ALBERT

Beschreibung: Nell’ambito del progetto EDISECUR, finanziato della regione Lazio, è stato sviluppato un prototipo di telesensore infrasonico, TIS, a tracciamento di speckle per la misura della velocità angolare, delle frequenze fondamentali e delle armoniche di una superficie sottoposta a oscillazioni. Il TIS si presta particolarmente per il rilevamento e il monitoraggio nel tempo degli edifici, ponti e altri manufatti. Conoscere lo stato vibrazionale di queste strutture può essere d’interesse sia per la loro caratterizzazione dinamica che per la sicurezza. Questo prototipo, sufficientemente compatto, si presta a una misura immediata della velocità angolare e, con semplici operazioni, si possono dedurre spostamenti e accelerazioni angolari. Dalle grandezze angolari e dalla conoscenza geometrica della superficie, tramite alcuni schemi ed esempi, si mostra come sia possibile determinare anche altri parametri cinematici lineari. Il telesensore può essere impiegato nelle misure delle vibrazioni di superfici a lunga distanza di varia natura, anche laddove non fosse possibile accedere per eseguire una misura diretta. Lo strumento copre un campo di frequenze fino alle decine di Hz, ha una sensibilità e una dinamica tale da rilevare le vibrazioni indotte dal rumore industriale, dal traffico, dal vento e altro. Questo lavoro è principalmente rivolto alle applicazioni del TIS nel rilevamento delle vibrazioni delle strutture ed è in questo ambito che vengono spiegate le modalità, i limiti e i vantaggi del suo impiego insieme agli errori insiti nella tecnica di misura. Dato che il TIS misura un movimento relativo tra lo stesso strumento e la superficiebersaglio, sono stati valutati gli errori delle vibrazioni dovute alla microsismicità e altre cause ambientali. Vengono infine riportati due preliminari esempi di misura su una struttura edile.

Beschreibung: Regione Lazio, progetto EDISECUR

Beschreibung: Published

Beschreibung: 1-36

Beschreibung: 7TM.Sviluppo e Trasferimento Tecnologico

Beschreibung: JCR Journal

Beschreibung: L’Istituto Nazionale di Geofisica e Vulcanologia (INGV) riceve, nella Sala di Sorveglianza Sismica e Centro Allerta Tsunami di Roma, i segnali in tempo reale da centinaia di stazioni sismiche distribuite sul territorio nazionale. Entro due minuti dall’occorrenza di un qualsiasi terremoto, appositi sistemi automatici forniscono una prima valutazione dei parametri ipocentrali. Due sismologi, sempre presenti nella sala operativa della sede centrale, controllano le informazioni ottenute e, per i terremoti sopra una determinata soglia di magnitudo (ML ≥ 2.5), comunicano alla Sala Situazione Italia della Protezione Civile i dati elaborati, in media in circa 12 minuti (massimo entro 30 minuti) [Margheriti et al., 2021]. La valutazione definitiva dei parametri ipocentrali di tutti i terremoti, dai più grandi avvertiti in vaste aree del territorio ai più piccoli rilevati solo da pochi strumenti, è demandata a un’analisi più accurata svolta in un secondo tempo, ormai da alcuni decenni, da un gruppo di analisti specializzati nell’interpretazione dei segnali sismici. Gli analisti sismologi del Bollettino Sismico Italiano revisionano tutti i dati registrati dalle stazioni della Rete Sismica Nazionale (RSN) dell’INGV e riconoscono la presenza di terremoti attraverso un’analisi diretta delle forme d’onda. In tal modo l’analista rileva il tempo d’arrivo delle onde sismiche ai vari sensori e valuta l’ampiezza delle oscillazioni e la direzione del moto del suolo; questi parametri, utilizzati da apposite procedure di calcolo, consentono di localizzare ogni terremoto e di valutare la magnitudo associata. Le informazioni così ottenute confluiscono nel database che l’INGV gestisce e che mette a disposizione della comunità1. Questa pubblicazione ha come scopo quello di far conoscere un prodotto dell’Istituto Nazionale di Geofisica e Vulcanologia, Il Bollettino Sismico Italiano (BSI), con particolare riferimento all’anno 2015. Saranno delineate le principali caratteristiche della sismicità naturale e quella di origine antropica registrata in Italia nel corso dell’anno esaminato.

Beschreibung: Published

Beschreibung: 1-48

Beschreibung: 4IT. Banche dati

Beschreibung: JCR Journal

Beschreibung: Crystalline rocks can produce dangerous radiation levels on the basis of their content in radioisotopes. Here, we report radiological data from 10 metamorphic and igneous rock samples collected from the crystalline basement of the Peloritani Mountains (southern Italy). In order to evaluate the radiological properties of these rocks, the gamma radiation and the radon emanation have been measured. Moreover, since some of these rocks are employed as building materials, we assess the potential hazard for population connected to their use. Gamma spectroscopy was used to measure the 226Ra, 232Th and 40K activity concentration, whereas the radon emanation was investigated by using a RAD 7 detector. The results show 226Ra, 232Th and 40K activity concentration values ranging from (17 ± 4) to (56 ± 8) Bq kg-1, (14 ± 3) to (77 ± 14) Bq kg-1 and (167 ± 84) to (1760 ± 242) Bq kg-1, respectively. Values of the annual effective dose equivalent outdoor range from 0.035 to 0.152 mSv y-1, whereas the gamma index is in the range of 0.22-0.98. The 222Rn emanation coefficient and the 222Rn surface exhalation rate vary from (0.63 ± 0.3) to (8.27 ± 1.6)% and from (0.12 ± 0.03) to (2.75 ± 0.17) Bq m-2 h-1, respectively. The indoor radon derived from the building use of these rocks induces an approximate contribution to the annual effective dose ranging from 8 to 176 μSv y-1. All the obtained results suggest that the crystalline rocks from the Peloritani Mountains are not harmful for the residential population, even though they induce annual effective doses due to terrestrial gamma radiation above the worldwide average values. Moreover, their use as building materials does not produce significant health hazards connected to the indoor radon exposure.

Beschreibung: Published

Beschreibung: 452–464

Beschreibung: 6A. Geochimica per l'ambiente e geologia medica

Beschreibung: JCR Journal

Beschreibung: The Pollino range is a region of slow deformation where earthquakes generally nucleate on low-angle normal faults. Recent studies have mapped fault structures and identified fluid related dynamics responsible for historical and recent seismicity in the area. Here, we apply the coda-normalization method at multiple frequencies and scales to image the 3-D P-wave attenuation (QP) properties of its slowly deforming fault network. The wide-scale average attenuation properties of the Pollino range are typical for a stable continental block, with a dependence of QP on frequency of Q−1 P = (0.0011   0.0008) f (0.36 0.32). Using only waveforms comprised in the area of seismic swarms, the dependence of attenuation on frequency increases [Q−1 P = (0.0373   0.0011) f (−0.59 0.01)], as expected when targeting seismically active faults. A shallow very-low-attenuation anomaly (max depth of 4–5 km) caps the seismicity recorded within the western cluster 1 of the Pollino seismic sequence (2012, maximum magnitude Mw = 5.1). High-attenuation volumes below this anomaly are likely related to fluid storage and comprise the western and northern portions of cluster 1 and the Mercure basin. These anomalies are constrained to the NW by a sharp low-attenuation interface, corresponding to the transition towards the eastern unit of the Apennine Platform under the Lauria mountains. The low-seismicity volume between cluster 1 and cluster 2 (maximum magnitude Mw = 4.3, east of the primary) shows diffuse low-to-average attenuation features. There is no clear indication of fluid-filled pathways between the two clusters resolvable at our resolution. In this volume, the attenuation values are anyway lower than in recognized low-attenuation blocks, like the Lauria Mountain and Pollino Range. As the volume develops in a region marked at surface by small-scale cross-faulting, it suggests no actual barrier between clusters, more likely a system of small locked fault patches that can break in the future. Our model loses resolution at depth, but it can still resolve a 5-to-15-km-deep high-attenuation anomaly that underlies the Castrovillari basin. This anomaly is an ideal deep source for the SE-to-NW migration of historical seismicity. Our novel deep structural maps support the hypothesis that the Pollino sequence has been caused by a mechanism of deep and lateral fluid-induced migration.

Beschreibung: Natural Environment Research Council (NERC) Centre for Doctoral Training (CDT) in Oil and Gas. University of Aberdeen.

Beschreibung: Published

Beschreibung: 536–547

Beschreibung: 4T. Sismicità dell'Italia

Beschreibung: JCR Journal

Beschreibung: This study represents the first attempt to combine the geomorphological characteristics of the island of Ustica with the human settlements that have been established during prehistory, with the purpose of reconstructing the interactions between communities and the natural environment from the Neolithic to the Middle Bronze Age (6th - 1st millennia B.C.). Ustica is a small island in the Southern Tyrrhenian Sea, visible but far (~55 km) from the northern coast of western Sicily. Its rugged volcanic nature, remodeled and enriched by the sea, offered to the first colonizers a wide repertoire of opportunities and challenges. This island can be treated as an ideal “laboratory” to understand how settlers, taking their first steps towards the foundation of organized communities, were able to seize opportunities or succumb to obstacles. The review of archaeological research until now carried out in Ustica, integrated with geomorphological data and other biogeographical indicators, offers a picture of the prehistory of Ustica in which human presence is continuous and distributed in various sites of the island characterized by different physiographic characteristics. There are phases dominated by the choice of naturally protected sites and phases in which settlements expands on open land, suitable for agricultural use. Where the archaeological evidence is scarce, the geomorphological peculiarities allow us to decipher the vocations and characters of a human settlement. The study leads to an open question: in the Middle Bronze Age, after about five thousand years of uninterrupted habitation of Ustica, which factors, geological, social, or other, induced the early communities to abandon the island, without returning there for about eight centuries, until the Hellenistic-Roman age?

Beschreibung: Published

Beschreibung: VO550

Beschreibung: 6A. Geochimica per l'ambiente e geologia medica

Beschreibung: JCR Journal

Beschreibung: This work sets out to identify a state-of-the-art system to be used for the calibration of seismic sensors. The aim is to acquire such a system within the framework of the PON ARS01 00926 EWAS (an Early Warning System for cultural heritage) project, which seeks to develop new technologies for the protection, conservation and safety of cultural heritage and envisages creating a newly developed seismic monitoring system. This system will exploit the ETL3D/5s-H hybrid sensors, resulting from the integration of a precision accelerometer within the ETL3D/5s velocimeter [Fertitta et al., 2020]. The new calibration system, already acquired and being installed, can be used by the EWAS project partners (including the National Institute of Geophysics and Volcanology and the Kore University of Enna), to calibrate the ETL3D/5s-H sensors, and by external organisations to calibrate or gauge other seismic sensors, thus providing a useful service to the scientific community and supporting industrial activities. This paper presents the method used and the activities undertaken to define the technical specifications of the calibration system. A feasibility study of an electromechanical vibrating table and the testing of two electrodynamic calibration systems were carried out. One of the electrodynamic systems is the CS18P (Calibration System for Seismic Sensors) produced by the German firm SPEKTRA. The CS18P comprises two vibrating tables, one horizontal and one vertical, which, thanks to their fluid-dynamic suspension, eliminate the sliding and rolling friction associated with the movement of the moving part with respect to the fixed part. A hardware and software system monitors and controls the motion in real time, analyses the data and automatically processes a predefined set of measurements. In the light of the technical specifications and experimental results, the CS18P represents the ideal solution for the aims of the EWAS project and also in view of the possible future uses of the calibration system.

Beschreibung: Published

Beschreibung: 1-38

Beschreibung: 2IT. Laboratori analitici e sperimentali

Beschreibung: JCR Journal

Beschreibung: In recent years, new approaches for developing earthquake rupture forecasts (ERFs) have been proposed to be used as an input for probabilistic seismic hazard assessment (PSHA). Zone- based approaches with seismicity rates derived from earthquake catalogs are commonly used in many countries as the standard for national seismic hazard models. In Italy, a single zone- based ERF is currently the basis for the official seismic hazard model. In this contribution, we present eleven new ERFs, including five zone-based, two smoothed seismicity-based, two fault- based, and two geodetic-based, used for a new PSH model in Italy. The ERFs were tested against observed seismicity and were subject to an elicitation procedure by a panel of PSHA experts to verify the scientific robustness and consistency of the forecasts with respect to the observations. Tests and elicitation were finalized to weight the ERFs. The results show a good response to the new inputs to observed seismicity in the last few centuries. The entire approach was a first attempt to build a community-based set of ERFs for an Italian PSHA model. The project involved a large number of seismic hazard practitioners, with their knowledge and experience, and the development of different models to capture and explore a large range of epistemic uncertainties in building ERFs, and represents an important step forward for the new national seismic hazard model.

Beschreibung: Published

Beschreibung: SE220

Beschreibung: 6T. Studi di pericolosità sismica e da maremoto

Beschreibung: JCR Journal

Beschreibung: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Yoshii, A., & Green, W. N. Editorial: role of protein palmitoylation in synaptic plasticity and neuronal differentiation. Frontiers in Synaptic Neuroscience, 12(27), (2020), doi:10.3389/fnsyn.2020.00027.

Beschreibung: Protein palmitoylation, the reversible addition of palmitate to proteins, is a dynamic post-translational modification. Both membrane (e.g., channels, transporters, and receptors) and cytoplasmic proteins (e.g., cell adhesion, scaffolding, cytoskeletal, and signaling molecules) are substrates. In mammals, palmitoylation is mediated by 23-24 palmitoyl acyltransferases (PATs), also called ZDHHCs for their catalytic aspartate-histidine-histidine-cysteine (DHCC) domain. PATs are integral membrane proteins found in cellular membranes. In the palmitoylation cycle, palmitate is removed by the depalmitoylation enzymes, acyl palmitoyl transferases (APT1 and 2), and α/β Hydrolase domain-containing protein 17 (ABHD17A-C). These are cytoplasmic proteins that are targeted to membranes where they are substrates for PATs. The second class of depalmitoylating enzymes are palmitoyl thioesterases, PPT1 and 2, discovered through their association with infantile neuronal ceroid lipofuscinosis. These are secreted proteins found in the lumen of intracellular organelles, primarily lysosomes, where their function as depalmitoylating enzymes is unclear.

Beschreibung: This work was supported by University of Illinois start-up fund (to AY) and NIH/NIDA (grant DA044760 to WG).

Beschreibung: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Carroll, E. L., Ott, P. H., McMillan, L. F., Galletti Vernazzani, B., Neveceralova, P., Vermeulen, E., Gaggiotti, O. E., Andriolo, A., Baker, C. S., Bamford, C., Best, P., Cabrera, E., Calderan, S., Chirife, A., Fewster, R. M., Flores, P. A. C., Frasier, T., Freitas, T. R. O., Groch, K., Hulva, P., Kennedy, A., Leaper, R., Leslie, M. S., Moore, M., Oliveira, L., Seger, J., Stepien, E. N., Valenzuela, L. O., Zerbini, A., & Jackson, J. A. Genetic diversity and connectivity of southern right whales (Eubalaena australis) found in the Brazil and Chile-Peru wintering grounds and the South Georgia (Islas Georgias del Sur) feeding ground. Journal of Heredity, 111(3), (2020): 263-276, doi:10.1093/jhered/esaa010.

Beschreibung: As species recover from exploitation, continued assessments of connectivity and population structure are warranted to provide information for conservation and management. This is particularly true in species with high dispersal capacity, such as migratory whales, where patterns of connectivity could change rapidly. Here we build on a previous long-term, large-scale collaboration on southern right whales (Eubalaena australis) to combine new (nnew) and published (npub) mitochondrial (mtDNA) and microsatellite genetic data from all major wintering grounds and, uniquely, the South Georgia (Islas Georgias del Sur: SG) feeding grounds. Specifically, we include data from Argentina (npub mtDNA/microsatellite = 208/46), Brazil (nnew mtDNA/microsatellite = 50/50), South Africa (nnew mtDNA/microsatellite = 66/77, npub mtDNA/microsatellite = 350/47), Chile–Peru (nnew mtDNA/microsatellite = 1/1), the Indo-Pacific (npub mtDNA/microsatellite = 769/126), and SG (npub mtDNA/microsatellite = 8/0, nnew mtDNA/microsatellite = 3/11) to investigate the position of previously unstudied habitats in the migratory network: Brazil, SG, and Chile–Peru. These new genetic data show connectivity between Brazil and Argentina, exemplified by weak genetic differentiation and the movement of 1 genetically identified individual between the South American grounds. The single sample from Chile–Peru had an mtDNA haplotype previously only observed in the Indo-Pacific and had a nuclear genotype that appeared admixed between the Indo-Pacific and South Atlantic, based on genetic clustering and assignment algorithms. The SG samples were clearly South Atlantic and were more similar to the South American than the South African wintering grounds. This study highlights how international collaborations are critical to provide context for emerging or recovering regions, like the SG feeding ground, as well as those that remain critically endangered, such as Chile–Peru.

Beschreibung: This work was supported by the EU BEST 2.0 medium grant 1594 and UK DARWIN PLUS grant 057 and additional funding from the World Wildlife Fund GB107301. The collection of the Chile–Peru sample was supported by the Global Greengrants Fund and the Pacific Whale Foundation. The collection of the Brazilian samples was supported through grants by the Brazilian National Research Council to Paulo H. Ott (CNPq proc. n° 144064/98-7) and Paulo A.C. Flores (CNPq proc. n° 146609/1999-9) and with support from the World Wildlife Fund (WWF-Brazil). The collection of the South African samples was supported by the Global Greengrants Fund, the Pacific Whale Foundation and Charles University Grant Agency (1140217). E.L.C. was partially supported by a Rutherford Discovery Fellowship from the Royal Society of New Zealand. This study forms part of the Ecosystems component of the British Antarctic Survey Polar Sciences for Planet Earth Programme, funded by the Natural Environment Research Council.

Beschreibung: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Beam, J. P., Becraft, E. D., Brown, J. M., Schulz, F., Jarett, J. K., Bezuidt, O., Poulton, N. J., Clark, K., Dunfield, P. F., Ravin, N. V., Spear, J. R., Hedlund, B. P., Kormas, K. A., Sievert, S. M., Elshahed, M. S., Barton, H. A., Stott, M. B., Eisen, J. A., Moser, D. P., Onstott, T. C., Woyke, T., & Stepanauskas, R. Ancestral absence of electron transport chains in Patescibacteria and DPANN. Frontiers in Microbiology, 11, (2020): 1848, doi:10.3389/fmicb.2020.01848.

Beschreibung: Recent discoveries suggest that the candidate superphyla Patescibacteria and DPANN constitute a large fraction of the phylogenetic diversity of Bacteria and Archaea. Their small genomes and limited coding potential have been hypothesized to be ancestral adaptations to obligate symbiotic lifestyles. To test this hypothesis, we performed cell–cell association, genomic, and phylogenetic analyses on 4,829 individual cells of Bacteria and Archaea from 46 globally distributed surface and subsurface field samples. This confirmed the ubiquity and abundance of Patescibacteria and DPANN in subsurface environments, the small size of their genomes and cells, and the divergence of their gene content from other Bacteria and Archaea. Our analyses suggest that most Patescibacteria and DPANN in the studied subsurface environments do not form specific physical associations with other microorganisms. These data also suggest that their unusual genomic features and prevalent auxotrophies may be a result of ancestral, minimal cellular energy transduction mechanisms that lack respiration, thus relying solely on fermentation for energy conservation.

Beschreibung: This work was funded by the USA National Science Foundation grants 1441717, 1826734, and 1335810 (to RS); and 1460861 (REU site at Bigelow Laboratory for Ocean Sciences). RS was also supported by the Simons Foundation grant 510023. TW, FS, and JJ were funded by the U.S. Department of Energy Joint Genome Institute, a DOE Office of Science User Facility supported under Contract No. DE-AC02-05CH11231. NR group was funded by the Russian Science Foundation (grant 19-14-00245). SS was funded by USA National Science Foundation grants OCE-0452333 and OCE-1136727. BH was funded by NASA Exobiology grant 80NSSC17K0548.

Beschreibung: This article has been accepted for publication in Geophysical Journal International ©: The Authors 2021. Published by Oxford University Press on behalf of the Royal Astronomical Society. All rights reserved. Uploaded in accordance with the publisher's self-archiving policy.

Beschreibung: In a recent study (Jozinovi\'c et al, 2020) we showed that convolutional neural networks (CNNs) applied to network seismic traces can be used for rapid prediction of earthquake peak ground motion intensity measures (IMs) at distant stations using only recordings from stations near the epicenter. The predictions are made without any previous knowledge concerning the earthquake location and magnitude. This approach differs from the standard procedure adopted by earthquake early warning systems (EEWSs) that rely on location and magnitude information. In the previous study, we used 10 s, raw, multistation waveforms for the 2016 earthquake sequence in central Italy for 915 events (CI dataset). The CI dataset has a large number of spatially concentrated earthquakes and a dense station network. In this work, we applied the CNN model to an area around the VIRGO gravitational waves observatory sited near Pisa, Italy. In our initial application of the technique, we used a dataset consisting of 266 earthquakes recorded by 39 stations. We found that the CNN model trained using this smaller dataset performed worse compared to the results presented in the original study by Jozinovi\'c et al. (2020). To counter the lack of data, we adopted transfer learning (TL) using two approaches: first, by using a pre-trained model built on the CI dataset and, next, by using a pre-trained model built on a different (seismological) problem that has a larger dataset available for training. We show that the use of TL improves the results in terms of outliers, bias, and variability of the residuals between predicted and true IMs values. We also demonstrate that adding knowledge of station positions as an additional layer in the neural network improves the results. The possible use for EEW is demonstrated by the times for the warnings that would be received at the station PII.

Beschreibung: RISE (Union's Horizon 2020 research and innovation programme, grant agreement No.821115)

Beschreibung: Published

Beschreibung: 704–718

Beschreibung: 5T. Sismologia, geofisica e geologia per l'ingegneria sismica

Beschreibung: JCR Journal

Beschreibung: Mobile network routers in seismic and volcanic surveillance

Beschreibung: Published

Beschreibung: 1-36

Beschreibung: 1IT. Reti di monitoraggio e sorveglianza

Beschreibung: N/A or not JCR

Beschreibung: This special issue of “Annals of Geophysics” concerns the dissemination of knowledge on the prevention of damage mainly due to non-structural elements during earthquakes and its practical application at houses, schools and offices by common citizens, companies and institutions. The seismic capacity of buildings and other civil engineering structures and infrastructures are object of regulations for design and construction, and in some cases also the design, fabrication and mounting of electrical and mechanical equipments. Consequently, even in strong earthquakes many collapses of buildings and infrastructures are avoided. However, with few exceptions, design procedures do not aim at avoiding seismic vibrations from being transferred to the structures, but enable the structures to resist to the effects of those vibrations

Beschreibung: European Commission’s Humanitarian Aid and Civil Protection (Grant agreement ECHO/SUB/2015/718655/PREV28)

Beschreibung: Published

Beschreibung: SE331

Beschreibung: 3TM. Comunicazione

Beschreibung: JCR Journal

Beschreibung: Encouraging property owners and individuals to adopt mitigation measures to improve the resilience of their buildings and equipments to seismic hazard has been a major challenge in many earthquake- prone countries. Few business leaders are aware of the fragility of their supply chains or other critical systems due to earthquake hazard. Bridging the gap between research production and research use is another crucial challenge for the earthquake risk research process. The KnowRISK project outcome is aimed at encouraging the proactive engagement of multi- stakeholders (community at large, schools, business community and local govern-ment groups) undertaking non-structural mitigation measures that will minimize earthquake losses to individuals and communities. Engaging stakeholders, taking into account their needs and inputs to maintain critical and urgent business activities, can contribute to the research findings and ensure that our data collection is thorough and complete. Engagement with stakeholders, during the whole process can lead to improved outcomes and for the development of viable solutions, for business and society, because of stakeholder’s role and influence within the organizations.

Beschreibung: European Commission’s Humanitarian Aid and Civil Protection (Grant agreement ECHO/SUB/2015/718655/PREV28)

Beschreibung: Published

Beschreibung: SE324

Beschreibung: 5T. Sismologia, geofisica e geologia per l'ingegneria sismica

Beschreibung: JCR Journal

Beschreibung: The focus of this study is the analysis of a cave in Central Italy, the Beatrice Cenci cave, in order topoint out and constrain evidence of possible past earthquakes and of fault activity in the area. Weperformed a survey of seismic related damages within the cave. This included the analysis ofbroken/collapsed speleothems, the recognition of structural collapse, of tilting/growth alteration inthe speleothems, and the mapping of fractures, joints and/or faults. To timely set the occurrence ofthe recognized damage, organic sediments were dated with 14C radiocarbon method. The resultsmerged toward the recognition of two distinct seismic shaking events affecting the caveenvironment, one older than 30 kyr and another around 7 kyr. The deformation observed withinthe cave led us to the hypothesis that the events of damage were possibly linked to the activity ofthe regional tectonic lineament that crosses the cave, i.e., the Liri normal fault. The morphology andthe evolution of the cave appear controlled by the fault zone. These speleoseismological resultsprovided a new contribution on the knowledge of the past activity of the Liri fault and on theearthquake history of this sector of Central Apennines.

Beschreibung: INGV

Beschreibung: Published

Beschreibung: SE435

Beschreibung: 2T. Deformazione crostale attiva

Beschreibung: JCR Journal

Beschreibung: Il presente lavoro raccoglie alcune esperienze di Progetti di Alternanza Scuola Lavoro (d’ora in poi ASL) realizzati dall’INGV negli anni scolastici dal 2015 al 2019 e dedicati al rischio sismico, alle scienze polari ed agli strumenti per la divulgazione scientifica. Le esperienze descrivono il lavoro realizzato da un gruppo di ricercatori INGV con competenze in diversi ambiti disciplinari (sismologia, geologia, vulcanologia fisica dell’atmosfera, psicologia) e in alcuni casi le attività sono state svolte in collaborazione con ricercatori di altri enti. Il gruppo di lavoro è composto da ricercatori che da molti anni dedicano parte del loro tempo-lavoro alle attività di divulgazione scientifica, ai progetti educativi, alla didattica della scienza. In una parola a quella che oggi rappresenta, per gli enti di ricerca, la “Terza missione”, ovvero l’insieme di tutte le attività finalizzate a creare una connessione bidirezionale tra il mondo della ricerca e la società. Ciò che ha caratterizzato l’approccio dei ricercatori INGV ai progetti ASL, sia nella fase di progettazione che di realizzazione, è la finalità di consentire agli studenti di vivere un’esperienza reale ed immersiva in un contesto di lavoro. E quindi l’opportunità di sperimentare capacità e abilità che caratterizzano il contesto organizzativo (diritti, doveri, responsabilità, impegni, regole), diversamente dal mondo della scuola. Il secondo aspetto al quale ci si è riferiti nella realizzazione dei progetti è quello di consentire agli studenti di esplorare, in particolare il contesto delle attività lavorative connesse al mondo della ricerca. Da questo punto di vista gli studenti hanno potuto acquisire conoscenze in ambiti tematici specifici, hanno avuto modo di sperimentare metodi e tecniche proprie del mondo della ricerca e di acquisire capacità e abilità trasversali come il lavoro in gruppo. Il contributo è organizzato in tre capitoli in relazione ai temi affrontati nei progetti. Nel primo sono riportate le schede dei tre progetti dedicate al tema Terremoto; nel secondo vengono descritti i due progetti dedicati alla Divulgazione Scientifica e nel terzo un progetto sulle Scienze Polari. Per rendere maggiormente fruibile la lettura, ogni progetto viene descritto attraverso una scheda sintetica che evidenza le caratteristiche principali: titolo, referenti INGV, studenti partecipanti, periodo, descrizione delle attività, obiettivi e considerazioni sull’esperienza. Nel capitolo conclusivo viene proposto un bilancio delle esperienze realizzate, evidenziando punti di forza e aree di miglioramento dei progetti, con la finalità di condividere suggerimenti ed idee per tracciare percorsi formativi maggiormente efficaci nel futuro.

Beschreibung: Published

Beschreibung: 119-131

Beschreibung: 2TM. Divulgazione Scientifica

Beschreibung: N/A or not JCR

Beschreibung: “Move, protect, secure” were the three key points that the KnowRISK (Know your city, Reduce seISmic risK through non-structural elements) project posed at the core of its communication and dissemination strategy. This three key points enable each person, professional or not, to reduce non-structural damage caused by earthquakes. Dissemination is usually the last but never the least step of a communication plan, and indeed it played a crucial role in KnowRISK project for conveying the three key-point message to the widest audience. Standard dissemination activities, such as open-door events, and internet allowed us to achieve a wide spreading of ideas and best practices, reaching more than 4,000 non-professionals and almost 50,000 page views of the KnowRISK project website (in three years), respectively. As communication was recipient-targeted, the dissemination task of the project was addressed to professionals, layman, and schools. In particular, schools were chosen in order to profit from the chain-reaction action that is capable to spread a message from students to the surrounding environment.

Beschreibung: This study was co-financed by the European Commission’s Humanitarian Aid and Civil Protection through the European project KnowRISK (Know your city, Reduce seISmic risK through non-structural elements; Grant agreement ECHO/SUB/2015/718655/PREV28).

Beschreibung: Published

Beschreibung: SE328

Beschreibung: 6T. Studi di pericolosità sismica e da maremoto

Beschreibung: JCR Journal

Beschreibung: We present the commented transcription of three rare articles published in 19091910 by Father Atto Maccioni o.f.m. This friar is one of the least known among the many ecclesiastics who cultivated seismology from the 18th century onwards. Maccioni, from 1908 to 1926, managed a seismic observatory at the Sienese convent of the Osservanza. He devised an “Avvisatore”: a device equipped with a modified coherer with respect to those adopted in radiotelegraphic communications, aimed to detect natural radio signals associated with earthquakes. From the few available results it seems that the scholar obtained positive feedback, but the most interesting aspect of the story is that until now it was generally believed that this kind of research began only quite recently [Warwick, 1982]. On the contrary Maccioni may have been the first ever to study a possible electromagnetic precursor, more than a century ago.

Beschreibung: Presentiamo la trascrizione commentata di tre rari articoli pubblicati nel 19091910 da padre Atto Maccioni o.f.m., uno dei meno noti tra i tanti ecclesiastici che si sono occupati di sismologia dal XVIII secolo in poi. Maccioni, che dal 1908 al 1926 gestì un osservatorio sismico presso il convento senese dell’Osservanza, aveva ideato l’Avvisatore, un dispositivo munito di un coherer diverso da quelli radiotelegrafici e destinato a rilevare segnali radio naturali associati ai terremoti. Dai pochi risultati disponibili sembra che lo studioso abbia avuto dei riscontri positivi ma l’aspetto più interessante della vicenda è che finora si era creduto che ricerche di questo tipo fossero iniziate solo molto di recente [Warwick, 1982]. Invece Maccioni potrebbe essere stato il primo al mondo a studiare, più di un secolo fa, un ipotetico precursore elettromagnetico.

Beschreibung: Published

Beschreibung: 1-32

Beschreibung: 7T. Variazioni delle caratteristiche crostali e "precursori"

Beschreibung: 2TM. Divulgazione Scientifica

Beschreibung: N/A or not JCR

Beschreibung: La Cromatografia Ionica (IC) è diventata una delle tecniche analitiche più utilizzate nella determinazione degli anioni (F-, Cl-, Br-, NO3-, SO42-) e dei cationi (Li+, Na+, K+, Mg2+, Ca2+) in soluzioni acquose, anche a basse concentrazioni. Nel Laboratorio dell’INGV Sezione di Palermo questa tecnica viene utilizzata per determinare la composizione chimica di acque naturali (acque sotterranee, termali, meteoriche, marine), di fluidi fumarolici, campionati tramite il metodo delle ampolle di Giggenbach e delle specie acide emesse dai pennacchi vulcanici campionati con trappole alcaline. Ogni anno nel laboratorio vengono analizzati numerosi campioni per scopi di ricerca e monitoraggio geochimico e ambientale. Uno dei principali problemi dell’attività analitica di routine è la valutazione della precisione del dato, definita dall’American Public Health Association [1999], “come la misura del grado di accordo tra analisi replicate di un campione, di solito espresse come deviazione standard”. Tralasciando I problemi di rappresentatività del campione, ci sono diversi fattori che contribuiscono alla precisione, come il pretrattamento del campione, la preparazione e conservazione delle miscele di calibrazione, la ripetibilità delle misure delle aree dei picchi delle miscele di calibrazione e il tipo di calibrazione. In questo lavoro mostriamo la precisione dei cromatografi Dionex ICS-1100 ripetendo più volte l’analisi le miscele di calibrazione durante una singola sessione analitica, e la ripetibilità dello strumento, elaborando I risultati ottenuti analizzando le stesse miscele di calibrazione in differenti sessioni analitiche nell’arco di 12 mesi. Infine, viene determinata l’accuratezza del metodo attraverso l’analisi di un campione certificato. L’accuratezza è la vicinanza tra il valore osservato ed il valore vero, o comunque accettato come tale. I dati riportati in questo rapporto tecnico si riferiscono alle condizioni analitiche per le analisi di routine, con la configurazione strumentale corrente in uso presso questo laboratorio; queste possono essere ottimizzate per altri range di utilizzo a condizione però che vengano eseguite le necessarie verifiche di precisione e accuratezza.

Beschreibung: Istituto Nazionale di Geofisica e Vulcanologia (INGV) “Sez. di Palermo”

Beschreibung: Published

Beschreibung: 1-22

Beschreibung: 2IT. Laboratori analitici e sperimentali

Beschreibung: N/A or not JCR

Beschreibung: A new indirect method of estimating in situ soil air permeability is presented in this technical report. The new method is simply based on the measurement of air pressure (probe pressure) generated by pumping a constant air flux inside a special designed probe inserted into the soil. To calibrate the method, some measurements of the probe pressure were performed in some soils of the island of Vulcano, characterized by different values of the air permeability. Furthermore, technical aspects of a new device for performing continuous acquisition of the air permeability based on the new indirect method, were also described

Beschreibung: Istituto Nazionale di Geofisica e Vulcanologia (INGV) “Sez. di Palermo”

Beschreibung: Published

Beschreibung: 1-18

Beschreibung: 1IT. Reti di monitoraggio e sorveglianza

Beschreibung: N/A or not JCR

Beschreibung: La determinazione di anioni inorganici nelle acque naturali, sotterranee e superficiali, cosi come nelle acque reflue e potabili, è una delle applicazioni più importanti della cromatografia ionica (IC). Con questa tecnica si possono comunemente determinare i principali anioni inorganici: fluoruro, cloruro, bromuro, nitrato e solfato. Utilizzando specifiche metodologie oltre ai predetti analiti, si può determinare anche il fosfato durante la medesima analisi. La determinazione dei fosfati nelle acque riveste una notevole importanza in considerazione del fatto che il fosfato rappresenta un possibile indice di inquinamento per la presenza di questa sostanza in moltissimi composti chimici, quali: detersivi domestici ed industriali, fertilizzanti ecc. La quantità di fosfati, nelle acque naturali, quando è di origine minerale e non indica inquinamento, raggiunge concentrazioni appena dosabili, mentre concentrazioni elevate e quindi misurabili, sono indice di inquinamento. Il fosfato è uno dei nutrienti di maggiore importanza nelle acque, la sua presenza in concentrazioni consistenti, può essere responsabile di manifestazioni eutrofiche ed ipertrofiche nelle acque superficiali. Data la sua importanza nel condizionare il funzionamento degli ecosistemi acquatici, esso è incluso nella lista dei cosiddetti macrodescrittori utilizzati per definire lo stato chimico delle acque fluviali e lacustri e per valutare, lo stato trofico delle acque. La determinazione del fosfato viene comunemente effettuata utilizzando l’analisi spettrofotometrica dell’eteropoliacido fosfomolibdico formatosi in seguito alla reazione con molibdato in ambiente acido ed in presenza di idoneo riducente. Tuttavia questo, metodo ampliamente utilizzato, consente la determinazione del singolo analita e non consente quindi la caratterizzazione chimica del campione. La cromatografia ionica, invece, presenta il vantaggio di essere una tecnica multi-elementare che consente di determinare in breve tempo tutti i costituenti maggiori disciolti nelle acque. In questo rapporto tecnico viene descritta la determinazione del fosfato e dei costituenti maggiori nelle acque naturali utilizzando, un cromatografo Thermo Scientific Dionex ICS 5000+, equipaggiato con una colonna a scambio anionico “Dionex AS19 4µm”, un generatore di eluente a idrossido di potassio (KOH), un soppressore elettrochimico ed un rivelatore a cella conduttimetrica.

Beschreibung: Istituto Nazionale di Geofisica e Vulcanologia (INGV) “Sez. di Palermo”

Beschreibung: Published

Beschreibung: 1-22

Beschreibung: 2IT. Laboratori analitici e sperimentali

Beschreibung: N/A or not JCR

Beschreibung: Il sito delle “Ciampate del diavolo”, sul fianco nord­orientale del vulcano Roccamonfina, è un sito unico al Mondo grazie alle sue peculiarità geologiche ed antropologiche. Il ritrovamento di impronte di hominini al di sopra di un deposito ignimbritico, impresse in nel periodo di maggiore attività del vulcano, dà spazio a numerose questioni circa le condizioni paleoambientali esistenti sul vulcano ed i complessi processi che hanno permesso la formazione e la conservazione delle impronte. La nota dedica particolare attenzione al contesto dell’attività del vulcano ed alle caratteristiche della serie dei Tufi Leucitici Bruni, in quanto le impronte note come “Ciampate del diavolo” sono presenti sulla superficie di una delle unità più alte della sequenza di questi tufi. The “Ciampate del diavolo” geosite is unique in the World due to its geological and anthropological peculiarities. Finding hominin footprints on the ignimbrite deposits of 350,000 years ago, imprinted in the period of greatest activity of the volcano, opens up numerous questions about the palaeoenvironmental conditions of the volcano and the complex processes that allowed the generation and conservation of the footprints. The note pays particular attention to the framing of the volcano's activity and to the features of deposits of the Brown Leucitic Tuff series, as the human fossil footprints known as "Ciampate del Diavolo" are preserved on the surface of one of the highest units in the sequence.

Beschreibung: Published

Beschreibung: 49-55

Beschreibung: 1V. Storia eruttiva

Beschreibung: 5V. Processi eruttivi e post-eruttivi

Beschreibung: 1TR. Georisorse

Beschreibung: 6SR VULCANI – Servizi e ricerca per la società

Beschreibung: N/A or not JCR

Beschreibung: Mts. Simbruini karst aquifer feeds important springs whose capture contributes to the water supply of Rome City. To improve the geochemical characterization of this aquifer, we analyzed 36 groundwater samples, 29 from springs and 7 from shallow wells, collected in 1996 and 2019. Atomic adsorption spectroscopy, titration, ionic chromatography and mass spectrometry were the used analytical methods. Groundwater is bicarbonate alkaline-earth type and HCO3- dominance confirms that the aquifer is hosted in carbonate rocks. Total alkalinity vs. cations plot indicates that CO2 driven weathering controls the water chemistry. The probability plots of HCO3-, cations and Ca2+ +Mg2+ indicate four groundwater populations with the less represented one (9 samples)characterized by the highest PCO2 values (〉 0.3 atm). Most anomalous values of the dissolved PCO2are from springs located near the center of the studied area. Four samples have negative values of𝛿13CCO2 (about -22‰ vs. PDB), indicating its organic origin, but two other samples have positivevalues (1.6 and 2.6 ‰ vs. PDB), similar to those observed in the CO2 of deep origin discharged atthe close Colli Albani volcano. Therefore, geochemical evidence indicates that the Mts. Simbruiniaquifer is locally affected by the input of deep originated CO2, likely rising up along fractures, interacting with a recharge of meteoric origin, as evidenced by its 𝛿2H and 𝛿18O isotopic signatures.

Beschreibung: Published

Beschreibung: HS659

Beschreibung: 1TR. Georisorse

Beschreibung: JCR Journal

Beschreibung: The best chance to achieve a future disaster-resilient society is through risk education in School: it has a great potential to strengthen capacity of communities to mitigate risks. The KnowRISK (Know your city, Reduce seISmic risK through non-structural elements) project took this opportunity and implemented a risk communication campaign for schools in Portugal, Italy, and Iceland. The idea was that suitably changes in people’s knowledge and attitude can trigger best practices. Crucial to reach such target is the raise of awareness on meaningful issues. The main challenge of the campaign was how to effectively address the mitigation of the vulnerability to earthquakes of non-structural elements, which is an issue considered to be of low priority even in the building regulations of many countries around the world. The campaign stood on a communication strategy that was systematized within a protocol, for 13- 15 years old students, that specifies goals, contents, learning strategy, support material, and relies on face-to-face intervention of scientists in the classroom. This protocol had training sessions bounded by assessment sessions, ex-ante and ex-post, that allowed to validate its efficacy. The training made large use of flipped learning and Episode of Situated Learning (EAS) strategy to raise student’s motivation and increase achievements. To ensure its replicability, the protocol was tested in zones matching a wide range of seismic hazard in Italy. The assessment showed the protocol be effective and ready for a wide dissemination.

Beschreibung: Published

Beschreibung: SE325

Beschreibung: 2TM. Divulgazione Scientifica

Beschreibung: JCR Journal

Beschreibung: In aree di vulcanismo attivo e recente, oltre all’emissione di vapore e gas dai crateri centrali, si possono verificare emissioni di gas dal suolo che vengono rilasciati in modo diffuso o in mofete, o ancora che si disciolgono in acquiferi superficiali. Generalmente il gas più abbondante (fino al 99 vol.%) è l’anidride carbonica (CO2), ma in alcuni casi può essere anche il metano (CH4). La CO2 è un gas incolore e inodore che tende ad accumularsi in depressioni o scantinati dove ristagna in assenza di vento. Respirare aria con una concentrazione di anidride carbonica maggiore di 8 vol.% può condurre all’incoscienza o alla morte. Un esempio di quello che potrebbe accadere, anche se si tratta di un caso estremo, è rappresentato dal tragico evento avvenuto presso il lago Nyos in Camerun (un lago ospitato in un cratere vulcanico). Durante la notte del 21 agosto 1986 le acque del lago, sature di CO2, si rovesciarono improvvisamente e per decompressione si liberò una enorme quantità di gas che scese lungo i fianchi del cratere fino a raggiungere la valle sottostante dove vi era un villaggio. La nube di CO2, silenziosa e inodore, colse nel sonno gli abitanti e uccise circa 1700 persone e circa 3000 capi di bestiame [Barberi et al., 1986]. Numerosi incidenti dovuti all’inalazione di gas vulcanici sono avvenuti in varie altre parti del mondo, in particolare in Italia, Giappone, Nuova Zelanda [Hansell and Oppenheimer, 2004; Durand and Wilson, 2005] e nelle Isole Azzorre (Portogallo) [Viveiros et al., 2015]. Anche in Italia, purtroppo sono avvenuti diversi incidenti letali dovuti ad inalazione di CO2; si ricorda ad esempio che alla fine degli anni ’80 due bambini persero la vita nell’isola di Vulcano [Baubron et al., 1990] e ancora nel complesso vulcanico dei Colli Albani due uomini persero la vita, il primo a Cava dei Selci (frazione di Marino) nel 2000 e il secondo a Lavinio nel 2011 [Carapezza et al., 2003; Barberi et al., 2019]. Sempre in provincia di Roma, numerosi casi di intossicazione da CO2, che hanno altresì comportato l’evacuazione temporanea di alcune abitazioni, sono avvenuti per blowout (emissione incontrollata) di gas da pozzi d’acqua [Barberi et al., 2007; Carapezza et al., 2020]. La Campania ospita due dei vulcani quiescenti considerati tra i più pericolosi al mondo proprio per l’alta densità di popolazione che vive nelle zone esposte al pericolo: il Vesuvio e i Campi Flegrei. Anche in queste aree vulcaniche si hanno emissioni di gas endogeni e falde d’acqua ricche in CO2 e in caso di riattivazione del vulcano c’è da aspettarsi anche un forte incremento del rilascio del gas endogeno [Barberi et al., 2005]. Al fine di far conoscere tale problematica alla popolazione, si è ritenuto opportuno di agire sui ragazzi e di farlo in modo stimolante e divertente attraverso un Videogioco che catturi la loro attenzione in modo da portarli a scoprire le soluzioni più adeguate da adottare per individuare/evitare/gestire i pericoli legati a quello che spesso viene definito anche “carburante delle eruzioni”, i gas vulcanici. Le attività che hanno portato alla realizzazione di questo lavoro (e nello specifico del videogioco) sono state svolte nell’ambito del Progetto Europeo RESPIRE – Radon rEal time monitoring System and proactive Indoor Remediation (LIFE16ENV/IT/000553) e con la collaborazione di un Tirocinante del Dipartimento di Ingegneria dell’Informazione ed Elettrica e Matematica Applicata dell’Università degli Studi di Salerno. Il lavoro è stato descritto e sintetizzato in questo Report attraverso varie sezioni. La prima in cui si descrive la problematica dei Gas Vulcanici e della loro pericolosità; l’importanza e i vantaggi derivati dall’utilizzo di un videogioco come strumento di apprendimento; l’obiettivo che il videogioco si prefigge di raggiungere. Una seconda sezione in cui, in prima istanza, si evidenzia l’importanza di sviluppare un videogioco a partire da un Motore Grafico che consente di tralasciare i dettagli hardware e software di basso livello e di concentrarsi maggiormente sull’interattività e sulle regole del gioco, e in seconda istanza si descrivono le caratteristiche principali del motore grafico alla base del gioco (RPG Maker MV).Una terza sezione in cui viene presentato il videogioco sviluppato denominato “GioGas”; nello specifico, la sua trama, l’interfaccia grafica che lo caratterizza e alcuni sui dettagli implementativi. Infine, una sezione in cui vengono descritti gli sviluppi futuri come ad esempio la divulgazione presso le scuole e in occasione di eventi, l’implementazione di una versione multiplayer del gioco al fine di aggiungere ulteriori elementi di stimolo e di coinvolgimento per lo studente.

Beschreibung: Published

Beschreibung: 6SR VULCANI – Servizi e ricerca per la società

Beschreibung: N/A or not JCR

Beschreibung: PGS1 is a new compact portable seismic station, designed at INGV OBS and Earth Lab, that is specifically intended for the deployment of dense arrays of seismographs on-shore. With its low cost, compact design, high data-quality and long battery life, PGS1 is a perfect solution for seismic monitoring networks. PGS1 design is based on a solid polypropylene suitcase, containing a complete data acquisition system, two battery packs and a photovoltaic panel. The new Earth Lab 5s medium-period seismic sensor is included. The whole system meets the IP67 standard requirements both in transport and in acquisition configuration. PGS1 is normally equipped with one battery pack, one more pack can be added inside the suitcase achieving 40 days of battery life. The station is equipped with a photovoltaic panel, useful to extend the deployment length. Inside the suitcase, there are compartments where to store the seismic sensor, the photovoltaic panel and all the cables. Therefore, the station is very easy to transport.

Beschreibung: Published

Beschreibung: SE105

Beschreibung: 1IT. Reti di monitoraggio e sorveglianza

Beschreibung: JCR Journal

Beschreibung: Low field magnetic susceptibility and other rock magnetic analyses are applied to inspect the magnetic nature of solid residuals in snow samples collected in downtown Rome and in two Natural Parks in central Italy. Field emission scanning electron microscope and energy dispersive spectroscopy (FESEM-EDS) analyses are utilized to reveal the nature of Fe-rich particles and discriminate their anthropogenic origin. The results indicate that magnetite (Fe3O4) is the main magnetic carrier in almost all samples and that the variations in concentration are directly associated with local sources of particulate matter (PM) from anthropogenic pollution related to automotive circulation in both environments. Magnetic minerals of other provenance are found as accessories. The snow deposits revealed to be an efficient neutral tool for fine particle collections, also in environments characterized by different concentration and source of pollutants.

Beschreibung: Published

Beschreibung: GM215

Beschreibung: 1A. Geomagnetismo e Paleomagnetismo

Beschreibung: JCR Journal

Beschreibung: The 1971 eruption represents a benchmark in the recent history of Etna volcano. From a volcanological point of view, this eruption was characterised by complex intrusive dynamics associated with significant ground deformation that induced the activation of the Moscarello seismogenic fault and the formation of a new summit crater: the Southeast Crater. At the same time, the 1971 event marks an important change in the eruptive style and composition of the magma towards products richer in K. It is no coincidence that, over the next fifty years, there would be an increase in the frequency of summit and flank eruptions and associated output rate. From an historical viewpoint, the eruptive event of 1971 was the first important flank eruption studied by the International Institute of Volcanology: the analysis of the scientific articles on this activity reveals a greater multidisciplinary content in the descriptions and explanations of volcanic activity. Particularly important were the collaborations of British and French research groups that, together with their Italian colleagues, succeeded in giving a complete picture of the eruption and describing the state of knowledge on the Sicilian volcano. The multidisciplinary methodology used to study this eruption is still valid today.

Beschreibung: Published

Beschreibung: VO543

Beschreibung: 1V. Storia eruttiva

Beschreibung: JCR Journal

Beschreibung: This study analyses the relationship between the pre- and protohistoric sites on the slopes of Etna and the volcanic products, as well as the diverse settlement strategies in the different periods of prehistory. New C14 dating from significant excavations, in addition to those known from other Etnean sites, were performed with the aim of validating the chronology of the sequence of the different phases. A substantial concordance of the archaeological data with the volcanological ones has been found. It has been observed that a consistent human presence on Etna appears from the Middle Neolithic (5500 BC), after the sequence of eruptive events that marked the end of the Ellittico volcano (13550 - 13050 BC) and the formation of the Valle del Bove, and the subsequent debris and alluvial events on the eastern flanks of the volcano (7250 - 3350 BC). Human presence intensifies between the Late-Final Copper Age and the Early Bronze Age (2800 - 1450 BC), due to improvement in subsistence techniques and to the large presence of soils on lava flows suitable for sheep farming. The most recent phases of the Bronze Age are poorly represented, probably because of the concentration of the population in larger agglomerations (Montevergine and S. Paolillo at Catania, the Historical Hill at Paternò). The explosive eruptions taking place in this period seem to have had less impact on the settlement choices and have not affected the development of the sites over time.

Beschreibung: Published

Beschreibung: VO542

Beschreibung: 1V. Storia eruttiva

Beschreibung: JCR Journal

Beschreibung: This special issue of Annals of Geophysics entitled: “When Volcanologists Meet Archaeologists and Other Disciplines: Relationships Between Eruptions and Human Communities” originates from a session (S13) of the Rittmann International Conference which took place in Catania on February 13th 2020, having as its main theme the history of volcanology and the impact of volcanic activity on humans. The twelve articles collected in this special issue reflect the aims and contents of the reports presented by some participants at this session of the Rittmann conference

Beschreibung: Published

Beschreibung: 6SR VULCANI – Servizi e ricerca per la società

Beschreibung: JCR Journal

Beschreibung: Published

Beschreibung: 6TM. Poli Museali

Beschreibung: The Western Ionian Sea is characterised by an active and diffuse seismicity, directly related to the convergence of the European and African Plates and by gravitational sinking and rollback of the oceanic lithosphere. In this area, the location of earthquakes is characterised by considerable uncertainties due to large azimuthal gaps, resulting in notable location errors. This problem was partially overcome with the use of data recorded by NEMO-SN1 seafloor observatory (October 2002 - February 2003; June 2012 - May 2013). We relocated 1130 crustal and sub-crustal earthquakes using land network and NEMO-SN1 data. As most events occurred on Mt. Etna, we focused on 358 earthquakes in the offshore area and near the coasts of Sicily and Calabria. The use of the combined land-marine networks has improved the earthquake locations in terms of azimuthal GAP, as well as in horizontal and vertical errors. The comparison between locations performed with and without NEMO-SN1 data shows that differences in latitude, longitude and depths are more evident in the Western Ionian Sea and in the coast of Sicily, where values of the differences over 5 km correspond to structural heterogeneities. The increased number of seismic stations deployed on land from 2003 to 2012 did not influence the location of events occurring offshore, where NEMO-SN1 continued to be the distinctive tool in the location process. Moreover, the new 73 focal mechanisms computed with P-wave polarities from NEMO-SN1 and land stations are in agreement with the regional structural model, showing a prevalent normal, normal/oblique, and strike-slip kinematics. The similarity of two new focal solutions with the mechanisms of the main shock and aftershock of the 1990 earthquake demonstrates that the seismic structures are still active and potentially dangerous. The P-wave travel time residual analysis confirms the activity along the main structural alignments. A single point of observation in the Ionian Sea can significantly improve the quality of locations, giving an opportunity to focus on the seismogenic structures responsible for the occurrence of medium-to-high magnitude earthquakes.

Beschreibung: Published

Beschreibung: Se655

Beschreibung: 1T. Struttura della Terra

Beschreibung: JCR Journal

Beschreibung: The 8 September 1905 Calabria earthquake is the seismic event for which the Italian Seismic Catalogue shows the highest instrumental magnitude of the whole dataset. However, the reported MS = 7.47 was calculated over only two stations, and leaves room for a revision. In this work I provide a new estimate of the surface-wave magnitude of the earthquake calculated over sixteen individual values of magnitude from seven different stations. The new estimate is MS = 7.10 ± 0.21, a value that is consistently lined up with other estimates provided by means of macroseismic or geological evidence. The novel estimate is stable despite alternative epicentral locations and different depths proposed for this event by several investigators. The net variation of almost half a unit magnitude implies a resizing of the seismogenic source of the event in the frame of the seismotectonics of the region, and highlights the strong need for a systematic revision of the instrumental magnitude estimates for several ‘historical’ earthquakes that occurred at the dawning of the instrumental seismology.

Beschreibung: Published

Beschreibung: SE658

Beschreibung: 4T. Sismicità dell'Italia

Beschreibung: JCR Journal

Beschreibung: Usually seismicity is intended as a natural phenomenon; yet the recording and interpretation of data are a human undertaking, historically conditioned. This research starts from the hypothesis that the decrease in Italian seismicity observed in the historical catalog [Rovida et al., 2021] for the central years of the twentieth century is due to the social and political conditioning that hindered its registration. This work, which focuses on the 1930s, with a brief excursion into previous years, presents part of the results obtained. In particular, the article deals with three earthquakes of which the data are substantially updated (Calabria, 7.03.1928; Alpi Noriche, 14.05.1930; Valli Giudicarie, 14.04.1931); then it presents an important replica of the strong Maiella earthquake, without macroseismic data in the catalog [Rovida et al., 2021], (Maiella, 23.11.1933); finally it considers a “forgotten” fifth earthquake (Southern Italy, 13.04.1938).

Beschreibung: Published

Beschreibung: 1-78

Beschreibung: 4T. Sismicità dell'Italia

Beschreibung: JCR Journal

Beschreibung: Le finalità del progetto sono quelle di realizzare un prototipo di ionosonda per sondaggi obliqui basato sul principio del radar ad onda continua modulata in frequenza, implementato tramite dispositivi programmabili innovativi che permettono di realizzare uno strumento più piccolo e trasportabile rispetto alle ionosonde classiche. La ionosonda SDR è un radar bistatico costituito da un trasmettitore, che può servire contemporaneamente diversi ricevitori situati in località differenti, e da uno o più ricevitori che possono sintonizzarsi su differenti trasmettitori che effettuano sondaggi ad orari differenti. I sondaggi obliqui permettono di mappare la ionosfera in varie parti del territorio per la verifica di modelli teorici della ionosfera e per studiare la possibilità di usare le variazioni locali del Contenuto Elettronico Totale (TEC) come segnale di possibili precursori di terremoti.

Beschreibung: Published

Beschreibung: 1-32

Beschreibung: 6IT. Osservatori non satellitari

Beschreibung: N/A or not JCR

Beschreibung: The Istituto Nazionale di Geofisica e Vulcanologia (INGV) was born from the unification of public research institutions in the field of geophysics and volcanology. In the first twenty years from the foundation law of 1999, the INGV has seen a remarkable development, alternating moments of expansion, with significant increases in funding and number of employees, with long periods of stagnation. During these years the number of people hired with fixed-term contracts has grown, also to meet the needs of seismic and volcanic monitoring of the Italian territory, carried out on the basis of agreements with the Department of Civil Protection. After twenty years, it's time to outline a picture of the present situation and the evolutionary process that determined it. With this objective, the following analysis of the evolution of INGV was carried out in the period between its establishment and 2019, with reference to aspects such as the composition of the personnel, its distribution in the Sections, age, gender, role, career, with the aim of providing a tool for evaluating the progress made and planning future developments. The presence of a large number of precarious workers, distributed unevenly in territorial offices, for many years represented the main problem of the INGV, but not the only one. The contraction of the funding and the hiring freeze also slowed the development of the careers of researchers and other employers. The poor gender balance among the personnel, in particular among researchers, and the high average age represents further critical elements. The appropriate rebalancing of heterogeneities, aimed at maximum functionality and effective growth of the institute, should be the challenge for the next years. The future of INGV will depend on the choices that will be made today and the knowledge of the evolution of the institution until today is an essential element for proper programming.

Beschreibung: Published

Beschreibung: 1-92

Beschreibung: 3TM. Comunicazione

Beschreibung: JCR Journal

Beschreibung: Istituto Nazionale di Geofisica e Vulcanologia

Beschreibung: Published

Beschreibung: 1-186

Beschreibung: 1SR TERREMOTI - Sorveglianza Sismica e Allerta Tsunami

Beschreibung: 1IT. Reti di monitoraggio e sorveglianza

Beschreibung: 1TM. Formazione

Beschreibung: 2TM. Divulgazione Scientifica

Beschreibung: N/A or not JCR

Beschreibung: This contribution illustrates the state of the art on the development of the multiparametric network in Sicily in the last three years. This region is wellknown for the strong earthquakes (M〉6.5) which struck in historic time. However, the coverage of the seismic network developed in the last decades was not fully optimized to the need of the seismic surveillance. Since 2017, the group belonging to the National Earthquake Observatory (ONT) which operates in Sicily launched a developing plan of the preexisting seismic network in the framework of the project FISR “Sale Operative Integrate e Reti di Monitoraggio del futuro: l’INGV 2.0 (S.O.I.R.)”. Such update will end by the next years thanks to the project “GRINT Italian Research Infrastructure for Geosciences” in the framework of the “Programma Operativo Nazionale” (PON) of the Italian Ministry for the University and Research. This plan envisages both the upgrade of monitoring systems in the already existing nodes, and the integration of new nodes. The main objective is to build a multiparametric network which integrates the main seismic network, with the monitoring of other geophysical signals through the implementation of other sensors: accelerometers, geodetic GPS, radon detectors, and corner reflectors. We also illustrate the plan for the realization of a redundant network which could support the tasks of the main infrastructure in case of failure of the latter. Finally, we present some experimental urbanscale networks which in the future could support the main infrastructure.

Beschreibung: Published

Beschreibung: 1-40

Beschreibung: 1IT. Reti di monitoraggio e sorveglianza

Beschreibung: JCR Journal

Beschreibung: This article has been accepted for publication in Geophysical Journal International ©: The Authors 2021. Published by Oxford University Press on behalf of the Royal Astronomical Society. All rights reserved. Uploaded in accordance with the publisher's self-archiving policy.

Beschreibung: Ambient-noise records from the AlpArray network are used to measure Rayleigh wave phase velocities between more than 150,000 station pairs. From these, azimuthally anisotropic phase-velocity maps are obtained by applying the Eikonal tomography method. Several synthetic tests are shown to study the bias in the Ψ2 anisotropy. There are two main groups of bias, the first one caused by interference between refracted/reflected waves and the appearance of secondary wavefronts that affect the phase travel-time measurements. This bias can be reduced if the amplitude field can be estimated correctly. Another source of error is related to the incomplete reconstruction of the travel-time field that is only sparsely sampled due to the receiver locations. Both types of bias scale with the magnitude of the velocity heterogeneities. Most affected by the spurious Ψ2 anisotropy are areas inside and at the border of low-velocity zones. In the isotropic velocity distribution, most of the bias cancels out if the azimuthal coverage is good. Despite the lack of resolution in many parts of the surveyed area, we identify a number of anisotropic structures that are robust: in the central Alps, we find a layered anisotropic structure, arc-parallel at midcrustal depths and arc-perpendicular in the lower crust. In contrast, in the eastern Alps, the pattern is more consistently E-W oriented which we relate to the eastward extrusion. The northern Alpine forleand exhibits a preferential anisotropic orientation that is similar to SKS observations in the lowermost crust and uppermost mantle.

Beschreibung: German Science Foundation (SPP-2017, Project Ha 2403/21-1); Swiss National Science Foundation SINERGIA Project CRSII2-154434/1 (Swiss-AlpArray); Progetto Pianeta Dinamico, finanziamento MUR-INGV, Task S2 – 2021

Beschreibung: Published

Beschreibung: 151–170

Beschreibung: 1T. Struttura della Terra

Beschreibung: JCR Journal

Beschreibung: The present paper describes the process of moving from a research study of most common vulnerable non-structural elements, to deliver solutions, tools and guidelines to improve understanding of and responsiveness to community concerns about seismic risk and non-structural elements. The observed damage to non-structural elements following recent earthquakes in Italy, Portugal and Iceland, were used for designing communication tools under the KnowRISK EU project for multi-stakeholders (students, business and citizens): the Practical Guide, the Students Short Guide, the KnowRISK Portfolio of Solutions, the Move, Protect and Secure video, the augmented reality apps, the maquettes, the students notebooks, videos, board games and hands-on tools. The philosophy behind these deliverables is that some risks, once identified, can be eliminated or reduced by informing people and suggesting preventive or emergency measures. These tools are devoted to improving the seismic performance of non-structural elements and to reduce the associated economic losses, loss of functionality, and potential threats to life safety. The rationale behind the selection of the information that people need to know for converting knowledge to more safety is discussed and a description of the transference of the findings of research to communication solutions is presented. The tools were planned following the engagement-model in risk communication to ensure that needs of communities and selected stakeholders were acknowledged, and that recipients are addressed in a way that appeals to them. Different media and communication channels such as print, television, online, face-to face communication and interviews were used for risk communication.

Beschreibung: This study was co-financed by the European Commission’s Humanitarian Aid and Civil Protection (Grant agreement ECHO/SUB/2015/718655/PREV28).

Beschreibung: Published

Beschreibung: SE322

Beschreibung: 5T. Sismologia, geofisica e geologia per l'ingegneria sismica

Beschreibung: JCR Journal

Beschreibung: This article has been accepted for publication in Geophysical Journal International ©: The Authors 2022. Published by Oxford University Press on behalf of the Royal Astronomical Society. All rights reserved. Uploaded in accordance with the publisher's self-archiving policy.

Beschreibung: To understand the seismotectonics and the seismic hazard of the study sector of the Northern Apennines (Italy), one of the most important earthquakes of magnitude Mw = 6.5 which struck the Lunigiana and Garfagnana areas (Tuscany) on 7 September 1920 should be studied. Given the early instrumental epoch of the event, neither geometric and kinematic information on the fault-source nor its fault-plane solution were available. Both areas were candidates for hosting the source fault and there was uncertainty between a normal fault with Apenninic direction or an anti-Apenninic strike-slip. We retrieved 11 focal parameters (including the fault-plane solution) of the 1920 earthquake. Only macroseismic intensity information (from 499 inhabited centres) through the KF-NGA inversion technique was used. This technique uses a Kinematic model of the earthquake source and speeds up the calculation by a Genetic Algorithm with Niching. The result is a pure dip-slip focal solution. The intrinsic ambiguities of the KF-NGA method (±180° on the rake angle; choice of the fault plane between the two nodal planes) were solved with field and seismotectonic evidence. The earthquake was generated by a normal fault (rake angle = 265° ± 8°) with an Apennine direction (114° ± 5°) and dipping 38° ± 6° towards SW. The likely candidate for hosting the source-fault in 1920 is the Compione-Comano fault that borders the NE edge of the Lunigiana graben. The KF-NGA algorithm proved to be invaluable for studying the kinematics of early instrumental earthquakes and allowed us to uniquely individuate, for the first time ever, the seismogenic source of the 1920 earthquake. Our findings have implications in hazard computation and seismotectonic contexts.

Beschreibung: Published

Beschreibung: 1465–1477

Beschreibung: 4T. Sismicità dell'Italia

Beschreibung: JCR Journal

Beschreibung: The lava platform and the three pyroclastic cones of Vulcanello constitute the northernmostvolcanic structure of the island of Vulcano (Aeolian Islands). The sandy isthmus connecting theplatform to the main island was definitively formed in the first half of the 1500s; before then,Vulcano and Vulcanello were two close but separate islands. For a long time, the interpretation ofthe sources of the II-I century BC, had considered the islet as built up about 2200 years ago. Thisbelief, which proliferated among naturalists from the 17th century, is not confirmed in the ancienttexts or even in the geographical documents of the time, which do not indicate the presence ofVulcanello as a new and stable island near Vulcano. The islet would only be mentioned at the dawnof the second millennium, and named in Arabic “Gabal’ al Burkān”, meaning Mount of Vulcano;shortly thereafter the toponym changed to the Latin “Insulam Vulcanelli” and then, towards the15th century, finally to Vulcanello.Since the creation of a volcanic island certainly occurred in the Aeolian Islands in the classical era,but traces of it were quickly lost, the most plausible hypothesis is that it was formed in the area ofthe current Vulcanello, to be subsequently erased by the sea. The shallow, flat seabed, likelyremaining as a result of sea abrasion, might have represented the morphological element on whichthe circular lava platform we know today was formed sometime between 950 and 1000 AD.

Beschreibung: Published

Beschreibung: VO548

Beschreibung: 1V. Storia eruttiva

Beschreibung: JCR Journal

Beschreibung: On November 20, 2019, an exercise was held at the National Institute of Geophysics and Volcanology (INGV) as part of the activities planned for preparing for seismic emergencies. The training was "tabletop" with the simulation of an earthquake with a magnitude greater than the threshold required for the activation of the intervention procedures, described in the "Protocol of the Authority for the management of seismic and tsunami emergencies and Establishment of the Crisis Unit”, the flow of actions that follow was verified. The exercise involved the entire INGV even if the Rome headquarters was the most involved, and it was the second of this type after that carried out in 2015 [Pondrelli et al., 2016]. Main motivation of this training was the analysis of the efficiency of the Organization Protocol, a document that for the first time at INGV codifies the actions of the Crisis Unit and of the Institute in general on the occurrence of seismic events and / or seismic sequences and / or tsunamis. The Protocol has been formalized on the basis of the numerous experiences that the Institute has supported over many years, to honour its vocation in the monitoring and seismic surveillance of the national territory [Margheriti et al., 2021].

Beschreibung: Published

Beschreibung: 1-50

Beschreibung: 2SR TERREMOTI - Gestione delle emergenze sismiche e da maremoto

Beschreibung: N/A or not JCR

Beschreibung: Systematic variations in the crystal cargo and whole-rock isotopic compositions of mantle-derived basalts in the intraplate Dunedin Volcano (New Zealand) indicate the influence of a complex mantle-to-crust polybaric plumbing system. Basaltic rocks define a compositional spectrum from low-alkali basalts through mid-alkali basalts to high-alkali basalts. High-alkali basalts display clinopyroxene crystals with sector (hourglass) and oscillatory zoning (Mg#61–82) as well as Fe-rich green cores (Mg#43–69), whereas low-alkali basalts are characterized by clinopyroxenes with unzoned overgrowths (Mg#69–83) on resorbed mafic cores (Mg#78–88), coexisting with reversely zoned plagioclase crystals (An43–68 to An60–84 from core to rim). Complex magma dynamics are indicated by distinctive compositional variations in clinopyroxene phenocrysts, with Cr-rich zones (Mg#74–87) indicating continuous recharge by more mafic magmas. Crystallization of olivine, clinopyroxene and titanomagnetite occurred within a polybaric plumbing system extending from upper mantle to mid-crustal depths (485–1059 MPa and 1147–1286°C), whereas crystallization of plagioclase with subordinate clinopyroxene and titanomagnetite proceeded towards shallower crustal levels. The compositions of high-alkali basalts and mid-alkali basalts resemble those of ocean island basalts and are characterized by FOZO-HIMU isotopic signatures (87Sr/86Sri = 0.70277–0.70315, 143Nd/144Ndi = 0.51286–0.51294 and 206Pb/204Pb = 19.348–20.265), whereas low-alkali basalts have lower incompatible element abundances and isotopic compositions trending towards EMII (87Sr/86Sri = 0.70327–70397, 143Nd/144Ndi = 0.51282–0.51286 and 206Pb/204Pb = 19.278–19.793). High- and mid-alkali basalt magmas mostly crystallized in the lower crust, whereas low-alkali basalt magma recorded deeper upper mantle clinopyroxene crystallization before eruption. The variable alkaline character and isotope composition may result from interaction of low-alkaline melts derived from the asthenosphere with melts derived from lithospheric mantle, possibly initiated by asthenospheric melt percolation. The transition to more alkaline compositions was induced by variable degrees of melting of metasomatic lithologies in the lithospheric mantle, leading to eruption of predominantly small-volume, high-alkali magmas at the periphery of the volcano. Moreover, the lithosphere imposed a filtering effect on the alkalinity of these intraplate magmas. As a consequence, the eruption of low-alkali basalts with greater asthenospheric input was concentrated at the centre of the volcano, where the plumbing system was more developed.

Beschreibung: Published

Beschreibung: egab062

Beschreibung: 2V. Struttura e sistema di alimentazione dei vulcani

Beschreibung: 3V. Proprietà chimico-fisiche dei magmi e dei prodotti vulcanici

Beschreibung: 4V. Processi pre-eruttivi

Beschreibung: JCR Journal

Beschreibung: Il mondo della Ricerca e dell ’Innovazione Tecnologica per sua natura è sempre in evoluzione, ed accompagnato da normative in materia di Sicurezza e Salute non sempre conosciute o applicate. La particolare enfasi data alla formazione nel quadro normativo riguardante la Sicurezza e Salute nei luoghi di lavoro, sia a livello nazionale che comunitario, dimostra e determina l’importanza attribuita a tale processo, non solo come mezzo elettivo per la diffusione della cultura della Sicurezza a tutti i livelli , ma anche, e soprattutto, come misura generale di tutela.

Beschreibung: Istituto Nazionale di Geofisica e Vulcanologia

Beschreibung: Published

Beschreibung: 1-54

Beschreibung: 1TM. Formazione

Beschreibung: N/A or not JCR

Beschreibung: Within a fault governing model the characteristic scale length is one of the most relevant physical parameters because it accounts for the so–called fracture energy (density) of the system, its dynamics, the time during which the accumulated stress is released and the seismic waves are excited, the amount of slip developed during an instability event. Friction laboratory experiments reveal that it is not a material property, but that it changes with the sliding velocity. We propose two rather different analytical models to fit laboratory evidence and we incorporate them into a fault model able to simulate repeated earthquakes in the framework of various formulations of rate and state friction. We demonstrate that temporal variations of the scale length do not prevent the system to reach its limit cycle, but they systematically reduce the magnitude of the expected event (both in term of developed slip, and thus seismic moment, and released stress) and also reduce the inter–event time (recurrence interval). Depending on the friction model, the system can penetrate into the stable regime and can either continue the accelerating phase toward to failure or decelerate and abort instability.

Beschreibung: Published

Beschreibung: SE217

Beschreibung: 3T. Fisica dei terremoti e Sorgente Sismica

Beschreibung: JCR Journal

Beschreibung: The understanding of the ancient Earth’s Magnetic Field strength, or absolute paleointensity, is fundamental for several disciplines of Earth Sciences. Following the growing interest in this field in the last decades, the number of paleointensity studies has been increasing and the Paleomagnetic Laboratory of the Istituto Nazionale di Geofisica e Vulcanologia (INGV) in Rome, Italy expanded the instrument park with a ASC TD48SC oven for paleomagnetic and paleointensity measurements. This manual represents an updated version of the original manual “Guida all’uso del forno ASC TD48SC per la stima della paleointensità assoluta con il metodo ThellierThellier modificato” [Di Chiara, 2014]. The aim is to provide a practical guide for paleointensity measurements, using the IZZIThellier experiment and the software Thellier_GUI for the processing of the geomagnetic field strength or paleointensity data.

Beschreibung: INGV

Beschreibung: Published

Beschreibung: 1-20

Beschreibung: 1A. Geomagnetismo e Paleomagnetismo

Beschreibung: N/A or not JCR

Beschreibung: We describe the geochemical characteristics of groundwater samples collected in 23 water wells located on the northern margin of the Hyblean plateau (East Sicily). This area, mostly made of highly permeable carbonate rocks, is rich in low temperature (T 〈 50° C) hydrothermal groundwaters, distributed in an active sismogenetic zone, with several ENE-WSW-directed tectonic structures that drove magma to the surface during Upper Pliocene and Pleistocene. The chemical features suggest complex mixing between rainwater, CO2-rich groundwater, steam-heated groundwater and geothermal brines, as highlighted by Principal Component Analysis (PCA). Some parameters, however, indicate widespread pollution of the aquifers from human activities. Stable isotopes analysis confirms the meteoric origin of groundwater and supports the origin of dissolved CO2 mostly from mantle degassing through deep tectonic faults. Geothermometric estimates, mostly based on quartz and Saturation Indexes geothermometers, suggest minimum reservoir temperature between 100 and 120° C.

Beschreibung: Published

Beschreibung: HS438

Beschreibung: 1TR. Georisorse

Beschreibung: JCR Journal

Beschreibung: A ground magnetic study was performed on the northern upper flank of Mt. Etna to provide new insights into subsurface volcano-tectonic structures. The high resolution magnetic survey was focused on the main structures of Piano delle Concazze, a large flat area dominated by the North- East crater and bounded by the rim of the Valle del Leone depression and the extremity of the North-East Rift. More than 2,500 measurements were gathered with a sampling step of about 3 m covering an area of about 0.2 km2. The total-intensity anomaly field shows the presence of intense South-North aligned maxima related to shallow geological structures affecting this area. Filtering techniques and 2.5D modeling have been applied for the determination of the magnetic source parameters. In order to distinguish the near surface structure, filters of the vertical derivatives, Butterworth high-pass and the tilt derivative were used. The 3D Euler deconvolution has been applied to estimate the depth and the structural indices of the causative sources. The calculated structural indices, that express the geometrical nature of the source, are in agreement with forward modeling. They show that the area is mainly affected by subvertical normal fault and the estimated depth of magnetic sources ranges between 10 m and 40 m. Our total field magnetic survey shows that characteristic magnetic anomalies are related to fault zones in the Piano delle Concazze that are well consistent with the local tectonics. The subsurface structures that have been detected allowed to delineate the general structural framework of the area. In particular, it was possible to clarify that these structures seem to be not deep rooted and consequently they can hardly act as preferential pathways for magma ascent.

Beschreibung: Published

Beschreibung: PE108

Beschreibung: 2V. Struttura e sistema di alimentazione dei vulcani

Beschreibung: JCR Journal

Beschreibung: The traction evolution is a fundamental ingredient to model the dynamics of an earthquake rupture which ultimately controls, during the coseismic phase, the energy release, the stress redistribution and the consequent excitation of seismic waves. In the present paper we explore the use of the friction behavior derived from laboratory shear experiments performed on granular materials at low normal stress. We find that the rheological properties emerging from these laboratory experiments can not be described in terms of preexisting governing models already presented in literature; our results indicate that neither rate–and state–dependent friction laws nor nonlinear slip–dependent models, commonly adopted for modeling earthquake ruptures, are able to capture all the features of the experimental data. Then, by exploiting a novel numerical approach, we directly incorporate the laboratory data into a code to simulate the fully dynamic propagation of a 3–D slip failure. We demonstrate that the rheology of the granular material, imposed as fault boundary condition, is dynamically consistent. Indeed, it is able to reproduce the basic features of a crustal earthquake, spontaneously accelerating up to some terminal rupture speed, both sub– and supershear.

Beschreibung: Published

Beschreibung: SE441

Beschreibung: 3T. Fisica dei terremoti e Sorgente Sismica

Beschreibung: JCR Journal

Beschreibung: INGV

Beschreibung: Published

Beschreibung: 1-18

Beschreibung: 1A. Geomagnetismo e Paleomagnetismo

Beschreibung: N/A or not JCR

Beschreibung: In the last decade, bottom-up strategies of risk education have raised importance, making seriousgames to become an alternative or complementary teaching tool for enhancing skills for acollaborative and adaptive response to social-ecological challenges. This study describes issues and challenges of serious games implemented within the framework oftwo European projects, namely UPStrat-MAFA (Urban Disaster Prevention Strategies usingMacroseismic Fields and FAult sources) and KnowRISK (Know your city, Reduce seISmic risKthrough non-structural elements); the goal is to instil in young people a proactive attitude towardsthe mitigation of seismic risk. The games were tested in some dissemination events focussed on fostering seismic riskpreparedness in students and improving good practices. We discuss the performance of our gameseven against more standard approaches to risk education. Our experience shows a rise of students’engagement compared to standard learning activities. The games were effective as students wereable to grasp the most relevant actions to reduce risk

Beschreibung: Published

Beschreibung: SE327

Beschreibung: 2TM. Divulgazione Scientifica

Beschreibung: JCR Journal

Beschreibung: A first combined absolute gravity and GNSS network of 5 stations distributed between Lazio, Umbria and Abruzzo regions, was realized in 2018 in order to lay the basics for a multidisciplinary approach to natural risk assessment in the area of Central Italy, affected by the 2009 and 2016 seismic activity. Up to now, two absolute gravity campaigns were carried out using the transportable Microg LaCoste FG5#238 and the portable A10#39 absolute gravimeters. The locations of gravimetric sites have been chosen indoor to allow optimal condition of measure; therefore, the heights of the indoor sites have been determined by joining the outdoor GNSS with classical topographic surveys. The good results obtained after the campaigns and data processing lay the foundations for a new multidisciplinary approach to study also seismogenetic areas. In this paper, we present the gravity and GNSS station monographs, together with the absolute gravity values and the coordinates resulting from the first field surveys.

Beschreibung: Published

Beschreibung: 1-26

Beschreibung: 4T. Sismicità dell'Italia

Beschreibung: JCR Journal

Beschreibung: Volcanic activity resumed during early Middle Ages times at Lipari following at least 6000 years of quiescence. This phenomenon occurred in a social context that had continuously developed from prehistoric times to the Roman age and was burdened by a demographic crisis that involved the archipelago between the 6th and 11th century AD. The rare archaeological records relating to the 6th - 11th centuries suggest abrupt changes in the population of the islands. The medieval sources are rich in religious and fantastic references to volcanic events linked to Lipari and Vulcano, testifying the uneasy condition for the human communities. This work concerns the resilience and adaptation of the communities to volcanic activity during the Late Middle Ages in Lipari. Starting from 1083 the Aeolian archipelago was involved in a repopulation program, implemented in 1095 by the Constitutum and organized by the Benedictine Monastery with the annexed S. Bartolomeo Cathedral on the castle. From the 13th century the volcanic phenomena, strictly limited to the northern sector of the island, did not interfere as previously with the anthropic activities. The Monastery will be enlarged in the Norman phase during the first half of the 12th century with the construction of the cloister. New historical documents relating to the 1264, report news of fires and land movements on Lipari. Recent age determinations obtained for the obsidian flow of Rocche Rosse at 1220 ± 30 AD (archaeomagnetic dating) and for an obsidian block of the Lami pyroclastic cone at 1243 ± 190 (fission-track dating) allow to define the age of the last phase of activity of the Monte Pilato-Lami-Rocche Rosse complex, and to associate it the events reported on 1264’s historical documents. This work makes in comparison volcanological, archaeological and historical dates and described an updated summary of one of the lesser known phases of the history of the archipelago. The main consequence of the medieval volcanic activity at Lipari caused a clear division of the territory with the population confined in the southeast quadrant, protected to the north by Serra and Monte Rosa which represented a natural orographic barrier.

Beschreibung: Published

Beschreibung: VO549

Beschreibung: 1V. Storia eruttiva

Beschreibung: JCR Journal

Beschreibung: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Page, H. N., Bahr, K. D., Cyronak, T., Jewett, E. B., Johnson, M. D., & McCoy, S. J. Responses of benthic calcifying algae to ocean acidification differ between laboratory and field settings. Ices Journal of Marine Science, 79(1), (2022): 1–11, https://doi.org/10.1093/icesjms/fsab232.

Beschreibung: Accurately predicting the effects of ocean and coastal acidification on marine ecosystems requires understanding how responses scale from laboratory experiments to the natural world. Using benthic calcifying macroalgae as a model system, we performed a semi-quantitative synthesis to compare directional responses between laboratory experiments and field studies. Variability in ecological, spatial, and temporal scales across studies, and the disparity in the number of responses documented in laboratory and field settings, make direct comparisons difficult. Despite these differences, some responses, including community-level measurements, were consistent across laboratory and field studies. However, there were also mismatches in the directionality of many responses with more negative acidification impacts reported in laboratory experiments. Recommendations to improve our ability to scale responses include: (i) developing novel approaches to allow measurements of the same responses in laboratory and field settings, and (ii) researching understudied calcifying benthic macroalgal species and responses. Incorporating these guidelines into research programs will yield data more suitable for robust meta-analyses and will facilitate the development of ecosystem models that incorporate proper scaling of organismal responses to in situ acidification. This, in turn, will allow for more accurate predictions of future changes in ecosystem health and function in a rapidly changing natural climate.

Beschreibung: We would like to thank the Ocean Carbon and Biogeochemistry Program for organizing the fourth U.S. Ocean Acidification Principal Investigators meeting, which is where this synthesis was conceived. HNP was a postdoctoral research fellow at Mote Marine Laboratory. MDJ is a postdoctoral scholar at Woods Hole Oceanographic Institution. SJM is a Norma J. Lang early career fellow of the Phycological Society of America.

Beschreibung: In this paper we simulate the earthquake that hit the city of L'Aquila on the 6th of April 2009 using SPEED (SPectral Elements in Elastodynamics with Discontinuous Galerkin), an open-source code able to simulate the propagation of seismic waves in complex three-dimensional (3D) domains. Our model includes an accurate 3D recon- struction of the Quaternary deposits, according to the most up-to-date data obtained from the Microzonation studies in Central Italy and a detailed model of the topography incorporated using a newly developed tool (May et al. 2021). The sensitivity of our results with respect to dfferent kinematic seismic sources is inves- tigated. The results obtained are in good agreement with the recordings at the available seismic stations at epicentral distances within a range of 20km. Finally, a blind source prediction scenario application shows a reasonably good agreement between simulations and recordings can be obtained by simulating stochastic rupture realizations with basic input data. These results, although limited to nine simulated scenarios, demonstrate that it is possible to obtain a satisfactory reconstruction of a ground shaking scenario employing a stochastic source constrained on a limited amount of ex-ante information. A similar approach can be used to model future and past earthquakes for which little or no information is typically available, with potential relevant implications for seismic risk assessment.

Beschreibung: Published

Beschreibung: 29–49

Beschreibung: 3T. Fisica dei terremoti e Sorgente Sismica

Beschreibung: JCR Journal

Beschreibung: For the phase refraction index of high frequency (HF) waves in the ionospheric medium exists a well-established theory. However, under the Quasi-Longitudinal (QL) conditions, scientific literature presents various formulas that are not equivalent and that, in some cases, give rise to wrong results. In the present study, further consequences of Booker's rule are discussed, illustrating the validity ranges of the above-mentioned approximate formulas; and the different regimes for applying such QL formulas are described, along with the consequences in simulating the ionospheric HF ray-tracing, oblique and vertical sounding, and absorption.

Beschreibung: Published

Beschreibung: PA003

Beschreibung: 2A. Fisica dell'alta atmosfera

Beschreibung: 7SR AMBIENTE – Servizi e ricerca per la società

Beschreibung: 3IT. Calcolo scientifico

Beschreibung: 7TM.Sviluppo e Trasferimento Tecnologico

Beschreibung: JCR Journal

Beschreibung: This article has been accepted for publication in Geophysical Journal International ©: The Authors 2022. Published by Oxford University Press on behalf of the Royal Astronomical Society. All rights reserved. Uploaded in accordance with the publisher's self-archiving policy.

Beschreibung: On 29 December 2020, a shallow earthquake of magnitude Mw 6.4 struck northern Croatia, near the town of Petrinja, more than 24 hours after a strong foreshock (Ml 5). We formed a reconnaissance team of European geologists and engineers, from Croatia, Slovenia, France, Italy and Greece, rapidly deployed in the field to map the evidence of coseismic environmental effects. In the epicentral area, we recognized surface deformation, such as tectonic breaks along the earthquake source at the surface, liquefaction features (scattered in the fluvial plains of Kupa, Glina and Sava rivers), and slope failures, both caused by strong motion. Thanks to this concerted, collective and meticulous work, we were able to document and map a clear and unambiguous coseismic surface rupture associated with the main shock. The surface rupture appears discontinuous, consisting of multi-kilometer en échelon right stepping sections, along a NW-SE striking fault that we call the Petrinja-Pokupsko Fault (PPKF). The observed deformation features, in terms of kinematics and trace alignments, are consistent with slip on a right lateral fault, in agreement with the focal solution of the main shock. We found mole tracks, displacement on faults affecting natural features (e. g. drainage channels), scarplets, and more frequently breaks of anthropogenic markers (roads, fences). The surface rupture is observed over a length of ∼13 km from end-to-end, with a maximum displacement of 38 cm, and an average displacement of ∼10 cm. Moreover, the liquefaction extends over an area of nearly 600 km² around the epicenter. Typology of liquefaction features include sand blows, lateral spreading phenomenon along the road and river embankments, as well as sand ejecta of different grain size and matrix. Development of large and long fissures along the fluvial landforms, current or ancient, with massive ejections of sediments is pervasive. These features are sometimes accompanied by small horizontal displacements. Finally, the environmental effects of the earthquake appear to be reasonably consistent with the usual scaling relationships, in particular the surface faulting. This rupture of the ground occurred on or near traces of a fault that shows clear evidence of Quaternary activity. Further and detailed studies will be carried out to characterize this source and related faults in terms of future large earthquakes potential, for their integration into seismic hazard models.

Beschreibung: Published

Beschreibung: 1394–1418

Beschreibung: 2T. Deformazione crostale attiva

Beschreibung: JCR Journal

Beschreibung: In the last years the scientific literature has been enriched with new models of the Moho depth in the Antarctica Continent derived by the seismic reflection technique and refraction profiles, receiver functions and seismic surface waves, but also by gravimetric observations over the continent. In particular, the gravity satellite missions of the last two decades have provided data in this remote region of the Earth and have allowed the investigation of the crust properties. Meanwhile, other important contributions in this direction has been given by the fourth International Polar Year (IPY, 2007–2008) which started seismographic and geodetic networks of unprecedented duration and scale, including airborne gravimetry over largely unexplored Antarctic frontiers. In this study, a new model for the Antarctica Moho depths is proposed. This new estimation is based on no satellite gravity measures, thanks to the availability of the gravity database ANTGG2015, that collects gravity data from ground-base, airborne and shipborne campaigns. In this new estimate of the Moho depths the contribution of the gravity measures has been maximized reducing any correction of the gravity measures and avoiding constraints of the solution to seismological observations and to geological evidence. With this approach a pure gravimetric solution has been determined. The model obtained is pretty in agreement with other Moho models and thanks to the use of independent data it can be exploited also for cross-validating different Moho depths solutions.

Beschreibung: Published

Beschreibung: 1404–1420

Beschreibung: 1T. Struttura della Terra

Beschreibung: JCR Journal

Beschreibung: Seismic reflection and refraction data were collected in 2007 and 2012 to reveal the crustal fabric on a single long composite profile offshore Prydz Bay, East Antarctica. A P-wave velocity model provides insights on the crustal fabric, and a gravity-constrained density model is used to describe the crustal and mantle structure. The models show that a 230-km- wide continent–ocean transition separates stretched continental from oceanic crust along our profile. While the oceanic crust close to the continent–ocean boundary is just 3.5–5 km thick, its thickness increases northwards towards the Southern Kerguelen Plateau to 12 km. This change is accompanied by thickening of a lower crustal layer with high P-wave velocities of up to 7.5 km s–1, marking intrusive rocks emplaced beneath the mid-ocean ridge under increasing influence of the Kerguelen plume. Joint interpretations of our crustal model, seismic reflection data and magnetic data sets constrain the age and extent of oceanic crust in the research area. Oceanic crust is shown to continue to around 160 km farther south than has been interpreted in previous data, with profound implications for plate kinematic models of the region. Finally, by combining our findings with a regional magnetic data compilation and regional seismic reflection data we propose a larger extent of oceanic crust in the Enderby Basin than previously known.

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Roman-Vendrell, C., Medeiros, A. T., Sanderson, J. B., Jiang, H., Bartels, T., & Morgan, J. R. Effects of excess brain-derived human alpha-synuclein on synaptic vesicle trafficking. Frontiers in Neuroscience, 15, (2021): 639414, https://doi.org/10.3389./fnins.2021.639414

Beschreibung: α-Synuclein is a presynaptic protein that regulates synaptic vesicle trafficking under physiological conditions. However, in several neurodegenerative diseases, including Parkinson’s disease, dementia with Lewy bodies, and multiple system atrophy, α-synuclein accumulates throughout the neuron, including at synapses, leading to altered synaptic function, neurotoxicity, and motor, cognitive, and autonomic dysfunction. Neurons typically contain both monomeric and multimeric forms of α-synuclein, and it is generally accepted that disrupting the balance between them promotes aggregation and neurotoxicity. However, it remains unclear how distinct molecular species of α-synuclein affect synapses where α-synuclein is normally expressed. Using the lamprey reticulospinal synapse model, we previously showed that acute introduction of excess recombinant monomeric or dimeric α-synuclein impaired distinct stages of clathrin-mediated synaptic vesicle endocytosis, leading to a loss of synaptic vesicles. Here, we expand this knowledge by investigating the effects of native, physiological α-synuclein isolated from the brain of a neuropathologically normal human subject, which comprised predominantly helically folded multimeric α-synuclein with a minor component of monomeric α-synuclein. After acute introduction of excess brain-derived human α-synuclein, there was a moderate reduction in the synaptic vesicle cluster and an increase in the number of large, atypical vesicles called “cisternae.” In addition, brain-derived α-synuclein increased synaptic vesicle and cisternae sizes and induced atypical fusion/fission events at the active zone. In contrast to monomeric or dimeric α-synuclein, the brain-derived multimeric α-synuclein did not appear to alter clathrin-mediated synaptic vesicle endocytosis. Taken together, these data suggest that excess brain-derived human α-synuclein impairs intracellular vesicle trafficking and further corroborate the idea that different molecular species of α-synuclein produce distinct trafficking defects at synapses. These findings provide insights into the mechanisms by which excess α-synuclein contributes to synaptic deficits and disease phenotypes.

Beschreibung: This work was supported by the NIH (NINDS/NIA R01NS078165 and R01NS078165-S1 to JM; NINDS U54-NS110435, R01-NS109209, and R21-NS107950 to TB); research funds from the Marine Biological Laboratory (to JM); grants from the UK Dementia Research Institute (DRI), which receives its funding from DRI Ltd., the UK Medical Research Council and Alzheimer’s Society, and Alzheimer’s Research UK (to TB); the Michael J. Fox Foundation (Ken Griffin Imaging Award to TB); a Parkinson’s Disease Foundation Stanley Fahn Award (PF-JFA-1884 to TB); the Eisai Pharmaceutical postdoctoral program to TB; and the Chan Zuckerberg Collaborative Pairs Initiative (to TB).

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Molino, G. D., Defne, Z., Aretxabaleta, A. L., Ganju, N. K., & Carr, J. A. Quantifying slopes as a driver of forest to marsh conversion using geospatial techniques: application to Chesapeake Bay coastal-plain, United States. Frontiers in Environmental Science, 9, (2021): 616319, https://doi.org/10.3389/fenvs.2021.616319.

Beschreibung: Coastal salt marshes, which provide valuable ecosystem services such as flood mitigation and carbon sequestration, are threatened by rising sea level. In response, these ecosystems migrate landward, converting available upland into salt marsh. In the coastal-plain surrounding Chesapeake Bay, United States, conversion of coastal forest to salt marsh is well-documented and may offset salt marsh loss due to sea level rise, sediment deficits, and wave erosion. Land slope at the marsh-forest boundary is an important factor determining migration likelihood, however, the standard method of using field measurements to assess slope across the marsh-forest boundary is impractical on the scale of an estuary. Therefore, we developed a general slope quantification method that uses high resolution elevation data and a repurposed shoreline analysis tool to determine slope along the marsh-forest boundary for the entire Chesapeake Bay coastal-plain and find that less than 3% of transects have a slope value less than 1%; these low slope environments offer more favorable conditions for forest to marsh conversion. Then, we combine the bay-wide slope and elevation data with inundation modeling from Hurricane Isabel to determine likelihood of coastal forest conversion to salt marsh. This method can be applied to local and estuary-scale research to support management decisions regarding which upland forested areas are more critical to preserve as available space for marsh migration.

Beschreibung: Funding for this study was provided by the United States Geological Survey’s Coastal/Marine Hazards and Resources Program and Ecosystems Mission Area.

Beschreibung: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Stunz, E., Fetcher, N., Lavretsky, P., Mohl, J., Tang, J., & Moody, M. Landscape genomics provides evidence of ecotypic adaptation and a barrier to gene flow at treeline for the arctic foundation species Eriophorum vaginatum. Frontiers in Plant Science, 13, (2022): 860439, https://doi.org/10.3389/fpls.2022.860439.

Beschreibung: Global climate change has resulted in geographic range shifts of flora and fauna at a global scale. Extreme environments, like the Arctic, are seeing some of the most pronounced changes. This region covers 14% of the Earth’s land area, and while many arctic species are widespread, understanding ecotypic variation at the genomic level will be important for elucidating how range shifts will affect ecological processes. Tussock cottongrass (Eriophorum vaginatum L.) is a foundation species of the moist acidic tundra, whose potential decline due to competition from shrubs may affect ecosystem stability in the Arctic. We used double-digest Restriction Site-Associated DNA sequencing to identify genomic variation in 273 individuals of E. vaginatum from 17 sites along a latitudinal gradient in north central Alaska. These sites have been part of 30 + years of ecological research and are inclusive of a region that was part of the Beringian refugium. The data analyses included genomic population structure, demographic models, and genotype by environment association. Genome-wide SNP investigation revealed environmentally associated variation and population structure across the sampled range of E. vaginatum, including a genetic break between populations north and south of treeline. This structure is likely the result of subrefugial isolation, contemporary isolation by resistance, and adaptation. Forty-five candidate loci were identified with genotype-environment association (GEA) analyses, with most identified genes related to abiotic stress. Our results support a hypothesis of limited gene flow based on spatial and environmental factors for E. vaginatum, which in combination with life history traits could limit range expansion of southern ecotypes northward as the tundra warms. This has implications for lower competitive attributes of northern plants of this foundation species likely resulting in changes in ecosystem productivity.

Beschreibung: This research was made possible by funding provided by NSF/PLR-1417645 to MM. The Botanical Society of America Graduate Student Research Award and the Dodson Research Grant from the Graduate School of the University of Texas at El Paso provided assistance to ES. The grant 5U54MD007592 from the National Institute on Minority Health and Health Disparities (NIMHD), a component of the National Institutes of Health (NIH) provided bioinformatics resources and support of JM.

Beschreibung: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Scalpone, C. R., Jarvis, J. C., Vasslides, J. M., Testa, J. M., & Ganju, N. K. Simulated estuary-wide response of seagrass (Zostera marina) to future scenarios of temperature and sea level. Frontiers in Marine Science, 7, (2020): 539946, doi:10.3389/fmars.2020.539946.

Beschreibung: Seagrass communities are a vital component of estuarine ecosystems, but are threatened by projected sea level rise (SLR) and temperature increases with climate change. To understand these potential effects, we developed a spatially explicit model that represents seagrass (Zostera marina) habitat and estuary-wide productivity for Barnegat Bay-Little Egg Harbor (BB-LEH) in New Jersey, United States. Our modeling approach included an offline coupling of a numerical seagrass biomass model with the spatially variable environmental conditions from a hydrodynamic model to calculate above and belowground biomass at each grid cell of the hydrodynamic model domain. Once calibrated to represent present day seagrass habitat and estuary-wide annual productivity, we applied combinations of increasing air temperature and sea level following regionally specific climate change projections, enabling analysis of the individual and combined impacts of these variables on seagrass biomass and spatial coverage. Under the SLR scenarios, the current model domain boundaries were maintained, as the land surrounding BB-LEH is unlikely to shift significantly in the future. SLR caused habitat extent to decrease dramatically, pushing seagrass beds toward the coastline with increasing depth, with a 100% loss of habitat by the maximum SLR scenario. The dramatic loss of seagrass habitat under SLR was in part due to the assumption that surrounding land would not be inundated, as the model did not allow for habitat expansion outside the current boundaries of the bay. Temperature increases slightly elevated the rate of summer die-off and decreased habitat area only under the highest temperature increase scenarios. In combined scenarios, the effects of SLR far outweighed the effects of temperature increase. Sensitivity analysis of the model revealed the greatest sensitivity to changes in parameters affecting light limitation and seagrass mortality, but no sensitivity to changes in nutrient limitation constants. The high vulnerability of seagrass in the bay to SLR exceeded that demonstrated for other systems, highlighting the importance of site- and region-specific assessments of estuaries under climate change.

Beschreibung: This research was supported by the National Science Foundation Research Experience for Undergraduates Program (OCE-1659463), the Woods Hole Oceanographic Institution Summer Student Fellowship Program, the Barnegat Bay Partnership (through a US EPA Clean Water Act grant to Ocean County College; CE98212313), and the USGS Coastal and Marine Hazards/Resources Program. Although this project has been funded in part by the United States Environmental Protection Agency pursuant to a grant agreement with Ocean County College, it has not gone through the Agency’s publications review process and may not necessarily reflect the views of the Agency; therefore, no official endorsement should be assumed. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Grearson, A. G., Dugan, A., Sakmar, T., Sivitilli, D. M., Gire, D. H., Caldwell, R. L., Niell, C. M., Doelen, G., Wang, Z. Y., & Grasse, B. The lesser Pacific Striped Octopus, Octopus chierchiae: an emerging laboratory model. Frontiers in Marine Science, 8, (2021): 753483, https://doi.org/10.3389/fmars.2021.753483.

Beschreibung: Cephalopods have the potential to become useful experimental models in various fields of science, particularly in neuroscience, physiology, and behavior. Their complex nervous systems, intricate color- and texture-changing body patterns, and problem-solving abilities have attracted the attention of the biological research community, while the high growth rates and short life cycles of some species render them suitable for laboratory culture. Octopus chierchiae is a small octopus native to the central Pacific coast of North America whose predictable reproduction, short time to maturity, small adult size, and ability to lay multiple egg clutches (iteroparity) make this species ideally suited to laboratory culture. Here we describe novel methods for multigenerational culture of O. chierchiae, with emphasis on enclosure designs, feeding regimes, and breeding management. O. chierchiae bred in the laboratory grow from a 3.5 mm mantle length at hatching to an adult mantle length of approximately 20–30 mm in 250–300 days, with 15 and 14% survivorship to over 400 days of age in first and second generations, respectively. O. chierchiae sexually matures at around 6 months of age and, unlike most octopus species, can lay multiple clutches of large, direct-developing eggs every ∼30–90 days. Based on these results, we propose that O. chierchiae possesses both the practical and biological features needed for a model octopus that can be cultured repeatedly to address a wide range of biological questions.

Beschreibung: The cephalopod program at the Marine Biological Laboratory (MBL) was supported by NSF 1827509 and NSF 1723141 grants. CN received funding from HFSP RGP0042. DG and DS received funding and research support from the University of Washington Friday Harbor Laboratories. ZYW was supported by funds from the Whitman Center at the MBL.

Beschreibung: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Castagno, K., Ganju, N., Beck, M., Bowden, A., & Scyphers, S. How much marsh restoration is enough to deliver wave attenuation coastal protection benefits? Frontiers in Marine Science, 8, (2022): 756670, https://doi.org/10.3389/fmars.2021.756670.

Beschreibung: As coastal communities grow more vulnerable to sea-level rise and increased storminess, communities have turned to nature-based solutions to bolster coastal resilience and protection. Marshes have significant wave attenuation properties and can play an important role in coastal protection for many communities. Many restoration projects seek to maximize this ecosystem service but how much marsh restoration is enough to deliver measurable coastal protection benefits is still unknown. This question is critical to guiding assessments of cost effectiveness and for funding, implementation, and optimizing of marsh restoration for risk reduction projects. This study uses SWAN model simulations to determine empirical relationships between wave attenuation and marsh vegetation. The model runs consider several different common marsh morphologies (including systems with channels, ponds, and fringing mudflats), vegetation placement, and simulated storm intensity. Up to a 95% reduction in wave energy is seen at as low as 50% vegetation cover. Although these empirical relationships between vegetative cover and wave attenuation provide essential insight for marsh restoration, it is also important to factor in lifespan estimates of restored marshes when making overall restoration decisions. The results of this study are important for coastal practitioners and managers seeking performance goals and metrics for marsh restoration, enhancement, and creation.

Beschreibung: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in O’Brien, J., McParland, E. L., Bramucci, A. R., Ostrowski, M., Siboni, N., Ingleton, T., Brown, M. V., Levine, N. M., Laverock, B., Petrou, K., & Seymour, J. The microbiological drivers of temporally dynamic Dimethylsulfoniopropionate cycling processes in Australian coastal shelf waters. Frontiers in Microbiology, 13, (2022): 894026, https://doi.org/10.3389/fmicb.2022.894026.

Beschreibung: The organic sulfur compounds dimethylsulfoniopropionate (DMSP) and dimethyl sulfoxide (DMSO) play major roles in the marine microbial food web and have substantial climatic importance as sources and sinks of dimethyl sulfide (DMS). Seasonal shifts in the abundance and diversity of the phytoplankton and bacteria that cycle DMSP are likely to impact marine DMS (O) (P) concentrations, but the dynamic nature of these microbial interactions is still poorly resolved. Here, we examined the relationships between microbial community dynamics with DMS (O) (P) concentrations during a 2-year oceanographic time series conducted on the east Australian coast. Heterogenous temporal patterns were apparent in chlorophyll a (chl a) and DMSP concentrations, but the relationship between these parameters varied over time, suggesting the phytoplankton and bacterial community composition were affecting the net DMSP concentrations through differential DMSP production and degradation. Significant increases in DMSP were regularly measured in spring blooms dominated by predicted high DMSP-producing lineages of phytoplankton (Heterocapsa, Prorocentrum, Alexandrium, and Micromonas), while spring blooms that were dominated by predicted low DMSP-producing phytoplankton (Thalassiosira) demonstrated negligible increases in DMSP concentrations. During elevated DMSP concentrations, a significant increase in the relative abundance of the key copiotrophic bacterial lineage Rhodobacterales was accompanied by a three-fold increase in the gene, encoding the first step of DMSP demethylation (dmdA). Significant temporal shifts in DMS concentrations were measured and were significantly correlated with both fractions (0.2–2 μm and 〉2 μm) of microbial DMSP lyase activity. Seasonal increases of the bacterial DMSP biosynthesis gene (dsyB) and the bacterial DMS oxidation gene (tmm) occurred during the spring-summer and coincided with peaks in DMSP and DMSO concentration, respectively. These findings, along with significant positive relationships between dsyB gene abundance and DMSP, and tmm gene abundance with DMSO, reinforce the significant role planktonic bacteria play in producing DMSP and DMSO in ocean surface waters. Our results highlight the highly dynamic nature and myriad of microbial interactions that govern sulfur cycling in coastal shelf waters and further underpin the importance of microbial ecology in mediating important marine biogeochemical processes.

Beschreibung: This research was supported by the Australian Research Council Grants FT130100218 and DP180100838 awarded to JS and DP140101045 awarded to JS and KP, as well as an Australian Government Research Training Program Scholarship awarded to JO’B.

Beschreibung: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Lasek-Nesselquist, E., & Johnson, M. D. A phylogenomic approach to clarifying the relationship of Mesodinium within the Ciliophora: a case study in the complexity of mixed-species transcriptome analyses. Genome Biology and Evolution, 11(11), (2019): 3218–3232, doi:10.1093/gbe/evz233.

Beschreibung: Recent high-throughput sequencing endeavors have yielded multigene/protein phylogenies that confidently resolve several inter- and intra-class relationships within the phylum Ciliophora. We leverage the massive sequencing efforts from the Marine Microbial Eukaryote Transcriptome Sequencing Project, other SRA submissions, and available genome data with our own sequencing efforts to determine the phylogenetic position of Mesodinium and to generate the most taxonomically rich phylogenomic ciliate tree to date. Regardless of the data mining strategy, the multiprotein data set, or the molecular models of evolution employed, we consistently recovered the same well-supported relationships among ciliate classes, confirming many of the higher-level relationships previously identified. Mesodinium always formed a monophyletic group with members of the Litostomatea, with mixotrophic species of Mesodinium—M. rubrum, M. major, and M. chamaeleon—being more closely related to each other than to the heterotrophic member, M. pulex. The well-supported position of Mesodinium as sister to other litostomes contrasts with previous molecular analyses including those from phylogenomic studies that exploited the same transcriptomic databases. These topological discrepancies illustrate the need for caution when mining mixed-species transcriptomes and indicate that identifying ciliate sequences among prey contamination—particularly for Mesodinium species where expression from stolen prey nuclei appears to dominate—requires thorough and iterative vetting with phylogenies that incorporate sequences from a large outgroup of prey.

Beschreibung: We thank David Beaudoin and Holly V. Moeller for their assistance in collecting cells and extracting RNA. We thank the Josephine Bay Paul Center for Comparative Molecular Biology and Evolution at the Marine Biological Laboratory for the generous use of their servers. This work was supported in part by a National Science Foundation grant to both authors (IOS 1354773).

Beschreibung: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Xu, X., Li, G., Li, C., Zhang, J., Wang, Q., Simmons, D. K., Chen, X., Wijesena, N., Zhu, W., Wang, Z., Wang, Z., Ju, B., Ci, W., Lu, X., Yu, D., Wang, Q., Aluru, N., Oliveri, P., Zhang, Y. E., Martindale, M. Q., & Liu, J. Evolutionary transition between invertebrates and vertebrates via methylation reprogramming in embryogenesis. National Science Review, 6(5), (2019):993-1003, doi:10.1093/nsr/nwz064.

Beschreibung: Major evolutionary transitions are enigmas, and the most notable enigma is between invertebrates and vertebrates, with numerous spectacular innovations. To search for the molecular connections involved, we asked whether global epigenetic changes may offer a clue by surveying the inheritance and reprogramming of parental DNA methylation across metazoans. We focused on gametes and early embryos, where the methylomes are known to evolve divergently between fish and mammals. Here, we find that methylome reprogramming during embryogenesis occurs neither in pre-bilaterians such as cnidarians nor in protostomes such as insects, but clearly presents in deuterostomes such as echinoderms and invertebrate chordates, and then becomes more evident in vertebrates. Functional association analysis suggests that DNA methylation reprogramming is associated with development, reproduction and adaptive immunity for vertebrates, but not for invertebrates. Interestingly, the single HOX cluster of invertebrates maintains unmethylated status in all stages examined. In contrast, the multiple HOX clusters show dramatic dynamics of DNA methylation during vertebrate embryogenesis. Notably, the methylation dynamics of HOX clusters are associated with their spatiotemporal expression in mammals. Our study reveals that DNA methylation reprogramming has evolved dramatically during animal evolution, especially after the evolutionary transitions from invertebrates to vertebrates, and then to mammals.

Beschreibung: This work was supported by the National Key Research and Development Program of China (2018YFC1003303), the Strategic Priority Research Program of the CAS (XDB13040200), the National Natural Science Foundation of China (91519306, 31425015), the Youth Innovation Promotion Association of the CAS and the Key Research Program of Frontier Sciences, CAS (QYZDY-SSW-SMC016).

Beschreibung: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Vallecillo-Viejo, I. C., Liscovitch-Brauer, N., Diaz Quiroz, J. F., Montiel-Gonzalez, Maria F., Nemes, Sonya E., Rangan, K. J., Levinson, S. R., Eisenberg, E., & Rosenthal, J. J. C. Spatially regulated editing of genetic information within a neuron. Nucleic Acids Research, (2020): gkaa172, doi: 10.1093/nar/gkaa172.

Beschreibung: In eukaryotic cells, with the exception of the specialized genomes of mitochondria and plastids, all genetic information is sequestered within the nucleus. This arrangement imposes constraints on how the information can be tailored for different cellular regions, particularly in cells with complex morphologies like neurons. Although messenger RNAs (mRNAs), and the proteins that they encode, can be differentially sorted between cellular regions, the information itself does not change. RNA editing by adenosine deamination can alter the genome’s blueprint by recoding mRNAs; however, this process too is thought to be restricted to the nucleus. In this work, we show that ADAR2 (adenosine deaminase that acts on RNA), an RNA editing enzyme, is expressed outside of the nucleus in squid neurons. Furthermore, purified axoplasm exhibits adenosine-to-inosine activity and can specifically edit adenosines in a known substrate. Finally, a transcriptome-wide analysis of RNA editing reveals that tens of thousands of editing sites (〉70% of all sites) are edited more extensively in the squid giant axon than in its cell bodies. These results indicate that within a neuron RNA editing can recode genetic information in a region-specific manner.

Beschreibung: National Science Foundation (NSF) [IOS1557748 to J.R.]; United States–Israel Binational Science Foundation [BSF2013094 to J.R. and E.E.]; The Grass Foundation grant in support of the Doryteuthis pealeii Genome Project, and a gift by Mr. Edward Owens. Funding for open access charge: United States–Israel Binational Science Foundation [BSF2013094].

Beschreibung: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Mayers, K. M. J., Poulton, A. J., Bidle, K., Thamatrakoln, K., Schieler, B., Giering, S. L. C., Wells, S. R., Tarran, G. A., Mayor, D., Johnson, M., Riebesell, U., Larsen, A., Vardi, A., & Harvey, E. L. The possession of coccoliths fails to deter microzooplankton grazers. Frontiers in Marine Science, 7, (2020): 562020, doi:10.3389/fmars.2020.569896.

Beschreibung: Phytoplankton play a central role in the regulation of global carbon and nutrient cycles, forming the basis of the marine food webs. A group of biogeochemically important phytoplankton, the coccolithophores, produce calcium carbonate scales that have been hypothesized to deter or reduce grazing by microzooplankton. Here, a meta-analysis of mesocosm-based experiments demonstrates that calcification of the cosmopolitan coccolithophore, Emiliania huxleyi, fails to deter microzooplankton grazing. The median grazing to growth ratio for E. huxleyi (0.56 ± 0.40) was not significantly different among non-calcified nano- or picoeukaryotes (0.71 ± 0.31 and 0.55 ± 0.34, respectively). Additionally, the environmental concentration of E. huxleyi did not drive preferential grazing of non-calcified groups. These results strongly suggest that the possession of coccoliths does not provide E. huxleyi effective protection from microzooplankton grazing. Such indiscriminate consumption has implications for the dissolution and fate of CaCO3 in the ocean, and the evolution of coccoliths.

Beschreibung: Mesocosm experiments in 2015 were supported by the Kiel Excellence Cluster “The Future Ocean” (CP1540) and the Leibniz Award to UR, in 2017 the MESOHUX experiment was supported by NSF (OCE-1559179) to KT and KB, NSF (OCE-1537951 and OCE-1459200) to KB, NSF (OCE-1459190, 1657808, and DBI-1624593) to EH, and in 2018 by AQUACOSM (EU H2020-INFRAIA-project No 731065). KM was supported by a NERC Doctoral Training Partnership (DTP) studentship as part of the Southampton Partnership for Innovative Training of Future Investigators Researching the Environment (SPITFIRE, grant number NE/L002531/1) and Research Council of Norway project (#280414) MIXsTRUCT.

Beschreibung: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Wong, A. P. S., Wijffels, S. E., Riser, S. C., Pouliquen, S., Hosoda, S., Roemmich, D., Gilson, J., Johnson, G. C., Martini, K., Murphy, D. J., Scanderbeg, M., Bhaskar, T. V. S. U., Buck, J. J. H., Merceur, F., Carval, T., Maze, G., Cabanes, C., Andre, X., Poffa, N., Yashayaev, I., Barker, P. M., Guinehut, S., Belbeoch, M., Ignaszewski, M., Baringer, M. O., Schmid, C., Lyman, J. M., McTaggart, K. E., Purkey, S. G., Zilberman, N., Alkire, M. B., Swift, D., Owens, W. B., Jayne, S. R., Hersh, C., Robbins, P., West-Mack, D., Bahr, F., Yoshida, S., Sutton, P. J. H., Cancouet, R., Coatanoan, C., Dobbler, D., Juan, A. G., Gourrion, J., Kolodziejczyk, N., Bernard, V., Bourles, B., Claustre, H., D'Ortenzio, F., Le Reste, S., Le Traon, P., Rannou, J., Saout-Grit, C., Speich, S., Thierry, V., Verbrugge, N., Angel-Benavides, I. M., Klein, B., Notarstefano, G., Poulain, P., Velez-Belchi, P., Suga, T., Ando, K., Iwasaska, N., Kobayashi, T., Masuda, S., Oka, E., Sato, K., Nakamura, T., Sato, K., Takatsuki, Y., Yoshida, T., Cowley, R., Lovell, J. L., Oke, P. R., van Wijk, E. M., Carse, F., Donnelly, M., Gould, W. J., Gowers, K., King, B. A., Loch, S. G., Mowat, M., Turton, J., Rama Rao, E. P., Ravichandran, M., Freeland, H. J., Gaboury, I., Gilbert, D., Greenan, B. J. W., Ouellet, M., Ross, T., Tran, A., Dong, M., Liu, Z., Xu, J., Kang, K., Jo, H., Kim, S., & Park, H. Argo data 1999-2019: two million temperature-salinity profiles and subsurface velocity observations from a global array of profiling floats. Frontiers in Marine Science, 7, (2020): 700, doi:10.3389/fmars.2020.00700.

Beschreibung: In the past two decades, the Argo Program has collected, processed, and distributed over two million vertical profiles of temperature and salinity from the upper two kilometers of the global ocean. A similar number of subsurface velocity observations near 1,000 dbar have also been collected. This paper recounts the history of the global Argo Program, from its aspiration arising out of the World Ocean Circulation Experiment, to the development and implementation of its instrumentation and telecommunication systems, and the various technical problems encountered. We describe the Argo data system and its quality control procedures, and the gradual changes in the vertical resolution and spatial coverage of Argo data from 1999 to 2019. The accuracies of the float data have been assessed by comparison with high-quality shipboard measurements, and are concluded to be 0.002°C for temperature, 2.4 dbar for pressure, and 0.01 PSS-78 for salinity, after delayed-mode adjustments. Finally, the challenges faced by the vision of an expanding Argo Program beyond 2020 are discussed.

Beschreibung: AW, SR, and other scientists at the University of Washington (UW) were supported by the US Argo Program through the NOAA Grant NA15OAR4320063 to the Joint Institute for the Study of the Atmosphere and Ocean (JISAO) at the UW. SW and other scientists at the Woods Hole Oceanographic Institution (WHOI) were supported by the US Argo Program through the NOAA Grant NA19OAR4320074 (CINAR/WHOI Argo). The Scripps Institution of Oceanography's role in Argo was supported by the US Argo Program through the NOAA Grant NA15OAR4320071 (CIMEC). Euro-Argo scientists were supported by the Monitoring the Oceans and Climate Change with Argo (MOCCA) project, under the Grant Agreement EASME/EMFF/2015/1.2.1.1/SI2.709624 for the European Commission.

Beschreibung: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Marian, J. E. A. R., Apostólico, L. H., Chiao, C. C., Hanlon, R. T., Hirohashi, N., Iwata, Y., Mather, J., Sato, N., & Shaw, P. W. Male alternative reproductive tactics and associated evolution of anatomical characteristics in loliginid squid. Frontiers in Physiology, 10, (2019): 1281, doi: 10.3389/fphys.2019.01281.

Beschreibung: Loliginid squids provide a unique model system to explore male alternative reproductive tactics (ARTs) and their linkage to size, behavioral decision making, and possibly age. Large individuals fight one another and the winners form temporary consortships with females, while smaller individuals do not engage in male-male agonistic bouts but use various sneaker tactics to obtain matings, each with varying mating and fertilization success. There is substantial behavioral flexibility in most species, as smaller males can facultatively switch to the alternative consort behaviors as the behavioral context changes. These forms of ARTs can involve different: mating posture; site of spermatophore deposition; fertilization success; and sperm traits. Most of the traits of male dimorphism (both anatomical and behavioral) are consistent with traditional sexual selection theory, while others have unique features that may have evolved in response to the fertilization environment faced by each temporary or permanent male morph.

Beschreibung: JM acknowledges the funding provided by FAPESP (São Paulo Research Foundation – proc. 2013/02653-1, 2014/11008-5, 2015/15447-6, 2017/16182-1, and 2018/19180-2), CNPq (National Council for Scientific and Technological Development – proc. 477233/2013–9), and CAPES (Coordination for the Improvement of Higher Education Personnel – Finance Code 001).

Beschreibung: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Soll, L. G., Eisen, J. N., Vargas, K. J., Medeiros, A. T., Hammar, K. M., & Morgan, J. R. α-Synuclein-112 impairs synaptic vesicle recycling consistent with its enhanced membrane binding properties. Frontiers in Cell and Developmental Biology, 8, (2020): 405, doi:10.3389/fcell.2020.00405.

Beschreibung: Synucleinopathies are neurological disorders associated with α-synuclein overexpression and aggregation. While it is well-established that overexpression of wild type α-synuclein (α-syn-140) leads to cellular toxicity and neurodegeneration, much less is known about other naturally occurring α-synuclein splice isoforms. In this study we provide the first detailed examination of the synaptic effects caused by one of these splice isoforms, α-synuclein-112 (α-syn-112). α-Syn-112 is produced by an in-frame excision of exon 5, resulting in deletion of amino acids 103–130 in the C-terminal region. α-Syn-112 is upregulated in the substantia nigra, frontal cortex, and cerebellum of parkinsonian brains and higher expression levels are correlated with susceptibility to Parkinson’s disease (PD), dementia with Lewy bodies (DLB), and multiple systems atrophy (MSA). We report here that α-syn-112 binds strongly to anionic phospholipids when presented in highly curved liposomes, similar to α-syn-140. However, α-syn-112 bound significantly stronger to all phospholipids tested, including the phosphoinositides. α-Syn-112 also dimerized and trimerized on isolated synaptic membranes, while α-syn-140 remained largely monomeric. When introduced acutely to lamprey synapses, α-syn-112 robustly inhibited synaptic vesicle recycling. Interestingly, α-syn-112 produced effects on the plasma membrane and clathrin-mediated synaptic vesicle endocytosis that were phenotypically intermediate between those caused by monomeric and dimeric α-syn-140. These findings indicate that α-syn-112 exhibits enhanced phospholipid binding and oligomerization in vitro and consequently interferes with synaptic vesicle recycling in vivo in ways that are consistent with its biochemical properties. This study provides additional evidence suggesting that impaired vesicle endocytosis is a cellular target of excess α-synuclein and advances our understanding of potential mechanisms underlying disease pathogenesis in the synucleinopathies.

Beschreibung: This study was supported by a research grant from the National Institutes of Health (NIH NINDS/NIA R01 NS078165 to JM), as well as research funds from the Marine Biological Laboratory (to JM).

Beschreibung: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in da Fonseca, R. R., Couto, A., Machado, A. M., Brejova, B., Albertin, C. B., Silva, F., Gardner, P., Baril, T., Hayward, A., Campos, A., Ribeiro, A. M., Barrio-Hernandez, I., Hoving, H. J., Tafur-Jimenez, R., Chu, C., Frazao, B., Petersen, B., Penaloza, F., Musacchia, F., Alexander, G. C., Osorio, H., Winkelmann, I., Simakov, O., Rasmussen, S., Rahman, M. Z., Pisani, D., Vinther, J., Jarvis, E., Zhang, G., Strugnell, J. M., Castro, L. F. C., Fedrigo, O., Patricio, M., Li, Q., Rocha, S., Antunes, A., Wu, Y., Ma, B., Sanges, R., Vinar, T., Blagoev, B., Sicheritz-Ponten, T., Nielsen, R., & Gilbert, M. T. P. A draft genome sequence of the elusive giant squid, Architeuthis dux. Gigascience, 9(1), (2020): giz152. doi: 10.1093/gigascience/giz152.

Beschreibung: Background: The giant squid (Architeuthis dux; Steenstrup, 1857) is an enigmatic giant mollusc with a circumglobal distribution in the deep ocean, except in the high Arctic and Antarctic waters. The elusiveness of the species makes it difficult to study. Thus, having a genome assembled for this deep-sea–dwelling species will allow several pending evolutionary questions to be unlocked. Findings: We present a draft genome assembly that includes 200 Gb of Illumina reads, 4 Gb of Moleculo synthetic long reads, and 108 Gb of Chicago libraries, with a final size matching the estimated genome size of 2.7 Gb, and a scaffold N50 of 4.8 Mb. We also present an alternative assembly including 27 Gb raw reads generated using the Pacific Biosciences platform. In addition, we sequenced the proteome of the same individual and RNA from 3 different tissue types from 3 other species of squid (Onychoteuthis banksii, Dosidicus gigas, and Sthenoteuthis oualaniensis) to assist genome annotation. We annotated 33,406 protein-coding genes supported by evidence, and the genome completeness estimated by BUSCO reached 92%. Repetitive regions cover 49.17% of the genome. Conclusions: This annotated draft genome of A. dux provides a critical resource to investigate the unique traits of this species, including its gigantism and key adaptations to deep-sea environments.

Beschreibung: R.R.F. thanks the Villum Fonden for grant VKR023446 (Villum Fonden Young Investigator Grant), the Portuguese Science Foundation (FCT) for grant PTDC/MAR/115347/2009; COMPETE-FCOMP-01-012; FEDER-015453, Marie Curie Actions (FP7-PEOPLE-2010-IEF, Proposal 272927), and the Danish National Research Foundation (DNRF96) for its funding of the Center for Macroecology, Evolution, and Climate. H.O. thanks the Rede Nacional de Espectrometria de Massa, ROTEIRO/0028/2013, ref. LISBOA-01-0145-FEDER-022125, supported by COMPETE and North Portugal Regional Operational Programme (Norte2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). A.C. thanks FCT for project UID/Multi/04423/2019. M.P. acknowledges the support from the Wellcome Trust (grant number WT108749/Z/15/Z) and the European Molecular Biology Laboratory. M.P.T.G. thanks the Danish National Research Foundation for its funding of the Center for GeoGenetics (grant DNRF94) and Lundbeck Foundation for grant R52–5062 on Pathogen Palaeogenomics. S.R. was supported by the Novo Nordisk Foundation grant NNF14CC0001. A.H. is supported by a Biotechnology and Biological Sciences Research Council David Phillips Fellowship (fellowship reference: BB/N020146/1). T.B. is supported by the Biotechnology and Biological Sciences Research Council-funded South West Biosciences Doctoral Training Partnership (training grant reference BB/M009122/1). This work was partially funded by the Lundbeck Foundation (R52-A4895 to B.B.). H.J.T.H. was supported by the David and Lucile Packard Foundation, the Netherlands Organization for Scientific Research (#825.09.016), and currently by the Deutsche Forschungsgemeinschaft (DFG) under grant HO 5569/2-1 (Emmy Noether Junior Research Group). T.V. and B. Brejova were supported by grants from the Slovak grant agency VEGA (1/0684/16, 1/0458/18). F.S. was supported by a PhD grant (SFRH/BD/126560/2016) from FCT. A.A. was partly supported by the FCT project PTDC/CTA-AMB/31774/2017. C.C. and Y.W. are partly supported by grant IIS-1526415 from the US National Science Foundation. Computation for the work described in this article was partially supported by the DeiC National Life Science Supercomputer at DTU.

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Costa Jr, C., Galford, G. L., Coe, M. T., Macedo, M., Jankowski, K., O’Connell, C., & Neill, C. Modeling nitrous oxide emissions from large-scale intensive cropping systems in the southern Amazon. Frontiers in Sustainable Food Systems, 5, (2021): 701416. https://doi.org/10.3389/fsufs.2021.701416.

Beschreibung: Nitrogen (N) fertilizer use is rapidly intensifying on tropical croplands and has the potential to increase emissions of the greenhouse gas, nitrous oxide (N2O). Since about 2005 Mato Grosso (MT), Brazil has shifted from single-cropped soybeans to double-cropping soybeans with maize, and now produces 1.5% of the world's maize. This production shift required an increase in N fertilization, but the effects on N2O emissions are poorly known. We calibrated the process-oriented biogeochemical DeNitrification-DeComposition (DNDC) model to simulate N2O emissions and crop production from soybean and soybean-maize cropping systems in MT. After model validation with field measurements and adjustments for hydrological properties of tropical soils, regional simulations suggested N2O emissions from soybean-maize cropland increased almost fourfold during 2001–2010, from 1.1 ± 1.1 to 4.1 ± 3.2 Gg 1014 N-N2O. Model sensitivity tests showed that emissions were spatially and seasonably variable and especially sensitive to soil bulk density and carbon content. Meeting future demand for maize using current soybean area in MT might require either (a) intensifying 3.0 million ha of existing single soybean to soybean-maize or (b) increasing N fertilization to ~180 kg N ha−1 on existing 2.3 million ha of soybean-maize area. The latter strategy would release ~35% more N2O than the first. Our modifications of the DNDC model will improve estimates of N2O emissions from agricultural production in MT and other tropical areas, but narrowing model uncertainty will depend on more detailed field measurements and spatial data on soil and cropping management.

Beschreibung: This work was supported by National Science Foundation (NSF#1257944) and CNPq-Ciências Sem Fronteiras Post-Doctoral Fellowship (249380/2013-7).

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Morris, S. L., Tsai, M., Aloe, S., Bechberger, K., Konig, S., Morfini, G., & Brady, S. T. Defined tau phosphospecies differentially inhibit fast axonal transport through activation of two independent signaling pathways. Frontiers in Molecular Neuroscience, 13, (2021): 610037, https://doi.org/10.3389/fnmol.2020.610037.

Beschreibung: Tau protein is subject to phosphorylation by multiple kinases at more than 80 different sites. Some of these sites are associated with tau pathology and neurodegeneration, but other sites are modified in normal tau as well as in pathological tau. Although phosphorylation of tau at residues in the microtubule-binding repeats is thought to reduce tau association with microtubules, the functional consequences of other sites are poorly understood. The AT8 antibody recognizes a complex phosphoepitope site on tau that is detectable in a healthy brain but significantly increased in Alzheimer’s disease (AD) and other tauopathies. Previous studies showed that phosphorylation of tau at the AT8 site leads to exposure of an N-terminal sequence that promotes activation of a protein phosphatase 1 (PP1)/glycogen synthase 3 (GSK3) signaling pathway, which inhibits kinesin-1-based anterograde fast axonal transport (FAT). This finding suggests that phosphorylation may control tau conformation and function. However, the AT8 includes three distinct phosphorylated amino acids that may be differentially phosphorylated in normal and disease conditions. To evaluate the effects of specific phosphorylation sites in the AT8 epitope, recombinant, pseudophosphorylated tau proteins were perfused into the isolated squid axoplasm preparation to determine their effects on axonal signaling pathways and FAT. Results from these studies suggest a mechanism where specific phosphorylation events differentially impact tau conformation, promoting activation of independent signaling pathways that differentially affect FAT. Implications of findings here to our understanding of tau function in health and disease conditions are discussed.

Beschreibung: This research was funded by NIH grants R21NS096642 (GM); 1R01NS118177-01A1 (GM), R01 NS082730 (SB), a Zenith Award from the Alzheimer’s Association (SB), and a grant from the Tau Consortium/Rainwater Foundation (SB).

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Pedrosa-Pamies, R., Parinos, C., Sanchez-Vidal, A., Calafat, A., Canals, M., Velaoras, D., Mihalopoulos, N., Kanakidou, M., Lampadariou, N., & Gogou, A. Atmospheric and oceanographic forcing impact particle flux composition and carbon sequestration in the eastern Mediterranean Sea: a three-year time-series study in the deep Ierapetra Basin. Frontiers in Earth Science, 9, (2021): 591948, https://doi.org/10.3389/feart.2021.591948.

Beschreibung: Sinking particles are a critical conduit for the export of organic material from surface waters to the deep ocean. Despite their importance in oceanic carbon cycling, little is known about the biotic composition and seasonal variability of sinking particles reaching abyssal depths. Herein, sinking particle flux data, collected in the deep Ierapetra Basin for a three-year period (June 2010 to June 2013), have been examined at the light of atmospheric and oceanographic parameters and main mass components (lithogenic, opal, carbonates, nitrogen, and organic carbon), stable isotopes of particulate organic carbon (POC) and source-specific lipid biomarkers. Our aim is to improve the current understanding of the dynamics of particle fluxes and the linkages between atmospheric dynamics and ocean biogeochemistry shaping the export of organic matter in the deep Eastern Mediterranean Sea. Overall, particle fluxes showed seasonality and interannual variability over the studied period. POC fluxes peaked in spring April–May 2012 (12.2 mg m−2 d−1) related with extreme atmospheric forcing. Summer export was approximately fourfold higher than mean wintertime, fall and springtime (except for the episodic event of spring 2012), fueling efficient organic carbon sequestration. Lipid biomarkers indicate a high relative contribution of natural and anthropogenic, marine- and land-derived POC during both spring (April–May) and summer (June–July) reaching the deep-sea floor. Moreover, our results highlight that both seasonal and episodic pulses are crucial for POC export, while the coupling of extreme weather events and atmospheric deposition can trigger the influx of both marine labile carbon and anthropogenic compounds to the deep Levantine Sea. Finally, the comparison of time series data of sinking particulate flux with the corresponding biogeochemical parameters data previously reported for surface sediment samples from the deep-sea shed light on the benthic–pelagic coupling in the study area. Thus, this study underscores that accounting the seasonal and episodic pulses of organic carbon into the deep sea is critical in modeling the depth and intensity of natural and anthropogenic POC sequestration, and for a better understanding of the global carbon cycle.

Beschreibung: This research was supported by the REDECO (CTM2008-04973-E/MAR) and PERSEUS (GA 287600) projects. We further acknowledge support by the projects PANACEA—‘PANhellenic infrastructure for Atmospheric Composition and climatE chAnge’ (MIS 5021516) and ENIRISST—‘Intelligent Research Infrastructure for Shipping, Supply Chain, Transport and Logistics’ (MIS 5027930), which are implemented under the Action “Reinforcement of the Research and Innovation Infrastructure,” funded by the Operational Program “Competitiveness, Entrepreneurship and Innovation” (NSRF 2014-2020) and co-financed by Greece and EU; and by the Action “National Νetwork on Climate Change and its Impacts - Climpact” which is implemented under the sub-project 3 of the project “Infrastructure of national research networks in the fields of Precision Medicine, Quantum Technology and Climate Change,” funded by the Public Investment Program of Greece, General Secretary of Research and Technology/Ministry of Development and Investments.” Researchers from GRC Geociències Marines benefited from a Grups de Recerca Consolidats grant (2017 SGR 315) by Generalitat de Catalunya autonomous government.

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Kalra, T. S., Ganju, N. K., Aretxabaleta, A. L., Carr, J. A., Defne, Z., & Moriarty, J. M. Modeling marsh dynamics using a 3-D coupled wave-flow-sediment model. Frontiers in Marine Science, 8, (2021): 740921, https://doi.org/10.3389/fmars.2021.740921.

Beschreibung: Salt marshes are dynamic biogeomorphic systems that respond to external physical factors, including tides, sediment transport, and waves, as well as internal processes such as autochthonous soil formation. Predicting the fate of marshes requires a modeling framework that accounts for these processes in a coupled fashion. In this study, we implement two new marsh dynamic processes in the 3-D COAWST (coupled-ocean-atmosphere-wave sediment transport) model. The processes added are the erosion of the marsh edge scarp caused by lateral wave thrust from surface waves and vertical accretion driven by biomass production on the marsh platform. The sediment released from the marsh during edge erosion causes a change in bathymetry, thereby modifying the wave-energy reaching the marsh edge. Marsh vertical accretion due to biomass production is considered for a single vegetation species and is determined by the hydroperiod parameters (tidal datums) and the elevation of the marsh cells. Tidal datums are stored at user-defined intervals as a hindcast (on the order of days) and used to update the vertical growth formulation. Idealized domains are utilized to verify the lateral wave thrust formulation and show the dynamics of lateral wave erosion leading to horizontal retreat of marsh edge. The simulations of Reedy and Dinner Creeks within the Barnegat Bay estuary system demonstrate the model capability to account for both lateral wave erosion and vertical accretion due to biomass production in a realistic marsh complex. The simulations show that vertical accretion is dominated by organic deposition in the marsh interior, whereas deposition of mineral estuarine sediments occurs predominantly along the channel edges. The ability of the model to capture the fate of the sediment can be extended to model to simulate the impacts of future storms and relative sea-level rise (RSLR) scenarios on salt-marsh ecomorphodynamics.

Beschreibung: This work was supported by USGS Coastal and Marine Hazards and Resources Program.

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Wigand, C., Oczkowski, A. J., Branoff, B. L., Eagle, M., Hanson, A., Martin, R. M., Balogh, S., Miller, K. M., Huertas, E., Loffredo, J., & Watson, E. B. Recent nitrogen storage and accumulation rates in mangrove soils exceed historic rates in the urbanized San Juan Bay Estuary (Puerto Rico, United States). Frontiers in Forests and Global Change, 4, (2021): 765896, https://doi.org/10.3389/ffgc.2021.765896.

Beschreibung: Tropical mangrove forests have been described as “coastal kidneys,” promoting sediment deposition and filtering contaminants, including excess nutrients. Coastal areas throughout the world are experiencing increased human activities, resulting in altered geomorphology, hydrology, and nutrient inputs. To effectively manage and sustain coastal mangroves, it is important to understand nitrogen (N) storage and accumulation in systems where human activities are causing rapid changes in N inputs and cycling. We examined N storage and accumulation rates in recent (1970 – 2016) and historic (1930 – 1970) decades in the context of urbanization in the San Juan Bay Estuary (SJBE, Puerto Rico), using mangrove soil cores that were radiometrically dated. Local anthropogenic stressors can alter N storage rates in peri-urban mangrove systems either directly by increasing N soil fertility or indirectly by altering hydrology (e.g., dredging, filling, and canalization). Nitrogen accumulation rates were greater in recent decades than historic decades at Piñones Forest and Martin Peña East. Martin Peña East was characterized by high urbanization, and Piñones, by the least urbanization in the SJBE. The mangrove forest at Martin Peña East fringed a poorly drained canal and often received raw sewage inputs, with N accumulation rates ranging from 17.7 to 37.9 g m–2 y–1 in recent decades. The Piñones Forest was isolated and had low flushing, possibly exacerbated by river damming, with N accumulation rates ranging from 18.6 to 24.2 g m–2 y–1 in recent decades. Nearly all (96.3%) of the estuary-wide mangrove N (9.4 Mg ha–1) was stored in the soils with 7.1 Mg ha–1 sequestered during 1970–2017 (0–18 cm) and 2.3 Mg ha–1 during 1930–1970 (19–28 cm). Estuary-wide mangrove soil N accumulation rates were over twice as great in recent decades (0.18 ± 0.002 Mg ha–1y–1) than historically (0.08 ± 0.001 Mg ha–1y–1). Nitrogen accumulation rates in SJBE mangrove soils in recent times were twofold larger than the rate of human-consumed food N that is exported as wastewater (0.08 Mg ha–1 y–1), suggesting the potential for mangroves to sequester human-derived N. Conservation and effective management of mangrove forests and their surrounding watersheds in the Anthropocene are important for maintaining water quality in coastal communities throughout tropical regions.

Beschreibung: Some funding was provided by the United States Geological Coastal and Marine Hazards and Resources Program.

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Muenzer, P., Negro, R., Fukui, S., di Meglio, L., Aymonnier, K., Chu, L., Cherpokova, D., Gutch, S., Sorvillo, N., Shi, L., Magupalli, V. G., Weber, A. N. R., Scharf, R. E., Waterman, C. M., Wu, H., & Wagner, D. D. NLRP3 inflammasome assembly in neutrophils is supported by PAD4 and promotes NETosis under sterile conditions. Frontiers in Immunology, 12, (2021): 683803, https://doi.org/10.3389/fimmu.2021.683803.

Beschreibung: Neutrophil extracellular trap formation (NETosis) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome assembly are associated with a similar spectrum of human disorders. While NETosis is known to be regulated by peptidylarginine deiminase 4 (PAD4), the role of the NLRP3 inflammasome in NETosis was not addressed. Here, we establish that under sterile conditions the cannonical NLRP3 inflammasome participates in NETosis. We show apoptosis-associated speck-like protein containing a CARD (ASC) speck assembly and caspase-1 cleavage in stimulated mouse neutrophils without LPS priming. PAD4 was needed for optimal NLRP3 inflammasome assembly by regulating NLRP3 and ASC protein levels post-transcriptionally. Genetic ablation of NLRP3 signaling resulted in impaired NET formation, because NLRP3 supported both nuclear envelope and plasma membrane rupture. Pharmacological inhibition of NLRP3 in either mouse or human neutrophils also diminished NETosis. Finally, NLRP3 deficiency resulted in a lower density of NETs in thrombi produced by a stenosis-induced mouse model of deep vein thrombosis. Altogether, our results indicate a PAD4-dependent formation of the NLRP3 inflammasome in neutrophils and implicate NLRP3 in NETosis under noninfectious conditions in vitro and in vivo.

Beschreibung: This work was supported by a grant from National Heart, Lung, and Blood Institute of the National Institutes of Health (grant R35 HL135765) and a Steven Berzin family support to DDW, an Individual Erwin Deutsch fellowship by the German, Austrian and Swiss Society of Thrombosis and Hemostasis Research to RES, a Whitman fellowship (MBL) to DDW, and an Individual Marie Skłodowska-Curie Actions fellowship by the European Commission (796365 - COAGULANT) to PM. ANRW was funded by the Deutsche Forschungsgemeinschaft (TRR156/2 –246807620) and a research grant (We-4195/15-19). CMW was supported by the Division of Intramural Research, NHLBI, NIH.

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Fouke, K. E., Wegman, M. E., Weber, S. A., Brady, E. B., Roman-Vendrell, C., & Morgan, J. R. Synuclein regulates synaptic vesicle clustering and docking at a vertebrate synapse. Frontiers in Cell and Developmental Biology, 9, (2021): 774650, https://doi.org/10.3389/fcell.2021.774650.

Beschreibung: Neurotransmission relies critically on the exocytotic release of neurotransmitters from small synaptic vesicles (SVs) at the active zone. Therefore, it is essential for neurons to maintain an adequate pool of SVs clustered at synapses in order to sustain efficient neurotransmission. It is well established that the phosphoprotein synapsin 1 regulates SV clustering at synapses. Here, we demonstrate that synuclein, another SV-associated protein and synapsin binding partner, also modulates SV clustering at a vertebrate synapse. When acutely introduced to unstimulated lamprey reticulospinal synapses, a pan-synuclein antibody raised against the N-terminal domain of α-synuclein induced a significant loss of SVs at the synapse. Both docked SVs and the distal reserve pool of SVs were depleted, resulting in a loss of total membrane at synapses. In contrast, antibodies against two other abundant SV-associated proteins, synaptic vesicle glycoprotein 2 (SV2) and vesicle-associated membrane protein (VAMP/synaptobrevin), had no effect on the size or distribution of SV clusters. Synuclein perturbation caused a dose-dependent reduction in the number of SVs at synapses. Interestingly, the large SV clusters appeared to disperse into smaller SV clusters, as well as individual SVs. Thus, synuclein regulates clustering of SVs at resting synapses, as well as docking of SVs at the active zone. These findings reveal new roles for synuclein at the synapse and provide critical insights into diseases associated with α-synuclein dysfunction, such as Parkinson’s disease.

Beschreibung: Funding support for this project was provided by the National Institutes of Health NINDS/NIA R01 NS078165 (to JM); University of Chicago Jeff Metcalf Fellowship Grant (to SW).

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Zottoli, S. J., Faber, D. S., Hering, J., Dannhauer, A. C., & Northen, S. Survival and axonal outgrowth of the Mauthner cell following spinal cord crush does not drive post-injury startle responses. Frontiers in Cell and Developmental Biology, 9, (2021): 744191, https://doi.org/10.3389/fcell.2021.744191.

Beschreibung: A pair of Mauthner cells (M-cells) can be found in the hindbrain of most teleost fish, as well as amphibians and lamprey. The axons of these reticulospinal neurons cross the midline and synapse on interneurons and motoneurons as they descend the length of the spinal cord. The M-cell initiates fast C-type startle responses (fast C-starts) in goldfish and zebrafish triggered by abrupt acoustic/vibratory stimuli. Starting about 70 days after whole spinal cord crush, less robust startle responses with longer latencies manifest in adult goldfish, Carassius auratus. The morphological and electrophysiological identifiability of the M-cell provides a unique opportunity to study cellular responses to spinal cord injury and the relation of axonal regrowth to a defined behavior. After spinal cord crush at the spinomedullary junction about one-third of the damaged M-axons of adult goldfish send at least one sprout past the wound site between 56 and 85 days postoperatively. These caudally projecting sprouts follow a more lateral trajectory relative to their position in the fasciculus longitudinalis medialis of control fish. Other sprouts, some from the same axon, follow aberrant pathways that include rostral projections, reversal of direction, midline crossings, neuromas, and projection out the first ventral root. Stimulating M-axons in goldfish that had post-injury startle behavior between 198 and 468 days postoperatively resulted in no or minimal EMG activity in trunk and tail musculature as compared to control fish. Although M-cells can survive for at least 468 day (∼1.3 years) after spinal cord crush, maintain regrowth, and elicit putative trunk EMG responses, the cell does not appear to play a substantive role in the emergence of acoustic/vibratory-triggered responses. We speculate that aberrant pathway choice of this neuron may limit its role in the recovery of behavior and discuss structural and functional properties of alternative candidate neurons that may render them more supportive of post-injury startle behavior.

Beschreibung: Support for this research came in part from NSF grant (BNS 8809445), NIH grant (2-P01-NS24707-09), and HHMI and Essel Foundation grants to Williams College.

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Tassia, M. G., David, K. T., Townsend, J. P., & Halanych, K. M. TIAMMAt: leveraging biodiversity to revise protein domain models, evidence from innate immunity. Molecular Biology and Evolution, 38(12), (2021): 5806–5818, https://doi.org/10.1093/molbev/msab258.

Beschreibung: Sequence annotation is fundamental for studying the evolution of protein families, particularly when working with nonmodel species. Given the rapid, ever-increasing number of species receiving high-quality genome sequencing, accurate domain modeling that is representative of species diversity is crucial for understanding protein family sequence evolution and their inferred function(s). Here, we describe a bioinformatic tool called Taxon-Informed Adjustment of Markov Model Attributes (TIAMMAt) which revises domain profile hidden Markov models (HMMs) by incorporating homologous domain sequences from underrepresented and nonmodel species. Using innate immunity pathways as a case study, we show that revising profile HMM parameters to directly account for variation in homologs among underrepresented species provides valuable insight into the evolution of protein families. Following adjustment by TIAMMAt, domain profile HMMs exhibit changes in their per-site amino acid state emission probabilities and insertion/deletion probabilities while maintaining the overall structure of the consensus sequence. Our results show that domain revision can heavily impact evolutionary interpretations for some families (i.e., NLR’s NACHT domain), whereas impact on other domains (e.g., rel homology domain and interferon regulatory factor domains) is minimal due to high levels of sequence conservation across the sampled phylogenetic depth (i.e., Metazoa). Importantly, TIAMMAt revises target domain models to reflect homologous sequence variation using the taxonomic distribution under consideration by the user. TIAMMAt’s flexibility to revise any subset of the Pfam database using a user-defined taxonomic pool will make it a valuable tool for future protein evolution studies, particularly when incorporating (or focusing) on nonmodel species.

Beschreibung: This work was supported by The National Science Foundation (Grant No. IOS—1755377 to K.M.H., Rita Graze, and Elizabeth Hiltbold Schwartz), and K.T.D. was supported by The National Science Foundation’s Graduate Research Fellowship Program.

Beschreibung: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Ruff, S. E. Editorial: microbial communities and metabolisms involved in the degradation of cellular and extracellular organic biopolymers. Frontiers in Microbiology, 12, (2022): 802619, https://doi.org/10.3389/fmicb.2021.802619.

Beschreibung: Most organic matter on Earth occurs in the form of macromolecules and complex biopolymers, which include the building blocks of every organism. Plant, animal, fungal, and microbial cells largely consist of macromolecules belonging to four compound classes: proteins, polysaccharides, nucleic acids, and lipids (Figure 1). The percentage of these compounds per dry weight can vary greatly between lineages, but also between individuals of the same species or developmental stages of the same organism. Living and lysing cells release a substantial quantity and variety of macromolecules to the environment. These compounds often contain nitrogen, phosphorus, and sulfur, in addition to carbon, and are thus ideal food sources for heterotrophic organisms. Although the degradation of biopolymers and macromolecules has received considerable attention, many knowledge gaps remain, particularly in very complex ecosystems such as soils and sediments.

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Klein, S., Frazier, V., Readdean, T., Lucas, E., Diaz-Jimenez, E. P., Sogin, M., Ruff, E. S., & Echeverri, K. Common environmental pollutants negatively affect development and regeneration in the sea anemone Nematostella vectensis holobiont. Frontiers in Ecology and Evolution, 9, (2021): 786037, https://doi.org/10.3389/fevo.2021.786037.

Beschreibung: The anthozoan sea anemone Nematostella vectensis belongs to the phylum of cnidarians which also includes jellyfish and corals. Nematostella are native to United States East Coast marsh lands, where they constantly adapt to changes in salinity, temperature, oxygen concentration and pH. Its natural ability to continually acclimate to changing environments coupled with its genetic tractability render Nematostella a powerful model organism in which to study the effects of common pollutants on the natural development of these animals. Potassium nitrate, commonly used in fertilizers, and Phthalates, a component of plastics are frequent environmental stressors found in coastal and marsh waters. Here we present data showing how early exposure to these pollutants lead to dramatic defects in development of the embryos and eventual mortality possibly due to defects in feeding ability. Additionally, we examined the microbiome of the animals and identified shifts in the microbial community that correlated with the type of water that was used to grow the animals, and with their exposure to pollutants.

Beschreibung: This work was funded by a Pilot Program award to ER and KE from the Microbiome Center at the University of Chicago. The microbiome sequencing was funded by a grant from the McDonnell Initiative to ER. KE was supported by a grant from NICHD R01 HD092451, start-up funds from the MBL and funding from the Owens Family Foundation. ER was supported by start-up funds from the MBL and MLS receives support from the Unger G. Vetlesen Foundation.

Beschreibung: © The Author(s), 2022. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Katz, H. R., Arcese, A. A., Bloom, O., & Morgan, J. R. Activating transcription factor 3 (ATF3) is a highly conserved pro-regenerative transcription factor in the vertebrate nervous system. Frontiers in Cell and Developmental Biology, 10, (2022): 824036, https://doi.org/10.3389/fcell.2022.824036.

Beschreibung: The vertebrate nervous system exhibits dramatic variability in regenerative capacity across species and neuronal populations. For example, while the mammalian central nervous system (CNS) is limited in its regenerative capacity, the CNS of many other vertebrates readily regenerates after injury, as does the peripheral nervous system (PNS) of mammals. Comparing molecular responses across species and tissues can therefore provide valuable insights into both conserved and distinct mechanisms of successful regeneration. One gene that is emerging as a conserved pro-regenerative factor across vertebrates is activating transcription factor 3 (ATF3), which has long been associated with tissue trauma. A growing number of studies indicate that ATF3 may actively promote neuronal axon regrowth and regeneration in species ranging from lampreys to mammals. Here, we review data on the structural and functional conservation of ATF3 protein across species. Comparing RNA expression data across species that exhibit different abilities to regenerate their nervous system following traumatic nerve injury reveals that ATF3 is consistently induced in neurons within the first few days after injury. Genetic deletion or knockdown of ATF3 expression has been shown in mouse and zebrafish, respectively, to reduce axon regeneration, while inducing ATF3 promotes axon sprouting, regrowth, or regeneration. Thus, we propose that ATF3 may be an evolutionarily conserved regulator of neuronal regeneration. Identifying downstream effectors of ATF3 will be a critical next step in understanding the molecular basis of vertebrate CNS regeneration.

Beschreibung: This work was supported by: a Morton Cure Paralysis Fund Research Grant (to HK); a NIH/NINDS R03 Research Grant (No. 1R03NS078519) and the New York State Spinal Cord Injury Research Board (to OB); and the Marine Biological Laboratory Eugene Bell Center Endowment, Rowe Endowment for Regenerative Biology, and Charles Evans Research Development award (to JM).

Beschreibung: © The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Richter-Heitmann, T., Hofner, B., Krah, F., Sikorski, J., Wuest, P. K., Bunk, B., Huang, S., Regan, K. M., Berner, D., Boeddinghaus, R. S., Marhan, S., Prati, D., Kandeler, E., Overmann, J., & Friedrich, M. W. Stochastic dispersal rather than deterministic selection explains the spatio-temporal distribution of soil bacteria in a temperate grassland. Frontiers in Microbiology, 11, (2020): 1391, https://doi.org/10.3389/fmicb.2020.01391.

Beschreibung: Spatial and temporal processes shaping microbial communities are inseparably linked but rarely studied together. By Illumina 16S rRNA sequencing, we monitored soil bacteria in 360 stations on a 100 square meter plot distributed across six intra-annual samplings in a rarely managed, temperate grassland. Using a multi-tiered approach, we tested the extent to which stochastic or deterministic processes influenced the composition of local communities. A combination of phylogenetic turnover analysis and null modeling demonstrated that either homogenization by unlimited stochastic dispersal or scenarios, in which neither stochastic processes nor deterministic forces dominated, explained local assembly processes. Thus, the majority of all sampled communities (82%) was rather homogeneous with no significant changes in abundance-weighted composition. However, we detected strong and uniform taxonomic shifts within just nine samples in early summer. Thus, community snapshots sampled from single points in time or space do not necessarily reflect a representative community state. The potential for change despite the overall homogeneity was further demonstrated when the focus shifted to the rare biosphere. Rare OTU turnover, rather than nestedness, characterized abundance-independent β-diversity. Accordingly, boosted generalized additive models encompassing spatial, temporal and environmental variables revealed strong and highly diverse effects of space on OTU abundance, even within the same genus. This pure spatial effect increased with decreasing OTU abundance and frequency, whereas soil moisture – the most important environmental variable – had an opposite effect by impacting abundant OTUs more than the rare ones. These results indicate that – despite considerable oscillation in space and time – the abundant and resident OTUs provide a community backbone that supports much higher β-diversity of a dynamic rare biosphere. Our findings reveal complex interactions among space, time, and environmental filters within bacterial communities in a long-established temperate grassland.

Beschreibung: The work has been funded by the DFG Priority Program 1374 “Infrastructure-Biodiversity-Exploratories” (Grants KA 1590/8-2, FR 1151/5-2, and OV 20/21-1). Field work permits were issued by the responsible state environmental office of Baden-Württemberg (according to § 72 BbgNatSchG).

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Mueller, R. L., Combs, B., Alhadidy, M. M., Brady, S. T., Morfini, G. A., & Kanaan, N. M. Tau: a signaling hub protein. Frontiers in Molecular Neuroscience, 14, (2021): 647054, https://doi.org/10.3389/fnmol.2021.647054.

Beschreibung: Over four decades ago, in vitro experiments showed that tau protein interacts with and stabilizes microtubules in a phosphorylation-dependent manner. This observation fueled the widespread hypotheses that these properties extend to living neurons and that reduced stability of microtubules represents a major disease-driving event induced by pathological forms of tau in Alzheimer’s disease and other tauopathies. Accordingly, most research efforts to date have addressed this protein as a substrate, focusing on evaluating how specific mutations, phosphorylation, and other post-translational modifications impact its microtubule-binding and stabilizing properties. In contrast, fewer efforts were made to illuminate potential mechanisms linking physiological and disease-related forms of tau to the normal and pathological regulation of kinases and phosphatases. Here, we discuss published work indicating that, through interactions with various kinases and phosphatases, tau may normally act as a scaffolding protein to regulate phosphorylation-based signaling pathways. Expanding on this concept, we also review experimental evidence linking disease-related tau species to the misregulation of these pathways. Collectively, the available evidence supports the participation of tau in multiple cellular processes sustaining neuronal and glial function through various mechanisms involving the scaffolding and regulation of selected kinases and phosphatases at discrete subcellular compartments. The notion that the repertoire of tau functions includes a role as a signaling hub should widen our interpretation of experimental results and increase our understanding of tau biology in normal and disease conditions.

Beschreibung: This work was supported by NIH grants (R01AG067762 and R01AG044372 to NK, R01NS082730 to NK and SB, R01NS118177 and R21NS120126 to GM, R01NS023868 and R01NS041170 to SB), a gift from Neurodegenerative Research Inc. (GM), a Zenith Award from the Alzheimer’s Association (SB), a grant from the Secchia Family Foundation (NK), NIH/National Institute on Aging (NIA) funded Michigan Alzheimer’s Disease Research Center 5P30AG053760 (BC), the Office of the Assistant Secretary of Defense for Health Affairs through the Peer-Reviewed Alzheimer’s Research Program (Award No. W81XWH-20-1-0174 to BC), and an Alzheimer’s Association Research Grant 20-682085 (BC).

Beschreibung: © The Author(s), 2021. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Chowdhury, P. R., Golas, S. M., Alteio, L., Stevens, J. T. E., Billings, A. F., Blanchard, J. L., Melillo, J. M., & DeAngelis, K. M. The transcriptional response of soil bacteria to long-term warming and short-term seasonal fluctuations in a terrestrial forest. Frontiers in Microbiology, 12, (2021): 666558, https://doi.org/10.3389/fmicb.2021.666558.

Beschreibung: Terrestrial ecosystems are an important carbon store, and this carbon is vulnerable to microbial degradation with climate warming. After 30 years of experimental warming, carbon stocks in a temperate mixed deciduous forest were observed to be reduced by 30% in the heated plots relative to the controls. In addition, soil respiration was seasonal, as was the warming treatment effect. We therefore hypothesized that long-term warming will have higher expressions of genes related to carbohydrate and lipid metabolism due to increased utilization of recalcitrant carbon pools compared to controls. Because of the seasonal effect of soil respiration and the warming treatment, we further hypothesized that these patterns will be seasonal. We used RNA sequencing to show how the microbial community responds to long-term warming (~30 years) in Harvard Forest, MA. Total RNA was extracted from mineral and organic soil types from two treatment plots (+5°C heated and ambient control), at two time points (June and October) and sequenced using Illumina NextSeq technology. Treatment had a larger effect size on KEGG annotated transcripts than on CAZymes, while soil types more strongly affected CAZymes than KEGG annotated transcripts, though effect sizes overall were small. Although, warming showed a small effect on overall CAZymes expression, several carbohydrate-associated enzymes showed increased expression in heated soils (~68% of all differentially expressed transcripts). Further, exploratory analysis using an unconstrained method showed increased abundances of enzymes related to polysaccharide and lipid metabolism and decomposition in heated soils. Compared to long-term warming, we detected a relatively small effect of seasonal variation on community gene expression. Together, these results indicate that the higher carbohydrate degrading potential of bacteria in heated plots can possibly accelerate a self-reinforcing carbon cycle-temperature feedback in a warming climate.

Beschreibung: Funding for this study was provided by the Department of Energy Terrestrial Ecosystem Sciences program under contract number DE-SC0010740. Sites for sample collection were maintained with funding in part from the National Science Foundation (NSF) Long-Term Ecological Research (DEB 1237491) and the NSF Long-Term Research in Environmental Biology (DEB 1456528) programs.