcium as stimulating factors.
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    URL: http://dx.doi.org/10.1007/BF00928911
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    Specific species and tissue differences for the gene expression of calcium-binding protein regucalcin (1995)
    Springer
    Molecular and cellular biochemistry 143 (1995), S. 67-71 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene expression ; gene distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The existence and expression of gene encoding the Ca2+-binding protein regucalcin in various species and tissues were investigated with Southern and Northern hybridization analyses using regucalcin cDNA (0.9 kb of open reading frame). Genomic Southern hybridization analysis demonstrated that regucalcin gene was widely conserved among higher animals including human, monkey, rat, mouse, dog, bovine, rabbit and chicken. The gene was not found in yeast. The Northern blot analysis of poly (A)+RNAs extracted from the liver of various species showed that regucalcin mRNA was predominantly expressed in rat and mouse, although the expression was also seen in human, bovine and chicken. Furthermore, the enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG indicated that hepatic regucalcin concentration was most pronounced in rat as compared with that of guinea pig, mouse and chicken. These observations show that the gene expression of regucalcin and its protein synthesis is unique in the liver of rats, suggesting the existence of a specific mechanism in demonstrating regucalcin synthesis from gene.
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    URL: http://dx.doi.org/10.1007/BF00925928
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    17β-Estradiol stimulates the expression of hepatic calcium-binding protein regucalcin mRNA in rats (1995)
    Springer
    Molecular and cellular biochemistry 143 (1995), S. 137-141 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; estrogen ; gene expression ; rat liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effect of nuclear receptor-related hormones on the expression of hepatic calcium-binding protein regucalcin mRNA in rats was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin cDNA (0.9 kb of open-reading frame). A single subcutaneons administration of 17β-estradiol (0.5, 1.0 and 2.0 mg/kg body weight) in rats induced a remarkable increase of regucalcin mRNA in liver; the level was about 200% of control at 24 h after the administration of 2.0 mg/kg. The increase showed about 350% even at 6 h after the administration. Meanwhile, hepatic regucalcin mRNA level was not appreciably altered by a single subcutaneous administration of thyroxine (T4) (20, 40 and 80 mg/kg) or hydrocortisone (10 and 30 mg/kg) in rats. The present study demonstrates that the expression of hepatic regucalcin mRNA is stimulated by estrogen action in the liver nuclei of rats.
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    URL: http://dx.doi.org/10.1007/BF01816947
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    Thyroid hormone inhibits fatty acid synthase gene transcription in chicken liver (1995)
    Springer
    Molecular and cellular biochemistry 144 (1995), S. 105-108 
    Details
    ISSN: 1573-4919
    Keywords: fatty acid synthase ; gene expression ; and thyroid hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effect of triiodothyronine (T3) on regulation of fatty acid synthase in chicken liver was investigated. In hypothyroid animals, enzyme activity was about one half of that in euthyroid animals. T3 treatment increased the enzyme activity in hypothyroid animals. There is little difference in both the mRNA concentration and the transcription rate between euthyroid and hypothyroid animals. T3 treatment markedly decreased both the mRNA concentration and the transcription rate in euthyroid and hypothyroid animals. These results suggested that T3 maintained the normal level of enzyme expression primarily by stimulating the post-transcriptional step, while the transcription of the gene was inhibited by hyperthyroidism.
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    URL: http://dx.doi.org/10.1007/BF00944388
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    Expression of calcium-binding protein regucalcin mRNA in hepatoma cells (1996)
    Springer
    Molecular and cellular biochemistry 155 (1996), S. 85-90 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene expression ; rat hepatoma ; Morris hepatoma cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Whether the gene expression of hepatic Ca2+-binding protein regucalcin is altered in hepatomas was investigated. The change in regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin complementary DNA (0.9 kb). Rat hepatoma was induced by continuous feeding of basal diet containing 0.06% 3′-methyl-4-dimethylaminoazobenzene (3′-Me-DAB). After 35 weeks feeding, rats were sacrificed, and the non-tumorous and tumorous tissues of the livers were removed. In individual rats, the regucalcin mRNA levels in the tumorous tissues were generally decreased in comparison with that of the non-tumorous tissues of the chemical-fed rats, although the chemical administration might decrease the mRNA expression in normal rat liver, suggesting that the chemical administration causes a suppresive effect on the mRNA expression. When the genomic DNA extracted from the liver tumorous tissues was digested with restriction enzymes (EcoRI, BamHI and HindIII) and analyzed by Southern blotting, no rear-ranged band was found in the regucalcin gene from the hepatoma. Interestingly, in the transplantable Morris hepatoma cells, the regucalcin mRNA was markedly expressed, while the albumin mRNA was expressed only slightly. The present study demonstrates that regucalcin mRNA is clearly expressed in the transformed cells (Morris hepatoma cells).
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    URL: http://dx.doi.org/10.1007/BF00714337
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    Steroid hormonal regulation of calcium-binding protein regucalcin mRNA expression in the kidney cortex of rats (1996)
    Springer
    Molecular and cellular biochemistry 155 (1996), S. 105-111 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; aldosterone ; estrogen ; dexamethasone ; gene expression ; rat kidney cortex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effect of various steroid hormones on the expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using rat liver regucalcin complementary DNA (0.9 kb of open-reading frame). Regucalcin mRNA was expressed in the kidney cortex but not the medulla. Rats received a single subcutaneous administration of steroid; the animals were sacrificed 60 min after the treatment of aldosterone (2.5, 5.0 and 10 μg/100 g body weight) or 6 h after the treatment of estrogen (17β-estradiol; 0.05, 0.1 and 0.2 mg/100 g), hydrocortisone (0.5, 1.0 and 3.0 mg/100 g) and dexamethasone (50, 100 and 150 μg/100 g). Regucalcin mRNA levels in the kidney cortex were clearly diminished by the administration of aldosterone or estrogen, while hydrocortisone administration had no effect. The administration of dexamethasone (100 μg/100 g) caused a remarkable increase of regucalcin mRNA levels in the kidney cortex. The dexamethasone-induced increase in regucalcin mRNA levels was completely blocked by the simultaneous administration of cycloheximide (150 μg/100 g), although the drug administration had no effect on the mRNA levels in control rats. Meanwhile, the dexamethasone administration did not cause an appreciable alteration of calcium content in the kidney cortex. The present study demonstrates that, of the various steroid hormones used, dexamethasone uniquely has a stimulatory effect on regucalcin mRNA expression in the kidney cortex of rats. The steroid effect may be mediated through a newly synthesized protein.
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    URL: http://dx.doi.org/10.1007/BF00229307
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    Estrogen effects in the heart (1996)
    Springer
    Molecular and cellular biochemistry 160-161 (1996), S. 307-313 
    Details
    ISSN: 1573-4919
    Keywords: myocardium ; hypertension ; gene expression ; estrogens ; cardiac hypertrophy ; signal transduction ; genetic program
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Gender specific differences in cardiovascular disease are largely mediated by sex hormones. The use of estrogens significantly reduces the overall incidence of heart disease in postmenopausal women. Beneficial effects of estrogens on plasma lipoprotein levels are clearly established. However, these do not explain the magnitude of risk reduction seen in clinical studies. Thus, additional and currently unknown functions of estrogens must be operative. Elucidation of the exact estrogen action in the heart will have important implications in the treatment of cardiovascular disease. It will probably enhance the therapeutic repertoire in treating heart disease, the most common cause of death in industrialized countries. We will review the current understanding of the function of estrogens in the heart and discuss potential strategies on how to apply these data to clinical practice.
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    URL: http://dx.doi.org/10.1007/BF00240064
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    Calcium-binding protein regucalcin mRNA expression in the kidney cortex is suppressed by saline ingestion in rats (1996)
    Springer
    Molecular and cellular biochemistry 162 (1996), S. 139-144 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene expression ; saline ingestion ; hypertensive rats ; kidney cortex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The effect of adrenalectomy (ADX) or saline ingestion, which is a hypertensive factor, on the expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using rat liver regucalcin complementary DNA (0.9 kb of open-reading frame). Regucalcin mRNA was expressed in the kidney cortex but not the medulla. Rats were adrenalectomized, and 48 h later they were sacrificed. ADX caused a reduction of regucalcin mRNA levels in the kidney cortex, suggesting that adrenal glands participate in the regulation of the mRNA expression. This reduction was not restored by the subcutaneous administration of dexamethasone with an effective dose (1 mg/kg body weight), which can stimulate kidney regucalcin mRNA expression. Regucalcin mRNA levels in the kidney cortex of rats were markedly suppressed by the ingestion of saline for 7 days. The ADX-induced decrease of renal cortex regucalcin mRNA levels was not appreciably restored by saline ingestion. Moreover, regucalcin mRNA levels in the kidney cortex of spontaneous hypertensive rats (SHR) were clearly decreased as compared with that of control (Wistar-Kyoto) rats. Meanwhile, calcium content in the kidney cortex was not significantly decreased by ADX or saline ingestion. The present study suggests that the expression of regucalcin mRNA in the kidney cortex of rats is suppressed by saline administration.
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    URL: http://dx.doi.org/10.1007/BF00227541
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    Calreticulin inhibits glucocorticoid– but not cAMP–sensitive expression of tyrosine aminotransferase gene in cultured McA–RH7777 hepatocytes (1997)
    Springer
    Molecular and cellular biochemistry 171 (1997), S. 37-43 
    Details
    ISSN: 1573-4919
    Keywords: calreticulin ; gene expression ; steroid receptor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Calreticulin is a ubiquitously expressed Ca2+ binding protein of the endoplasmic reticulum which inhibits DNA binding and transcriptional activation by steroid hormone receptors. In this study the effects of calreticulin on tyrosine aminotransferase (TAT) gene expression in cultured McA–RH7777 hepatocytes was investigated. McA–RH7777 cells were stably transfected with calreticulin expression vector to generate cells overexpressing the protein. The transcriptional activity of the TAT gene, which is glucocorticoid–sensitive and cAMP–dependent, was investigated in the mock transfected McA–RH7777 and in cells overexpressing calreticulin (designated McA–11 and McA–17). In the presence of dexamethasone or the cAMP analog (CTP–cAMP) expression of the TAT gene was induced in mock transfected McA–RH7777 cells by approximately 4.5 and 5 fold, respectively. In McA–11 and McA–17 cells, overexpressing calreticulin, glucocorticoi ever, the CTP–cAMP–dependent expression of the TAT gene was not affected. The ability of calreticulin to inhibit glucocorticoid–sensitive TAT gene expression but not the cAMP–dependent expression of the gene suggests that the protein affects specifically the action of transcription pathways involving steroid receptors or transcription factors containing KxFF(K/R)R–like motifs. Calreticulin may play an important role in the regulation of glucocorticoid–sensitive pathway of expression of the hepatocytes specific genes during development.
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    URL: http://dx.doi.org/10.1023/A:1006865108833
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    Thiol modulation of TNFα and IL-1 induced MnSOD gene expression and activation of NF-κB (1995)
    Springer
    Molecular and cellular biochemistry 148 (1995), S. 45-57 
    Details
    ISSN: 1573-4919
    Keywords: manganese ; superoxide dismutase ; gene expression ; hyperoxide lung injury ; nuclear factor kappa B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract TNFα and IL-1 each can activate NF-κB and induce gene expression of manganese superoxide dismutase (MnSOD), a mitochondrial matrix enzyme which can provide critical protection against hyperoxic lung injury. The regulation of MnSOD gene expression is not well understood. Since redox status can modulate NF-κB and potential κB site(s) exist in the MnSOD promoter, the effect of thiols (including NAC, DTT and 2-ME) on TNFα and IL-1 induced activation of NF-κB and MnSOD gene expression was investigated. Activation of NF-kB and increased MnSOD expression were potentiated by thiol reducing agents. In contrast, thiol oxidizing or alkylating agents inhibited both NF-κB activation and elevated MnSOD expression in response to TNFα or IL-1. Since protease inhibitors TPCK and TLCK can inhibit NF-κB activation, we also investigated the effect of these compounds on MnSOD expression and NF-κB activation. TPCK and TLCK each inhibited MnSOD gene expression and NF-κB activation. Since the MnSOD promoter also contains anAP-1 binding site, the effect of thiols and thiol modifying agents on AP-1 activation was investigated. Thiols had no consistent effect onAP-1 activation. Likewise, some of the thiol modifying compounds inhibited AP-1 activation by TNFα or IL-1, whereas others did not. Since diverse agents had similar effects on activation of NF-κB and MnSOD gene expression, we have demonstrated that activation of NF-κB and MnSOD gene expression are closely associated and that reduced sulfhydryl groups are required for cytokine mediation of both processes.
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    URL: http://dx.doi.org/10.1007/BF00929502
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    Stimulatory effect of calcium administration on regucalcin mRNA expression is attenuated in the kidney cortex of rats ingested with saline (1998)
    Springer
    Molecular and cellular biochemistry 178 (1998), S. 275-281 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene expression ; calmodulin ; spontaneous hypertensive rats ; rat kidney cortex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats ingested with saline was investigated. The alteration in regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin complementary DNA (0.9 kb of open reading frame). Rats were freely given saline as drinking water for 7 days. Regucalcin mRNA levels in the kidney cortex were suppressed by saline ingestion. When calcium chloride (10 mg Ca/100 g body weight) was intraperitoneally administered to rats ingested with saline for 7 days, the effect of calcium administration to increase regucalcin mRNA levels was weakened by saline ingestion. Such effect was also seen by the administration of 2.5 and 5 mg Ca/100 g. Regucalcin mRNA levels in the kidney cortex of spontaneous hypertensive rats (SHR) were not appreciably increased by the administration of calcium (10 mg/100 g). Meanwhile, calcium content in the kidney cortex was significantly elevated by the administration of calcium (10 mg/100 g) to normal rats. This increase was weakened in saline-ingested rats. Moreover, Ca2+/calmodulin-dependent protein kinase activity in the cytosol of kidney cortex was significantly decreased by saline ingestion. These results suggest the possibility that saline ingestion-induced suppression of regucalcin mRNA expression in the kidney cortex is partly involved in the attenuation of Ca2+ signalling.
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    URL: http://dx.doi.org/10.1023/A:1006898910955
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    Increased peroxisomal fatty acid β-oxidation and enhanced expression of peroxisome proliferator-activated receptor-α in diabetic rat liver (1999)
    Springer
    Molecular and cellular biochemistry 194 (1999), S. 227-234 
    Details
    ISSN: 1573-4919
    Keywords: microbodies ; diabetes mellitus ; steroid hormone receptor ; β-oxidation ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract To determine whether the increased fatty acid β-oxidation in the peroxisomes of diabetic rat liver is mediated by a common peroxisome proliferation mechanism, we measured the activation of long-chain (LC) and very long chain (VLC) fatty acids catalyzed by palmitoyl CoA ligase (PAL) and lignoceryl CoA ligase and oxidation of LC (palmitic acid) and VLC (lignoceric acid) fatty acids by isotopic methods. Immunoblot analysis of acyl-CoA oxidase (ACO), and Northern blot analysis of peroxisome proliferator-activated receptor (PPAR-α), ACO, and PAL were also performed. The PAL activity increased in peroxisomes and mitochondria from the liver of diabetic rats by 2.6-fold and 2.1-fold, respectively. The lignoceroyl-CoA ligase activity increased by 2.6-fold in diabetic peroxisomes. Palmitic acid oxidation increased in the diabetic peroxisomes and mitochondria by 2.5-fold and 2.7-fold, respectively, while lignoceric acid oxidation increased by 2.0-fold in the peroxisomes. Immunoreactive ACO protein increased by 2-fold in the diabetic group. The mRNA levels for PPAR-α, ACO and PAL increased 2.9-, 2.8- and 1.6-fold, respectively, in the diabetic group. These results suggest that the increased supply of fatty acids to liver in diabetic state stimulates the expression of PPAR-α and its target genes responsible for the metabolism of fatty acids.
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    URL: http://dx.doi.org/10.1023/A:1006930513476
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    Towards a Predictive Design Methodology Based on the Physical Modelling of the Fracture of Fiber Composites (1998)
    Springer
    Applied composite materials 5 (1998), S. 69-94 
    Details
    ISSN: 1573-4897
    Keywords: modelling ; notch strength ; damage ; fatigue ; environment ; temperature ; prediction ; design
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A predictive design methodology based on modelling the fracture stress (notched tensile strength) and post-fatigue residual strength of laminated fiber composites is presented. The approach is based explicitly on the development of models of the physical processes by which damage accumulates at a notch-tip and the application of these models to cross-ply laminates for a variety of material systems, including thermosetting and thermoplastic matrices containing carbon, glass and Kevlar fiber reinforcements. The effects of temperature and humidity on composite fracture can also be examined in the context of this modelling strategy. A pre-requisite of the model is that it has to be calibrated for each material system by performing tensile tests on notched and unnotched cross-ply laminate. From this initial calibration, which takes relatively little time, it is possible to apply the model to a prediction of the dependence of fracture stress on notch size; to an understanding of the effects of laminate stacking sequence (within the same cross-ply family) on fracture stress; and to provide insight into the effects of thermal or load cycling history on fatigue damage-growth and residual or fatigue strength. The advantages and deficiencies of this modelling strategy are assessed, as well as the applicability of such a physical modelling approach to the predictive design and failure of composite materials in general.
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    URL: http://dx.doi.org/10.1023/A:1008811721083
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    Regulation of the apolipoprotein E by dietary lipids occurs by transcriptional and post-transcriptional mechanisms (1996)
    Springer
    Molecular and cellular biochemistry 155 (1996), S. 153-162 
    Details
    ISSN: 1573-4919
    Keywords: apolipoprotein B and E ; lipid ; gene expression ; rat ; mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The aim of the present investigation was to study the regulation of apolipoprotein E by two dietary nutrients, saturated fat and cholesterol, known to raise plasma cholesterol levels. ApoE is a protein component of several classes of lipoproteins including VLDL and HDL, and dietary lipids may regulate VLDL and apoE-containing HDL particles through their effects on apoE gene. Male rats and mice were fed the following 4 diets: control diet (C); high cholesterol diet with 0.5% cholesterol (HC); high fat diet with 20% hydrogenated coconut oil (HF); and high fat plus high cholesterol diet with 0.5% cholesterol and 20% fat (HF/C). Plasma cholesterol levels remained unchanged on HC diet, but in mice VLDL-cholesterol increased by 31%. HF diet increased VLDL and LDL by 15–17% in rats, and 21% in mice. A combination of fat and cholesterol diet showed pronounced effects on plasma lipoprotein concentrations, raising apoB-containing particles by 21% and 44% in mice and rats, respectively. Plasma apoE levels increased significantly on all diets. The mechanism of regulation of increased plasma apoB and apoE levels was examined. Quantification of hepatic apoB mRNA showed a lack of correlation between plasma apoB and hepatic apoB mRNA levels, suggesting that posttranscriptional regulation increased plasma apoB-containing lipoproteins in animals fed saturated fat diets. Hepatic apoE mRNA levels increased significantly in animals fed cholesterol-rich diets. However, despite increased plasma apoE levels on diet containing only saturated fat, hepatic apoE mRNA did not change. Synthesis of apoE on the liver polysomes increased selectively on cholesterol-rich diets. These results suggest that cholesterol-rich diets altered apoE, in part, by transcriptional mechanism, and saturated fat-rich diets increased plasma apoE levels by posttranscriptional mechanism, possibly decreased receptor-mediated uptake of apoE-containing particles. The regulation of LDL receptor was also studied since plasma apoB and E levels may be altered by LDL receptor-mediated uptake by the hepatocytes. As expected, high cholesterol diet decreased LDL receptor mRNA by 30–40%. However, the LDL receptor protein on liver membranes did not change on any of the test diets in both animal species. Hepatic cholesterol content increased several fold selectively on high cholesterol diets. These findings suggest that: 1) both transcriptional and posttranscriptional mechanisms are important in regulating plasma apoB and E containing lipoproteins; 2) dietary cholesterol regulates apoE gene by a transcriptional mechanism anddietary saturated fat by posttranscriptional mechanism; and 3) changes in the hepatic apoE and LDL receptor mRNA are associated with the changes in intracellular cholesterol concentrations.
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    Expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats: The stimulation by calcium administration (1995)
    Springer
    Molecular and cellular biochemistry 146 (1995), S. 71-77 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene expression ; rat kidney cortex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using liver regucalcin complementary DNA (0.9 kb of open-reading frame). Regucalcin mRNA was expressed in the kidney cortex, and this expression was clearly increased by a single intraperitoneal administration of calcium chloride solution (5–15 mg Ca/100 g body weight) in rats; this increase was remarkable at 60–120 min after the administration. Thyroparathyroidectomy (TPTX) caused a slight decrease of regucalcin mRNA levels in the kidney cortex. However, the administration of calcium (10 mg/100 g) in TPTX rats produced a clear increase of regucalcin mRNA levels in the kidney cortex. The subcutaneous administration of calcitonin (10–100 MRC mU/100 g) or parathyroid hormone [1–34] (1–10 U/100 g) in TPTX rats which received calcium (10 mg/100 g) administration did not cause an appreciable alteration of regucalcin mRNA levels in the kidney cortex, suggesting that the mRNA expression is not stimulated by calcium-regulating hormones. The administration of trifluoperazine (TFP; 5 mg/100 g), an inhibitor of Ca2+/calmodulin action, completely blocked the expression of regucalcin mRNA stimulated by calcium administration. Now, calcium content in the kidney cortex was significantly elevated by a single intraperitpneal administration of calcium (10 mg/100 g) in rats. The present study clearly demonstrates that the expression of regucalcin mRNA in the kidney cortex is stimulated by calcium administration in rats. This expression may be mediated through Ca2+/calmodulin action in the kidney cortex.
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    URL: http://dx.doi.org/10.1007/BF00926884
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    Molecular cloning, sequencing and expression analysis of a fatty acid transport gene in rat heart induced by ischemic preconditiong and oxidative stress (1996)
    Springer
    Molecular and cellular biochemistry 160-161 (1996), S. 241-247 
    Details
    ISSN: 1573-4919
    Keywords: fatty acid transport protein ; gene expression ; subtractive hybridization ; oxidative stress ; ischemia/reperfusion ; ischemic preconditioning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract In this study, ischemia and oxidative stress-inducible gene expression in heart was examined by subtractive hybridization technique. Total RNA was isolated from ventricular muscle fragments of normal and oxidative stress-induced hearts. Poly (A)+ RNA was purified followed by the construction of a plasmid cDNA library. This was followed by the subtractive screening of oxidative stress-induced cDNA library. The positive colonies were amplified and the plasmid isolated. An aliquot was subjected to restriction cutting with Bam H1 and EcoRl; the fragments corresponding to cDNA insert were separated by electrophoresis, radiolabeled by random-primed DNA synthesis, and used as probes in standard Northern blotting experiments. An aliquot containing the plasmid from the confirmed positives was then subjected to bidirectional partial DNA sequencing (using M13 and T7/T3α primers) by the chain-extension/chain termination method. These sequences were subjected to a computerized search for homologies against all sequences in the updated worldwide Gen Bank and EMBL sequence databases followed by restriction mapping and reading frame identification. Out of 24 putative positive colonies screened, one clone was matched with 〉 97% homology with FAT gene that has been implicated in binding or transport of long chain fatty acids. cDNA probe synthesized from this clone identified two major transcripts of 4.8 and 2.9 kb. Additional experiments were then performed where isolated perfused rat hearts were subjected to the following treatments: (1) 5 min ischemia; (2) 10 min ischemia; (3) 20 min ischemia; (4) 5 min ischemia followed by 10 min reperfusion (ischemic preconditioning); and (5) 5 min ischemia followed by 10 min reperfusion, repeated four times (4 × preconditioning). RNAs were extracted from these hearts and hybridized with the FAT cDNA probe. The results indicated that FAT gene was induced by oxidative stress, ischemic preconditioning, but not by ischemia. (Mol Cell Biochem 160/161: 241–247, 1996)
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    Hepatic calcium-binding protein regucalcin concentration is decreased by streptozotocin-diabetic state and ethanol ingestion in rats (1997)
    Springer
    Molecular and cellular biochemistry 168 (1997), S. 67-72 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; gene expression ; diabetic state ; ethanol ; liver injury
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The alteration in calcium-binding protein regucalcin in the liver and serum of rats with streptozotocin (STZ)-diabetic state or ethanol ingestion was investigated. STZ (6.0 mg/100 g body weight) was subcutaneously administered in rats, and 1 or 3 weeks later they were sacrificed by bleeding. Liver regucalcin mRNA levels were not clearly altered by the diabetic state, as evidenced by Northern blotting using regucalcin cDNA (0.9 kb of open reading frame). Based on enzyme-linked immunoadsorbent assay (ELISA) with rabbit-anti-regucalcin IgG, hepatic regucalcin concentration was decreased about 50% of control levels by STZ treatment. However, serum regucalcin concentration was not significantly altered by STZ treatment. Meanwhile, when rats ingested ethanol (10 and 30%) in the drinking water for 2 weeks, liver regucalcin mRNA levels were clearly increased, although hepatic regucalcin concentration was significantly decreased. Serum regucalcin concentration was not appreciably altered. Serum transaminases (GOT and GPT) activities were significantly increased at 1 or 3 weeks after STZ administration in rats, while their activities were not altered by ethanol ingestion. The present study demonstrates that hepatic regucalcin concentration is decreased independent of mRNA expression in the STZ-diabetes and during ethanol ingestion in rats.
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    Effect of angiotensin II on myocardial collagen gene expression (1996)
    Springer
    Molecular and cellular biochemistry 163-164 (1996), S. 231-237 
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    ISSN: 1573-4919
    Keywords: extracellular matrix ; angiotensin II ; fibrillar collagen ; cardiac fibrosis ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Recent studies suggest that angiotensin II (angiotensin) may be involved in the regulation of metabolism of the cardiac extracellular matrix (ECM). Two major components of ECM are collagen types I and III which play an important role in maintaining the structure and function of the heart. Although the cellular metabolism of collagen is very complex (especially at the posttranslational level), we chose to address events that occur relatively early in the synthesis of cardiac collagen molecules. To gain an understanding of the role of angiotensin in the regulation of cardiac collagen gene expression, we studied the effect of three different doses of angiotensin (12, 24, and 48 μg/kg/h) on adult heart and cultured neonatal cardiac fibroblasts. The steady-state mRNA abundance of collagen types I and III was monitored using Northern blot analysis in both left and right ventricular samples at day 3 of angiotensin infusion and in cultured cardiac fibroblasts stimulated with angiotensin. In all mRNA abundance studies, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) signal was used to normalize the data for possible differences in loading and/or transfer of total RNA. Both collagen types I/GAPDH and III/GAPDH mRNA signal ratios were increased significantly in left ventricle in all dose regimens used for angiotensin infusion. Only the collagen type I/GAPDH mRNA signal ratio was increased in right ventricle with angiotensin infusion. Angiotensin (10−7-10−5 M) had no effect on the steady-state mRNA abundance of collagen genes in cultured neonatal cardiac fibroblasts after 24 h treatment in serum-free conditions. Our results confirm that infusion of angiotensin may upregulate steady-state collagen gene mRNA abundance in the heart. Angiotensin had no observable effect on collagen mRNA abundance in neonatal fibroblast culture. An explanation for the current results may be that angiotensin causes the release of undefined factors from cardiac myocytes, and that these secondary factors may be involved in either the activation of collagen gene transcription or in alteration of stability of collagen mRNA transcripts via a paracrine mechanism. Although our results indicate hemodynamic loading may potentiate the action of angiotensin, this scenario is unlikely as collagen type I gene expression was increased in the normotensive right ventricle.
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    Tamoxifen enhances interferon-regulated gene expression in breast cancer cells (1997)
    Springer
    Molecular and cellular biochemistry 167 (1997), S. 169-177 
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    ISSN: 1573-4919
    Keywords: tamoxifen ; interferon ; gene expression ; breast cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The molecular basis for the enhanced growth inhibition of MCF-7 human breast cancer xenografts by a combination of human interferon-β (IFN-β) and tamoxifen was investigated. Treatment of MCF-7, MDA-MB-231, and BT-20 cells with the combination of IFN-β and tamoxifen resulted in enhanced antiproliferative effects in vitro. Treatment with the combination of IFN-β and tamoxifen enhanced the expression of several IFN-β-inducible genes in human breast carcinoma cell lines relative to levels induced by IFN-β alone. Tamoxifen alone did not induce transcription of IFN-stimulated genes (ISGs). Augmentation of ISG expression by the combination of IFN-β and tamoxifen was noted in breast tumor cell lines irrespective of their functional estrogen receptor (ER) status or their dependence on estradiol for growth, suggesting that upregulation of ISGs was independent of ER status. Enhancement of IFN-stimulated gene expression by tamoxifen occurred at the transcripti onal level. Expression of transfected reporter genes under the control of IFN-α/β regulated promoters was also enhanced in IFN-β and tamoxifen-treated cells. Similarly, transcriptional induction of chimeric reporter plasmids driven by an IFN-γ inducible promoter (GAS; IFN-γ activated site) was also enhanced by the combination of IFN-γ and tamoxifen. In tamoxifen treated cells, IFN-β and IFN-γ readily activated transcription factors ISGF-3 and GAF, respectively. Therefore, augmentation of ISG expression by tamoxifen is an early event in the antitumoral activity of this drug combination. (Mol Cell Biochem 167: 169-177, 1997)
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    Use of a hammerhead ribozyme with cationic liposomes to reduce leukocyte type 12-lipoxygenase expression in vascular smooth muscle (1997)
    Springer
    Molecular and cellular biochemistry 172 (1997), S. 47-57 
    Details
    ISSN: 1573-4919
    Keywords: smooth muscle ; gene transfer ; DNA ; RNA ; ribozyme ; liposome ; lipoxygenase ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Chemically synthesized hammerhead-type ribozymes targeted against the porcine leukocyte-type 12-lipoxygenase (LO) have been developed and studied. One chimeric ribozyme consists of DNA in the non-enzymatic portions, and RNA in the enzymatic core as well as two phosphorothioate internucleotide linkages at 3′ terminus. The second ribozyme consists of ribonucleotide sequences generated by in vitro transcription. In this chapter we describe methodologies to first analyze the ribozyme catalytic activity in vitro by studying cleavage of target RNA in vitro. The subsequent sections will describe how to target the catalytic ribozyme and deliver it to porcine vascular smooth muscle cells (PVSMC) by a liposome-mediated method. Finally ways to evaluate its activity to inhibit expression of the 12-LO mRNA will be presented. These results demonstrate the feasibility of using ribozymes as novel candidates for therapeutic agents to block specific gene expression in vascular cells.
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    Construction of a human heart cDNA library and identification of cardiovascular based genes (CVBest) (1997)
    Springer
    Molecular and cellular biochemistry 172 (1997), S. 81-87 
    Details
    ISSN: 1573-4919
    Keywords: heart ; DNA ; library ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The availability of high quality cDNA libraries is often crucial to the successful identification and characterization of genes. The concepts and potential pitfalls of constructing cDNA libraries are presented. Various applications requiring high quality cDNA libraries are outlined, including large-scale single pass sequencing of cDNA clones to generate expressed sequence tags (ESTs) and differential screening of cDNA libraries. The usefulness of combining such approaches for the discovery of novel disease-related and cardiovascular-based ESTs (CVBest) is discussed.
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    Hydrogen peroxide-induced expression of the proto-oncogenes, c-jun, c-fos and c-myc in rabbit lens epithelial cells (1997)
    Springer
    Molecular and cellular biochemistry 173 (1997), S. 59-69 
    Details
    ISSN: 1573-4919
    Keywords: hydrogen peroxide ; oxidative stress ; gene expression ; lens epithelial cells ; N-acetylcysteine ; pyrrolidine dithiocarbamate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The involvement of H2O2 in cataract development has been established inboth human patients and animal models. At the molecular level H2O2 has beenobserved to cause damage to DNA, protein and lipid. To explore the oxidativestress response of the lens system at the gene expression level, we haveexamined the effects of H2O2 on the mRNA change of the proto-oncogenes,c-jun, c-fos and c-myc in a rabbit lens cell line, N/N1003A. H2O2 treatmentof the rabbit lens epithelial cells for 60 min induces quick up-regulationof both c-jun and c-fos mRNAs. The maximal induction is 38 fold for c-jun at150 µM and 72 fold for c-fos at 250 µM H2O2. Treatment ofN/N1003A cells with 50-250 µM H2O2 for 60 min leads to a 2-5 foldincrease of the c-myc mRNA level. H2O2 also induces an up-regulation intransactivity of the activating protein-1 (AP-1) as shown with a reportergene driven by a prolactin gene promoter with 4 copies of AP-1 binding sitesinserted in the upstream of the promoter. Maximal induction occurs with 150µM H2O2. In the same system, the antioxidants, N-acetyl-cysteine (NAC)and pyrrolidine dithiocarbamate (PDTC) at concentrations shown toup-regulate the mRNAs of both c-jun and c-fos, also enhance thetransactivity of AP-1. NAC and PDTC have different effects in modulating theinduction of AP-1 activity by H2O2 and TPA. These results reveal thatoxidative stress regulates expression of various regulatory genes in lenssystems, which likely affects cell proliferation, differentiation andviability and thus affect normal lens functions.
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    Molecular cloning of the cDNA coding for regucalcin and its mRNA expression in mouse liver: The expression is stimulated by calcium administration (1997)
    Springer
    Molecular and cellular biochemistry 173 (1997), S. 127-133 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; calcium-binding protein ; cDNA cloning ; gene expression ; mouse liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The molecular cloning of the cDNA coding for a Ca2+-binding proteinregucalcin and its mRNA expression in mouse liver were investigated. ThecDNA clone encoding a regucalcin was isolated from a mouse liver cDNAlibrary and sequenced. Analysis of the sequence of the cloned cDNA showedthat the cDNA encoded the complete amino acid sequence of the mouseregucalcin molecule; the cDNA had an open reading frame of 897 bp. Mouseregucalcin was composed of 299 amino acid residues, and its molecular weightwas estimated to be 33,406 Da. The amino acid sequence of mouse regucalcinhad 94% homology, as compared with that of rat regucalcin. Northernblot analysis with the mouse liver cDNA probe revealed that mouse regucalcinmRNA was mainly present in the liver but only slightly in the kidney with asize of 1.8 kb. Hepatic regucalcin mRNA level of male mouse was higher thanthat of female mouse. A single intraperitoneal administration of calciumchloride (5, 15, and 30 mg Ca2+/100 g body weight) to mice induced aremarkable increase in regucalcin mRNA in the liver; the increase inregucalcin mRNA levels at 30 min after calcium administration wasdose-dependent. The present results demonstrate that regucalcin mRNA in miceis uniquely expressed in the liver, and that its expression is stimulated bycalcium administration.
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    URL: http://dx.doi.org/10.1023/A:1006887929369
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    Cardiac hypertrophy: Old concepts, new perspectives (1997)
    Springer
    Molecular and cellular biochemistry 176 (1997), S. 273-279 
    Details
    ISSN: 1573-4919
    Keywords: cardiac hypertrophy ; myosin heavy chain ; gene expression ; adrenergic system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Growth of the heart in hypertrophy is accompanied by changes in the phenotypic expression of cardiac genes. To explore the molecular basis of cardiac hypertrophy, we have analyzed the regulation of myosin heavy chain gene (MHC) expression. In one set of experiments, pressure overload on the rat heart was produced by constriction of the abdominal aorta. Changes in the α and β-MHC mRNA were then studied in overloaded hearts and following load removal. Pressure overload resulted in down-regulation of the α-MHC with corresponding up-regulation of the steady state level of β-MHC mRNA. Load removal (debanding) resulted in regression of cardiac hypertrophy and a rapid return of α-MHC mRNA to normal values. In contrast, the recovery in β-MHC mRNA was much slower to the extent that it remained substantially elevated compared to respective sham controls even after 7 weeks of post-debanding. These results suggest that putative load-related signals independently regulate two genes. Several lines of evidence indicate that adrenergic nervous system plays an important role in the induction and maintenance of cardiac hypertrophy and in the redistribution of myosin isoforms. We have analyzed the effect of cAMP inducing agents on the regulation of a-MHC gene in primary cultures of the fetal (18 day) rat cardiac myocyte. Inclusion of 8 Br-cAMP in the culture media increased the expression of α-MHC promoter/reporter construct comprising of 2.9 kb upstream sequence of the α-MHC gene. Several deletion mutations in the α- MHC gene promoter defined the cAMP responsive boundaries to be a 32 bp region comprising of -71 to -40 bp sequences. Deletion of this region resulted in loss of cAMP response as well as in basal expression of α-MHC promoter/reporter construct. These data suggest a role of β-adrenergic pathway in the modulation of α-MHC gene expression.
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    URL: http://dx.doi.org/10.1023/A:1006865515646
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    Expression of calcium-binding protein regucalcin mRNA in the cloned rat hepatoma cells (Hr-II-E) is stimulated through Ca2+ signaling factors: Involvement of protein kinase C (1999)
    Springer
    Molecular and cellular biochemistry 198 (1999), S. 101-107 
    Details
    ISSN: 1573-4919
    Keywords: regucalcin ; Ca2+-binding protein ; protein kinase C ; Ca2+signaling ; gene expression ; H4-II-E hepatoma cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The expression of hepatic Ca2+-binding protein regucalcin in the cloned rat hepatoma cells (H4-II-E) was investigated. The change in regucalcin mRNA levels was analyzed by Northern blotting using rat liver regucalcin complementary DNA (0.9 kb of open reading frame). Regucalcin mRNA was expressed in H4-II-E hepatoma cells. This expression was clearly stimulated in the presence of serum (10% fetal bovine serum). Bay K 8644 (2. 5 × 10-6 M), a Ca2+ channel agonist, significantly stimulated regucalcin mRNA expression in the absence or presence of 10% serum. Dibutyryl cyclic AMP (10-3 M) did not have a stimulatory effect on the regucalcin mRNA expression. The presence of phorbol 12-myristate 13-acetate (PMA; 10-6 M) or estrogen (10-8 M) caused a significant increase in regucalcin mRNA levels in the hepatoma cells cultured in serum-free medium, while insulin (5 × 10-9 M) or dexamethasone (10-6 M) had no effect. Bay K 8644-stimulated regucalcin mRNA expression in the hepatoma cells was completely blocked in the presence of trifluoperazine (10-5 M), an antagonist of calmodulin, or staurosporine (10-7 M), an inhibitor of protein kinase C. The stimulatory effect of PMA was clearly inhibited in the presence of stauroporine. The present study demonstrates that regucalcin mRNA is expressed in the transformed H4-II-E hepatoma cells, and that the expression is stimulated through Ca2+-dependent signaling factors.
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    Posttranscriptional regulation of urokinase receptor gene expression in human lung carcinoma and mesothelioma cells in vitro (1999)
    Springer
    Molecular and cellular biochemistry 199 (1999), S. 189-200 
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    ISSN: 1573-4919
    Keywords: lung ; cancer ; urokinase ; receptor ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The urokinase-type plasminogen activator (uPA) interacts with its receptor (uPAR) to promote proteolysis as well as cell proliferation and migration. These functions contribute to the pathogenesis of neoplastic growth and invasiveness. Expression of uPAR in tumor extracts also inversely correlates with prognosis in many forms of cancer. In this study, we sought to determine if differences in uPAR expression were distinguishable between cultured human lung carcinoma and malignant mesothelioma subtypes. We also sought to determine if, as in malignant mesothelioma cells, uPAR expression is regulated at the posttranscriptional level in cultured malignant lung carcinoma cells. Using 125I-uPA binding and ligand blotting techniques, uPAR was expressed by phenotypically diverse lung carcinoma cell lines, including the H460, H157 and H1395 non-small cell lines and the H146 small cell lung carcinoma line. Increased uPAR expression was also detected in spindle-shaped (M33K) and epithelioid (M9K and MS-1) malignant mesothelioma cells. Selected mediators, including TGF-β, TNF-α, LPS and PMA, uniformly enhanced uPAR expression in each of the tumor cell lines. Steady state uPAR mRNA expression was determined by RNase protection assay and correlated directly with the changes in cell surface uPAR expression. By gel mobility shift and UV-cross linking assays, a uPAR mRNA binding protein (uPAR mRNABp) implicated in the posttranscriptional control of message stability, was identified in each of the cell lines. Expression of uPAR and its message in cultured lung carcinoma and malignant mesothelioma cells is similarly influenced by effectors present in the tumor microenvironment. Regulation of the uPAR message occurs at the posttranscriptional level in cultured small and non-small cell lung carcinoma cells as well as spindle-shaped and fibrous malignant mesothelioma cell lines. Posttranscriptional regulation of uPAR in all these cells involves the interaction of the uPAR mRNABp with uPAR mRNA, which promotes uPAR mRNA destabilization.
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    The Glycophorin A Gene Family in Gorillas: Structure, Expression, and Comparison with the Human and Chimpanzee Homologues (1997)
    Springer
    Biochemical genetics 35 (1997), S. 59-76 
    Details
    ISSN: 1573-4927
    Keywords: glycophorins ; gorilla ; evolution ; gene family ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Homologues of MN blood group antigens, encoded by members of the glycophorin A (GPA) gene family, are expressed in man, anthropoid apes, and some species of Old World monkeys. Previous studies had shown that a three-gene framework, most closely related to that in man, is present in the chimpanzee. Here we report the genomic structure, transcript map, and protein expression of the GYPA locus in gorillas. Compared to the corresponding human and chimpanzee homologues, gorilla GPA, GPB, and GPB/E genes each showed a high degree of sequence identity, with the same exon-intron organization. However, the expression of exons III, IV, or V encoding the extracellular or membrane domains of homologous glycophorins varied among the three species. Gorilla GPA and GPB/E genes were unique in that the former occurred in two allelic forms with or without the expression of exon III, whereas the latter contained one (ψ exon III) instead of two silenced exons (ψ exons III and IV). Differences from human but not chimpanzee GPA also included the presence of a hybrid M/N epitope and the absence of the sequon for N-glycosylation. Owing to the retention of a functional exon III, gorilla GPB was more similar to chimpanzee GPB than human GPB. A transspecies allele was identified in the gorilla that gave rise to the Henshaw (He)-like antigen similar to that found in man. These results provide further insight into the model for evolution of the GPA gene family, indicating that the mechanisms underlying inter- and intraspecific polymorphism of glycophorins could predate the divergence of gorillas as the consequence of gene duplication and diversification.
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    URL: http://dx.doi.org/10.1023/A:1022212630370
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    Novel methods for adenovirus-mediated gene transfer to blood vessels in vivo (1997)
    Springer
    Molecular and cellular biochemistry 172 (1997), S. 37-46 
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    ISSN: 1573-4919
    Keywords: gene transfer ; gene expression ; adenovirus ; blood vessel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Adenovirus-mediated gene transfer is a promising method for studies of vascular biology and potentially for gene therapy. Intravascular approaches for gene transfer to blood vessels in vivo generally require interruption of blood flow and have several limitations. We have used two alternative approaches for gene transfer to blood vessels in vivo using perivascular application of vectors. First, replication-deficient adenovirus expressing nuclear-targeted bacterial b-galactosidase was injected into cerebrospinal fluid via the cisterna magna of rats. Leptomeningeal cells over the major arteries were efficiently transfected, and adventitial cells of large vessels and smooth muscle cells of small vessels were occasionally stained. When viral suspension was injected with the rat in a lateral position, the reporter gene was expressed extensively on the ipsilateral surface of the brain. Thus, adenovirus injected into cerebrospinal fluid provides gene transfer in vivo to cerebral blood vessels and, with greater efficiency, to perivascular tissue. Furthermore, positioning of the head may ‘target’ specific regions of the brain. Second, vascular gene delivery was accomplished by perivascular injection of virus in peripheral vessels. Injection of the adenoviral vector within the periarterial sheath of monkeys resulted in gene transfer to the vessel wall that was substantial in magnitude although limited to cells in the adventitia. Approximately20% of adventitial cells expressed the transgene, with no gene transfer to cells in the intima or media. These approaches may provide alternative approaches for gene transfer to blood vessels, and may be useful for studies of vascular biology and perhaps vascular gene therapy.
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    URL: http://dx.doi.org/10.1023/A:1006803202179
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    Differential display: Identifying genes involved in cardiomyocyte proliferation (1997)
    Springer
    Molecular and cellular biochemistry 172 (1997), S. 111-120 
    Details
    ISSN: 1573-4919
    Keywords: differential display ; cardiac development ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract An estimated 15,000 different mRNA species are expressed in a typical mammalian cell. The differential expression of mRNAs in both a temporal and cell-specific manner determines the fate of the cell and creates the organism. Analysis of this differential gene expression has become a central aim of many laboratories attempting to understand the mechanisms underlying various biological processes. Currently, we are using a technique called differential display to analyze the differential expression of genes in cardiomyocytes. Differential display is a rapid and powerful technique that was introduced by Liang and Pardee in 1992. Since that time, it has been successfully applied by several groups, and it is quickly becoming a standard method for studying differential gene expression. Here, we present a detailed article discussing the differential display methodology and how we have utilized it to identify potential genes involved in cardiomyocyte proliferation. Furthermore, we have provided a list of materials and supplied examples of data obtained, in an effort to allow the reader to perform the technique with success in their own laboratory.
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    URL: http://dx.doi.org/10.1023/A:1006819705814
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    Increased gene expression of plasminogen activators and inhibitors in left ventricular hypertrophy (1997)
    Springer
    Molecular and cellular biochemistry 176 (1997), S. 265-271 
    Details
    ISSN: 1573-4919
    Keywords: plasminogen activators ; plasminogen activator inhibitors ; gene expression ; left ventricular hypertrophy ; pressure overload
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract In the early stages of left ventricular hypertrophy (LVH) acute adaptive changes occur in the coronary vasculature as it remodels. Plasminogen activators (PAs) and inhibitors (PAIs) have the potential effects of proteolytic degradation that is relevant to tissue remodeling and angiogenesis. Our study focused on the possible roles of PAI-1, PAI-2, uPA and tPA in myocyte hypertrophy and angiogenesis in the early and late stages of pressure overload induced left ventricular hypertrophy (LVH). We divided seventeen adult swine, weighing 24.2 ± 6.5 kg, into four groups: control, sham-operated, early LVH and late heart failure LVH group. At surgery we placed a fixed constrictor on the ascending aorta immediately above the aortic valve. This increased LV systolic pressure from 133 ± 15 to 193 ± 24 mm Hg after the surgery. We subdivided the early group into groups of 3 animals each that we euthanized at 8, 24 and 72 h after operation and obtained heart samples for analysis. In the late heart failure group individual animals were euthanized at 55, 59, 62 and 72 days after the detection of congestive heart failure. We also obtained tissue samples from the control and sham-operated swine. Sections for histologic analysis were fixed in 10% buffered formalin. We isolated RNA, size fractionated it using 1% formaldehyde-agarose gel electrophoresis and then did Northern blots. The mRNAs from both PAI-1 and PAI-2 showed a remarkable increase at 8 and 24 h after acute aortic constriction and returned to control by 72 h. Regional differences showed that most of the increases were in the endocardium. Three animals in the late heart failure LVH group were determined to be in congestive heart failure at about 2 months after the onset of aortic constriction. In these animals PAI-1 and PAI-2 were increased in both the left and right ventricles but remained low in an animal of the same elevation in aortic pressure seen by the LV who did not have congestive failure. These data suggest that PA and PAI gene expressions change before morphologic changes occur in the early stages of developing LVH. Also at the time of onset of congestive heart failure this increased expression reappears. PAs and PA inhibitors mRNA levels vary in the different regions of the heart reflecting changing wall stresses. Thus, the PAs and PA inhibitors may play an important role in angiogenesis that occurs during the early stages of LVH. The increased expression in the late stage of LVH may reflect further changes in wall stresses since these animals also showed overt clinical signs of heart failure.
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    URL: http://dx.doi.org/10.1023/A:1006813531576
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    Protein tyrosine phosphatase gene expression analysis in Swiss 3T3 fibroblasts (1998)
    Springer
    Molecular and cellular biochemistry 178 (1998), S. 157-162 
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    ISSN: 1573-4919
    Keywords: protein tyrosine phosphatases ; gene expression ; degenerate deoxyoligonucleotides ; RT-PCR ; Swiss 3T3 fibroblasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The aim of this study was to identify protein tyrosine phosphatases (PTPs) expressed in Swiss 3T3 fibroblasts and to examine their expression levels as well as to characterize quantitative aspects of RT-PCR based on degenerate deoxyoligonucleotides. By using an RT-PCR assay based on degenerate deoxyoligonucleotide primers, expression of mRNAs for two cytoplasmic- and six transmembrane-type PTPs in Swiss 3T3 cells was detected. The sequences of two of them are new. Among nine analyzed PTPs expressed to widely varied extends, only three have mRNA levels high enough to be seen on Northern blots with 10 µg of total RNA per lane. The frequencies with which the examined PTPs are represented among the PCR amplification products, correlate stronger with the primer fidelity, defined as the number of mismatches between the primer- and the cDNA target-sequences, rather than with the PTP expression levels. In conclusion, an RT-PCR assay based on degenerate primers can be successfully used to sample the expressed PTPs and to identify new members of this gene family. However, reliable quantification of their mRNA levels can only be achieved using the classical approaches, like Northern, RNase protection assay or non-degenerate quantitative RT-PCR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006897629337
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